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针对微量细胞样本进行细胞核和线粒体基因组同步快速分离的方法研究
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作者 胡胜 胡哲 汪方奎 《山东化工》 CAS 2024年第3期151-154,共4页
开发了一种针对微量细胞样本进行细胞核和线粒体基因组同步快速分离的方法。该方法首先利用体积分数1%非离子型表面活性剂NP-40能够选择性破坏细胞膜而维持细胞核完整性的特征,实现了细胞的轻柔裂解和细胞核完整性的维持;其次通过差速离... 开发了一种针对微量细胞样本进行细胞核和线粒体基因组同步快速分离的方法。该方法首先利用体积分数1%非离子型表面活性剂NP-40能够选择性破坏细胞膜而维持细胞核完整性的特征,实现了细胞的轻柔裂解和细胞核完整性的维持;其次通过差速离心,实现细胞核(核基因组)与线粒体基因组的同步分离。经过显微成像观察,核酸蛋白分析仪检测以及PCR分析,实现了从10^(2)到10^(6)个悬浮培养细胞中高质量线粒体基因组的分离纯化。本方法获取的线粒体基因组无核基因组污染,为后续开展线粒体群体遗传分析、线粒体疾病诊断提供了一种高效、快速、低成本的解决方案。 展开更多
关键词 线粒体分离纯化 微量细胞 NP40缓冲液
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川东地区汉族人群18个STR基因座遗传多态性 被引量:2
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作者 汪昌丽 范英南 +4 位作者 樊哲仁 姚伊人 杨帆 张国栋 喻永敏 《检验医学与临床》 CAS 2019年第1期1-4,共4页
目的应用直接扩增法批量检验并调查川东地区汉族人群DNA标本在18个STR基因座的遗传多态性。方法采用DNATyper15TMPlus试剂盒直接扩增和3730XL型遗传分析仪检测STR分型。随机抽取326例该地区汉族无关个体STR分型结果,并统计18个STR基因... 目的应用直接扩增法批量检验并调查川东地区汉族人群DNA标本在18个STR基因座的遗传多态性。方法采用DNATyper15TMPlus试剂盒直接扩增和3730XL型遗传分析仪检测STR分型。随机抽取326例该地区汉族无关个体STR分型结果,并统计18个STR基因座的等位基因分布频率及其他群体遗传学参数。结果 18个STR基因座基因型的分布符合Hardy-Weinberg平衡(P>0.05),川东地区汉族人群18个STR基因座的杂合度、匹配概率、多态信息含量、个体识别概率、非父排除概率的范围分别为0.604~0.896、0.016~0.219、0.545~0.908、0.781~0.984、0.295~0.788。结论使用直接扩增法批量检验DNA数据库标本的效率高、质量好;18个STR基因座的联合应用能获得较好的系统效能,为该地区汉族人群的遗传多态性研究及应用提供数据支持。 展开更多
关键词 直接扩增 STR基因座 遗传多态性 川东地区 汉族
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Enhancement of the Photodynamic Therapy Effect on Streptococcus Mutans Biofilm 被引量:1
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作者 Vitor Hugo Panhoca Mariana Carreira Geralde +3 位作者 Thaila Quatrini Correa Mariana Torres Carvalho Clovis Wesley Oliveira Souza Vanderlei Salvador Bagnato 《Journal of Physical Science and Application》 2014年第2期107-114,共8页
Biofilm is a community of bacteria,less susceptible to traditions treatments.Although photodynamic therapy(PDT)is a very effective way to microorganism inactivation,in biofilm it is not as efficient as it is in plankt... Biofilm is a community of bacteria,less susceptible to traditions treatments.Although photodynamic therapy(PDT)is a very effective way to microorganism inactivation,in biofilm it is not as efficient as it is in planktonic bacteria cultures.The increment of an element to increase the effectiveness of PDT was our aim.Therefore,this in vitro study evaluates the susceptibility ofa biofilm formed by Streptococcus mutans on metallic surface of orthodontic accessories under the application of PDT with a surfactant.Samples obtained from blades of orthodontic bands(NiCr),where used as adhesion surface for the biofilm.They were treated with 1 mg/ml of curcumin,with 0.1%of sodium dodecyl sulfate and exposed to 30 J/cm^2 of light(455 nm).Eight experimental groups were studied,including the positive and negative controls.The results show that the group with PDT and surfactant had a significant decrease(p〈0.001)in viability.In this case,the reduction observed was of 5.6 log10(CFU/ml)in comparison to the control group.We have shown that,even though the biofilm is very tough and complex structure,we are able to promote almost the complete inactivation ofS.mutans in systems similar to an orthodontic treated patient's mouth. 展开更多
关键词 Photodynamic therapy light-induced damage oral biofilm streptococcus mutans CURCUMIN SURFACTANT
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DC Plasma Technology Applied to Powder Metallurgy: an Overview 被引量:1
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作者 A. N. KLEIN R. P. CARDOSO +7 位作者 H. C. PAVANATI C. BINDER A. M. MALISKA G. HAMMES D. FUSAO A. SEEBER S. F. BRUNATTO J. L. R. MUZART 《Plasma Science and Technology》 SCIE EI CAS CSCD 2013年第1期70-81,共12页
DC plasma is a very promising technology for processing different materials, and is becoming especially interesting when low environmental impact and high-performance treatments are needed. Some of the intrinsic chara... DC plasma is a very promising technology for processing different materials, and is becoming especially interesting when low environmental impact and high-performance treatments are needed. Some of the intrinsic characteristics of DC plasma technology, which make it suitable for powder metallurgy (PM) and powder injection molding (PIM) parts production, are low- pressure processing and plasma environment high reactivity. Moreover it can be considered as a highly competitive green technology. In this work, an overview of some of the important DC plasma techniques applied to PM and PIM parts processing is presented. Emphasis is given to the descriptions of the main characteristics and the technique potentials of plasma-assisted nitriding, plasma-assisted thermal debinding, plasma-assisted sintering, and simultaneously plasma-assisted sintering and surface alloying. The aspects presented and discussed in this paper indicate that DC plasma processes are promising and competitive techniques for PM and PIM parts processing. 展开更多
关键词 powder metallurgy DC glow discharge plasma nitriding plasma sintering plasma-assisted thermal debinding surface alloying
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Using a Health Level 7 Interoperability Bus to Support Legacy Systems in the Health Domain
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作者 Rafael Andrade Aldo von Wangenheim +1 位作者 Alexandre Savaris Karine Petry 《E-Health Telecommunication Systems and Networks》 2013年第4期72-80,共9页
In order to reduce the effort in the integration and actualization of heterogeneous healthcare legacy systems that should share a common database, we propose the creation of an interoperability bus using the HL7 stand... In order to reduce the effort in the integration and actualization of heterogeneous healthcare legacy systems that should share a common database, we propose the creation of an interoperability bus using the HL7 standard—the HL7Middleware. This interoperability bus is an intermediate layer responsible for the communication between a database, health information systems and medical equipment, called HL7Server. Connected systems use the HL7 messaging semantics to store and retrieve data from the database. We validate our approach with respect to two different criteria: performance and integration costs. Benchmark tests were executed with and without the use of HL7Middleware and with different network bandwidths. These results demonstrated that the performance of the interoperability bus is higher when compared to traditional database access for larger volumes of data and when the bandwidth of the user is considerably lower than the bandwidth of the connection between HL7Server and database. The overall development and deployment cost was considered low and the reusability degree of wrapper code was considered high, thus suggesting a progressive reduction of the integration costs of additional services and subsystems of an organization. 展开更多
关键词 MIDDLEWARE Healthcare INTEROPERABILITY BUS LEGACY System HL7 TELEMEDICINE
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Red-emitting CaWO4:Eu^(3+),Tm^(3+)phosphor for solid-state lighting:Luminescent properties and morphology evolution
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作者 L.X.Lovisa I.M.P.da Silva +5 位作者 A.A.G.Santiago M.Siu Li E.Longo C.A.Paskocimas M.R.D.Bomio F.V.Motta 《Journal of Rare Earths》 SCIE EI CAS CSCD 2022年第2期226-233,I0002,共9页
CaWO_(4):xEu^(3+),yTm^(3+)crystals were obtained by facile synthesis at low temperature by the microwaveassisted hydrothermal method(MAH).The phase formation,morphology,luminescent properties and ene rgy transfer were... CaWO_(4):xEu^(3+),yTm^(3+)crystals were obtained by facile synthesis at low temperature by the microwaveassisted hydrothermal method(MAH).The phase formation,morphology,luminescent properties and ene rgy transfer were investigated.The X-ray diffraction(XRD)re sults show the formation of a scheelitelike tetragonal structure without the presence of secondary phases.The growth mechanism of hierarchical micro structures based on self-assembly and Ostwald-ripening processes was evaluated,obtaining different types of morphologies.The luminescence spectra of CaWO_(4):Eu^(3+),Tm^(3+)at 325 nm excitation show the predominance of red emission at the 5 D0→7 F2(Eu^(3+))transition at 624 nm.This feature signals dominant behavior of the electric dipole type.The presence of Tm^(3+)is notably evident in the absorption spectra by the related excitation transitions:3 H6→1 G4,3 H6→3 F3 and 3 H6→3 H4.Color parameters are discussed to characterize CaWO_(4):Eu^(3+),Tm^(3+)emission.The study of the emission spectrum as a function of the concentration of Eu^(3+)(x mol%)and Tm^(3+)(y mol%)indicates that the CaWO_(4):Eu^(3+),Tm^(3+)phosphors show stronger red emission intensity and exhibit the CIE value of x=0.63 and y=0.35.The photoluminescence results show 97%high color purity for CaWO_(4):4 mol%Eu^(3+),a high CRI(92%)and a low CCT of 1085 K.These results demonstrate that the CaWO_(4):Eu^(3+),Tm^(3+)red phosphors are promising as color converters for application in white light-emitting diodes and display devices. 展开更多
关键词 CaWO_(4):Eu^(3+) Tm^(3+)microwave-assisted hydrothermal method Photoluminescence Red phosphor Rare earths
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