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Influence of vegetation restoration on matrix structure and erosion resistance of iron tailings sites in eastern Hebei, China 被引量:2
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作者 Anning Wang Qiuxian Huang +2 位作者 Xuehua Xu Xiaogang Li Yuling Li 《Journal of Forestry Research》 SCIE CAS CSCD 2020年第3期969-980,共12页
The effects of vegetation restoration on matrix structure and erosion resistance of iron tailings were studied at dump sites in Malanzhuang, Qian'an, Hebei province, China. The restoration process involved soil sp... The effects of vegetation restoration on matrix structure and erosion resistance of iron tailings were studied at dump sites in Malanzhuang, Qian'an, Hebei province, China. The restoration process involved soil spray sowing restoration model with Rhus typhina, soil and iron tailings admixture spray sowing restoration model with Amorpha fruticosa Linn. and six-hole brick restoration model with Pinus tabulaeformis Carriere.–Amorpha fruticosa Linn. mixed-forest, and direct restoration model with Hippophae rhamnoides and Sabina vulgaris. Results show that the composition and distribution of particles and aggregates were closely related to erosion resistance(P < 0.05), indicating that matrix structure of iron tailings play an important role in erosion resistance. The improvement in matrix structure of iron tailings by the different restoration models was in the order of R. typhina soil spray sowing > A. fruticosa soil and iron tailings admixture spray sowing > mixed-forest six-hole brick >H. rhamnoides direct restoration > S. vulgaris direct restoration. The R. typhina soil spray sowing restorationmodel resulted in the greatest improvement in matrix structure of iron tailings, increasing the clay(10.6%) and large particle aggregates(18.7%) contents significantly(P < 0.01). Simultaneously, particle population, grading conditions(Cu= 28.86, Cs= 1.65), and aggregate stability(6.02) were significantly improved. The A. fruticosa soil and iron tailings admixture spray sowing restoration model,which effectively improved particle distribution(Cu-= 8.51, Cs= 1.07), increased the number of large aggregates considerably(9.6%), thereby increasing aggregate stability(6.2). The six-hole brick model significantly increased the number of large aggregates(4.0%) and improved the stability of aggregates(6.2). H. rhamnoides direct restoration improved the stability of aggregates(5.1)but showed no other significant improvements. The effect of S. vulgaris direct restoration on matrix structure of iron tailings was not significant. Due to its dependence on matrix structure of iron tailings, the erosion resistance of R.typhina soil spray sowing restoration model was the greatest, while that of S. vulgaris direct restoration was the weakest. There was no significant difference in the erosion resistance of the other models. Overall, vegetation restoration supplemented by soil spray sowing restoration and engineering measures is superior to in situ direct vegetation restoration in the short-term. In-situ direct restoration has long-term ecological significance because of its advanced concept of near-natural restoration and the advantages of low cost, easy operation, and low secondary damage. 展开更多
关键词 EROSION resistance Matrix structure Iron TAILINGS VEGETATION RESTORATION
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Research on Rural Consumer Demand in Hebei Province Based on Principal Component Analysis 被引量:2
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作者 MA Hui-zi ZHAO Bang-hong XUAN Yong-sheng 《Asian Agricultural Research》 2011年第5期55-58,共4页
By selecting the time sequence data concerning influencing factors of rural consumer demand in Hebei Province from 2000 to 2010,this paper uses the principal component analysis method in multiplex econometric statisti... By selecting the time sequence data concerning influencing factors of rural consumer demand in Hebei Province from 2000 to 2010,this paper uses the principal component analysis method in multiplex econometric statistical analysis,constructs the principal component of consumer demand in Hebei Province,conducts regression on the dependent variable of consumer spending per capita in Hebei Province and the principal component of consumer demand so as to get principal component regression,and then conducts quantitative and qualitative analysis on the principal component.The results show that total output value per capita(yuan),employment rate,and income gap,are correlative with rural residents'consumer demand in Hebei Province positively;consumer price index,upbringing ratio of children,and one-year interest rate are correlative with rural residents'consumer demand in Hebei Province negatively;the ratio of supporting the elderly and medical care spending per capita are correlative with rural residents'consumer demand in Hebei Province positively.The corresponding countermeasures and suggestions are put forward to promote residents'consumer demand in Hebei Province as follows:develop county economy in Hebei Province and increase rural residents'consumer demand;use industry to support agriculture and coordinate urban-rural development;improve rural medical care and health system and resolve actual difficulties of the masses. 展开更多
关键词 Consumer demand Principal component analysis Regression analysis Hebei Province China
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Demonstration and Radiation Extension mode of Agricultural Technology——A Case of the Science and Technology Project of High Yield in Hebei Province 被引量:1
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作者 WANG Xiao-ying SHI Zheng +1 位作者 YANG Ying-guang ZHAO Hui-feng 《Asian Agricultural Research》 2010年第2期37-39,44,共4页
Firstly,relying on the science and technology project of high yield in Hebei Province,connotation of agricultural technology demonstration and extension mode and the high yield grain project in Hebei Province is intro... Firstly,relying on the science and technology project of high yield in Hebei Province,connotation of agricultural technology demonstration and extension mode and the high yield grain project in Hebei Province is introduced.Extension mode of agricultural technology demonstration and radiation is constructed.Agricultural technology demonstration and radiation mode includes the radiation center,primary irradiation and secondary irradiation.Secondly,management system and operational mechanism of agricultural technology demonstration and radiation mode are discussed,mainly expressed in establishing leading group in each demonstration county(city),carrying out leader contract responsibility system,establishing expert advisor steering group and setting up core experts group for subject,establishing technical experts group for subject,setting up leading group in the radiation area,carrying out chief expert responsibility system and technician matrix responsibility system,establishing the operating mechanism of "open,flow,competition and collaboration" with "test area-expert two-way selection" as the core content.Finally,countermeasures to improve the agricultural technology demonstration and radiation mode is put forward,such as establishing the rural technology demonstration base,strengthening cooperation with enterprises,and adopting flexible technical training,so as to promote the spread of agricultural high-tech,to increase the contribution rate of agricultural technology,and to offer ideas for agricultural technology extension model at the new era. 展开更多
关键词 Science and technology project of high yield Demonstration and radiation Agricultural extension mode China
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The Research of Impact of Returning Cultivated Land to Forests in Poverty Areas around Beijing-Tianjin on Local Economy——A Case of Shunping County in Hebei Province,China 被引量:1
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作者 ZHAO Li ZHANG Peng-tao 《Asian Agricultural Research》 2010年第12期20-22,27,共4页
Taking Shunping County of Hebei Province as an example,according to the relevant survey data in the year 2008 after returning cultivated land and data in the year 2001 before returning cultivated land,the thesis analy... Taking Shunping County of Hebei Province as an example,according to the relevant survey data in the year 2008 after returning cultivated land and data in the year 2001 before returning cultivated land,the thesis analyzes the impact of implementation of project of returning cultivated land to forests on local land use structure,three industrial added value and famers'per capita income.The results show that returning cultivated land has played a certain positive role on the local economic development and the improvement of people's life level.However,there are also some shortcomings:although famers'income is increasing with low growth rate,a large portion of famers still have not shaken off poverty;the farmers depends on subsidies greatly and the compensation mechanism of returning cultivated land is not considered well.China should further perfect the ecological compensation plan,and build rational ecological compensation mechanism in order to make the areas of returning cultivated land have self-blood-producing function;Shunping County should make full use of the location advantage in areas around Beijing-Tianjin,develop deep processing based on fruit trees industry,strengthen farmers'skill training,and increase input in the agricultural infrastructure construction to propel the sustainable development of agriculture. 展开更多
关键词 Areas around Beijing-Tianjin Economic poverty Returning cultivated to forests Shunping County China
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Empirical research on impacted factor of farmer's willingness for purchasing information Based on the survey data of Hebei province 被引量:1
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作者 SUN Gui-zhen LI Ya-qing 《Chinese Business Review》 2009年第10期58-63,共6页
Farmer's willingness for purchasing information, an important factor of farmer's information demand, was analyzed by logit model, according to the survey data of 535 farmers from eleven cities in Hebei province. The... Farmer's willingness for purchasing information, an important factor of farmer's information demand, was analyzed by logit model, according to the survey data of 535 farmers from eleven cities in Hebei province. The result showed that 6 factors had a significant influence on it, that is, education of farmers, per capita income of farmers, the affordability of information risk, the proportion of agricultural labor households, the type of agricultural production and the rural information service stations. On the basis of these, the related policy recommendations were proposed. 展开更多
关键词 FARMER willingness for purchasing information empirical analysis Hebei province
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Cloning and sequence analysis of E2 gene of bovine viral diarrhea virus HB-DCZ strain in Hebei province of China 被引量:1
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作者 ZHAO Yue-lan ZUO Yu-zhu +3 位作者 FAN Jing-hui ZHANG Lei QIN Jian-hua ZHANG Ning 《Journal of Agricultural Science and Technology》 2008年第10期6-11,16,共7页
The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus (BVDV) which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned... The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus (BVDV) which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned into pMD18-T vector, and then transfected Escherichia Coli JMI00. The recombinant plasmids were amplified by PCR and were sequenced. From the nucleotide sequence of the amplified products, phylogenetie analyses were performed and genotypes or subgenotypes were identified. The results indicated that the E2 gene fragment of HB-DCZ strain contained 1277bp nucleotides, and had 89.4%, 70.7%, 97.6%, 68.9%, 67.2% sequence similarity with Osloss, OregonC24V, Changchun184, ZM195, NADL, respectively. In conclusion, HB-DCZ strain is closely related to BVDV Osloss, Changchun184, and belongs to subgenotype lb. 展开更多
关键词 bovine viral diarrhea virus HB-DCZ virus strain E2 gene CLONING SEQUENCING PHYLOGENETICS GENOTYPES
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The Plights and Countermeasures in the Implementation of Distinctive Agriculture Insurance of Hebei Province
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作者 LING Mei WANG Wei-guo WANG Xue-yan 《Asian Agricultural Research》 2011年第3期65-69,共5页
The feasibility of implementing distinctive agriculture insurance in Hebei Province is analyzed from the following aspects covering the support of policies and laws;agricultural products and brands with Hebei distinct... The feasibility of implementing distinctive agriculture insurance in Hebei Province is analyzed from the following aspects covering the support of policies and laws;agricultural products and brands with Hebei distinct;the rich experience of insurance companies;farmers'strong awareness on insurance and farmers'great demand on agricultural insurance.The plights faced by distinctive agricultural insurance are pointed out.It includes weak awareness of farmers on buying insurance;low enthusiasm of insurance company;poor support from the government and backward laws and regulations and so on.The countermeasures on developing distinctive agricultural insurance are put forward.The countermeasures on improving farmers'awareness on buying insurance;innovating the insurance products and services;increasing the support from the government;perfecting the legislation on agricultural insurance should be adopted to develop agricultural insurance. 展开更多
关键词 Distinctive agricultural insurance Insurance awareness Product innovation Policy support LEGISLATION China
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Investigation and Solutions of Rural Micro Insurance——A Case of Hebei Province
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作者 WANG Wei-guo LING Mei LI Jia 《Asian Agricultural Research》 2010年第8期19-22,共4页
On the strength of investigation,the status quo of rural micro insurance of Hebei Province is introduced.The results show that it is characterized by the following features:rapid development;continuously increased exp... On the strength of investigation,the status quo of rural micro insurance of Hebei Province is introduced.The results show that it is characterized by the following features:rapid development;continuously increased experimental types and experimental areas;increasingly improved ability of serving the economic and social development;the combination of micro insurance and small loan has achieved success.Based on the efforts of analyzing the questionnaires,the following problems of the development of rural small loan are detected.For instance,the inadequate promotion on micro insurance;peasants'weak awareness of insurance;unclear understanding of the functions of micro insurance;unitary marketing channel of micro insurance;complex reasons of peasants'unwillingness to buy micro insurance and the poor service quality of insurance company.In view of these problems,countermeasures and suggestions are put forward,which include strengthening the promotion on micro insurance,improving peasants'awareness of insurance;expanding the marketing channel and innovating operation mode;innovating the products of micro insurance and encouraging technological innovation;improving the quality of service and establishing the excellent image of insurance company;strengthening the support from government and assisting peasants to insure. 展开更多
关键词 Rural micro insurance Marketing channel Management pattern Insurance products Hebei Province China
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Empirical Analysis of the Vegetable Industry in Hebei Province
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作者 LI Peng-hua ZHANG Run-qing LIU Yan 《Asian Agricultural Research》 2012年第4期30-33,36,共5页
We first introduce the status quo of the development of vegetable industry in Hebei Province,and then conduct empirical analysis of the development of vegetable industry in Hebei Province.Further,we analyze the develo... We first introduce the status quo of the development of vegetable industry in Hebei Province,and then conduct empirical analysis of the development of vegetable industry in Hebei Province.Further,we analyze the development advantage of the vegetable industry in Hebei Province using SAI(Scale Advantage Indices) and SCA(Symmetric Comparative Advantage),drawing the conclusion that the vegetable industry in Hebei Province has much room for development;at the same time,we analyze the factors influencing vegetable consumption of residents in Hebei Province through the regression model,drawing the conclusion that the vegetable consumer price index is the main factor affecting the consumption.Finally we make recommendations for the development of vegetable industry in Hebei Province as follows:increasing financial input,promoting policy guarantee capacity;implementing brand strategy,promoting the competitiveness of products;improving the ecological environment,promoting industrialization of pollution-free vegetables. 展开更多
关键词 The vegetable industry Scale advantage Indices Comparative advantage index Consumer price level Hebei Province
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Evaluation on Agricultural Regional Coordination Degree of Beijing,Tianjin and Hebei
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作者 HE Ling JIA Qi-jian WANG Jun 《Asian Agricultural Research》 2010年第10期5-8,共4页
According to the characteristics of agricultural system in Beijing,Tianjin and Hebei,population pressure,resource utilization and construction capability are taken as the first grade indices.And there are a total of 1... According to the characteristics of agricultural system in Beijing,Tianjin and Hebei,population pressure,resource utilization and construction capability are taken as the first grade indices.And there are a total of 14 second grade indices,such as the per capita cultivated land,per capita grain yield,per capita meat product,per capita aquatic product,per capita fruit,per capita water resource,per capita GDP,multiple cropping index,and proportion of agricultural expenditure in financial expenditure.An evaluation theory system for the coordination degree of agricultural system is established;and the empirical study on the agricultural coordination degree of Beijing,Tianjin and Hebei is carried out.Result shows that agricultural cooperative development in Beijing,Tianjin and Hebei is still in its primary stage.It is put forward that ideology is the premise,plan should take the lead,transportation is the foundation,and enhancing the brand is the key.Countermeasures are put forward for the agricultural cooperative development in Beijing,Tianjin and Hebei,such as enhancing the sense of strategic positioning during agricultural cooperative development in Beijing,Tianjin and Hebei,promoting the agricultural cooperative development planning in Beijing,Tianjin and Hebei,establishing a modern and integrated transport network system,strengthening the agricultural science and technology support,and improving the grade of agricultural products. 展开更多
关键词 Agricultural cooperation Coordination degree BEIJING Tianjin and Hebei China
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Empirical study on evaluation of regional agri-food logistics capability in e-commerce environment based on factor analysis A case study of Hebei province
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作者 YANG Lei ZHANG Yi-zhen 《Chinese Business Review》 2010年第12期38-43,共6页
With SPSS16.0 software, this paper chooses the statistical data of 11 cities in Hebei province to empirically study the agri-food logistics capability based on the method of factor analysis, and finally obtains the ra... With SPSS16.0 software, this paper chooses the statistical data of 11 cities in Hebei province to empirically study the agri-food logistics capability based on the method of factor analysis, and finally obtains the ranking of the 11 cities. It shows that, factor-cluster analysis is an effective method to analyze the logistics capability of agri-food. It can simplify the original complicated problem and lead to an objective, reliable and convincing conclusion. 展开更多
关键词 factor analysis E-COMMERCE agri-food logistics regional logistics capability
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Rabbit corneal damage threshold induced by a tunable laser system at 254 nm
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作者 Litong Zhu Zukang Nie +6 位作者 Mengqi Qiu Ying Wang Congling Zhou Lifei Guo Jiarui Wang Zaifu Yang Luguang Jiao 《Journal of Innovative Optical Health Sciences》 2025年第6期99-106,共8页
Low-pressure mercury lamps,with a main emission of about 254 nm,have been used for disinfecting air,water and surfaces for nearly a century.However,only a few studies on the corneal damage threshold at the wavelength ... Low-pressure mercury lamps,with a main emission of about 254 nm,have been used for disinfecting air,water and surfaces for nearly a century.However,only a few studies on the corneal damage threshold at the wavelength of 254nm exist.In this paper,the in vivo corneal damage threshold was determined inchinchilla rabbit model using a laser system at 254 nm.The irradiance of the laser spot was nearly flat-top distributed and the beam diameter on the animal corneal surface was about 3.44mm and 3.28 mm along the horizontal and vertical directions.Damage lesion determinations were performed at 12 h post-exposure using fluorescein sodium staining.TheED50 was17.7 mJ/cm^(2)with a 95%confidence interval of 15.3-20.1 mJ/cm^(2).The obtained results may contribute to the knowledge base for the refinement of the UV hazard function. 展开更多
关键词 Corneal damage threshold ultraviolet-C UV hazard function
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小麦遗传转化研究进展(英文) 被引量:2
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作者 Abdul Razzaq 马峙英 王海波 《分子植物育种》 CAS CSCD 2004年第4期457-464,共8页
Gradual progress made in genetic transformation of wheat is presented in this paper. Information on promoters, antibiotic, herbicide and auxotrophic markers, and various traits of wheat modified through genetic transf... Gradual progress made in genetic transformation of wheat is presented in this paper. Information on promoters, antibiotic, herbicide and auxotrophic markers, and various traits of wheat modified through genetic transformation, is provided. In addition the methods used for wheat transformation are discussed. Though significant efforts have been made for genetic transformation of wheat mainly through particle bombardment method but transformation efficiency is still low for mass production of fertile transgenic plants. Studies on the inheritance of transgenes and its incorporation into commercial elite cultivars are not significant. Agrobacterium mediated transformation seems to have better prospects for wheat transformation in future due to its advantages over particle bombardment. In planta transformation of wheat tissues seems possible only with Agrobacterium. 展开更多
关键词 小麦 遗传转化 抗生素 除草剂 营养水平
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Study on Supercooling Point and Freezing Point in Floral Organs of Apricot 被引量:15
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作者 MENG Qing-rui LIANG Yin-quan WANG Wen-feng DU Shao-hua LI Yan-hui YANG Jian-min 《Agricultural Sciences in China》 CAS CSCD 2007年第11期1330-1335,共6页
Under the environment of an artificial climate chamber, supercooling point (SCP) and freezing point (FP) in flower and young fruit at different development stages and freezing injuries of floral organs were studie... Under the environment of an artificial climate chamber, supercooling point (SCP) and freezing point (FP) in flower and young fruit at different development stages and freezing injuries of floral organs were studied. The apricot cultivars tested were Kety, Golden Sun and Honghebao. With the development of flower buds, SCP and FP increased, which indicated that their cold resistance decreased. SCP and FP varied with different floral organs. For different apricot cultivars, it was found that, the lower SCP or FP in floral organs was, the more resistant capacity the cultivar had, and the larger the temperature interval from SCP to FP was. SCP was not a constant value, but a range. Frequency distribution of SCP in petals was more dispersing than that in stamens and pistils. Floral organs could maintain a supercooling state to avoid ice formation, but they were sensitive to freezing. Once floral organs froze, thev turned brown after thawing. 展开更多
关键词 APRICOT floral organs supercooling point freezing point FROST
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Effects of cadium, zinc and lead on soil enzyme activities 被引量:29
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作者 YANG Zhi-xin LIU Shu-qing +1 位作者 ZHENG Da-wei FENG Sheng-dong 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2006年第6期1135-1141,共7页
Heavy metal (HM) is a major hazard to the soil-plant system. This study investigated the combined effects of cadium (Cd), zinc (Zn) and lead (Pb) on activities of four enzymes in soil, including calatase, urea... Heavy metal (HM) is a major hazard to the soil-plant system. This study investigated the combined effects of cadium (Cd), zinc (Zn) and lead (Pb) on activities of four enzymes in soil, including calatase, urease, invertase and alkalin phosphatase. HM content in tops of canola and four enzymes activities in soil were analyzed at two months after the metal additions to the soil. Pb was not significantly inhibitory than the other heavy metals for the four enzyme activities and was shown to have a protective role on calatase activity in the combined presence of Cd, Zn and Pb; whereas Cd significantly inhibited the four enzyme activities, and Zn only inhibited urease and calatase activities. The inhibiting effect of Cd and Zn on urease and calatase activities can be intensified significantly by the additions of Zn and Cd. There was a negative synergistic inhibitory effect of Cd and Zn on the two enzymes in the presence of Cd, Zn and Pb. The urease activity was inhibited more by the HM combinations than by the metals alone and reduced approximately 20%--40% of urease activity. The intertase and alkaline phosphatase activities significantly decreased only with the increase of Cd concentration in the soil. It was shown that urease was much more sensitive to HM than the other enzymes. There was a obvious negative correlation between the ionic impulsion of HM in soil, the ionic impulsion of HM in canola plants tops and urease activity. It is concluded that the soil urease activity may be a sensitive tool for assessing additive toxic combination effect on soil biochemical parameters. 展开更多
关键词 multiple heavy metal pollution canola plants soil enzyme ionic impulsion
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Effect of Phopshate Fertilizer and Manure on Crop Yield, Soil P Accumulation, and the Environmental Risk Assessment 被引量:16
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作者 LIU Jian-ling LIAO Wen-hua ZHANG Zuo-xin ZHANG Hai-tao WANG Xin-jun MENG Na 《Agricultural Sciences in China》 CAS CSCD 2007年第9期1107-1114,共8页
Phosphorus (P) applied from fertilizer and manure is important in increasing crop yield and soil fertility; however, excessive uses of phosphate fertilizer and manure may also increase P loss from agricultural soils... Phosphorus (P) applied from fertilizer and manure is important in increasing crop yield and soil fertility; however, excessive uses of phosphate fertilizer and manure may also increase P loss from agricultural soils, posing environmental impact. A long term experiment was conducted on a calcareous soil (meadow cinnamon) in Hebei Province, China, from 2003 to 2006 to investigate the effects of phosphate fertilizer and manure on the yield of Chinese cabbage, soil P accumulation, P sorption saturation, soluble P in runoff water, and P leaching. P fertilizer (P2O5) application at a rate of 360 kg ha^-1 or manure of 150 t ha^-1 significantly increased Chinese cabbage yield as compared to the unfertilized control. However, no significant yield response was found with excessive phosphate or manure application. Soil Olsen-P, soluble P, bioavailable P, the degree of phosphorus sorption saturation in top soil layer (0-20 cm), and soluble P in runoff water increased significantly with the increase of phosphate fertilizer and manure application rates, whereas the maximum phosphorus sorption capacity (Qm) decreased with the phosphate fertilizer and manure application rates. Soil Olsen-P and soluble P also increased significantly in the sub soil layer (20-40 cm) with the high P fertilizer and manure rates. It indicates that excessive P application over crop demand can lead to a high environmental risk owing to the enrichment of soil Olsen-P, soluble P, bioavailable P, and the degree of phosphorus sorption saturation in agricultural soils. 展开更多
关键词 phosphate fertilizer MANURE yield response P accumulation in soil environmental risk of P loss
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Functional Analysis of MYB73 of Arabidopsis thaliana Against Bipolaris oryzae 被引量:16
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作者 JIA Jiao XING Ji-hong DONG Jin-gao HAN Jian-min LIU Jun-sheng 《Agricultural Sciences in China》 CAS CSCD 2011年第5期721-727,共7页
Bipolaris oryzae is a severe rice disease,resulting in significant economic losses.MYB73 gene of Arabidopsis was isolated based on T-DNA insertion mutants whose lose-of-function mutant increased susceptibility to B.or... Bipolaris oryzae is a severe rice disease,resulting in significant economic losses.MYB73 gene of Arabidopsis was isolated based on T-DNA insertion mutants whose lose-of-function mutant increased susceptibility to B.oryzae.Results of staining for H2O2 revealed that infection of incompatible B.oryzae caused much more strong brown patches on the leaves of myb73 mutant than those on the leaves of wild type.Expression of MYB73 gene was induced by B.oryzae.Its increased expression was severely impaired in the myb73 mutant.Expression of MYB73 was severely decreased in the npr1,jar1,eds5,and sid2 mutants,suggesting MYB73 gene participates in the jasmonate (JA) and salicylic acid (SA) signaling pathways.Expression of MYB73 who encoded an R2R3 MYB transcription factor was increased by SA,JA,and ethylene (ET) treatments.The cDNA full-length sequence of MYB73 was 960 bp and a protein with 320 amino acids was encoded.The predicted molecular weight of MYB73 was 34.85 kDa.The expression of MYB73 gene was induced within 24 h of the inoculation,however,the expression of PR1,PDF1.2 and NPR1 weren't changed.When B.oryzae successfully infected myb73 mutant plants,the expression of PR1,PDF1.2 and NPR1 were increased.Collectively,our results suggested that MYB73 is involved in NPR1-mediated SA and JA signaling pathways. 展开更多
关键词 ARABIDOPSIS MYB73 RT-PCR Bipolaris oryzae
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Novel method for the determination of five carbamate pesticides in water samples by dispersive liquid-liquid microextraction combined with high performance liquid chromatography 被引量:10
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作者 Zhi Mei Liu Xiao Huan Zang Wei Hua Liu Chun Wang Zhi Wang 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第2期213-216,共4页
A novel method for the determination of five carbamate pesticides (metolcarb, carbofuran, carbaryl, isoprocard and diethofencard) in water samples was developed by dispersive liquid-liquid microextraction (DLLME) ... A novel method for the determination of five carbamate pesticides (metolcarb, carbofuran, carbaryl, isoprocard and diethofencard) in water samples was developed by dispersive liquid-liquid microextraction (DLLME) coupled with high performance liquid chromatography-diode array detector (HPLC-DAD). Some experimental parameters that influence the extraction efficiency were studied and optimized to obtain the best extraction results. Under the optimum conditions for the method, the calibration curve was linear in the concentration range from 5 to 1000 ng mL^-1 for all the five carbamate pesticides, with the correlation coefficients (r^2) varying from 0.9984 to 0.9994. Good enrichment factors were achieved ranging from 80 to 177- fold, depending on the compound. The limits of detection (LODs) (S/N = 3) were ranged from 0.1 to 0.5 ng mL^-1. The method has been successfully applied to the analysis of the pesticide residues in environmental water samples. 展开更多
关键词 Carbamate pesticides High performance liquid chromatography Diode array detection Dispersive liquid-liquid microextraction Water samples
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Molecular Characterization and Expression Analysis of TaZFP15, a C_2H_2-Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.) 被引量:24
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作者 SUN Zhao-hua DING Chang-huan +1 位作者 LI Xiao-juan XIAO Kai 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第1期31-42,共12页
Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag (EST) sharing high similarity to a rice C2H2 zinc finger transcription factor (ZFP15) was obtained in wheat. Through... Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag (EST) sharing high similarity to a rice C2H2 zinc finger transcription factor (ZFP15) was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 (GenBank accession no. AY286473), maize ZFP (GenBank accession no. NM_001159094) and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid (ABA). TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses. 展开更多
关键词 Triticum aestivum L. zinc-finger transcription factor gene nutrition deficiency abiotic stress expression pattern
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