Objective:By observing the effect of moxibustion at Shenque(CV 8)on spleen immune function in rats with long-term exercise-induced fatigue,we explored the mechanism of relieving exercise-induced fatigue.Materials and ...Objective:By observing the effect of moxibustion at Shenque(CV 8)on spleen immune function in rats with long-term exercise-induced fatigue,we explored the mechanism of relieving exercise-induced fatigue.Materials and Methods:The male rats were divided into five groups:normal group(no intervention),control group(moxibustion at CV 8),untreated group(treadmill training),tail group(moxibustion at nonacupoint after treadmill training),and CV 8 group(moxibustion at CV 8 after treadmill training).We observed the general behavior of the rats,measured the levels of interleukin-1β(IL-1β),IL-18,IL-6,interferon-γ,tumor necrosis factor-α,and IL-10 in serum,and examined CD3+,CD4+,CD8+,nuclear factor kappa B(NF-κB)p65,NLRP3,caspase-1,IL-18,and IL-1βin spleen tissue.Hematoxylin and eosin staining was used to observe the spleen.Results:After 8 weeks of training,the results in the normal group and CV 8 group were significantly better than those in the untreated and tail groups.In the untreated group and tail group,body weight,spleen mass,spleen index,and serum IL-10 concentrations decreased significantly(P<0.05 or P<0.01),whereas high expression was observed in other parts of the serum and spleen tissue(P<0.01).The spleen corpuscle in the white pulp was reduced and necrotic without obvious germinal centers.Conclusions:Moxibustion at CV 8 could inhibit the NF-κB/NLRP3/caspase-1 inflammatory pathway to improve immunity and relieve exercise-induced fatigue.展开更多
基金supported by Science and Technology Support Project of Hebei Provincial Administration of Traditional Chinese Medicine,China(No.2022095)。
文摘Objective:By observing the effect of moxibustion at Shenque(CV 8)on spleen immune function in rats with long-term exercise-induced fatigue,we explored the mechanism of relieving exercise-induced fatigue.Materials and Methods:The male rats were divided into five groups:normal group(no intervention),control group(moxibustion at CV 8),untreated group(treadmill training),tail group(moxibustion at nonacupoint after treadmill training),and CV 8 group(moxibustion at CV 8 after treadmill training).We observed the general behavior of the rats,measured the levels of interleukin-1β(IL-1β),IL-18,IL-6,interferon-γ,tumor necrosis factor-α,and IL-10 in serum,and examined CD3+,CD4+,CD8+,nuclear factor kappa B(NF-κB)p65,NLRP3,caspase-1,IL-18,and IL-1βin spleen tissue.Hematoxylin and eosin staining was used to observe the spleen.Results:After 8 weeks of training,the results in the normal group and CV 8 group were significantly better than those in the untreated and tail groups.In the untreated group and tail group,body weight,spleen mass,spleen index,and serum IL-10 concentrations decreased significantly(P<0.05 or P<0.01),whereas high expression was observed in other parts of the serum and spleen tissue(P<0.01).The spleen corpuscle in the white pulp was reduced and necrotic without obvious germinal centers.Conclusions:Moxibustion at CV 8 could inhibit the NF-κB/NLRP3/caspase-1 inflammatory pathway to improve immunity and relieve exercise-induced fatigue.
