Triadimefon(TDM)and cyproconazole(CPZ)are two triazoles widely used as fungicides.Several azoles were synthe-sised starting from commercial TDM and CPZ.The compounds were evaluated against phytopathogenic filamentous ...Triadimefon(TDM)and cyproconazole(CPZ)are two triazoles widely used as fungicides.Several azoles were synthe-sised starting from commercial TDM and CPZ.The compounds were evaluated against phytopathogenic filamentous fungi,including Aspergillus fumigatus(AF),A.niger(AN),A.ustus(AU),A.japonicus(AJ),A.terreus(AT),Fusarium oxyspo-rum and Botrytis cinerea isolated from grapevine in the province of San Juan,Argentina.Three of the synthesised compounds(1-(Biphenyl-4-yloxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1-yl)butan-2-one,1;2-(Biphenyl-4-yl)-3-cyclopropyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol,3;3-Cyclopropyl-2-(4’-fluorobiphenyl-4-yl)-1-(1H-1,2,4-triazol1-yl)butan-2-ol,4)presented remarkable in vitro fungicidal properties,with better effects than TDM and CPZ on some of the target fungi.Cytotoxicity was assessed using human lung fibroblasts MRC5.Derivative 1,with IC50 values of 389.4μM,was less toxic towards MRC-5 human lung fibroblasts than commercial TDM(248.5μM)and CPZ(267.4μM).Docking analysis and molecular dynamics simulations suggest that the compounds present the same interaction in the binding pocket of the CYP51B enzyme and with the same amino acids as CPZ.The derivatives investigated could be considered broad-spectrum but with some selectivity towards imperfect fungi.展开更多
High-intensity ultrasound(HIU)is a sustainable technology that modifies the techno-functional properties of foods,preserving their nutritional quality and aligning with current food trends.HIU was applied to a cowpea ...High-intensity ultrasound(HIU)is a sustainable technology that modifies the techno-functional properties of foods,preserving their nutritional quality and aligning with current food trends.HIU was applied to a cowpea flour aqueous suspension(10%w/v)to optimize the amplitude and time conditions through a response surface methodology,aiming to maximize the techno-functional properties of flours.Compositional,structural,morphological and thermal studies of two optimums C-US/87–15(87% amplitude/15 min HIU),C-US/39–52(39%amplitude/52 min HIU)and the untreated flour(NC)were carried out.HIU decreased the bulk density and increased the water and oil retention capacity,solubility and swelling power of the flour.HIU did not change the macronutrient content or in starch granule morphology;however,it significantly increased the amylose content due to the cleavage ofα(1→6)bonds in the amylopectin branches,and increased the total dietary fiber(16.11±0.04 g/100gflour to 18.3±0.4 g/100gflour and 17.0±0.4 g/100gflour),with the conversion of insoluble to soluble fiber.HIU unified the particle size(trimodal to bimodal distribution),increased the starch crystalline structures(FT-IR ratio 1022/995)and the peak viscosity from 1.7755±0.0007 Pa.s to 1.90±0.07 Pa.s and 1.91±0.05 Pa.s.Gelatinization enthalpy reflected a greater molecular order,confirming the increase in crystalline structures as a result of HIU.This research demonstrates that HIU modifies the techno-functional properties of cowpea flour,expanding the possibilities for the production of plant-based foods using legume flours with better technological qualities with a gluten-free approach.展开更多
This study explored the potential of black rice phenolics(BRP)in managing type 2 diabetes mellitus(T2DM)by examining their effects on oxidative stress,metabolic pathways,and gut microbiota.The results indicated that B...This study explored the potential of black rice phenolics(BRP)in managing type 2 diabetes mellitus(T2DM)by examining their effects on oxidative stress,metabolic pathways,and gut microbiota.The results indicated that BRP significantly alleviated diabetic symptoms,lowered fasting blood glucose and Hemoglobin A1c(HbA1c)levels,and enhanced glucose tolerance in T2DM rats.Additionally,BRP reduced serum triglyceride(TG)and total cholesterol(TC)levels,improved kidney and liver function,and decreased oxidative stress in plasma and liver.BRP also increased gut microbiota diversity,specifically promoting the growth of Verrucomicrobia and Akkermansia.Furthermore,BRP modulated serum,cecum,and colon metabolites,particularly those linked to tryptophan,vitamin,and lipid metabolism,offering insights into its glucose-lowering mechanisms.These findings suggest that BRP exerts anti-diabetic effects by modulating gut microbiota and metabolic pathways,thus improving glucose metabolism.This study provides a basis for developing BRP-based functional foods targeting diabetes management.展开更多
Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PE...Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PEO)lower in pulegone/MENT(menthone+isomenthone)ratio combined with butylated hydroxytoluene(BHT).PEO was performed by gas chromatography with mass spectrometry(GC-MS).The thermal stability of PEO was determined at 60℃ for 28 days and analysed by GC-MS with SPME.Free Radical Scavening Activity(FRSA-DPPH test)and Total Phenol Content(TPC-analysed with Folin-cicolteau reagent)determined indirect AA.Direct AA was performed with accelerated oxidation of sunflower oil for 28 days at 60℃.Concentrations of 0.02,0.01 and 0.005%w/w(1,2 and 3)were used for the antioxidant BHT(B1,B2 and B3),PEO(P1,P2 and P3)and combinations.Chemical indicators(Conjugated Dienes,Peroxide Value and Anisidine Value),complete oxidation(Total Oxidation Value and Integral Oxidation Value)and volatiles(2-heptenal(Z)and 2,4-Decadienal(E,E))were determined.The pulegone/MENT ratio was 0.59 but the combinations B2P2 and B2P1 allow the use of lower concentration of BHT with higher efficiency.The low pulegone/MENT PEO can be combined with BHT to delay oxidation.展开更多
The potential of camellia oil(CO)to alleviate type 2 diabetes mellitus(T2DM)by modulating gut microbiota and metabolites was investigated.CO significantly reduced the fasting blood glucose(FBG)levels,improved glucose ...The potential of camellia oil(CO)to alleviate type 2 diabetes mellitus(T2DM)by modulating gut microbiota and metabolites was investigated.CO significantly reduced the fasting blood glucose(FBG)levels,improved glucose tolerance,and alleviated symptoms of polydipsia and polyphagia in db/db mice.Additionally,CO intervention increased gut microbiota richness and diversity and decreased the Firmicutes/Bacteroidetes(F/B)ratio.CO also increased beneficial bacteria abundance,including Clostridium_sensu_stricto_1,Christensenellaceae_R-7_group,and Proteiniclasticum,which correlated with improved glucose tolerance.Conversely,CO decreased pathogenic bacteria abundance,including Rodentibacter and Vibrio,which were positively associated with FBG levels.Metabolomic analysis further revealed that CO increased feces lysophosphatidyl lipid LPG 16:1 and bile acid β-muricholic acid levels,both negatively associated with FBG levels.These findings underscore CO’s potential as a functional oil with hypoglycemic effects,highlighting its therapeutic value in managing T2DM via gut microbiota and metabolic pathway modulation.展开更多
As natural bioactive compounds with recognized antioxidant power,polyphenols may impact on several diseases associated with oxidative stress.In this study,defatted sesame flour(DSF)was investigated as a potential sour...As natural bioactive compounds with recognized antioxidant power,polyphenols may impact on several diseases associated with oxidative stress.In this study,defatted sesame flour(DSF)was investigated as a potential source of dietary polyphenols and a functional ingredient.Additionally,the impact of manufacture and digestion processes on the biological efficacy of the resulting extracts in the HepG2 cell line was explored.For this purpose,polyphenolic extracts were obtained from three sources:DSF,a control sweet cookie formulation and 10% DSF-enriched cookies.In addition,extracts of potentially bioaccessible polyphenols from in vitro digested cookies were collected.Cells were incubated with all these extracts and then injured with H_(2)O_(2).Intracellular oxidative state and viability,antioxidant enzymes activities,glutathione(GSH)content,and oxidation of proteins and lipids were analyzed.The results showed DSF pro-oxidant actions on the cellular redox state,which hormetically enhanced the antioxidant response.Similarly,biological activity of DSF enriched cookies was found to persist after manufacture and digestion,promoting lipid peroxidation alleviation and GSH replenishment.By promoting an antioxidant response in cells,DSF may be considered for functional incorporation,offering preventive benefits and protective action to counterbalance oxidative damage.This study contributes to highlighting the benefits of DFS validating its final biological effects in a cellular model.展开更多
Refined rice flour and wholegrain quinoa and buckwheat flours were used to prepare gluten-free sourdoughs.Two autochthonous LAB strains were isolated,one from quinoa (Limosilactobacillus fermentum Q3) and the other fr...Refined rice flour and wholegrain quinoa and buckwheat flours were used to prepare gluten-free sourdoughs.Two autochthonous LAB strains were isolated,one from quinoa (Limosilactobacillus fermentum Q3) and the other from buckwheat (Lim.fermentum T5).Lactiplantibacillus plantarum ATCC8014 was used as a reference starter.Two fermentation processes were evaluated,a single-step process (30℃,24 h) named SD1,and a daily refreshment process (backslopping for 10 days) named SD2.Sourdoughs were freeze-dried prior to analyses.Pasting properties,pentosan and polyphenol contents,and antioxidant activity were analysed.Flour properties were significantly modified by fermentation.Polyphenol extractability increased during fermentation,which resulted in a higher antioxidant capacity,mainly through a reducing mechanism.Q3 and T5 strains produced the most remarkable modifications of flour properties.Differences were found between both fermentation processes,mainly as a result of the development of different microbiota.The single-step fermentation (SD1) showed to be a simple and appropriate method for improving flour properties.展开更多
Lipid oxidation in foods is delayed through the use of antioxidants.The aim was to determine the oxidative stability of high oleic peanut oil and the effect of adding oregano and hop essential oils,with phenolic and n...Lipid oxidation in foods is delayed through the use of antioxidants.The aim was to determine the oxidative stability of high oleic peanut oil and the effect of adding oregano and hop essential oils,with phenolic and no-phenolic terpenes,under 150℃.The peanut oil was enriched with oregano and hop essential oils(0.02%).These treatments were compared with sunflower oil,high oleic peanut oil,and high oleic peanut oil with BHT(0.02%).Treatments were exposed at 150℃ for 8 h.Peroxide value(PV),conjugated dienes,and volatile oxidation compounds were measured.High oleic peanut oil was more stable than sunflower oil.Peanut oil obtained 61.9%less PV than sunflower oil.Oregano and hop essential oils protected the peanut oil;the peroxide values were 14.9±0.4 and 14.6±0.2 meq O2 kg^(-1) at 8 h,respectively.Both“Chain Breaking Antioxidant”And“Termination Enhancing Antioxidant”had similar antioxidant effects.展开更多
文摘Triadimefon(TDM)and cyproconazole(CPZ)are two triazoles widely used as fungicides.Several azoles were synthe-sised starting from commercial TDM and CPZ.The compounds were evaluated against phytopathogenic filamentous fungi,including Aspergillus fumigatus(AF),A.niger(AN),A.ustus(AU),A.japonicus(AJ),A.terreus(AT),Fusarium oxyspo-rum and Botrytis cinerea isolated from grapevine in the province of San Juan,Argentina.Three of the synthesised compounds(1-(Biphenyl-4-yloxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1-yl)butan-2-one,1;2-(Biphenyl-4-yl)-3-cyclopropyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol,3;3-Cyclopropyl-2-(4’-fluorobiphenyl-4-yl)-1-(1H-1,2,4-triazol1-yl)butan-2-ol,4)presented remarkable in vitro fungicidal properties,with better effects than TDM and CPZ on some of the target fungi.Cytotoxicity was assessed using human lung fibroblasts MRC5.Derivative 1,with IC50 values of 389.4μM,was less toxic towards MRC-5 human lung fibroblasts than commercial TDM(248.5μM)and CPZ(267.4μM).Docking analysis and molecular dynamics simulations suggest that the compounds present the same interaction in the binding pocket of the CYP51B enzyme and with the same amino acids as CPZ.The derivatives investigated could be considered broad-spectrum but with some selectivity towards imperfect fungi.
基金funded by Agencia de Investigacion,Desarrollo e Innovacion de Argentina(PICT-2020-01944)Consejo Nacional de Investigaciones Científicas y Tecnicas(PIP 11220200102900CO and PIBAA 28720210100948CO)Secretaría General de Ciencia y Tecnica of Universidad Nacional del Nordeste(PI,2022F010).
文摘High-intensity ultrasound(HIU)is a sustainable technology that modifies the techno-functional properties of foods,preserving their nutritional quality and aligning with current food trends.HIU was applied to a cowpea flour aqueous suspension(10%w/v)to optimize the amplitude and time conditions through a response surface methodology,aiming to maximize the techno-functional properties of flours.Compositional,structural,morphological and thermal studies of two optimums C-US/87–15(87% amplitude/15 min HIU),C-US/39–52(39%amplitude/52 min HIU)and the untreated flour(NC)were carried out.HIU decreased the bulk density and increased the water and oil retention capacity,solubility and swelling power of the flour.HIU did not change the macronutrient content or in starch granule morphology;however,it significantly increased the amylose content due to the cleavage ofα(1→6)bonds in the amylopectin branches,and increased the total dietary fiber(16.11±0.04 g/100gflour to 18.3±0.4 g/100gflour and 17.0±0.4 g/100gflour),with the conversion of insoluble to soluble fiber.HIU unified the particle size(trimodal to bimodal distribution),increased the starch crystalline structures(FT-IR ratio 1022/995)and the peak viscosity from 1.7755±0.0007 Pa.s to 1.90±0.07 Pa.s and 1.91±0.05 Pa.s.Gelatinization enthalpy reflected a greater molecular order,confirming the increase in crystalline structures as a result of HIU.This research demonstrates that HIU modifies the techno-functional properties of cowpea flour,expanding the possibilities for the production of plant-based foods using legume flours with better technological qualities with a gluten-free approach.
基金National Natural Science Foundation of China(32260557)Jiangxi Provincial Natural Science Foundation(20224BAB215049)+1 种基金the High-level Talents’Scientific Research Foundation of Gannan Medical University(QD202123)Science and Technology Plan Project of Ganzhou(2022DSYS9831).
文摘This study explored the potential of black rice phenolics(BRP)in managing type 2 diabetes mellitus(T2DM)by examining their effects on oxidative stress,metabolic pathways,and gut microbiota.The results indicated that BRP significantly alleviated diabetic symptoms,lowered fasting blood glucose and Hemoglobin A1c(HbA1c)levels,and enhanced glucose tolerance in T2DM rats.Additionally,BRP reduced serum triglyceride(TG)and total cholesterol(TC)levels,improved kidney and liver function,and decreased oxidative stress in plasma and liver.BRP also increased gut microbiota diversity,specifically promoting the growth of Verrucomicrobia and Akkermansia.Furthermore,BRP modulated serum,cecum,and colon metabolites,particularly those linked to tryptophan,vitamin,and lipid metabolism,offering insights into its glucose-lowering mechanisms.These findings suggest that BRP exerts anti-diabetic effects by modulating gut microbiota and metabolic pathways,thus improving glucose metabolism.This study provides a basis for developing BRP-based functional foods targeting diabetes management.
文摘Minthostachys mollis(Benth)Griseb.is a variety of‘peperina’with high pulegone+methone content but with a higher menthone content.The objective was to evaluate the antioxidant activity(AA)of peperina essential oil(PEO)lower in pulegone/MENT(menthone+isomenthone)ratio combined with butylated hydroxytoluene(BHT).PEO was performed by gas chromatography with mass spectrometry(GC-MS).The thermal stability of PEO was determined at 60℃ for 28 days and analysed by GC-MS with SPME.Free Radical Scavening Activity(FRSA-DPPH test)and Total Phenol Content(TPC-analysed with Folin-cicolteau reagent)determined indirect AA.Direct AA was performed with accelerated oxidation of sunflower oil for 28 days at 60℃.Concentrations of 0.02,0.01 and 0.005%w/w(1,2 and 3)were used for the antioxidant BHT(B1,B2 and B3),PEO(P1,P2 and P3)and combinations.Chemical indicators(Conjugated Dienes,Peroxide Value and Anisidine Value),complete oxidation(Total Oxidation Value and Integral Oxidation Value)and volatiles(2-heptenal(Z)and 2,4-Decadienal(E,E))were determined.The pulegone/MENT ratio was 0.59 but the combinations B2P2 and B2P1 allow the use of lower concentration of BHT with higher efficiency.The low pulegone/MENT PEO can be combined with BHT to delay oxidation.
基金supported by Scientific Research Projects Supported by Ganzhou General Inspection and Testing Institute(HX202106)High-level Talents'Scientific Research Foundation of Gannan Medical University(QD202123)+2 种基金Jiangxi Provincial Natural Science Foundation(20224BAB215049)National Natural Science Foundation of China(32260557)Science and Technology Plan Project of Ganzhou(2022DSYS9831).
文摘The potential of camellia oil(CO)to alleviate type 2 diabetes mellitus(T2DM)by modulating gut microbiota and metabolites was investigated.CO significantly reduced the fasting blood glucose(FBG)levels,improved glucose tolerance,and alleviated symptoms of polydipsia and polyphagia in db/db mice.Additionally,CO intervention increased gut microbiota richness and diversity and decreased the Firmicutes/Bacteroidetes(F/B)ratio.CO also increased beneficial bacteria abundance,including Clostridium_sensu_stricto_1,Christensenellaceae_R-7_group,and Proteiniclasticum,which correlated with improved glucose tolerance.Conversely,CO decreased pathogenic bacteria abundance,including Rodentibacter and Vibrio,which were positively associated with FBG levels.Metabolomic analysis further revealed that CO increased feces lysophosphatidyl lipid LPG 16:1 and bile acid β-muricholic acid levels,both negatively associated with FBG levels.These findings underscore CO’s potential as a functional oil with hypoglycemic effects,highlighting its therapeutic value in managing T2DM via gut microbiota and metabolic pathway modulation.
基金mainly supported by PICT 2017-1637,PICT 2020-1588,SECyT 2018-2020,PIP 202111220200101422COAgustin Lucini Mas hold a doctoral fellowship and María Eugenia Sabatino,Verónica Mary,Marcela Martinez,Daniel Alberto Wunderlin,and Maria Verónica Baroni are researchers from CONICET(National Council of Scientific and Technical Research,Argentina).
文摘As natural bioactive compounds with recognized antioxidant power,polyphenols may impact on several diseases associated with oxidative stress.In this study,defatted sesame flour(DSF)was investigated as a potential source of dietary polyphenols and a functional ingredient.Additionally,the impact of manufacture and digestion processes on the biological efficacy of the resulting extracts in the HepG2 cell line was explored.For this purpose,polyphenolic extracts were obtained from three sources:DSF,a control sweet cookie formulation and 10% DSF-enriched cookies.In addition,extracts of potentially bioaccessible polyphenols from in vitro digested cookies were collected.Cells were incubated with all these extracts and then injured with H_(2)O_(2).Intracellular oxidative state and viability,antioxidant enzymes activities,glutathione(GSH)content,and oxidation of proteins and lipids were analyzed.The results showed DSF pro-oxidant actions on the cellular redox state,which hormetically enhanced the antioxidant response.Similarly,biological activity of DSF enriched cookies was found to persist after manufacture and digestion,promoting lipid peroxidation alleviation and GSH replenishment.By promoting an antioxidant response in cells,DSF may be considered for functional incorporation,offering preventive benefits and protective action to counterbalance oxidative damage.This study contributes to highlighting the benefits of DFS validating its final biological effects in a cellular model.
基金supported by Consejo Nacional de Investigaciones Científicas y T´ecnicas and the Agencia Nacional de Promoci´on Científica y Tecnol´ogica(PICT 2015-3803)of Argentina.
文摘Refined rice flour and wholegrain quinoa and buckwheat flours were used to prepare gluten-free sourdoughs.Two autochthonous LAB strains were isolated,one from quinoa (Limosilactobacillus fermentum Q3) and the other from buckwheat (Lim.fermentum T5).Lactiplantibacillus plantarum ATCC8014 was used as a reference starter.Two fermentation processes were evaluated,a single-step process (30℃,24 h) named SD1,and a daily refreshment process (backslopping for 10 days) named SD2.Sourdoughs were freeze-dried prior to analyses.Pasting properties,pentosan and polyphenol contents,and antioxidant activity were analysed.Flour properties were significantly modified by fermentation.Polyphenol extractability increased during fermentation,which resulted in a higher antioxidant capacity,mainly through a reducing mechanism.Q3 and T5 strains produced the most remarkable modifications of flour properties.Differences were found between both fermentation processes,mainly as a result of the development of different microbiota.The single-step fermentation (SD1) showed to be a simple and appropriate method for improving flour properties.
文摘Lipid oxidation in foods is delayed through the use of antioxidants.The aim was to determine the oxidative stability of high oleic peanut oil and the effect of adding oregano and hop essential oils,with phenolic and no-phenolic terpenes,under 150℃.The peanut oil was enriched with oregano and hop essential oils(0.02%).These treatments were compared with sunflower oil,high oleic peanut oil,and high oleic peanut oil with BHT(0.02%).Treatments were exposed at 150℃ for 8 h.Peroxide value(PV),conjugated dienes,and volatile oxidation compounds were measured.High oleic peanut oil was more stable than sunflower oil.Peanut oil obtained 61.9%less PV than sunflower oil.Oregano and hop essential oils protected the peanut oil;the peroxide values were 14.9±0.4 and 14.6±0.2 meq O2 kg^(-1) at 8 h,respectively.Both“Chain Breaking Antioxidant”And“Termination Enhancing Antioxidant”had similar antioxidant effects.
