Chaohu Lake, located in the central Anhui Province, is one of the five largest fresh lakes in China. Now it is one of the three most eutrophication lakes in China. The deterioration of its water quality has influenced...Chaohu Lake, located in the central Anhui Province, is one of the five largest fresh lakes in China. Now it is one of the three most eutrophication lakes in China. The deterioration of its water quality has influenced the sustainable development of society, economy and environment of Hefei City, the capital of Anhui Province. A series of measures have been carried out to control its eutrophication, but it is still serious. On the basis of the lake water quality data from 1984 to 2003, the causes of the eutrophication of Chaohu Lake are analyzed. Studies indicated that the suitable natural conditions and human activities played a crucial role in the process of the eutrophication of Chaohu Lake. A great amount of industrial, agricultural and domestic sewage discharged into the lake is the main cause of eutrophication in the lake. Land use, soil erosion and shoreline collapse destroyed the watershed eco-environment and the terrestrial ecosystem of Chaohu Lake. And the building of Yuxi Gate extends the sluggish of the nutritious substance and speeds up the process of the eutrophication. From the view of systematic engineering and watershed ecology, a series of the countermeasures have been put forward to control the eutrophication.展开更多
Carotenoids are essential components in tea quality, contributing to leaf color and aroma. However, little information about carotenoids in different tea cultivars and their biosynthesis regulation mechanism during le...Carotenoids are essential components in tea quality, contributing to leaf color and aroma. However, little information about carotenoids in different tea cultivars and their biosynthesis regulation mechanism during leaf development is known. Here we analyzed carotenoids by HPLC in the buds and leaves of 113 tea cultivars harvested on the same day. By profile clustering, carotenoids were divided into five groups. Same group cultivars displayed divergence in the total content of carotenoids but a similar molar ratio. To figure out the molecular mechanisms of this phenomenon, we further characterized all functional lycopene cyclases, which are the branch point of the carotenoid biosynthesis pathway. Two β-lycopene cyclases(CsLCYB1 and CsLCYB2) and one ε-lycopene cyclase(CsLCYE1) were cloned. Subcellular localization analysis showed that all cloned CsLCYs were localized in plastids. Enzyme activity assays in E. coli indicated both CsLCYBs catalyzed lycopene into β-carotene, and CsLCYE1 produced δ-carotene and ε-carotene. We found CsLCYB1 and CsLCYE1 predominantly expressed in leaf, while CsLCYB2was mainly expressed during flowering stages. Suppression by antisense oligonucleotides reduced CsLCYB1 and CsLCYE1 transcripts and led to reduction of both β,β-branch and β,ε-branch carotenoids in leaf. The expression levels of CsLCYB1 showed a significant positive correlation withβ,β-branch carotenoids in leaf. Our study provides carotenoid profiles of different tea cultivars, which can assist tea producers in selecting cultivars of interest. Meanwhile, we proposed the molecular mechanism of carotenoids reflecting the tenderness of tea plant leaf from a metabolic flux perspective, and suggested lycopene cyclase that could be applied to the breeding of tea varieties with different branch carotenoids.展开更多
基金U nderthe auspicesofthe cooperative projectbetw een G overnm entofChina and Japan (N o.JIC A G L:SCH EFY 98)
文摘Chaohu Lake, located in the central Anhui Province, is one of the five largest fresh lakes in China. Now it is one of the three most eutrophication lakes in China. The deterioration of its water quality has influenced the sustainable development of society, economy and environment of Hefei City, the capital of Anhui Province. A series of measures have been carried out to control its eutrophication, but it is still serious. On the basis of the lake water quality data from 1984 to 2003, the causes of the eutrophication of Chaohu Lake are analyzed. Studies indicated that the suitable natural conditions and human activities played a crucial role in the process of the eutrophication of Chaohu Lake. A great amount of industrial, agricultural and domestic sewage discharged into the lake is the main cause of eutrophication in the lake. Land use, soil erosion and shoreline collapse destroyed the watershed eco-environment and the terrestrial ecosystem of Chaohu Lake. And the building of Yuxi Gate extends the sluggish of the nutritious substance and speeds up the process of the eutrophication. From the view of systematic engineering and watershed ecology, a series of the countermeasures have been put forward to control the eutrophication.
基金supported by grants from the National Natural Science Foundation of China (Grant No.32102433)。
文摘Carotenoids are essential components in tea quality, contributing to leaf color and aroma. However, little information about carotenoids in different tea cultivars and their biosynthesis regulation mechanism during leaf development is known. Here we analyzed carotenoids by HPLC in the buds and leaves of 113 tea cultivars harvested on the same day. By profile clustering, carotenoids were divided into five groups. Same group cultivars displayed divergence in the total content of carotenoids but a similar molar ratio. To figure out the molecular mechanisms of this phenomenon, we further characterized all functional lycopene cyclases, which are the branch point of the carotenoid biosynthesis pathway. Two β-lycopene cyclases(CsLCYB1 and CsLCYB2) and one ε-lycopene cyclase(CsLCYE1) were cloned. Subcellular localization analysis showed that all cloned CsLCYs were localized in plastids. Enzyme activity assays in E. coli indicated both CsLCYBs catalyzed lycopene into β-carotene, and CsLCYE1 produced δ-carotene and ε-carotene. We found CsLCYB1 and CsLCYE1 predominantly expressed in leaf, while CsLCYB2was mainly expressed during flowering stages. Suppression by antisense oligonucleotides reduced CsLCYB1 and CsLCYE1 transcripts and led to reduction of both β,β-branch and β,ε-branch carotenoids in leaf. The expression levels of CsLCYB1 showed a significant positive correlation withβ,β-branch carotenoids in leaf. Our study provides carotenoid profiles of different tea cultivars, which can assist tea producers in selecting cultivars of interest. Meanwhile, we proposed the molecular mechanism of carotenoids reflecting the tenderness of tea plant leaf from a metabolic flux perspective, and suggested lycopene cyclase that could be applied to the breeding of tea varieties with different branch carotenoids.
