The Ca.LSU intron flanking a 129 bp cxon upstream and a fOO bp exondownstream was inserted into the lacZ gene on pRS426 to transform E.coli.Northern blot analysisand RT-PCR showed that splicing of Ca.LSU in E.coli is ...The Ca.LSU intron flanking a 129 bp cxon upstream and a fOO bp exondownstream was inserted into the lacZ gene on pRS426 to transform E.coli.Northern blot analysisand RT-PCR showed that splicing of Ca.LSU in E.coli is efficient upon inducible expression of theprecursor RNA,In contrast,co-lranscriptional self-splicing of the intron in vitro is much lessactive.Therefore,this E.coli splicing system can be used as a better model to investigate theeffect of the ribozyme inhibitors on Ca.LSUsplicing in living cell.We examined the effects of neomycin sulfatc and pentamidine on Ca.LSU splicing in E.coli,and found that these drugsdoes-dependently inhibit the intron splicing.However,heomycin is more potent than pentamidine inthis action.展开更多
Neuroendocrine tumors of the ovary tumors are little known and infrequent. They are made up of cells appearing in the endocrine and nervous systems. These tumors are rare, but most of them are very aggressive, especia...Neuroendocrine tumors of the ovary tumors are little known and infrequent. They are made up of cells appearing in the endocrine and nervous systems. These tumors are rare, but most of them are very aggressive, especially when they are diagnosed beyond FIGO Stage I. The distinction among the different subtypes is difficult and requires efficient techniques. Symptoms of neuroendocrine tumors are often nonspecific (redness in the face, diarrhea, asthma, abdominal pain and etc.,) and confused with more common diseases. Misdiagnosis is often asked: menopause or irritable bowel syndrome. Often the correct diagnosis is not made until years after the onset of symptoms. The relative rarity of these diseases and the lack of specificity of the symptoms make them often be diagnosed several years after onset. Fortunately, science has made significant advances in the diagnosis and treatment of neuroendocrine tumors. We report the case of a woman of 54 years, with abdominopelvic mass, in whom abdominal ultrasound revealed a mass avascular tissue Doppler taking the screen. The abdominopelvic CT showed a large cystic mass solido—abdominopelvic whose origin is difficult to determine. The patient underwent an exploratory laparotomy. Anapath the results of the right ovary were in favor of a well-differentiated neuroendocrine lesion.展开更多
While our current forecast for 2025 does not suggest a global recession,it points to a meaningful deceleration compared to historical benchmarks and now incorporates the rising geopolitical premium following the June ...While our current forecast for 2025 does not suggest a global recession,it points to a meaningful deceleration compared to historical benchmarks and now incorporates the rising geopolitical premium following the June Israel-Iran escalation.Global growth is projected at 2.6%,well below the 3.7%average registered between 2011 and 2019,the post-Global Financial Crisis(GFC)and pre-pandemic period.G3 growth remains muted:the US is expected to expand by just 0.3%on a Q4-over-Q4 basis in 2025,while the euro area and Japan are forecast to grow by 0.7%and 0.1%,respectively.展开更多
This article aims to outline the latest economic perspectives on the United States,the Euro Area,and Japan,collectively referred to as the G3 economies.As the post-International Monetary Fund(IMF)meetings landscape ha...This article aims to outline the latest economic perspectives on the United States,the Euro Area,and Japan,collectively referred to as the G3 economies.As the post-International Monetary Fund(IMF)meetings landscape has clarified,the economy now undergoes a new global equilibrium.Growth is neither collapsing nor accelerating,and policy easing is unfolding not in response to strength but as a reflection of fragility.These major economies are converging not toward synchronized expansion but toward their respective neutral interest rates.The result is a plateau marked by modest output,selective resilience,and deep structural fatigue.展开更多
The re-election of Donald Trump has led to immediate yet cautious reactions across global markets,as investors brace for potential shifts toward tax cuts,deregulation,and aggressive trade policies.U.S.equities initial...The re-election of Donald Trump has led to immediate yet cautious reactions across global markets,as investors brace for potential shifts toward tax cuts,deregulation,and aggressive trade policies.U.S.equities initially surged by 2-3%following the election results,while the U.S.dollar appreciated by 3%against a basket of currencies,reflecting expectations of tighter Fed stance owing to the inflationary impacts of higher tariffs,lower immigration flows to the United States,and loose fiscal policies.展开更多
Portfolio investment flow to China is a weathervane for China's financial health and global investor confidence.Chart 1 shows the capital flows via the Stock and Bond Connects in February-June.We studied the force...Portfolio investment flow to China is a weathervane for China's financial health and global investor confidence.Chart 1 shows the capital flows via the Stock and Bond Connects in February-June.We studied the forces that drive China's net bond and equity flows.It shows that relative economic growth,bond yields,RMB exchange rate,market volatility,and index inclusion explain about 50%of the flows(Chart 2).Many other factors,such as policy and geopolitical risk,which are hard to quantify,are also behind the ebb and flow of portfolio investments.展开更多
基金Supported by the National Natural Science Foundation of China(30170213)the Foundation of Wuhan University(0000028)
文摘The Ca.LSU intron flanking a 129 bp cxon upstream and a fOO bp exondownstream was inserted into the lacZ gene on pRS426 to transform E.coli.Northern blot analysisand RT-PCR showed that splicing of Ca.LSU in E.coli is efficient upon inducible expression of theprecursor RNA,In contrast,co-lranscriptional self-splicing of the intron in vitro is much lessactive.Therefore,this E.coli splicing system can be used as a better model to investigate theeffect of the ribozyme inhibitors on Ca.LSUsplicing in living cell.We examined the effects of neomycin sulfatc and pentamidine on Ca.LSU splicing in E.coli,and found that these drugsdoes-dependently inhibit the intron splicing.However,heomycin is more potent than pentamidine inthis action.
文摘Neuroendocrine tumors of the ovary tumors are little known and infrequent. They are made up of cells appearing in the endocrine and nervous systems. These tumors are rare, but most of them are very aggressive, especially when they are diagnosed beyond FIGO Stage I. The distinction among the different subtypes is difficult and requires efficient techniques. Symptoms of neuroendocrine tumors are often nonspecific (redness in the face, diarrhea, asthma, abdominal pain and etc.,) and confused with more common diseases. Misdiagnosis is often asked: menopause or irritable bowel syndrome. Often the correct diagnosis is not made until years after the onset of symptoms. The relative rarity of these diseases and the lack of specificity of the symptoms make them often be diagnosed several years after onset. Fortunately, science has made significant advances in the diagnosis and treatment of neuroendocrine tumors. We report the case of a woman of 54 years, with abdominopelvic mass, in whom abdominal ultrasound revealed a mass avascular tissue Doppler taking the screen. The abdominopelvic CT showed a large cystic mass solido—abdominopelvic whose origin is difficult to determine. The patient underwent an exploratory laparotomy. Anapath the results of the right ovary were in favor of a well-differentiated neuroendocrine lesion.
文摘While our current forecast for 2025 does not suggest a global recession,it points to a meaningful deceleration compared to historical benchmarks and now incorporates the rising geopolitical premium following the June Israel-Iran escalation.Global growth is projected at 2.6%,well below the 3.7%average registered between 2011 and 2019,the post-Global Financial Crisis(GFC)and pre-pandemic period.G3 growth remains muted:the US is expected to expand by just 0.3%on a Q4-over-Q4 basis in 2025,while the euro area and Japan are forecast to grow by 0.7%and 0.1%,respectively.
文摘This article aims to outline the latest economic perspectives on the United States,the Euro Area,and Japan,collectively referred to as the G3 economies.As the post-International Monetary Fund(IMF)meetings landscape has clarified,the economy now undergoes a new global equilibrium.Growth is neither collapsing nor accelerating,and policy easing is unfolding not in response to strength but as a reflection of fragility.These major economies are converging not toward synchronized expansion but toward their respective neutral interest rates.The result is a plateau marked by modest output,selective resilience,and deep structural fatigue.
文摘The re-election of Donald Trump has led to immediate yet cautious reactions across global markets,as investors brace for potential shifts toward tax cuts,deregulation,and aggressive trade policies.U.S.equities initially surged by 2-3%following the election results,while the U.S.dollar appreciated by 3%against a basket of currencies,reflecting expectations of tighter Fed stance owing to the inflationary impacts of higher tariffs,lower immigration flows to the United States,and loose fiscal policies.
文摘Portfolio investment flow to China is a weathervane for China's financial health and global investor confidence.Chart 1 shows the capital flows via the Stock and Bond Connects in February-June.We studied the forces that drive China's net bond and equity flows.It shows that relative economic growth,bond yields,RMB exchange rate,market volatility,and index inclusion explain about 50%of the flows(Chart 2).Many other factors,such as policy and geopolitical risk,which are hard to quantify,are also behind the ebb and flow of portfolio investments.
