This study aimed to increase bacterial growth and 5-aminolevulinic acid（ALA） biosynthesis of Rhodobacter sphaeroides in wastewater treatment through adding ferrous ion（ Fe2＋ ）. Results demonstrated that Fe2＋ eff...This study aimed to increase bacterial growth and 5-aminolevulinic acid（ALA） biosynthesis of Rhodobacter sphaeroides in wastewater treatment through adding ferrous ion（ Fe2＋ ）. Results demonstrated that Fe2＋ effectively enhanced the biomass production and ALA yield of R. sphaeroides. Moreover, the optimal Fe2＋ dosage was found to be 400 μmol/L, which was associated with the highest biomass of 4015.3 mg/L and maximum ALA yield of 15.9 mg/g-dry cell weight（mg/g-DCW）. Mechanism analysis revealed that Fe2＋ vastly improved Adenosine Triphosphate（ATP） production by up-regulating the nif gene expression, and increasing ATP enhanced the biomass and ALA yield by supplying energy for bacterial growth and ALA biosynthesis, respectively. Correlation analysis showed that the ALA and ATP yields had positive relation with nifA and nifU gene expression. In addition, the nifA and nifU gene expression displayed high consistency of co-transcription at the optimal Fe2＋ dosage.展开更多
This research focused on the three-dimensional(3 D) seepage field simulation of a high concrete-faced rockfill dam(CFRD) under complex hydraulic conditions. A generalized equivalent continuum model of fractured rock m...This research focused on the three-dimensional(3 D) seepage field simulation of a high concrete-faced rockfill dam(CFRD) under complex hydraulic conditions. A generalized equivalent continuum model of fractured rock mass was used for equivalent continuous seepage field analysis based on the improved node virtual flow method. Using a high CFRD as an example, the generalized equivalent continuum range was determined, and a finite element model was established based on the terrain and geological conditions, as well as structural face characteristics of the dam area. The equivalent seepage coefficients of different material zones or positions in the dam foundation were calculated with the Snow model or inverse analysis. Then, the 3 D seepage field in the dam area was calculated under the normal water storage conditions, and the corresponding water head distribution, seepage flow, seepage gradient, and seepage characteristics in the dam area were analyzed. The results show that the generalized equivalent continuum model can effectively simulate overall seepage patterns of the CFRD under complex hydraulic conditions and provide a reference for seepage analysis of similar CFRDs.展开更多
In order to explore the relationship between the macroscopic properties and pore structure characteristics of baking-free slag-sludge bricks,the compressive strength and water absorption rate using aluminum industrial...In order to explore the relationship between the macroscopic properties and pore structure characteristics of baking-free slag-sludge bricks,the compressive strength and water absorption rate using aluminum industrial slag and sludge of different substitution rates were tested.Optical instruments and image analysis software were used to measure the parameters of the pore structure.Specifically,a fractal model was built based on the fractal theory,in which the fractal dimension was used as the characteristic index of the pore structure to analyze the correlation and interaction mechanism between the fractal dimension and the macroscopic mechanical properties.Based on this study,conclusions were drawn as follows:(1)The plane pore structure of baking-free bricks shows good fractal characteristics.With the increase of the aluminum industrial slag content,the fractal dimension becomes smaller and the pore structure develops well;with the increase of the sludge content,the fractal dimension becomes larger and the pore structure becomes worse;(2)As the fractal dimension of the plane pore structure increases,the compressive strength of baking-free bricks falls,and the porosity and water absorption rise;(3)The optimum content of baking-free slag-sludge bricks is aluminum industrial slag 30%and sludge 10%.After 7 days of curing,baking-free bricks boast higher performance,and fully meet the performance requirements of solid concrete bricks(MU40),which can reduce time costs,and enhance enterprise revenue.展开更多
Taking an industrial sludge and its preparation of sludge wrap shell aggregates(WSAs)instead of sand to prepare baking-free brick as the research object,the development law of mechanical properties and the influence m...Taking an industrial sludge and its preparation of sludge wrap shell aggregates(WSAs)instead of sand to prepare baking-free brick as the research object,the development law of mechanical properties and the influence mechanism of macro and micro characteristic parameters of the bricks under different sludge and WSAs replacement rates were studied through the macroscopic mechanical properties test,with the help of nuclear magnetic resonance(NMR),transmission electron microscopy-energy spectrum and other testing technology and pores and cracks analysis system(PCAS)software.The results showed that the compressive strength of each sample decreased with the increase of sludge content.When the sludge content was less than 30%,it was mainly affected by the water-binder ratio.When the sludge content was more than 30%,it was mainly affected by the sludge content.At the age of 7 days,with the increase in replacement rate of WSAs,the compressive strength of the S10 and S30 groups was higher than that of the control group.The compressive strength of the S50 experimental group was 30.38 MPa,and the loss of compressive strength was slight compared with the control group.The water absorption rate of the 28 days S100 experimental group increased by 10.71%compared with the control group.When the content of WSAs was less than 50%,the holes above 0.1μm in the brick can be reduced and transformed into smaller holes,with a decreasing trend of the plane porosity of the brick.The microscopic results of the baking-free brick showed that the three-phase system of WSAs-interface transition area-mortar was poorly bonded and delaminated compared with the gravel aggregate-interface transition area-mortar system,and damage was more likely to occur in the WSAs and interface transition area.The above results show that it is feasible to use sludge and WSAs instead of sand for the preparation of baking-free bricks.This technology not only solves the problem of sludge disposal,but also protects the over-exploitation of mineral resources,and the technology has a broad application prospect and market value.展开更多
A recycled aggregate(RA)was prepared by crushing and sieving demolished discarded concrete pavements and was subsequently tested and analyzed to determine its various physical properties.On this basis,pervious concret...A recycled aggregate(RA)was prepared by crushing and sieving demolished discarded concrete pavements and was subsequently tested and analyzed to determine its various physical properties.On this basis,pervious concrete(PC)mix proportions were designed.Coarse RA particles with sizes of 5–10 and 10–20 mm were selected.Concrete specimens were prepared with a water–cement ratio of 0.3,an aggregate–cement ratio of 4.5,the substitute rates of RA with 0,25%,50%,75%and a single-/double-gap-graded RA mix(mass ratio of particles with sizes of 5–10 mm to particles with sizes of 10–20 mm:1:1,1:2,2:1,2:3 and 3:2).Various properties of the RA-containing PC(RPC)were determined by analyzing the compressive strength,splitting tensile strength,effective porosity,permeation coefficient and impact and abrasion resistance of the specimens.The results showed the following:The density of the RPC decreased with an increasing RA replacement ratio.The density of the RPC prepared with a double-gapgraded RA mix was lower than that prepared with a single-gap-graded RA(particle size:10–20 mm)mix.The permeation coefficient of the RPC increased with increasing porosity.The splitting tensile strength of the RPC was positively correlated with its compressive strength.The compressive strength of the RPC decreased with increasing porosity.The regression analysis showed that the impact and abrasion resistance of the RPC increased with increasing compressive strength.In addition,all of the RPC specimens met the strength and permeation requirements.This study can provide theoretical support for the application of RPC.展开更多
Based on the high sulfur content in titanium gypsum,the concept of the calcium-silicon-sulfur(Ca/Si/S)ratio was proposed.The Ca/Si/S ratio of concrete was adjusted by changing the titanium gypsum,fly ash,and cement co...Based on the high sulfur content in titanium gypsum,the concept of the calcium-silicon-sulfur(Ca/Si/S)ratio was proposed.The Ca/Si/S ratio of concrete was adjusted by changing the titanium gypsum,fly ash,and cement con-tent.The effects of different Ca/Si/S ratios on the mechanical properties,hydration products,and concrete micro-structure were investigated by nuclear magnetic resonance,uniaxial compression,and scanning electron microscopy.The result shows:(1)The compressive strength of concrete mixed with titanium gypsum increases first and then decreases with the Ca/Si/S ratio decrease.When the Ca/Si/S ratio is 1:0.85:0.10,the strength reaches the peak and is lower than the blank group.(2)The microstructure indicates the addition of titanium gypsum can effectively stimulate the activity of fly ash.Still,too much or too little titanium gypsum will hamper concrete strength development.(3)Titanium gypsum concrete’s nuclear magnetic resonance T2 spectrum has two characteristic peaks.With the Ca/Si/S ratio decreasing,the micropores in the concrete expand towards the macropores.The compressive strength is negatively correlated with the proportion of macropores and is positively correlated with the proportion of no-capillary pores.展开更多
The existing traditional dam seepage pressure prediction models have problems such as falling into local optimum.The sparrow search algorithm(SSA)was improved as ISSA using both methods of nonlinear Sine Cosine optimi...The existing traditional dam seepage pressure prediction models have problems such as falling into local optimum.The sparrow search algorithm(SSA)was improved as ISSA using both methods of nonlinear Sine Cosine optimization algorithm and adaptive producer and scrounger ratio.We combined the Bidirectional Long Short-Term Memory(BiLSTM)neural network model with ISSA to develop the ISSA-BiLSTM seepage pressure prediction model.The critical feature factors were extracted based on LightGBM to construct the input layer for seepage pressure prediction.The results show that the ISSA-BiLSTM model's fitting outcomes are generally consistent with the observed changes in seepage pressure observations,achieving an R^(2) of 0.987.In comparison to SSABiLSTM and BiLSTM,the model exhibits a substantial reduction in errors,decreasing by approximately 20%and 30%,respectively.This model can provide technical support and insights for accurately predicting dam seepage,contributing to the advancement of this field.展开更多
基金supported by the National Natural Science Foundation of China(No.51708214)the High-level Personnel Research Startup Project of North China University of Water Resources and Electric Power(No.40550)the Treatment Technology Integration and Demonstration for Domestic Sewage of Typical Villages and Towns in Henan Province(No.161100310700)
文摘This study aimed to increase bacterial growth and 5-aminolevulinic acid（ALA） biosynthesis of Rhodobacter sphaeroides in wastewater treatment through adding ferrous ion（ Fe2＋ ）. Results demonstrated that Fe2＋ effectively enhanced the biomass production and ALA yield of R. sphaeroides. Moreover, the optimal Fe2＋ dosage was found to be 400 μmol/L, which was associated with the highest biomass of 4015.3 mg/L and maximum ALA yield of 15.9 mg/g-dry cell weight（mg/g-DCW）. Mechanism analysis revealed that Fe2＋ vastly improved Adenosine Triphosphate（ATP） production by up-regulating the nif gene expression, and increasing ATP enhanced the biomass and ALA yield by supplying energy for bacterial growth and ALA biosynthesis, respectively. Correlation analysis showed that the ALA and ATP yields had positive relation with nifA and nifU gene expression. In addition, the nifA and nifU gene expression displayed high consistency of co-transcription at the optimal Fe2＋ dosage.
基金supported by the National Natural Science Youth Foundation of China(Grant No.51309101)the Henan Province Major Scientific and Technological Projects(Grant No.172102210372)the Cooperative Project of Production,Teaching and Research in Henan Province(Grant No.18210700031)
文摘This research focused on the three-dimensional(3 D) seepage field simulation of a high concrete-faced rockfill dam(CFRD) under complex hydraulic conditions. A generalized equivalent continuum model of fractured rock mass was used for equivalent continuous seepage field analysis based on the improved node virtual flow method. Using a high CFRD as an example, the generalized equivalent continuum range was determined, and a finite element model was established based on the terrain and geological conditions, as well as structural face characteristics of the dam area. The equivalent seepage coefficients of different material zones or positions in the dam foundation were calculated with the Snow model or inverse analysis. Then, the 3 D seepage field in the dam area was calculated under the normal water storage conditions, and the corresponding water head distribution, seepage flow, seepage gradient, and seepage characteristics in the dam area were analyzed. The results show that the generalized equivalent continuum model can effectively simulate overall seepage patterns of the CFRD under complex hydraulic conditions and provide a reference for seepage analysis of similar CFRDs.
基金Science and Technology Project of Henan Water Resources Department support program.No.GG202040Fund of Innovative Education Program for Graduate Students at North China University of Water Resources and Electric Power,China.No.YK2020-04.
文摘In order to explore the relationship between the macroscopic properties and pore structure characteristics of baking-free slag-sludge bricks,the compressive strength and water absorption rate using aluminum industrial slag and sludge of different substitution rates were tested.Optical instruments and image analysis software were used to measure the parameters of the pore structure.Specifically,a fractal model was built based on the fractal theory,in which the fractal dimension was used as the characteristic index of the pore structure to analyze the correlation and interaction mechanism between the fractal dimension and the macroscopic mechanical properties.Based on this study,conclusions were drawn as follows:(1)The plane pore structure of baking-free bricks shows good fractal characteristics.With the increase of the aluminum industrial slag content,the fractal dimension becomes smaller and the pore structure develops well;with the increase of the sludge content,the fractal dimension becomes larger and the pore structure becomes worse;(2)As the fractal dimension of the plane pore structure increases,the compressive strength of baking-free bricks falls,and the porosity and water absorption rise;(3)The optimum content of baking-free slag-sludge bricks is aluminum industrial slag 30%and sludge 10%.After 7 days of curing,baking-free bricks boast higher performance,and fully meet the performance requirements of solid concrete bricks(MU40),which can reduce time costs,and enhance enterprise revenue.
基金the Natural Science Foundation of Henan Province(202300410270)Graduate Education Innovation Program fund of North China University of Water Resources and Electric Power(YK-2021-49).
文摘Taking an industrial sludge and its preparation of sludge wrap shell aggregates(WSAs)instead of sand to prepare baking-free brick as the research object,the development law of mechanical properties and the influence mechanism of macro and micro characteristic parameters of the bricks under different sludge and WSAs replacement rates were studied through the macroscopic mechanical properties test,with the help of nuclear magnetic resonance(NMR),transmission electron microscopy-energy spectrum and other testing technology and pores and cracks analysis system(PCAS)software.The results showed that the compressive strength of each sample decreased with the increase of sludge content.When the sludge content was less than 30%,it was mainly affected by the water-binder ratio.When the sludge content was more than 30%,it was mainly affected by the sludge content.At the age of 7 days,with the increase in replacement rate of WSAs,the compressive strength of the S10 and S30 groups was higher than that of the control group.The compressive strength of the S50 experimental group was 30.38 MPa,and the loss of compressive strength was slight compared with the control group.The water absorption rate of the 28 days S100 experimental group increased by 10.71%compared with the control group.When the content of WSAs was less than 50%,the holes above 0.1μm in the brick can be reduced and transformed into smaller holes,with a decreasing trend of the plane porosity of the brick.The microscopic results of the baking-free brick showed that the three-phase system of WSAs-interface transition area-mortar was poorly bonded and delaminated compared with the gravel aggregate-interface transition area-mortar system,and damage was more likely to occur in the WSAs and interface transition area.The above results show that it is feasible to use sludge and WSAs instead of sand for the preparation of baking-free bricks.This technology not only solves the problem of sludge disposal,but also protects the over-exploitation of mineral resources,and the technology has a broad application prospect and market value.
基金This study was funded by the National key research and development program fund project(No.2018YFC0406803).
文摘A recycled aggregate(RA)was prepared by crushing and sieving demolished discarded concrete pavements and was subsequently tested and analyzed to determine its various physical properties.On this basis,pervious concrete(PC)mix proportions were designed.Coarse RA particles with sizes of 5–10 and 10–20 mm were selected.Concrete specimens were prepared with a water–cement ratio of 0.3,an aggregate–cement ratio of 4.5,the substitute rates of RA with 0,25%,50%,75%and a single-/double-gap-graded RA mix(mass ratio of particles with sizes of 5–10 mm to particles with sizes of 10–20 mm:1:1,1:2,2:1,2:3 and 3:2).Various properties of the RA-containing PC(RPC)were determined by analyzing the compressive strength,splitting tensile strength,effective porosity,permeation coefficient and impact and abrasion resistance of the specimens.The results showed the following:The density of the RPC decreased with an increasing RA replacement ratio.The density of the RPC prepared with a double-gapgraded RA mix was lower than that prepared with a single-gap-graded RA(particle size:10–20 mm)mix.The permeation coefficient of the RPC increased with increasing porosity.The splitting tensile strength of the RPC was positively correlated with its compressive strength.The compressive strength of the RPC decreased with increasing porosity.The regression analysis showed that the impact and abrasion resistance of the RPC increased with increasing compressive strength.In addition,all of the RPC specimens met the strength and permeation requirements.This study can provide theoretical support for the application of RPC.
基金National Natural Science Foundation of China(5210090341)Natural Science Foundation of Henan Province(202300410270)Fund of Innovative Education Program for Graduate Students at North China University of Water Resources and Electric Power,China(Grading No.YK-2021-39).
文摘Based on the high sulfur content in titanium gypsum,the concept of the calcium-silicon-sulfur(Ca/Si/S)ratio was proposed.The Ca/Si/S ratio of concrete was adjusted by changing the titanium gypsum,fly ash,and cement con-tent.The effects of different Ca/Si/S ratios on the mechanical properties,hydration products,and concrete micro-structure were investigated by nuclear magnetic resonance,uniaxial compression,and scanning electron microscopy.The result shows:(1)The compressive strength of concrete mixed with titanium gypsum increases first and then decreases with the Ca/Si/S ratio decrease.When the Ca/Si/S ratio is 1:0.85:0.10,the strength reaches the peak and is lower than the blank group.(2)The microstructure indicates the addition of titanium gypsum can effectively stimulate the activity of fly ash.Still,too much or too little titanium gypsum will hamper concrete strength development.(3)Titanium gypsum concrete’s nuclear magnetic resonance T2 spectrum has two characteristic peaks.With the Ca/Si/S ratio decreasing,the micropores in the concrete expand towards the macropores.The compressive strength is negatively correlated with the proportion of macropores and is positively correlated with the proportion of no-capillary pores.
基金supported by the North China University of Water Resources and Electric Powersupport of The National Natural Science Foundation of China(51979169)Henan Province Innovation Talent Support Plan(24HASTIT017).
文摘The existing traditional dam seepage pressure prediction models have problems such as falling into local optimum.The sparrow search algorithm(SSA)was improved as ISSA using both methods of nonlinear Sine Cosine optimization algorithm and adaptive producer and scrounger ratio.We combined the Bidirectional Long Short-Term Memory(BiLSTM)neural network model with ISSA to develop the ISSA-BiLSTM seepage pressure prediction model.The critical feature factors were extracted based on LightGBM to construct the input layer for seepage pressure prediction.The results show that the ISSA-BiLSTM model's fitting outcomes are generally consistent with the observed changes in seepage pressure observations,achieving an R^(2) of 0.987.In comparison to SSABiLSTM and BiLSTM,the model exhibits a substantial reduction in errors,decreasing by approximately 20%and 30%,respectively.This model can provide technical support and insights for accurately predicting dam seepage,contributing to the advancement of this field.
