AIM: To investigate the protective effects of early enteral feeding (EEF) on postburn impairments of renal function and their possible mechanisms.METHODS: Wistar rats with 30 % of total body surface area (TBSA) full-t...AIM: To investigate the protective effects of early enteral feeding (EEF) on postburn impairments of renal function and their possible mechanisms.METHODS: Wistar rats with 30 % of total body surface area (TBSA) full-thickness burn were adopted as the experimental model. The effects of EEF on the postburn changes of gastric intramucosal pH (pHi), endotoxin levels in portal vein, water contents of renal tissue, and blood concentrations of tumor necrosis factor (TNF-α), urea nitrogen (BUN), creatinine (Ct), as well as the changes of clearance of creatinine (CCr) were dynamically observed within 48 h postburn.RESULTS: EEF could significantly improve gastric mucosal acidosis, reduce portal vein endotoxin levels and water contents of renal tissue, as well as blood concentrations of TNF＜α after severe burns (P＜0.01). The postburn elevations of BUN and BCr were not found to be recovered by EEF.However, the CCr in EEF group was greatly increased by 4.67-fold compared with that of the non-feeding burned control (16.43±2.90 vs. 3.52±0.79, P＜0.01).CONCLUSION: EEF has beneficial effects on the improvement of renal function in severely burned rats, which may be related to its increase of splanchnic blood flow,decrease of the translocation of gut-origin endotoxin and the release of inflammatory mediators.展开更多
AIM: To assess the expression of α-catenin in gastric carcinoma and to determine the role of α-catenin expression in gastric carcinogenesis.METHODS: α-catenin expression was assessed by semi quantitative reverse tr...AIM: To assess the expression of α-catenin in gastric carcinoma and to determine the role of α-catenin expression in gastric carcinogenesis.METHODS: α-catenin expression was assessed by semi quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical staining in 49 gastric carcinomas,26 adjacent non-cancerous mucosae, and gastric biopsy specimens from 11 healthy controls.RESULTS: mRNA levels of α-catenin were reduced or absent in 34 of 49 (69%) gastric carcinoma tissues and in 5 of 26 (19%) tumor-free gastric mucosae of carcinomapatients, respectively. Of the carcinoma samples with altered α-catenin mRNA levels, α-catenin expression was negative in 20 and decreased in 14 cases. Up to 69% of tumors were stained abnormally for α-catenin. Of the 34 cases whose mRNA expression of α-catenin was reduced, 32 (94%) showed abnormal immunostaining patterns, while only 2 showed a normal α-catenin expression. The frequency of reduced expression of α-catenin mRNA was 14% in well-differentiated carcinomas, higher than that in poorlydifferentiated carcinomas (86%). A significant correlation was not shown between α-catenin expression and bothdepth of invasion and lymph node metastasis. Moreover, there was no statistical difference between loss or down-regulation of α-catenin mRNA and Helicobacter pylori ( H pylori) infection.CONCLUSION: Downregulation of α-catenin expressionis common in gastric carcinoma, and α-catenin expression may be used as a differentiation marker. Downregulation of α-catenin expression may be an early event in tumorigenesis. Reduced α-catenin expression is not correlated with H pylori infection.展开更多
基金National Natural Science Foundation of China,No.30290700
文摘AIM: To investigate the protective effects of early enteral feeding (EEF) on postburn impairments of renal function and their possible mechanisms.METHODS: Wistar rats with 30 % of total body surface area (TBSA) full-thickness burn were adopted as the experimental model. The effects of EEF on the postburn changes of gastric intramucosal pH (pHi), endotoxin levels in portal vein, water contents of renal tissue, and blood concentrations of tumor necrosis factor (TNF-α), urea nitrogen (BUN), creatinine (Ct), as well as the changes of clearance of creatinine (CCr) were dynamically observed within 48 h postburn.RESULTS: EEF could significantly improve gastric mucosal acidosis, reduce portal vein endotoxin levels and water contents of renal tissue, as well as blood concentrations of TNF＜α after severe burns (P＜0.01). The postburn elevations of BUN and BCr were not found to be recovered by EEF.However, the CCr in EEF group was greatly increased by 4.67-fold compared with that of the non-feeding burned control (16.43±2.90 vs. 3.52±0.79, P＜0.01).CONCLUSION: EEF has beneficial effects on the improvement of renal function in severely burned rats, which may be related to its increase of splanchnic blood flow,decrease of the translocation of gut-origin endotoxin and the release of inflammatory mediators.
基金Supported by the Scientific Foundation of Gansu Province, No. QS022-C33-029
文摘AIM: To assess the expression of α-catenin in gastric carcinoma and to determine the role of α-catenin expression in gastric carcinogenesis.METHODS: α-catenin expression was assessed by semi quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical staining in 49 gastric carcinomas,26 adjacent non-cancerous mucosae, and gastric biopsy specimens from 11 healthy controls.RESULTS: mRNA levels of α-catenin were reduced or absent in 34 of 49 (69%) gastric carcinoma tissues and in 5 of 26 (19%) tumor-free gastric mucosae of carcinomapatients, respectively. Of the carcinoma samples with altered α-catenin mRNA levels, α-catenin expression was negative in 20 and decreased in 14 cases. Up to 69% of tumors were stained abnormally for α-catenin. Of the 34 cases whose mRNA expression of α-catenin was reduced, 32 (94%) showed abnormal immunostaining patterns, while only 2 showed a normal α-catenin expression. The frequency of reduced expression of α-catenin mRNA was 14% in well-differentiated carcinomas, higher than that in poorlydifferentiated carcinomas (86%). A significant correlation was not shown between α-catenin expression and bothdepth of invasion and lymph node metastasis. Moreover, there was no statistical difference between loss or down-regulation of α-catenin mRNA and Helicobacter pylori ( H pylori) infection.CONCLUSION: Downregulation of α-catenin expressionis common in gastric carcinoma, and α-catenin expression may be used as a differentiation marker. Downregulation of α-catenin expression may be an early event in tumorigenesis. Reduced α-catenin expression is not correlated with H pylori infection.
