The absence of control over carriers transport during electrochemical cycling,accompanied by the deterioration of the solid electrolyte interphase(SEI)and the growth of lithium dendrites,has hindered the development o...The absence of control over carriers transport during electrochemical cycling,accompanied by the deterioration of the solid electrolyte interphase(SEI)and the growth of lithium dendrites,has hindered the development of lithium metal batteries.Herein,a separator complexion consisting of polyacrylonitrile(PAN)nanofiber and MIL-101(Cr)particles prepared by electrospinning is proposed to bind the anions from the electrolyte utilizing abundant effective open metal sites in the MIL-101(Cr)particles to modulate the transport of non-effective carriers.The binding effect of the PANM separator promotes uniform lithium metal deposition and enhances the stability of the SEI layer and long cycling stability of ultra-high nickel layered oxide cathodes.Taking PANM as the Li||NCM96 separator enables high-voltage cycling stability,maintaining 72%capacity retention after 800 cycles at a charging and discharging rate of 0.2 C at a cut-off voltage of 4.5 V and 0°C.Meanwhile,the excellent high-rate performance delivers a specific capacity of 156.3 mA h g^(-1) at 10 C.In addition,outstanding cycling performance is realized from−20 to 60°C.The separator engineering facilitates the electrochemical performance of lithium metal batteries and enlightens a facile and promising strategy to develop fast charge/discharge over a wide range of temperatures.展开更多
The specific energy of Li metal batteries(LMBs)can be improved by using high‐voltage cathode materials;however,achieving long‐term stable cycling performance in the corresponding system is particularly challenging f...The specific energy of Li metal batteries(LMBs)can be improved by using high‐voltage cathode materials;however,achieving long‐term stable cycling performance in the corresponding system is particularly challenging for the liquid electrolyte.Herein,a novel pseudo‐oversaturated electrolyte(POSE)is prepared by introducing 1,1,2,2‐tetrafluoroethyl‐2,2,3,3‐tetrafluoropropyl ether(TTE)to adjust the coordination structure between diglyme(G2)and lithium bis(trifluoromethanesulfonyl)imide(LiTFSI).Surprisingly,although TTE shows little solubility to LiTFSI,the molar ratio between LiTFSI and G2 in the POSE can be increased to 1:1,which is much higher than that of the saturation state,1:2.8.Simulation and experimental results prove that TTE promotes closer contact of the G2 molecular with Li^(+)in the POSE.Moreover,it also participates in the formation of electrolyte/electrode interphases.The electrolyte shows outstanding compatibility with both the Li metal anode and typical high‐voltage cathodes.Li||Li symmetric cells show a long life of more than 2000 h at 1 mA cm^(−2),1 mAh cm^(−2).In the meantime,Li||LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)cell with the POSE shows a high reversible capacity of 134.8 mAh g^(−1 )after 900 cycles at 4.5 V,1 C rate.The concept of POSE can provide new insight into the Li^(+)solvation structure and in the design of advanced electrolytes for LMBs.展开更多
The influence of Mo and ZrO_(2)nanoparticles addition on the interfacial properties and shear strength of Sn58Bi solder joint was investigated.The interfacial microstructures of Sn58Bi/Cu,Sn58Bi+Mo/Cu and Sn58Bi+ZrO_(...The influence of Mo and ZrO_(2)nanoparticles addition on the interfacial properties and shear strength of Sn58Bi solder joint was investigated.The interfacial microstructures of Sn58Bi/Cu,Sn58Bi+Mo/Cu and Sn58Bi+ZrO_(2)/Cu solder joints were analysed using a scanning electron microscope(SEM)coupled with energy dispersive X-ray(EDX)and the X-ray diffraction(XRD).Intermetallic compounds(IMCs)of MoSn_(2)are detected in the Sn58Bi+Mo/Cu solder joint,while SnZr,Zr_(5)Sn_(3),ZrCu and ZrSn_(2)are detected in Sn58Bi+ZrO_(2)/Cu solder joint.IMC layers for both composite solders comprise of Cu_(6)Sn_(5) and Cu_(3)Sn.The SEM images of these layers were used to measure the IMC layer’s thickness.The average IMC layer’s thickness is 1.4431μm for Sn58Bi+Mo/Cu and 0.9112μm for Sn58Bi+ZrO_(2)/Cu solder joints.Shear strength of the solder joints was investigated via the single shear lap test method.The average maximum load and shear stress of the Sn58Bi+Mo/Cu and Sn58Bi+ZrO_(2)/Cu solder joints are increased by 33%and 69%,respectively,as compared to those of the Sn58Bi/Cu solder joint.By comparing both composite solder joints,the latter prevails better as adding smaller sized ZrO_(2)nanoparticles improves the interfacial properties granting a stronger solder joint.展开更多
Earth surveillance through aerial images allows more accurate identification and characterization of objects present on the surface from space and airborne platforms.The progression of deep learning and computer visio...Earth surveillance through aerial images allows more accurate identification and characterization of objects present on the surface from space and airborne platforms.The progression of deep learning and computer vision methods and the availability of heterogeneous multispectral remote sensing data make the field more fertile for research.With the evolution of optical sensors,aerial images are becoming more precise and larger,which leads to a new kind of problem for object detection algorithms.This paper proposes the“Sliding Region-based Convolutional Neural Network(SRCNN),”which is an extension of the Faster Region-based Convolutional Neural Network(RCNN)object detection framework to make it independent of the image’s spatial resolution and size.The sliding box strategy is used in the proposed model to segment the image while detecting.The proposed framework outperforms the state-of-the-art Faster RCNN model while processing images with significantly different spatial resolution values.The SRCNN is also capable of detecting objects in images of any size.展开更多
Animal models have been extensively used as a gold standard in various biological research,including immu-nological studies.Despite high availability and ease of handling procedure,they inadequately represent complex ...Animal models have been extensively used as a gold standard in various biological research,including immu-nological studies.Despite high availability and ease of handling procedure,they inadequately represent complex interactions and unique cellular properties in humans due to inter-species genetic and microenvironmental differences which have resulted in clinical-stage failures.Organoid technology has gained enormous attention as they provide sophisticated insights about tissue architecture and functionality in miniaturized organs.In this review,we describe the use of organoid system to overcome limitations in animal-based investigations,such as physiological mismatch with humans,costly,time-consuming,and low throughput screening.Immune organoids are one of the specific advancements in organogenesis ex vivo,which can reflect human adaptive immunity with more physiologically relevant aspects.We discuss how immune organoids are established from patient-derived lymphoid tissues,as well as their characteristics and functional features to understand immune mechanisms and responses.Also,some bioengineering perspectives are considered for any potential progress of immuno-engineered organoids.展开更多
Infectious wound healing remains a significant medical challenge due to chronic inflammation and bacterial colonization.Effective antimicrobial and anti-inflammatory therapies are essential to facilitate wound recover...Infectious wound healing remains a significant medical challenge due to chronic inflammation and bacterial colonization.Effective antimicrobial and anti-inflammatory therapies are essential to facilitate wound recovery.Herein,we introduce a highly biocompatible,ROS-responsive DNA hydrogel(LGAH),modified with aggregationinduced emission luminogens(AIEgen)and incorporating ginseng-derived exosomes(G-Exos)and nitric oxide(NO)donor-L-arginine(L-Arg)to promote healing of infected wounds.The hydrogel degrades in response to elevated ROS levels,releasing therapeutic agents.Upon laser irradiation,AIEgen generates 1O2,which activates L-Arg to produce NO,leading to a synergistic antimicrobial effect.NO is particularly effective at inhibiting bacterial growth and promoting angiogenesis,supporting wound healing.G-Exos modulate immune responses,reduce inflammation,and promote the transition from the inflammatory to the proliferative phase.They also enhance cell proliferation,migration,and collagen production,which are key to tissue regeneration.In vivo experiments demonstrated that LGAH significantly accelerates S.aureus-infected wound healing by modulating the wound microenvironment and promoting tissue regeneration.Transcriptomic analysis revealed that LGAH down-regulates gene expression in inflammation and immune response signaling pathways while up-regulating genes related to energy metabolism.Biosafety evaluations at cellular and animal levels have demonstrated that LGAH possesses excellent biocompatibility and biodegradability,making it ideal for tissue repair and regener-ation.This multifunctional DNA hydrogel system offers a safe and promising strategy for the clinical treatment of infected wounds.展开更多
Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory properties.Conventional cell expansion methods using 2D tissue cultur...Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory properties.Conventional cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in vivo.To overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to therapy.With our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and proliferation.In addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK pathway.With our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.展开更多
Microbial resistance to current antibiotics therapies is a major cause of implant failure and adverse clinical outcomes in orthopaedic surgery.Recent developments in advanced antimicrobial nanotechnologies provide num...Microbial resistance to current antibiotics therapies is a major cause of implant failure and adverse clinical outcomes in orthopaedic surgery.Recent developments in advanced antimicrobial nanotechnologies provide numerous opportunities to effective remove resistant bacteria and prevent resistance from occurring through unique mechanisms.With tunable physicochemical properties,nanomaterials can be designed to be bactericidal,antifouling,immunomodulating,and capable of delivering antibacterial compounds to the infection region with spatiotemporal accuracy.Despite its substantial advancement,an important,but under-explored area,is potential microbial resistance to nanomaterials and how this can impact the clinical use of antimicrobial nanotechnologies.This review aims to provide a better understanding of nanomaterial-associated microbial resistance to accelerate bench-to-bedside translations of emerging nanotechnologies for effective control of implant associated infections.展开更多
The impact of the composition of blends comprising low‐density polyethylene(LDPE),isotactic polypropylene(PP)and a styrenic copolymer additive on the thermomechanical properties as well as the direct‐current(DC)elec...The impact of the composition of blends comprising low‐density polyethylene(LDPE),isotactic polypropylene(PP)and a styrenic copolymer additive on the thermomechanical properties as well as the direct‐current(DC)electrical and thermal conductivity is investigated.The presence of 5 weight percent(wt%)of the styrenic copolymer strongly reduces the amount of PP that is needed to enhance the storage modulus above the melting temperature of LDPE from 40 to 24 wt%.At the same time,the copolymer improves the consistency of the thermomechanical properties of the resulting ternary blends.While both the DC electrical and thermal conductivity strongly decrease with PP content,the addition of the styrenic copolymer appears to have little influence on either property.Evidently,PP in combination with small amounts of a styrenic copolymer not only allows to reinforce LDPE at elevated temperatures but also functions as an electrical conductivity‐reducing additive,which makes such thermoplastic ternary formulations possible candidates for the insulation of high‐voltage power cables.展开更多
Infectious diseases present significant challenges to global health,thereby extensively affecting both human society and the economy.In recent years,functional probes have demonstrated remarkable potential as crucial ...Infectious diseases present significant challenges to global health,thereby extensively affecting both human society and the economy.In recent years,functional probes have demonstrated remarkable potential as crucial biomedical media for the research and treatment of infectious diseases.Their applications in the realm of infectious diseases include pathogen detection,exploration of biological mechanisms,and development of anti-infective drugs.This review provides a concise introduction to the severity,classification,and pathogenesis of infectious diseases.Subsequently,we examined the distinctiveness and design strategies of functional probes for diagnosing and treating infectious diseases,shedding light on their design rationale using typical examples.We discuss the current status and challenges associated with the clinical implementation of functional probes.Furthermore,we explored the prospects of using these probes for the diagnosis and treatment of infectious diseases.This review aims to offer novel insights into the design of diagnostic probes for infectious diseases and broaden their applications in disease treatment.展开更多
Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing performance.In this study,we introduce a novel droplet microfluidic chip to ...Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing performance.In this study,we introduce a novel droplet microfluidic chip to overcome this challenge.The chip comprises a double spiral focusing unit,a flow resistance-based sample enrichment module with fine-tunable outlets,and a crossflow droplet generation unit.Utilizing a low-density cell/bead suspension(2×10^(6) objects/mL),cells/beads are focused into a near-equidistant linear arrangement within the double spiral microchannel.The excess water phase is diverted while cells/beads remain focused and sequentially encapsulated in individual droplets.Focusing performance was assessed through numerical simulations and experiments at three flow rates(40,60,80μL/min),demonstrating successful focusing at 40 and 80μL/min for beads and cells,respectively.In addition,both simulation and experimental results revealed that the flow resistance at the sample enrichment module is adjustable by punching different outlets,allowing over 50%of the aqueous phase to be removed.YOLOv8n-based droplet detection algorithms realized the counting of cells/beads in droplets,statistically demonstrating single-cell and bead encapsulation rates of 72.2%and 79.2%,respectively.All the results indicate that this on-chip sample enrichment approach can be further developed and employed as a critical component in single-cell encapsulation in water-in-oil droplets.展开更多
Microgreens are young,tender greens that are used to enhance the color,texture,or flavor of salads and main dishes.They can be grown in small scales and indoors,making them widely adopted by controlled environment agr...Microgreens are young,tender greens that are used to enhance the color,texture,or flavor of salads and main dishes.They can be grown in small scales and indoors,making them widely adopted by controlled environment agriculture,an indoor farming practice is particularly important for feeding increasing urban populations.Besides,microgreens are attracting more consumers’attention due to their high nutritional value and unique sensory characteristics.This review focuses on the nutrition quality,sensory evaluation,pre-and post-harvest interventions,and health benefits of microgreens.Microgreens are rich in vitamins(e.g.,VC),minerals(e.g.,copper and zinc),and phytochemicals,including carotenoids and phenolic compounds,which act as antioxidants in human body.Pre-harvest interventions,such as illumination,salinity stress,nutrient fortification,and natural substrates,infl uence the photosynthetic and metabolic activities of microgreens and were shown to improve their nutritional quality,while the effects varied among species.After harvesting,packaging method and storage temperature can infl uence the nutrient retention in microgreens.Both in vitro and in vivo studies have shown that microgreens have anti-infl ammatory,anti-cancer,anti-bacterial,and anti-hyperglycemia properties,making it a new functional food beneficial to human health.The sensory attributes and overall acceptability and liking of microgreens are primarily infl uenced by their phytochemical content.Microgreens are only getting popular during the last decades and research on microgreens is still at its early stage.More studies are warranted to optimize the pre-and post-harvest practices for nutrient enhancement and retention and to explore the potential health benefits of different microgreens for the prevention and treatment of chronic diseases.展开更多
基金financially supported by the National Key Research and Development Program of China(No.2021YFB2400300)the IPE Talent Start-up Program of Institute of Process Engineering of Chinese Academy of Sciences(Grant No.E0293507)。
文摘The absence of control over carriers transport during electrochemical cycling,accompanied by the deterioration of the solid electrolyte interphase(SEI)and the growth of lithium dendrites,has hindered the development of lithium metal batteries.Herein,a separator complexion consisting of polyacrylonitrile(PAN)nanofiber and MIL-101(Cr)particles prepared by electrospinning is proposed to bind the anions from the electrolyte utilizing abundant effective open metal sites in the MIL-101(Cr)particles to modulate the transport of non-effective carriers.The binding effect of the PANM separator promotes uniform lithium metal deposition and enhances the stability of the SEI layer and long cycling stability of ultra-high nickel layered oxide cathodes.Taking PANM as the Li||NCM96 separator enables high-voltage cycling stability,maintaining 72%capacity retention after 800 cycles at a charging and discharging rate of 0.2 C at a cut-off voltage of 4.5 V and 0°C.Meanwhile,the excellent high-rate performance delivers a specific capacity of 156.3 mA h g^(-1) at 10 C.In addition,outstanding cycling performance is realized from−20 to 60°C.The separator engineering facilitates the electrochemical performance of lithium metal batteries and enlightens a facile and promising strategy to develop fast charge/discharge over a wide range of temperatures.
基金Subsidy for Hebei Key Laboratory of Applied Chemistry after Operation Performance,Grant/Award Number:22567616HNatural Science Foundation of Hebei Province of China,Grant/Award Number:B2020103028+3 种基金Science Fund for Creative Research Groups of the National Natural Science Foundation of China,Grant/Award Number:21921005National Key Research and Development Program of China,Grant/Award Number:2021YFB2400300Beijing Municipal Natural Science Foundation Project,Grant/Award Number:2222031National Natural Science Foundation of China,Grant/Award Numbers:52174281,21808228。
文摘The specific energy of Li metal batteries(LMBs)can be improved by using high‐voltage cathode materials;however,achieving long‐term stable cycling performance in the corresponding system is particularly challenging for the liquid electrolyte.Herein,a novel pseudo‐oversaturated electrolyte(POSE)is prepared by introducing 1,1,2,2‐tetrafluoroethyl‐2,2,3,3‐tetrafluoropropyl ether(TTE)to adjust the coordination structure between diglyme(G2)and lithium bis(trifluoromethanesulfonyl)imide(LiTFSI).Surprisingly,although TTE shows little solubility to LiTFSI,the molar ratio between LiTFSI and G2 in the POSE can be increased to 1:1,which is much higher than that of the saturation state,1:2.8.Simulation and experimental results prove that TTE promotes closer contact of the G2 molecular with Li^(+)in the POSE.Moreover,it also participates in the formation of electrolyte/electrode interphases.The electrolyte shows outstanding compatibility with both the Li metal anode and typical high‐voltage cathodes.Li||Li symmetric cells show a long life of more than 2000 h at 1 mA cm^(−2),1 mAh cm^(−2).In the meantime,Li||LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)cell with the POSE shows a high reversible capacity of 134.8 mAh g^(−1 )after 900 cycles at 4.5 V,1 C rate.The concept of POSE can provide new insight into the Li^(+)solvation structure and in the design of advanced electrolytes for LMBs.
文摘The influence of Mo and ZrO_(2)nanoparticles addition on the interfacial properties and shear strength of Sn58Bi solder joint was investigated.The interfacial microstructures of Sn58Bi/Cu,Sn58Bi+Mo/Cu and Sn58Bi+ZrO_(2)/Cu solder joints were analysed using a scanning electron microscope(SEM)coupled with energy dispersive X-ray(EDX)and the X-ray diffraction(XRD).Intermetallic compounds(IMCs)of MoSn_(2)are detected in the Sn58Bi+Mo/Cu solder joint,while SnZr,Zr_(5)Sn_(3),ZrCu and ZrSn_(2)are detected in Sn58Bi+ZrO_(2)/Cu solder joint.IMC layers for both composite solders comprise of Cu_(6)Sn_(5) and Cu_(3)Sn.The SEM images of these layers were used to measure the IMC layer’s thickness.The average IMC layer’s thickness is 1.4431μm for Sn58Bi+Mo/Cu and 0.9112μm for Sn58Bi+ZrO_(2)/Cu solder joints.Shear strength of the solder joints was investigated via the single shear lap test method.The average maximum load and shear stress of the Sn58Bi+Mo/Cu and Sn58Bi+ZrO_(2)/Cu solder joints are increased by 33%and 69%,respectively,as compared to those of the Sn58Bi/Cu solder joint.By comparing both composite solder joints,the latter prevails better as adding smaller sized ZrO_(2)nanoparticles improves the interfacial properties granting a stronger solder joint.
文摘Earth surveillance through aerial images allows more accurate identification and characterization of objects present on the surface from space and airborne platforms.The progression of deep learning and computer vision methods and the availability of heterogeneous multispectral remote sensing data make the field more fertile for research.With the evolution of optical sensors,aerial images are becoming more precise and larger,which leads to a new kind of problem for object detection algorithms.This paper proposes the“Sliding Region-based Convolutional Neural Network(SRCNN),”which is an extension of the Faster Region-based Convolutional Neural Network(RCNN)object detection framework to make it independent of the image’s spatial resolution and size.The sliding box strategy is used in the proposed model to segment the image while detecting.The proposed framework outperforms the state-of-the-art Faster RCNN model while processing images with significantly different spatial resolution values.The SRCNN is also capable of detecting objects in images of any size.
基金supported by the NUS Presidential Young Professor-ship,Ministry of Education Tier 1,National Medical Research Council Open Fund Young Investigator Research Grant,Manufacturing,Trade and Connectivity Young Investigator Research Grant,iHT OOE award,NUS Healthy Longevity TRP Aged Mice Funding,Joint NCIS Centre Grant and NUS Centre for Cancer Research(N2CR)Seed Funding Programme,TREX Grant,PCM Seed Grant,and PREPARE Strategic Open Grant Call(Vaccines&Therapeutics Co-Operative Programme).
文摘Animal models have been extensively used as a gold standard in various biological research,including immu-nological studies.Despite high availability and ease of handling procedure,they inadequately represent complex interactions and unique cellular properties in humans due to inter-species genetic and microenvironmental differences which have resulted in clinical-stage failures.Organoid technology has gained enormous attention as they provide sophisticated insights about tissue architecture and functionality in miniaturized organs.In this review,we describe the use of organoid system to overcome limitations in animal-based investigations,such as physiological mismatch with humans,costly,time-consuming,and low throughput screening.Immune organoids are one of the specific advancements in organogenesis ex vivo,which can reflect human adaptive immunity with more physiologically relevant aspects.We discuss how immune organoids are established from patient-derived lymphoid tissues,as well as their characteristics and functional features to understand immune mechanisms and responses.Also,some bioengineering perspectives are considered for any potential progress of immuno-engineered organoids.
基金supported by the National Key R&D Pro-gram of China(2024YFC231103)National Natural Science Foundation of China(82322042,and 82272248)Natural Science Fund of Guangdong Province for Distinguished Young Scholars(2022B1515020089).
文摘Infectious wound healing remains a significant medical challenge due to chronic inflammation and bacterial colonization.Effective antimicrobial and anti-inflammatory therapies are essential to facilitate wound recovery.Herein,we introduce a highly biocompatible,ROS-responsive DNA hydrogel(LGAH),modified with aggregationinduced emission luminogens(AIEgen)and incorporating ginseng-derived exosomes(G-Exos)and nitric oxide(NO)donor-L-arginine(L-Arg)to promote healing of infected wounds.The hydrogel degrades in response to elevated ROS levels,releasing therapeutic agents.Upon laser irradiation,AIEgen generates 1O2,which activates L-Arg to produce NO,leading to a synergistic antimicrobial effect.NO is particularly effective at inhibiting bacterial growth and promoting angiogenesis,supporting wound healing.G-Exos modulate immune responses,reduce inflammation,and promote the transition from the inflammatory to the proliferative phase.They also enhance cell proliferation,migration,and collagen production,which are key to tissue regeneration.In vivo experiments demonstrated that LGAH significantly accelerates S.aureus-infected wound healing by modulating the wound microenvironment and promoting tissue regeneration.Transcriptomic analysis revealed that LGAH down-regulates gene expression in inflammation and immune response signaling pathways while up-regulating genes related to energy metabolism.Biosafety evaluations at cellular and animal levels have demonstrated that LGAH possesses excellent biocompatibility and biodegradability,making it ideal for tissue repair and regener-ation.This multifunctional DNA hydrogel system offers a safe and promising strategy for the clinical treatment of infected wounds.
基金supported by NUS Presidential Young Professorship,MOE Tier 1 grantsupported by the NUS Research Scholarship.
文摘Bone marrow-derived mesenchymal stem cell(MSC)is one of the most actively studied cell types due to its regenerative potential and immunomodulatory properties.Conventional cell expansion methods using 2D tissue culture plates and 2.5D microcarriers in bioreactors can generate large cell numbers,but they compromise stem cell potency and lack mechanical preconditioning to prepare MSC for physiological loading expected in vivo.To overcome these challenges,in this work,we describe a 3D dynamic hydrogel using magneto-stimulation for direct MSC manufacturing to therapy.With our technology,we found that dynamic mechanical stimulation(DMS)enhanced matrix-integrinβ1 interactions which induced MSCs spreading and proliferation.In addition,DMS could modulate MSC biofunctions including directing MSC differentiation into specific lineages and boosting paracrine activities(e.g.,growth factor secretion)through YAP nuclear localization and FAK-ERK pathway.With our magnetic hydrogel,complex procedures from MSC manufacturing to final clinical use,can be integrated into one single platform,and we believe this‘all-in-one’technology could offer a paradigm shift to existing standards in MSC therapy.
基金funding support from the NUS Presidential Young Professorship and NUS Technological Innovations in Infectious Diseases Translational Research.
文摘Microbial resistance to current antibiotics therapies is a major cause of implant failure and adverse clinical outcomes in orthopaedic surgery.Recent developments in advanced antimicrobial nanotechnologies provide numerous opportunities to effective remove resistant bacteria and prevent resistance from occurring through unique mechanisms.With tunable physicochemical properties,nanomaterials can be designed to be bactericidal,antifouling,immunomodulating,and capable of delivering antibacterial compounds to the infection region with spatiotemporal accuracy.Despite its substantial advancement,an important,but under-explored area,is potential microbial resistance to nanomaterials and how this can impact the clinical use of antimicrobial nanotechnologies.This review aims to provide a better understanding of nanomaterial-associated microbial resistance to accelerate bench-to-bedside translations of emerging nanotechnologies for effective control of implant associated infections.
文摘The impact of the composition of blends comprising low‐density polyethylene(LDPE),isotactic polypropylene(PP)and a styrenic copolymer additive on the thermomechanical properties as well as the direct‐current(DC)electrical and thermal conductivity is investigated.The presence of 5 weight percent(wt%)of the styrenic copolymer strongly reduces the amount of PP that is needed to enhance the storage modulus above the melting temperature of LDPE from 40 to 24 wt%.At the same time,the copolymer improves the consistency of the thermomechanical properties of the resulting ternary blends.While both the DC electrical and thermal conductivity strongly decrease with PP content,the addition of the styrenic copolymer appears to have little influence on either property.Evidently,PP in combination with small amounts of a styrenic copolymer not only allows to reinforce LDPE at elevated temperatures but also functions as an electrical conductivity‐reducing additive,which makes such thermoplastic ternary formulations possible candidates for the insulation of high‐voltage power cables.
基金National Science Foundation for Excellent Young Project,Grant/Award Number:82322042National Key Research and Development Program of China,Grant/Award Number:2021YFC2302200+2 种基金National Natural Science Foundation of China,Grant/Award Number:82272248Training Project of National Science Foundation for Outstanding/Distinguished Young Scholars of Southern Medical University,Grant/Award Number:C620PF0217Science Fund for Distinguished Young Scholars of Guangdong Province,Grant/Award Number:2022B1515020089。
文摘Infectious diseases present significant challenges to global health,thereby extensively affecting both human society and the economy.In recent years,functional probes have demonstrated remarkable potential as crucial biomedical media for the research and treatment of infectious diseases.Their applications in the realm of infectious diseases include pathogen detection,exploration of biological mechanisms,and development of anti-infective drugs.This review provides a concise introduction to the severity,classification,and pathogenesis of infectious diseases.Subsequently,we examined the distinctiveness and design strategies of functional probes for diagnosing and treating infectious diseases,shedding light on their design rationale using typical examples.We discuss the current status and challenges associated with the clinical implementation of functional probes.Furthermore,we explored the prospects of using these probes for the diagnosis and treatment of infectious diseases.This review aims to offer novel insights into the design of diagnostic probes for infectious diseases and broaden their applications in disease treatment.
文摘Single-cell encapsulation in droplet microfluidics is commonly hindered by the tradeoff between cell suspension density and on-chip focusing performance.In this study,we introduce a novel droplet microfluidic chip to overcome this challenge.The chip comprises a double spiral focusing unit,a flow resistance-based sample enrichment module with fine-tunable outlets,and a crossflow droplet generation unit.Utilizing a low-density cell/bead suspension(2×10^(6) objects/mL),cells/beads are focused into a near-equidistant linear arrangement within the double spiral microchannel.The excess water phase is diverted while cells/beads remain focused and sequentially encapsulated in individual droplets.Focusing performance was assessed through numerical simulations and experiments at three flow rates(40,60,80μL/min),demonstrating successful focusing at 40 and 80μL/min for beads and cells,respectively.In addition,both simulation and experimental results revealed that the flow resistance at the sample enrichment module is adjustable by punching different outlets,allowing over 50%of the aqueous phase to be removed.YOLOv8n-based droplet detection algorithms realized the counting of cells/beads in droplets,statistically demonstrating single-cell and bead encapsulation rates of 72.2%and 79.2%,respectively.All the results indicate that this on-chip sample enrichment approach can be further developed and employed as a critical component in single-cell encapsulation in water-in-oil droplets.
基金funded by the USDA Agricultural Marketing Service,Specialty Crop Block Grant Program,as USDA-AMS award#AM190100XXXXG055.
文摘Microgreens are young,tender greens that are used to enhance the color,texture,or flavor of salads and main dishes.They can be grown in small scales and indoors,making them widely adopted by controlled environment agriculture,an indoor farming practice is particularly important for feeding increasing urban populations.Besides,microgreens are attracting more consumers’attention due to their high nutritional value and unique sensory characteristics.This review focuses on the nutrition quality,sensory evaluation,pre-and post-harvest interventions,and health benefits of microgreens.Microgreens are rich in vitamins(e.g.,VC),minerals(e.g.,copper and zinc),and phytochemicals,including carotenoids and phenolic compounds,which act as antioxidants in human body.Pre-harvest interventions,such as illumination,salinity stress,nutrient fortification,and natural substrates,infl uence the photosynthetic and metabolic activities of microgreens and were shown to improve their nutritional quality,while the effects varied among species.After harvesting,packaging method and storage temperature can infl uence the nutrient retention in microgreens.Both in vitro and in vivo studies have shown that microgreens have anti-infl ammatory,anti-cancer,anti-bacterial,and anti-hyperglycemia properties,making it a new functional food beneficial to human health.The sensory attributes and overall acceptability and liking of microgreens are primarily infl uenced by their phytochemical content.Microgreens are only getting popular during the last decades and research on microgreens is still at its early stage.More studies are warranted to optimize the pre-and post-harvest practices for nutrient enhancement and retention and to explore the potential health benefits of different microgreens for the prevention and treatment of chronic diseases.
