To maintain soil quality under long-term saline water irrigation,the influence of manure on soil physical properties was examined.Long-term saline irrigation has been conducted from 2015 to 2024 at the Nanpi Eco-Agric...To maintain soil quality under long-term saline water irrigation,the influence of manure on soil physical properties was examined.Long-term saline irrigation has been conducted from 2015 to 2024 at the Nanpi Eco-Agricultural Experimental Station of Chinese Academy Sciences in the Low Plain of the North China Plain,comprising four irrigation treatments:irrigation once at the jointing stage for winter wheat with irrigation water containing salt at fresh water,3,4 and 5 g·L^(–1),and maize irrigation at sowing using fresh water.Manure application was conducted under all irrigation treatments,with treatments without manure application used as controls.The results showed that under long-term irrigation with saline water,the application of manure increased the soil organic matter content,exchangeable potassium,available phosphorus,and total nitrogen content in the 0–20 cm soil layer by 46.8%,117.0%,75.7%,and 45.5%,respectively,compared to treatments without manure application.The application of manure reduced soil bulk density.It also increased the proportion of water-stable aggregates and the abundance of bacteria,fungi,and actinomycetes in the tillage soil layer compared to the controls.Because of the salt contained in the manure,the application of manure had dual effects on soil salt content.During the winter wheat season,manure application increased soil salt content.The salt content was significantly reduced during the summer maize season,owing to the strong salt-leaching effects under manure application,resulting in a smaller difference in salt content between the manure and non-manure treatments.During the summer rainfall season,improvements in soil structure under manure application increased the soil desalination rate for the 1 m top soil layer.The desalination rate for 0–40 cm and 40–100 cm was averagely by 39.1%and 18.9%higher,respectively,under manure application as compared with that under the nomanure treatments.The yield of winter wheat under manure application was 0.12%lower than that of the control,owing to the higher salt content during the winter wheat season.In contrast,the yield of summer maize improved by 3.9%under manure application,owing to the increased soil nutrient content and effective salt leaching.The results of this study indicated that manure application helped maintain the soil physical structure,which is important for the long-term use of saline water.In practice,using manure with a low salt content is suggested to reduce the adverse effects of saline water irrigation on soil properties and achieve sustainable saline water use.展开更多
Safflower is an important oilseed crop that has been used in traditional Chinese medicine for thousands of years because of the clinically valuable flavonoid glycosides in its flower petals.However,the biosynthesis an...Safflower is an important oilseed crop that has been used in traditional Chinese medicine for thousands of years because of the clinically valuable flavonoid glycosides in its flower petals.However,the biosynthesis and molecular regulation of these compounds are still elusive due to the lack of a high-quality reference genome and scarce identification of key biosynthetic pathway genes in a medicinal safflower variety.Here we leveraged an integrative multi-omics strategy by combining genomic,comparative genomics,and tissue-specific transcriptome profiling with biochemical analysis to identify uridine diphosphate glycosyltransferases(UGTs)for flavonoid glycoside biosynthesis in safflower.We assembled and annotated a high-quality reference genome of a medicinal safflower variety,‘Yunhong3’.A comprehensive comparative genomic analysis indicated that an evolutionary whole-genome triplication event occurring in safflower contributed to gene amplification of the flavonoid biosynthetic pathway.By combining comparative transcriptome profiling with enzymatic reactions,we identified 11 novel UGTs that could catalyze the conversion of naringenin chalcone and phloretin to the corresponding O-glycosides.Moreover,we outlined the molecular pathway of hydroxysafflor yellow A(HSYA)biosynthesis featured by 17 newly identified UGTs with promising catalytic activity,laying the foundation for the synthetic production of HSYA.Our study reports systemic genome and gene expression information for flavonoid glycoside biosynthesis in medicinal safflower and provides insights into mechanisms regulating HSYA biosynthesis,which would facilitate the genetic improvement and synthetic bioengineering design for producing clinically valuable flavonoid glycosides in safflower.展开更多
Epigenetics refers to the study of heritable changes in gene function that do not involve changes in the DNA sequence. Such effects on cellular and physiological phenotypic traits may result from external or environme...Epigenetics refers to the study of heritable changes in gene function that do not involve changes in the DNA sequence. Such effects on cellular and physiological phenotypic traits may result from external or environmental factors or be part of normal developmental program. In eukaryotes, DNA wraps on a histone octamer(two copies of H2A, H2B, H3 and H4) to form nucleosome, the fundamental unit of chromatin. The structure of chromatin is subjected to a dynamic regulation through multiple epigenetic mechanisms, including DNA methylation, histone posttranslational modifications(PTMs), chromatin remodeling and noncoding RNAs. As conserved regulatory mechanisms in gene expression, epigenetic mechanisms participate in almost all the important biological processes ranging from basal development to environmental response. Importantly, all of the major epigenetic mechanisms in mammalians also occur in plants. Plant studies have provided numerous important contributions to the epigenetic research. For example, gene imprinting, a mechanism of parental allele-specific gene expression, was firstly observed in maize; evidence of paramutation, an epigenetic phenomenon that one allele acts in a single locus to induce a heritable change in the other allele, was firstly reported in maize and tomato.Moreover, some unique epigenetic mechanisms have been evolved in plants. For example, the 24-nt siRNA-involved RNA-directed DNA methylation(RdDM) pathway is plant-specific because of the involvements of two plant-specific DNA-dependent RNA polymerases, Pol IV and Pol V. A thorough study of epigenetic mechanisms is of great significance to improve crop agronomic traits and environmental adaptability. In this review, we make a brief summary of important progress achieved in plant epigenetics field in China over the past several decades and give a brief outlook on future research prospects.We focus our review on DNA methylation and histone PTMs, the two most important aspects of epigenetic mechanisms.展开更多
Nonalcoholic fatty liver disease （NAFLD） is the most common liver disease in the world today. Its incidence in adults and children is rising rapidly due to the ongoing epidemics of obesity and type 2 diabetes. Hence...Nonalcoholic fatty liver disease （NAFLD） is the most common liver disease in the world today. Its incidence in adults and children is rising rapidly due to the ongoing epidemics of obesity and type 2 diabetes. Hence, it has become a global public health issue. Envi- ronmental factors have been found to play a major role in the etiology of NAFLD, especially for genetically susceptible populations. Among these, one of the most important factors is junk food, especially the typical ＂Western-style＂ diet rich in simple carbohydrates, saturated fat, and highly processed food materials. Genetic predisposition to NAFLD does occur; however, a precise definition of genetic factors responsible for NAFLD is still lacking. Specific variants of different genes have been shown to present a risk for NAFLD. Genetic studies might be helpful in the management of the disease by developing novel treatment strategies based on individual＇s genotype.展开更多
Plant centromeres are generally composed of tandem arrays of simple repeats that form a complex chromosome locus where the kinetochore forms and microtubules attach during mitosis and meiosis. Each chromosome has one ...Plant centromeres are generally composed of tandem arrays of simple repeats that form a complex chromosome locus where the kinetochore forms and microtubules attach during mitosis and meiosis. Each chromosome has one centromere region, which is essential for accurate division of the genetic material. Recently, chromosomes containing two centromere regions （called dicentric chromosomes） have been found in maize and wheat. Interestingly, some dicentric chromosomes are stable because only one centromere is active and the other one is inactivated. Because such arrays maintain their typical structure for both active and inactive centromeres, the specification of centromere activity has an epigenetic component independent of the DNA sequence. Under some circumstances, the inactive centromeres may recover centromere function, which is called centromere reactivation. Recent studies have highlighted the important changes, such as DNA methylation and histone modification, that occur during centromere inactivation and reactivation.展开更多
The structure and shape of a cotton shrub aredetermined in result of combining theirelements:length and amount internodes of amain stem and fruit brunches,amount and shapeof leaf blades.Some lines of genetic collectio...The structure and shape of a cotton shrub aredetermined in result of combining theirelements:length and amount internodes of amain stem and fruit brunches,amount and shapeof leaf blades.Some lines of genetic collectionhave been crossed between each other for展开更多
Journal of Genetics and Genomics(JGG),launched in 1974,celebrates its 50th birthday in 2023.With continuous support from our authors,reviewers,readers,and the editorial board,JGG has made remarkable progress in the pa...Journal of Genetics and Genomics(JGG),launched in 1974,celebrates its 50th birthday in 2023.With continuous support from our authors,reviewers,readers,and the editorial board,JGG has made remarkable progress in the past year by publishing 122 papers covering major disciplines in life sciences and medical genetics with exciting discoveries.It is worthwhile to note that we have recruited 66 young talents to join JGG as junior editors,resulting in significant improvement in manuscript evaluation and journal promotion.Here,we summarize major progresses reported in JGG in 2023 fromaneditorial view.展开更多
This erratum clarifies information in the article“Nucleolar histone deacetylases HDT1,HDT2,and HDT3 regulate plant reproductive development”by Luo et al.(2021).In the section on“Phenotype of hdt mutants”,the T-DNA...This erratum clarifies information in the article“Nucleolar histone deacetylases HDT1,HDT2,and HDT3 regulate plant reproductive development”by Luo et al.(2021).In the section on“Phenotype of hdt mutants”,the T-DNA insertional knockdown mutant for HDT2(hdt2,salk_1247_A02)should be“Sail_1247_A02”in the main text and Fig.3A.展开更多
This erratum clarifies information in the Letter to the Editor“Actin polymerization induces mitochondrial distribution during collective cell migration”by Qu et al.(2023).In the section for the list of author names,...This erratum clarifies information in the Letter to the Editor“Actin polymerization induces mitochondrial distribution during collective cell migration”by Qu et al.(2023).In the section for the list of author names,“Chen Qu,Yating Kan,Hui Zuo,Mengqi Wu,Zhixiang Dong,Xinyi Wang,Qing Zhang,Heng Wang,Dou Wang,Jiong Chen”should be“Chen Qu,Yating Kan,Xinyi Wang,Hui Zuo,Mengqi Wu,Zhixiang Dong,Qing Zhang,Heng Wang,Dou Wang,Jiong Chen”.展开更多
<p style="text-align:justify;"> <span style="font-family:""><span style="font-family:Verdana;">In the article, the first time genes of anthocyanin pigmentation R<...<p style="text-align:justify;"> <span style="font-family:""><span style="font-family:Verdana;">In the article, the first time genes of anthocyanin pigmentation R</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;"> and </span></span><span style="font-family:""><span style="font-family:Verdana;">R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span><span style="font-family:Verdana;"> appeared in cotton </span><span style="font-family:Verdana;">plant</span><span style="font-family:Verdana;"> interacting complementarily. Recessive homozygosis r</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">r</span></span></span><sub><span style="font-family:Verdana;">р</span></sub><span style="font-family:""><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span><span style="font-family:Verdana;"> conditions the development of green </span><span style="font-family:Verdana;">colour</span><span style="font-family:Verdana;">. The gen </span></span><span style="font-family:Verdana;">R</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;"> conditions the development of anthocyanin in all organs of plants, </span><span style="font-family:Verdana;">R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span><span style="font-family:Verdana;"> only exists on the stalk </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> in the nerves of leaves, the flower is uncolored, the boll is green. The plants’ existence in </span><span style="font-family:Verdana;">genotype</span><span style="font-family:Verdana;"> of both dominant </span><span style="font-family:Verdana;">equilocal</span><span style="font-family:Verdana;"> genes (R</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">, R</span><sub><span style="font-family:Verdana;">st</span></sub><span style="font-family:Verdana;">) provides </span></span></span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">high</span><span style="font-family:Verdana;"> rate of biosynthesis of anthocyanin</span><span style="font-family:""><span style="font-family:Verdana;">—</span><span style="font-family:Verdana;">dark red. </span></span><span style="font-family:Verdana;">Inheritance of the chlorophylls “a” + </span><span style="font-family:Verdana;">“</span><span style="font-family:""><span style="font-family:Verdana;">b” and “a” and “b” quantity in anthocyanin </span><span style="font-family:Verdana;">coloured</span><span style="font-family:Verdana;"> L-2 and L-3 lines of F</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;"> and F</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> generations. The </span><span style="font-family:Verdana;">quantity</span><span style="font-family:Verdana;"> of chlorophylls in anthocyanin </span><span style="font-family:Verdana;">coloured</span><span style="font-family:Verdana;"> L-3 line was defined 1.0</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">1.5 times higher and participation of the gen R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;"> responsible for anthocyanin </span><span style="font-family:Verdana;">colour</span><span style="font-family:Verdana;"> in chlorophylls biosynthesis was also defined. Apparently, in </span><span style="font-family:Verdana;">the line</span><span style="font-family:Verdana;"> L-3 in R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;"> is localized</span></span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> there are gens </span><span style="font-family:Verdana;">which are</span><span style="font-family:Verdana;"> responsible for the quantity of chlorophyll or R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;"> has </span><span style="font-family:Verdana;">pleiotropic</span><span style="font-family:Verdana;"> effect</span></span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> on the quantity of chlorophyll. It is proved with </span><span style="font-family:Verdana;">low</span><span style="font-family:Verdana;"> quantity of chlorophyll in green </span><span style="font-family:Verdana;">coloured</span><span style="font-family:Verdana;"> plants by recessive homozygote condition </span><span><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup></span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">green </span><span style="font-family:Verdana;">colour</span><span style="font-family:Verdana;">.</span></span> </p>展开更多
Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage...Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.展开更多
Branching is a critical aspect of plant architecture that significantly impacts the yield and adaptability of staple cereal crops like rice and wheat.Cereal crops develop tillers during the vegetative stage and panicl...Branching is a critical aspect of plant architecture that significantly impacts the yield and adaptability of staple cereal crops like rice and wheat.Cereal crops develop tillers during the vegetative stage and panicle or spike branches during the reproductive stage,respectively,both of which are significantly impacted by hormones and genetic factors.Tillering and panicle branching are closely interconnected and exhibit high environmental plasticity.Here,we summarize the recent progress in genetic,hormonal,and environmental factors regulation in the branching of rice and wheat.This review not only provides a comprehensive overview of the current knowledge on branching mechanisms in rice and wheat,but also explores the prospects for future research aimed at optimizing crop architecture for enhanced productivity.展开更多
As a result of long standing investigation onurgent problems of amphidiploid cotton speciesGossypium hirsutum genetics,for the first timegenetic determination of the most importanttraits has been established,and the u...As a result of long standing investigation onurgent problems of amphidiploid cotton speciesGossypium hirsutum genetics,for the first timegenetic determination of the most importanttraits has been established,and the uniquegenetic collection of homozygous isogenic lineshas been developed.A scientifically well-founded theory about combined types ofpolygene interaction in genetic determination oftracts in cotton,has been developed.展开更多
Originally extracted from willow bark,salicylic acid(SA)provided the structural basis for the synthesis of acetylsalicylic acid(aspirin)in 1897,a milestone that exemplifies the far-reaching biomedical relevance of pla...Originally extracted from willow bark,salicylic acid(SA)provided the structural basis for the synthesis of acetylsalicylic acid(aspirin)in 1897,a milestone that exemplifies the far-reaching biomedical relevance of plant-derived metabolites(Desborough and Keeling,2017).In plants,SA functions as a pleiotropic phytohormone that orchestrates immune reprogramming,serving as a central mediator of both local defense responses and systemic acquired resistance(SAR).展开更多
Reactive oxygen species(ROS)and nitric oxide(NO)are two critical classes of signaling molecules that regulate plant development and stress responses.The intracellular level of S-nitrosoglutathione(GSNO),a major bioact...Reactive oxygen species(ROS)and nitric oxide(NO)are two critical classes of signaling molecules that regulate plant development and stress responses.The intracellular level of S-nitrosoglutathione(GSNO),a major bioactive NO species,is regulated by the highly conserved GSNO reductase(GSNOR).However,the molecular mechanisms underlying ROS-mediated regulation of GSNOR remain largely unclear.Here,we show that H_(2)O_(2)negatively regulates the activity of GSNOR1 during ovule development in Arabidopsis.S-sulfenylation of GSNOR1 at Cys-284 inhibits its enzymatic activity.A GSNOR1C284S mutation causes a reduction of the total SNO level in pistils,thereby disrupting NO homeostasis and eventually leading to defective ovule development.These findings illustrate a unique mechanism by which ROS regulates ovule development through S-sulfenylation-mediated inhibition of the GSNOR activity,thereby establishing a molecular link between ROS and NO signaling pathways in reproductive development.展开更多
In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and t...In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.展开更多
Sulfate transporters(SULTRs)facilitate sulfate uptake and transport in plants.In plants,SULTRs can be classified into four distinct functional groups,among which SULTR3 members are the least characterized,and their fu...Sulfate transporters(SULTRs)facilitate sulfate uptake and transport in plants.In plants,SULTRs can be classified into four distinct functional groups,among which SULTR3 members are the least characterized,and their functions have not yet been confirmed,especially for SULTR3 in rice.In this study,we analyzed the expression patterns,subcellular localization,and inorganic phosphate(Pi)transporter activity of SULTR3 proteins in yeast.Except for OsSULTR3.4,which localized to the plasma membrane,other OsSULTR3 members were localized to the endoplasmic reticulum(ER)membrane in rice protoplast cells.展开更多
Vegetation response to the combined effects of human and nature has raised extensive controversy.The relative contributions of ecological restoration and multiple climate factors on vegetation variation remain unclear...Vegetation response to the combined effects of human and nature has raised extensive controversy.The relative contributions of ecological restoration and multiple climate factors on vegetation variation remain unclear.Combining spatial and temporal changes in fractional vegetation cover(FVC)and net primary productivity(NPP)from 2000 to 2021,we assessed the trend of these two indicators and quantified the impact of ecological restoration and climate factors on vegetation variation using partial correlation and residual analysis.The results indicated that:(1)From 2000 to 2021,vegetation restoration in the Taihang Mountain area was notably successful.FVC increased by 5.71%over 22 years with a change trend of 0.0018 per annum.The cumulative growth rate of NPP over the same period was 42.03%,corresponding to an average annual increase of 5.492 g(C)·m^(-2)·a^(-1).(2)During the 22-year period,the contribution rates of ecological engineering to FVC and NPP were 65.13%and 64.33%,respectively.Ecological restoration was the dominant factor affecting vegetation change,and its impact was increasing in over 90%of the regional projects.The primary climate controlling factor of NPP was precipitation,while FVC responded synergistically to precipitation(primary)and temperature(secondary).Overall,the impact of precipitation on vegetation was 1.5 times that of temperature.(3)Among various ecological restoration types,grassland restoration contributed the most to vegetation change,followed by forest land restoration.This study offers valuable scientific insights that can guide the sustainability of vegetation ecology in semi-arid mountainous areas.展开更多
Nitrate(NO_(3)^(-))accumulation and transport processes in the thick vadose zone affect the evolution of the groundwater NO_(3)^(-)content in intensive agricultural regions.Agricultural land-use change(ALUC),typically...Nitrate(NO_(3)^(-))accumulation and transport processes in the thick vadose zone affect the evolution of the groundwater NO_(3)^(-)content in intensive agricultural regions.Agricultural land-use change(ALUC),typically accompanied by substantial alterations in nitrogen fertilizer application and irrigation practices,is an important influencing factor.This study evaluated the changes in NO_(3)^(-)accumulation and transport in the deep vadose zone(DVZ,below the root zone),and the groundwater NO_(3)^(-)content associated with ALUC from grain to vegetable fields in the North China Plain(NCP).The ALUC from grain to vegetable resulted in nitrate–nitrogen(NO_(3)^(-)-N)accumulation in DVZ increased by 235.5 kg ha^(-1)m^(-1)(163.2%)in the piedmont plain and 224.9 kg ha^(-1)m^(-1)(102.7%)in the central plain,respectively.This change accelerated downward transport velocity in the DVZ(from 0.81±0.47 to 0.89±0.55 m yr^(-1)in the piedmont plain,and from 0.24±0.12 to 0.92±0.12 m yr^(-1)in the central plain)and increased NO_(3)^(-)leaching fluxes.High transport velocity and leaching fluxes resulted in chemical N-fertilizer entering the aquifer in several areas in the piedmont plain.The impact of the agricultural activity intensity changes,accompanied by the ALUC,on groundwater quantity and quality should be considered in similar regions.展开更多
Crop productivity may be affected by soil salinization from brackish water irrigation.Plants host species-specific soil microorganisms that can enhance plant adaptability to salt stress.However,it is unclear whether t...Crop productivity may be affected by soil salinization from brackish water irrigation.Plants host species-specific soil microorganisms that can enhance plant adaptability to salt stress.However,it is unclear whether these specific microorganisms recruited under brackish water irrigation are related to microbial resistance,which has been proven to promote crop production.A field experiment was conducted using five local wheat varieties,each of which was exposed to brackish or fresh water irrigation for four years.Brackish water irrigation tended to increase wheat yield by 6.38%–19.40%,although the increase in yield under brackish water relative to fresh water irrigation varied with wheat variety.The compositions of the bacterial and fungal communities in the bulk soil and rhizosphere were measured,and the taxa enriched following brackish water irrigation were isolated to investigate microbial resistance.We found that the resistance of each wheat variety was determined by specifically recruited microbial taxa with relevant functions.The yield incremental rates were positively regulated by microbial resistance from the bulk soil and rhizosphere.Moreover,the resistance from the bulk soil and rhizosphere had similar effects,and microbial consortia containing both beneficial and harmful taxa determined microbial resistance.These results indicated that brackish water irrigation-induced recruitment of specific microbial taxa from either the bulk soil or the rhizosphere or both positively contributed to the yield incremental rate and provided a list of key taxa linked to the magnitude of yield variation caused by brackish water irrigation.展开更多
基金supported by National Key R&D Program of China (2022YFD1900104)。
文摘To maintain soil quality under long-term saline water irrigation,the influence of manure on soil physical properties was examined.Long-term saline irrigation has been conducted from 2015 to 2024 at the Nanpi Eco-Agricultural Experimental Station of Chinese Academy Sciences in the Low Plain of the North China Plain,comprising four irrigation treatments:irrigation once at the jointing stage for winter wheat with irrigation water containing salt at fresh water,3,4 and 5 g·L^(–1),and maize irrigation at sowing using fresh water.Manure application was conducted under all irrigation treatments,with treatments without manure application used as controls.The results showed that under long-term irrigation with saline water,the application of manure increased the soil organic matter content,exchangeable potassium,available phosphorus,and total nitrogen content in the 0–20 cm soil layer by 46.8%,117.0%,75.7%,and 45.5%,respectively,compared to treatments without manure application.The application of manure reduced soil bulk density.It also increased the proportion of water-stable aggregates and the abundance of bacteria,fungi,and actinomycetes in the tillage soil layer compared to the controls.Because of the salt contained in the manure,the application of manure had dual effects on soil salt content.During the winter wheat season,manure application increased soil salt content.The salt content was significantly reduced during the summer maize season,owing to the strong salt-leaching effects under manure application,resulting in a smaller difference in salt content between the manure and non-manure treatments.During the summer rainfall season,improvements in soil structure under manure application increased the soil desalination rate for the 1 m top soil layer.The desalination rate for 0–40 cm and 40–100 cm was averagely by 39.1%and 18.9%higher,respectively,under manure application as compared with that under the nomanure treatments.The yield of winter wheat under manure application was 0.12%lower than that of the control,owing to the higher salt content during the winter wheat season.In contrast,the yield of summer maize improved by 3.9%under manure application,owing to the increased soil nutrient content and effective salt leaching.The results of this study indicated that manure application helped maintain the soil physical structure,which is important for the long-term use of saline water.In practice,using manure with a low salt content is suggested to reduce the adverse effects of saline water irrigation on soil properties and achieve sustainable saline water use.
基金supported by the ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302)the National Key R&D Program of China(Grant No.2018YFA0900603)+1 种基金the National Key R&D Program of China(Grant No.2020YFA0908000)the crosswise task based on DEYUANTANG pharmacy Co.,Ltd.Shanxi,China(Grant No.DYTKY180725).
文摘Safflower is an important oilseed crop that has been used in traditional Chinese medicine for thousands of years because of the clinically valuable flavonoid glycosides in its flower petals.However,the biosynthesis and molecular regulation of these compounds are still elusive due to the lack of a high-quality reference genome and scarce identification of key biosynthetic pathway genes in a medicinal safflower variety.Here we leveraged an integrative multi-omics strategy by combining genomic,comparative genomics,and tissue-specific transcriptome profiling with biochemical analysis to identify uridine diphosphate glycosyltransferases(UGTs)for flavonoid glycoside biosynthesis in safflower.We assembled and annotated a high-quality reference genome of a medicinal safflower variety,‘Yunhong3’.A comprehensive comparative genomic analysis indicated that an evolutionary whole-genome triplication event occurring in safflower contributed to gene amplification of the flavonoid biosynthetic pathway.By combining comparative transcriptome profiling with enzymatic reactions,we identified 11 novel UGTs that could catalyze the conversion of naringenin chalcone and phloretin to the corresponding O-glycosides.Moreover,we outlined the molecular pathway of hydroxysafflor yellow A(HSYA)biosynthesis featured by 17 newly identified UGTs with promising catalytic activity,laying the foundation for the synthetic production of HSYA.Our study reports systemic genome and gene expression information for flavonoid glycoside biosynthesis in medicinal safflower and provides insights into mechanisms regulating HSYA biosynthesis,which would facilitate the genetic improvement and synthetic bioengineering design for producing clinically valuable flavonoid glycosides in safflower.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB27040203)the National Natural Science Foundation of China (No.31770155)
文摘Epigenetics refers to the study of heritable changes in gene function that do not involve changes in the DNA sequence. Such effects on cellular and physiological phenotypic traits may result from external or environmental factors or be part of normal developmental program. In eukaryotes, DNA wraps on a histone octamer(two copies of H2A, H2B, H3 and H4) to form nucleosome, the fundamental unit of chromatin. The structure of chromatin is subjected to a dynamic regulation through multiple epigenetic mechanisms, including DNA methylation, histone posttranslational modifications(PTMs), chromatin remodeling and noncoding RNAs. As conserved regulatory mechanisms in gene expression, epigenetic mechanisms participate in almost all the important biological processes ranging from basal development to environmental response. Importantly, all of the major epigenetic mechanisms in mammalians also occur in plants. Plant studies have provided numerous important contributions to the epigenetic research. For example, gene imprinting, a mechanism of parental allele-specific gene expression, was firstly observed in maize; evidence of paramutation, an epigenetic phenomenon that one allele acts in a single locus to induce a heritable change in the other allele, was firstly reported in maize and tomato.Moreover, some unique epigenetic mechanisms have been evolved in plants. For example, the 24-nt siRNA-involved RNA-directed DNA methylation(RdDM) pathway is plant-specific because of the involvements of two plant-specific DNA-dependent RNA polymerases, Pol IV and Pol V. A thorough study of epigenetic mechanisms is of great significance to improve crop agronomic traits and environmental adaptability. In this review, we make a brief summary of important progress achieved in plant epigenetics field in China over the past several decades and give a brief outlook on future research prospects.We focus our review on DNA methylation and histone PTMs, the two most important aspects of epigenetic mechanisms.
基金supported by University Grants Commission, New Delhi,India
文摘Nonalcoholic fatty liver disease （NAFLD） is the most common liver disease in the world today. Its incidence in adults and children is rising rapidly due to the ongoing epidemics of obesity and type 2 diabetes. Hence, it has become a global public health issue. Envi- ronmental factors have been found to play a major role in the etiology of NAFLD, especially for genetically susceptible populations. Among these, one of the most important factors is junk food, especially the typical ＂Western-style＂ diet rich in simple carbohydrates, saturated fat, and highly processed food materials. Genetic predisposition to NAFLD does occur; however, a precise definition of genetic factors responsible for NAFLD is still lacking. Specific variants of different genes have been shown to present a risk for NAFLD. Genetic studies might be helpful in the management of the disease by developing novel treatment strategies based on individual＇s genotype.
基金supported by the grants from the National Natural Science Foundation of China (Nos.31071083 and 31130033)the National Science Foundation of USA (No.DBI 0922703)
文摘Plant centromeres are generally composed of tandem arrays of simple repeats that form a complex chromosome locus where the kinetochore forms and microtubules attach during mitosis and meiosis. Each chromosome has one centromere region, which is essential for accurate division of the genetic material. Recently, chromosomes containing two centromere regions （called dicentric chromosomes） have been found in maize and wheat. Interestingly, some dicentric chromosomes are stable because only one centromere is active and the other one is inactivated. Because such arrays maintain their typical structure for both active and inactive centromeres, the specification of centromere activity has an epigenetic component independent of the DNA sequence. Under some circumstances, the inactive centromeres may recover centromere function, which is called centromere reactivation. Recent studies have highlighted the important changes, such as DNA methylation and histone modification, that occur during centromere inactivation and reactivation.
文摘The structure and shape of a cotton shrub aredetermined in result of combining theirelements:length and amount internodes of amain stem and fruit brunches,amount and shapeof leaf blades.Some lines of genetic collectionhave been crossed between each other for
文摘Journal of Genetics and Genomics(JGG),launched in 1974,celebrates its 50th birthday in 2023.With continuous support from our authors,reviewers,readers,and the editorial board,JGG has made remarkable progress in the past year by publishing 122 papers covering major disciplines in life sciences and medical genetics with exciting discoveries.It is worthwhile to note that we have recruited 66 young talents to join JGG as junior editors,resulting in significant improvement in manuscript evaluation and journal promotion.Here,we summarize major progresses reported in JGG in 2023 fromaneditorial view.
文摘This erratum clarifies information in the article“Nucleolar histone deacetylases HDT1,HDT2,and HDT3 regulate plant reproductive development”by Luo et al.(2021).In the section on“Phenotype of hdt mutants”,the T-DNA insertional knockdown mutant for HDT2(hdt2,salk_1247_A02)should be“Sail_1247_A02”in the main text and Fig.3A.
文摘This erratum clarifies information in the Letter to the Editor“Actin polymerization induces mitochondrial distribution during collective cell migration”by Qu et al.(2023).In the section for the list of author names,“Chen Qu,Yating Kan,Hui Zuo,Mengqi Wu,Zhixiang Dong,Xinyi Wang,Qing Zhang,Heng Wang,Dou Wang,Jiong Chen”should be“Chen Qu,Yating Kan,Xinyi Wang,Hui Zuo,Mengqi Wu,Zhixiang Dong,Qing Zhang,Heng Wang,Dou Wang,Jiong Chen”.
文摘<p style="text-align:justify;"> <span style="font-family:""><span style="font-family:Verdana;">In the article, the first time genes of anthocyanin pigmentation R</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;"> and </span></span><span style="font-family:""><span style="font-family:Verdana;">R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span><span style="font-family:Verdana;"> appeared in cotton </span><span style="font-family:Verdana;">plant</span><span style="font-family:Verdana;"> interacting complementarily. Recessive homozygosis r</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">r</span></span></span><sub><span style="font-family:Verdana;">р</span></sub><span style="font-family:""><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span><span style="font-family:Verdana;"> conditions the development of green </span><span style="font-family:Verdana;">colour</span><span style="font-family:Verdana;">. The gen </span></span><span style="font-family:Verdana;">R</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;"> conditions the development of anthocyanin in all organs of plants, </span><span style="font-family:Verdana;">R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span><span style="font-family:Verdana;"> only exists on the stalk </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> in the nerves of leaves, the flower is uncolored, the boll is green. The plants’ existence in </span><span style="font-family:Verdana;">genotype</span><span style="font-family:Verdana;"> of both dominant </span><span style="font-family:Verdana;">equilocal</span><span style="font-family:Verdana;"> genes (R</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">, R</span><sub><span style="font-family:Verdana;">st</span></sub><span style="font-family:Verdana;">) provides </span></span></span><span style="font-family:Verdana;">a </span><span style="font-family:Verdana;">high</span><span style="font-family:Verdana;"> rate of biosynthesis of anthocyanin</span><span style="font-family:""><span style="font-family:Verdana;">—</span><span style="font-family:Verdana;">dark red. </span></span><span style="font-family:Verdana;">Inheritance of the chlorophylls “a” + </span><span style="font-family:Verdana;">“</span><span style="font-family:""><span style="font-family:Verdana;">b” and “a” and “b” quantity in anthocyanin </span><span style="font-family:Verdana;">coloured</span><span style="font-family:Verdana;"> L-2 and L-3 lines of F</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;"> and F</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> generations. The </span><span style="font-family:Verdana;">quantity</span><span style="font-family:Verdana;"> of chlorophylls in anthocyanin </span><span style="font-family:Verdana;">coloured</span><span style="font-family:Verdana;"> L-3 line was defined 1.0</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">1.5 times higher and participation of the gen R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;"> responsible for anthocyanin </span><span style="font-family:Verdana;">colour</span><span style="font-family:Verdana;"> in chlorophylls biosynthesis was also defined. Apparently, in </span><span style="font-family:Verdana;">the line</span><span style="font-family:Verdana;"> L-3 in R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;"> is localized</span></span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> there are gens </span><span style="font-family:Verdana;">which are</span><span style="font-family:Verdana;"> responsible for the quantity of chlorophyll or R</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;"> has </span><span style="font-family:Verdana;">pleiotropic</span><span style="font-family:Verdana;"> effect</span></span><span style="font-family:Verdana;">s</span><span style="font-family:""><span style="font-family:Verdana;"> on the quantity of chlorophyll. It is proved with </span><span style="font-family:Verdana;">low</span><span style="font-family:Verdana;"> quantity of chlorophyll in green </span><span style="font-family:Verdana;">coloured</span><span style="font-family:Verdana;"> plants by recessive homozygote condition </span><span><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">p</span></sub><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup><span style="font-family:Verdana;">r</span><sub><span style="font-family:Verdana;">st</span></sub><sup><span style="font-family:Verdana;">v</span></sup></span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">green </span><span style="font-family:Verdana;">colour</span><span style="font-family:Verdana;">.</span></span> </p>
基金financially supported by National Natural Science Foundation of China(32301800,32301923 and 32072053)Wheat Industrial Technology System of Shandong Province(SDAIT-01-01)Key Research and Development Project of Shandong Province(2022LZG002-4,2023LZGC009-4-4).
文摘Powdery mildew negatively impacts wheat yield and quality.Emmer wheat(Triticum dicoccum),an ancestral species of common wheat,is a gene donor for wheat improvement.Cultivated emmer accession H1-707 exhibited all-stage resistance to powdery mildew over consecutive years.Genetic analysis of H1-707 at the seedling stage revealed a dominant monogenic inheritance pattern,and the underlying gene was designated Pm71.By employing bulked segregant exome sequencing(BSE-Seq)and using 2000 F2:3 families,Pm71 was fine mapped to a 336-kb interval on chromosome arm 6AS by referencing to the durum cv.Svevo RefSeq 1.0.Collinearity analysis revealed high homology in the candidate interval between Svevo and six Triticum species.Among six high-confidence genes annotated within this interval,TRITD6Av1G005050 encoding a GDSL esterase/lipase was identified as a key candidate for Pm71.
基金funded by grants from the National Natural Science Foundation of China (31930006 to Y.W.)the National Key Research and Development Program of China (2022YFF1002903 to Y.W.)+1 种基金the Top Talents Program “One Case One Discussion”(Yishiyiyi to Y.W.)from Shandong provinceShandong Agricultural University Talent Introduction Start-up Fund (to N.Z.)
文摘Branching is a critical aspect of plant architecture that significantly impacts the yield and adaptability of staple cereal crops like rice and wheat.Cereal crops develop tillers during the vegetative stage and panicle or spike branches during the reproductive stage,respectively,both of which are significantly impacted by hormones and genetic factors.Tillering and panicle branching are closely interconnected and exhibit high environmental plasticity.Here,we summarize the recent progress in genetic,hormonal,and environmental factors regulation in the branching of rice and wheat.This review not only provides a comprehensive overview of the current knowledge on branching mechanisms in rice and wheat,but also explores the prospects for future research aimed at optimizing crop architecture for enhanced productivity.
文摘As a result of long standing investigation onurgent problems of amphidiploid cotton speciesGossypium hirsutum genetics,for the first timegenetic determination of the most importanttraits has been established,and the uniquegenetic collection of homozygous isogenic lineshas been developed.A scientifically well-founded theory about combined types ofpolygene interaction in genetic determination oftracts in cotton,has been developed.
基金supported by grant from the National Natural Science Foundation of China(32330056)。
文摘Originally extracted from willow bark,salicylic acid(SA)provided the structural basis for the synthesis of acetylsalicylic acid(aspirin)in 1897,a milestone that exemplifies the far-reaching biomedical relevance of plant-derived metabolites(Desborough and Keeling,2017).In plants,SA functions as a pleiotropic phytohormone that orchestrates immune reprogramming,serving as a central mediator of both local defense responses and systemic acquired resistance(SAR).
基金supported by grants from the National Natural Science Foundation of China(32170312,31830017,and 32200256)State Key Laboratory of Plant Genomics(SKLPG2023-22).
文摘Reactive oxygen species(ROS)and nitric oxide(NO)are two critical classes of signaling molecules that regulate plant development and stress responses.The intracellular level of S-nitrosoglutathione(GSNO),a major bioactive NO species,is regulated by the highly conserved GSNO reductase(GSNOR).However,the molecular mechanisms underlying ROS-mediated regulation of GSNOR remain largely unclear.Here,we show that H_(2)O_(2)negatively regulates the activity of GSNOR1 during ovule development in Arabidopsis.S-sulfenylation of GSNOR1 at Cys-284 inhibits its enzymatic activity.A GSNOR1C284S mutation causes a reduction of the total SNO level in pistils,thereby disrupting NO homeostasis and eventually leading to defective ovule development.These findings illustrate a unique mechanism by which ROS regulates ovule development through S-sulfenylation-mediated inhibition of the GSNOR activity,thereby establishing a molecular link between ROS and NO signaling pathways in reproductive development.
基金supported by the National Natural Science Foundation of China(Grant No.32172514).
文摘In grapevine,previous studies have suggested that,gibberellin(GA)inhibits bud break before dormancy release while enhanced after dormancy release;the capacity of GA accumulation shows a trend of first inhibition and then upregulation.However,the regulatory mechanism of GA metabolism genes expression is as yet unclear during the process of dormancy release.In this study,we further validated the effect of GA3 and hydrogen cyanamide(HC)on bud break of‘Red Globe’grape,confirmed inhibition and promotion effect,respectively.Restricted GA biosynthetic genes'expression and enhanced GA catabolic gene's expression were observed in the early stage after HC treatment,while opposite expression trend showed in the late stage.VvSVP1,a MADS-box transcription factor gene,was downregulated in the late stage,which might play an important role in regulating GA metabolism genes'expression.It was shown that,VvSVP1 could bind to the promoter regions of GA biosynthetic gene VvGA20ox6 and catabolic gene VvGA2ox3,negatively and positively regulated the corresponding genes'expression,respectively;the contents of GAs related to GA20ox were significantly reduced in the grape callus overexpressed VvSVP1,while the ratio of GAs related to GA2ox were significantly increased.Taken together,VvSVP1 can regulate the endogenous GAs level by manipulating the expression of GA metabolism genes before dormant bud break induced by HC.Our findings may provide some new theoretical insights for the study of bud dormancy regulation in the perennial woody fruit trees.
基金supported by the National Natural Science Foundation of China(Grant Nos.32130096 and 32172667)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.Y2022QC14).
文摘Sulfate transporters(SULTRs)facilitate sulfate uptake and transport in plants.In plants,SULTRs can be classified into four distinct functional groups,among which SULTR3 members are the least characterized,and their functions have not yet been confirmed,especially for SULTR3 in rice.In this study,we analyzed the expression patterns,subcellular localization,and inorganic phosphate(Pi)transporter activity of SULTR3 proteins in yeast.Except for OsSULTR3.4,which localized to the plasma membrane,other OsSULTR3 members were localized to the endoplasmic reticulum(ER)membrane in rice protoplast cells.
基金supported by the National Natural Science Foundation of China(No.41930651 and 41807013)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2020102)。
文摘Vegetation response to the combined effects of human and nature has raised extensive controversy.The relative contributions of ecological restoration and multiple climate factors on vegetation variation remain unclear.Combining spatial and temporal changes in fractional vegetation cover(FVC)and net primary productivity(NPP)from 2000 to 2021,we assessed the trend of these two indicators and quantified the impact of ecological restoration and climate factors on vegetation variation using partial correlation and residual analysis.The results indicated that:(1)From 2000 to 2021,vegetation restoration in the Taihang Mountain area was notably successful.FVC increased by 5.71%over 22 years with a change trend of 0.0018 per annum.The cumulative growth rate of NPP over the same period was 42.03%,corresponding to an average annual increase of 5.492 g(C)·m^(-2)·a^(-1).(2)During the 22-year period,the contribution rates of ecological engineering to FVC and NPP were 65.13%and 64.33%,respectively.Ecological restoration was the dominant factor affecting vegetation change,and its impact was increasing in over 90%of the regional projects.The primary climate controlling factor of NPP was precipitation,while FVC responded synergistically to precipitation(primary)and temperature(secondary).Overall,the impact of precipitation on vegetation was 1.5 times that of temperature.(3)Among various ecological restoration types,grassland restoration contributed the most to vegetation change,followed by forest land restoration.This study offers valuable scientific insights that can guide the sustainability of vegetation ecology in semi-arid mountainous areas.
基金National Natural Science Foundation of China,No.41930865Project for Innovative Capacity Improvement in Hebei Province,No.225A4201D。
文摘Nitrate(NO_(3)^(-))accumulation and transport processes in the thick vadose zone affect the evolution of the groundwater NO_(3)^(-)content in intensive agricultural regions.Agricultural land-use change(ALUC),typically accompanied by substantial alterations in nitrogen fertilizer application and irrigation practices,is an important influencing factor.This study evaluated the changes in NO_(3)^(-)accumulation and transport in the deep vadose zone(DVZ,below the root zone),and the groundwater NO_(3)^(-)content associated with ALUC from grain to vegetable fields in the North China Plain(NCP).The ALUC from grain to vegetable resulted in nitrate–nitrogen(NO_(3)^(-)-N)accumulation in DVZ increased by 235.5 kg ha^(-1)m^(-1)(163.2%)in the piedmont plain and 224.9 kg ha^(-1)m^(-1)(102.7%)in the central plain,respectively.This change accelerated downward transport velocity in the DVZ(from 0.81±0.47 to 0.89±0.55 m yr^(-1)in the piedmont plain,and from 0.24±0.12 to 0.92±0.12 m yr^(-1)in the central plain)and increased NO_(3)^(-)leaching fluxes.High transport velocity and leaching fluxes resulted in chemical N-fertilizer entering the aquifer in several areas in the piedmont plain.The impact of the agricultural activity intensity changes,accompanied by the ALUC,on groundwater quantity and quality should be considered in similar regions.
基金financially supported by the National Key R&D Program of China(No.2022YFD1500502)the National Natural Science Foundation of China(No.42277347)+1 种基金the Development Program of the Inner Mongolia Autonomous Region,China(No.NMKJXM202401-01)China Agriculture Research System of Ministry of Finance and Ministry of Agriculture and Rural Affairs(No.CARS–03).
文摘Crop productivity may be affected by soil salinization from brackish water irrigation.Plants host species-specific soil microorganisms that can enhance plant adaptability to salt stress.However,it is unclear whether these specific microorganisms recruited under brackish water irrigation are related to microbial resistance,which has been proven to promote crop production.A field experiment was conducted using five local wheat varieties,each of which was exposed to brackish or fresh water irrigation for four years.Brackish water irrigation tended to increase wheat yield by 6.38%–19.40%,although the increase in yield under brackish water relative to fresh water irrigation varied with wheat variety.The compositions of the bacterial and fungal communities in the bulk soil and rhizosphere were measured,and the taxa enriched following brackish water irrigation were isolated to investigate microbial resistance.We found that the resistance of each wheat variety was determined by specifically recruited microbial taxa with relevant functions.The yield incremental rates were positively regulated by microbial resistance from the bulk soil and rhizosphere.Moreover,the resistance from the bulk soil and rhizosphere had similar effects,and microbial consortia containing both beneficial and harmful taxa determined microbial resistance.These results indicated that brackish water irrigation-induced recruitment of specific microbial taxa from either the bulk soil or the rhizosphere or both positively contributed to the yield incremental rate and provided a list of key taxa linked to the magnitude of yield variation caused by brackish water irrigation.
