Objective The leptin receptor,encoded by the LEPR gene,is involved in tumorigenesis.A potential functional variant of LEPR,rs1137101(Gln223Arg),has been extensively investigated for its contribution to the risk of dig...Objective The leptin receptor,encoded by the LEPR gene,is involved in tumorigenesis.A potential functional variant of LEPR,rs1137101(Gln223Arg),has been extensively investigated for its contribution to the risk of digestive system(DS)cancers,but results remain conflicting rather than conclusive.Here,we performed a case–control study and subsequent meta-analysis to examine the association between rs1137101 and DS cancer risk.Methods A total of 1,727 patients with cancer(gastric/liver/colorectal:460/480/787)and 800 healthy controls were recruited.Genotyping of rs1137101 was conducted using a polymerase chain reactionrestriction fragment length polymorphism(PCR-RFLP)assay and confirmed using Sanger sequencing.Twenty-four eligible studies were included in the meta-analysis.Results After Bonferroni correction,the case–control study revealed that rs1137101 was significantly associated with the risk of liver cancer in the Hubei Chinese population.The meta-analysis suggested that rs1137101 is significantly associated with the risk of overall DS,gastric,and liver cancer in the Chinese population.Conclusion The LEPR rs1137101 variant may be a genetic biomarker for susceptibility to DS cancers(especially liver and gastric cancer)in the Chinese population.展开更多
Cancer is a serious threat to public health and the economy worldwide.Statistical data from the World Health Organization(WHO)in 2018 have demonstrated that lung cancer is the most commonly diagnosed cancer(11.6%of th...Cancer is a serious threat to public health and the economy worldwide.Statistical data from the World Health Organization(WHO)in 2018 have demonstrated that lung cancer is the most commonly diagnosed cancer(11.6%of the total cases)and the leading cause of cancer death(18.4%of the total cancer deaths),closely followed bygastric cancer(5.7%and 8.2%),and liver cancer(4.7%and 8.2%).展开更多
Ribonuclease E(RNase E)is central to bacterial RNA metabolism.In cyanobacteria,its activity is inhibited by RebA,a key mechanism for controlling cell morphology.Here,we demonstrate that rebA is essential for diazotrop...Ribonuclease E(RNase E)is central to bacterial RNA metabolism.In cyanobacteria,its activity is inhibited by RebA,a key mechanism for controlling cell morphology.Here,we demonstrate that rebA is essential for diazotrophic growth of Anabaena PCC 7120,a filamentous cyanobacterium capable of forming heterocysts-specialized nitrogen-fixing cells-upon nitrogen starvation.The rebA mutant strain(ΔrebA)showed severe growth defects in nitrogen-deprived conditions,despite forming more heterocysts than the wild type.With a GFP fusion strain,we show that RebA is transiently upregulated during heterocyst differentiation.Microscopic and ultrastructural analyses revealed thatΔrebA heterocysts accumulated abnormally large cyanophycin granules,while vegetative cells showed reduced pigment levels and disorganized thylakoid membranes,phenotypes indicative of a severe nitrogen deficiency response.However,esculin tracer diffusion and SepJ-GFP localization inΔrebA were comparable to the wild type,suggesting that cell-cell communication via septal junctions remains functional.Thus,the growth defect likely results from impaired degradation or mobilization of fixed nitrogen.Notably,theΔrebA phenotype could be rescued only by wild-type RebA,but not by variants unable to bind RNase E,indicating that RebA's function depends on its modulation of RNase E activity.Together,these findings reveal a key posttranscriptional mechanism linking RNase E regulation to heterocyst development and intercellular nutrient transfer,highlighting the importance of regulated RNA metabolism for diazotrophic growth.展开更多
RNA turnover plays critical roles in the regulation of gene expression and allows cells to respond rapidly to environmental changes.In bacteria,the mechanisms of RNA turnover have been extensively studied in the model...RNA turnover plays critical roles in the regulation of gene expression and allows cells to respond rapidly to environmental changes.In bacteria,the mechanisms of RNA turnover have been extensively studied in the models Escherichia coli and Bacillus subtilis,but not much is known in other bacteria.Cyanobacteria are a diverse group of photosynthetic organisms that have great potential for the sustainable production of valuable products using CO_(2)and solar energy.A better understanding of the regulation of RNA decay is important for both basic and applied studies of cyanobacteria.Genomic analysis shows that cyanobacteria have more than 10 ribonucleases and related proteins in common with E.coli and B.subtilis,and only a limited number of them have been experimentally investigated.In this review,we summarize the current knowledge about these RNAturnover-related proteins in cyanobacteria.Although many of them are biochemically similar to their counterparts in E.coli and B.subtilis,they appear to have distinct cellular functions,suggesting a different mechanism of RNA turnover regulation in cyanobacteria.The identification of new players involved in the regulation of RNA turnover and the elucidation of their biological functions are among the future challenges in this field.展开更多
Ammonia-oxidizing archaea(AOA)play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification.Evaluation of carbon and nitrogen metabolism popularly ...Ammonia-oxidizing archaea(AOA)play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification.Evaluation of carbon and nitrogen metabolism popularly relies on functional genes such as amoA and accA.Increasing studies suggest that quorum sensing(QS)mainly studied in biofilms for bacteria may serve as a universal communication and regulatory mechanism among prokaryotes;however,this has yet to be demonstrated in marine planktonic archaea.To bridge this knowledge gap,we employed a combination of metabolic activity markers(amoA,accA,and grs)to elucidate the regulation of AOA-mediated nitrogen,carbon processes,and their interactions with the sur-rounding heterotrophic population.Through co-transcription investigations linking metabolic markers to potential key QS genes,we discovered that QS molecules could regulate AOA's carbon,nitrogen,and lipid metabolisms under different conditions.Interestingly,specific AOA ecotypes showed a preference for employing distinct QS systems and a distinct QS circuit involving a typical population.Overall,our data demonstrate that Qs orchestrates nitrogen and carbon metabolism,including the exchange of organic metabolites between AOA and surrounding heterotrophic bacteria,which has been pre-viously overlooked in marine AOA research.展开更多
基金supported by the Fundamental Research Funds for the Central Universities(WUT:2020IB029)。
文摘Objective The leptin receptor,encoded by the LEPR gene,is involved in tumorigenesis.A potential functional variant of LEPR,rs1137101(Gln223Arg),has been extensively investigated for its contribution to the risk of digestive system(DS)cancers,but results remain conflicting rather than conclusive.Here,we performed a case–control study and subsequent meta-analysis to examine the association between rs1137101 and DS cancer risk.Methods A total of 1,727 patients with cancer(gastric/liver/colorectal:460/480/787)and 800 healthy controls were recruited.Genotyping of rs1137101 was conducted using a polymerase chain reactionrestriction fragment length polymorphism(PCR-RFLP)assay and confirmed using Sanger sequencing.Twenty-four eligible studies were included in the meta-analysis.Results After Bonferroni correction,the case–control study revealed that rs1137101 was significantly associated with the risk of liver cancer in the Hubei Chinese population.The meta-analysis suggested that rs1137101 is significantly associated with the risk of overall DS,gastric,and liver cancer in the Chinese population.Conclusion The LEPR rs1137101 variant may be a genetic biomarker for susceptibility to DS cancers(especially liver and gastric cancer)in the Chinese population.
基金supported by grants from the Natural Science Foundation of Hubei Province[2019CFB756]the Fundamental Research Funds for the Central Universities[WUT:2020IB029]。
文摘Cancer is a serious threat to public health and the economy worldwide.Statistical data from the World Health Organization(WHO)in 2018 have demonstrated that lung cancer is the most commonly diagnosed cancer(11.6%of the total cases)and the leading cause of cancer death(18.4%of the total cancer deaths),closely followed bygastric cancer(5.7%and 8.2%),and liver cancer(4.7%and 8.2%).
基金supported by the National Natural Science Foundation of China(32070037)the Knowledge Innovation Program of Wuhan-Basic Research(2023020201010087)+1 种基金the China Postdoctoral Science Foundation(2025M772687)the Postdoctoral Fellowship Program of CPSF(GZC20241873)。
文摘Ribonuclease E(RNase E)is central to bacterial RNA metabolism.In cyanobacteria,its activity is inhibited by RebA,a key mechanism for controlling cell morphology.Here,we demonstrate that rebA is essential for diazotrophic growth of Anabaena PCC 7120,a filamentous cyanobacterium capable of forming heterocysts-specialized nitrogen-fixing cells-upon nitrogen starvation.The rebA mutant strain(ΔrebA)showed severe growth defects in nitrogen-deprived conditions,despite forming more heterocysts than the wild type.With a GFP fusion strain,we show that RebA is transiently upregulated during heterocyst differentiation.Microscopic and ultrastructural analyses revealed thatΔrebA heterocysts accumulated abnormally large cyanophycin granules,while vegetative cells showed reduced pigment levels and disorganized thylakoid membranes,phenotypes indicative of a severe nitrogen deficiency response.However,esculin tracer diffusion and SepJ-GFP localization inΔrebA were comparable to the wild type,suggesting that cell-cell communication via septal junctions remains functional.Thus,the growth defect likely results from impaired degradation or mobilization of fixed nitrogen.Notably,theΔrebA phenotype could be rescued only by wild-type RebA,but not by variants unable to bind RNase E,indicating that RebA's function depends on its modulation of RNase E activity.Together,these findings reveal a key posttranscriptional mechanism linking RNase E regulation to heterocyst development and intercellular nutrient transfer,highlighting the importance of regulated RNA metabolism for diazotrophic growth.
基金supported by the National Natural Science Foundation of China(grant No.32070037)the Featured Institute Service Project from the Institute of Hydrobiology,the Chinese Academy of Sciences(grant No.Y85Z061601)the German Science Foundation(DFG)research training group BioInMe 322977937/GRK2344 and grant HE 2544/14-2(to Wolfgang R.Hess).
文摘RNA turnover plays critical roles in the regulation of gene expression and allows cells to respond rapidly to environmental changes.In bacteria,the mechanisms of RNA turnover have been extensively studied in the models Escherichia coli and Bacillus subtilis,but not much is known in other bacteria.Cyanobacteria are a diverse group of photosynthetic organisms that have great potential for the sustainable production of valuable products using CO_(2)and solar energy.A better understanding of the regulation of RNA decay is important for both basic and applied studies of cyanobacteria.Genomic analysis shows that cyanobacteria have more than 10 ribonucleases and related proteins in common with E.coli and B.subtilis,and only a limited number of them have been experimentally investigated.In this review,we summarize the current knowledge about these RNAturnover-related proteins in cyanobacteria.Although many of them are biochemically similar to their counterparts in E.coli and B.subtilis,they appear to have distinct cellular functions,suggesting a different mechanism of RNA turnover regulation in cyanobacteria.The identification of new players involved in the regulation of RNA turnover and the elucidation of their biological functions are among the future challenges in this field.
基金This work was supported by the National Natural Science Foundation of China(Nos.42321004 and 92351301)the Stable Support Plan Program of Shenzhen Natural Science Fund(20200925173954005)+3 种基金the Shenzhen Municipal Commission of Science and Technology Innovation(KCXFZ20211020174803005)the Guangdong Basic and Applied Basic Research Foundation(2021B 1515120080 and 2020B1515120012)the Shenzhen Key Laboratory of Marine Archaea Geo-Omics,Southern University of Science and Technology(ZDSYS201802081843490)the Agence Nationale de la Recherche(ANR)through the projects EUREKA(ANR-14-CE02-0004-01).
文摘Ammonia-oxidizing archaea(AOA)play crucial roles in marine carbon and nitrogen cycles by fixing inorganic carbon and performing the initial step of nitrification.Evaluation of carbon and nitrogen metabolism popularly relies on functional genes such as amoA and accA.Increasing studies suggest that quorum sensing(QS)mainly studied in biofilms for bacteria may serve as a universal communication and regulatory mechanism among prokaryotes;however,this has yet to be demonstrated in marine planktonic archaea.To bridge this knowledge gap,we employed a combination of metabolic activity markers(amoA,accA,and grs)to elucidate the regulation of AOA-mediated nitrogen,carbon processes,and their interactions with the sur-rounding heterotrophic population.Through co-transcription investigations linking metabolic markers to potential key QS genes,we discovered that QS molecules could regulate AOA's carbon,nitrogen,and lipid metabolisms under different conditions.Interestingly,specific AOA ecotypes showed a preference for employing distinct QS systems and a distinct QS circuit involving a typical population.Overall,our data demonstrate that Qs orchestrates nitrogen and carbon metabolism,including the exchange of organic metabolites between AOA and surrounding heterotrophic bacteria,which has been pre-viously overlooked in marine AOA research.
