AIM:To identify genetic defects in a Chinese family with congenital posterior polar cataracts and assess the pathogenicity.METHODS:A four-generation Chinese family affected with autosomal dominant congenital cataract ...AIM:To identify genetic defects in a Chinese family with congenital posterior polar cataracts and assess the pathogenicity.METHODS:A four-generation Chinese family affected with autosomal dominant congenital cataract was recruited.Nineteen individuals took part in this study including 5 affected and 14 unaffected individuals.Sanger sequencing targeted hot-spot regions of 27 congenital cataract-causing genes for variant discovery.The pathogenicity of the variant was evaluated by the guidelines of American College of Medical Genetics and InterVar software.Confocal microscopy was applied to detect the subcellular localization of fluorescence-labeled ephrin type-A receptor 2(EPHA2).Co-immunoprecipitation assay was implemented to estimate the interaction between EphA2 and other lens membrane proteins.The mRNA and protein expression were analyzed by reverse transcription-polymerase chain reaction(qRT-PCR)and Western blotting assay,respectively.The cell migration was analyzed by wound healing assay.Zebrafish model was generated by ectopic expression of human EPHA2/p.R957P mutant to demonstrate whether the mutant could cause lens opacity in vivo.RESULTS:A novel missense and pathogenic variant c.2870G>C was identified in the sterile alpha motif(SAM)domain of EPHA2.Functional studies demonstrated the variant’s impact:reduced EPHA2 protein expression,altered subcellular localization,and disrupted interactions with other lens membrane proteins.This mutant notably enhanced human lens epithelial cell migration,and induced a central cloudy region and roughness in zebrafish lenses with ectopic expression of human EPHA2/p.R957P mutant under differential interference contrast(DIC)optics.CONCLUSION:Novel pathogenic c.2870G>C variant of EPHA2 in a Chinese congenital cataract family contributes to disease pathogenesis.展开更多
Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermed...Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.展开更多
The concentration of cell-free fetal DNA fragments should be detected before noninvasive prenatal testing(NIPT).The fetal DNA molecules have significant clinical potential in determining the overall performance of NIP...The concentration of cell-free fetal DNA fragments should be detected before noninvasive prenatal testing(NIPT).The fetal DNA molecules have significant clinical potential in determining the overall performance of NIPT and clinical interpretation.It is important to measure fetal DNA fraction before NIPT.However,there is still little research on how to calculate the concentration of female fetuses.Two estimation approaches were proposed to calculate fetal DNA fraction,including the fragments size-based approach,aneuploid-based approach,which are all approaches based on chromosome segments.Based on high-throughput sequencing data,two approaches to calculate the DNA fraction of male fetuses were tested and obtained the experiment values,which were close to the actual values.The correlation coefficient of fragments size-based approach was 0.9243(P<0.0001)and the aneuploid-based approach reached 0.9339(P<0.0001).We calculated the concentration of female fetuses and obtained remarkable experimental results.We came up with two approaches for calculating the fetal DNA fraction of female fetuses.It provides an important theoretical basis for the detection of female fetal concentration in future clinical diagnosis.展开更多
1 Introduction The rapidly growing protein structure data is a valuable biological resource and has been a focus of research in the academic community.The abundance of 3D structure data also presents challenges for re...1 Introduction The rapidly growing protein structure data is a valuable biological resource and has been a focus of research in the academic community.The abundance of 3D structure data also presents challenges for researchers in terms of handling and analysis.Currently,the most popular format for bioinformatics programs as the input/output is the PDB format,a text-based format created in 1976 known for its clear and readable structure.Parsing PDB files provides crucial information about the structure,function,and interactions of biomolecules.展开更多
Previous studies have sought to classify bladder cancer(BLCA)into different molecular subtypes to understand its pathogenic pathways and uncover specific treatments.1 These subtypes,often based on genetic,transcriptom...Previous studies have sought to classify bladder cancer(BLCA)into different molecular subtypes to understand its pathogenic pathways and uncover specific treatments.1 These subtypes,often based on genetic,transcriptomic,or proteomic profiles,aim to stratify patients for precision medicine and improve therapeutic outcomes.Despite these efforts,such classifications have rarely been applied in clinical practice due to challenges in standardization,reproducibility,and limited translational studies validating their utility.1 The treatment of BLCA predominantly relies on surgery,often combined with chemotherapy,immunotherapy,targeted therapy,or antibody-drug conjugates.Radical cystectomy remains the cornerstone for muscle-invasive bladder cancer(MIBC),while transurethral resection and intravesical therapy are common for non-muscle-invasive bladder cancer(NMIBC).2 However,the choice of its treatment modality still depends specifically on whether the disease is NMIBC or MIBC,rather than on the various molecular subtype classifications.3 Bridging the gap between molecular research and clinical application remains a significant challenge,highlighting the need for robust biomarker validation and the development of treatment algorithms that incorporate these subtypes to better guide personalized therapy.展开更多
Exosomes encompass a great deal of valuable biological information and play a crit-ical role in tumor development.However,the mechanism of exosomal lncRNAs remains poorly elucidated in bladder cancer(BCa).In this stud...Exosomes encompass a great deal of valuable biological information and play a crit-ical role in tumor development.However,the mechanism of exosomal lncRNAs remains poorly elucidated in bladder cancer(BCa).In this study,we identified exosomal lnc-TAF12e2:1 as a novel biomarker in BCa diagnosis and aimed to investigate the underlying biological function.Dual luciferase reporter assay,RNA immunoprecipitation(RIP),RNA pulldown assays,and xenograft mouse model were used to verify the competitive endogenous RNA mechanism of lnc-TAF12e2:1.We found exosomal lnc-TAF12e2:1 up-regulated in urinary exosomes,tumor tissues of patients,and BCa cells.Down-regulation of lnc-TAF12e2:1 impaired BCa cell prolif-eration and migration,and promoted cell cycle arrest at the G0/G1 phase and cell apoptosis.The opposite effects were also observed when lnc-TAF12e2:1 was overexpressed.lnc-TAF12 e2:1 was transferred by intercellular exosomes to modulate malignant biological behavior.Mechanistically,lnc-TAF12e2:1 packaged in the exosomes relieved the miRNA-mediated silence effect on ASB12 via serving as a sponger of miR-7847e3p to accelerate progression in BCa.ASB12 was also first proved as an oncogene to promote cell proliferation and migration and depress cell cycle arrest and cell apoptosis in our data.In conclusion,exosomal lnc-TAF12e2:1,located in the cytoplasm of BCa,might act as a competitive endogenous RNA to competitively bind to miR-7847e3p,and then be involved in miR-7847e3p/ASB12 regulatory axis to promote tumorigenesis,which provided a deeper insight into the molecular mechanism of BCa.展开更多
Pancreaticβcells differentiated from stem cells provide promise for cell replacement therapy of diabetes.Human pluripotent stem cells could be differentiated into definitive endoderm,followed by pancreatic progenitor...Pancreaticβcells differentiated from stem cells provide promise for cell replacement therapy of diabetes.Human pluripotent stem cells could be differentiated into definitive endoderm,followed by pancreatic progenitors,and then subjected to endocrinal differentiation and maturation in a stepwise fashion.Many achievements have been made in making pancreaticβcells from human pluripotent stem cells in last two decades,and a couple of phase I/II clinical trials have just been initiated.Here,we overview the major progresses in differentiating pancreaticβcells from human pluripotent stem cells with the focus on recent technical advances in each differentiation stage,and briefly discuss the current limitations as well.展开更多
Following publication of the original article(Jin&Jiang,2022),it was reported that the word“financial”needs to be removed from the Competing interests section.The original sentence was:The authors declare no com...Following publication of the original article(Jin&Jiang,2022),it was reported that the word“financial”needs to be removed from the Competing interests section.The original sentence was:The authors declare no competing financial interests.展开更多
Summary What is already known about this topic?The significance of maternal liver health concerning preterm birth(PTB)is well recognized;however,there is a gap in understanding the precise influence of preconception s...Summary What is already known about this topic?The significance of maternal liver health concerning preterm birth(PTB)is well recognized;however,there is a gap in understanding the precise influence of preconception serum alanine aminotransferase(ALT)levels on the risk of PTB.What is added by this report?展开更多
Life expectancy is increasing,leading to the continuous aging of the population in China.Enhancing the health status of the older population is crucial to achieving healthy aging.The primary objective of the PENG ZU S...Life expectancy is increasing,leading to the continuous aging of the population in China.Enhancing the health status of the older population is crucial to achieving healthy aging.The primary objective of the PENG ZU Study on Healthy Aging in China(PENG ZU Cohort)is to understand the natural progression of health status among the aging Chinese population.Specifically,the PENG ZU cohort aims to identify and validate multidimensional aging markers,uncover the underlying mechanisms of systemic aging and functional decline,and develop novel strategies and measures to delay functional decline and adverse health outcomes,while maintaining overall good health.The PENG ZU cohort consists of 26,000 individuals aged 25 to 89 years from seven major geographical regions in China.Diversified data and biospecimens are collected according to standardized procedures at baseline and follow-up visits.Baseline recruitment for the PENG ZU cohort was completed in October 2021.The extensive analysis of multidimensional health-related data and bioresources collected from the cohort is anticipated to develop methods for evaluating functional status and elucidating multilevel,cross-scale interactions and regulatory mechanisms of healthy aging.The findings from this study will enhance the understanding of health changes due to aging,facilitate efficient and effective interventions to maintain functional ability,and reduce the incidence and severity of age-related diseases,thereby further promoting healthy aging.展开更多
Background:Psychological stress has been reported to be a potential risk factor for hypertension among females,but it remains unclear whether spousal chronic stress levels alter the risk of hypertension among women.We...Background:Psychological stress has been reported to be a potential risk factor for hypertension among females,but it remains unclear whether spousal chronic stress levels alter the risk of hypertension among women.We examined the associations between stress within the family and hypertension among married women.Methods:Reproductive-aged women who were planning for pregnancy and their husbands were recruited from the National Free Pre-pregnancy Checkup Projects(NFPCP)across 31 provinces in China in 2016 and 2017.Perceived stress of wives or husbands was measured with a 5-point Likert-type scale,and assessed from three domains:work/life-related stress,economic stress,and overall stress.Multivariable-adjusted logistic regression models were used to assess the associations between stress status and the prevalence of hypertension.Results:Of 10,027,644 couples,261,098(2.60%)women had hypertension.The results showed that higher stress levels among themselves or their husbands were associated with a higher prevalence of hypertension in women(P_(for trend)<0.001).Compared with non-stressed participants,female participants with the highest stress themselves were at a greater risk of hypertension,with adjusted odds ratio(OR)of 1.31(95%confidence interval[CI]:1.25-1.37);and compared with participants whose husbands had no stress,those whose husbands had the highest stress level were at a higher risk of hypertension with adjusted OR of 1.24(95%CI:1.20-1.29).Moreover,compared with non-stressed status for both couples,only-wife-stressed,only-husband-stressed,and both-stressed couples were found to be significantly associated with increased risks of wives’hypertension,with adjusted ORs of 1.28(95%CI:1.25-1.31),1.19(95%CI:1.17-1.21),and 1.28(95%CI:1.26-1.31),respectively.Conclusion:Moderate to severe stress in both spouses might be associated with female hypertension prevalence,which highlights the importance of paying attention to the psychological stresses of couples within the family.展开更多
Introduction:Biological age(BA)can represent the actual state of human aging more accurately than chronological age(CA).Methods:Using hematological data from 112,925 participants in southwestern China,collected betwee...Introduction:Biological age(BA)can represent the actual state of human aging more accurately than chronological age(CA).Methods:Using hematological data from 112,925 participants in southwestern China,collected between 2015 and 2021,this study constructed BA predictors using 7 machine learning(ML)methods(tailored separately for male and female populations).This study then analyzed the association between BA acceleration and type 2 diabetes mellitus(T2DM)within this data using logistic regression.Additionally,it examined the impact of glycemic control on BA in individuals with diabetes.Results:Among all ML models,deep neural networks(DNN)delivered the best performance in male[mean absolute error(MAE)=6.89,r=0.75]and female subsets(MAE=6.86,r=0.74).BA acceleration showed positive correlations with T2DM in both male[odds ratio(OR):2.22,95%confidence interval(CI):1.77–2.77]and female subsets(OR:3.10,95%CI:2.16–4.46),while BA deceleration showed negative correlations in both male(OR:0.32,95%CI:0.27–0.39)and female subsets(OR:0.42,95%CI:0.33–0.53).Individuals with diabetes with normal fasting glucose had significantly lower BAs than those with impaired fasting glucose in all CA groups except for patients older than 80.Discussion:Artificial intelligence(AI)-based hematological BA predictors show promise as advanced tools for assessing aging in epidemiological studies.Implementing AI-based BA predictors in public health initiatives could facilitate proactive aging management and disease prevention.展开更多
Summary What is already known about this topic?Hematological parameters may indicate the presence of chronic low-grade inflammation and increasing viscosity,which are involved in the pathological processes of gestatio...Summary What is already known about this topic?Hematological parameters may indicate the presence of chronic low-grade inflammation and increasing viscosity,which are involved in the pathological processes of gestational diabetes mellitus(GDM).However,the association between several hematological parameters in early pregnancy and GDM has yet to be elucidated.What is added by this report?Hematological parameters in the first trimester,particularly red blood cell(RBC)count and systematic immune index,have a significant impact on GDM incidence.The neutrophils(NEU)count in the first trimester was particularly pronounced for GDM.The upward trend of RBC,white blood cell(WBC),and NEU counts was consistent across all GDM subtypes.What are the implications for public health practice?Early pregnancy hematological parameters are associated with the risk of GDM.展开更多
Exposure to greenness may lead to a wide range of beneficial health outcomes.However,the effects of greenness on preterm birth(PTB)are inconsistent,and limited studies have focused on the subcategories of PTB.A total ...Exposure to greenness may lead to a wide range of beneficial health outcomes.However,the effects of greenness on preterm birth(PTB)are inconsistent,and limited studies have focused on the subcategories of PTB.A total of 3,751,672 singleton births from a national birth cohort in China's Mainland were included in this study.Greenness was estimated using the satellitebased Normalized Difference Vegetation Index(NDVI)and Enhanced Vegetation Index with 500-m and 1,000-m buffers around participants’addresses.The subcategories of PTB(20-36 weeks)included extremely PTB(EPTB,20-27 weeks).展开更多
Bladder cancer(BCa)is the ninth most frequently diagnosed tumor worldwide,and smoking remains a major risk faaor.BCa is divided into nonmuscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC).1 N...Bladder cancer(BCa)is the ninth most frequently diagnosed tumor worldwide,and smoking remains a major risk faaor.BCa is divided into nonmuscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC).1 NMIBC accounts for 70-80% of all BCa cases,its recurrence rate is high,and 20-30% of NMIBC patients progress to MIBC.展开更多
Preterm birth(PTB)is a leading cause of perinatal mortality and morbidity worldwide[1].Since maternal and fetal health protection has been included in the United Nations Sustainable Development Goals,identification of...Preterm birth(PTB)is a leading cause of perinatal mortality and morbidity worldwide[1].Since maternal and fetal health protection has been included in the United Nations Sustainable Development Goals,identification of modifiable risk factors for PTB is particularly crucial.The Global Burden of Disease(GBD)Study 2019 recognized fine particulate matter(PM2.5)pollution as a risk factor for decreased gestational age and low birth weight.展开更多
The composition of these vaginal microbiome has a significant impact on women’s health.However,few studies have characterized the vaginal microbiome of healthy Chinese women using metagenomic sequencing.Here,we carri...The composition of these vaginal microbiome has a significant impact on women’s health.However,few studies have characterized the vaginal microbiome of healthy Chinese women using metagenomic sequencing.Here,we carried out a comparative metagenomic analysis to survey taxonomic,functional levels,and microbial communities’genome content in healthy women’s vaginal microbiome.Overall,we observed a total of 111 species,including all dominant vaginal Lactobacillus species,such as L.iners,L.crispatus,L.gasseri,and L.jensenii.Unlike microbial taxa,several pathways were ubiquitous and prevalent across individuals,including adenosine ribonucleotides de novo biosynthesis and pyruvate fermentation to acetate and lactate Ⅱ.Notably,our diversity analysis confirmed a significant difference in healthy women from different ethnic groups.Moreover,we binned vaginal assemblies into 62 high-quality genomes,including 9 L.iners,7 A.vaginae,5 L.jensenii,and 5 L.crispatus.We identified the pan and core genomes of L.iners and A.vaginae and revealed the genetic diversity.Primary differences between strains were the hypothetical genes and mobile element-like genes.Our results provide a framework for understanding the implications of the female reproductive tract’s composition and functional potential and highlight the importance of genome-resolved metagenomic analysis to further understand the human vaginal microbiome.展开更多
基金Supported by the Natural Science Foundation of Fujian Province(No.2021J01229)National Key Research and Development Program of China(No.2016YFC1000307).
文摘AIM:To identify genetic defects in a Chinese family with congenital posterior polar cataracts and assess the pathogenicity.METHODS:A four-generation Chinese family affected with autosomal dominant congenital cataract was recruited.Nineteen individuals took part in this study including 5 affected and 14 unaffected individuals.Sanger sequencing targeted hot-spot regions of 27 congenital cataract-causing genes for variant discovery.The pathogenicity of the variant was evaluated by the guidelines of American College of Medical Genetics and InterVar software.Confocal microscopy was applied to detect the subcellular localization of fluorescence-labeled ephrin type-A receptor 2(EPHA2).Co-immunoprecipitation assay was implemented to estimate the interaction between EphA2 and other lens membrane proteins.The mRNA and protein expression were analyzed by reverse transcription-polymerase chain reaction(qRT-PCR)and Western blotting assay,respectively.The cell migration was analyzed by wound healing assay.Zebrafish model was generated by ectopic expression of human EPHA2/p.R957P mutant to demonstrate whether the mutant could cause lens opacity in vivo.RESULTS:A novel missense and pathogenic variant c.2870G>C was identified in the sterile alpha motif(SAM)domain of EPHA2.Functional studies demonstrated the variant’s impact:reduced EPHA2 protein expression,altered subcellular localization,and disrupted interactions with other lens membrane proteins.This mutant notably enhanced human lens epithelial cell migration,and induced a central cloudy region and roughness in zebrafish lenses with ectopic expression of human EPHA2/p.R957P mutant under differential interference contrast(DIC)optics.CONCLUSION:Novel pathogenic c.2870G>C variant of EPHA2 in a Chinese congenital cataract family contributes to disease pathogenesis.
基金supported by the National Natural Science Foundation of China(21878324,21706273)the CAS Pioneer Hundred Talent Program(Type C,reference#2016-081)。
文摘Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.
基金supported by the National Key Research and Development Program of China 2016YFC1000307The sub-project of National Key Research and Development Program of China 2016YFC1000307-10the Program of National Research Institute for Family Planning(2017GJM04,2018CNV).
文摘The concentration of cell-free fetal DNA fragments should be detected before noninvasive prenatal testing(NIPT).The fetal DNA molecules have significant clinical potential in determining the overall performance of NIPT and clinical interpretation.It is important to measure fetal DNA fraction before NIPT.However,there is still little research on how to calculate the concentration of female fetuses.Two estimation approaches were proposed to calculate fetal DNA fraction,including the fragments size-based approach,aneuploid-based approach,which are all approaches based on chromosome segments.Based on high-throughput sequencing data,two approaches to calculate the DNA fraction of male fetuses were tested and obtained the experiment values,which were close to the actual values.The correlation coefficient of fragments size-based approach was 0.9243(P<0.0001)and the aneuploid-based approach reached 0.9339(P<0.0001).We calculated the concentration of female fetuses and obtained remarkable experimental results.We came up with two approaches for calculating the fetal DNA fraction of female fetuses.It provides an important theoretical basis for the detection of female fetal concentration in future clinical diagnosis.
基金supported by the National Natural Science Foundation of China(Grant No.81973243)the National Human Genetic Resources Center,National Research Institute for Family Planning。
文摘1 Introduction The rapidly growing protein structure data is a valuable biological resource and has been a focus of research in the academic community.The abundance of 3D structure data also presents challenges for researchers in terms of handling and analysis.Currently,the most popular format for bioinformatics programs as the input/output is the PDB format,a text-based format created in 1976 known for its clear and readable structure.Parsing PDB files provides crucial information about the structure,function,and interactions of biomolecules.
基金supported by the National Natural Science Foundation of China(No.82273065)Fundamental Research Funds for the Central Universities(China)(No.2042022dx0003)+1 种基金Research Fund of Zhongnan Hospital of Wuhan University(Wuhan,China)(No.YYXKNLJS2024001,PTPP2024001)The funders played no role in the study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Previous studies have sought to classify bladder cancer(BLCA)into different molecular subtypes to understand its pathogenic pathways and uncover specific treatments.1 These subtypes,often based on genetic,transcriptomic,or proteomic profiles,aim to stratify patients for precision medicine and improve therapeutic outcomes.Despite these efforts,such classifications have rarely been applied in clinical practice due to challenges in standardization,reproducibility,and limited translational studies validating their utility.1 The treatment of BLCA predominantly relies on surgery,often combined with chemotherapy,immunotherapy,targeted therapy,or antibody-drug conjugates.Radical cystectomy remains the cornerstone for muscle-invasive bladder cancer(MIBC),while transurethral resection and intravesical therapy are common for non-muscle-invasive bladder cancer(NMIBC).2 However,the choice of its treatment modality still depends specifically on whether the disease is NMIBC or MIBC,rather than on the various molecular subtype classifications.3 Bridging the gap between molecular research and clinical application remains a significant challenge,highlighting the need for robust biomarker validation and the development of treatment algorithms that incorporate these subtypes to better guide personalized therapy.
基金funded by Wuhan University(No.4206e413100049)the Science and Technology Department of Hubei Province Key Project(China)(No.2022EJD001)+3 种基金Zhongnan Hospital of Wuhan University(No.ZNYQ2023002,KY0100000109)the Fundamental Research Funds for the Central Universities(China)(No.2042022dx0003)Nonprofit Central Research Institute Fund of Chinese Academy of Medical Sciences(No.2020-PT320-004)Young Elite Scientists Sponsorship Program by the China Association for Science and Technology(No.2022QNRC001).
文摘Exosomes encompass a great deal of valuable biological information and play a crit-ical role in tumor development.However,the mechanism of exosomal lncRNAs remains poorly elucidated in bladder cancer(BCa).In this study,we identified exosomal lnc-TAF12e2:1 as a novel biomarker in BCa diagnosis and aimed to investigate the underlying biological function.Dual luciferase reporter assay,RNA immunoprecipitation(RIP),RNA pulldown assays,and xenograft mouse model were used to verify the competitive endogenous RNA mechanism of lnc-TAF12e2:1.We found exosomal lnc-TAF12e2:1 up-regulated in urinary exosomes,tumor tissues of patients,and BCa cells.Down-regulation of lnc-TAF12e2:1 impaired BCa cell prolif-eration and migration,and promoted cell cycle arrest at the G0/G1 phase and cell apoptosis.The opposite effects were also observed when lnc-TAF12e2:1 was overexpressed.lnc-TAF12 e2:1 was transferred by intercellular exosomes to modulate malignant biological behavior.Mechanistically,lnc-TAF12e2:1 packaged in the exosomes relieved the miRNA-mediated silence effect on ASB12 via serving as a sponger of miR-7847e3p to accelerate progression in BCa.ASB12 was also first proved as an oncogene to promote cell proliferation and migration and depress cell cycle arrest and cell apoptosis in our data.In conclusion,exosomal lnc-TAF12e2:1,located in the cytoplasm of BCa,might act as a competitive endogenous RNA to competitively bind to miR-7847e3p,and then be involved in miR-7847e3p/ASB12 regulatory axis to promote tumorigenesis,which provided a deeper insight into the molecular mechanism of BCa.
基金This work was supported by the National Natural Science Foundation of China(91740102)the Science and Technology Department of Hubei Province(2021CFA049)+1 种基金Health Commission of Hubei Province scientific research project(WJ2021Q029)the Fundamental Research Funds for the Central Universities in China(2042021kf0207).
文摘Pancreaticβcells differentiated from stem cells provide promise for cell replacement therapy of diabetes.Human pluripotent stem cells could be differentiated into definitive endoderm,followed by pancreatic progenitors,and then subjected to endocrinal differentiation and maturation in a stepwise fashion.Many achievements have been made in making pancreaticβcells from human pluripotent stem cells in last two decades,and a couple of phase I/II clinical trials have just been initiated.Here,we overview the major progresses in differentiating pancreaticβcells from human pluripotent stem cells with the focus on recent technical advances in each differentiation stage,and briefly discuss the current limitations as well.
文摘Following publication of the original article(Jin&Jiang,2022),it was reported that the word“financial”needs to be removed from the Competing interests section.The original sentence was:The authors declare no competing financial interests.
基金Supported by the National Key Research and Development Program of China(NKPs)under grants 2021YFC2700705(Ms.Yang)and 2016YFC100307(Mr.Ma).
文摘Summary What is already known about this topic?The significance of maternal liver health concerning preterm birth(PTB)is well recognized;however,there is a gap in understanding the precise influence of preconception serum alanine aminotransferase(ALT)levels on the risk of PTB.What is added by this report?
基金Supported by the National Key Research and Development Program of China(2018YFC2000300,2020YFC2002700).
文摘Life expectancy is increasing,leading to the continuous aging of the population in China.Enhancing the health status of the older population is crucial to achieving healthy aging.The primary objective of the PENG ZU Study on Healthy Aging in China(PENG ZU Cohort)is to understand the natural progression of health status among the aging Chinese population.Specifically,the PENG ZU cohort aims to identify and validate multidimensional aging markers,uncover the underlying mechanisms of systemic aging and functional decline,and develop novel strategies and measures to delay functional decline and adverse health outcomes,while maintaining overall good health.The PENG ZU cohort consists of 26,000 individuals aged 25 to 89 years from seven major geographical regions in China.Diversified data and biospecimens are collected according to standardized procedures at baseline and follow-up visits.Baseline recruitment for the PENG ZU cohort was completed in October 2021.The extensive analysis of multidimensional health-related data and bioresources collected from the cohort is anticipated to develop methods for evaluating functional status and elucidating multilevel,cross-scale interactions and regulatory mechanisms of healthy aging.The findings from this study will enhance the understanding of health changes due to aging,facilitate efficient and effective interventions to maintain functional ability,and reduce the incidence and severity of age-related diseases,thereby further promoting healthy aging.
基金National Key Research and Development Program of China(No.2016YFC1000307)
文摘Background:Psychological stress has been reported to be a potential risk factor for hypertension among females,but it remains unclear whether spousal chronic stress levels alter the risk of hypertension among women.We examined the associations between stress within the family and hypertension among married women.Methods:Reproductive-aged women who were planning for pregnancy and their husbands were recruited from the National Free Pre-pregnancy Checkup Projects(NFPCP)across 31 provinces in China in 2016 and 2017.Perceived stress of wives or husbands was measured with a 5-point Likert-type scale,and assessed from three domains:work/life-related stress,economic stress,and overall stress.Multivariable-adjusted logistic regression models were used to assess the associations between stress status and the prevalence of hypertension.Results:Of 10,027,644 couples,261,098(2.60%)women had hypertension.The results showed that higher stress levels among themselves or their husbands were associated with a higher prevalence of hypertension in women(P_(for trend)<0.001).Compared with non-stressed participants,female participants with the highest stress themselves were at a greater risk of hypertension,with adjusted odds ratio(OR)of 1.31(95%confidence interval[CI]:1.25-1.37);and compared with participants whose husbands had no stress,those whose husbands had the highest stress level were at a higher risk of hypertension with adjusted OR of 1.24(95%CI:1.20-1.29).Moreover,compared with non-stressed status for both couples,only-wife-stressed,only-husband-stressed,and both-stressed couples were found to be significantly associated with increased risks of wives’hypertension,with adjusted ORs of 1.28(95%CI:1.25-1.31),1.19(95%CI:1.17-1.21),and 1.28(95%CI:1.26-1.31),respectively.Conclusion:Moderate to severe stress in both spouses might be associated with female hypertension prevalence,which highlights the importance of paying attention to the psychological stresses of couples within the family.
基金Supported by the National Human Genetic Resources Sharing Service Platform(Grant No.2005DKA21300).
文摘Introduction:Biological age(BA)can represent the actual state of human aging more accurately than chronological age(CA).Methods:Using hematological data from 112,925 participants in southwestern China,collected between 2015 and 2021,this study constructed BA predictors using 7 machine learning(ML)methods(tailored separately for male and female populations).This study then analyzed the association between BA acceleration and type 2 diabetes mellitus(T2DM)within this data using logistic regression.Additionally,it examined the impact of glycemic control on BA in individuals with diabetes.Results:Among all ML models,deep neural networks(DNN)delivered the best performance in male[mean absolute error(MAE)=6.89,r=0.75]and female subsets(MAE=6.86,r=0.74).BA acceleration showed positive correlations with T2DM in both male[odds ratio(OR):2.22,95%confidence interval(CI):1.77–2.77]and female subsets(OR:3.10,95%CI:2.16–4.46),while BA deceleration showed negative correlations in both male(OR:0.32,95%CI:0.27–0.39)and female subsets(OR:0.42,95%CI:0.33–0.53).Individuals with diabetes with normal fasting glucose had significantly lower BAs than those with impaired fasting glucose in all CA groups except for patients older than 80.Discussion:Artificial intelligence(AI)-based hematological BA predictors show promise as advanced tools for assessing aging in epidemiological studies.Implementing AI-based BA predictors in public health initiatives could facilitate proactive aging management and disease prevention.
基金Supported by the National Key Research and Development Program of China(NKPs)[Grant no.2021YFC2700705].
文摘Summary What is already known about this topic?Hematological parameters may indicate the presence of chronic low-grade inflammation and increasing viscosity,which are involved in the pathological processes of gestational diabetes mellitus(GDM).However,the association between several hematological parameters in early pregnancy and GDM has yet to be elucidated.What is added by this report?Hematological parameters in the first trimester,particularly red blood cell(RBC)count and systematic immune index,have a significant impact on GDM incidence.The neutrophils(NEU)count in the first trimester was particularly pronounced for GDM.The upward trend of RBC,white blood cell(WBC),and NEU counts was consistent across all GDM subtypes.What are the implications for public health practice?Early pregnancy hematological parameters are associated with the risk of GDM.
基金This work was supported by the National Key Research and Development Program of China(2016YFC1000102 and 2016YFC1000307),and supported by a grant from the State Key Laboratory of Resources and the Environmental Information System.
文摘Exposure to greenness may lead to a wide range of beneficial health outcomes.However,the effects of greenness on preterm birth(PTB)are inconsistent,and limited studies have focused on the subcategories of PTB.A total of 3,751,672 singleton births from a national birth cohort in China's Mainland were included in this study.Greenness was estimated using the satellitebased Normalized Difference Vegetation Index(NDVI)and Enhanced Vegetation Index with 500-m and 1,000-m buffers around participants’addresses.The subcategories of PTB(20-36 weeks)included extremely PTB(EPTB,20-27 weeks).
基金supported in part by grants from the Fundamental Research Funds for the Central Universities(2042018kf1040)the Chinese Central Special Fund for Local Science and Technology Development of Hubei Province(2018ZYYD023)+1 种基金the Science and Technology Department of Hubei Province Key Project(2018ACA159)the Wuhan Science and Technology Bureau Key Project(2018061005132294).
文摘Bladder cancer(BCa)is the ninth most frequently diagnosed tumor worldwide,and smoking remains a major risk faaor.BCa is divided into nonmuscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC).1 NMIBC accounts for 70-80% of all BCa cases,its recurrence rate is high,and 20-30% of NMIBC patients progress to MIBC.
基金supported by the National Key Research and Development Program of China(2016YFC1000102,2016YFC1000307,and 2016YFC0206202)National Natural Science Foundation of China(91543114)+1 种基金Natural Science Foundation of Shanghai(20ZR1402900)a grant from State Key Laboratory of Resources and Environmental Information System。
文摘Preterm birth(PTB)is a leading cause of perinatal mortality and morbidity worldwide[1].Since maternal and fetal health protection has been included in the United Nations Sustainable Development Goals,identification of modifiable risk factors for PTB is particularly crucial.The Global Burden of Disease(GBD)Study 2019 recognized fine particulate matter(PM2.5)pollution as a risk factor for decreased gestational age and low birth weight.
基金supported in part by the National Key Research and Development Program of China(2017YFD0400602 and 2016YFC1000102-2)the National Natural Science Foundation of China(31500114)the Beijing Municipal Natural Science Foundation(7192169).
文摘The composition of these vaginal microbiome has a significant impact on women’s health.However,few studies have characterized the vaginal microbiome of healthy Chinese women using metagenomic sequencing.Here,we carried out a comparative metagenomic analysis to survey taxonomic,functional levels,and microbial communities’genome content in healthy women’s vaginal microbiome.Overall,we observed a total of 111 species,including all dominant vaginal Lactobacillus species,such as L.iners,L.crispatus,L.gasseri,and L.jensenii.Unlike microbial taxa,several pathways were ubiquitous and prevalent across individuals,including adenosine ribonucleotides de novo biosynthesis and pyruvate fermentation to acetate and lactate Ⅱ.Notably,our diversity analysis confirmed a significant difference in healthy women from different ethnic groups.Moreover,we binned vaginal assemblies into 62 high-quality genomes,including 9 L.iners,7 A.vaginae,5 L.jensenii,and 5 L.crispatus.We identified the pan and core genomes of L.iners and A.vaginae and revealed the genetic diversity.Primary differences between strains were the hypothetical genes and mobile element-like genes.Our results provide a framework for understanding the implications of the female reproductive tract’s composition and functional potential and highlight the importance of genome-resolved metagenomic analysis to further understand the human vaginal microbiome.
