The brokering approach can be successfully used to overcome the crucial question of searching among enormous amount of data （raw and/or processed） produced and stored in different information systems. In this paper,...The brokering approach can be successfully used to overcome the crucial question of searching among enormous amount of data （raw and/or processed） produced and stored in different information systems. In this paper, authors describe the Data Management System the DMS （Data Management System） developed by INGV （Istituto Nazionale di Geofisica e Vulcanologia） to support the brokering system GEOSS （Global Earth Observation System of Systems） adopted for the ARCA （Arctic Present Climate Change and Past Extreme Events） project. This DMS includes heterogeneous data that contributes to the ARCA objective （www.arcaproject.it） focusing on multi-parametric and multi-disciplinary studies on the mechanism （s） behind the release of large volumes of cold and fresh water from melting of ice caps. The DMS is accessible directly at the www.arca.rm.ingv.it, or through the IADC （Italian Arctic Data Center） at http：//arcticnode.dta.cnr.it/iadc/gi-portal/index.jsp that interoperates with the GEOSS brokering system （http：//www.geoportal.org0 making easy and fast the search of specific data set and its URL.展开更多
Alzheimer’s disease(AD)and other tauopathies are characterized by the accumulation of misfolded tau protein,which forms toxic oligomers that contribute to synaptic dysfunction and neuronal loss.Here,we briefly discus...Alzheimer’s disease(AD)and other tauopathies are characterized by the accumulation of misfolded tau protein,which forms toxic oligomers that contribute to synaptic dysfunction and neuronal loss.Here,we briefly discuss recent findings indicating that the cellular prion protein(PrPC)plays a critical role in mediating the synaptotoxic effects of tau oligomers(TauOs),offering new insights into disease pathogenesis and potential therapeutic strategies.展开更多
Introduction:One of the main events that regulate a cell’s well-being is cell-to-cell communication.This intercellular mechanism of information transfer is often mediated by vesicular trafficking.Mitochondrial-derive...Introduction:One of the main events that regulate a cell’s well-being is cell-to-cell communication.This intercellular mechanism of information transfer is often mediated by vesicular trafficking.Mitochondrial-derived vesicles(MDVs)are an emerging subpopulation of extracellular vesicle(EV)first discovered in 2008 that allow mitochondria to communicate with their surroundings.展开更多
Background:The Da Vinci Single-Port Robotic System(Da Vinci-SP),introduced by Intuitive(CA,USA)in 2018 in the USA and in 2024 in Europe,integrates advanced features like a flexible camera and articulating instruments....Background:The Da Vinci Single-Port Robotic System(Da Vinci-SP),introduced by Intuitive(CA,USA)in 2018 in the USA and in 2024 in Europe,integrates advanced features like a flexible camera and articulating instruments.It has garnered significant interest in urology.Our report presents the first described European series of Radical Prostatectomies using the Da Vinci SP at the leading Italian center,Istituto Nazionale Tumori di Napoli,IRCCS“G.Pascale”Foundation,detailing the technical differences and challenges faced by experienced multiport robotic surgeons.Methods:Sixteen patients have been enrolled and underwent Single-Port(SP)Robot-Assisted Radical Prostatectomy(SP-RARP).Baseline characteristics of the patients were collected.We provided a step-by-step description of the surgical technique.Oncological outcomes have been evaluated and compared with magnetic resonance imaging(MRI)and biopsy results.Intraoperative,perioperative,and postoperative complications,surgical outcomes,functional outcomes,and technical issues of the new system were also documented.Results:All surgeries were successfully performed without the need for conversion.An extraperitoneal approach was used for all patients.Median Console time was 110 min.No complications were reported.The estimated median blood loss was 175 cc.Discharge from the hospital was on the first post-operative day for all patients.Bladder catheter removal was on day 7 without the need for cystography.Conclusions:We presented the first European case series of SP-RARP,reporting our experience and confirming the procedure’s feasibility for a highly experienced robotic surgeon.Experience with an extraperitoneal approach using the multiport(MP)platform,combined with well-conducted training for the SP system,may facilitate the transition to SP surgery.Further procedures and studies are needed to evaluate the oncological and functional outcomes.展开更多
The Arno River Basin(Central Italy)is affected by a considerable anthropogenic pressure due to the presence of large cities and widespread industrial and agricultural practices.In this work,26 water samples from the A...The Arno River Basin(Central Italy)is affected by a considerable anthropogenic pressure due to the presence of large cities and widespread industrial and agricultural practices.In this work,26 water samples from the Arno River and its main tributaries were analyzed to assess the water pollution status.The geochemical composition of the Arno River changes from the source(dominated by a Ca-HCO_(3) facies)to the mouth(where a Na-Cl(SO4)chemistry prevails)with an increasing quality deterioration,as suggested by the Chemical Water Quality Index,due to anthropogenic contributions and seawater intrusion before flowing into the Ligurian Sea.The Ombrone and Usciana tributaries introduce anthropogenic pollutants into the Arno River,whilst Elsa tributary supplies significant contents of geogenic sulfate.The concentrations of dissolved nitrate and nitrite(up to 63 and 9 mg/L,respectively)and the respective isotopic values of𝛿15N and𝛿18O were also determined to understand origin and fate of the N-species in the Arno River Basin surface waters.The combined application of𝛿15N-NO_(3) and𝛿18O-NO_(3) and N-source apportionment modelling allowed the identification of soil organic nitrogen and sewage and domestic wastes as primary sources for dissolved NO_(3)-.The𝛿15N-NO_(2) and𝛿18O-NO_(2) values suggest that the nitrification process affects the ARB waters,thus controlling the abundances and proportion of the N-species.Our work indicates that additional efforts are needed to improve management strategies to reduce the release of nitrogenated species to the surface waters of the Arno River Basin,since little progress has been made from the early 2000s.展开更多
Copper is a transition metal and an essential element for the organism,as alterations in its homeostasis leading to metal accumulation or deficiency have pathological effects in several organs,including the central ne...Copper is a transition metal and an essential element for the organism,as alterations in its homeostasis leading to metal accumulation or deficiency have pathological effects in several organs,including the central nervous system.Central copper dysregulations have been evidenced in two genetic disorders characterized by mutations in the copper-ATPases ATP7A and ATP7B,Menkes disease and Wilson’s disease,respectively,and also in multifactorial neurological disorders such as Alzheimer’s disease,Parkinson’s disease,amyotrophic lateral sclerosis,and multiple sclerosis.This review summarizes current knowledge about the role of copper in central nervous system physiology and pathology,reports about unbalances in copper levels and/or distribution under disease,describes relevant animal models for human disorders where copper metabolism genes are dysregulated,and discusses relevant therapeutic approaches modulating copper availability.Overall,alterations in copper metabolism may contribute to the etiology of central nervous system disorders and represent relevant therapeutic targets to restore tissue homeostasis.展开更多
The brain,with its trillions of neural connections,different cellular types,and molecular complexities,presents a formidable challenge for researchers aiming to comprehend the multifaceted nature of neural health.As t...The brain,with its trillions of neural connections,different cellular types,and molecular complexities,presents a formidable challenge for researchers aiming to comprehend the multifaceted nature of neural health.As traditional methods have provided valuable insights,emerging technologies offer unprecedented opportunities to delve deeper into the underpinnings of brain function.In the everevolving landscape of neuroscience,the quest to unravel the mysteries of the human brain is bound to take a leap forward thanks to new technological improvements and bold interpretative frameworks.展开更多
BACKGROUND The CAR-OLT score predicts major adverse cardiovascular events 1 year after liver transplant(LT).AIM To test the hypothesis that the CAR-OLT score may help avoid cardiac stress tests in LT candidates.METHOD...BACKGROUND The CAR-OLT score predicts major adverse cardiovascular events 1 year after liver transplant(LT).AIM To test the hypothesis that the CAR-OLT score may help avoid cardiac stress tests in LT candidates.METHODS This retrospective single-center cohort study included all adult patients undergoing elective evaluation for first cadaveric donor orthotopic LT for liver cirrhosis with or without hepatocellular carcinoma at Fondazione Policlinico Universitario Agostino Gemelli Istituto di Ricerca e Cura a Carattere Scientifico in Rome,Italy.Cardiac contraindications for LT listing were defined after a center-specific cardiac workup,which included cardiac stress tests for most patients.The diagnostic accuracy of the CAR-OLT score was evaluated using the area under the receiver operating characteristic(AUROC)method.RESULTS A total of 342 LT candidates were evaluated between 2015 and 2019,with a moderate cardiovascular risk profile(37%diabetes,34%hypertension,22%obesity).Of these,80(23%)candidates underwent coronary angiography.Twenty-one(6%)candidates were given cardiac contraindications to LT listing,48%of which were due to coronary artery disease.The CAR-OLT score predicted cardiac contraindications to LT listing with an AUROC of 0.81.The optimal cut-off for sensitivity was a CAR-OLT score≤23,which showed a 99%negative predictive value for cardiac contraindications to LT listing.A total of 84(25%)LT candidates with a CAR-OLT score≤23 underwent 87 non-invasive cardiac tests and 13 coronary angiographies pre-listing,with estimated costs of approximately 48000€.The estimated savings per patient was€574.70 for the Italian National Health System.CONCLUSION A CAR-OLT score≤23 can identify LT candidates who can be safely listed without the need for cardiac stress tests,providing time and cost savings.These findings require external validation.展开更多
Astrocytes,the main population of glial cells in the central nervous system(CNS),exert essential tasks for the control of brain tissue homeostasis,supporting neuron and other glial cell activity from the developmental...Astrocytes,the main population of glial cells in the central nervous system(CNS),exert essential tasks for the control of brain tissue homeostasis,supporting neuron and other glial cell activity from the developmental stage to adult life.To maintain the optimal functionality of the brain,astroglial cells are particularly committed to reacting to every change in tissue homeostatic conditions,from mild modifications of the physiological environment,a process called astrocyte activation,to the more severe alterations occurring in pathological situations causing astrocyte reactivity or reactive astrogliosis(Escartin et al.,2021).During these reactive states,astrocytes mount an active,progressive response encompassing morphological,molecular,and interactional remodeling,leading to the acquisition of new functions and the loss of others,whose intensity,duration,and reversibility are dependent on the nature of the stimulus and regulated in a context-specific manner.展开更多
The 7 ka old Qixiangzhan lava flow(QXZ,Tianchi volcano)represents the last eruptive event before the 946 CE,caldera-forming‘Millennium’eruption(ME).Petrographic,whole rock,mineral composition,Sr-Nd isotopic data on ...The 7 ka old Qixiangzhan lava flow(QXZ,Tianchi volcano)represents the last eruptive event before the 946 CE,caldera-forming‘Millennium’eruption(ME).Petrographic,whole rock,mineral composition,Sr-Nd isotopic data on QXZ show that:(a)the lava consists of two components,constituted by comenditic obsidian fragments immersed in a continuous,aphanitic component;(b)both components have the same geochemical and isotopic variations of the ME magma.The QXZ and ME comendites result from fractional crystallization and crustal assimilation processes.The temperature of the QXZ magma was about 790℃ and the depth of the magma reservoir around 7 km,the same values as estimated for ME.QXZ had a viscosity of 10^(5.5)-10^(9) Pa s and a velocity of 3-10 km/yr.The emplacement time was 0.5-1.6yr and the flow rate 0.48-1.50 m^(3)/s.These values lie within the range estimated for other rhyolitic flows worldwide.The QXZ lava originated through a mixed explosive-effusive activity with the obsidian resulting from the ascent of undercooling,degassing and the fragmentation of magma along the conduit walls,whereas the aphanitic component testifies to the less undercooled and segregated flow at the center of the conduit.The QXZ lava demonstrates the extensive history of the ME magma chamber.展开更多
PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other mammals.PrPSccan adopt different patho...PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other mammals.PrPSccan adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective therapies.Since PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion diseases.In this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of action.We argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.展开更多
BACKGROUND Enhanced recovery after surgery(ERAS)programs provide recommendations for an optimized management of patients undergoing surgery.An ERAS program tailored on surgery for retroperitoneal sarcomas(RPS)may impr...BACKGROUND Enhanced recovery after surgery(ERAS)programs provide recommendations for an optimized management of patients undergoing surgery.An ERAS program tailored on surgery for retroperitoneal sarcomas(RPS)may improve patient outcomes and it has still not been established.AIM To determine how an ERAS program tailored to RPS surgery can be agreed upon,structured,and implemented.METHODS Twenty-five candidate items from existing ERAS programs,potentially relevant for RPS surgery,were identified via literature review and expert input.These were included in a questionnaire refined through cognitive interviews and pilot testing.Expert sarcoma surgeons rated each item’s relevance and feasibility on a 6-point scale.The survey was recirculated after one year.Intra-observer reproducibility,inter-observer concordance,and agreement with the modal value of the most experienced participants were analyzed.RESULTS Thirteen sarcoma surgeons from 6 centers participated in the survey.Although surgeons agreed on several items,their overall concordance was low.After recirculating the survey,the intraobserver reproducibility was low.Interestingly,the median concordance with the reference increased for relevance and decreased for feasibility.CONCLUSION Despite interest in ERAS for RPS,surgeon concordance on item relevance and feasibility remains low,underscoring the need for collaborative efforts toward a standardized,consensus-based protocol.展开更多
All-inorganic perovskites based on cesium-lead-bromine(Cs-Pb-Br)have been a prominent research focus in optoelectronics in recent years.The optimisation and tunability of their macroscopic properties exploit the confo...All-inorganic perovskites based on cesium-lead-bromine(Cs-Pb-Br)have been a prominent research focus in optoelectronics in recent years.The optimisation and tunability of their macroscopic properties exploit the conformational flexibility,resulting in various crystal structures.Varying synthesis parameters can yield distinct crystal structures from Cs,Pb,and Br precursors,and manually exploring the relationship between these synthesis parameters and the resulting crystal structure is both labour-intensive and time-consuming.Machine learning(ML)can rapidly uncover insights and drive discoveries in chemical synthesis with the support of data,significantly reducing both the cost and development cycle of materials.Here,we gathered synthesis parameters from published literature(220 synthesis runs)and implemented eight distinct ML models,including eXtreme Gradient Boosting(XGB),Decision Tree(DT),Support Vector Machine(SVM),Random Forest(RF),Naïve Bayes(NB),Logistic Regression(LR),Gradient Boosting(GB),and K-Nearest(KN)to classify and predict Cs-Pb-Br crystal structures from given synthesis parameters.Validation accuracy,precision,F1 score,recall,and average area under the curve(AUC)are employed to evaluate these ML models.The XGB model exhibited the best performance,achieving a validation accuracy of 0.841.The trained XGB model was subsequently utilised to predict the structure from 10 experimental runs using a randomised set of parameters,achieving a testing accuracy of 0.8.The results indicate that the Cs/Pb molar ratio,reaction time,and the concentration of organic compounds(ligands)play crucial roles in synthesising various crystal structures of Cs-Pb-Br.This study demonstrates a significant decrease in effort required for experimental procedures and builds a foundational basis for predicting crystal structures from synthesis parameters.展开更多
Cenozoic trachytes are characteristic of some active volcanic fields in China.In particular,the origin and mechanisms of the evolution of trachytes from the Tianchi(Changbaishan)volcano(TV,China/North Korea)are poorly...Cenozoic trachytes are characteristic of some active volcanic fields in China.In particular,the origin and mechanisms of the evolution of trachytes from the Tianchi(Changbaishan)volcano(TV,China/North Korea)are poorly known.Here,we present new geochronological,geochemical and isotopic data on two trachytic suites outcropping on the northern and southern upper slopes of TV.Detailed zircon laser ablation-multicollector-inductively coupled plasma-mass spectrometry(LA-MC-ICP-MS)U-Pb dating,Rb-Sr isochron dating of plagioclase and hornblende,^(40)Ar/^(39)Ar chronology with mineral chemistry,whole-rock element and Sr-Nd-Pb isotope data are used to explore their origins and evolutionary mechanisms during the late Middle Pleistocene.Our data indicate that the trachytes mainly consist of sanidine,orthoclase and plagioclase,with minor albite,quartz and hornblende.They formed at 0.353-0.346 Ma(lava flow from the northern slope)and 0.383-0.311 Ma(lava flow from the southern slope),respectively.The TV trachytes are characterized by high K_(2)O/Na_(2)O and AR values,with low A/CNK and Mg~#values.They are enriched in rare earth elements(REEs;except Eu),depleted in Sr and Ba,crystallizing at 742-858℃.The TV trachytes have high(^(87)Sr/^(86)Sr)_i values(0.70776-0.71195),positiveε_(Nd)(t)values(0.61-2.93)and radiogenic^(206)Pb/^(204)Pb values(17.515-17.806).These values are similar to those of the Pleistocene and Holocene TV trachytes.Geochemical data indicate that they were formed by fractional crystallization from a basaltic melt and assimilated upper crust material.The trace element pattern of the studied trachytes is consistent with an evolution from basaltic melts representative of an enriched mantle source.The vent from which the trachytic magma was erupted probably collapsed inside the caldera during the TV'Millennium'eruption(ME)in 946 AD.The contemporaneous emission of basaltic and trachytic magma during the Middle-Late Pleistocene suggests the coexistence of fissural basaltic volcanism and central-type trachytic volcanism,the latter of which was associated with a magma chamber in the upper crust during the TV cone-construction stage.展开更多
BACKGROUND Median sternotomy has been considered the gold standard approach for anterior mediastinal tumor resection.However,recent advances in video-assisted thoracoscopic surgery and robotic-assisted thoracoscopic s...BACKGROUND Median sternotomy has been considered the gold standard approach for anterior mediastinal tumor resection.However,recent advances in video-assisted thoracoscopic surgery and robotic-assisted thoracoscopic surgery with carbon dioxide insufflation have allowed minimally invasive approaches even for large and locally invasive tumors of the upper-anterior mediastinum.The subxiphoid robotic optical approach is a recently developed technique for accessing the mediastinum.The trans-subxiphoid technique offers excellent exposure of the surgical field,reduces postoperative pain,facilitates specimen retrieval even for large tumors,and potentially improves early surgical outcomes.AIM To evaluate the safety,feasibility,and outcomes of a robotic subxiphoid approach for the resecting of large/invasive mediastinal tumors.METHODS Between July 2024 and September 2025,12 patients underwent subxiphoid robotic mediastinal resection.The diameter of the operated lesions ranged from 30 mm to 70 mm.A 3 cm subxiphoid incision was made at the subxiphoid level for GelPort placement,allowing for optical port access.Two operating ports were placed at the sixth intercostal space bilaterally.Carbon dioxide insufflations(8-10 mmHg)enlarged the surgical field,improving visualization of critical anatomical landmarks,such as the internal mammary arteries and phrenic nerves.This approach allowed complete resection of large or invasive tumors,preserving thoracic stability and reducing the risk of postoperative myasthenic crisis.RESULTS The mean operating time was 170.2 minutes,and the median hospital stay was 3.5 days.No major postoperative complications occurred.Two conversions were necessary:One with a lateral robotic approach due to previous abdominal surgery,and one with a sternotomy for tumor invasion of the aortic arch.Histopathological analysis identified nine thymomas and one solitary fibrous tumor.CONCLUSION Subxiphoid robotic approach is a safe,effective technique for extended thymectomy,fulfilling both oncological and myasthenia gravis surgical objectives.展开更多
文摘The brokering approach can be successfully used to overcome the crucial question of searching among enormous amount of data （raw and/or processed） produced and stored in different information systems. In this paper, authors describe the Data Management System the DMS （Data Management System） developed by INGV （Istituto Nazionale di Geofisica e Vulcanologia） to support the brokering system GEOSS （Global Earth Observation System of Systems） adopted for the ARCA （Arctic Present Climate Change and Past Extreme Events） project. This DMS includes heterogeneous data that contributes to the ARCA objective （www.arcaproject.it） focusing on multi-parametric and multi-disciplinary studies on the mechanism （s） behind the release of large volumes of cold and fresh water from melting of ice caps. The DMS is accessible directly at the www.arca.rm.ingv.it, or through the IADC （Italian Arctic Data Center） at http：//arcticnode.dta.cnr.it/iadc/gi-portal/index.jsp that interoperates with the GEOSS brokering system （http：//www.geoportal.org0 making easy and fast the search of specific data set and its URL.
基金supported by the Italian Ministry of Health grant RF-2021-12372337(to GF and CB).
文摘Alzheimer’s disease(AD)and other tauopathies are characterized by the accumulation of misfolded tau protein,which forms toxic oligomers that contribute to synaptic dysfunction and neuronal loss.Here,we briefly discuss recent findings indicating that the cellular prion protein(PrPC)plays a critical role in mediating the synaptotoxic effects of tau oligomers(TauOs),offering new insights into disease pathogenesis and potential therapeutic strategies.
基金supported by project Emerging Infectious Diseases One Health Basic and Translational Research Actions addressing Unmet Needs on Emerging Infectious Diseases,INF-ACT,Spoke 1 and Spoke 5,Project number PE00000007,CUP B53C20040570005(to PP and DN).
文摘Introduction:One of the main events that regulate a cell’s well-being is cell-to-cell communication.This intercellular mechanism of information transfer is often mediated by vesicular trafficking.Mitochondrial-derived vesicles(MDVs)are an emerging subpopulation of extracellular vesicle(EV)first discovered in 2008 that allow mitochondria to communicate with their surroundings.
文摘Background:The Da Vinci Single-Port Robotic System(Da Vinci-SP),introduced by Intuitive(CA,USA)in 2018 in the USA and in 2024 in Europe,integrates advanced features like a flexible camera and articulating instruments.It has garnered significant interest in urology.Our report presents the first described European series of Radical Prostatectomies using the Da Vinci SP at the leading Italian center,Istituto Nazionale Tumori di Napoli,IRCCS“G.Pascale”Foundation,detailing the technical differences and challenges faced by experienced multiport robotic surgeons.Methods:Sixteen patients have been enrolled and underwent Single-Port(SP)Robot-Assisted Radical Prostatectomy(SP-RARP).Baseline characteristics of the patients were collected.We provided a step-by-step description of the surgical technique.Oncological outcomes have been evaluated and compared with magnetic resonance imaging(MRI)and biopsy results.Intraoperative,perioperative,and postoperative complications,surgical outcomes,functional outcomes,and technical issues of the new system were also documented.Results:All surgeries were successfully performed without the need for conversion.An extraperitoneal approach was used for all patients.Median Console time was 110 min.No complications were reported.The estimated median blood loss was 175 cc.Discharge from the hospital was on the first post-operative day for all patients.Bladder catheter removal was on day 7 without the need for cystography.Conclusions:We presented the first European case series of SP-RARP,reporting our experience and confirming the procedure’s feasibility for a highly experienced robotic surgeon.Experience with an extraperitoneal approach using the multiport(MP)platform,combined with well-conducted training for the SP system,may facilitate the transition to SP surgery.Further procedures and studies are needed to evaluate the oncological and functional outcomes.
文摘The Arno River Basin(Central Italy)is affected by a considerable anthropogenic pressure due to the presence of large cities and widespread industrial and agricultural practices.In this work,26 water samples from the Arno River and its main tributaries were analyzed to assess the water pollution status.The geochemical composition of the Arno River changes from the source(dominated by a Ca-HCO_(3) facies)to the mouth(where a Na-Cl(SO4)chemistry prevails)with an increasing quality deterioration,as suggested by the Chemical Water Quality Index,due to anthropogenic contributions and seawater intrusion before flowing into the Ligurian Sea.The Ombrone and Usciana tributaries introduce anthropogenic pollutants into the Arno River,whilst Elsa tributary supplies significant contents of geogenic sulfate.The concentrations of dissolved nitrate and nitrite(up to 63 and 9 mg/L,respectively)and the respective isotopic values of𝛿15N and𝛿18O were also determined to understand origin and fate of the N-species in the Arno River Basin surface waters.The combined application of𝛿15N-NO_(3) and𝛿18O-NO_(3) and N-source apportionment modelling allowed the identification of soil organic nitrogen and sewage and domestic wastes as primary sources for dissolved NO_(3)-.The𝛿15N-NO_(2) and𝛿18O-NO_(2) values suggest that the nitrification process affects the ARB waters,thus controlling the abundances and proportion of the N-species.Our work indicates that additional efforts are needed to improve management strategies to reduce the release of nitrogenated species to the surface waters of the Arno River Basin,since little progress has been made from the early 2000s.
文摘Copper is a transition metal and an essential element for the organism,as alterations in its homeostasis leading to metal accumulation or deficiency have pathological effects in several organs,including the central nervous system.Central copper dysregulations have been evidenced in two genetic disorders characterized by mutations in the copper-ATPases ATP7A and ATP7B,Menkes disease and Wilson’s disease,respectively,and also in multifactorial neurological disorders such as Alzheimer’s disease,Parkinson’s disease,amyotrophic lateral sclerosis,and multiple sclerosis.This review summarizes current knowledge about the role of copper in central nervous system physiology and pathology,reports about unbalances in copper levels and/or distribution under disease,describes relevant animal models for human disorders where copper metabolism genes are dysregulated,and discusses relevant therapeutic approaches modulating copper availability.Overall,alterations in copper metabolism may contribute to the etiology of central nervous system disorders and represent relevant therapeutic targets to restore tissue homeostasis.
文摘The brain,with its trillions of neural connections,different cellular types,and molecular complexities,presents a formidable challenge for researchers aiming to comprehend the multifaceted nature of neural health.As traditional methods have provided valuable insights,emerging technologies offer unprecedented opportunities to delve deeper into the underpinnings of brain function.In the everevolving landscape of neuroscience,the quest to unravel the mysteries of the human brain is bound to take a leap forward thanks to new technological improvements and bold interpretative frameworks.
文摘BACKGROUND The CAR-OLT score predicts major adverse cardiovascular events 1 year after liver transplant(LT).AIM To test the hypothesis that the CAR-OLT score may help avoid cardiac stress tests in LT candidates.METHODS This retrospective single-center cohort study included all adult patients undergoing elective evaluation for first cadaveric donor orthotopic LT for liver cirrhosis with or without hepatocellular carcinoma at Fondazione Policlinico Universitario Agostino Gemelli Istituto di Ricerca e Cura a Carattere Scientifico in Rome,Italy.Cardiac contraindications for LT listing were defined after a center-specific cardiac workup,which included cardiac stress tests for most patients.The diagnostic accuracy of the CAR-OLT score was evaluated using the area under the receiver operating characteristic(AUROC)method.RESULTS A total of 342 LT candidates were evaluated between 2015 and 2019,with a moderate cardiovascular risk profile(37%diabetes,34%hypertension,22%obesity).Of these,80(23%)candidates underwent coronary angiography.Twenty-one(6%)candidates were given cardiac contraindications to LT listing,48%of which were due to coronary artery disease.The CAR-OLT score predicted cardiac contraindications to LT listing with an AUROC of 0.81.The optimal cut-off for sensitivity was a CAR-OLT score≤23,which showed a 99%negative predictive value for cardiac contraindications to LT listing.A total of 84(25%)LT candidates with a CAR-OLT score≤23 underwent 87 non-invasive cardiac tests and 13 coronary angiographies pre-listing,with estimated costs of approximately 48000€.The estimated savings per patient was€574.70 for the Italian National Health System.CONCLUSION A CAR-OLT score≤23 can identify LT candidates who can be safely listed without the need for cardiac stress tests,providing time and cost savings.These findings require external validation.
基金supported by funds from the Italian Ministry of Health,Ricerca Finalizzata,(Grant N.GR-2013-02355882 and GR-2021-12373946 to AL)5x1000 Project of the Istituto Superiore di Sanità(Project code:ISS5x1000_21-949432e8c9be to AL)the European Union–NextGeneration EU through the Italian Ministry of University and Research under PNRR-M4C2-I1.3 Project PE_00000019“HEAL ITALIA”to EA(CUP I83C22001830006)。
文摘Astrocytes,the main population of glial cells in the central nervous system(CNS),exert essential tasks for the control of brain tissue homeostasis,supporting neuron and other glial cell activity from the developmental stage to adult life.To maintain the optimal functionality of the brain,astroglial cells are particularly committed to reacting to every change in tissue homeostatic conditions,from mild modifications of the physiological environment,a process called astrocyte activation,to the more severe alterations occurring in pathological situations causing astrocyte reactivity or reactive astrogliosis(Escartin et al.,2021).During these reactive states,astrocytes mount an active,progressive response encompassing morphological,molecular,and interactional remodeling,leading to the acquisition of new functions and the loss of others,whose intensity,duration,and reversibility are dependent on the nature of the stimulus and regulated in a context-specific manner.
基金funded by the National Natural Science Foundation of China(Grant Nos.41972313 and 41790453)the Engineering Research Center of Geothermal Resources Development Technology and Equipment,Ministry of Education,Jilin University。
文摘The 7 ka old Qixiangzhan lava flow(QXZ,Tianchi volcano)represents the last eruptive event before the 946 CE,caldera-forming‘Millennium’eruption(ME).Petrographic,whole rock,mineral composition,Sr-Nd isotopic data on QXZ show that:(a)the lava consists of two components,constituted by comenditic obsidian fragments immersed in a continuous,aphanitic component;(b)both components have the same geochemical and isotopic variations of the ME magma.The QXZ and ME comendites result from fractional crystallization and crustal assimilation processes.The temperature of the QXZ magma was about 790℃ and the depth of the magma reservoir around 7 km,the same values as estimated for ME.QXZ had a viscosity of 10^(5.5)-10^(9) Pa s and a velocity of 3-10 km/yr.The emplacement time was 0.5-1.6yr and the flow rate 0.48-1.50 m^(3)/s.These values lie within the range estimated for other rhyolitic flows worldwide.The QXZ lava originated through a mixed explosive-effusive activity with the obsidian resulting from the ascent of undercooling,degassing and the fragmentation of magma along the conduit walls,whereas the aphanitic component testifies to the less undercooled and segregated flow at the center of the conduit.The QXZ lava demonstrates the extensive history of the ME magma chamber.
基金supported by Telethon Italy award GGP15225(to RC and GM)Italian Ministry of Health award RF-2016-02362950(to RC and CZ)+1 种基金the CJD Foundation USA(to RC)the Associazione Italiana Encefalopatie da Prioni(AIEnP)(to RC).
文摘PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other mammals.PrPSccan adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective therapies.Since PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion diseases.In this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of action.We argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.
文摘BACKGROUND Enhanced recovery after surgery(ERAS)programs provide recommendations for an optimized management of patients undergoing surgery.An ERAS program tailored on surgery for retroperitoneal sarcomas(RPS)may improve patient outcomes and it has still not been established.AIM To determine how an ERAS program tailored to RPS surgery can be agreed upon,structured,and implemented.METHODS Twenty-five candidate items from existing ERAS programs,potentially relevant for RPS surgery,were identified via literature review and expert input.These were included in a questionnaire refined through cognitive interviews and pilot testing.Expert sarcoma surgeons rated each item’s relevance and feasibility on a 6-point scale.The survey was recirculated after one year.Intra-observer reproducibility,inter-observer concordance,and agreement with the modal value of the most experienced participants were analyzed.RESULTS Thirteen sarcoma surgeons from 6 centers participated in the survey.Although surgeons agreed on several items,their overall concordance was low.After recirculating the survey,the intraobserver reproducibility was low.Interestingly,the median concordance with the reference increased for relevance and decreased for feasibility.CONCLUSION Despite interest in ERAS for RPS,surgeon concordance on item relevance and feasibility remains low,underscoring the need for collaborative efforts toward a standardized,consensus-based protocol.
基金the Italian Space Agency(Agenzia Spaziale Italiana,ASI)in the framework of the Research Day“Giornate della Ricerca Spaziale”initiative through the contract ASI N.2023-4-U.0.
文摘All-inorganic perovskites based on cesium-lead-bromine(Cs-Pb-Br)have been a prominent research focus in optoelectronics in recent years.The optimisation and tunability of their macroscopic properties exploit the conformational flexibility,resulting in various crystal structures.Varying synthesis parameters can yield distinct crystal structures from Cs,Pb,and Br precursors,and manually exploring the relationship between these synthesis parameters and the resulting crystal structure is both labour-intensive and time-consuming.Machine learning(ML)can rapidly uncover insights and drive discoveries in chemical synthesis with the support of data,significantly reducing both the cost and development cycle of materials.Here,we gathered synthesis parameters from published literature(220 synthesis runs)and implemented eight distinct ML models,including eXtreme Gradient Boosting(XGB),Decision Tree(DT),Support Vector Machine(SVM),Random Forest(RF),Naïve Bayes(NB),Logistic Regression(LR),Gradient Boosting(GB),and K-Nearest(KN)to classify and predict Cs-Pb-Br crystal structures from given synthesis parameters.Validation accuracy,precision,F1 score,recall,and average area under the curve(AUC)are employed to evaluate these ML models.The XGB model exhibited the best performance,achieving a validation accuracy of 0.841.The trained XGB model was subsequently utilised to predict the structure from 10 experimental runs using a randomised set of parameters,achieving a testing accuracy of 0.8.The results indicate that the Cs/Pb molar ratio,reaction time,and the concentration of organic compounds(ligands)play crucial roles in synthesising various crystal structures of Cs-Pb-Br.This study demonstrates a significant decrease in effort required for experimental procedures and builds a foundational basis for predicting crystal structures from synthesis parameters.
基金financially supported by the project of the National Observation and Research Station of the Institute of Geology,China Earthquake Administration(Grant No.NORSCBS22-06)the Youth Science and Technology Development Project of the Jilin Earthquake Agency(Grant No.JZQ-202402)+1 种基金the Earthquake Science and Technology Spark Project(Grant No.XH23013B)the China Scholarship Council(Grant No.202104190014)。
文摘Cenozoic trachytes are characteristic of some active volcanic fields in China.In particular,the origin and mechanisms of the evolution of trachytes from the Tianchi(Changbaishan)volcano(TV,China/North Korea)are poorly known.Here,we present new geochronological,geochemical and isotopic data on two trachytic suites outcropping on the northern and southern upper slopes of TV.Detailed zircon laser ablation-multicollector-inductively coupled plasma-mass spectrometry(LA-MC-ICP-MS)U-Pb dating,Rb-Sr isochron dating of plagioclase and hornblende,^(40)Ar/^(39)Ar chronology with mineral chemistry,whole-rock element and Sr-Nd-Pb isotope data are used to explore their origins and evolutionary mechanisms during the late Middle Pleistocene.Our data indicate that the trachytes mainly consist of sanidine,orthoclase and plagioclase,with minor albite,quartz and hornblende.They formed at 0.353-0.346 Ma(lava flow from the northern slope)and 0.383-0.311 Ma(lava flow from the southern slope),respectively.The TV trachytes are characterized by high K_(2)O/Na_(2)O and AR values,with low A/CNK and Mg~#values.They are enriched in rare earth elements(REEs;except Eu),depleted in Sr and Ba,crystallizing at 742-858℃.The TV trachytes have high(^(87)Sr/^(86)Sr)_i values(0.70776-0.71195),positiveε_(Nd)(t)values(0.61-2.93)and radiogenic^(206)Pb/^(204)Pb values(17.515-17.806).These values are similar to those of the Pleistocene and Holocene TV trachytes.Geochemical data indicate that they were formed by fractional crystallization from a basaltic melt and assimilated upper crust material.The trace element pattern of the studied trachytes is consistent with an evolution from basaltic melts representative of an enriched mantle source.The vent from which the trachytic magma was erupted probably collapsed inside the caldera during the TV'Millennium'eruption(ME)in 946 AD.The contemporaneous emission of basaltic and trachytic magma during the Middle-Late Pleistocene suggests the coexistence of fissural basaltic volcanism and central-type trachytic volcanism,the latter of which was associated with a magma chamber in the upper crust during the TV cone-construction stage.
文摘BACKGROUND Median sternotomy has been considered the gold standard approach for anterior mediastinal tumor resection.However,recent advances in video-assisted thoracoscopic surgery and robotic-assisted thoracoscopic surgery with carbon dioxide insufflation have allowed minimally invasive approaches even for large and locally invasive tumors of the upper-anterior mediastinum.The subxiphoid robotic optical approach is a recently developed technique for accessing the mediastinum.The trans-subxiphoid technique offers excellent exposure of the surgical field,reduces postoperative pain,facilitates specimen retrieval even for large tumors,and potentially improves early surgical outcomes.AIM To evaluate the safety,feasibility,and outcomes of a robotic subxiphoid approach for the resecting of large/invasive mediastinal tumors.METHODS Between July 2024 and September 2025,12 patients underwent subxiphoid robotic mediastinal resection.The diameter of the operated lesions ranged from 30 mm to 70 mm.A 3 cm subxiphoid incision was made at the subxiphoid level for GelPort placement,allowing for optical port access.Two operating ports were placed at the sixth intercostal space bilaterally.Carbon dioxide insufflations(8-10 mmHg)enlarged the surgical field,improving visualization of critical anatomical landmarks,such as the internal mammary arteries and phrenic nerves.This approach allowed complete resection of large or invasive tumors,preserving thoracic stability and reducing the risk of postoperative myasthenic crisis.RESULTS The mean operating time was 170.2 minutes,and the median hospital stay was 3.5 days.No major postoperative complications occurred.Two conversions were necessary:One with a lateral robotic approach due to previous abdominal surgery,and one with a sternotomy for tumor invasion of the aortic arch.Histopathological analysis identified nine thymomas and one solitary fibrous tumor.CONCLUSION Subxiphoid robotic approach is a safe,effective technique for extended thymectomy,fulfilling both oncological and myasthenia gravis surgical objectives.
