Anthropogenic ammonia emissions primarily originate from agriculture,especially field fertilization.These emissions represent nitrogen loss for farmers and contribute to air pollution,posing risks to human health and ...Anthropogenic ammonia emissions primarily originate from agriculture,especially field fertilization.These emissions represent nitrogen loss for farmers and contribute to air pollution,posing risks to human health and the environment.Estimating ammonia emissions is crucial for national inventories and policy-making.Various models exist for predicting emissions,including mechanistic,empirical,and semi-empirical approaches.While machine learning(ML)is widely used in environmental science,its application to ammonia emissions remains limited.In this study,we used 5939 ammonia emission data from 538 trials,extracted from the ALFAM2 database,to train three machine learning methods-random forest,gradient boosting,and lasso-for predicting cumulative ammonia emissions 72 h after manure application.These methods were compared to the semi-empirical ALFAM2 model using an independent test dataset.Random forest(RMSE=4.51,r=0.94,MAE=3.28,Bias=0.92)and gradient boosting(RMSE=6.19,r=0.89,MAE=4.10,Bias=0.51)showed the best performance,while the lasso log-linear model(RMSE=7.30,r=0.84,MAE=5.57,Bias=-1.38)performed worst.Both random forest and gradient boosting outperformed the semi-empirical ALFAM2 model,which showed performance comparable to the lasso model.We then used these models and the ALFAM2 model to compare five slurry management techniques,varying in application method(trailing hoses,trailing shoes,and open slot)and post-application incorporation,across 128 scenarios with different manure types and weather conditions.Compared to broadcast application,alternative techniques reduced emissions by a median of-13.6%to-61.7%.This study highlights the promise of ML models in assessing ammonia emission reduction methods,while emphasizing the importance of evaluating model sensitivity to algorithm choice.展开更多
Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown dec...Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown declining performance in recent years under intensifying climate stress.Therefore,targeted breeding strategies could discover genotypes suitable for autumn or early winter sowing,with cold tolerance as a key selection criterion.Currently,‘Ichraq'is the only autumn-planted sunflower variety officially registered in Morocco,although efforts to release additional tolerant varieties are underway.This study evaluated 31 genotypes(MGB1to MGB31)selected from various environments under autumn planting conditions and conserved in the Moroccan Gene Bank.These genotypes were planted in early winter at a mountainous site known for its pronounced winter cold.Eighteen Morphological,physiological and agronomic parameters including initial vigor,leaf area,seed yield,oil content etc.,were assessed using both univariate and multivariate statistical approaches.Analysis of variance revealed significant genotypic differences across most traits,indicating substantial genetic variation.Notably,seed oil content ranged from 23.28%(MGB26)to 43.88%(MGB5),and seed yield from1400 kg/ha(MGB7)to 5400 kg/ha(MGB8).Principal component analysis(PCA)identified that the first principal component,accounting for over 24%of the total phenotypic variance,exhibits a strong positive loading of yield-related traits and chlorophyll content,while displaying a pronounced negative loading for oil content variables.This opposing gradient indicates a clear trade-off between vegetative productivity and oil accumulation across the evaluated genotypes.Hierarchical cluster analysis resolved the germplasm into two principal clusters with high within-group similarity,each further partitioned into relatively homogeneous subgroups.Notably,several genotypes outperformed the control variety Ichraq,underscoring their potential for autumn or early winter cultivation.Nonetheless,essential multi-environment trials remain to validate their phenotypic stability and to ascertain their value as genetic resources for sunflower breeding programs in Morocco and other Mediterranean agro-ecosystems.展开更多
Background Sphingolipids(SL)are key regulators of inflammatory processes,yet their roles in dairy cows remain poorly understood.This study investigated the effects of inflammation(plasma haptoglobin concentration),ket...Background Sphingolipids(SL)are key regulators of inflammatory processes,yet their roles in dairy cows remain poorly understood.This study investigated the effects of inflammation(plasma haptoglobin concentration),ketosis,and mastitis on plasma SL profiles in Holstein cows sampled seven days postpartum.From a cohort of 427 cows across 25 farms,80 animals were classified into four groups:inflammation(n=20),ketosis(n=19),mastitis(n=21),and healthy controls(n=20).Plasma SL were quantified by targeted HPLC-MS/MS,while cytokines were quantified with a 15-plex bead-based assay.Both univariate and multivariate analyses were applied to assess pathological effects,along with SL ratios and correlations between SL and cytokines.Results Systemic inflammation detected through the haptoglobin measure induced the most pronounced alterations in SL metabolism,characterized by elevated dihydrosphingomyelins(DHSM)and lactosylceramides(Lac Cer),higher C22-24:C16 ratios,and lower unsaturated:saturated ratios in ceramides(Cer)and sphingomyelins(SM).Although total Cer,SM,and the Cer:SM ratio remained unchanged,specific reductions were observed in both Cer and SM in C14,Cer C18:1,SM C16:1,and SM C23:1,whereas SM C25:0 and C26:0 increased.Sphingosine-1-phosphate(So1P)was positively correlated with IL-10 as well as IL-1α and TNFα,while C18-20 Cer correlated positively with multiple pro-inflammatory cytokines and chemokines such as CXCL8 and CCL2.Ketosis induced subtler changes,primarily an increase in plasma DHSM and DHSM:SM ratio(driven by C16:0),an increase in C22-24:C16 DHCer ratio,and a decrease in both Lac So:Lac Cer and unsaturated:saturated ratios in C23-SM.In this group,So1P correlated positively with CXCL8 and CCL2.Moreover C18-20 Cer and DHCer were positively associated with CXCL8,CCL2,CCL3,and CCL4,which also showed correlations with most Lac Cer species.Analysis of chronic mastitis cases yielded a clear separation from controls in multivariate analysis but only minimal changes in SL concentrations and ratios,maybe due to the localized nature of the inflammatory response.Conclusions In summary,heightened inflammatory response in early post-partum is associated with the strongest systemic effects on SL metabolism,followed by ketosis,while mastitis induced only modest alterations.These findings highlight condition-specific patterns of SL regulation postpartum and suggest potential immunometabolic biomarkers of disease.展开更多
Dear Editor,The long-term use of copper(Cu)fungicides to prevent downy mildew of vine led to the accumulation of Cu in vineyard topsoils(Komárek et al.,2010;Droz et al.,2021),which may alter the functioning and s...Dear Editor,The long-term use of copper(Cu)fungicides to prevent downy mildew of vine led to the accumulation of Cu in vineyard topsoils(Komárek et al.,2010;Droz et al.,2021),which may alter the functioning and sustainability of vineyard ecosystems(Cornu et al.,2022).展开更多
Skeletal muscle accounts for approximately 40%of body mass and 50%–75%of whole-body protein,playing a central role in meat production and quality.Efficient protein synthesis in skeletal muscle relies on an adequate s...Skeletal muscle accounts for approximately 40%of body mass and 50%–75%of whole-body protein,playing a central role in meat production and quality.Efficient protein synthesis in skeletal muscle relies on an adequate supply of nutrient substrates and a balanced amino acid profile.Branched-chain amino acids(BCAA),including leucine(Leu),isoleucine(Ile),and valine(Val),are the most abundant essential amino acids in skeletal muscle and contribute to both protein synthesis and oxidative energy production.Additionally,BCAA function as signaling molecules that regulate gene expression and protein phosphorylation cascades,which significantly influence physiological processes,such as protein synthesis and degradation,glucose and lipid metabolism,and cell apoptosis and autophagy.These processes are primarily mediated through the PI3K/AKT/AMPK/mTOR signaling pathways.This review summarizes BCAA transporters and catabolic metabolism,their role as signaling molecules in regulating protein metabolism and glucose and lipid equilibrium,and applications in animal production.These findings offer both theoretical insights and practical guidelines for the precise regulation of feed efficiency and production performance through tailored dietary BCAA supplementations.展开更多
Morocco's oat sector is shifting from forage to food,creating demand for varieties with proven processing performance.We profiled nine Moroccan oats(four parental lines,four interspecific derivatives,and one hull-...Morocco's oat sector is shifting from forage to food,creating demand for varieties with proven processing performance.We profiled nine Moroccan oats(four parental lines,four interspecific derivatives,and one hull-less diploid check)against the key drivers of functionality:β-glucan,hydration metrics(WAI,WSI,swelling power),interfacial metrics(foam capacity/stability,emulsion capacity/stability),and kernel geometry(thousand-kernel weight/width),using SEM to interpret microstructure.Varietal differences were pronounced and actionable.The A.sativa×A.magna derivative Hamdali showed fast wetting(low WAI),strong foaming(highest FS),and high emulsion capacity.These traits make it suitable for oat drinks and large,crack-free flakes.The A.sativa×A.murphyi descendants Al Fawze and Abtah exhibited restrained swelling(lower SP)and moderate WAI/WSI,favoring crisp snacks,biscuits,and pasta;Abtah additionally delivered high emulsion stability suitable for shelf-stable beverages.Amlal and Nezha offered balanced,steerable profiles.Linkingβ-glucan,hydration,and interfacial behavior to kernel traits provides a variety-to-application map for Moroccan oats.We recommend Hamdali/Niema for foamed beverages/flakes;Tissir/Soualem for porridges and thick beverages;Abtah for pasta and stable emulsions;Al Fawze for crisp snacks/biscuits.展开更多
Monoculture and intensive fertiliser use in the cultivation of Robusta coffee(Coffea canephora var.Robusta)and black pepper(Piper nigrum L.)have led to soil degradation and increased disease pressure in Vietnam's ...Monoculture and intensive fertiliser use in the cultivation of Robusta coffee(Coffea canephora var.Robusta)and black pepper(Piper nigrum L.)have led to soil degradation and increased disease pressure in Vietnam's Central Highlands.To identify key factors driving soilborne diseases and threatening sustainable production,a soil and root survey was conducted across three provinces:Gia Lai,Dak Lak,and Dak Nong.Soils were characterised by high clay content(51.2-62.0%),moderate silt(35.5-46.0%),and low sand(2.5-2.8%),with a notably low cation exchange capacity(7.72-8.04 cmol_(c) kg^(−1)).The soils were strongly acidic,with average pH values of 4.51 in coffee farms and 5.45 in pepper farms.Despite sufficient levels of nitrogen(N),phosphorus(P),and potassium(K),soilborne pathogens were widespread.Fusarium spp.were detected in all samples,Phytophthora spp.in 64-76%of black pepper farms,and plant-parasitic nematodes in 67-84%of farms,with Meloidogyne spp.predominant.Fusarium density increased with soil acidity in coffee plantations.In coffee,nematode density was positively correlated with N input,while in black pepper,N was negatively correlated with Phytophthora.Organic matter and available K were negatively associated with Fusarium in coffee but positively with Phytophthora in black pepper.These findings underscore the need for integrated management of nutrients and pathogens to sustain perennial crop production in the region.展开更多
In Alzheimer’s disease,microglial phagocytosis is engaged in the pathogenesis as it clears abnormal protein accumulations,debris,and apoptotic cells in the early stages of Alzheimer’s disease,but fuels neuroinflamma...In Alzheimer’s disease,microglial phagocytosis is engaged in the pathogenesis as it clears abnormal protein accumulations,debris,and apoptotic cells in the early stages of Alzheimer’s disease,but fuels neuroinflammation and accelerates disease progression in later stages.In vivo parabiosis experiments in aged animals have demonstrated that blood-born factors modulate synaptic plasticity,neurogenesis,and microglial responses.We hypothesize that peripheral factors can modulate microglial function and thereby possibly influence Alzheimer’s disease pathology.The objective of this study is to investigate the effects of Alzheimer’s disease serum on microglial phagocytosis.Here,we use an immortalized human microglial cell line in an in vitro parabiosis assay to investigate the impact of the serum from individuals diagnosed with Alzheimer’s disease(n=30)and age-matched controls(n=30)(PRODEM study)on microglial phagocytosis.Exposure to Alzheimer’s disease serum increased microglial phagocytic uptake of pH-sensitive fluorescent particles and downregulated expression of the lysosomal master regulator transcription factor EB(TFEB)and of ATPase H^(+)transporting lysosomal V1 subunit B2(ATP6V1B2),a component of the vacuolar ATPase.To identify serum components that may relate to changes in phagocytosis,serum samples of the Three-City Study(3C Study)were used.In the 3C Study,blood samples were collected up to 12 years before the onset of cognitive decline or dementia and their serum metabolome is well-defined.Microglia exposed to the serum of future Alzheimer’s disease patients from the 3C Study displayed an increased phagocytic uptake compared with the serum of matched controls,depending on the presence of the apolipoprotein Eε4 allele in the Alzheimer’s disease patients.Furthermore,microglial phagocytosis correlated inversely with serum levels of the omega-3 fatty acid eicosapentaenoic acid.We confirmed this inverse correlation between eicosapentaenoic acid and phagocytosis in the serum samples of the PRODEM cohort.In addition,in vitro testing of eicosapentaenoic acid on microglial phagocytosis showed a concentration-dependent decrease in phagocytic uptake.In conclusion,following incubation with Alzheimer’s disease blood serum,we observed increased microglial phagocytic uptake and the downregulation of TFEB and ATP6V1B2,possibly indicating lysosomal dysfunction.Furthermore,microglial phagocytosis was inversely correlated with serum eicosapentaenoic acid levels,suggesting an important role for dietary eicosapentaenoic acid in microglial function.展开更多
Cadmium(Cd)is a toxic trace metal that occurs naturally in agricultural soils and can accumulate in the edible parts of crops,notably in cereal grains.This study investigated the extent to which sulfur(S)fertilization...Cadmium(Cd)is a toxic trace metal that occurs naturally in agricultural soils and can accumulate in the edible parts of crops,notably in cereal grains.This study investigated the extent to which sulfur(S)fertilization increases the availability of Cd in a calcareous(Calc)and a non-calcareous(Ncalc)soil.Changes in Cd availability and speciation were monitored over a 21-d incubation period,along with changes in pH and the concentrations of sulfate,nitrate,calcium,magnesium,aluminum(Al),manganese(Mn),and nickel(Ni)in the potassium nitrate(KNO_(3))extracts,after the addition at agronomic rates of three forms of S fertilizers:elemental S(S(0)),potassium sulfate(K_(2)SO_(4)),and ammonium sulfate((NH_(4))_(2)SO_(4)).Elemental S did not increase the Cd concentration in the KNO_(3)extracts of either soil due to its too limited oxidation to sulfates.The two sulfate fertilizers had contrasting effects on Cd concentration depending on the nature of the counter cation(K^(+)or NH_(4)^(+)).While K_(2)SO_(4)caused almost no change in Cd concentration in either soil,(NH_(4))_(2)SO_(4)increased Cd concentration in both soils.The nitrification of NH_(4)^(+)reduced soil pH,thereby increasing the concentrations of Cd,Al,Mn,and Ni in the Ncalc soil over time,as evidenced by the strong negative correlations between soil pH and the concentrations of these four elements in the KNO3 extract after 21 d.In the Calc soil,the mechanism of Cd concentration increase after(NH_(4))_(2)SO_(4)addition was more difficult to understand and could result from the proton-mediated alteration of Cd-bearing phases such as carbonates,following ammonia(NH_(3))volatilization.The relevance of these results for the S fertilization of cereals in Cd-contaminated soils is discussed.展开更多
Fusarium graminearum is the causal agent of Gibberella ear rot(GER)in maize,a devastating fungal disease leading to yield reduction and contamination of grains with type B trichothecene mycotoxins(TCTB).Reducing GER d...Fusarium graminearum is the causal agent of Gibberella ear rot(GER)in maize,a devastating fungal disease leading to yield reduction and contamination of grains with type B trichothecene mycotoxins(TCTB).Reducing GER damage requires the implementation of an integrated strategy in which the use of resistant maize genotypes is a key pillar.F.graminearum infects maize ears most often by the silk channel,which represents the first plant tissue encountered by the pathogen.The present study aimed at providing new phenotyping tools to improve breeding pipelines by investing the importance of maize silk composition in GER resistance.Here we used targeted and untargeted metabolomics approaches to characterize differences between genotypes reported as susceptible or tolerant to GER.Promising results were obtained with the LC-HRMS/MS analysis that led to outline 25 m/z signals,among which 14 were assigned to a unique compound,that could distinguish tolerant and susceptible genotypes.We notably evidenced that a metabolite putatively identified as feruloyl-hexose could contribute to GER tolerance,while two others putatively identified compounds(chlorogenic acid and eupatilin)could be susceptibility-associated biomarkers.The present study paves the avenue for the use of new approaches based on silk composition to improve the breeding programs aiming at increasing maize resistance.展开更多
Tannins are polyphenols widely present in the plant kingdom,commonly divided into two groups:condensed and hydrolysable tannins.Sustainable furanic bio-foams based on condensed tannins have been largely studied,but li...Tannins are polyphenols widely present in the plant kingdom,commonly divided into two groups:condensed and hydrolysable tannins.Sustainable furanic bio-foams based on condensed tannins have been largely studied,but little is described about the use of hydrolysable tannins for this material.This study examined the potential of hydrolysable chestnut tannin in comparison to condensed mimosa tannins to produce furanic foams by chemical expansion.Due to the low reactivity of the hydrolysable tannin,the use of an external source for its polymerization and curing was necessary.Through Fourier transform infrared spectroscopy(FTIR)chromatography,it was possible to observe that the new foams presented small differences in functional groups compared to the condensed tannin foams,presenting peaks related to carboxyl groups.In terms of physical properties,the chestnut foams showed an apparent density 36%higher than the conventional mimosa tannin foams and a superior hydrophilic character.In terms of thermal properties,both foams exhibit high thermal stability,with the acacia tannin foam being slightly superior.In summary,this research paves the way for new applications of hydrolysable tannins in bio-foams and materials science.展开更多
The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance...The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance of low-durable wood species.Wacapou(Vouacapoua americana.,Fabaceae)is a well-known Guianese wood spe-cies commonly used in local wood construction due to its outstanding natural durability,which results from the presence of a large panel of extractives compounds.In addition,its industrial processing generates large amounts of residues.Wacapou residues were extracted by maceration using four different solvents(water/ethanol,ethyl acetate,hexane and dichloromethane/methanol),separately and successively.The yield of each extractive fraction was determined,and their chemical compositions were analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS).Ethyl acetate led to the highest extraction yield,and the active compounds were identified in the obtained extractive fraction.In this sense,the fungicidal and termite-repellent properties of these extractives were then tested using a screening laboratory(with temperate and tropical microorganisms),according to the solution concentration(1%,2.5%,5%,8%and 10%).Finally,Virola michelii Heckel wood samples(low durable species)were impregnated with the 8%concentration solution.The impregnated wood samples were then exposed to a soil bed test.The results highlighted that the nature of the solvent used during wood maceration affects the con-tent of the obtained extractive fractions.Ultra-Performance Liquid Chromatography–High-Resolution Mass Spectrometry(UHPLC-HRMS)analyses showed the influence of extraction parameters on the nature of the extracted molecules.Wacapou extracts(from ethyl acetate maceration)showed good anti-fungal and anti-termite activities.Additionally,the concentration in extractives had an impact on the anti-termite activity level for Reti-culitermesflavipes and Cryptotermes sp.Formulations based on Wacapou extractives showed a good potential for valorization in eco-friendly preservatives,aiming to confer better durability to local low-durability wood species.展开更多
Bio-based thermoplastic film from flax fiber and fatty acid(FA)was obtained using trifluoroacetic anhydride(TFAA)as an impelling agent.Different quantities of TFAA/FA,size of flax fiber,and fatty acids were applied to...Bio-based thermoplastic film from flax fiber and fatty acid(FA)was obtained using trifluoroacetic anhydride(TFAA)as an impelling agent.Different quantities of TFAA/FA,size of flax fiber,and fatty acids were applied to investigate chemical structure in relation to the mechanical properties.Decreasing the quantity of TFAA/FA by almost half from 1:4 to 1:2.5(flax to TFAA/FA)only reduces by 22%the weight percent gain(WPG)and ester content and reducing flax fiber size slightly increases the WPG and ester content.All the treatments showed sig-nificant chemical structure modification,observed by FTIR and solid CP/MAS^(13)C NMR,confirming the presence of carbonyl ester groups and alkyl chains,in relatively similar intensities.The crystallinity index(CrI)of esterified flax was evaluated by comparing the signal of solid CP/MAS^(13)C NMR in crystalline and amorphous regions and CrI was higher in esterified flax using a lower quantity of reagent and longer fatty acid.Esterified flax in a high quantity of reagent showed ductile or flexible behavior.Decreasing the reagent to 1:2.5 significantly increases the tensile strength and Young’s modulus,and decreases the elongation at break,presenting more brittle and stiff material.Using flax fiber in the original size results in slightly higher tensile strength and Young’s modulus and slightly lower elongation than milled flax.The tensile strength and Young’s modulus of stearic acid esterified flax obtained in this research were higher than myristic acid and comparable to the polyethylene plastics-LDPE and HDPE.展开更多
Background Insect meals have been identified as innovative and sustainable feedstuffs that could be used in ruminant nutrition.However,current research on the effects that their processing may have on rumen digestibil...Background Insect meals have been identified as innovative and sustainable feedstuffs that could be used in ruminant nutrition.However,current research on the effects that their processing may have on rumen digestibility and fatty acid(FA)biohydrogenation is scant.This trial aims to investigate the effects(i)of drying temperature of fullfat Hermetia illucens(HI)and Tenebrio molitor(TM)meals,and(ii)of residual ether extract(EE)content of defatted HI and TM meals,on their fermentation characteristics and FA of rumen digesta after 24-h in vitro rumen incubation.Methods The tested full-fat meals included four HI and four TM meals obtained applying drying temperatures ranging from 30℃ to 70℃,while the tested defatted meals consisted of five HI and two TM meals containing a residual EE content ranging from 4.7 to 19.7 g EE/100 g dry matter(DM).The applied statistical models(GLM ANOVA)tested the effects of insect species,drying temperature(full-fat meals)or EE content(defatted meals),and their interaction.Results Drying temperature had minor effects on in vitro ruminal digestibility and FA profile of rumen digesta.Irrespective of insect species,increasing the drying temperature led to a reduction of in vitro degradation of proteins from insect meals,as outlined by the significant decrease in ammonia production(-0.009 mmol/g DM and-0.126 g/100 g total N for each additional 1℃).Irrespective of insect species,defatting increased total gas,volatile fatty acids(VFA)and CH_(4) productions,and the proportions of total saturated and branched-chain FA in rumen digesta(+0.038 mmol/g DM,+0.063 mmol/g DM,+12.9μmol/g DM,+0.18 g/100 g FA,and+0.19 g/100 g FA for each reduced 1 g EE/100 g DM,respectively),and reduced the proportion of total PUFA(-0.12 g/100 g FA).Conclusions The applied drying temperatures of full-fat insect meals are too low to exert impactful effects on rumen digestibility and FA biohydrogenation.Fat lowered fermentation activity,probably because of an inhibitory effect on rumen microbiota.The increased ruminal digestibility of defatted insect meals suggests that they can be more suitable to be used in ruminant nutrition than full-fat ones.展开更多
As a major subunit of the exocyst complex,members of the EXO70 family have mainly been shown to play roles in cell polarity and morphogenesis in Arabidopsis,but their roles in plant endosymbiosis,such as with arbuscul...As a major subunit of the exocyst complex,members of the EXO70 family have mainly been shown to play roles in cell polarity and morphogenesis in Arabidopsis,but their roles in plant endosymbiosis,such as with arbuscular mycorrhizal fungi(AMF),have rarely been reported.Here,using knockout and overexpression lines,we show that OsEXO70L2,which encodes a divergent EXO70 protein in rice,controls the number of primary roots and is essential for large lateral root formation.Furthermore,the OsEXO70L2 mutant sr1 displayed rare internal AMF hyphaeand no arbuscules.We also found that AMF sporulation can occur in roots despite low colonization and that AMF colonization and sporulation are modulated by photoperiod and co-culture with clover.Finally,genes related to auxin homeostasis were found to be affected in the OsEXO70L2 knockout or overexpression lines,suggesting that auxin is at least partly responsible for the phenotypes.This study provides new perspectives on the role of the exocyst complex during root development and AM in rice.展开更多
Background Agroecological cropping systems are recognised as an alternative way to ensure the sustainability of cotton(Gossypium hirsutum L.) production in the context of climate change and degradation of soil fertili...Background Agroecological cropping systems are recognised as an alternative way to ensure the sustainability of cotton(Gossypium hirsutum L.) production in the context of climate change and degradation of soil fertility. A study was conducted in Benin from 2020 to 2023 to compare six different cotton cultivars in three agroecological cropping systems in two cotton-growing zones. Plough-based tillage plus incorporation of cover crop biomass(PTI), conservation agriculture with strip tillage(CA_ST), and conservation agriculture with no tillage(CA_NT) were compared with the reference plough-based tillage(PT). The objective was to identify morpho-physiological traits of cotton that increase yield in agroecological cropping systems. Our approach combined a field experiment and crop simulation model(CSM) of CROPGRO-Cotton to evaluate the effects of genotype(G) × environment(E) × management(M) interactions on seed cotton yield(SCY).Results Cultivars Tamcot_camde and Okp768 and simulated ideotypes performed best in CA systems. Increased seed mass, large and thick leaves, and later maturity were identified as beneficial for yield enhancement in CA systems. Cultivars and ideotypes that combine these traits also resulted in better nitrogen and water use efficiencies in CA systems. Under different climate scenarios up to 2050, ideotypes designed could increase SCY in Benin.Conclusion A set of morpho-physiological traits associated with vegetative vigour is required to ensure a good SCY in agroecological cropping systems. These results provide scientific evidence and useful knowledge for breeders and research programmes on cropping systems focused on the adaptation of cotton to climate change.展开更多
Furfurylation,a renowned chemical modification technique,uses furfuryl alcohol to enhance the properties of wood.This technology can be further refined by incorporating renewable tannins,which promote cross-linking wi...Furfurylation,a renowned chemical modification technique,uses furfuryl alcohol to enhance the properties of wood.This technology can be further refined by incorporating renewable tannins,which promote cross-linking with furfuryl alcohol.This study investigates the effects of furfurylation and tannin-modified furfurylation on the physical and mechanical properties of tropical Gmelina wood(Gmelina arborea Roxb.).Experiments involved impregnating Gmelina wood with aqueous solutions of furfuryl alcohol(FA)at 40%and 70%concentrations,as well as FA-tannin combinations(FA 40%-TA and FA 70%-TA),followed by polymerization at 103℃.The results demonstrated that both FA and FA-tannin treatments significantly improved the wood’s physical and mechanical properties.Notably,FA-tannin treatments achieved anti-swelling efficiency comparable to FA alone.However,the addition of tannins(FA 70%-TA)enhanced leaching resistance by up to 47%,contributing to a more environmentally sustainable modification process.Mechanically,the inclusion of tannins in FA 70%-TA slightly increased the modulus of elasticity(14%~8732 kg/cm^(2))and the modulus of rupture(9%~40.9 kg/cm^(2)).Furthermore,the tannin addition imparted a darker color to the modified wood,enhancing its aesthetic appeal.This study highlights the potential of tannin-modified furfurylation to advance wood modification technology,combining improved performance with environmental benefits.展开更多
基金the French state aid managed by the ANR under the“Investissements d’avenir”programme with the reference ANR-16-CONV-0003from the AgroEcoSystem department of INRAE.We are grateful to the INRAE MIGALE bioinformatics facility(MIGALE,INRAE,2020.Migale bioinformatics Facility,doi:10.15454/1.5572390655343293E12)for providing help and/or computing and/or storage resources.We are also grateful to Sasha Hafner for his help in reproducing some of the results of Hafner et al.(2019).
文摘Anthropogenic ammonia emissions primarily originate from agriculture,especially field fertilization.These emissions represent nitrogen loss for farmers and contribute to air pollution,posing risks to human health and the environment.Estimating ammonia emissions is crucial for national inventories and policy-making.Various models exist for predicting emissions,including mechanistic,empirical,and semi-empirical approaches.While machine learning(ML)is widely used in environmental science,its application to ammonia emissions remains limited.In this study,we used 5939 ammonia emission data from 538 trials,extracted from the ALFAM2 database,to train three machine learning methods-random forest,gradient boosting,and lasso-for predicting cumulative ammonia emissions 72 h after manure application.These methods were compared to the semi-empirical ALFAM2 model using an independent test dataset.Random forest(RMSE=4.51,r=0.94,MAE=3.28,Bias=0.92)and gradient boosting(RMSE=6.19,r=0.89,MAE=4.10,Bias=0.51)showed the best performance,while the lasso log-linear model(RMSE=7.30,r=0.84,MAE=5.57,Bias=-1.38)performed worst.Both random forest and gradient boosting outperformed the semi-empirical ALFAM2 model,which showed performance comparable to the lasso model.We then used these models and the ALFAM2 model to compare five slurry management techniques,varying in application method(trailing hoses,trailing shoes,and open slot)and post-application incorporation,across 128 scenarios with different manure types and weather conditions.Compared to broadcast application,alternative techniques reduced emissions by a median of-13.6%to-61.7%.This study highlights the promise of ML models in assessing ammonia emission reduction methods,while emphasizing the importance of evaluating model sensitivity to algorithm choice.
文摘Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown declining performance in recent years under intensifying climate stress.Therefore,targeted breeding strategies could discover genotypes suitable for autumn or early winter sowing,with cold tolerance as a key selection criterion.Currently,‘Ichraq'is the only autumn-planted sunflower variety officially registered in Morocco,although efforts to release additional tolerant varieties are underway.This study evaluated 31 genotypes(MGB1to MGB31)selected from various environments under autumn planting conditions and conserved in the Moroccan Gene Bank.These genotypes were planted in early winter at a mountainous site known for its pronounced winter cold.Eighteen Morphological,physiological and agronomic parameters including initial vigor,leaf area,seed yield,oil content etc.,were assessed using both univariate and multivariate statistical approaches.Analysis of variance revealed significant genotypic differences across most traits,indicating substantial genetic variation.Notably,seed oil content ranged from 23.28%(MGB26)to 43.88%(MGB5),and seed yield from1400 kg/ha(MGB7)to 5400 kg/ha(MGB8).Principal component analysis(PCA)identified that the first principal component,accounting for over 24%of the total phenotypic variance,exhibits a strong positive loading of yield-related traits and chlorophyll content,while displaying a pronounced negative loading for oil content variables.This opposing gradient indicates a clear trade-off between vegetative productivity and oil accumulation across the evaluated genotypes.Hierarchical cluster analysis resolved the germplasm into two principal clusters with high within-group similarity,each further partitioned into relatively homogeneous subgroups.Notably,several genotypes outperformed the control variety Ichraq,underscoring their potential for autumn or early winter cultivation.Nonetheless,essential multi-environment trials remain to validate their phenotypic stability and to ascertain their value as genetic resources for sunflower breeding programs in Morocco and other Mediterranean agro-ecosystems.
基金partly funded by APIS-GENEsupported by a grant Bonus QualitéRecherche(BQR)s from ENVT。
文摘Background Sphingolipids(SL)are key regulators of inflammatory processes,yet their roles in dairy cows remain poorly understood.This study investigated the effects of inflammation(plasma haptoglobin concentration),ketosis,and mastitis on plasma SL profiles in Holstein cows sampled seven days postpartum.From a cohort of 427 cows across 25 farms,80 animals were classified into four groups:inflammation(n=20),ketosis(n=19),mastitis(n=21),and healthy controls(n=20).Plasma SL were quantified by targeted HPLC-MS/MS,while cytokines were quantified with a 15-plex bead-based assay.Both univariate and multivariate analyses were applied to assess pathological effects,along with SL ratios and correlations between SL and cytokines.Results Systemic inflammation detected through the haptoglobin measure induced the most pronounced alterations in SL metabolism,characterized by elevated dihydrosphingomyelins(DHSM)and lactosylceramides(Lac Cer),higher C22-24:C16 ratios,and lower unsaturated:saturated ratios in ceramides(Cer)and sphingomyelins(SM).Although total Cer,SM,and the Cer:SM ratio remained unchanged,specific reductions were observed in both Cer and SM in C14,Cer C18:1,SM C16:1,and SM C23:1,whereas SM C25:0 and C26:0 increased.Sphingosine-1-phosphate(So1P)was positively correlated with IL-10 as well as IL-1α and TNFα,while C18-20 Cer correlated positively with multiple pro-inflammatory cytokines and chemokines such as CXCL8 and CCL2.Ketosis induced subtler changes,primarily an increase in plasma DHSM and DHSM:SM ratio(driven by C16:0),an increase in C22-24:C16 DHCer ratio,and a decrease in both Lac So:Lac Cer and unsaturated:saturated ratios in C23-SM.In this group,So1P correlated positively with CXCL8 and CCL2.Moreover C18-20 Cer and DHCer were positively associated with CXCL8,CCL2,CCL3,and CCL4,which also showed correlations with most Lac Cer species.Analysis of chronic mastitis cases yielded a clear separation from controls in multivariate analysis but only minimal changes in SL concentrations and ratios,maybe due to the localized nature of the inflammatory response.Conclusions In summary,heightened inflammatory response in early post-partum is associated with the strongest systemic effects on SL metabolism,followed by ketosis,while mastitis induced only modest alterations.These findings highlight condition-specific patterns of SL regulation postpartum and suggest potential immunometabolic biomarkers of disease.
基金financially supported by the Bordeaux Wine Interprofessional Council(French acronym CIVB)in the framework of the EXTRACUIVRE projectby the French National Research Institute for Agriculture,Food and Environment(INRAE)in the framework of the COPOFTEA projectpartially supported by the TSU Program Priority 2030,Russia。
文摘Dear Editor,The long-term use of copper(Cu)fungicides to prevent downy mildew of vine led to the accumulation of Cu in vineyard topsoils(Komárek et al.,2010;Droz et al.,2021),which may alter the functioning and sustainability of vineyard ecosystems(Cornu et al.,2022).
基金partly funded by National Key R&D Program of China(2023YFD1301405)the 2115 Talent Development Program of China Agricultural University。
文摘Skeletal muscle accounts for approximately 40%of body mass and 50%–75%of whole-body protein,playing a central role in meat production and quality.Efficient protein synthesis in skeletal muscle relies on an adequate supply of nutrient substrates and a balanced amino acid profile.Branched-chain amino acids(BCAA),including leucine(Leu),isoleucine(Ile),and valine(Val),are the most abundant essential amino acids in skeletal muscle and contribute to both protein synthesis and oxidative energy production.Additionally,BCAA function as signaling molecules that regulate gene expression and protein phosphorylation cascades,which significantly influence physiological processes,such as protein synthesis and degradation,glucose and lipid metabolism,and cell apoptosis and autophagy.These processes are primarily mediated through the PI3K/AKT/AMPK/mTOR signaling pathways.This review summarizes BCAA transporters and catabolic metabolism,their role as signaling molecules in regulating protein metabolism and glucose and lipid equilibrium,and applications in animal production.These findings offer both theoretical insights and practical guidelines for the precise regulation of feed efficiency and production performance through tailored dietary BCAA supplementations.
文摘Morocco's oat sector is shifting from forage to food,creating demand for varieties with proven processing performance.We profiled nine Moroccan oats(four parental lines,four interspecific derivatives,and one hull-less diploid check)against the key drivers of functionality:β-glucan,hydration metrics(WAI,WSI,swelling power),interfacial metrics(foam capacity/stability,emulsion capacity/stability),and kernel geometry(thousand-kernel weight/width),using SEM to interpret microstructure.Varietal differences were pronounced and actionable.The A.sativa×A.magna derivative Hamdali showed fast wetting(low WAI),strong foaming(highest FS),and high emulsion capacity.These traits make it suitable for oat drinks and large,crack-free flakes.The A.sativa×A.murphyi descendants Al Fawze and Abtah exhibited restrained swelling(lower SP)and moderate WAI/WSI,favoring crisp snacks,biscuits,and pasta;Abtah additionally delivered high emulsion stability suitable for shelf-stable beverages.Amlal and Nezha offered balanced,steerable profiles.Linkingβ-glucan,hydration,and interfacial behavior to kernel traits provides a variety-to-application map for Moroccan oats.We recommend Hamdali/Niema for foamed beverages/flakes;Tissir/Soualem for porridges and thick beverages;Abtah for pasta and stable emulsions;Al Fawze for crisp snacks/biscuits.
基金funded by the Australian Centre for International Agricultural Research(ACIAR)project“AGB-2018-175:Enhancing smallholder livelihoods in the Central Highlands of Viet Nam through improving the sustainability of coffee and black pepper farming systems and value chains”and Deakin University.
文摘Monoculture and intensive fertiliser use in the cultivation of Robusta coffee(Coffea canephora var.Robusta)and black pepper(Piper nigrum L.)have led to soil degradation and increased disease pressure in Vietnam's Central Highlands.To identify key factors driving soilborne diseases and threatening sustainable production,a soil and root survey was conducted across three provinces:Gia Lai,Dak Lak,and Dak Nong.Soils were characterised by high clay content(51.2-62.0%),moderate silt(35.5-46.0%),and low sand(2.5-2.8%),with a notably low cation exchange capacity(7.72-8.04 cmol_(c) kg^(−1)).The soils were strongly acidic,with average pH values of 4.51 in coffee farms and 5.45 in pepper farms.Despite sufficient levels of nitrogen(N),phosphorus(P),and potassium(K),soilborne pathogens were widespread.Fusarium spp.were detected in all samples,Phytophthora spp.in 64-76%of black pepper farms,and plant-parasitic nematodes in 67-84%of farms,with Meloidogyne spp.predominant.Fusarium density increased with soil acidity in coffee plantations.In coffee,nematode density was positively correlated with N input,while in black pepper,N was negatively correlated with Phytophthora.Organic matter and available K were negatively associated with Fusarium in coffee but positively with Phytophthora in black pepper.These findings underscore the need for integrated management of nutrients and pathogens to sustain perennial crop production in the region.
基金part of the EU consortium DCog Plast ‘Diet Cognition and Plasticity” funded by the Joint Programming Initiative “A Health Diet for a Healthy Life”(JPI-HDHL) via the BMWFW (BMWFW-10.420/0009-WF/V/3c/2015 and the Medical Research Council UK:MR/N030087/1)(to LA and ST)supported by the PMU-FFF Research Fund (A-16/01/019-AIG)+9 种基金BA by the PMU-Research and Innovation Fund (PMU-RIF)(project 2023-PRE-008-Altendorfer)supported by the Center for Urban Mental Healthby Alzheimer Nederlandthe Zon MW Program Mechanisms Of DEMentia (MODEM)by the Gravitation program iCNS of the Dutch Research Council (NWO)supported by Grant PID2020-114921RB-C21Maria de Maeztu Unit of Excellence grant CEX2021-001234-M funded by MCIU/AEI/and CIBERFESCB16/10/00269, from the Instituto de Salud Carlos III all of them by “ERDF A way of making Europe”the Generalitat de Catalunya’s Agency AGAUR of 2021SGR00687ICREA Award
文摘In Alzheimer’s disease,microglial phagocytosis is engaged in the pathogenesis as it clears abnormal protein accumulations,debris,and apoptotic cells in the early stages of Alzheimer’s disease,but fuels neuroinflammation and accelerates disease progression in later stages.In vivo parabiosis experiments in aged animals have demonstrated that blood-born factors modulate synaptic plasticity,neurogenesis,and microglial responses.We hypothesize that peripheral factors can modulate microglial function and thereby possibly influence Alzheimer’s disease pathology.The objective of this study is to investigate the effects of Alzheimer’s disease serum on microglial phagocytosis.Here,we use an immortalized human microglial cell line in an in vitro parabiosis assay to investigate the impact of the serum from individuals diagnosed with Alzheimer’s disease(n=30)and age-matched controls(n=30)(PRODEM study)on microglial phagocytosis.Exposure to Alzheimer’s disease serum increased microglial phagocytic uptake of pH-sensitive fluorescent particles and downregulated expression of the lysosomal master regulator transcription factor EB(TFEB)and of ATPase H^(+)transporting lysosomal V1 subunit B2(ATP6V1B2),a component of the vacuolar ATPase.To identify serum components that may relate to changes in phagocytosis,serum samples of the Three-City Study(3C Study)were used.In the 3C Study,blood samples were collected up to 12 years before the onset of cognitive decline or dementia and their serum metabolome is well-defined.Microglia exposed to the serum of future Alzheimer’s disease patients from the 3C Study displayed an increased phagocytic uptake compared with the serum of matched controls,depending on the presence of the apolipoprotein Eε4 allele in the Alzheimer’s disease patients.Furthermore,microglial phagocytosis correlated inversely with serum levels of the omega-3 fatty acid eicosapentaenoic acid.We confirmed this inverse correlation between eicosapentaenoic acid and phagocytosis in the serum samples of the PRODEM cohort.In addition,in vitro testing of eicosapentaenoic acid on microglial phagocytosis showed a concentration-dependent decrease in phagocytic uptake.In conclusion,following incubation with Alzheimer’s disease blood serum,we observed increased microglial phagocytic uptake and the downregulation of TFEB and ATP6V1B2,possibly indicating lysosomal dysfunction.Furthermore,microglial phagocytosis was inversely correlated with serum eicosapentaenoic acid levels,suggesting an important role for dietary eicosapentaenoic acid in microglial function.
基金supported by the CNRS-INSU EC2CO program in the framework of the CADSOU project,by the Nouvelle Aquitaine Region in the framework of the ESTOCADE project,and by the French National Research Institute for Agriculture,Food and Environment(INRAE)The authors are grateful to Ms.Melike Emet(INRAE,UMR ISPA)and Ms.Sylvie Bussière(INRAE,UMR ISPA)for their help treating the samples,and to Ms.Francesca Degan(ARVALIS-Institut du végétal)and Ms.Séverine Piutti(INRAE,UMR LAE)for helpful comments.
文摘Cadmium(Cd)is a toxic trace metal that occurs naturally in agricultural soils and can accumulate in the edible parts of crops,notably in cereal grains.This study investigated the extent to which sulfur(S)fertilization increases the availability of Cd in a calcareous(Calc)and a non-calcareous(Ncalc)soil.Changes in Cd availability and speciation were monitored over a 21-d incubation period,along with changes in pH and the concentrations of sulfate,nitrate,calcium,magnesium,aluminum(Al),manganese(Mn),and nickel(Ni)in the potassium nitrate(KNO_(3))extracts,after the addition at agronomic rates of three forms of S fertilizers:elemental S(S(0)),potassium sulfate(K_(2)SO_(4)),and ammonium sulfate((NH_(4))_(2)SO_(4)).Elemental S did not increase the Cd concentration in the KNO_(3)extracts of either soil due to its too limited oxidation to sulfates.The two sulfate fertilizers had contrasting effects on Cd concentration depending on the nature of the counter cation(K^(+)or NH_(4)^(+)).While K_(2)SO_(4)caused almost no change in Cd concentration in either soil,(NH_(4))_(2)SO_(4)increased Cd concentration in both soils.The nitrification of NH_(4)^(+)reduced soil pH,thereby increasing the concentrations of Cd,Al,Mn,and Ni in the Ncalc soil over time,as evidenced by the strong negative correlations between soil pH and the concentrations of these four elements in the KNO3 extract after 21 d.In the Calc soil,the mechanism of Cd concentration increase after(NH_(4))_(2)SO_(4)addition was more difficult to understand and could result from the proton-mediated alteration of Cd-bearing phases such as carbonates,following ammonia(NH_(3))volatilization.The relevance of these results for the S fertilization of cereals in Cd-contaminated soils is discussed.
基金supported by a collaborative project involving INRAE/UR1264 Mycology and Food Safety,France and Syngenta,France(grant agreement 22001389-00004520).
文摘Fusarium graminearum is the causal agent of Gibberella ear rot(GER)in maize,a devastating fungal disease leading to yield reduction and contamination of grains with type B trichothecene mycotoxins(TCTB).Reducing GER damage requires the implementation of an integrated strategy in which the use of resistant maize genotypes is a key pillar.F.graminearum infects maize ears most often by the silk channel,which represents the first plant tissue encountered by the pathogen.The present study aimed at providing new phenotyping tools to improve breeding pipelines by investing the importance of maize silk composition in GER resistance.Here we used targeted and untargeted metabolomics approaches to characterize differences between genotypes reported as susceptible or tolerant to GER.Promising results were obtained with the LC-HRMS/MS analysis that led to outline 25 m/z signals,among which 14 were assigned to a unique compound,that could distinguish tolerant and susceptible genotypes.We notably evidenced that a metabolite putatively identified as feruloyl-hexose could contribute to GER tolerance,while two others putatively identified compounds(chlorogenic acid and eupatilin)could be susceptibility-associated biomarkers.The present study paves the avenue for the use of new approaches based on silk composition to improve the breeding programs aiming at increasing maize resistance.
基金supported by a grant overseen by the French National Research Agency(ANR),as part of the“Investissements d’Avenir”program(ANR-11-LABX-0002-01,Lab of Excellence ARBRE)supported by“Lorraine Universitéd’Excellence”Master Grant,ORION program and by the French Ministry of Foreign Office through EIFFEL program.
文摘Tannins are polyphenols widely present in the plant kingdom,commonly divided into two groups:condensed and hydrolysable tannins.Sustainable furanic bio-foams based on condensed tannins have been largely studied,but little is described about the use of hydrolysable tannins for this material.This study examined the potential of hydrolysable chestnut tannin in comparison to condensed mimosa tannins to produce furanic foams by chemical expansion.Due to the low reactivity of the hydrolysable tannin,the use of an external source for its polymerization and curing was necessary.Through Fourier transform infrared spectroscopy(FTIR)chromatography,it was possible to observe that the new foams presented small differences in functional groups compared to the condensed tannin foams,presenting peaks related to carboxyl groups.In terms of physical properties,the chestnut foams showed an apparent density 36%higher than the conventional mimosa tannin foams and a superior hydrophilic character.In terms of thermal properties,both foams exhibit high thermal stability,with the acacia tannin foam being slightly superior.In summary,this research paves the way for new applications of hydrolysable tannins in bio-foams and materials science.
基金PROTEXTWOOD (ID 2202-102) funded through LabEx AGRO ANR-10-LABX-0001-01 (under ISiteUniversité de Montpellier framework)the project PANTHER2-Guyane funded through AgenceNationale de la Recherche (ANR-22-CE43-0019)+2 种基金“Investissement d’Avenir” grant managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01)supported by the FEDER (European Regional Development Fund)research project “EcovaloBois” (Project number: GY0015430)by the CNRS peps INSIS2018 research project “GuyavaloFibres”.
文摘The valorization of Amazonian wood residues into active chemical compounds could be an eco-friendly,cost-effective and valuable way to develop wood preservative formulations to enhance the decay and termite resistance of low-durable wood species.Wacapou(Vouacapoua americana.,Fabaceae)is a well-known Guianese wood spe-cies commonly used in local wood construction due to its outstanding natural durability,which results from the presence of a large panel of extractives compounds.In addition,its industrial processing generates large amounts of residues.Wacapou residues were extracted by maceration using four different solvents(water/ethanol,ethyl acetate,hexane and dichloromethane/methanol),separately and successively.The yield of each extractive fraction was determined,and their chemical compositions were analyzed by Liquid Chromatography-Mass Spectrometry(LC-MS).Ethyl acetate led to the highest extraction yield,and the active compounds were identified in the obtained extractive fraction.In this sense,the fungicidal and termite-repellent properties of these extractives were then tested using a screening laboratory(with temperate and tropical microorganisms),according to the solution concentration(1%,2.5%,5%,8%and 10%).Finally,Virola michelii Heckel wood samples(low durable species)were impregnated with the 8%concentration solution.The impregnated wood samples were then exposed to a soil bed test.The results highlighted that the nature of the solvent used during wood maceration affects the con-tent of the obtained extractive fractions.Ultra-Performance Liquid Chromatography–High-Resolution Mass Spectrometry(UHPLC-HRMS)analyses showed the influence of extraction parameters on the nature of the extracted molecules.Wacapou extracts(from ethyl acetate maceration)showed good anti-fungal and anti-termite activities.Additionally,the concentration in extractives had an impact on the anti-termite activity level for Reti-culitermesflavipes and Cryptotermes sp.Formulations based on Wacapou extractives showed a good potential for valorization in eco-friendly preservatives,aiming to confer better durability to local low-durability wood species.
基金UR 4370 LERMAB is supported by a grant overseen by the French National Research Agency(ANR)as part of the“Investissements d’Avenir”program(ANR-11-LABX-0002-01,Lab of Excellence ARBRE)in the frame of the project“Woodstic”ICEEL for the financial in the frame of the project“BoisPlast”(CARN 001301).
文摘Bio-based thermoplastic film from flax fiber and fatty acid(FA)was obtained using trifluoroacetic anhydride(TFAA)as an impelling agent.Different quantities of TFAA/FA,size of flax fiber,and fatty acids were applied to investigate chemical structure in relation to the mechanical properties.Decreasing the quantity of TFAA/FA by almost half from 1:4 to 1:2.5(flax to TFAA/FA)only reduces by 22%the weight percent gain(WPG)and ester content and reducing flax fiber size slightly increases the WPG and ester content.All the treatments showed sig-nificant chemical structure modification,observed by FTIR and solid CP/MAS^(13)C NMR,confirming the presence of carbonyl ester groups and alkyl chains,in relatively similar intensities.The crystallinity index(CrI)of esterified flax was evaluated by comparing the signal of solid CP/MAS^(13)C NMR in crystalline and amorphous regions and CrI was higher in esterified flax using a lower quantity of reagent and longer fatty acid.Esterified flax in a high quantity of reagent showed ductile or flexible behavior.Decreasing the reagent to 1:2.5 significantly increases the tensile strength and Young’s modulus,and decreases the elongation at break,presenting more brittle and stiff material.Using flax fiber in the original size results in slightly higher tensile strength and Young’s modulus and slightly lower elongation than milled flax.The tensile strength and Young’s modulus of stearic acid esterified flax obtained in this research were higher than myristic acid and comparable to the polyethylene plastics-LDPE and HDPE.
基金partially granted by the Hi-Ga project,which is part of the programme of the Green ERA-Hub that has received funding from the European Union’s Horizon Europe research and innovation programme under Grant Agreement No.101056828self-financed by the DINAMIC team of the UMR Herbivores(INRAE,VetAgro Sup,Saint-Genès-Champanelle,France)。
文摘Background Insect meals have been identified as innovative and sustainable feedstuffs that could be used in ruminant nutrition.However,current research on the effects that their processing may have on rumen digestibility and fatty acid(FA)biohydrogenation is scant.This trial aims to investigate the effects(i)of drying temperature of fullfat Hermetia illucens(HI)and Tenebrio molitor(TM)meals,and(ii)of residual ether extract(EE)content of defatted HI and TM meals,on their fermentation characteristics and FA of rumen digesta after 24-h in vitro rumen incubation.Methods The tested full-fat meals included four HI and four TM meals obtained applying drying temperatures ranging from 30℃ to 70℃,while the tested defatted meals consisted of five HI and two TM meals containing a residual EE content ranging from 4.7 to 19.7 g EE/100 g dry matter(DM).The applied statistical models(GLM ANOVA)tested the effects of insect species,drying temperature(full-fat meals)or EE content(defatted meals),and their interaction.Results Drying temperature had minor effects on in vitro ruminal digestibility and FA profile of rumen digesta.Irrespective of insect species,increasing the drying temperature led to a reduction of in vitro degradation of proteins from insect meals,as outlined by the significant decrease in ammonia production(-0.009 mmol/g DM and-0.126 g/100 g total N for each additional 1℃).Irrespective of insect species,defatting increased total gas,volatile fatty acids(VFA)and CH_(4) productions,and the proportions of total saturated and branched-chain FA in rumen digesta(+0.038 mmol/g DM,+0.063 mmol/g DM,+12.9μmol/g DM,+0.18 g/100 g FA,and+0.19 g/100 g FA for each reduced 1 g EE/100 g DM,respectively),and reduced the proportion of total PUFA(-0.12 g/100 g FA).Conclusions The applied drying temperatures of full-fat insect meals are too low to exert impactful effects on rumen digestibility and FA biohydrogenation.Fat lowered fermentation activity,probably because of an inhibitory effect on rumen microbiota.The increased ruminal digestibility of defatted insect meals suggests that they can be more suitable to be used in ruminant nutrition than full-fat ones.
基金supported by the National Natural Science Foundation of China(32171970)the Chongqing Talent Program,China(cstc2022ycjh-bgzxm0073)+1 种基金the Natural Science Foundation of Chongqing,China(cstc2021jcyjcxtt X0004)the Rice Innovative Research Team of Chongqing Modern Agricultural Industrial Technology System,China(CQMAITS202301)。
文摘As a major subunit of the exocyst complex,members of the EXO70 family have mainly been shown to play roles in cell polarity and morphogenesis in Arabidopsis,but their roles in plant endosymbiosis,such as with arbuscular mycorrhizal fungi(AMF),have rarely been reported.Here,using knockout and overexpression lines,we show that OsEXO70L2,which encodes a divergent EXO70 protein in rice,controls the number of primary roots and is essential for large lateral root formation.Furthermore,the OsEXO70L2 mutant sr1 displayed rare internal AMF hyphaeand no arbuscules.We also found that AMF sporulation can occur in roots despite low colonization and that AMF colonization and sporulation are modulated by photoperiod and co-culture with clover.Finally,genes related to auxin homeostasis were found to be affected in the OsEXO70L2 knockout or overexpression lines,suggesting that auxin is at least partly responsible for the phenotypes.This study provides new perspectives on the role of the exocyst complex during root development and AM in rice.
基金supported by the Benin Cotton Research Institute (IRC)the Cotton Interprofessional Association (AIC)+1 种基金the French Agricultural Research Centre for International Development (CIRAD)the TAZCO_(2) project (Transition Agroécologique des Zones Cotonnières du Bénin),which is funded by the Republic of Benin and the French Development Agency (AFD)。
文摘Background Agroecological cropping systems are recognised as an alternative way to ensure the sustainability of cotton(Gossypium hirsutum L.) production in the context of climate change and degradation of soil fertility. A study was conducted in Benin from 2020 to 2023 to compare six different cotton cultivars in three agroecological cropping systems in two cotton-growing zones. Plough-based tillage plus incorporation of cover crop biomass(PTI), conservation agriculture with strip tillage(CA_ST), and conservation agriculture with no tillage(CA_NT) were compared with the reference plough-based tillage(PT). The objective was to identify morpho-physiological traits of cotton that increase yield in agroecological cropping systems. Our approach combined a field experiment and crop simulation model(CSM) of CROPGRO-Cotton to evaluate the effects of genotype(G) × environment(E) × management(M) interactions on seed cotton yield(SCY).Results Cultivars Tamcot_camde and Okp768 and simulated ideotypes performed best in CA systems. Increased seed mass, large and thick leaves, and later maturity were identified as beneficial for yield enhancement in CA systems. Cultivars and ideotypes that combine these traits also resulted in better nitrogen and water use efficiencies in CA systems. Under different climate scenarios up to 2050, ideotypes designed could increase SCY in Benin.Conclusion A set of morpho-physiological traits associated with vegetative vigour is required to ensure a good SCY in agroecological cropping systems. These results provide scientific evidence and useful knowledge for breeders and research programmes on cropping systems focused on the adaptation of cotton to climate change.
基金funded by the Directorate General of Higher Education,Research,and Technology,Ministry of Education,Culture,Research,and Technology,Indonesia,which sponsored the research grant via the National Competitive Basic Research Scheme,No.027/E5/PG.02.00.PL/2024facilitated by IPB University(Bogor Agricultural University)through Research Contract No.22055/IT3.D10/PT.01.03/P/B/2024+1 种基金funded by Research Organization for Nanotechnology and Materials No.3/III.10/HK/2023National Research and Innovation Agency(BRIN),Indonesia.
文摘Furfurylation,a renowned chemical modification technique,uses furfuryl alcohol to enhance the properties of wood.This technology can be further refined by incorporating renewable tannins,which promote cross-linking with furfuryl alcohol.This study investigates the effects of furfurylation and tannin-modified furfurylation on the physical and mechanical properties of tropical Gmelina wood(Gmelina arborea Roxb.).Experiments involved impregnating Gmelina wood with aqueous solutions of furfuryl alcohol(FA)at 40%and 70%concentrations,as well as FA-tannin combinations(FA 40%-TA and FA 70%-TA),followed by polymerization at 103℃.The results demonstrated that both FA and FA-tannin treatments significantly improved the wood’s physical and mechanical properties.Notably,FA-tannin treatments achieved anti-swelling efficiency comparable to FA alone.However,the addition of tannins(FA 70%-TA)enhanced leaching resistance by up to 47%,contributing to a more environmentally sustainable modification process.Mechanically,the inclusion of tannins in FA 70%-TA slightly increased the modulus of elasticity(14%~8732 kg/cm^(2))and the modulus of rupture(9%~40.9 kg/cm^(2)).Furthermore,the tannin addition imparted a darker color to the modified wood,enhancing its aesthetic appeal.This study highlights the potential of tannin-modified furfurylation to advance wood modification technology,combining improved performance with environmental benefits.
