Casein andα-lactalbumin(α-LA)are the main allergens in cow's milk,which can affect the skin,respiratory system,and gastrointestinal tract,even cause anaphylactic shock.Developing a rapid and sensitive detection ...Casein andα-lactalbumin(α-LA)are the main allergens in cow's milk,which can affect the skin,respiratory system,and gastrointestinal tract,even cause anaphylactic shock.Developing a rapid and sensitive detection method of casein andα-LA is still pursued.Herein,a surface-enhanced Raman scattering based lateral flow assay(SERS-LFA)method for rapid and highly sensitive detection of milk allergens in food was established for effectively preventing allergic symptoms.Gold@silver nanoparticles(Au@Ag NPs)were synthesized as SERS active substrate to prepare the antibody-modified SERS Probe and SERS-LFA strips toward casein andα-LA were assembled according to the sandwich mode.The detection results were calculated according to colorimetric and Raman signal.The introduction of SRES signal significantly increased the sensitivity of detection with the limit of detection(LOD)of 0.19 ng/mL and 1.74 pg/mL,and exhibited an excellent linear relationship within the range of 0.55-791.50 ng/mL and 0.1 pg/mL-100.0 ng/mL for casein andα-LA,respectively.Furthermore,SERS-LFA strips was highly specific with the recovery rates for corresponding80.36%-105.12%and 85.73%-118.22%for casein andα-LA,respectively.Therefore,the SERS-LFA could be in great potential to develop a unique allergen detection method.展开更多
Oil-in-water(o/w) emulsions were produced with a membrane emulsification system. The effect of the continuous phase viscosity on the emulsification was studied. The theoretical analyses show that the continuous phase ...Oil-in-water(o/w) emulsions were produced with a membrane emulsification system. The effect of the continuous phase viscosity on the emulsification was studied. The theoretical analyses show that the continuous phase viscosity influences not only the flow field of the continuous phase but also the interfacial tension. The droplet size distribution and disperse phase flux for different continuous phase viscosity were investigated experimentally at constant wall shear stress and constant volume flow rate of the continuous phase respectively.展开更多
The growing demand for plant-based proteins has positioned lupin(Lupinus spp.)as a promising alternative to soy.However,bioactive phytochemicals in lupin seeds-although offering valorisation potential-pose chal-lenges...The growing demand for plant-based proteins has positioned lupin(Lupinus spp.)as a promising alternative to soy.However,bioactive phytochemicals in lupin seeds-although offering valorisation potential-pose chal-lenges due to their antinutritional effects and impact on protein functionality.This study investigated how ethanol concentration(EtOH),solvent-to-solute ratio(SSR),and fast heat treatment(HT)using a microwave oven influence the mass yield and composition of lupin protein isolate(LPI),as well as the recovery of phytochemicals in the extraction solvent(ES),using response surface methodology(RSM).Analysis of variance revealed that EtOH and SSR were the most significant factors.The maximum LPI yield was obtained at 11%EtOH and a 23:1 SSR.Increasing EtOH up to 23% increased protein content but caused a linear decrease in phytic acid and total phenolic content(TPC)and a linear increase in saponins in LPI,with opposite trends observed in the ES.Increasing SSR reduced LPI protein and phytochemical contents,except for phytic acid,which increased.Conversely,alkaloids,saponins and TPC generally increased with higher SSR in the ES.The addition of phytase during extraction significantly reduced phytic acid levels,improved LPI solubility,and the stability of its emulsifying and foaming properties.However,phytase treatment slightly reduced protein yield and LPI protein content,with changes being statistically significant but of limited practical relevance.These findings demonstrate the importance of tailoring extraction strategies to balance protein yield,content,and phytochemical management,enabling the production of LPI ingredients while facilitating the valorisation of phytochemical-rich side streams.展开更多
Different tests were carried out to assess the efficiency of replacing plant protection products used at the main application points in citrus fruit packinghouses with natural treatments. The efficiency of an aqueous ...Different tests were carried out to assess the efficiency of replacing plant protection products used at the main application points in citrus fruit packinghouses with natural treatments. The efficiency of an aqueous treatment containing potassium sorbate was found to significantly reduce decaying in Marisol mandarins previously inoculated with Penicillium digitatum and Penicillium italicum. The same food preservative was used in conjunction with a fruit detergent and was applied on Washington Navel oranges and the reduction in the appearance of decaying was similar to that obtained using Guazatine. Finally, two different essential oils were tested. One was combined with a coating and the KS, whilst the other was applied as a fumigant. In the case of wax treatments, it was found that the combination of essential oil or KS with Imazalil at half the dose （0.1%） was equivalent to treatment with a higher dose of Imazalil （0.2%） thus making it possible to reduce the dose of plant protection products used without reducing decay control effectiveness. The treatment with a fumigant with essential oil has a level of efficiency similar to Imazalil against PI. These treatments can be considered a viable alternative to the use of plant protection products.展开更多
During orange juice production, a half of fresh oranges weight is considered as production waste (peels, pulp, seeds, orange leaves and damaged orange fruits). An alternative for the management of these wastes is thei...During orange juice production, a half of fresh oranges weight is considered as production waste (peels, pulp, seeds, orange leaves and damaged orange fruits). An alternative for the management of these wastes is their treatment by addition of lime and a latter pressing, obtaining a press cake and a press liquor rich in sugars (10°Brix) and citric acid, protein, pectin and ethanol. For non-thermal concentration of press liquor to obtain citruss molasses (65°-70°Brix), the removal of pectin is necessary. Traditionally, depectinization of juices has been done by using pectinmethylesterase (PME) enzymes from external sources. In this work it performed the extraction of PME enzymes from orange peels to obtain the optimum extraction conditions. Two different methods of solventextraction were compared (conventional andultrasound-assisted methods). For the conventional extraction experiments, a central composite design with three variables ([NaCl], pH and time) and five replicates of the center point was used. For ultrasound-assisted extraction, experiments were done at pH = 5.5 and [NaCl] = 1.25M), varying extraction time (1-30 min). Response variables were PME activity, protein content and a ratio between them, named PME effectiveness (ηPME). At the same experimental conditions (pH =5.5, [NaCl] = 1.25 M, t = 15 min) it was found that conventional extractions led to slightly better results in terms of ηPME than ultrasound-assisted extraction method.展开更多
During textile manufacturing, huge amounts of wastewaters characterized by removed impurities and high concentrations of dye are produced. These wastewaters cause several problems when they are discharged to the envir...During textile manufacturing, huge amounts of wastewaters characterized by removed impurities and high concentrations of dye are produced. These wastewaters cause several problems when they are discharged to the environment. The use of ozone in wastewater treatment results of interest. In this work we propose to assess the discoloration rate of different synthetic wastewaters as a function of pH, dye concentration (methylene blue (MB)) and reaction time. A comparison of discoloration rate between conventional ozonation and catalytic ozonation salts of copper, zinc, silver and nickel was also performed. For the optimization of the ozonation process of colored solutions, it was used a central composite experimental design with five replicates of the center point resulting to evaluate the influence of the independent variables at different ranges of pH, [MB] and time. In the catalyst-assisted ozonation, [MB], pH and the reaction time were fixed to 100 mg/L, 5.5 and 15 min, respectively. The optimized experimental conditions to provide maximum discoloration were pH=3.3;[MB]=8.6 mg/L and time=74.3 min. Regarding the catalyst-assisted ozonation, it was found that CuSO4 catalyst gave better color reduction if compared with other catalysts assayed.展开更多
Correlation of related factors(texture,gel strength,rheological properties)were analyzed for better characterize 3D material printability.Moreover,printability calculation formula also was established to represent mat...Correlation of related factors(texture,gel strength,rheological properties)were analyzed for better characterize 3D material printability.Moreover,printability calculation formula also was established to represent material plasticity.Pennahia argentata surimi shown best plasticity with 78%moisture content and 20%rice starch respectively.Additionally,starch and carrageenan increased gel strength,yield stress of surimi and starch respectively,which brought better mechanical strength and supporting ability.They also affected viscosity of surimi(increasing)and starch(decreasing),which brought better fluidity and adhesion respectively,resulting in good printability,especially starch(8%)-surimi,carrageenan(0.5%)-starch.Based on their interaction relationships,3D printability equation of printing inkswas established their relevance and corresponding unified values.To judge the printability of same materials under different conditions were consistent with real print results.These could provide methods for judging material printability by specific values and promote the application and spread of 3D materials.展开更多
The food’s perishable properties and people’s eco-friendly requirements have driven the biodegradable active packaging development.In this study,modification behaviors of polyviniyl alcohol/hydroxypropyl methylcellu...The food’s perishable properties and people’s eco-friendly requirements have driven the biodegradable active packaging development.In this study,modification behaviors of polyviniyl alcohol/hydroxypropyl methylcellulose/roselle anthocyanin-based(PHR)film on textural profiles of Penaeus vannamei were investigated.Shrimp samples were packaged by PHR film with different roselle anthocyanin contents and stored at 4±1℃.The results indicated that PHR groups exhibited better texture with higher values of hardness(435.56±75.52 g),springiness(0.53±0.04 mm),chewiness(198.83±40.98 N)and resilience(0.45±0.04).The PHR film exerted positive influences on water migration by reducing drip loss and promoting conversion of free water into immobilized water.Meanwhile,the PHR film retarded protein denaturation via increasing myofibrillar protein and sulfhydryl group contents,decreasing TCA(trichloroacetic acid)-soluble peptide contents and maintaining protein secondary structure(higherα-helix ratio and lowerβ-sheet ratio).In conclusion,the PHR film improved shrimp texture by rearrangement of water distribution and stabilization of protein conformation.Hence,the PHR film was a novel potential active packaging for shrimp texture enhancement.展开更多
The viscosity and plasticity of surimi is remarkable,which is a suitable 3D printing material.But low printing accuracy limit its wide application.Adding xylo-oligosaccharide(XOS)can enhance printing accuracy of surim...The viscosity and plasticity of surimi is remarkable,which is a suitable 3D printing material.But low printing accuracy limit its wide application.Adding xylo-oligosaccharide(XOS)can enhance printing accuracy of surimi printing ink and improve the self-supporting ability and anti-deformation ability of the printed object.At the same time,XOS can impart antioxidant properties to 3D printed surimi.Additionally,adding 20 mM kg^(-1) of Ca^(2+) to the surimi can significantly improve the printing accuracy.With the addition of Ca^(2+) ,the gel strength and texture of surimi are enhanced,as well as the surimi protein became denser.Moreover,the Ca^(2+) in the“ink”can activate the activity of digestive enzymes which increase the contact of enzymes and surimi.The process of enzymatic hydrolysis is promoted,which improving the release rate and antioxidant properties of XOS.Functional surimi with Ca^(2+) can be used to deliver functional components and enhance the printing effects.展开更多
Surimi is a protein-rich food.The peptides hydrolyzed by surimi have many functional activities and showed strong antioxidant activity in ABTS free radical scavenging experiments,hydroxyl radical scavenging experiment...Surimi is a protein-rich food.The peptides hydrolyzed by surimi have many functional activities and showed strong antioxidant activity in ABTS free radical scavenging experiments,hydroxyl radical scavenging experiments and iron ion reduction experiments.The scavenging rates of ABTS ions,hydroxyl radicals and iron ions were 62.83%,74.81%and 0.035 mg/mL,respectively.3D printing ink was formulated by compounding carrageenan,xanthan gum and surimi peptides.The surimi polypeptide“ink”contains 3%carrageenan,4%xanthan gum and 4 mg/mL surimi polypeptide.This“ink”not only has good printability,but also has stable antioxidant properties.The effect of the peptides on the printed structures can also be observed under an inverted fluorescence microscope.In the in vitro digestion experiment,the surimi polypeptide 3D printing ink added with carrageenan and xanthan gum can release most of the peptides.After in vitro digestion experiments,the antioxidant activity of surimi polypeptides increased.The above experiments have proved that surimi polypeptide is a high-quality raw material for preparing 3D printing ink.展开更多
The aim was assessing macronutrient digestibility of common purees in the context of children with cystic fibrosis(CF)starting complementary feeding.A static in vitro digestion model was set up reproducing the conditi...The aim was assessing macronutrient digestibility of common purees in the context of children with cystic fibrosis(CF)starting complementary feeding.A static in vitro digestion model was set up reproducing the conditions of infants with CF and healthy controls.Egg,chicken,infant formula and infant cereal were subjected to digestion individually and in combination with other ingredients.The resulting digesta was subjected to colonic fermentation with the faecal inoculum of an exclusively breast-fed infant with CF.Results showed overall low proteolysis and lipolysis,with positive interaction between egg and chicken with potato,vegetables,and olive oil.Contrarily,the combination of infant cereals and formula had a negative interaction.Notably,the dose of pancreatic enzymes had no effect on lipolysis.Purees rather than in individual foods impacted colonic microbiota,by reducing Bifidobacterium while increasing Veillonella.This study could guide future dietary recom-mendations for complementary feeding in infants with CF.展开更多
基金supported by the Science and Technology Innovation Action Plan of Shanghai,China(17391901302)the National Key R&D Program of China(2018YFC1604401)。
文摘Casein andα-lactalbumin(α-LA)are the main allergens in cow's milk,which can affect the skin,respiratory system,and gastrointestinal tract,even cause anaphylactic shock.Developing a rapid and sensitive detection method of casein andα-LA is still pursued.Herein,a surface-enhanced Raman scattering based lateral flow assay(SERS-LFA)method for rapid and highly sensitive detection of milk allergens in food was established for effectively preventing allergic symptoms.Gold@silver nanoparticles(Au@Ag NPs)were synthesized as SERS active substrate to prepare the antibody-modified SERS Probe and SERS-LFA strips toward casein andα-LA were assembled according to the sandwich mode.The detection results were calculated according to colorimetric and Raman signal.The introduction of SRES signal significantly increased the sensitivity of detection with the limit of detection(LOD)of 0.19 ng/mL and 1.74 pg/mL,and exhibited an excellent linear relationship within the range of 0.55-791.50 ng/mL and 0.1 pg/mL-100.0 ng/mL for casein andα-LA,respectively.Furthermore,SERS-LFA strips was highly specific with the recovery rates for corresponding80.36%-105.12%and 85.73%-118.22%for casein andα-LA,respectively.Therefore,the SERS-LFA could be in great potential to develop a unique allergen detection method.
基金Supported by the National Natural Science Foundation of China(No.29506050)and DAAD scholarship
文摘Oil-in-water(o/w) emulsions were produced with a membrane emulsification system. The effect of the continuous phase viscosity on the emulsification was studied. The theoretical analyses show that the continuous phase viscosity influences not only the flow field of the continuous phase but also the interfacial tension. The droplet size distribution and disperse phase flux for different continuous phase viscosity were investigated experimentally at constant wall shear stress and constant volume flow rate of the continuous phase respectively.
基金supported by the LUPIPROTECH project,grant PID2020-114422RR-C51funded by Spanish Ministerio de Ciencia,Innovación y Universidades and the Agencia Estatal de Investigación MICIU/AEI/10.13039/501100011033+8 种基金by the European Unionthe CERCA program from the Catalan Government Generalitat de Catalunyathe grant PRE2021-099690funded by the Spanish Ministerio de Ciencia,Innovación y Universidades and the Agencia Estatal de Investigación MCIN/AEI/10.13039/501100011033ESF+the grant FI_B 00093(2022)doctoral contract grant funded by the Catalan Government Generalitat de Catalunyathe grant FPU22/00656 funded by the Spanish Ministerio de Ciencia,Innovación y Universidadesthe Agencia Estatal de Investigación MICIU/AEI/10.13039/501100011033by ESF investing in your future.
文摘The growing demand for plant-based proteins has positioned lupin(Lupinus spp.)as a promising alternative to soy.However,bioactive phytochemicals in lupin seeds-although offering valorisation potential-pose chal-lenges due to their antinutritional effects and impact on protein functionality.This study investigated how ethanol concentration(EtOH),solvent-to-solute ratio(SSR),and fast heat treatment(HT)using a microwave oven influence the mass yield and composition of lupin protein isolate(LPI),as well as the recovery of phytochemicals in the extraction solvent(ES),using response surface methodology(RSM).Analysis of variance revealed that EtOH and SSR were the most significant factors.The maximum LPI yield was obtained at 11%EtOH and a 23:1 SSR.Increasing EtOH up to 23% increased protein content but caused a linear decrease in phytic acid and total phenolic content(TPC)and a linear increase in saponins in LPI,with opposite trends observed in the ES.Increasing SSR reduced LPI protein and phytochemical contents,except for phytic acid,which increased.Conversely,alkaloids,saponins and TPC generally increased with higher SSR in the ES.The addition of phytase during extraction significantly reduced phytic acid levels,improved LPI solubility,and the stability of its emulsifying and foaming properties.However,phytase treatment slightly reduced protein yield and LPI protein content,with changes being statistically significant but of limited practical relevance.These findings demonstrate the importance of tailoring extraction strategies to balance protein yield,content,and phytochemical management,enabling the production of LPI ingredients while facilitating the valorisation of phytochemical-rich side streams.
文摘Different tests were carried out to assess the efficiency of replacing plant protection products used at the main application points in citrus fruit packinghouses with natural treatments. The efficiency of an aqueous treatment containing potassium sorbate was found to significantly reduce decaying in Marisol mandarins previously inoculated with Penicillium digitatum and Penicillium italicum. The same food preservative was used in conjunction with a fruit detergent and was applied on Washington Navel oranges and the reduction in the appearance of decaying was similar to that obtained using Guazatine. Finally, two different essential oils were tested. One was combined with a coating and the KS, whilst the other was applied as a fumigant. In the case of wax treatments, it was found that the combination of essential oil or KS with Imazalil at half the dose （0.1%） was equivalent to treatment with a higher dose of Imazalil （0.2%） thus making it possible to reduce the dose of plant protection products used without reducing decay control effectiveness. The treatment with a fumigant with essential oil has a level of efficiency similar to Imazalil against PI. These treatments can be considered a viable alternative to the use of plant protection products.
文摘During orange juice production, a half of fresh oranges weight is considered as production waste (peels, pulp, seeds, orange leaves and damaged orange fruits). An alternative for the management of these wastes is their treatment by addition of lime and a latter pressing, obtaining a press cake and a press liquor rich in sugars (10°Brix) and citric acid, protein, pectin and ethanol. For non-thermal concentration of press liquor to obtain citruss molasses (65°-70°Brix), the removal of pectin is necessary. Traditionally, depectinization of juices has been done by using pectinmethylesterase (PME) enzymes from external sources. In this work it performed the extraction of PME enzymes from orange peels to obtain the optimum extraction conditions. Two different methods of solventextraction were compared (conventional andultrasound-assisted methods). For the conventional extraction experiments, a central composite design with three variables ([NaCl], pH and time) and five replicates of the center point was used. For ultrasound-assisted extraction, experiments were done at pH = 5.5 and [NaCl] = 1.25M), varying extraction time (1-30 min). Response variables were PME activity, protein content and a ratio between them, named PME effectiveness (ηPME). At the same experimental conditions (pH =5.5, [NaCl] = 1.25 M, t = 15 min) it was found that conventional extractions led to slightly better results in terms of ηPME than ultrasound-assisted extraction method.
文摘During textile manufacturing, huge amounts of wastewaters characterized by removed impurities and high concentrations of dye are produced. These wastewaters cause several problems when they are discharged to the environment. The use of ozone in wastewater treatment results of interest. In this work we propose to assess the discoloration rate of different synthetic wastewaters as a function of pH, dye concentration (methylene blue (MB)) and reaction time. A comparison of discoloration rate between conventional ozonation and catalytic ozonation salts of copper, zinc, silver and nickel was also performed. For the optimization of the ozonation process of colored solutions, it was used a central composite experimental design with five replicates of the center point resulting to evaluate the influence of the independent variables at different ranges of pH, [MB] and time. In the catalyst-assisted ozonation, [MB], pH and the reaction time were fixed to 100 mg/L, 5.5 and 15 min, respectively. The optimized experimental conditions to provide maximum discoloration were pH=3.3;[MB]=8.6 mg/L and time=74.3 min. Regarding the catalyst-assisted ozonation, it was found that CuSO4 catalyst gave better color reduction if compared with other catalysts assayed.
基金support of National Science Funding of China(31871868)National Science of Hainan province(321CXTD1012)+1 种基金Scientific Research Foundation of Hainan Tropical Ocean University(RHDRC202117)Japan Society for the Promotion of Science(19H05611).
文摘Correlation of related factors(texture,gel strength,rheological properties)were analyzed for better characterize 3D material printability.Moreover,printability calculation formula also was established to represent material plasticity.Pennahia argentata surimi shown best plasticity with 78%moisture content and 20%rice starch respectively.Additionally,starch and carrageenan increased gel strength,yield stress of surimi and starch respectively,which brought better mechanical strength and supporting ability.They also affected viscosity of surimi(increasing)and starch(decreasing),which brought better fluidity and adhesion respectively,resulting in good printability,especially starch(8%)-surimi,carrageenan(0.5%)-starch.Based on their interaction relationships,3D printability equation of printing inkswas established their relevance and corresponding unified values.To judge the printability of same materials under different conditions were consistent with real print results.These could provide methods for judging material printability by specific values and promote the application and spread of 3D materials.
基金the funding support of Hainan Provincial Natural Science Foundation of China(321CXTD1012)Scientific Research Foundation of Hainan Tropical Ocean University(RHDRC202117)Japan Society for the Promotion of Science(19H05611).
文摘The food’s perishable properties and people’s eco-friendly requirements have driven the biodegradable active packaging development.In this study,modification behaviors of polyviniyl alcohol/hydroxypropyl methylcellulose/roselle anthocyanin-based(PHR)film on textural profiles of Penaeus vannamei were investigated.Shrimp samples were packaged by PHR film with different roselle anthocyanin contents and stored at 4±1℃.The results indicated that PHR groups exhibited better texture with higher values of hardness(435.56±75.52 g),springiness(0.53±0.04 mm),chewiness(198.83±40.98 N)and resilience(0.45±0.04).The PHR film exerted positive influences on water migration by reducing drip loss and promoting conversion of free water into immobilized water.Meanwhile,the PHR film retarded protein denaturation via increasing myofibrillar protein and sulfhydryl group contents,decreasing TCA(trichloroacetic acid)-soluble peptide contents and maintaining protein secondary structure(higherα-helix ratio and lowerβ-sheet ratio).In conclusion,the PHR film improved shrimp texture by rearrangement of water distribution and stabilization of protein conformation.Hence,the PHR film was a novel potential active packaging for shrimp texture enhancement.
基金“National Key R&D Program of China(2018YFD0400600)”“Foundation of Zhejiang Educational Committee(Y201942135)”“Zhejiang Provincial Natural Science Foundation(LQ21C200004)”.
文摘The viscosity and plasticity of surimi is remarkable,which is a suitable 3D printing material.But low printing accuracy limit its wide application.Adding xylo-oligosaccharide(XOS)can enhance printing accuracy of surimi printing ink and improve the self-supporting ability and anti-deformation ability of the printed object.At the same time,XOS can impart antioxidant properties to 3D printed surimi.Additionally,adding 20 mM kg^(-1) of Ca^(2+) to the surimi can significantly improve the printing accuracy.With the addition of Ca^(2+) ,the gel strength and texture of surimi are enhanced,as well as the surimi protein became denser.Moreover,the Ca^(2+) in the“ink”can activate the activity of digestive enzymes which increase the contact of enzymes and surimi.The process of enzymatic hydrolysis is promoted,which improving the release rate and antioxidant properties of XOS.Functional surimi with Ca^(2+) can be used to deliver functional components and enhance the printing effects.
基金funded by“National Key R&D Program of China(2018YFD0400600)”“Foundation of Zhejiang Educational Committee(Y201942135)”“Zhejiang Provincial Natural Science Foundation(LQ21C200004)”.
文摘Surimi is a protein-rich food.The peptides hydrolyzed by surimi have many functional activities and showed strong antioxidant activity in ABTS free radical scavenging experiments,hydroxyl radical scavenging experiments and iron ion reduction experiments.The scavenging rates of ABTS ions,hydroxyl radicals and iron ions were 62.83%,74.81%and 0.035 mg/mL,respectively.3D printing ink was formulated by compounding carrageenan,xanthan gum and surimi peptides.The surimi polypeptide“ink”contains 3%carrageenan,4%xanthan gum and 4 mg/mL surimi polypeptide.This“ink”not only has good printability,but also has stable antioxidant properties.The effect of the peptides on the printed structures can also be observed under an inverted fluorescence microscope.In the in vitro digestion experiment,the surimi polypeptide 3D printing ink added with carrageenan and xanthan gum can release most of the peptides.After in vitro digestion experiments,the antioxidant activity of surimi polypeptides increased.The above experiments have proved that surimi polypeptide is a high-quality raw material for preparing 3D printing ink.
基金funded by Generalitat Valenciana,Conselleria de Innovació,Universitats,Ciència i Societat Digital(Enzimapp Project,ref.AICO/2021/015)Universitat Politècnica de València for the post-doctoral research grant awarded to Andrea Asensio-Grau(PAIDPD-22).
文摘The aim was assessing macronutrient digestibility of common purees in the context of children with cystic fibrosis(CF)starting complementary feeding.A static in vitro digestion model was set up reproducing the conditions of infants with CF and healthy controls.Egg,chicken,infant formula and infant cereal were subjected to digestion individually and in combination with other ingredients.The resulting digesta was subjected to colonic fermentation with the faecal inoculum of an exclusively breast-fed infant with CF.Results showed overall low proteolysis and lipolysis,with positive interaction between egg and chicken with potato,vegetables,and olive oil.Contrarily,the combination of infant cereals and formula had a negative interaction.Notably,the dose of pancreatic enzymes had no effect on lipolysis.Purees rather than in individual foods impacted colonic microbiota,by reducing Bifidobacterium while increasing Veillonella.This study could guide future dietary recom-mendations for complementary feeding in infants with CF.
