Yerba mate (Ilex paraguariensis) is an indigenous crop which is highly consumed as an infusion in the South American subtropical forest. It has a high concentration of antioxidant substances, providing health benefits...Yerba mate (Ilex paraguariensis) is an indigenous crop which is highly consumed as an infusion in the South American subtropical forest. It has a high concentration of antioxidant substances, providing health benefits and helping to prevent diseases. The objectives of this work were to characterize an aqueous yerba mate extract by spectrophotometric and chromatographic (HPLC) methods and to study the effect of the freeze-drying process on the polyphenols profile and antioxidant activity, determined by a novel method. An aqueous extract was obtained and lyophilized to obtain a yerba mate powder with antioxidant properties. The extracts showed a high polyphenol content, determined by Folin-Ciocalteau and HPLC, and a high antioxidant activity towards the DPPH· radical and after the recently developed method of photochemiluminescence. A linear correlation was found between Folin-Ciocalteau and DPPH methods for lyophilized samples. HPLC analysis allowed determining antioxidant components like rutin, caffeine and chlorogenic acid. Lyophilization caused a decrease in total polyphenol content and antiradical activity of the extracts and this fact was mainly attributed to changes in the chlorogenic acid related compounds and rutin structures, after their photochemiluminescence data. The photoluminiscent method proves to be an advantageous approach for antioxidant capacity determination.展开更多
Purpose:This study was aimed to analyze the mediation role of cardiorespiratory fitness(CRF)on the association between fatness and cardiometabolic risk scores(CMRs)in European adolescents.Methods:A cross-sectional stu...Purpose:This study was aimed to analyze the mediation role of cardiorespiratory fitness(CRF)on the association between fatness and cardiometabolic risk scores(CMRs)in European adolescents.Methods:A cross-sectional study was conducted in adolescents(n=525;46%boys;14.1±1.1 years old,mean ±SD)from 10 European cities involved in the Healthy Lifestyle in Europe by Nutrition in Adolescence study.CRF was measured by means of the shuttle run test,while fatness measures included body mass index(BMI),waist to height ratio,and fat mass index estimated from skinfold thicknesses.A clustered CMRs was computed by summing the standardized values of homeostasis model assessment,systolic blood pressure,triglycerides,total cholesterol/highdensity lipoprotein cholesterol ratio,and leptin.Results:Linear regression models indicated that CRF acted as an important and partial mediator in the association between fatness and CMRs in 1217-year-old adolescents(for BMI:coefficients of the indirect role b=0.058(95%confidence interval(95%CI):0.023-0.101),Sobel test z=3.11(10.0%mediation);for waist to height ratio:b=4.279(95%CI:2.242-7.059),z=3.86(11.5%mediation);and for fat mass index:b=0.060(95%CI:0.020-0.106),z=2.85(9.4%mediation);all p<0.01).Conclusion:In adolescents,the association between fatness and CMRs could be partially decreased with improvements to fitness levels;therefore,CRF contribution both in the clinical field and public health could be important to consider and promote in adolescents independently of their fatness levels.展开更多
In this work, a grape phenolic extract obtained by methanol extraction has been demonstrated to be effective in inhibiting the growth of different strains and species of Campylobacter, one of the most important bacter...In this work, a grape phenolic extract obtained by methanol extraction has been demonstrated to be effective in inhibiting the growth of different strains and species of Campylobacter, one of the most important bacterial foodborne pathogens causing gastroenteritis worldwide. Noteworthily, it was particularly effective against several strains presenting multiple antibiotic resistances. In all cases, the minimum inhibitory concentration (MIC) was lower than 300 mg GAE/L, being of 60 mg GAE/L for one of the most resistant strains (C. coli LP2), while the others were between 120 mg GAE/L and 180 mg GAE/L. The analytical study of the main phenolic compounds in the grape extract revealed that it was mainly constituted by catechins (85.7%) and phenolic acids (13.7%). However, experiments developed using pure standards demonstrate that phenolic acids (such as gallic, p-hidroxibenzoic, vanillic, and homovanillic acids) were the most active, provoking a Campylobacter growth decrease between 6.7 and 7.6 log, while epicatechin was the only catechin with activity as pure compound (1 log of growth decrease).展开更多
This study investigated the ability of Lactiplantibacillus paraplantarum CRL2051 and Lactiplantibacillus plantarum CRL2030,two lactic acid bacteria isolated from fruits,to ferment pomegranate juice to produce beverage...This study investigated the ability of Lactiplantibacillus paraplantarum CRL2051 and Lactiplantibacillus plantarum CRL2030,two lactic acid bacteria isolated from fruits,to ferment pomegranate juice to produce beverages with extended shelf life and functional properties.Lactic acid bacteria consumed glucose and fructose as carbon sources,producing mainly lactic acid.Both strains were initially grown in juice at 30℃ for 48 h,then refrigerated at 4℃ for 28 days.During fermentation and cold storage,the total phenolic content and antioxidant capacity were conserved in fermented pomegranate juices.The functionality studies of the fermented beverages administered to C57BL/6 mice fed a high-fat diet were evaluated over 6 weeks.Pomegranate juice supplementation led to a marked improvement in glucose and triglycerides serum profile,which decreased around 40%and up to 48%,respectively,and counteracted fat deposition and body weight gain.The high-fat diet treatment significantly incremented the aspartate aminotransferase activity in the liver,whereas fermented pomegranate juice consumption slightly reduced it.Liver histological changes associated with a high-fat diet were significantly reverted in juice-treated animals.Our findings evidenced the biological benefits of a novel functional beverage obtained by lactic fermentation of pomegranate juice;this functional drink may protect against weight gain,liver damage,and dyslipidemia induced by a high-fat diet consumption.展开更多
文摘Yerba mate (Ilex paraguariensis) is an indigenous crop which is highly consumed as an infusion in the South American subtropical forest. It has a high concentration of antioxidant substances, providing health benefits and helping to prevent diseases. The objectives of this work were to characterize an aqueous yerba mate extract by spectrophotometric and chromatographic (HPLC) methods and to study the effect of the freeze-drying process on the polyphenols profile and antioxidant activity, determined by a novel method. An aqueous extract was obtained and lyophilized to obtain a yerba mate powder with antioxidant properties. The extracts showed a high polyphenol content, determined by Folin-Ciocalteau and HPLC, and a high antioxidant activity towards the DPPH· radical and after the recently developed method of photochemiluminescence. A linear correlation was found between Folin-Ciocalteau and DPPH methods for lyophilized samples. HPLC analysis allowed determining antioxidant components like rutin, caffeine and chlorogenic acid. Lyophilization caused a decrease in total polyphenol content and antiradical activity of the extracts and this fact was mainly attributed to changes in the chlorogenic acid related compounds and rutin structures, after their photochemiluminescence data. The photoluminiscent method proves to be an advantageous approach for antioxidant capacity determination.
文摘Purpose:This study was aimed to analyze the mediation role of cardiorespiratory fitness(CRF)on the association between fatness and cardiometabolic risk scores(CMRs)in European adolescents.Methods:A cross-sectional study was conducted in adolescents(n=525;46%boys;14.1±1.1 years old,mean ±SD)from 10 European cities involved in the Healthy Lifestyle in Europe by Nutrition in Adolescence study.CRF was measured by means of the shuttle run test,while fatness measures included body mass index(BMI),waist to height ratio,and fat mass index estimated from skinfold thicknesses.A clustered CMRs was computed by summing the standardized values of homeostasis model assessment,systolic blood pressure,triglycerides,total cholesterol/highdensity lipoprotein cholesterol ratio,and leptin.Results:Linear regression models indicated that CRF acted as an important and partial mediator in the association between fatness and CMRs in 1217-year-old adolescents(for BMI:coefficients of the indirect role b=0.058(95%confidence interval(95%CI):0.023-0.101),Sobel test z=3.11(10.0%mediation);for waist to height ratio:b=4.279(95%CI:2.242-7.059),z=3.86(11.5%mediation);and for fat mass index:b=0.060(95%CI:0.020-0.106),z=2.85(9.4%mediation);all p<0.01).Conclusion:In adolescents,the association between fatness and CMRs could be partially decreased with improvements to fitness levels;therefore,CRF contribution both in the clinical field and public health could be important to consider and promote in adolescents independently of their fatness levels.
文摘In this work, a grape phenolic extract obtained by methanol extraction has been demonstrated to be effective in inhibiting the growth of different strains and species of Campylobacter, one of the most important bacterial foodborne pathogens causing gastroenteritis worldwide. Noteworthily, it was particularly effective against several strains presenting multiple antibiotic resistances. In all cases, the minimum inhibitory concentration (MIC) was lower than 300 mg GAE/L, being of 60 mg GAE/L for one of the most resistant strains (C. coli LP2), while the others were between 120 mg GAE/L and 180 mg GAE/L. The analytical study of the main phenolic compounds in the grape extract revealed that it was mainly constituted by catechins (85.7%) and phenolic acids (13.7%). However, experiments developed using pure standards demonstrate that phenolic acids (such as gallic, p-hidroxibenzoic, vanillic, and homovanillic acids) were the most active, provoking a Campylobacter growth decrease between 6.7 and 7.6 log, while epicatechin was the only catechin with activity as pure compound (1 log of growth decrease).
基金grants from Agencia Nacional de Promoción Científica y Tecnológica(ANPCyT,PICT 2019-1697 and PICT 2019-0037)Secretaría de Ciencia,Arte e Innovación Tecnológica de la Universidad Nacional de Tucumán(PIUNT 26/G634)+1 种基金Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET,PIP 691)Consejo Superior de Investigaciones Científicas(CSIC,i-COOP-2021-COOPB20618),which funded this research。
文摘This study investigated the ability of Lactiplantibacillus paraplantarum CRL2051 and Lactiplantibacillus plantarum CRL2030,two lactic acid bacteria isolated from fruits,to ferment pomegranate juice to produce beverages with extended shelf life and functional properties.Lactic acid bacteria consumed glucose and fructose as carbon sources,producing mainly lactic acid.Both strains were initially grown in juice at 30℃ for 48 h,then refrigerated at 4℃ for 28 days.During fermentation and cold storage,the total phenolic content and antioxidant capacity were conserved in fermented pomegranate juices.The functionality studies of the fermented beverages administered to C57BL/6 mice fed a high-fat diet were evaluated over 6 weeks.Pomegranate juice supplementation led to a marked improvement in glucose and triglycerides serum profile,which decreased around 40%and up to 48%,respectively,and counteracted fat deposition and body weight gain.The high-fat diet treatment significantly incremented the aspartate aminotransferase activity in the liver,whereas fermented pomegranate juice consumption slightly reduced it.Liver histological changes associated with a high-fat diet were significantly reverted in juice-treated animals.Our findings evidenced the biological benefits of a novel functional beverage obtained by lactic fermentation of pomegranate juice;this functional drink may protect against weight gain,liver damage,and dyslipidemia induced by a high-fat diet consumption.
