Eight two-rowed barley cultivars were grown at seven locations in the southern winter-barley zone of China. Mean grainβ-glucanase activity ranged from 39.89 U kg-1 for Suyin2 to 49.75 U kg-1 for Xi-umai3 in 8 cultiva...Eight two-rowed barley cultivars were grown at seven locations in the southern winter-barley zone of China. Mean grainβ-glucanase activity ranged from 39.89 U kg-1 for Suyin2 to 49.75 U kg-1 for Xi-umai3 in 8 cultivars grown at 7 locations, and from 38. 74 U kg-1 in Zhengzhou to 57. 96 U kg-1 in Putian among 7 locations on an average of all cultivars. Correspondingly, mean malt β-glucanase activity of 8 cultivars ranged from 313.33 U kg-1 for ZAU3 to 489. 89 U kg-1 for Daner Barley, and of 7 locations from 330.40 U kg-1 in Yancheng to 418. 24 U kg-1 in Putian. There were significant differences among cultivars and locations in maltβ-glucanase activities. The locations showed much larger variation in maltβ-glucanase activities than cultivars. The reduction of total β-glucan content after malting varied in both cultivars and locations, with a mean of 78.31%. The analysis of correlations showed that maltβ-glucan content was significantly positively and negatively correlated with grain β-glucan content and malt β-glucanase activity, respectively, and malt β-glucanase activity was significantly positively correlated with grain β-glucanase activity.展开更多
Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH...Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20 ℃) . Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R=40.0,42.4, 48.0, 62.8, 107.0).. The results indicated that the most important factor was the nature of the strain (R= 107.0),. while the change in temperature and time of cultivation produced the lowest effect (R= 40.0).. Each factor variance was significant and A 3B 4C 2D 1E 3 was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most.展开更多
Trichoderma aureoviride mutant T1010 was cultured in media containing different nutrients. Statistics showed significant differences and the results described below: The C/N tested were 3∶1, 6∶1, 12∶1, 24∶1,48∶1....Trichoderma aureoviride mutant T1010 was cultured in media containing different nutrients. Statistics showed significant differences and the results described below: The C/N tested were 3∶1, 6∶1, 12∶1, 24∶1,48∶1. The best C/N was 24∶1. The amount of spores was 4.0×109 cfu/plate. The best carbon source among glucose, sucrose, maltose, lactose, cellulose and starch was the last one. With a starch-containing medium, T1010 produced 5.3×109 cfu/plate. The asparagine was the best nitrogen source. T1010 absorbed it easily and produced 9.6×109 cfu/plate. K, Mg, P, S, Fe were important to support T1010 growth and sporulation. In the absence of K, P, and Fe, Trichoderma grew poorly, while Mg didn’t seem to help Trichoderma colony development. A little amount of vitamins allwed T1010 to grow better. This data allowed us to improve the cultivation of Trichoderma at industrial level.展开更多
In rice field, especially in the fertilized field the combined nitrogen content is rather high. It is well known that the nitrogen compounds, particularly NH4^+ inhibits the nitrogenase activity- of diazotrophs. So, t...In rice field, especially in the fertilized field the combined nitrogen content is rather high. It is well known that the nitrogen compounds, particularly NH4^+ inhibits the nitrogenase activity- of diazotrophs. So, the enhancement of nitrogen fixation ability of diazotrophs in the presence of ammonia by means of genetic manipulation will be of importance for agricultural use.展开更多
文摘Eight two-rowed barley cultivars were grown at seven locations in the southern winter-barley zone of China. Mean grainβ-glucanase activity ranged from 39.89 U kg-1 for Suyin2 to 49.75 U kg-1 for Xi-umai3 in 8 cultivars grown at 7 locations, and from 38. 74 U kg-1 in Zhengzhou to 57. 96 U kg-1 in Putian among 7 locations on an average of all cultivars. Correspondingly, mean malt β-glucanase activity of 8 cultivars ranged from 313.33 U kg-1 for ZAU3 to 489. 89 U kg-1 for Daner Barley, and of 7 locations from 330.40 U kg-1 in Yancheng to 418. 24 U kg-1 in Putian. There were significant differences among cultivars and locations in maltβ-glucanase activities. The locations showed much larger variation in maltβ-glucanase activities than cultivars. The reduction of total β-glucan content after malting varied in both cultivars and locations, with a mean of 78.31%. The analysis of correlations showed that maltβ-glucan content was significantly positively and negatively correlated with grain β-glucan content and malt β-glucanase activity, respectively, and malt β-glucanase activity was significantly positively correlated with grain β-glucanase activity.
文摘Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone + chlorothalonil, or maneb or phosethyl-Al) . The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20 ℃) . Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R=40.0,42.4, 48.0, 62.8, 107.0).. The results indicated that the most important factor was the nature of the strain (R= 107.0),. while the change in temperature and time of cultivation produced the lowest effect (R= 40.0).. Each factor variance was significant and A 3B 4C 2D 1E 3 was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most.
文摘Trichoderma aureoviride mutant T1010 was cultured in media containing different nutrients. Statistics showed significant differences and the results described below: The C/N tested were 3∶1, 6∶1, 12∶1, 24∶1,48∶1. The best C/N was 24∶1. The amount of spores was 4.0×109 cfu/plate. The best carbon source among glucose, sucrose, maltose, lactose, cellulose and starch was the last one. With a starch-containing medium, T1010 produced 5.3×109 cfu/plate. The asparagine was the best nitrogen source. T1010 absorbed it easily and produced 9.6×109 cfu/plate. K, Mg, P, S, Fe were important to support T1010 growth and sporulation. In the absence of K, P, and Fe, Trichoderma grew poorly, while Mg didn’t seem to help Trichoderma colony development. A little amount of vitamins allwed T1010 to grow better. This data allowed us to improve the cultivation of Trichoderma at industrial level.
文摘In rice field, especially in the fertilized field the combined nitrogen content is rather high. It is well known that the nitrogen compounds, particularly NH4^+ inhibits the nitrogenase activity- of diazotrophs. So, the enhancement of nitrogen fixation ability of diazotrophs in the presence of ammonia by means of genetic manipulation will be of importance for agricultural use.
