This work examines the use of communication channels to sensitize the people about life threatening diseases with special emphasis on Hepatitis. It becomes very topical at this moment because most of the viral infecti...This work examines the use of communication channels to sensitize the people about life threatening diseases with special emphasis on Hepatitis. It becomes very topical at this moment because most of the viral infections wreak havoc such as Acquired Immune Deficiency Syndrome (AIDS) and more recently, Ebola Virus Disease (EVD) assumed an epidemic proportion because the people were not adequately sensitized with precautionary measures against them. One of such measures is dissemination of useful information on how to avoid or contain such viral infections. It also concerns itself with hepatitis because of its contagious nature and the way it attacks the liver. The study reviews relevant concepts to information dissemination. It discovers that as deadly as the disease is, most people, including some health attendants in hospitals do not understand what it means and how it is contracted. In addition, it was discovered that 60% of the total respondents did not know about hepatitis. 73% of those who claimed they knew about it knew through friends and not through the media. 80% of those who claimed they sought health information from the media did not know about hepatitis. 65% of those who claimed they had high access to radio did not really seek health information from the radio. In the communities in Nigeria, we suggest that there should be increased public awareness about viral diseases, complete immunization against viral hepatitis and better sanitation facilities. Other efforts by the Government should include the provision of safe drinking water;expansion of well-equipped hospitals among other health developmental programmes will go a long way in the reduction of hepatitis in communities, among pregnant women as well as its associated maternal effects in the communities.展开更多
Yoghurt is fermented milk which results from the exclusive action of <i><span style="font-family:Verdana;">Lactobacillus</span></i> <i><span style="font-family:Verdana;&...Yoghurt is fermented milk which results from the exclusive action of <i><span style="font-family:Verdana;">Lactobacillus</span></i> <i><span style="font-family:Verdana;">delbrueckii</span></i><span style="font-family:Verdana;"> subsp </span><i><span style="font-family:Verdana;">bulgaricus</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Streptococcus</span></i> <i><span style="font-family:Verdana;">thermophilus</span></i><span style="font-family:Verdana;">. However, a disruption of the bacterial growth can lead to significant industrial losses. Currents monitoring methods, based on the checking of the pH during the fermentation step are not always satisfactory. The NeOse Pro system, a portable electronic nose, is a mean to obtain immediately aromatic profiles. In this work, we applied this technology to the yoghurt ecosystem, a highly hydrated product. The profiles obtained allowed us to discriminate yoghurts before and after fermentation. In detail, the discrepancy between the two bacteria cultured alone was slight. It was also the case when we compared different initial bacterial ratios. However, two different mixes of bacteria led to clearly distinguishable profiles. A GC/MS analysis performed on products fermented 7</span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span>h allowed us to explain the detection of acetaldehyde by the stimulation of some captors of the apparatus. NeOse Pro is so convenient to study the fermentation of yogurt.</span></span></span>展开更多
Crop residues are among the main inputs that allow the organic carbon(C)and nutrients to be maintained in agricultural soil.It is an important management strategy that can improve soil fertility and enhance agricultur...Crop residues are among the main inputs that allow the organic carbon(C)and nutrients to be maintained in agricultural soil.It is an important management strategy that can improve soil fertility and enhance agricultural productivity.This work aims to evaluate the extent of the changes that may occur in the soil heterotrophic microbial communities involved in organic matter decomposition and C and nitrogen(N)mineralization after the addition of crop residues.Soil microcosm experiments were performed at 28℃ for 90 days with the addition of three crop residues with contrasting biochemical qualities:pea(Pisum sativum L.),rapeseed(Brassica napus L.),and wheat(Triticum aestivum L.).Enzyme activities,C and N mineralization,and bacterial and fungal biomasses were monitored,along with the bacterial and fungal community composition,by the highthroughput sequencing of 16 S rRNA and ITS genes.The addition of crop residues caused decreases in β-glucosidase and arylamidase activities and simultaneous enhancement of the C mineralization and net N immobilization,which were linked to changes in the soil microbial communities.The addition of crop residues decreased the bacterial and fungal biomasses 90 days after treatment and there were shifts in bacterial and fungal diversity at the phyla,order,and genera levels.Some specific orders and genera were dependent on crop residue type.For example,Chloroflexales,Inquilinus,Rubricoccus,Clitocybe,and Verticillium were identified in soils with pea residues;whereas Thermoanaerobacterales,Thermacetogenum,and Hypoxylon were enriched in soils with rapeseed residues,and Halanaerobiales,Rubrobacter,and Volutella were only present in soils with wheat residues.The findings of this study suggest that soil C and N dynamics in the presence of the crop residues were driven by the selection of specific bacterial and fungal decomposers linked to the biochemical qualities of the crop residues.If crop residue decomposition processes showed specific bacterial and fungal operational taxonomic unit(OTU)signatures,this study also suggests a strong functional redundancy that exists among soil microbial communities.展开更多
Heat treatment of milk may cause whey proteins and caseins to form aggregates. These soluble and micellar aggregates and their other properties (size, composition, shape, etc.) can affect the techno-functionalities to...Heat treatment of milk may cause whey proteins and caseins to form aggregates. These soluble and micellar aggregates and their other properties (size, composition, shape, etc.) can affect the techno-functionalities to the milk, conferring interesting or negative features depending on the application in dairy industries. In this study, we propose a new approach to characterise those protein aggregates. SDS-agarose electrophoresis is followed by the calculation of a retention factor (Rf) for each protein spot. Rf allows milk aggregates to be compared qualitatively under the same conditions. This method could be transposed to the dairy industry for a better knowledge of the milk subsequent to heat treatment.展开更多
Multifunctional and diversified agriculture can address diverging pressures and demands by simultaneously enhancing productivity,biodiversity,and the provision of ecosystem services.The use of digital technologies can...Multifunctional and diversified agriculture can address diverging pressures and demands by simultaneously enhancing productivity,biodiversity,and the provision of ecosystem services.The use of digital technologies can support this by designing and managing resource-efficient and context-specific agricultural systems.We present the Digital Agricultural Knowledge and Information System(DAKIS)to demonstrate an approach that employs digital technologies to enable decision-making towards diversified and sustainable agriculture.To develop the DAKIS,we specified,together with stakeholders,requirements for a knowledge-based decision-support tool and reviewed the literature to identify limitations in the current generation of tools.The results of the review point towards recurring challenges regarding the consideration of ecosystem services and biodiversity,the capacity to foster communication and cooperation between farmers and other actors,and the ability to link multiple spatiotemporal scales and sustainability levels.To overcome these challenges,the DAKIS provides a digital platform to support farmers'decision-making on land use and management via an integrative spatiotemporally explicit approach that analyses a wide range of data from various sources.The approach integrates remote and in situ sensors,artificial intelligence,modelling,stakeholder-stated demand for biodiversity and ecosystem services,and participatory sustainability impact assessment to address the diverse drivers affecting agricultural land use and management design,including natural and agronomic factors,economic and policy considerations,and socio-cultural preferences and settings.Ultimately,the DAKIS embeds the consideration of ecosystem services,biodiversity,and sustainability into farmers'decision-making and enables learning and progress towards site-adapted small-scale multifunctional and diversified agriculture while simultaneously supporting farmers'objectives and societal demands.展开更多
The effect of enzymatic crosslinking by microbial transglutaminase on the physico-chemical properties of gelatin-based films incorporated with lysozyme were investigated.Increase in the crosslinking degree of gelatin ...The effect of enzymatic crosslinking by microbial transglutaminase on the physico-chemical properties of gelatin-based films incorporated with lysozyme were investigated.Increase in the crosslinking degree of gelatin with microbial transglutaminase concentration in film-forming suspensions was correlated with a decrease of films thickness.Moreover,the enhancement in crosslinking resulted in a decrease of the solubility in water,as well as of the extent of swelling degree,and of the water vapor permeability of films and in an increase of films tensile strength.The kinetics of release at 4◦C of lysozyme from control non-reticulated films and reticulated films to either sodium acetate buffer,or 2%(w/v)agar gels in direct contact were compared.Interestingly,the kinetics of release of lysozyme were always slower from crosslinked films than from control films.This difference was higher for lysozyme release to agar gels,which was dominated by Fickian diffusion.Enzymatic crosslinking by microbial transglutaminase of gelatin-based films incorporated with lysozyme can thus effectively control the release of this food preservative.展开更多
In this study,the interaction of exopolysaccharides from Leuconostoc mesenteroides P35(EPS-LM)with Escherichia coli heat-labile enterotoxin B-pentamer(LTB)was investigated at different concentrations and temperatures ...In this study,the interaction of exopolysaccharides from Leuconostoc mesenteroides P35(EPS-LM)with Escherichia coli heat-labile enterotoxin B-pentamer(LTB)was investigated at different concentrations and temperatures by using surface plasmon resonance(SPR)and molecular docking approaches.FT-IR spectral analysis together with HPTLC analysis revealing that glucose is the only constitutive monosaccharide of EPS-LM suggests that its structure is composed of dextran withα-D(1→6)glycosidic linkages.SPR analysis revealed the high affinity of EPS-LM for immobilized LTB toxin(KA=(2.05±0.04)×10^(6) mol.L−1 at 37℃).The binding process was spontaneous(ΔG<0),endothermic(ΔH>0),and entropy-driven(ΔS>0)with an increase of KA with temperature.This suggests that EPS-LM-LTB interaction is dominated by hydrophobic forces.The binding affinity of EPS-LM to LTB had negligible dependence on enthalpy(ΔH=0.084 kJ mol−1).Further,molecular docking results suggested the presence of some binding sites of EPS-LM on the LTB through hydrophobic forces(Lys,Asp,Arg,Glu)and also hydrogen bonding(Glu)in the hydrophobic core of LTB.Besides autodock studies,Schiffer-Edmundson helical wheel diagrams of LTB inα-helix domain suggested that LTB hydrophobic core is a highly effective region,which was able to form favorable non-polar interactions of the protein's binding surface(with amino acids residues such as Tyr,Leu,Ile)with EPS-LM.This study provided thus further insights into the interactions between EPS-LM and LTB,suggesting that EPS produced by some LAB,such as EPS produced by Ln.mesenteroides P35 strain are good candidates to inhibit E.coli toxin activity.展开更多
Background Kernza®intermediate wheatgrass is a perennial grain and forage crop that can provide several ecosystem services.Major research efforts focused on Kernza have taken place in high latitudes.The goal of t...Background Kernza®intermediate wheatgrass is a perennial grain and forage crop that can provide several ecosystem services.Major research efforts focused on Kernza have taken place in high latitudes.The goal of this study was to evaluate,for the first time,the agronomic performance of Kernza in a low-latitude region with mild winters.Methods A KS-cycle 4 Kernza population(A)was planted in spring in Wisconsin,USA,and selected in one cycle for lower vernalization requirements,obtaining a new population(B).These two populations,at three nitrogen(N)fertilization rates,were evaluated in a full factorial,completely randomized field experiment in Uruguay over 2 years.Results The populations were similar in grain yields and flowering time in the 1st year,but population B had 63%lower grain yield in the 2nd year and 20%lower forage yield throughout the experiment.Increasing the N rate to 160 kg ha−1 led to a 63%increase in grain yield and 28%increase in forage yield across populations.Forage yields and nutritive values were similar to those reported in the northern hemisphere.However,grain yields for both the 1st(316 kg ha−1)and 2nd year(41 kg ha−1)were lower due to reduced flowering and weed competition.Conclusions Expansion of Kernza to lower-latitude regions will require further breeding to improve reproductive performance.展开更多
文摘This work examines the use of communication channels to sensitize the people about life threatening diseases with special emphasis on Hepatitis. It becomes very topical at this moment because most of the viral infections wreak havoc such as Acquired Immune Deficiency Syndrome (AIDS) and more recently, Ebola Virus Disease (EVD) assumed an epidemic proportion because the people were not adequately sensitized with precautionary measures against them. One of such measures is dissemination of useful information on how to avoid or contain such viral infections. It also concerns itself with hepatitis because of its contagious nature and the way it attacks the liver. The study reviews relevant concepts to information dissemination. It discovers that as deadly as the disease is, most people, including some health attendants in hospitals do not understand what it means and how it is contracted. In addition, it was discovered that 60% of the total respondents did not know about hepatitis. 73% of those who claimed they knew about it knew through friends and not through the media. 80% of those who claimed they sought health information from the media did not know about hepatitis. 65% of those who claimed they had high access to radio did not really seek health information from the radio. In the communities in Nigeria, we suggest that there should be increased public awareness about viral diseases, complete immunization against viral hepatitis and better sanitation facilities. Other efforts by the Government should include the provision of safe drinking water;expansion of well-equipped hospitals among other health developmental programmes will go a long way in the reduction of hepatitis in communities, among pregnant women as well as its associated maternal effects in the communities.
文摘Yoghurt is fermented milk which results from the exclusive action of <i><span style="font-family:Verdana;">Lactobacillus</span></i> <i><span style="font-family:Verdana;">delbrueckii</span></i><span style="font-family:Verdana;"> subsp </span><i><span style="font-family:Verdana;">bulgaricus</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">Streptococcus</span></i> <i><span style="font-family:Verdana;">thermophilus</span></i><span style="font-family:Verdana;">. However, a disruption of the bacterial growth can lead to significant industrial losses. Currents monitoring methods, based on the checking of the pH during the fermentation step are not always satisfactory. The NeOse Pro system, a portable electronic nose, is a mean to obtain immediately aromatic profiles. In this work, we applied this technology to the yoghurt ecosystem, a highly hydrated product. The profiles obtained allowed us to discriminate yoghurts before and after fermentation. In detail, the discrepancy between the two bacteria cultured alone was slight. It was also the case when we compared different initial bacterial ratios. However, two different mixes of bacteria led to clearly distinguishable profiles. A GC/MS analysis performed on products fermented 7</span><span><span><span style="font-family:;" "=""> </span></span></span><span><span><span>h allowed us to explain the detection of acetaldehyde by the stimulation of some captors of the apparatus. NeOse Pro is so convenient to study the fermentation of yogurt.</span></span></span>
基金funded by the Normandy region and was supported by the Vivepois Project(D16-12746),France。
文摘Crop residues are among the main inputs that allow the organic carbon(C)and nutrients to be maintained in agricultural soil.It is an important management strategy that can improve soil fertility and enhance agricultural productivity.This work aims to evaluate the extent of the changes that may occur in the soil heterotrophic microbial communities involved in organic matter decomposition and C and nitrogen(N)mineralization after the addition of crop residues.Soil microcosm experiments were performed at 28℃ for 90 days with the addition of three crop residues with contrasting biochemical qualities:pea(Pisum sativum L.),rapeseed(Brassica napus L.),and wheat(Triticum aestivum L.).Enzyme activities,C and N mineralization,and bacterial and fungal biomasses were monitored,along with the bacterial and fungal community composition,by the highthroughput sequencing of 16 S rRNA and ITS genes.The addition of crop residues caused decreases in β-glucosidase and arylamidase activities and simultaneous enhancement of the C mineralization and net N immobilization,which were linked to changes in the soil microbial communities.The addition of crop residues decreased the bacterial and fungal biomasses 90 days after treatment and there were shifts in bacterial and fungal diversity at the phyla,order,and genera levels.Some specific orders and genera were dependent on crop residue type.For example,Chloroflexales,Inquilinus,Rubricoccus,Clitocybe,and Verticillium were identified in soils with pea residues;whereas Thermoanaerobacterales,Thermacetogenum,and Hypoxylon were enriched in soils with rapeseed residues,and Halanaerobiales,Rubrobacter,and Volutella were only present in soils with wheat residues.The findings of this study suggest that soil C and N dynamics in the presence of the crop residues were driven by the selection of specific bacterial and fungal decomposers linked to the biochemical qualities of the crop residues.If crop residue decomposition processes showed specific bacterial and fungal operational taxonomic unit(OTU)signatures,this study also suggests a strong functional redundancy that exists among soil microbial communities.
文摘Heat treatment of milk may cause whey proteins and caseins to form aggregates. These soluble and micellar aggregates and their other properties (size, composition, shape, etc.) can affect the techno-functionalities to the milk, conferring interesting or negative features depending on the application in dairy industries. In this study, we propose a new approach to characterise those protein aggregates. SDS-agarose electrophoresis is followed by the calculation of a retention factor (Rf) for each protein spot. Rf allows milk aggregates to be compared qualitatively under the same conditions. This method could be transposed to the dairy industry for a better knowledge of the milk subsequent to heat treatment.
基金This work was made possible through funding from the Digital Agriculture Knowledge and Information System(DAKIS)Project(ID:FKZ 031B0729A)financed by the German Federal Ministry of Education and Research(BMBF).Sincere thanks to Amir Armaghan for his amazing sketches on the DAKIS GUI,enabling us to approach the work from the user's perspective.We acknowledge the valuable contributions of Stefan Zachaeus,Sebastian Möller and Nils Niemann on the design of the DAKIS back end.We thank the many other members of the DAKIS crew that one way or another contribute expertise and input to the development of the DAKIS.
文摘Multifunctional and diversified agriculture can address diverging pressures and demands by simultaneously enhancing productivity,biodiversity,and the provision of ecosystem services.The use of digital technologies can support this by designing and managing resource-efficient and context-specific agricultural systems.We present the Digital Agricultural Knowledge and Information System(DAKIS)to demonstrate an approach that employs digital technologies to enable decision-making towards diversified and sustainable agriculture.To develop the DAKIS,we specified,together with stakeholders,requirements for a knowledge-based decision-support tool and reviewed the literature to identify limitations in the current generation of tools.The results of the review point towards recurring challenges regarding the consideration of ecosystem services and biodiversity,the capacity to foster communication and cooperation between farmers and other actors,and the ability to link multiple spatiotemporal scales and sustainability levels.To overcome these challenges,the DAKIS provides a digital platform to support farmers'decision-making on land use and management via an integrative spatiotemporally explicit approach that analyses a wide range of data from various sources.The approach integrates remote and in situ sensors,artificial intelligence,modelling,stakeholder-stated demand for biodiversity and ecosystem services,and participatory sustainability impact assessment to address the diverse drivers affecting agricultural land use and management design,including natural and agronomic factors,economic and policy considerations,and socio-cultural preferences and settings.Ultimately,the DAKIS embeds the consideration of ecosystem services,biodiversity,and sustainability into farmers'decision-making and enables learning and progress towards site-adapted small-scale multifunctional and diversified agriculture while simultaneously supporting farmers'objectives and societal demands.
文摘The effect of enzymatic crosslinking by microbial transglutaminase on the physico-chemical properties of gelatin-based films incorporated with lysozyme were investigated.Increase in the crosslinking degree of gelatin with microbial transglutaminase concentration in film-forming suspensions was correlated with a decrease of films thickness.Moreover,the enhancement in crosslinking resulted in a decrease of the solubility in water,as well as of the extent of swelling degree,and of the water vapor permeability of films and in an increase of films tensile strength.The kinetics of release at 4◦C of lysozyme from control non-reticulated films and reticulated films to either sodium acetate buffer,or 2%(w/v)agar gels in direct contact were compared.Interestingly,the kinetics of release of lysozyme were always slower from crosslinked films than from control films.This difference was higher for lysozyme release to agar gels,which was dominated by Fickian diffusion.Enzymatic crosslinking by microbial transglutaminase of gelatin-based films incorporated with lysozyme can thus effectively control the release of this food preservative.
基金the Minist`ere des Affaires Etrang`eres(France)and the Ministry of Research,Science and Technology(Iran)for financing double PhD scholarship joint-supervision program between France and Iran for Mojtaba AZARI-ANPARThe authors are indebted to Conseil D´epartemental de l’Ain and Bourg en Bresse Agglom´eration for the financial support of BioDyMIA research unit activities.
文摘In this study,the interaction of exopolysaccharides from Leuconostoc mesenteroides P35(EPS-LM)with Escherichia coli heat-labile enterotoxin B-pentamer(LTB)was investigated at different concentrations and temperatures by using surface plasmon resonance(SPR)and molecular docking approaches.FT-IR spectral analysis together with HPTLC analysis revealing that glucose is the only constitutive monosaccharide of EPS-LM suggests that its structure is composed of dextran withα-D(1→6)glycosidic linkages.SPR analysis revealed the high affinity of EPS-LM for immobilized LTB toxin(KA=(2.05±0.04)×10^(6) mol.L−1 at 37℃).The binding process was spontaneous(ΔG<0),endothermic(ΔH>0),and entropy-driven(ΔS>0)with an increase of KA with temperature.This suggests that EPS-LM-LTB interaction is dominated by hydrophobic forces.The binding affinity of EPS-LM to LTB had negligible dependence on enthalpy(ΔH=0.084 kJ mol−1).Further,molecular docking results suggested the presence of some binding sites of EPS-LM on the LTB through hydrophobic forces(Lys,Asp,Arg,Glu)and also hydrogen bonding(Glu)in the hydrophobic core of LTB.Besides autodock studies,Schiffer-Edmundson helical wheel diagrams of LTB inα-helix domain suggested that LTB hydrophobic core is a highly effective region,which was able to form favorable non-polar interactions of the protein's binding surface(with amino acids residues such as Tyr,Leu,Ile)with EPS-LM.This study provided thus further insights into the interactions between EPS-LM and LTB,suggesting that EPS produced by some LAB,such as EPS produced by Ln.mesenteroides P35 strain are good candidates to inhibit E.coli toxin activity.
基金This research was funded by competitive grants from Fondo María Viñas of ANII(Agencia Nacional de Investigación e Innovación,Uruguay)to A.L.,and full-time funds(DT,UDELAR,Uruguay)granted to A.L.
文摘Background Kernza®intermediate wheatgrass is a perennial grain and forage crop that can provide several ecosystem services.Major research efforts focused on Kernza have taken place in high latitudes.The goal of this study was to evaluate,for the first time,the agronomic performance of Kernza in a low-latitude region with mild winters.Methods A KS-cycle 4 Kernza population(A)was planted in spring in Wisconsin,USA,and selected in one cycle for lower vernalization requirements,obtaining a new population(B).These two populations,at three nitrogen(N)fertilization rates,were evaluated in a full factorial,completely randomized field experiment in Uruguay over 2 years.Results The populations were similar in grain yields and flowering time in the 1st year,but population B had 63%lower grain yield in the 2nd year and 20%lower forage yield throughout the experiment.Increasing the N rate to 160 kg ha−1 led to a 63%increase in grain yield and 28%increase in forage yield across populations.Forage yields and nutritive values were similar to those reported in the northern hemisphere.However,grain yields for both the 1st(316 kg ha−1)and 2nd year(41 kg ha−1)were lower due to reduced flowering and weed competition.Conclusions Expansion of Kernza to lower-latitude regions will require further breeding to improve reproductive performance.
