To avoid the direct contact with mouse cells and possible heterogeneous pathogen in future application, we need to replace mouse embryonic fibroblasts with human fibroblasts as the feeder layer to maintain human embry...To avoid the direct contact with mouse cells and possible heterogeneous pathogen in future application, we need to replace mouse embryonic fibroblasts with human fibroblasts as the feeder layer to maintain human embryonic stem cells growth in the undifferentiated state. We success-fully use human fibroblasts derived from aborted fetus and adult prepuce as feeder layer to maintain human embryonic stem cells growth. During the passage and growth on this feeder layer, the human embryonic stem cells can keep their undifferentiated state.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.30030070 and 3010403048)the National Major Basic Research Project(Grant No.001CB51010)+1 种基金the Natural Science Fund of Hunan Province(Grant No.00JJY2036)the Science and Technology Conquest of Hunan Province(Grant No.01SSY1001).
文摘To avoid the direct contact with mouse cells and possible heterogeneous pathogen in future application, we need to replace mouse embryonic fibroblasts with human fibroblasts as the feeder layer to maintain human embryonic stem cells growth in the undifferentiated state. We success-fully use human fibroblasts derived from aborted fetus and adult prepuce as feeder layer to maintain human embryonic stem cells growth. During the passage and growth on this feeder layer, the human embryonic stem cells can keep their undifferentiated state.
