探讨微滴数字PCR(Droplet digital PCR,ddPCR)技术在猪肉掺假检测中的应用价值。提取猪肉肌肉组织中的基因组DNA,设计猪源性内标基因引物及探针序列,以ddPCR进行扩增读取结果,在不同物种中进行特异性检测,利用标准质粒DNA检测最低下限...探讨微滴数字PCR(Droplet digital PCR,ddPCR)技术在猪肉掺假检测中的应用价值。提取猪肉肌肉组织中的基因组DNA,设计猪源性内标基因引物及探针序列,以ddPCR进行扩增读取结果,在不同物种中进行特异性检测,利用标准质粒DNA检测最低下限拷贝浓度;在不同掺杂量组织中检测最低比例。结果表明,猪源性ddPCR检测体系特异强,在10~2000 copies/μL的范围内具有较高的检测线性,RSD值小于25%,可检测至猪肉掺杂量为1%的混合样品。基于ddPCR的检测猪肉掺假的方法具有较高的实际应用价值。展开更多
A simple and rapid optical biosensor for the determination of ammonium was developed by immobilization of gluta-mate dehydrogenase (GLDH) and diaphorase (Dph) in chitosan film coated on a glass slide employing thiazol...A simple and rapid optical biosensor for the determination of ammonium was developed by immobilization of gluta-mate dehydrogenase (GLDH) and diaphorase (Dph) in chitosan film coated on a glass slide employing thiazolyl blue tetrazolium bromide (MTT) as a color indicator. The developed biosensor displays a purple color formation of formazan attributed to the unreacted NADH in the reaction system in the presence of ammonium. The color intensity was found to decrease proportionally with the increase of ammonium concentrations after 10 min exposure. The linearity of the biosensor towards ammonium was in the range of 16.8 – 70 μM (R2 = 0.9955) with detection limit of 11 μM. A good agreement (R2 = 0.9984) with indothymol method was obtained in the measurement of fish pond water samples. The effect of potential interferences such as metals ion has also been evaluated.展开更多
A spectrophotometer with an LED as the light source for uric acid detection is proposed in this work. The mechanism of uric acid detection is based on energy absorbed by sodium urate, which is a chemical product of ur...A spectrophotometer with an LED as the light source for uric acid detection is proposed in this work. The mechanism of uric acid detection is based on energy absorbed by sodium urate, which is a chemical product of uric acid and sodium hydroxide solution. For the performance validation, comparison between the spectrophotometer with an LED and halogen lamp is carried out. Measurement results suggest that the spectrophotometer system with LED light has better sensitivity than that with halogen light. At a 460 nm wavelength,the sensitivity for the spectrophotometer with an LED is 0.0046 dL/mg, which is 73% higher than that with halogen light that records 0.0012 dL/mg. This enhanced sensitivity is attributed to the higher luminous efficacy of the LED light beam. As a result, a larger amount of flux interacts with the sample, leading to the sensitivity enhancement. The spectrophotometer with an LED is also applied for the detection of uric acid in a real human urine sample. Based on the experimental data at a 460 nm wavelength, the method manages to achieve the sensitivity of 0.0016 dL/mg, accuracy of 96.01%, limit of detection of 4.79 mg/dL, and limit of quantification of 14.52 mg/dL. These findings show that the use of LED as the input light source is promising for the spectrophotometer.展开更多
文摘探讨微滴数字PCR(Droplet digital PCR,ddPCR)技术在猪肉掺假检测中的应用价值。提取猪肉肌肉组织中的基因组DNA,设计猪源性内标基因引物及探针序列,以ddPCR进行扩增读取结果,在不同物种中进行特异性检测,利用标准质粒DNA检测最低下限拷贝浓度;在不同掺杂量组织中检测最低比例。结果表明,猪源性ddPCR检测体系特异强,在10~2000 copies/μL的范围内具有较高的检测线性,RSD值小于25%,可检测至猪肉掺杂量为1%的混合样品。基于ddPCR的检测猪肉掺假的方法具有较高的实际应用价值。
文摘A simple and rapid optical biosensor for the determination of ammonium was developed by immobilization of gluta-mate dehydrogenase (GLDH) and diaphorase (Dph) in chitosan film coated on a glass slide employing thiazolyl blue tetrazolium bromide (MTT) as a color indicator. The developed biosensor displays a purple color formation of formazan attributed to the unreacted NADH in the reaction system in the presence of ammonium. The color intensity was found to decrease proportionally with the increase of ammonium concentrations after 10 min exposure. The linearity of the biosensor towards ammonium was in the range of 16.8 – 70 μM (R2 = 0.9955) with detection limit of 11 μM. A good agreement (R2 = 0.9984) with indothymol method was obtained in the measurement of fish pond water samples. The effect of potential interferences such as metals ion has also been evaluated.
基金Universiti Tun Hussein Onn Malaysia (UTHM) for supporting this research work under the Tier 1 University Grant Scheme (No. H162)
文摘A spectrophotometer with an LED as the light source for uric acid detection is proposed in this work. The mechanism of uric acid detection is based on energy absorbed by sodium urate, which is a chemical product of uric acid and sodium hydroxide solution. For the performance validation, comparison between the spectrophotometer with an LED and halogen lamp is carried out. Measurement results suggest that the spectrophotometer system with LED light has better sensitivity than that with halogen light. At a 460 nm wavelength,the sensitivity for the spectrophotometer with an LED is 0.0046 dL/mg, which is 73% higher than that with halogen light that records 0.0012 dL/mg. This enhanced sensitivity is attributed to the higher luminous efficacy of the LED light beam. As a result, a larger amount of flux interacts with the sample, leading to the sensitivity enhancement. The spectrophotometer with an LED is also applied for the detection of uric acid in a real human urine sample. Based on the experimental data at a 460 nm wavelength, the method manages to achieve the sensitivity of 0.0016 dL/mg, accuracy of 96.01%, limit of detection of 4.79 mg/dL, and limit of quantification of 14.52 mg/dL. These findings show that the use of LED as the input light source is promising for the spectrophotometer.
