Objective To investigate the efficacy of rituximab(RTX)monotherapy in the treatment of adult minimal change disease(MCD).Methods This study was a case series analysis.The clinical data of 10 MCD patients who received ...Objective To investigate the efficacy of rituximab(RTX)monotherapy in the treatment of adult minimal change disease(MCD).Methods This study was a case series analysis.The clinical data of 10 MCD patients who received RTX monotherapy at the Department of Nephrology,Zhongda Hospital,Southeast University,from January 2021 to October 2023 were retrospectively analyzed.Results The onset age of the 10 patients was(48.4±21.2)years old,including 7 males and 3 females.Four patients(4/10)were aged 60 or above.One patient(1/10),6 patients(6/10)and 3 patients(3/10)were treated with rituximab once,2 times and 3 times,respectively.The follow-up duration was(327.1±141.6)d.All 10 patients achieved complete remission within 3 months,with 4 cases(4/10)achieving complete remission within 1 month.The mean time to remission was 35.7 d.During the follow-up period,none of the 10 patients experienced relapse.No significant adverse reactions were observed during treatment and follow-up.Conclusion Rituximab monotherapy demonstrates effective treatment outcomes in patients with MCD,but multi-center,large sample clinical validation is required.Long-term follow-up is necessary to assess its sustained efficacy and safety.展开更多
Objective To establish the measurement of IgA1 Oglycan-specific antiglycan autoantibodies in patients withIgA nephropathy (IgAN),and evaluate their role in thedevelopment and progression of IgAN. Methods In theIgAN re...Objective To establish the measurement of IgA1 Oglycan-specific antiglycan autoantibodies in patients withIgA nephropathy (IgAN),and evaluate their role in thedevelopment and progression of IgAN. Methods In theIgAN regular follow-up cohort of Peking University Instituteof Nephrology from January 2006 to December 2015,170 patients drawn by stratified randomization were enrolledin this study. Enzyme-linked immunosorbent assay(ELISA) was used to determine the levels of plasma galactose-deficient IgA1 ( Gd-IgA1 ) and antiglycan autoantibody(IgG and IgA1). The correlation between antiglycanautoantibodies and clinicopathological parameterswas analyzed by linear correlation and multiple linear regressionanalysis. The receiver operating characteristiccurve (ROC) was used to evaluate the value of plasmaanti glycide antibodies in the diagnosis of IgAN.展开更多
Objective To investigate the effects of low density lipoprotein receptor(LDLr)pathway on podocyte injury in diabetic nephropathy(DN)under inflammatory stress.Methods Male db/db mice and db/m mice were randomly divided...Objective To investigate the effects of low density lipoprotein receptor(LDLr)pathway on podocyte injury in diabetic nephropathy(DN)under inflammatory stress.Methods Male db/db mice and db/m mice were randomly divided into four groups(8 mice in each group):db/m group(control),casein injected db/m group(db/m+casein),db/db group(db/db),and casein展开更多
Objective To investigate the effect of active vitamin D(VD)on macrophage M1 and M2 phenotype and its role in protecting podocyte impairment in diabetic nephropathy(DN).Methods Diabetes mellitus rats were established b...Objective To investigate the effect of active vitamin D(VD)on macrophage M1 and M2 phenotype and its role in protecting podocyte impairment in diabetic nephropathy(DN).Methods Diabetes mellitus rats were established by intraperitoneal injection with streptozocin.Rats were randomly divided into four groups:normal-1(NC-1,n=8),normal-2(NC-2,n=8,normal rats treated with calcitriol 0.1μg·kg-1·d-1by gavages),展开更多
Objective To study the mutations of apolipoprotein E (apoE) gene in 4 Chinese lipoprotein glomerulopathy (LPG) patients and their family members, and to investigate the pathogenesis of LPG. Methods Urinalysis was perf...Objective To study the mutations of apolipoprotein E (apoE) gene in 4 Chinese lipoprotein glomerulopathy (LPG) patients and their family members, and to investigate the pathogenesis of LPG. Methods Urinalysis was performed on the family members of two patients, and they were screened for the level of serum creatinine, serum lipid and serum lipoprotein. The mutation of展开更多
Objective To investigate the relationship between intramuscular adipose tissue index(IATI)calculated from computed tomography images at transverse process of the first lumbar and all-cause mortality in maintenance dia...Objective To investigate the relationship between intramuscular adipose tissue index(IATI)calculated from computed tomography images at transverse process of the first lumbar and all-cause mortality in maintenance dialysis patients,and to provide a reference for improving the prognosis in these patients.Methods It was a multicenter retrospective cohort study.The clinical data of patients who received maintenance hemodialysis or peritoneal dialysis treatment from January 1,2017 to December 31,2019 in 4 grade I hospitals including Zhongda Hospital Affiliated to Southeast University,Taizhou People's Hospital Affliated to Nanjing Medical University,Affiliated Hospital of Yangzhou University,and the Third Affiliated Hospital of Soochow University were retrospectively collected.IATI was calculated by low attenuation muscle(LAM)density/skeletal muscle density.The receiver-operating characteristic curve was used to determine the optimal cut-off value of IATI,and the patients were divided into high IATI group and low IATI group according to the optimal cut-off value.The differences of baseline clinical data and measurement parameters of the first lumbar level between the ttwo groups were compared.Thefollow-upended on December 23,2022.展开更多
Objective To investigate whether elevated parathyroid hormone(PTH)levels could induce endothelial-tomesenchymal transition(EndMT)and adipocyte transition in endothelial cells(ECs),and to determine the possible underly...Objective To investigate whether elevated parathyroid hormone(PTH)levels could induce endothelial-tomesenchymal transition(EndMT)and adipocyte transition in endothelial cells(ECs),and to determine the possible underlying mechanism.Methods(1)A rat model of secondary hyperparathyroidism and chronic kidney disease(CKD)was established.The adiposity in bone marrow was detected by oil red O staining.Immunofluorescence staining was performed to detect the expression and localization of cluster of differentiation 31(CD31)and fibroblast-specific protein 1(FSP1).(2)The human umbilical vein ECs were cultured in vitro.Western blotting was performed to detect protein expressions of EndMT-related markers CD31,FSP1 andα-smooth muscle actin(α-SMA)in interference groups with different PTH concentrations(0,10^-11,10^-9,10^-7 mol/L PTH for 48 h)and times(0,12,24,48 h,10^-7 mol/L PTH),as well as the expression ofβ-catenin in interference groups with different PTH concentrations.The localizations of CD31,FSP1 andβ-catenin were observed by cell immunofluorescence.Protein expressions of adipocytes markers peroxisome proliferatoractivated receptor-γ(PPAR-γ)and CCAAT/enhancer binding protein-α(C/EBP-α)by Western blotting and the degree of adipogenesis by oil red O staining were detected after transformed ECs were cultured in adipogenic culture medium for one week.Small interfering RNA(siRNA)was performed to silenceβ-catenin expression.ECs were divided into control siRNA group,β-catenin siRNA group,PTH+control siRNA group and PTH+β-catenin siRNA group.Protein expressions of CD31,FSP1 and PPAR-γby Western blotting and the degree of adipogenesis by oil red O staining were determined.Results(1)In vivo,compared with the control,CKD rats had increased adipocytes in bone marrow(P<0.05),and the co-expression of CD31 and FSP1 in bone marrow ECs.(2)In vitro,PTH significantly inhibited the expression of endothelial marker CD31 and increased the expressions of mesenchymal markers FSP1 andα-SMA in concentration-and time-dependent manners.These indexes in 10-7 mol/L PTH group and 0 mol/L PTH group,in 48 h group and 0 h group showed statistical differences(all P<0.05).In PTH group ECs with 10-7 mol/L PTH for 48 h showed FSP1 accumulation in the cytoplasm and reduced expressions of CD31,and ECs had higher expressions of PPAR-γand C/EBP-αas well as the degree of adipogenesis than those in control group(all P<0.05).Furthermore,PTH enhanced the nuclearβ-catenin protein levels in ECs in concentrationdependent.The expressions ofβ-catenin in 10^-7 mol/L PTH group and 0 mol/L PTH group showed statistical differences(P<0.05).β-catenin expressed in cytoplasm in the control group,while it enter into the nucleus in PTH group.Compared with those in PTH+control siRNA group,the expressions of CD31 and PPAR-γas well as the degree of adipogenesis decreased in PTH+β-catenin siRNA group(all P<0.05),while the expression of FSP1 increased(P<0.05).Conclusion PTH induces ECs-to-adipocytes transition via the canonical Wnt/β-catenin signaling pathway,which might account for bone loss in CKD.Silencedβ-catenin expression can inhibit PTH-induced EndMT and adipogenesis.展开更多
Objective To analyze the efficacy and safety of daratumumab plus dexamethasone in the treatment of renal injury patients with light chain amyloidosis,and to provide clinical reference.Methods It was a single center re...Objective To analyze the efficacy and safety of daratumumab plus dexamethasone in the treatment of renal injury patients with light chain amyloidosis,and to provide clinical reference.Methods It was a single center retrospective observational study.The clinical data before and after daratumumab treatment of renal injury patients with light chain amyloidosis treated with daratumumab plus dexamethasone from December 2021 to August 2022 were retrospectively collected.展开更多
Objective To investigate the effects of CXC chemokine ligand 16(CXCL16)/CXC chemokine receptor 6(CXCR6)pathway on cholesterol accumulation of atherosclerosis in the radial artery of end-stage renal disease(ESRD)patien...Objective To investigate the effects of CXC chemokine ligand 16(CXCL16)/CXC chemokine receptor 6(CXCR6)pathway on cholesterol accumulation of atherosclerosis in the radial artery of end-stage renal disease(ESRD)patients under inflammatory stress and further to investigate its potential mechanisms modulated by purinergic receptor P2X ligandgated ion channel展开更多
Objective To explore the association between the intraperitoneal pressure(IPP)and clinical symptoms in peritoneal dialysis(PD)patients.Methods It was a cross-sectional study.PD patients of Peking University First Hosp...Objective To explore the association between the intraperitoneal pressure(IPP)and clinical symptoms in peritoneal dialysis(PD)patients.Methods It was a cross-sectional study.PD patients of Peking University First Hospital from January 1,to October 31,2021,were enrolled according to the inclusion and exclusion criterion.展开更多
Objective To evaluate the clinicopathological characteristics and long-term outcomes of crescentic IgA nephropathy(crescentic Ig AN)and Henoch-Schonlein purpura nephritis(crescentic HSPN).Methods
文摘Objective To investigate the efficacy of rituximab(RTX)monotherapy in the treatment of adult minimal change disease(MCD).Methods This study was a case series analysis.The clinical data of 10 MCD patients who received RTX monotherapy at the Department of Nephrology,Zhongda Hospital,Southeast University,from January 2021 to October 2023 were retrospectively analyzed.Results The onset age of the 10 patients was(48.4±21.2)years old,including 7 males and 3 females.Four patients(4/10)were aged 60 or above.One patient(1/10),6 patients(6/10)and 3 patients(3/10)were treated with rituximab once,2 times and 3 times,respectively.The follow-up duration was(327.1±141.6)d.All 10 patients achieved complete remission within 3 months,with 4 cases(4/10)achieving complete remission within 1 month.The mean time to remission was 35.7 d.During the follow-up period,none of the 10 patients experienced relapse.No significant adverse reactions were observed during treatment and follow-up.Conclusion Rituximab monotherapy demonstrates effective treatment outcomes in patients with MCD,but multi-center,large sample clinical validation is required.Long-term follow-up is necessary to assess its sustained efficacy and safety.
文摘Objective To establish the measurement of IgA1 Oglycan-specific antiglycan autoantibodies in patients withIgA nephropathy (IgAN),and evaluate their role in thedevelopment and progression of IgAN. Methods In theIgAN regular follow-up cohort of Peking University Instituteof Nephrology from January 2006 to December 2015,170 patients drawn by stratified randomization were enrolledin this study. Enzyme-linked immunosorbent assay(ELISA) was used to determine the levels of plasma galactose-deficient IgA1 ( Gd-IgA1 ) and antiglycan autoantibody(IgG and IgA1). The correlation between antiglycanautoantibodies and clinicopathological parameterswas analyzed by linear correlation and multiple linear regressionanalysis. The receiver operating characteristiccurve (ROC) was used to evaluate the value of plasmaanti glycide antibodies in the diagnosis of IgAN.
文摘Objective To investigate the effects of low density lipoprotein receptor(LDLr)pathway on podocyte injury in diabetic nephropathy(DN)under inflammatory stress.Methods Male db/db mice and db/m mice were randomly divided into four groups(8 mice in each group):db/m group(control),casein injected db/m group(db/m+casein),db/db group(db/db),and casein
文摘Objective To investigate the effect of active vitamin D(VD)on macrophage M1 and M2 phenotype and its role in protecting podocyte impairment in diabetic nephropathy(DN).Methods Diabetes mellitus rats were established by intraperitoneal injection with streptozocin.Rats were randomly divided into four groups:normal-1(NC-1,n=8),normal-2(NC-2,n=8,normal rats treated with calcitriol 0.1μg·kg-1·d-1by gavages),
文摘Objective To study the mutations of apolipoprotein E (apoE) gene in 4 Chinese lipoprotein glomerulopathy (LPG) patients and their family members, and to investigate the pathogenesis of LPG. Methods Urinalysis was performed on the family members of two patients, and they were screened for the level of serum creatinine, serum lipid and serum lipoprotein. The mutation of
文摘Objective To investigate the relationship between intramuscular adipose tissue index(IATI)calculated from computed tomography images at transverse process of the first lumbar and all-cause mortality in maintenance dialysis patients,and to provide a reference for improving the prognosis in these patients.Methods It was a multicenter retrospective cohort study.The clinical data of patients who received maintenance hemodialysis or peritoneal dialysis treatment from January 1,2017 to December 31,2019 in 4 grade I hospitals including Zhongda Hospital Affiliated to Southeast University,Taizhou People's Hospital Affliated to Nanjing Medical University,Affiliated Hospital of Yangzhou University,and the Third Affiliated Hospital of Soochow University were retrospectively collected.IATI was calculated by low attenuation muscle(LAM)density/skeletal muscle density.The receiver-operating characteristic curve was used to determine the optimal cut-off value of IATI,and the patients were divided into high IATI group and low IATI group according to the optimal cut-off value.The differences of baseline clinical data and measurement parameters of the first lumbar level between the ttwo groups were compared.Thefollow-upended on December 23,2022.
文摘Objective To investigate whether elevated parathyroid hormone(PTH)levels could induce endothelial-tomesenchymal transition(EndMT)and adipocyte transition in endothelial cells(ECs),and to determine the possible underlying mechanism.Methods(1)A rat model of secondary hyperparathyroidism and chronic kidney disease(CKD)was established.The adiposity in bone marrow was detected by oil red O staining.Immunofluorescence staining was performed to detect the expression and localization of cluster of differentiation 31(CD31)and fibroblast-specific protein 1(FSP1).(2)The human umbilical vein ECs were cultured in vitro.Western blotting was performed to detect protein expressions of EndMT-related markers CD31,FSP1 andα-smooth muscle actin(α-SMA)in interference groups with different PTH concentrations(0,10^-11,10^-9,10^-7 mol/L PTH for 48 h)and times(0,12,24,48 h,10^-7 mol/L PTH),as well as the expression ofβ-catenin in interference groups with different PTH concentrations.The localizations of CD31,FSP1 andβ-catenin were observed by cell immunofluorescence.Protein expressions of adipocytes markers peroxisome proliferatoractivated receptor-γ(PPAR-γ)and CCAAT/enhancer binding protein-α(C/EBP-α)by Western blotting and the degree of adipogenesis by oil red O staining were detected after transformed ECs were cultured in adipogenic culture medium for one week.Small interfering RNA(siRNA)was performed to silenceβ-catenin expression.ECs were divided into control siRNA group,β-catenin siRNA group,PTH+control siRNA group and PTH+β-catenin siRNA group.Protein expressions of CD31,FSP1 and PPAR-γby Western blotting and the degree of adipogenesis by oil red O staining were determined.Results(1)In vivo,compared with the control,CKD rats had increased adipocytes in bone marrow(P<0.05),and the co-expression of CD31 and FSP1 in bone marrow ECs.(2)In vitro,PTH significantly inhibited the expression of endothelial marker CD31 and increased the expressions of mesenchymal markers FSP1 andα-SMA in concentration-and time-dependent manners.These indexes in 10-7 mol/L PTH group and 0 mol/L PTH group,in 48 h group and 0 h group showed statistical differences(all P<0.05).In PTH group ECs with 10-7 mol/L PTH for 48 h showed FSP1 accumulation in the cytoplasm and reduced expressions of CD31,and ECs had higher expressions of PPAR-γand C/EBP-αas well as the degree of adipogenesis than those in control group(all P<0.05).Furthermore,PTH enhanced the nuclearβ-catenin protein levels in ECs in concentrationdependent.The expressions ofβ-catenin in 10^-7 mol/L PTH group and 0 mol/L PTH group showed statistical differences(P<0.05).β-catenin expressed in cytoplasm in the control group,while it enter into the nucleus in PTH group.Compared with those in PTH+control siRNA group,the expressions of CD31 and PPAR-γas well as the degree of adipogenesis decreased in PTH+β-catenin siRNA group(all P<0.05),while the expression of FSP1 increased(P<0.05).Conclusion PTH induces ECs-to-adipocytes transition via the canonical Wnt/β-catenin signaling pathway,which might account for bone loss in CKD.Silencedβ-catenin expression can inhibit PTH-induced EndMT and adipogenesis.
文摘Objective To analyze the efficacy and safety of daratumumab plus dexamethasone in the treatment of renal injury patients with light chain amyloidosis,and to provide clinical reference.Methods It was a single center retrospective observational study.The clinical data before and after daratumumab treatment of renal injury patients with light chain amyloidosis treated with daratumumab plus dexamethasone from December 2021 to August 2022 were retrospectively collected.
文摘Objective To investigate the effects of CXC chemokine ligand 16(CXCL16)/CXC chemokine receptor 6(CXCR6)pathway on cholesterol accumulation of atherosclerosis in the radial artery of end-stage renal disease(ESRD)patients under inflammatory stress and further to investigate its potential mechanisms modulated by purinergic receptor P2X ligandgated ion channel
文摘Objective To explore the association between the intraperitoneal pressure(IPP)and clinical symptoms in peritoneal dialysis(PD)patients.Methods It was a cross-sectional study.PD patients of Peking University First Hospital from January 1,to October 31,2021,were enrolled according to the inclusion and exclusion criterion.
文摘Objective To evaluate the clinicopathological characteristics and long-term outcomes of crescentic IgA nephropathy(crescentic Ig AN)and Henoch-Schonlein purpura nephritis(crescentic HSPN).Methods
