Recently gold nanoparticles(GNPs)have been actively studied as photot hermal converters,drug carriers,and imaging agents in a wide range of applications in cancer diagnosis and ther apy.The prolonged peroral administr...Recently gold nanoparticles(GNPs)have been actively studied as photot hermal converters,drug carriers,and imaging agents in a wide range of applications in cancer diagnosis and ther apy.The prolonged peroral administration of GNPs in a range of sizes was performed to investigate the morphological changes and their reversibility in the internal organs of laboratory animals.In this study,GNP's with average diameters of 2 nm,15 nm and 50nm were administered during 30 days,and the reversibility of morphological changes was investigated 14 days after administration.After the prolonged administration of GNPs,the severity of morphological changes in the liver,kidney,spleen and lymph nodes depended on the nanoparticle size.Specifically,50 nm nano-particles caused the most pronounced dystrophic and necrobiotic effects,whereas the smallest 2 nm nanoparticles caused proliferative changes.Most importantly,the development of patho-logical processes was reversible,as evidenced by the gradual restoration of the organ structure at 14 days after the end of GNPs administration.展开更多
Endoscopic ultrasound(EUS)is an indispensable tool for the diagnosis and management of various diseases,particularly biliopancreatic disorders,as it provides detailed visualization of the gastrointestinal tract and su...Endoscopic ultrasound(EUS)is an indispensable tool for the diagnosis and management of various diseases,particularly biliopancreatic disorders,as it provides detailed visualization of the gastrointestinal tract and surrounding structures.As the demand for diagnostic and interventional EUS procedures increases,ensuring high-quality training for endoscopists is essential to improve patient outcomes.This mini-review provides an overview of the current state of EUS training and emphasizes the importance of a structured approach that integrates theoretical knowledge and hands-on experience.We discuss different training methods,focusing on the main courses available worldwide,and highlight their advantages and limitations.In addition,we examine the challenges of training for diagnostic and interventional EUS,such as limited access to training centers and the need for personalized feedback.Overall,improving EUS training programs is essential to enhance physician skills and ensure this advanced technique is used safely and efficiently in clinical practice.展开更多
Digestive endoscopy is widely performed in clinical practice,including in children,and has revolutionized the diagnosis and treatment of many gastro-intestinal(GI)disorders.Interventional procedures are increasingly u...Digestive endoscopy is widely performed in clinical practice,including in children,and has revolutionized the diagnosis and treatment of many gastro-intestinal(GI)disorders.Interventional procedures are increasingly utilized,particularly for hepatobiliary and pancreatic diseases.However,only a limited number of gastroenterologists are trained and experienced to perform endoscopic retrograde cholangiopancreatography and endoscopic ultrasound in pediatric patients.While GI endoscopic emergencies in children are uncommon,they can be serious.Effective care demands true multidisciplinary teamwork,with close and ongoing collaboration between gastroenterologists,anesthetists,and the pe-diatric team especially in centres where pediatric endoscopy specialists are not available.This mini-review outlines current practices in pediatric digestive endoscopy and explores recent advances in interventional endoscopy compared to adult patients.展开更多
Endotracheal intubation-related complications are common in clinical,and there are currently no effective strategies to address these matters.Inspired by the biological characteristics of human airway mucus(HAM),an ar...Endotracheal intubation-related complications are common in clinical,and there are currently no effective strategies to address these matters.Inspired by the biological characteristics of human airway mucus(HAM),an artificial airway mucus(ARM)coating is straightforwardly constructed by combining carboxymethyl chitosan with methyl cellulose.The ARM coating exhibited excellent lubricity(coefficient of friction(Co F)=0.05)and hydrophilicity(water contact angle(WCA)=21.3°),and was capable of coating both the internal and external surfaces of the endotracheal tube(ETT).In vitro experiments demonstrated that the ARM coating not only showed good broad-spectrum antibacterial activity,but also significantly reduced nonspecific protein adhesion.Through an in vivo intubation cynomolgus monkey model,ARM-coated ETT potently mitigated airway injury and inflammation,and was highly potential to prevent bacterial infection and catheter blockage.This work offers a promising avenue for the development of airway-friendly invasive devices.展开更多
In the last three decades,the understanding of the renin angiotensin system(RAS)has been changed by the discoveries of functional local systems,novel biologically active peptides,additional specific receptors,alternat...In the last three decades,the understanding of the renin angiotensin system(RAS)has been changed by the discoveries of functional local systems,novel biologically active peptides,additional specific receptors,alternative pathways of angiotensin(Ang)?Ⅱ?generation,and new roles for enzymes and precursor components other than those in Ang?Ⅱ?synthesis.In this regard,the discovery that Ang-(1-7)opposes the pressor,proliferative,pro-fibrotic,and pro-inflammatory effects mediated by Ang?Ⅱ?has contributed to the realization that the RAS is composed of two axes.The first axis consists of the angiotensin-converting enzyme(ACE),with Ang?Ⅱ?as the end product,and the angiotensin type 1(AT1)receptor as the main effector mediating the biological actions of Ang?Ⅱ.The second axis results from ACE2-mediated hydrolysis of Ang?Ⅱ,leading to the production of Ang-(1-7),with the Mas receptor as the main effector conveying the vasodilatory,antiproliferative,anti-fibrotic,and anti-inflammatory effects of Ang-(1-7).Experimental and clinical studies have shown that both axes of the RAS may take part in the pathogenesis of liver diseases.In this manuscript,we summarize the current evidence regarding the role of RAS in hepatic cirrhosis and its complications,including hemodynamic changes and hepatorenal syndrome.The therapeutic potential of the modulation of RAS molecules in liver diseases is also discussed.展开更多
Autoimmune hepatitis (AIH) is a rare chronic inflammatory disease of the liver, which affects a group of patients who lost their immunological tolerance to antigens of the liver. It is clinically characterized by hype...Autoimmune hepatitis (AIH) is a rare chronic inflammatory disease of the liver, which affects a group of patients who lost their immunological tolerance to antigens of the liver. It is clinically characterized by hypergammaglobulinemia, elevated liver enzymes, presence of autoantibodies and histological changes. Although being rare in children, it represents a serious cause of chronic hepatic disease that can lead to cirrhosis and hepatic failure. Clinical findings, exclusion of more common liver disorders and the detection of antibodies antinuclear antibodies, smooth muscle antibodies and anti-LKM1 are usually enough for diagnosis on clinical practice. The pathogenic mechanisms that lead to AIH remain obscure, but some research findings suggest the participation of immunologic and genetic factors. It is not yet knew the triggering factor or factors that stimulate inflammatory response. Several mechanisms proposed partially explain the immunologic findings of AIH. The knowledge of immune factors evolved might result in better markers of prognosis and response to treatment. In this review, we aim to evaluate the findings of research about genetic and immune markers and their perspectives of application in clinical practice especially in pediatric population.展开更多
Nano-Ag incorporated hydroxyapatite/titania （HA/TiO2） coatings were deposited on Ti6A14V substrates by the plasma electrolytic oxidation process. Compared with the substrate, the deposited coatings display attractiv...Nano-Ag incorporated hydroxyapatite/titania （HA/TiO2） coatings were deposited on Ti6A14V substrates by the plasma electrolytic oxidation process. Compared with the substrate, the deposited coatings display attractive mechani- cal and biomedical properties. First, the coatings have stronger wear resistance and corrosion resistance. Second, they show a strong antibacterial ability. The mean vitality of the P. gingivalis on the coating surfaces is reduced to about 21%. Third, the coatings have good biocompatibility. The mean viability of the fibroblast cells on the coating surface is increased to about 130%. With these attractive properties, Ag incorporated HA/TiO2 coatings may be useful in the biomedical field.展开更多
Confocal Raman microspectroscopy(CRM)with 633-and 785-nm excitation wavelengths combined with optical clearing(OC)technique was used for ex-vivo study of porcine skin in the Raman fingerprint region.The optical cleari...Confocal Raman microspectroscopy(CRM)with 633-and 785-nm excitation wavelengths combined with optical clearing(OC)technique was used for ex-vivo study of porcine skin in the Raman fingerprint region.The optical clearing has been performed on the skin samples by applying a mixture of glycerol and distilled water and a mixture of glycerol,distilled water and chemical penetration enhancer dimethyl sulfoxide(DMSO)during 30 min and 60 min of treat-ment.It was shown that the combined use of the optical clearing technique and CRM at 633 nm allowed one to preserve the high probing depth,signal-to-noise ratio and spectral resolution simultaneously.Comparing the effect of different optical clearing agents on porcine skin showed that an optical clearing agent containing chemical penetration enhancer provides higher optical clearing efficiency.Also,an increase in treatment time allows to improve the optical clearing efficiency of both optical clearing agents.As a result of optical clearing,the detection of the amide-Ⅲ spectral region indicating well-distinguishable structural differences between the type-Ⅰ and type-Ⅳ collagens has been improved.展开更多
BACKGROUND Bipolar disorder(BD)is a severe psychiatric disorder characterized by mood swings.Psychosocial interventions,such as psychoeducation,play an essential role in promoting social rehabilitation and improving p...BACKGROUND Bipolar disorder(BD)is a severe psychiatric disorder characterized by mood swings.Psychosocial interventions,such as psychoeducation,play an essential role in promoting social rehabilitation and improving pharmacological treatment.AIM To investigate the role of psychoeducation in BD.METHODS A systematic review of original studies regarding psychoeducation interventions in patients with BD and their relatives was developed.A systematic literature search was performed using the Medline,Scopus,and Lilacs databases.No review articles or qualitative studies were included in the analysis.There were no date restriction criteria,and studies published up to April 2021 were included.RESULTS A total of forty-seven studies were selected for this review.Thirty-eight studies included patients,and nine included family members.Psychoeducation of patients and family members was associated with a lower number of new mood episodes and a reduction in number and length of stay of hospitalizations.Psychoeducational interventions with patients are associated with improved adherence to drug treatment.The strategies studied in patients and family members do not interfere with the severity of symptoms of mania or depression or with the patient's quality of life or functionality.Psychoeducational interventions with family members do not alter patients'adherence to pharmacotherapy.CONCLUSION Psychoeducation as an adjunct strategy to pharmacotherapy in the treatment of BD leads to a reduction in the frequency of new mood episodes,length of hospital stay and adherence to drug therapy.展开更多
Algorithms for reconstruction of linear and circular birefringence-dichroism of optically thin anisotropic biological layers are presented.The technique of Jones matrix tomography of poly-crystalline films of biologic...Algorithms for reconstruction of linear and circular birefringence-dichroism of optically thin anisotropic biological layers are presented.The technique of Jones matrix tomography of poly-crystalline films of biological fuids of various human organs has been developed and experimentally tested.The coordinate distributions of phase and amplitude anisotropy of bile films and synovial fuid taken from the knee joint are determined and statistically analyzed.Criteria(statistical moments of 3rd and 4th orders)of differential diagnostics of early stages of cholelithiasis and septic arthritis of the knee joint with excellent balanced accuracy were determined.Data on the diagnostic fficiency of the Jones matrix tomography method for polyerystalline plasma(liver disease),urine(albuminuria)and cytological smears(cervical cancer)are presented.展开更多
Optical immersion clearing is a technique that has been widely studied for more than two decades and that is used to originate a temporary transparency effect in biological tissues.If applied in cooperation with clini...Optical immersion clearing is a technique that has been widely studied for more than two decades and that is used to originate a temporary transparency effect in biological tissues.If applied in cooperation with clinical methods it provides optimization of diagnosis and treatment procedures.This technique turns biological tissues more transparent through two main mechanisms-tissue dehydration and refractive index(RI)matching between tissue compo-nents.Such matching is obtained by partial replacement of interstit ial water by a biocompat ible agent that presents higher RI and it can be completely rever sible by natural rehydration in vrivo or by assisted rehydration in ex vivo tissues.Experimental data to characterize and discriminate between the two mechanisms and to find new ones are necessary.Using a simple method,based on collimated transmittance and thickness measurements made from muscle samples under treatment,we have estimated the diffusion properties of glucose,ethylene glycol(EG)and water that were used to perform such char acterization and discrimination.Comparing these properties with data from literature that characterize their diffusion in water we have observed that musde cell membrane permeability limits agent and water diffusion in the muscle.The same experi-mental data has allowed to calculate the optical dearing(OC)eficiency and make an inter-pretation of the internal changes that occurred in muscle during the treatments.The same methodology can now be used to perform similar studies with other agents and in other tissues in order to solve engineering problems at design of inexpensive and robust technologies for a consider.able improvement of optical tomogr aphic techmiques with better contrast and in depth imaging.展开更多
At present,gold nanoparticles(GNPs)are widely used in biomedical applications such as cancer diagnostics and therapy.Accordingly,the potential toxicity hazards of these nanomaterials and human safety concerns are gain...At present,gold nanoparticles(GNPs)are widely used in biomedical applications such as cancer diagnostics and therapy.Accordingly,the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention.Here,we report the effects of prolonged peroral administration of GNPs with different sizes(2,15 and 50 nm)on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals.The experiment was conducted on 24 white mongrel male rats weighing 180–220 g,gold nanospheres sizes 2,15 and 50nm were administered orally for 15 days at a dosage of 190g/kg of animal body weight.The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability.The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood,especially pronounced after administration of GNPs with size of 50 nm.The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow.The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes,which were showed as the increasing number of immunoblasts and large lymphocytes.The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration,proliferation and di®erentiation of immune cells indicate the presence of immunostimulation effect of GNPs.展开更多
Despite more than 300 theories to explain the aging process, oxidative stress theory offers the best mechanism to explain aging and age related disorders. Several studies has shown the importance of oxidative stress d...Despite more than 300 theories to explain the aging process, oxidative stress theory offers the best mechanism to explain aging and age related disorders. Several studies has shown the importance of oxidative stress during aging. Pub Med, Science Direct and Springer online data bases are taken into consideration to write this mini-review. Human erythrocytes are most abundant and specialized cells in the body. Erythrocytes were extensively studied due to their metabolism and gas transport functions. Recent studies on erythrocytes have provided us detailed information of cell membrane and its structural organization that may help in studying the aging and age associated changes. The susceptibility of an organism is associated with the antioxidant potential of the body. Erythrocytes have potent antioxidant protection consisting of enzymatic and nonenzymatic pathways that counteract with reactive oxygen species, thus maintaining the redox regulation in the body. The non-enzymatic and enzymatic antioxidants and other biomarkers associated with erythrocyte membrane transport functions are the main content of this review. Biomarkers of oxidative stress in erythrocytes and its membrane were taken into the consideration during human aging that will be the main subject of this minireview.展开更多
This paper illustrates approaches to flooding risk governance in a territory where a high density of population corresponds to a high level of industrial activities and a significant development of transport infrastru...This paper illustrates approaches to flooding risk governance in a territory where a high density of population corresponds to a high level of industrial activities and a significant development of transport infrastructures in a transboundary context between France, Germany and Switzerland. In the last few years, some fundamental changes in European official instructions about risks and especially about flooding risks occurred. Substantial public policy challenges remain in order to promote a broad-based resilient territory twinned with local knowledge of sustainable land management and civic participation in local governance. The survey is based on a GIS study and questionnaires with a representative panel of 235 stakeholders from the studied territory. Our statistical results demonstrate that, in spite of a deep-rooted mindset as regards flooding hazards, local population awareness of the territorial vulnerability is not related to important challenges. A hierarchical analysis of the data shows that the awareness of stakeholders is less related to the importance of risks when the territorial level decreases. It also highlights the decline of solidarity among neighbouring communities when the distance from the industrial axis increases. Greatly impacted by flooding risks, industrial activities of the regional basin are paradoxically not really perceived as common challenges. Such a case study illustrates how public policies need to be relevant to the adapted levels. Awareness of common issues is a central target to develop acceptance in order to provide an efficient framework of resilience, including willingness to pay to reduce the risk and solidarity in local development compensations.展开更多
While it is known that the brain perceives color and motion asynchronously, the specific locations in which the brain binds signals remain unknown. This study distinguishes subjective perception of the capability to b...While it is known that the brain perceives color and motion asynchronously, the specific locations in which the brain binds signals remain unknown. This study distinguishes subjective perception of the capability to bind features and the objective accuracy in feature binding. The stimuli were the same for individual subjects, consisting of random dots (red and green, or yellow and blue) moving either vertically or horizontally. Subjects responded to questions regarding the color or the direction of motion of the dots (objective judgment) and rated their capability in performing the task (subjective judgment). The imaging results of contrasting subjective judgment showed that the activation of the anterior rostral cingulate cortex (rACC) and inferior frontal gyrus (Brodmann area [BA] 45/47) during incapable-of-binding responses, compared with the capable-of-binding responses. It is suggested that the rACC is for uncertainty of subjective judgment and BA 45/47 is for the increased burden on working memory. In contrast, there was no imaging results of contrasting the correct and incorrect responses (i.e., objective judgment), and neither was there for the interaction between subjective and objective judgment. The results of conservative conjunction analysis indicated common and shared brain areas for the 2 distinctive binding situations (the correct and capable-of-binding vs the incorrect and incapable-of-binding), including increased activity in the intraparietal lobe (IPL) and the junction areas of the posterior rostral ACC (dACC) and the prefrontal areas, but decreased activity in the medial portion of the IPL, suggesting that feature binding requires maintaining attention. These results clearly isolated subjective judgment from objective judgment and support the view that maintaining attention is involved in feature binding of color and motion.展开更多
Porous(polyamide/hydroxyapatite)composites were manufactured via SLS(selective laser sintering)process.Specimens with different PA2200/HA contents(100/0;95/5;90/10 and 80/20)were sintered at relative low laser energy ...Porous(polyamide/hydroxyapatite)composites were manufactured via SLS(selective laser sintering)process.Specimens with different PA2200/HA contents(100/0;95/5;90/10 and 80/20)were sintered at relative low laser energy density.The porous composite specimens were characterized for dynamic-mechanical analysis.The dynamic-mechanical properties changed as a function of the composition of the composite materials.Storage and loss modulus vary from 1,050 to 215 MPa and 35 to 5 MPa,respectively.展开更多
Besides graphite,diamond,graphene,carbon nanotubes,and fullerenes,there is another allotrope of carbon,carbyne,existing in the form of a one-dimensional chain of carbon atoms.It has been theoretically predicted that c...Besides graphite,diamond,graphene,carbon nanotubes,and fullerenes,there is another allotrope of carbon,carbyne,existing in the form of a one-dimensional chain of carbon atoms.It has been theoretically predicted that carbyne would be stronger,stiffer,and more exotic than other materials that have been synthesized before.In this article,two kinds of carbyne,i.e.,cumulene and polyyne are investigated by the first principles,where the mechanical properties,electronic structure,optical and phonon properties of the carbynes are calculated.The results on the crystal binding energy and the formation energy show that though both are difficult to be synthesized from diamond or graphite,polyyne is more stable and harder than cummulene.The tensile stiffness,bond stiffness,and Young's modulus of cumulene are 94.669 eV/Å,90.334 GPa,and 60.62 GPa,respectively,while the corresponding values of polyyne are 94.939 eV/Å,101.42 GPa,and60.06 GPa.The supercell calculation shows that carbyne is most stable at N=5,where N is the supercell number,which indicates that the carbon chain with 10 atoms is most stable.The calculation on the electronic band structure shows that cumulene is a conductor and polyyne is a semiconductor with a band gap of 0.37 eV.The dielectric function of carbynes varies along different directions,consistent with the one-dimensional nature of the carbon chains.In the phonon dispersion of cumulene,there are imaginary frequencies with the lowest value down to-3.817 THz,which indicates that cumulene could be unstable at room temperature and normal pressure.展开更多
Objective: Verify if the educational technology based on Just in Time Education is relevant to Permanent Health Education in Workers’ Health. Method: This study is exploratory and descriptive qualitative research. We...Objective: Verify if the educational technology based on Just in Time Education is relevant to Permanent Health Education in Workers’ Health. Method: This study is exploratory and descriptive qualitative research. Were performed semi-structured interviews were carried out with the worker’s health team of a large company from the South of Brazil region. The collected data were carried out in 2021, between January and May, through the virtual environment, with eight participants from the Work Health Team of Eletrosul, located in Florianópolis, who agreed to participate in the research by signing the Free and Informed Consent Term, in accordance with the data saturation criterion. The research was approved by the Ethics Committee (n. 4079133) and the data were submitted to Bardin for thematic content analysis. Result: The results are presented from three categories: 1) Expansion and inclusion in Permanent Education needs;2) Tools adopted in educational processes;3) Just in Time Education: educational technology for workers’ health. Conclusion: The active methodology Just in Time Education helps the processes of Permanent Education in Health, which moved from a face-to-face model to e-learning and required the knowledge renewal in the technology area to maintain efficient communication. This methodology allows quick and accurate access to information from a reliable, adequate, and up-to-date source, causing a positive impact on the qualified and problem-solving work process, in line with the needs of the employer and the company. Therefore, Just in Time Education can provide standardization, organization, and informational and operational quality necessary in this context, in addition to serving as an indicator and data generator (management tool).展开更多
The characteristic of flow and heat transfer of A12Oa-water nanofluids flowing through a horizontal ventilated cavity is investigated numerically. The bottom wall is subjected to a linearly varying increasing heating ...The characteristic of flow and heat transfer of A12Oa-water nanofluids flowing through a horizontal ventilated cavity is investigated numerically. The bottom wall is subjected to a linearly varying increasing heating temperature profile, whereas the other boundaries are assumed to be thermally insulated. The enclosure is cooled by an injected or sucked imposed flow. The simulations are focused specifically on the effects of different key parameters such as Reynolds number, 200 〈 Re 〈 5,000, nanoparticles concentration, 0 〈 φ 〈 0.1, and mode of imposed flow, on the flow and thermal patterns and heat transfer performances. The findings demonstrate clearly the positive role of the nanoparticles addition on the improvement of the heat transfer rate and the mean temperature within the cavity. Also, the results presented show that, the suction mode is more favorable to the heat transfer in comparison with the case of the injection mode. The cooling efficiency is found to be more pronounced by applying the suction mode.展开更多
Objective: To evaluate the prevalence and determinants of overall cardiovascular risk among the pygmies of Congo Brazzaville. Methods: Cross-analysis of anthropometric data, clinical and laboratory 273 indigenous subj...Objective: To evaluate the prevalence and determinants of overall cardiovascular risk among the pygmies of Congo Brazzaville. Methods: Cross-analysis of anthropometric data, clinical and laboratory 273 indigenous subjects including 54 patients with cardiovascular disease (CVD) and 183 without CVD, aged 29 to 69 years. The cardiovascular risk stratification table WHO/ISH 2003 was used to assess overall cardiovascular risk level. Multiple logistic regression was used to assess the independent determinants of cardiovascular risk using the global left ventricular hypertrophy as a marker. Results: Fifty-four subjects had cardiovascular disease (CVD), including 31 women (36.7%). They were aged 51.9 ± 12.4 years (p < 0.05) and showed a high blood pressure in short-long (100 ± 77 vs 32 ± 48 months;p < 0.001). It was also found a pulse pressure, PPO (58 ± 8 vs 51 ± 4 mmHg). In multivariate analysis, HDL-C levels, fasting glucose, age greater than 50 years and the higher parity or equalizes to 5 among women have emerged as key determinants of CVD risk. Conclusion: Whatever the stage, arterial hypertension in this study is associated at the risk of cardiovascular diseases (CVD) high at the Pygmies of Congo, emphasizing the need for a more aggressive follow-up strategy.展开更多
基金supported by Grant No.14-13-01167 from the Russian Scientic Foundation.The morphological studies done by GNM,SSP,OVZ,IOB and NAN were conducted by the state task of Russian ministry of Health.The work done by VVT on interpretation of experimental data was supported by Grant No.14-15-00186 of the Russian Science Foundation.
文摘Recently gold nanoparticles(GNPs)have been actively studied as photot hermal converters,drug carriers,and imaging agents in a wide range of applications in cancer diagnosis and ther apy.The prolonged peroral administration of GNPs in a range of sizes was performed to investigate the morphological changes and their reversibility in the internal organs of laboratory animals.In this study,GNP's with average diameters of 2 nm,15 nm and 50nm were administered during 30 days,and the reversibility of morphological changes was investigated 14 days after administration.After the prolonged administration of GNPs,the severity of morphological changes in the liver,kidney,spleen and lymph nodes depended on the nanoparticle size.Specifically,50 nm nano-particles caused the most pronounced dystrophic and necrobiotic effects,whereas the smallest 2 nm nanoparticles caused proliferative changes.Most importantly,the development of patho-logical processes was reversible,as evidenced by the gradual restoration of the organ structure at 14 days after the end of GNPs administration.
文摘Endoscopic ultrasound(EUS)is an indispensable tool for the diagnosis and management of various diseases,particularly biliopancreatic disorders,as it provides detailed visualization of the gastrointestinal tract and surrounding structures.As the demand for diagnostic and interventional EUS procedures increases,ensuring high-quality training for endoscopists is essential to improve patient outcomes.This mini-review provides an overview of the current state of EUS training and emphasizes the importance of a structured approach that integrates theoretical knowledge and hands-on experience.We discuss different training methods,focusing on the main courses available worldwide,and highlight their advantages and limitations.In addition,we examine the challenges of training for diagnostic and interventional EUS,such as limited access to training centers and the need for personalized feedback.Overall,improving EUS training programs is essential to enhance physician skills and ensure this advanced technique is used safely and efficiently in clinical practice.
文摘Digestive endoscopy is widely performed in clinical practice,including in children,and has revolutionized the diagnosis and treatment of many gastro-intestinal(GI)disorders.Interventional procedures are increasingly utilized,particularly for hepatobiliary and pancreatic diseases.However,only a limited number of gastroenterologists are trained and experienced to perform endoscopic retrograde cholangiopancreatography and endoscopic ultrasound in pediatric patients.While GI endoscopic emergencies in children are uncommon,they can be serious.Effective care demands true multidisciplinary teamwork,with close and ongoing collaboration between gastroenterologists,anesthetists,and the pe-diatric team especially in centres where pediatric endoscopy specialists are not available.This mini-review outlines current practices in pediatric digestive endoscopy and explores recent advances in interventional endoscopy compared to adult patients.
基金supported by the National Natural Science Foundation of China(Nos.52203046 and 82171219)Sichuan Science and Technology Program(No.2023NSFSC1944)+3 种基金West China Nursing Discipline Development Special Fund ProjectSichuan University(No.HXHL21007)the China Postdoctoral Science Foundation(No.2023M742483)the National Natural Science Foundation of Guangdong(No.2024A1515012881)。
文摘Endotracheal intubation-related complications are common in clinical,and there are currently no effective strategies to address these matters.Inspired by the biological characteristics of human airway mucus(HAM),an artificial airway mucus(ARM)coating is straightforwardly constructed by combining carboxymethyl chitosan with methyl cellulose.The ARM coating exhibited excellent lubricity(coefficient of friction(Co F)=0.05)and hydrophilicity(water contact angle(WCA)=21.3°),and was capable of coating both the internal and external surfaces of the endotracheal tube(ETT).In vitro experiments demonstrated that the ARM coating not only showed good broad-spectrum antibacterial activity,but also significantly reduced nonspecific protein adhesion.Through an in vivo intubation cynomolgus monkey model,ARM-coated ETT potently mitigated airway injury and inflammation,and was highly potential to prevent bacterial infection and catheter blockage.This work offers a promising avenue for the development of airway-friendly invasive devices.
基金Supported by CNPq,No.460334/2014-0 and FAPEMIG,No.CDS-PPM-00555-15
文摘In the last three decades,the understanding of the renin angiotensin system(RAS)has been changed by the discoveries of functional local systems,novel biologically active peptides,additional specific receptors,alternative pathways of angiotensin(Ang)?Ⅱ?generation,and new roles for enzymes and precursor components other than those in Ang?Ⅱ?synthesis.In this regard,the discovery that Ang-(1-7)opposes the pressor,proliferative,pro-fibrotic,and pro-inflammatory effects mediated by Ang?Ⅱ?has contributed to the realization that the RAS is composed of two axes.The first axis consists of the angiotensin-converting enzyme(ACE),with Ang?Ⅱ?as the end product,and the angiotensin type 1(AT1)receptor as the main effector mediating the biological actions of Ang?Ⅱ.The second axis results from ACE2-mediated hydrolysis of Ang?Ⅱ,leading to the production of Ang-(1-7),with the Mas receptor as the main effector conveying the vasodilatory,antiproliferative,anti-fibrotic,and anti-inflammatory effects of Ang-(1-7).Experimental and clinical studies have shown that both axes of the RAS may take part in the pathogenesis of liver diseases.In this manuscript,we summarize the current evidence regarding the role of RAS in hepatic cirrhosis and its complications,including hemodynamic changes and hepatorenal syndrome.The therapeutic potential of the modulation of RAS molecules in liver diseases is also discussed.
基金Supported by CAPES, INCT-MM (FAPEMIG: CBB-APQ- 00075-09/CNPq 573646/2008-2)
文摘Autoimmune hepatitis (AIH) is a rare chronic inflammatory disease of the liver, which affects a group of patients who lost their immunological tolerance to antigens of the liver. It is clinically characterized by hypergammaglobulinemia, elevated liver enzymes, presence of autoantibodies and histological changes. Although being rare in children, it represents a serious cause of chronic hepatic disease that can lead to cirrhosis and hepatic failure. Clinical findings, exclusion of more common liver disorders and the detection of antibodies antinuclear antibodies, smooth muscle antibodies and anti-LKM1 are usually enough for diagnosis on clinical practice. The pathogenic mechanisms that lead to AIH remain obscure, but some research findings suggest the participation of immunologic and genetic factors. It is not yet knew the triggering factor or factors that stimulate inflammatory response. Several mechanisms proposed partially explain the immunologic findings of AIH. The knowledge of immune factors evolved might result in better markers of prognosis and response to treatment. In this review, we aim to evaluate the findings of research about genetic and immune markers and their perspectives of application in clinical practice especially in pediatric population.
基金Project supported by the Young Scientists Fund of the National Natural Science Foundation of China(Grant No.11005151)the National Natural Science Foundation of China(Grant No.11347110)+2 种基金the Open Research Fund of the Key Lab of Broadband Wireless Communication and Sensor Network Technology of Ministry of Education,Nanjing University of Posts and Telecommunications,China(Grant No.NYKL201303)the Scientific Research Foundation of Nanjing University of Posts and Telecommunications,China(Grant No.NY213054)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘Nano-Ag incorporated hydroxyapatite/titania （HA/TiO2） coatings were deposited on Ti6A14V substrates by the plasma electrolytic oxidation process. Compared with the substrate, the deposited coatings display attractive mechani- cal and biomedical properties. First, the coatings have stronger wear resistance and corrosion resistance. Second, they show a strong antibacterial ability. The mean vitality of the P. gingivalis on the coating surfaces is reduced to about 21%. Third, the coatings have good biocompatibility. The mean viability of the fibroblast cells on the coating surface is increased to about 130%. With these attractive properties, Ag incorporated HA/TiO2 coatings may be useful in the biomedical field.
基金Ths work was supported by the VEKOP-2.3.2-16-2016-00011 Grantwhich is co-financed by the European Union and European Social FundValery V.Tuchin was supported by RFBR Grant 18-52-16025 and the Grant of the Government of the Russian Federation(Registration No.2020-220-08-2389).
文摘Confocal Raman microspectroscopy(CRM)with 633-and 785-nm excitation wavelengths combined with optical clearing(OC)technique was used for ex-vivo study of porcine skin in the Raman fingerprint region.The optical clearing has been performed on the skin samples by applying a mixture of glycerol and distilled water and a mixture of glycerol,distilled water and chemical penetration enhancer dimethyl sulfoxide(DMSO)during 30 min and 60 min of treat-ment.It was shown that the combined use of the optical clearing technique and CRM at 633 nm allowed one to preserve the high probing depth,signal-to-noise ratio and spectral resolution simultaneously.Comparing the effect of different optical clearing agents on porcine skin showed that an optical clearing agent containing chemical penetration enhancer provides higher optical clearing efficiency.Also,an increase in treatment time allows to improve the optical clearing efficiency of both optical clearing agents.As a result of optical clearing,the detection of the amide-Ⅲ spectral region indicating well-distinguishable structural differences between the type-Ⅰ and type-Ⅳ collagens has been improved.
文摘BACKGROUND Bipolar disorder(BD)is a severe psychiatric disorder characterized by mood swings.Psychosocial interventions,such as psychoeducation,play an essential role in promoting social rehabilitation and improving pharmacological treatment.AIM To investigate the role of psychoeducation in BD.METHODS A systematic review of original studies regarding psychoeducation interventions in patients with BD and their relatives was developed.A systematic literature search was performed using the Medline,Scopus,and Lilacs databases.No review articles or qualitative studies were included in the analysis.There were no date restriction criteria,and studies published up to April 2021 were included.RESULTS A total of forty-seven studies were selected for this review.Thirty-eight studies included patients,and nine included family members.Psychoeducation of patients and family members was associated with a lower number of new mood episodes and a reduction in number and length of stay of hospitalizations.Psychoeducational interventions with patients are associated with improved adherence to drug treatment.The strategies studied in patients and family members do not interfere with the severity of symptoms of mania or depression or with the patient's quality of life or functionality.Psychoeducational interventions with family members do not alter patients'adherence to pharmacotherapy.CONCLUSION Psychoeducation as an adjunct strategy to pharmacotherapy in the treatment of BD leads to a reduction in the frequency of new mood episodes,length of hospital stay and adherence to drug therapy.
基金support of the Academy of Finland(grant projects:311698)support of INFOTECH grant project,as well as the MEPhI Academic Excellence Project(Contract No.02.a03.21.0005)the National Research Tomsk State University Academic D.I.Mendeleev Fund Program.
文摘Algorithms for reconstruction of linear and circular birefringence-dichroism of optically thin anisotropic biological layers are presented.The technique of Jones matrix tomography of poly-crystalline films of biological fuids of various human organs has been developed and experimentally tested.The coordinate distributions of phase and amplitude anisotropy of bile films and synovial fuid taken from the knee joint are determined and statistically analyzed.Criteria(statistical moments of 3rd and 4th orders)of differential diagnostics of early stages of cholelithiasis and septic arthritis of the knee joint with excellent balanced accuracy were determined.Data on the diagnostic fficiency of the Jones matrix tomography method for polyerystalline plasma(liver disease),urine(albuminuria)and cytological smears(cervical cancer)are presented.
基金support from the Government of Russian Federation(Grant No.14.Z50.31.0004)The Tomsk State University Academic D.I.Mendeleev Fund Program.
文摘Optical immersion clearing is a technique that has been widely studied for more than two decades and that is used to originate a temporary transparency effect in biological tissues.If applied in cooperation with clinical methods it provides optimization of diagnosis and treatment procedures.This technique turns biological tissues more transparent through two main mechanisms-tissue dehydration and refractive index(RI)matching between tissue compo-nents.Such matching is obtained by partial replacement of interstit ial water by a biocompat ible agent that presents higher RI and it can be completely rever sible by natural rehydration in vrivo or by assisted rehydration in ex vivo tissues.Experimental data to characterize and discriminate between the two mechanisms and to find new ones are necessary.Using a simple method,based on collimated transmittance and thickness measurements made from muscle samples under treatment,we have estimated the diffusion properties of glucose,ethylene glycol(EG)and water that were used to perform such char acterization and discrimination.Comparing these properties with data from literature that characterize their diffusion in water we have observed that musde cell membrane permeability limits agent and water diffusion in the muscle.The same experi-mental data has allowed to calculate the optical dearing(OC)eficiency and make an inter-pretation of the internal changes that occurred in muscle during the treatments.The same methodology can now be used to perform similar studies with other agents and in other tissues in order to solve engineering problems at design of inexpensive and robust technologies for a consider.able improvement of optical tomogr aphic techmiques with better contrast and in depth imaging.
文摘At present,gold nanoparticles(GNPs)are widely used in biomedical applications such as cancer diagnostics and therapy.Accordingly,the potential toxicity hazards of these nanomaterials and human safety concerns are gaining significant attention.Here,we report the effects of prolonged peroral administration of GNPs with different sizes(2,15 and 50 nm)on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals.The experiment was conducted on 24 white mongrel male rats weighing 180–220 g,gold nanospheres sizes 2,15 and 50nm were administered orally for 15 days at a dosage of 190g/kg of animal body weight.The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability.The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood,especially pronounced after administration of GNPs with size of 50 nm.The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow.The signs of strengthening of the processes of differentiation and maturation of cellular elements were found in lymph nodes,which were showed as the increasing number of immunoblasts and large lymphocytes.The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration,proliferation and di®erentiation of immune cells indicate the presence of immunostimulation effect of GNPs.
基金Fellowship(to Pawan Kumar Maurya)(Science without Borders-Level A)from coordination of Improvement of Higher Education Personnel(CAPES),National Counsel of Technological and Scientific Development(CNPq),Brazil
文摘Despite more than 300 theories to explain the aging process, oxidative stress theory offers the best mechanism to explain aging and age related disorders. Several studies has shown the importance of oxidative stress during aging. Pub Med, Science Direct and Springer online data bases are taken into consideration to write this mini-review. Human erythrocytes are most abundant and specialized cells in the body. Erythrocytes were extensively studied due to their metabolism and gas transport functions. Recent studies on erythrocytes have provided us detailed information of cell membrane and its structural organization that may help in studying the aging and age associated changes. The susceptibility of an organism is associated with the antioxidant potential of the body. Erythrocytes have potent antioxidant protection consisting of enzymatic and nonenzymatic pathways that counteract with reactive oxygen species, thus maintaining the redox regulation in the body. The non-enzymatic and enzymatic antioxidants and other biomarkers associated with erythrocyte membrane transport functions are the main content of this review. Biomarkers of oxidative stress in erythrocytes and its membrane were taken into the consideration during human aging that will be the main subject of this minireview.
文摘This paper illustrates approaches to flooding risk governance in a territory where a high density of population corresponds to a high level of industrial activities and a significant development of transport infrastructures in a transboundary context between France, Germany and Switzerland. In the last few years, some fundamental changes in European official instructions about risks and especially about flooding risks occurred. Substantial public policy challenges remain in order to promote a broad-based resilient territory twinned with local knowledge of sustainable land management and civic participation in local governance. The survey is based on a GIS study and questionnaires with a representative panel of 235 stakeholders from the studied territory. Our statistical results demonstrate that, in spite of a deep-rooted mindset as regards flooding hazards, local population awareness of the territorial vulnerability is not related to important challenges. A hierarchical analysis of the data shows that the awareness of stakeholders is less related to the importance of risks when the territorial level decreases. It also highlights the decline of solidarity among neighbouring communities when the distance from the industrial axis increases. Greatly impacted by flooding risks, industrial activities of the regional basin are paradoxically not really perceived as common challenges. Such a case study illustrates how public policies need to be relevant to the adapted levels. Awareness of common issues is a central target to develop acceptance in order to provide an efficient framework of resilience, including willingness to pay to reduce the risk and solidarity in local development compensations.
文摘While it is known that the brain perceives color and motion asynchronously, the specific locations in which the brain binds signals remain unknown. This study distinguishes subjective perception of the capability to bind features and the objective accuracy in feature binding. The stimuli were the same for individual subjects, consisting of random dots (red and green, or yellow and blue) moving either vertically or horizontally. Subjects responded to questions regarding the color or the direction of motion of the dots (objective judgment) and rated their capability in performing the task (subjective judgment). The imaging results of contrasting subjective judgment showed that the activation of the anterior rostral cingulate cortex (rACC) and inferior frontal gyrus (Brodmann area [BA] 45/47) during incapable-of-binding responses, compared with the capable-of-binding responses. It is suggested that the rACC is for uncertainty of subjective judgment and BA 45/47 is for the increased burden on working memory. In contrast, there was no imaging results of contrasting the correct and incorrect responses (i.e., objective judgment), and neither was there for the interaction between subjective and objective judgment. The results of conservative conjunction analysis indicated common and shared brain areas for the 2 distinctive binding situations (the correct and capable-of-binding vs the incorrect and incapable-of-binding), including increased activity in the intraparietal lobe (IPL) and the junction areas of the posterior rostral ACC (dACC) and the prefrontal areas, but decreased activity in the medial portion of the IPL, suggesting that feature binding requires maintaining attention. These results clearly isolated subjective judgment from objective judgment and support the view that maintaining attention is involved in feature binding of color and motion.
文摘Porous(polyamide/hydroxyapatite)composites were manufactured via SLS(selective laser sintering)process.Specimens with different PA2200/HA contents(100/0;95/5;90/10 and 80/20)were sintered at relative low laser energy density.The porous composite specimens were characterized for dynamic-mechanical analysis.The dynamic-mechanical properties changed as a function of the composition of the composite materials.Storage and loss modulus vary from 1,050 to 215 MPa and 35 to 5 MPa,respectively.
基金Project supported by the National Natural Science Foundation of China(Grant No.11672087)the Strategic Programs for Innovative Research(SPIRE)+1 种基金the Computational Materials Science Initiative(CMSI)the Yukawa International Program for Quark-Hadron Sciences at YITP,Kyoto University,Japan
文摘Besides graphite,diamond,graphene,carbon nanotubes,and fullerenes,there is another allotrope of carbon,carbyne,existing in the form of a one-dimensional chain of carbon atoms.It has been theoretically predicted that carbyne would be stronger,stiffer,and more exotic than other materials that have been synthesized before.In this article,two kinds of carbyne,i.e.,cumulene and polyyne are investigated by the first principles,where the mechanical properties,electronic structure,optical and phonon properties of the carbynes are calculated.The results on the crystal binding energy and the formation energy show that though both are difficult to be synthesized from diamond or graphite,polyyne is more stable and harder than cummulene.The tensile stiffness,bond stiffness,and Young's modulus of cumulene are 94.669 eV/Å,90.334 GPa,and 60.62 GPa,respectively,while the corresponding values of polyyne are 94.939 eV/Å,101.42 GPa,and60.06 GPa.The supercell calculation shows that carbyne is most stable at N=5,where N is the supercell number,which indicates that the carbon chain with 10 atoms is most stable.The calculation on the electronic band structure shows that cumulene is a conductor and polyyne is a semiconductor with a band gap of 0.37 eV.The dielectric function of carbynes varies along different directions,consistent with the one-dimensional nature of the carbon chains.In the phonon dispersion of cumulene,there are imaginary frequencies with the lowest value down to-3.817 THz,which indicates that cumulene could be unstable at room temperature and normal pressure.
文摘Objective: Verify if the educational technology based on Just in Time Education is relevant to Permanent Health Education in Workers’ Health. Method: This study is exploratory and descriptive qualitative research. Were performed semi-structured interviews were carried out with the worker’s health team of a large company from the South of Brazil region. The collected data were carried out in 2021, between January and May, through the virtual environment, with eight participants from the Work Health Team of Eletrosul, located in Florianópolis, who agreed to participate in the research by signing the Free and Informed Consent Term, in accordance with the data saturation criterion. The research was approved by the Ethics Committee (n. 4079133) and the data were submitted to Bardin for thematic content analysis. Result: The results are presented from three categories: 1) Expansion and inclusion in Permanent Education needs;2) Tools adopted in educational processes;3) Just in Time Education: educational technology for workers’ health. Conclusion: The active methodology Just in Time Education helps the processes of Permanent Education in Health, which moved from a face-to-face model to e-learning and required the knowledge renewal in the technology area to maintain efficient communication. This methodology allows quick and accurate access to information from a reliable, adequate, and up-to-date source, causing a positive impact on the qualified and problem-solving work process, in line with the needs of the employer and the company. Therefore, Just in Time Education can provide standardization, organization, and informational and operational quality necessary in this context, in addition to serving as an indicator and data generator (management tool).
文摘The characteristic of flow and heat transfer of A12Oa-water nanofluids flowing through a horizontal ventilated cavity is investigated numerically. The bottom wall is subjected to a linearly varying increasing heating temperature profile, whereas the other boundaries are assumed to be thermally insulated. The enclosure is cooled by an injected or sucked imposed flow. The simulations are focused specifically on the effects of different key parameters such as Reynolds number, 200 〈 Re 〈 5,000, nanoparticles concentration, 0 〈 φ 〈 0.1, and mode of imposed flow, on the flow and thermal patterns and heat transfer performances. The findings demonstrate clearly the positive role of the nanoparticles addition on the improvement of the heat transfer rate and the mean temperature within the cavity. Also, the results presented show that, the suction mode is more favorable to the heat transfer in comparison with the case of the injection mode. The cooling efficiency is found to be more pronounced by applying the suction mode.
文摘Objective: To evaluate the prevalence and determinants of overall cardiovascular risk among the pygmies of Congo Brazzaville. Methods: Cross-analysis of anthropometric data, clinical and laboratory 273 indigenous subjects including 54 patients with cardiovascular disease (CVD) and 183 without CVD, aged 29 to 69 years. The cardiovascular risk stratification table WHO/ISH 2003 was used to assess overall cardiovascular risk level. Multiple logistic regression was used to assess the independent determinants of cardiovascular risk using the global left ventricular hypertrophy as a marker. Results: Fifty-four subjects had cardiovascular disease (CVD), including 31 women (36.7%). They were aged 51.9 ± 12.4 years (p < 0.05) and showed a high blood pressure in short-long (100 ± 77 vs 32 ± 48 months;p < 0.001). It was also found a pulse pressure, PPO (58 ± 8 vs 51 ± 4 mmHg). In multivariate analysis, HDL-C levels, fasting glucose, age greater than 50 years and the higher parity or equalizes to 5 among women have emerged as key determinants of CVD risk. Conclusion: Whatever the stage, arterial hypertension in this study is associated at the risk of cardiovascular diseases (CVD) high at the Pygmies of Congo, emphasizing the need for a more aggressive follow-up strategy.
