BACKGROUND: The duration of viremia during hepatitis E virus (HEV) infection has rarely been reported. This study was undertaken to detect HEV RNA in sera of patients with hepatitis E and to understand the process of ...BACKGROUND: The duration of viremia during hepatitis E virus (HEV) infection has rarely been reported. This study was undertaken to detect HEV RNA in sera of patients with hepatitis E and to understand the process of HEV infection more thoroughly. METHODS: HEV RNA was detected in the serum samples of hospitalized patients with acute hepatitis E by reverse transcriptase-nested polymerase chain reaction (RT-nPCR) using two pairs of primers from open reading frame (ORF) I of the HEV genome. RESULTS: The serum samples from 44 (70%) of 62 patients were positive for HEV RNA. Thirty-two of these patients, with 288 serial serum specimens, were followed up for the whole process, and 24 patients (75%) were positive for HEV RNA. The positive rates declined with the course of the disease, serum HEV RNA persisting for 20.6 days on average after onset of illness. Serum HEV RNA remained positive in 36 (81.8%) of the 44 patients at the time their alanine aminotransferase (ALT) began to decrease. There was no difference in HEV RNA positivity between serum with high levels of HEV antibody (peak P/N ratio >= 4.0) and that with low levels (peak P/N ratio 4.0), with 25 out of 35 and 19 out of 27 (71.4% vs. 70.4%, P>0.05), respectively. CONCLUSIONS: There is a relatively long period of HEV viremia in patients with hepatitis E. The proportion of HEV viremia and its duration are not directly related to serum ALT values or HEV antibody levels.展开更多
Objective To analyze the pathogenic distribution characteristics of elderly inpatients with urinary tract infection(UTI).Methods Retrospective analysis of the clinical data and pathogen detection of 809 patients with ...Objective To analyze the pathogenic distribution characteristics of elderly inpatients with urinary tract infection(UTI).Methods Retrospective analysis of the clinical data and pathogen detection of 809 patients with UTI aged≥60 years admitted to The First Affiliated Hospital of China Medical University from January 2019 to January 2023 was performed.The differences in the pathogen distribution of patients with UTI were compared between groups of 60 to 79 years old and≥80 years old.The chi-square test was used for statistical analysis.Multivariate logistic regression analysis was used to analyze the risk factors for bacterial production of extended spectrumβlactamase(ESBL).Results A total of 914 strains of pathogens were isolated from 809 patients,including 584 strains isolated from 60 to 79 years old patients,330 strains isolated from≥80 years old patients,and 62 patients had mixed infections with bacteria and fungi.In the group of patients≥80 years old,there were 144(43.64%)Gram-negative bacteria.Escherichia coli(16.67%(55/330)).展开更多
Objective To investigate the risk factors for kidney injury during anti-retroviral therapy (ART) with zidovudine(AZT) or tenofovir disoproxil fumarate(TDF) in human immunodeficiency virus/acquired immunodeficiency syn...Objective To investigate the risk factors for kidney injury during anti-retroviral therapy (ART) with zidovudine(AZT) or tenofovir disoproxil fumarate(TDF) in human immunodeficiency virus/acquired immunodeficiency syndrome(HIV/AIDS) patients,and to construct and validate a prediction model for the risk of kidney injury in HIV/AIDS patients based on a nomogram.展开更多
文摘BACKGROUND: The duration of viremia during hepatitis E virus (HEV) infection has rarely been reported. This study was undertaken to detect HEV RNA in sera of patients with hepatitis E and to understand the process of HEV infection more thoroughly. METHODS: HEV RNA was detected in the serum samples of hospitalized patients with acute hepatitis E by reverse transcriptase-nested polymerase chain reaction (RT-nPCR) using two pairs of primers from open reading frame (ORF) I of the HEV genome. RESULTS: The serum samples from 44 (70%) of 62 patients were positive for HEV RNA. Thirty-two of these patients, with 288 serial serum specimens, were followed up for the whole process, and 24 patients (75%) were positive for HEV RNA. The positive rates declined with the course of the disease, serum HEV RNA persisting for 20.6 days on average after onset of illness. Serum HEV RNA remained positive in 36 (81.8%) of the 44 patients at the time their alanine aminotransferase (ALT) began to decrease. There was no difference in HEV RNA positivity between serum with high levels of HEV antibody (peak P/N ratio >= 4.0) and that with low levels (peak P/N ratio 4.0), with 25 out of 35 and 19 out of 27 (71.4% vs. 70.4%, P>0.05), respectively. CONCLUSIONS: There is a relatively long period of HEV viremia in patients with hepatitis E. The proportion of HEV viremia and its duration are not directly related to serum ALT values or HEV antibody levels.
文摘Objective To analyze the pathogenic distribution characteristics of elderly inpatients with urinary tract infection(UTI).Methods Retrospective analysis of the clinical data and pathogen detection of 809 patients with UTI aged≥60 years admitted to The First Affiliated Hospital of China Medical University from January 2019 to January 2023 was performed.The differences in the pathogen distribution of patients with UTI were compared between groups of 60 to 79 years old and≥80 years old.The chi-square test was used for statistical analysis.Multivariate logistic regression analysis was used to analyze the risk factors for bacterial production of extended spectrumβlactamase(ESBL).Results A total of 914 strains of pathogens were isolated from 809 patients,including 584 strains isolated from 60 to 79 years old patients,330 strains isolated from≥80 years old patients,and 62 patients had mixed infections with bacteria and fungi.In the group of patients≥80 years old,there were 144(43.64%)Gram-negative bacteria.Escherichia coli(16.67%(55/330)).
文摘Objective To investigate the risk factors for kidney injury during anti-retroviral therapy (ART) with zidovudine(AZT) or tenofovir disoproxil fumarate(TDF) in human immunodeficiency virus/acquired immunodeficiency syndrome(HIV/AIDS) patients,and to construct and validate a prediction model for the risk of kidney injury in HIV/AIDS patients based on a nomogram.
