Chinese cherry （Prunus pseudocerasus L.） is an allotetraploid species and exhibits natural self-compatibility.However,the full-length cDNA sequences,functional analysis and the transcripts of S-RNase alleles in Chin...Chinese cherry （Prunus pseudocerasus L.） is an allotetraploid species and exhibits natural self-compatibility.However,the full-length cDNA sequences,functional analysis and the transcripts of S-RNase alleles in Chinese cherry cultivars are not known.In the two cultivars Taixiaohongying and Laiyang Short Cherry with S1S2S3S4 genotypes,two S-RNases were transcribed in Northern blotting,and the two full-length cDNAs of S-RNase were cloned and analyzed.As the result,the transcribed S-RNases were S1-RNase and S2-RNase.The two complete cDNA sequences of S1-RNase and S2-RNase were registered as EU073938 and EU073939,respectively,and had characteristic structure of rosaceous S-RNases based on their sequences indicating that they had normal function for S-RNase in the style.The S3-RNase and S4-RNase were not transcribed in the style and were nonfunctional for S-RNase,so S3m and S4m could be used to represent the nonfunctional S3-RNase and S4-RNase.The phylogenetic analysis implied that the S-RNases of Prunus,including Chinese cherry,had lower intra-specific similarity and diverged earlier than the divergence of species in Prunus.展开更多
The made tea quality and chemical characteristics of 4 albinos cultivars were investigated. It shows that constitutes of micro-elements, contents of polyphenols and total amino acids in 4 samples are much different du...The made tea quality and chemical characteristics of 4 albinos cultivars were investigated. It shows that constitutes of micro-elements, contents of polyphenols and total amino acids in 4 samples are much different due to the biological diversity. The DPPH free radicals scavenging ability of the 4 albinos tea is also different. The free radicals scavenging rate has a significant positive correlation to the content of total polyphenols, total catechins, as well as C, CG, GCG, EGCG, but there is no positive relationship to the content of Zn, Se.展开更多
基金supported by the National 863 Program of China (2006AA100108)the Doctoral Fund of Shandong Province,China (2006BS06021)Fine Cultivar Program of Shandong Province,China
文摘Chinese cherry （Prunus pseudocerasus L.） is an allotetraploid species and exhibits natural self-compatibility.However,the full-length cDNA sequences,functional analysis and the transcripts of S-RNase alleles in Chinese cherry cultivars are not known.In the two cultivars Taixiaohongying and Laiyang Short Cherry with S1S2S3S4 genotypes,two S-RNases were transcribed in Northern blotting,and the two full-length cDNAs of S-RNase were cloned and analyzed.As the result,the transcribed S-RNases were S1-RNase and S2-RNase.The two complete cDNA sequences of S1-RNase and S2-RNase were registered as EU073938 and EU073939,respectively,and had characteristic structure of rosaceous S-RNases based on their sequences indicating that they had normal function for S-RNase in the style.The S3-RNase and S4-RNase were not transcribed in the style and were nonfunctional for S-RNase,so S3m and S4m could be used to represent the nonfunctional S3-RNase and S4-RNase.The phylogenetic analysis implied that the S-RNases of Prunus,including Chinese cherry,had lower intra-specific similarity and diverged earlier than the divergence of species in Prunus.
文摘The made tea quality and chemical characteristics of 4 albinos cultivars were investigated. It shows that constitutes of micro-elements, contents of polyphenols and total amino acids in 4 samples are much different due to the biological diversity. The DPPH free radicals scavenging ability of the 4 albinos tea is also different. The free radicals scavenging rate has a significant positive correlation to the content of total polyphenols, total catechins, as well as C, CG, GCG, EGCG, but there is no positive relationship to the content of Zn, Se.
