BACKGROUND Colorectal cancer is the third most common malignancy and the fourth leading cause of cancer-related deaths worldwide.Several studies have shown an association between gut microbiota and colorectal cancer.G...BACKGROUND Colorectal cancer is the third most common malignancy and the fourth leading cause of cancer-related deaths worldwide.Several studies have shown an association between gut microbiota and colorectal cancer.Gut microbiota is unique and can be influenced by geographic factors and habits.This study aimed to determine the diversity and composition of colonic mucosal microbiota in patients with and without colorectal cancer.AIM To determine the diversity and composition of colonic mucosal microbiota in patients with and without colorectal cancer in Indonesia.METHODS This case-control study included 59 subjects(35 colorectal cancer patients and 24 non-colorectal cancer patients indicated for colonoscopy at Dr.Cipto Mangunkusumo Gastrointestinal Endoscopy Center and Fatmawati Hospital.Microbiota examination was performed using 16S rRNA sequencing.Bioinformatics analysis was performed using the wf-metagenomics pipeline from EPI2Me-Labs(Oxford Nanopore Technologies platform).RESULTS Patients with colorectal cancer had a higher median index value on the Shannon index(3.28 vs 2.82,P>0.05)and a lower value on the Simpson index(0.050 vs 0.060,P>0.05).Significant differences in beta diversity were observed at the genus(P=0.002)and species levels(P=0.001).Firmicutes,Proteobacteria,Bacteroidetes,and Fusobacteria were the dominant phyla.The genera Bacteroides,Campylobacter,Peptostreptococcus,and Parvimonas were found more frequently in colorectal cancer,while Faecalibacterium,Haemophilus,and Phocaeicola were more frequently found in non-colorectal cancer.The relative abundance of Fusobacterium nucleatum,Bacteroides fragilis,Enterococcus faecalis,Campylobacter hominis,and Enterococcus faecalis species was significantly elevated in patients with colorectal cancer.Meanwhile,Faecalibacterium prausnitzii,Faecalibacterium duncaniae,and Prevotella copri were more commonly found in non-colorectal cancer.CONCLUSION Patients with colorectal cancer exhibit distinct differences in the composition and diversity of their colonic mucosal microbiota compared to those with non-colorectal cancer.This study was reviewed and approved by the Ethics Committee of Faculty of Medicine,Universitas Indonesia(No.KET-1517/UN2.F1/ETIK/PPM.00.02/2023).展开更多
Background and Objectives:Intestinal permeability(IP)is known to contribute to the immune system activation and inflammation;thus,it is proposed to have a role in the pathogenesis and exacerbation of many chronic dise...Background and Objectives:Intestinal permeability(IP)is known to contribute to the immune system activation and inflammation;thus,it is proposed to have a role in the pathogenesis and exacerbation of many chronic diseases.Several studies have indicated that diet and nutritional status are risk factors for increased IP.In this mini review,we discussed the recent evidence on the association of diet,nutritional status,and intestinal permeability assessed by zonulin concentrations in serum and feces.Methods and Study Design:Literature searching was conducted in Pubmed,ProQuest and Google Scholar using the keywords“diet quality”,“intestinal permeability”,“nutritional status”,and“zonulin”combined with Boolean operators“AND”and“OR”.Results:Some studies indicated that intake of proper nutrition and good diet such as low total calorie intake,high intakes of omega-3 polyunsaturated fatty acids,fiber,vitamins,minerals,probiotics,and polyphenol-rich diet have significant impact on improvement of intestinal permeability marked by lower zonulin concentrations.Higher zonulin concentrations are found in those with overweight and obesity indicating that these population have increased IP.Most studies were conducted in adults and there are limited studies in children and adolescents.In addition,no studies have assessed diet quality to obtain a comprehensive picture on the complexities of diet in the population in relation to intestinal permeability.Conclusions:Diet and nutritional status are linked to zonulin concentrations,indicating a role in intestinal permeability.Further research should be conducted to investigate the relationship between diet quality,as measured by appropriate diet quality indices,and intestinal permeability in children,adolescents,and adults.展开更多
文摘BACKGROUND Colorectal cancer is the third most common malignancy and the fourth leading cause of cancer-related deaths worldwide.Several studies have shown an association between gut microbiota and colorectal cancer.Gut microbiota is unique and can be influenced by geographic factors and habits.This study aimed to determine the diversity and composition of colonic mucosal microbiota in patients with and without colorectal cancer.AIM To determine the diversity and composition of colonic mucosal microbiota in patients with and without colorectal cancer in Indonesia.METHODS This case-control study included 59 subjects(35 colorectal cancer patients and 24 non-colorectal cancer patients indicated for colonoscopy at Dr.Cipto Mangunkusumo Gastrointestinal Endoscopy Center and Fatmawati Hospital.Microbiota examination was performed using 16S rRNA sequencing.Bioinformatics analysis was performed using the wf-metagenomics pipeline from EPI2Me-Labs(Oxford Nanopore Technologies platform).RESULTS Patients with colorectal cancer had a higher median index value on the Shannon index(3.28 vs 2.82,P>0.05)and a lower value on the Simpson index(0.050 vs 0.060,P>0.05).Significant differences in beta diversity were observed at the genus(P=0.002)and species levels(P=0.001).Firmicutes,Proteobacteria,Bacteroidetes,and Fusobacteria were the dominant phyla.The genera Bacteroides,Campylobacter,Peptostreptococcus,and Parvimonas were found more frequently in colorectal cancer,while Faecalibacterium,Haemophilus,and Phocaeicola were more frequently found in non-colorectal cancer.The relative abundance of Fusobacterium nucleatum,Bacteroides fragilis,Enterococcus faecalis,Campylobacter hominis,and Enterococcus faecalis species was significantly elevated in patients with colorectal cancer.Meanwhile,Faecalibacterium prausnitzii,Faecalibacterium duncaniae,and Prevotella copri were more commonly found in non-colorectal cancer.CONCLUSION Patients with colorectal cancer exhibit distinct differences in the composition and diversity of their colonic mucosal microbiota compared to those with non-colorectal cancer.This study was reviewed and approved by the Ethics Committee of Faculty of Medicine,Universitas Indonesia(No.KET-1517/UN2.F1/ETIK/PPM.00.02/2023).
基金supported by Ministry of Education,Culture,Research,and Technology,Republic of Indonesia under the Doctoral Dissertation Research Grant[Grant No.NKB-306/UN2.RST/HKP.05.00/2021].
文摘Background and Objectives:Intestinal permeability(IP)is known to contribute to the immune system activation and inflammation;thus,it is proposed to have a role in the pathogenesis and exacerbation of many chronic diseases.Several studies have indicated that diet and nutritional status are risk factors for increased IP.In this mini review,we discussed the recent evidence on the association of diet,nutritional status,and intestinal permeability assessed by zonulin concentrations in serum and feces.Methods and Study Design:Literature searching was conducted in Pubmed,ProQuest and Google Scholar using the keywords“diet quality”,“intestinal permeability”,“nutritional status”,and“zonulin”combined with Boolean operators“AND”and“OR”.Results:Some studies indicated that intake of proper nutrition and good diet such as low total calorie intake,high intakes of omega-3 polyunsaturated fatty acids,fiber,vitamins,minerals,probiotics,and polyphenol-rich diet have significant impact on improvement of intestinal permeability marked by lower zonulin concentrations.Higher zonulin concentrations are found in those with overweight and obesity indicating that these population have increased IP.Most studies were conducted in adults and there are limited studies in children and adolescents.In addition,no studies have assessed diet quality to obtain a comprehensive picture on the complexities of diet in the population in relation to intestinal permeability.Conclusions:Diet and nutritional status are linked to zonulin concentrations,indicating a role in intestinal permeability.Further research should be conducted to investigate the relationship between diet quality,as measured by appropriate diet quality indices,and intestinal permeability in children,adolescents,and adults.
