Objective:To evaluate the effect of allagic acid treatment on the cell viability of human prostate cancer cells.Methods:Ellagic acid(10-100 mol/L) treatment(48 h) of human prostate carcinoma PC3 cells was found to res...Objective:To evaluate the effect of allagic acid treatment on the cell viability of human prostate cancer cells.Methods:Ellagic acid(10-100 mol/L) treatment(48 h) of human prostate carcinoma PC3 cells was found to result in a dose-dependent inhibition of cell growth and apoptosis of PC3 cells as assessed by MTT assay,western blotting.flow cytometry and confocal microscopy.Results:We observed that ellagic acid treatment of PC3 cells resulted in a dose dependent inhibition of cell growth/cell viability.This ellagic acid caused cell growth inhibition was found to be accompanied by induction of apoptosis,as assessed by the cleavage of poly (ADP-ribose) polymerase(PARP) and morphological changes.Further,induction of apoptosis accompanied a decrease in the levels of antiapoptotic protein Bcl-2 and increase in proapoptotic protein Bax.thus shifting the Bax:Bcl-2 ratio in favor of apoptosis.Ellagic acid treatment of PC3 cells was also found to result in significant activation of caspases,as shown by the dose dependent decrease in the protein expression of procaspase-3,-6.-8 and -9.This ellagic acid-mediated induction of apoptosis was significantly(80%-90%) inhibited by the caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone(Z-VAD-FMK).Thus these data suggested an essential role of caspases in ellagic acid-mediated apoptosis of PC3 cells.Conclusions:It is tempting to suggest that consumption of tropical pigmented fruits and vegetables could be an effective strategy to combat prostate cancer.展开更多
Heart Failure(HF)is one of the leading problems in cardiology practice today.Acute decompensated heart failure(ADHF)is a significant cause of mortality and morbidity worldwide,and this is more relevant in the Asian su...Heart Failure(HF)is one of the leading problems in cardiology practice today.Acute decompensated heart failure(ADHF)is a significant cause of mortality and morbidity worldwide,and this is more relevant in the Asian subcontinent with a high population burden.Various regional registries in Asia have given us valuable insight into the aetiology and outcomes in this context.Though there are regional differences,it is clear from the review carried out in this paper that HF affects a much younger population.The comorbidities like diabetes and hypertension are high.Chronic kidney disease and atrial fibrillation are higher compared to the Western cohort.Heart failure with preserved ejection fraction is less often seen.The in-hospital mortality ranges from 2.8%to 8.4%.The one-year mortality is higher,and re-hospitalization is very high.Though in many Asian countries,guideline-directed medical therapy is initiated in hospitals,adherence is low.This is the challenge,and the way forward is only a practical team-based heart failure treatment approach.In the modern-day world of digital technology,the onus is on us to develop an integrated system of care in HF.This is by using available resources and opening up HF clinics.This review compares the strengths and limitations of various heart failure registries conducted in Asia and also gives a direction to plan effective strategies for improving heart failure care.展开更多
基金the College of Medicine Research Center (CMRC), Abha, College of Medicine, King Khalid University, Kingdom of Saudi Arabia for granting this study
文摘Objective:To evaluate the effect of allagic acid treatment on the cell viability of human prostate cancer cells.Methods:Ellagic acid(10-100 mol/L) treatment(48 h) of human prostate carcinoma PC3 cells was found to result in a dose-dependent inhibition of cell growth and apoptosis of PC3 cells as assessed by MTT assay,western blotting.flow cytometry and confocal microscopy.Results:We observed that ellagic acid treatment of PC3 cells resulted in a dose dependent inhibition of cell growth/cell viability.This ellagic acid caused cell growth inhibition was found to be accompanied by induction of apoptosis,as assessed by the cleavage of poly (ADP-ribose) polymerase(PARP) and morphological changes.Further,induction of apoptosis accompanied a decrease in the levels of antiapoptotic protein Bcl-2 and increase in proapoptotic protein Bax.thus shifting the Bax:Bcl-2 ratio in favor of apoptosis.Ellagic acid treatment of PC3 cells was also found to result in significant activation of caspases,as shown by the dose dependent decrease in the protein expression of procaspase-3,-6.-8 and -9.This ellagic acid-mediated induction of apoptosis was significantly(80%-90%) inhibited by the caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone(Z-VAD-FMK).Thus these data suggested an essential role of caspases in ellagic acid-mediated apoptosis of PC3 cells.Conclusions:It is tempting to suggest that consumption of tropical pigmented fruits and vegetables could be an effective strategy to combat prostate cancer.
文摘Heart Failure(HF)is one of the leading problems in cardiology practice today.Acute decompensated heart failure(ADHF)is a significant cause of mortality and morbidity worldwide,and this is more relevant in the Asian subcontinent with a high population burden.Various regional registries in Asia have given us valuable insight into the aetiology and outcomes in this context.Though there are regional differences,it is clear from the review carried out in this paper that HF affects a much younger population.The comorbidities like diabetes and hypertension are high.Chronic kidney disease and atrial fibrillation are higher compared to the Western cohort.Heart failure with preserved ejection fraction is less often seen.The in-hospital mortality ranges from 2.8%to 8.4%.The one-year mortality is higher,and re-hospitalization is very high.Though in many Asian countries,guideline-directed medical therapy is initiated in hospitals,adherence is low.This is the challenge,and the way forward is only a practical team-based heart failure treatment approach.In the modern-day world of digital technology,the onus is on us to develop an integrated system of care in HF.This is by using available resources and opening up HF clinics.This review compares the strengths and limitations of various heart failure registries conducted in Asia and also gives a direction to plan effective strategies for improving heart failure care.
