AIM: To investigate the effects of herbal compound 861 (Cpd 861) on cell proliferation in human hepatic stellate cells (LX-2) and human hepatocellular liver carcinoma cells (HepG2), and expression of α-smooth muscle ...AIM: To investigate the effects of herbal compound 861 (Cpd 861) on cell proliferation in human hepatic stellate cells (LX-2) and human hepatocellular liver carcinoma cells (HepG2), and expression of α-smooth muscle actin (α-SMA) in LX-2 cells.METHODS: LX-2 and HepG2 cells were incubated with various concentrations of Cpd 861 (0.1-0.003 mg/mL)for 1, 2, 3, 5 and 7 d. Cell proliferation was analyzed by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Effects of Cpd861on the expression of cc-SMA mRNA in LX-2 cells weremeasured by real-time quantitative PCR method using SYBRGreen I technology.RESULTS: Cpd 861, at 0.1 mg/mL, significantly inhibitedLX-2 cell proliferation (15% decrease relative to control,P<0.05) after 3 d of incubation. The inhibitory effects seemedto increase with the treatment time (25% decrease after5 d of incubation and 35% decrease after 7 d of incubation,P<0.01). However, Cpd 861 did not affect HepG2 cellproliferation at the same concentration used for I_X-2 cells.The expression levels of ^-SMA mRNA decreased significantlywhen LX-2 cells were exposed to Cpd 861 for 48 h (59%decrease relative to control, P<0.05) or 72 h (60% decreaserelative to control, P<0.01).CONCLUSION: Cpd 861 can significantly inhibit LX-2 cellproliferation in a dose-dependant manner, and reduce theexpression levels of o^-SMA mRNA in LX-2 cells. Since hepaticcell proliferation and high level of ^-SMA are associatedwith liver fibrosis, the results suggest that Cpd 861 may beuseful in the treatment of this disease.展开更多
A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically opt...A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, concentration of running buffers, addition of sulfated b-CD, applied voltage and column temperature. Baseline separation was achieved for the nine xanthones in less than 15 minutes using a background electrolyte consisting of 200 mmol/L borate (pH 9.5) and 10 mmol/L sulfated b-CD.展开更多
Grifola umbellata (Pers.) Pilat is a fungus whose sclerotium has potent pharmaceutical values.The present study shows that its sclerotia can be reproduced from the hyphae, in addition to its traditionalmode of reprodu...Grifola umbellata (Pers.) Pilat is a fungus whose sclerotium has potent pharmaceutical values.The present study shows that its sclerotia can be reproduced from the hyphae, in addition to its traditionalmode of reproduction from sclerotia. On a culture medium, the hyphae first become swollen and enlargedfollowed by differentiation to form a highly organized structure, sclerotia. We demonstrate that at amicroscopic level, sclerotia derived from hyphae and sclerotia obtained in the field are similar, both havingcortex and medullary substances.展开更多
A new benzophenone, securiphenone A was isolated from the roots of Securidaca inappendiculata. Its structure was. determined as 2, 3-methylenedioxy-4-methoxybenzophenone by spectroscopie methods.
A new compound, named heteraltaic acid was isolated from the flowers of Heteropappus altaicus (willd) Novopokr.. The compound was elucidated as (5R, 6S, 8aS) –5- [2-(3-furyl ) ethyl-5, 6, 8a-trimethyl-4a, 5, 6, 7, 8,...A new compound, named heteraltaic acid was isolated from the flowers of Heteropappus altaicus (willd) Novopokr.. The compound was elucidated as (5R, 6S, 8aS) –5- [2-(3-furyl ) ethyl-5, 6, 8a-trimethyl-4a, 5, 6, 7, 8, 8a -hexahydro-1-naphthalenecarboxylic acid] by the combination of 1D and 2D NMR techniques (HSQC, HMBC) and X-ray analysis.展开更多
A new dihydroflavone glycoside was isolated from the underground parts of Glycyrrhiza uralensis. Its structure was elucidated as 7-hydroxyl-4'-O-β-D-(6'-O-α-hydroxylpropionyl) glucopyranosyl dihydroflavone b...A new dihydroflavone glycoside was isolated from the underground parts of Glycyrrhiza uralensis. Its structure was elucidated as 7-hydroxyl-4'-O-β-D-(6'-O-α-hydroxylpropionyl) glucopyranosyl dihydroflavone by spectral methods.展开更多
From the ethanolic extract of the whole plant of Hemistepta lyrata Bunge., a new lignan, named hemislin B glucoside, has been isolated and identified as a-(trans-3-methoxy-4-O-b-D-glucopyranosylbenzylidene)-b-(3,4-dim...From the ethanolic extract of the whole plant of Hemistepta lyrata Bunge., a new lignan, named hemislin B glucoside, has been isolated and identified as a-(trans-3-methoxy-4-O-b-D-glucopyranosylbenzylidene)-b-(3,4-dimethoxybenzyl)-g-butyrolactone. Its structure has been established on the basis of UV, IR, FABMS, 1H-NMR, 13C-NMR, HMQC and HMBC spectral analysis.展开更多
Starting from shikimic acid, the total synthesis of zeylenone was studied. The productwas proved to be the (+)antipode of zeylenone through analysis and comparison of their respectivespectra (including NMR, MS, IR and...Starting from shikimic acid, the total synthesis of zeylenone was studied. The productwas proved to be the (+)antipode of zeylenone through analysis and comparison of their respectivespectra (including NMR, MS, IR and CD) and optical data. The absolute configuration of thenatural product was thus determined to be (1S,2S,3R).展开更多
Marginatol (1), a new isopimarene diterpenoid, and five known compounds 8(14),15-sandera-copimaradiene-1α，9α-diol (2), 8(14), 15-sanderacopimaradene-1α 6β,9α-triol (3), germacrone (4), trans-ethyl p-methoxycinna...Marginatol (1), a new isopimarene diterpenoid, and five known compounds 8(14),15-sandera-copimaradiene-1α，9α-diol (2), 8(14), 15-sanderacopimaradene-1α 6β,9α-triol (3), germacrone (4), trans-ethyl p-methoxycinnamate (5) and n-pentadecane (6) were isolated from the hexane extract of Kaempferia marginata Carey.The structure of marginatol (1) was established as 8(14),15-isopimaradiene-6α-ol on the basis of spectral data (IR,MS，^1h-^-1H 13^C-^1H NOESY and HMBC). Compounds 2, 3 and 4 were isolated for the first time from thisplant.展开更多
基金Supported by the Fund of One Hundred Scientist Plan of Chinese Academy of Sciences and the Knowledge Innovation Program Foundation of Chinese Academy of Sciences,No.KSCX2-SW-207
文摘AIM: To investigate the effects of herbal compound 861 (Cpd 861) on cell proliferation in human hepatic stellate cells (LX-2) and human hepatocellular liver carcinoma cells (HepG2), and expression of α-smooth muscle actin (α-SMA) in LX-2 cells.METHODS: LX-2 and HepG2 cells were incubated with various concentrations of Cpd 861 (0.1-0.003 mg/mL)for 1, 2, 3, 5 and 7 d. Cell proliferation was analyzed by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Effects of Cpd861on the expression of cc-SMA mRNA in LX-2 cells weremeasured by real-time quantitative PCR method using SYBRGreen I technology.RESULTS: Cpd 861, at 0.1 mg/mL, significantly inhibitedLX-2 cell proliferation (15% decrease relative to control,P<0.05) after 3 d of incubation. The inhibitory effects seemedto increase with the treatment time (25% decrease after5 d of incubation and 35% decrease after 7 d of incubation,P<0.01). However, Cpd 861 did not affect HepG2 cellproliferation at the same concentration used for I_X-2 cells.The expression levels of ^-SMA mRNA decreased significantlywhen LX-2 cells were exposed to Cpd 861 for 48 h (59%decrease relative to control, P<0.05) or 72 h (60% decreaserelative to control, P<0.01).CONCLUSION: Cpd 861 can significantly inhibit LX-2 cellproliferation in a dose-dependant manner, and reduce theexpression levels of o^-SMA mRNA in LX-2 cells. Since hepaticcell proliferation and high level of ^-SMA are associatedwith liver fibrosis, the results suggest that Cpd 861 may beuseful in the treatment of this disease.
基金The authors would like to acknowledge Agilent Technologies for providing the capillary electrophoresis system and Bioanalytical System for present of sulfated b-CD. The study is supported by NSFC Grant No. 29875001.
文摘A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, concentration of running buffers, addition of sulfated b-CD, applied voltage and column temperature. Baseline separation was achieved for the nine xanthones in less than 15 minutes using a background electrolyte consisting of 200 mmol/L borate (pH 9.5) and 10 mmol/L sulfated b-CD.
文摘Grifola umbellata (Pers.) Pilat is a fungus whose sclerotium has potent pharmaceutical values.The present study shows that its sclerotia can be reproduced from the hyphae, in addition to its traditionalmode of reproduction from sclerotia. On a culture medium, the hyphae first become swollen and enlargedfollowed by differentiation to form a highly organized structure, sclerotia. We demonstrate that at amicroscopic level, sclerotia derived from hyphae and sclerotia obtained in the field are similar, both havingcortex and medullary substances.
文摘A new benzophenone, securiphenone A was isolated from the roots of Securidaca inappendiculata. Its structure was. determined as 2, 3-methylenedioxy-4-methoxybenzophenone by spectroscopie methods.
文摘A new compound, named heteraltaic acid was isolated from the flowers of Heteropappus altaicus (willd) Novopokr.. The compound was elucidated as (5R, 6S, 8aS) –5- [2-(3-furyl ) ethyl-5, 6, 8a-trimethyl-4a, 5, 6, 7, 8, 8a -hexahydro-1-naphthalenecarboxylic acid] by the combination of 1D and 2D NMR techniques (HSQC, HMBC) and X-ray analysis.
文摘A new dihydroflavone glycoside was isolated from the underground parts of Glycyrrhiza uralensis. Its structure was elucidated as 7-hydroxyl-4'-O-β-D-(6'-O-α-hydroxylpropionyl) glucopyranosyl dihydroflavone by spectral methods.
文摘From the ethanolic extract of the whole plant of Hemistepta lyrata Bunge., a new lignan, named hemislin B glucoside, has been isolated and identified as a-(trans-3-methoxy-4-O-b-D-glucopyranosylbenzylidene)-b-(3,4-dimethoxybenzyl)-g-butyrolactone. Its structure has been established on the basis of UV, IR, FABMS, 1H-NMR, 13C-NMR, HMQC and HMBC spectral analysis.
文摘Starting from shikimic acid, the total synthesis of zeylenone was studied. The productwas proved to be the (+)antipode of zeylenone through analysis and comparison of their respectivespectra (including NMR, MS, IR and CD) and optical data. The absolute configuration of thenatural product was thus determined to be (1S,2S,3R).
文摘Marginatol (1), a new isopimarene diterpenoid, and five known compounds 8(14),15-sandera-copimaradiene-1α，9α-diol (2), 8(14), 15-sanderacopimaradene-1α 6β,9α-triol (3), germacrone (4), trans-ethyl p-methoxycinnamate (5) and n-pentadecane (6) were isolated from the hexane extract of Kaempferia marginata Carey.The structure of marginatol (1) was established as 8(14),15-isopimaradiene-6α-ol on the basis of spectral data (IR,MS，^1h-^-1H 13^C-^1H NOESY and HMBC). Compounds 2, 3 and 4 were isolated for the first time from thisplant.
