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Nicastrin、N1ICD及hes1蛋白在小鼠肝脏及肝癌组织中的表达
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作者 严璐 史天威 《实用医学杂志》 CAS 北大核心 2024年第6期768-772,共5页
目的 检测C57BL/6小鼠正常肝脏和肝癌组织中nicastrin、N1ICD和hes1蛋白的表达。方法 将12只6周龄雄性C57BL/6小鼠随机分为对照组和模型组,每组6只。模型组小鼠注射Hepa1-6肝癌细胞构建小鼠原位肝癌模型,对照组注射等量的生理盐水。HE... 目的 检测C57BL/6小鼠正常肝脏和肝癌组织中nicastrin、N1ICD和hes1蛋白的表达。方法 将12只6周龄雄性C57BL/6小鼠随机分为对照组和模型组,每组6只。模型组小鼠注射Hepa1-6肝癌细胞构建小鼠原位肝癌模型,对照组注射等量的生理盐水。HE染色观察肝脏癌变情况。IHC与Western blot检测nicastrin、N1ICD和hes1蛋白在正常肝脏组织及肝癌组织中的表达情况。结果 IHC显示,nicastrin、N1ICD及hes1蛋白在正常肝细胞中均定位于肝窦内皮细胞,肝细胞基本不表达;相比对照组肝脏组织,在模型组小鼠肝癌组织中,nicastrin蛋白在肝癌细胞中表达增多(P <0.01),N1ICD及hes1蛋白表达均减少(P <0.05,P <0.01)。结论 NCSTN基因在小鼠肝细胞癌中发挥促癌作用;notch1与hes1在小鼠肝细胞癌中可能发挥抑癌作用。 展开更多
关键词 NCSTN notch1受体 hes1蛋白 肝细胞癌 hepa1-6 小鼠
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狐肺炎大肠杆菌yihe基因缺失株的构建及致病性 被引量:2
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作者 张志强 苏硕青 +4 位作者 杜万年 李永慧 吴同垒 史秋梅 朱国强 《中国兽医学报》 CAS CSCD 北大核心 2019年第8期1495-1499,共5页
为研究YihE蛋白在狐肺炎大肠杆菌致病过程中的作用,利用λ-Red同源重组方法构建狐源大肠杆菌yihe基因缺失株,并从生长特性、生化特性、抗吞噬能力、菌株毒力等几方面对突变菌株进行评价。结果表明:与狐肺炎大肠杆菌野生型菌株相比,yihe... 为研究YihE蛋白在狐肺炎大肠杆菌致病过程中的作用,利用λ-Red同源重组方法构建狐源大肠杆菌yihe基因缺失株,并从生长特性、生化特性、抗吞噬能力、菌株毒力等几方面对突变菌株进行评价。结果表明:与狐肺炎大肠杆菌野生型菌株相比,yihe基因缺失株生长特性和生化特性无明显差异,但其抗吞噬细胞吞噬能力以及胞内存活能力有明显下降,细菌半数致死量有显著提高。本试验成功构建狐肺炎大肠杆菌yihe基因缺失株,为研究YihE蛋白的作用机制奠定基础。 展开更多
关键词 狐肺炎大肠杆菌 yihe基因 基因缺失 YihE 致病性
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A case report of traditional Chinese medicine in the treatment of Huhuo disease
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作者 LI Na LENG Junyan +1 位作者 HE Jun'an ZHANG Hong 《World Journal of Integrated Traditional and Western Medicine》 2024年第2期72-76,共5页
Objective: To cultivate the clinical thinking of traditional Chinese medicine(TCM).Methods: A 53-year-old male patient with fever, systemic rash accompanied by oral, perineal and perianal ulcers for 5 days was enrolle... Objective: To cultivate the clinical thinking of traditional Chinese medicine(TCM).Methods: A 53-year-old male patient with fever, systemic rash accompanied by oral, perineal and perianal ulcers for 5 days was enrolled and managed utilizing traditional Chinese medicine principles.Results: By enhancing relevant auxiliary examinations to exclude hand-foot-and-mouth disease, combined with the four diagnostic methods of traditional Chinese medicine, the diagnosis was established as Huhuo disease, and the differentiation was dampness-heat encumbering inside. On the basis of modern medical fluid rehydration and nutritional support(glucocorticoids, immunosuppressants and other drugs were not used in the whole treatment).The patient was treated with Chinese medicine differentiation, and administered Gancao Xiexin decoction for 9 days and then was recovered and discharged.Conclusions: Based on the trinity of “theory-classic-clinical”, the cultivation of TCM thinking can be realized. 展开更多
关键词 Case report Traditional Chinese herbal medicine Gancao Xiexin decoction
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老年COPD并呼吸衰竭血清CHE表达检测及其与动脉血PaO2的相关性研究
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作者 刘欣 高婧 杨美菊 《罕少疾病杂志》 2024年第7期35-36,39,共3页
目的分析老年慢性阻塞性肺疾病(COPD)并呼吸衰竭患者血清胆碱酯酶(CHE)表达检测及其与动脉血动脉血氧分压(PaO2)的相关性。方法选取2021年6月~2023年6月收治的100例COPD老年患者为COPD组,以是否合并呼吸衰竭为依据,将COPD组分为合并组(... 目的分析老年慢性阻塞性肺疾病(COPD)并呼吸衰竭患者血清胆碱酯酶(CHE)表达检测及其与动脉血动脉血氧分压(PaO2)的相关性。方法选取2021年6月~2023年6月收治的100例COPD老年患者为COPD组,以是否合并呼吸衰竭为依据,将COPD组分为合并组(合并呼吸衰竭)与未合并组(未合并呼吸衰竭),分别为61例、39例。随访时间截止为患者出院时间,以随访结局是否为死亡为依据,将合并组分为存活组(44例)与死亡组(17例);以病情程度为依据,将合并组分为急性加重组(11例)与稳定组(50例),同期选取50例健康体检者为对照组。回顾性分析入组者临床资料,记录年龄、性别、病程、治疗情况、血气分析、病情程度、CHE水平及随访结局,对比对照组、合并组与未合并组、稳定组与急性加重组、死亡组与存活组PaO2、CHE水平,以Pearson法分析合并组患者CHE与PaO2的相关性。结果合并组与未合并组PaO2、CHE水平较对照组更低(P<0.05);合并组PaO2、CHE水平较未合并组更低(P<0.05);急性加重组PaO2、CHE水平较稳定组更低(P<0.05);死亡组PaO2、CHE水平较存活组更低(P<0.05);Pearson分析显示,合并组血清CHE水平与PaO2呈正相关(r=0.533,P<0.05)。结论CHE在老年COPD并呼吸衰竭患者血清中低表达,与PaO2呈正相关,检测CHE可准确反映患者病情程度,并可用于预后评估。 展开更多
关键词 慢性阻塞性肺疾病 动脉血氧分压 呼吸衰竭 相关性 胆碱酯酶
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HE4在上皮性卵巢癌中的表达情况及其与临床特征及预后的关系分析 被引量:16
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作者 党秋红 曹静 +2 位作者 张欢欢 曾宪旭 靳耀锋 《癌症进展》 2018年第5期629-631,635,共4页
目的探讨HE4在上皮性卵巢癌中的表达情况及其与临床特征及预后的关系。方法选取150例上皮性卵巢癌患者和72例良性卵巢疾病患者,采用酶联免疫吸附法(ELISA)检测并比较150例上皮性卵巢癌患者和72例良性卵巢疾病患者的血清HE4水平;采用免... 目的探讨HE4在上皮性卵巢癌中的表达情况及其与临床特征及预后的关系。方法选取150例上皮性卵巢癌患者和72例良性卵巢疾病患者,采用酶联免疫吸附法(ELISA)检测并比较150例上皮性卵巢癌患者和72例良性卵巢疾病患者的血清HE4水平;采用免疫组织化学法(IHC)检测150例上皮性卵巢癌患者癌组织和相应癌旁正常组织中HE4的表达情况,分析其与上皮性卵巢癌临床特征的关系;随访36个月,记录并分析HE4表达与上皮性卵巢癌患者生存情况的关系。结果上皮性卵巢癌患者的血清HE4水平明显高于良性卵巢疾病患者,上皮性卵巢癌组织中HE4蛋白的阳性表达率明显高于癌旁正常组织,差异均有统计学意义(P﹤0.01);肿瘤直径﹥3 cm、低分化、Ⅲ~Ⅳ期、有淋巴结转移的上皮性卵巢癌组织的HE4蛋白的阳性表达率分别高于肿瘤直径≤3 cm、中高分化、Ⅰ~Ⅱ期、无淋巴结转移的癌组织,差异均有统计学意义(P﹤0.05);随访36个月,HE4蛋白阳性表达的上皮性卵巢癌患者的生存率低于阴性表达的患者,差异有统计学意义(P﹤0.05)。结论 HE4是上皮性卵巢癌的良好肿瘤标志物,与肿瘤的良、恶性鉴别及临床特征有密切的关系,对上皮性卵巢癌患者的早期诊断及预后有一定的价值。 展开更多
关键词 上皮性卵巢癌 HE4 临床特征 预后
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非酒精性脂肪性肝病患者血清Chemerin和颈动脉内膜中层厚度的相关性
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作者 高海丽 杨道坤 +3 位作者 梁海军 王新伟 王燕平 陈宝鑫 《肝脏》 2021年第4期429-434,共6页
目的探讨非酒精性脂肪性肝病(NAFLD)患者血清Chemerin和颈动脉内膜中层厚度(CIMT)的相关性。方法回顾性纳入2019年1月1日至2020年5月1日于新乡医学院第一附属医院就诊的96例NAFLD患者和体检中心35例健康体检者,其中NAFLD患者CIMT≥1.0 m... 目的探讨非酒精性脂肪性肝病(NAFLD)患者血清Chemerin和颈动脉内膜中层厚度(CIMT)的相关性。方法回顾性纳入2019年1月1日至2020年5月1日于新乡医学院第一附属医院就诊的96例NAFLD患者和体检中心35例健康体检者,其中NAFLD患者CIMT≥1.0 mm定义为ACIMT组,CIMT<1.0 mm定义为NCIMT组,健康体检者定义为对照组。比较三组研究对象性别、年龄、BMI、吸烟史、合并糖尿病病史、高血压病史、收缩压、舒张压、FBG、TC、TG、HDL-c、LDL-c、HbA1c、FPG、FINS、HOMA-IR、ALT、AST、UA、Chemerin和CIMT的差异,相关性分析采用Pearson分析,NAFLD患者IMT的危险因素采用Logistic回归分析,血清Chemerin水平诊断NAFLD患者IMT≥1.0 mm的临床效能采用ROC曲线评估。结果TG、LDL-c、FINS、HOMA-IR、ALT、AST、Chemerin在对照组、NCIMT组和ACIMT组中依次升高,组组比较均存在显著差异(P<0.05);FPG、UA和CIMT在对照组和NCIMT组中相比较均无显著差异(P>0.05);与对照组和NCIMT组相比较,ACIMT组中FPG、UA和CIMT均显著升高(P<0.05)。NAFLD患者CIMT水平与TG、LDL-c、HOMA-IR、UA和Chemerin均呈现显著正相关性,相关系数分别为0.322、0.474、0.354、0.365和0.489。Logistic回归分析显示,TG(OR=4.221)、HOMA-IR(OR=1.353)、LDL-c(OR=5.548)和Chemerin(OR=8.015)是NAFLD患者CIMT的危险因素。血清中Chemerin表达水平诊断NAFLD患者CIMT≥1.0 mm的曲线下面积为0.885(95%CI:0.813~0.942,P<0.000),当截断值为265.29 ng/mL时,诊断敏感度和特异度分别为93.24%和95.25%。结论Chemerin是NAFLD患者CIMT增厚的一项危险因素,其可作为临床上检测NAFLD患者颈动脉粥样硬化的一项生物学标志物。 展开更多
关键词 非酒精性脂肪性肝病 颈动脉内膜中层厚度 CHEMERIN 标志物
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UPLC-MS/MS法测定人血浆替代基质中8-HETE浓度的不确定度评定
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作者 慕杨娜 邸子真 +6 位作者 赵磊 杨瑞 唐思 刘君 滕丹 于珊珊 李国信 《实用中医内科杂志》 2021年第12期14-17,共4页
目的评定UPLC-MS/MS法测定人血浆替代基质中8-HETE浓度的不确定度。方法借助数学模型分析不确定度的来源,确定影响因素。以A类评定程序评价分析过程中随机效应引起的不确定度,以B类评定程序评价分析过程的其他因素引起的不确定度,包括:... 目的评定UPLC-MS/MS法测定人血浆替代基质中8-HETE浓度的不确定度。方法借助数学模型分析不确定度的来源,确定影响因素。以A类评定程序评价分析过程中随机效应引起的不确定度,以B类评定程序评价分析过程的其他因素引起的不确定度,包括:称量、标准品纯度、量瓶允差、移液器及移液管允差、回收率及标准曲线的拟合等,计算合成不确定度及扩展不确定度。结果置信概率P为95%时,人血浆替代基质中低(2.06μg/L)、中(25.28μg/L)、高(80.72μg/L)浓度8-HETE的扩展不确定度分别为1.44μg/L、3.08μg/L及8.78μg/L。结论本方法绿色环保,样品回收率高,数据准确、可靠,适用于UPLC-MS/MS法测定人血浆替代基质中8-HETE浓度,测量不确定度主要由线性回归过程引入,其他分量影响较小。该方法能够满足检测标准的要求,确保数据的国际互认。 展开更多
关键词 8-HETE UPLC-MS/MS法 人血浆替代基质 不确定度 扩展不确定度
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苏鲁造山带东段新生代两阶段剥露事件的磷灰石(U-Th)/He热年代学证据 被引量:7
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作者 林旭 吴林 +2 位作者 Marc Jolivet 李长安 刘海金 《地球科学》 EI CAS CSCD 北大核心 2022年第4期1162-1176,共15页
苏鲁造山带位于华北和华南板块之间,是中国东部最显著的陆内造山带之一,约束其新生代剥露过程对于理解中国东部盆山格局分布及其动力学机制具有重要意义.低温热年代学方法由于封闭温度较低,能更准确地约束上地壳地质体的剥露过程.利用... 苏鲁造山带位于华北和华南板块之间,是中国东部最显著的陆内造山带之一,约束其新生代剥露过程对于理解中国东部盆山格局分布及其动力学机制具有重要意义.低温热年代学方法由于封闭温度较低,能更准确地约束上地壳地质体的剥露过程.利用磷灰石(U-Th)/He方法,对苏鲁造山带东部的多福山和锯齿山开展研究.磷灰石(U-Th)/He年龄-高程和热历史模拟结果显示,多福山和锯齿山在早-中始新世(54~43 Ma)和渐新世(35~27 Ma)发生剥露.这与苏鲁造山带西段的剥露时间同步.结合区域内已报道的研究结果,表明中国东部造山带受太平洋板块、印度板块向欧亚大陆俯冲的影响,在早新生代出现广泛的阶段性剥露过程,从而奠定了中国东部的盆山分布格局. 展开更多
关键词 苏鲁造山带 磷灰石 隆升 (U-Th)/He热年代学 构造
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HE4、OPN、MSLN水平对上皮性卵巢癌的诊断价值 被引量:6
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作者 赵玉芝 蔡大军 +1 位作者 李元昆 张霞 《癌症进展》 2018年第3期331-333,共3页
目的探讨血清人附睾分泌蛋白4(HE4)、骨桥蛋白(OPN)及间皮素(MSLN)水平对上皮性卵巢癌(EOC)的诊断价值。方法选择100例EOC患者(EOC组)、60例卵巢上皮良性肿瘤患者(良性组)和60例健康女性体检者(健康组),检测3组研究对象的血清HE4、OPN及... 目的探讨血清人附睾分泌蛋白4(HE4)、骨桥蛋白(OPN)及间皮素(MSLN)水平对上皮性卵巢癌(EOC)的诊断价值。方法选择100例EOC患者(EOC组)、60例卵巢上皮良性肿瘤患者(良性组)和60例健康女性体检者(健康组),检测3组研究对象的血清HE4、OPN及MSLN水平,分析3种指标单独及联合诊断EOC的临床价值。结果 EOC组患者的血清HE4、OPN、MSLN水平均高于良性组和健康组,差异均有统计学意义(P﹤0.05);良性组和健康组的血清HE4、OPN、MSLN水平比较,差异均无统计学意义(P﹥0.05);血清OPN+HE4+MSLN正确诊断EOC患者92例,诊断灵敏度为92.00%,特异度为90.83%,漏诊率为8.00%,误诊率为9.17%,曲线下面积(AUC)为0.916。结论血清OPN+HE4+MSLN联合检测对EOC具有较高的诊断价值,较单一指标检测提高了灵敏度和特异度。 展开更多
关键词 人附睾分泌蛋白4 骨桥蛋白 间皮素 上皮性卵巢癌
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血清HE4、CA125联合检测对卵巢上皮性恶性肿瘤的诊断价值及其与临床特征的关系 被引量:5
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作者 孙晓 牛东升 +1 位作者 徐恩松 董阳 《癌症进展》 2018年第8期1016-1019,共4页
目的探讨血清人附睾分泌蛋白4(HE4)、糖类抗原125(CA125)联合检测对卵巢上皮性恶性肿瘤的临床诊断价值及其与患者临床特征的关系。方法选取60例卵巢上皮性恶性肿瘤患者(恶性组)和120例卵巢良性病变患者(良性组)作为研究对象,检测两组患... 目的探讨血清人附睾分泌蛋白4(HE4)、糖类抗原125(CA125)联合检测对卵巢上皮性恶性肿瘤的临床诊断价值及其与患者临床特征的关系。方法选取60例卵巢上皮性恶性肿瘤患者(恶性组)和120例卵巢良性病变患者(良性组)作为研究对象,检测两组患者的血清HE4、CA125水平,分析血清HE4、CA125水平与卵巢上皮性恶性肿瘤病理类型、FIGO分期及分化程度的关系,探究两项指标单独及联合检测对卵巢上皮性恶性肿瘤的诊断价值。结果恶性组患者的血清HE4、CA125水平分别为(161.3±48.9)pmol/L、(59.3±29.8)U/ml,均明显高于良性组患者的(87.3±26.1)pmol/L、(18.4±9.0)U/ml(P﹤0.01)。血清HE4单独诊断卵巢上皮性恶性肿瘤的灵敏度为63.3%,特异度为50.0%;CA125单独诊断卵巢上皮性恶性肿瘤的灵敏度为53.3%,特异度为45.8%;血清HE4+CA125联合诊断卵巢上皮性恶性肿瘤的灵敏度为91.7%,特异度为62.5%。血清HE4的阳性表达水平与卵巢上皮性恶性肿瘤患者的FIGO分期和分化程度有关(P﹤0.05),与病理类型无关(P﹥0.05);血清CA125的阳性表达水平与卵巢上皮性恶性肿瘤患者的病理类型、FIGO分期和分化程度均有关(P﹤0.05)。结论血清HE4、CA125联合检测对卵巢上皮性恶性肿瘤具有较高的诊断价值,较单一指标检测提高了灵敏度和特异度,值得临床推广应用。 展开更多
关键词 人附睾分泌蛋白4 糖类抗原125 卵巢上皮性恶性肿瘤 卵巢良性病变 临床特征
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Factors Affecting Adherence to National Malaria Treatment Guidelines in the Diagnosis, Treatment and Prevention of Malaria in Pregnancy among Healthcare Workers in Public Health Facilities in Jowhar District, Somalia
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作者 Abdullahi Muse Mohamoud Magda Elhadi Ahmed Yousif Osman Khalafalla Saeed 《Health》 CAS 2022年第11期1114-1129,共16页
Introduction: Early diagnosis and effective treatment remain critical elements of a malaria control strategy in preventing mortality and reducing the incidence of severe malaria illness in Somalia. In Sub-Saharan Afri... Introduction: Early diagnosis and effective treatment remain critical elements of a malaria control strategy in preventing mortality and reducing the incidence of severe malaria illness in Somalia. In Sub-Saharan African countries showed different levels of adherence to their national malaria guidelines for malaria treatment and prevention in pregnancy, while experiences from many countries indicated several challenges and constraints that may make the implementation of the guidelines difficult. Objective: The study aimed to assess factors affecting adherence to revised national malaria treatment guidelines in the diagnosis, treatment and prevention of malaria in pregnancy among health care workers in public health facilities in Jowhar District, Somalia. Methodology: A cross-sectional health facility-based study conducted in Jowhar district. A structured interview questionnaire was used for data collection from (n = 150). Healthcare workers selected from ten public health facilities using proportional to size sampling and an observational checklist was used to assess patient’s medical prescriptions to review their conformity to the guidelines and the availability of antimalarial drug, malaria diagnostic tests (mRDTs and microscopy) and job aids, such as the national malaria treatment guidelines, clinical algorithm (flow chart), malaria rapid diagnosis tests mRDTs’ use of wall charts, and drug dose wall charts at the facilities. Data was analyzed through the application of descriptive statistical analysis that includes frequency, and percentages by using Statistical Package for Social Sciences (SPSS) Version 25. Results: Healthcare workers interviewed were 89 (59.3%) aware of the existence of the revised national malaria treatment guidelines. However, 61 (40.7%) were not aware of the guidelines and only 46 (30.7%) had been trained for the national malaria guidelines in the treatment and prevention of malaria in pregnancy. Overall, 33 (22%) of the workers reported to adhere to guidelines, with 117 (78.0%) reported non-adherence. A significant difference was observed in the training status among different categories of health workers with (p-value of 0.022). Conclusion and Recommendation: In conclusion, the study showed that adherence to the national malaria guidelines for Malaria diagnosis, treatment and prevention in pregnancy among health care workers are associated with inadequate awareness of the revised national malaria guidelines among healthcare workers, inadequate supply of diagnostic tests (mRDTs and microscopy) at the health facilities and lack of access to revised national guidelines for malaria diagnosis and treatment, a lack of regular supervision and monitoring and lack of in-service training respectively. The national malaria control program should intensify efforts to strengthen the readiness of the public health facilities in the district to handle malaria in pregnancy cases, diagnosis, treatment and prevention, improve the availability of antimalarial drugs and malaria diagnostic tests (mRDTs and microscopy), job-aids at the health facilities and undertake regular monitoring and on job training to ensure the proper use of the guidelines at all levels of health care service delivery points across the country. 展开更多
关键词 MALARIA GUIDELINE ADHERENCE Malaria in Pregnancy Jowhar District SOMALIA
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Effect of In-Service Training Program on the Practice of Healthcare Workers toward Malaria Prevention and Treatment Guidelines during Pregnancy in Health Facilities in Jowhar District, Somalia
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作者 Abdullahi Muse Mohamoud Magda Elhadi Ahmed Yousif Osman Khalafalla Saeed 《Health》 CAS 2022年第11期1173-1190,共18页
Malaria is generally considered a major public health problem in Somalia. Providing early diagnosis and effective treatment is the key element of malaria control strategies in malaria-endemic countries, including Soma... Malaria is generally considered a major public health problem in Somalia. Providing early diagnosis and effective treatment is the key element of malaria control strategies in malaria-endemic countries, including Somalia. This required to advocate and ensure health worker’s adherence to the national malaria guidelines at all levels of health care service. A well-designed in-service training program may improve the level of health worker’s adherence to national malaria treatment guidelines, although results have been inconsistent. This is an interventional health facility-based pre and post comparative study aimed to assess the effect of an in-service training program on the practice of healthcare workers toward malaria prevention and treatment guidelines, during in pregnancy in health facilities in Jowhar district, Middle Shabelle region of Somalia. The study was implemented in three phases: pre-intervention phase, intervention phase and post-intervention phase. The sample size consisted of (n = 150) health workers who were selected from ten public health facilities using proportional to size sampling;the data collection adopted in this research is composed of a structured interview questionnaire and observational checklist. Data was analyzed through the application of descriptive statistical analysis that includes frequency and percentage and the Chi-square (x<sup>2</sup>) test was used to test the associations among variables using SPSS software version 25. The study showed that the level of health workers’ awareness of the national malaria guidelines in the treatment and prevention of malaria in pregnancy was found to be good before the intervention 89 (59.3%) and this proportion increased to 150 (100%) post-intervention of the training program. A significance difference has been observed between health workers’ awareness and their adherence to the malarial guidelines at pre-test and post-test with a p-value 0.000. The proportion of health workers who attended previous training on national malaria guidelines in the treatment and prevention of malaria in pregnancy increased from 46 (30.7%) at the pre-test to 150 (100%) after the post-test. A significant difference was observed in the training status among different categories of health worker and their adherence to the guidelines during the pre- and post-intervention of the training program, with a p-value of 0.000. The result showed that health workers were adhering to the guidelines at the pre-test 33 (22%), this increased after the post-test to 87 (58%). The knowledge of the need to adhere led to an increase in the adherence rate after the training program intervention. The study reveals that inadequate awareness was most reason for the non-adherence in the majority of the health workers as indicated by 89 (59.3%) at the pre-test and 56 (37.3%) in the post-test. However, difference was not significant between the availability of anti-malaria drugs in the facilities and the health workers’ adherence to the guidelines p-value 0.355 at the pretest and p-value 0.258 at post-test. The study concluded that the in-service training program significantly improved health workers’ knowledge and practice to the national malaria guidelines in the treatment, and prevention of malaria in pregnancy. The researcher recommends that the national malaria control programme (NMCP) of the Federal Ministry of Health should provide continuous regular in-service training to frontline healthcare workers at (facility and Community-based) to upgrade their skills and knowledge towards the malaria guidelines, disseminate job aids to the health facilities and undertake regular monitoring to ensure effective implementation of the national malaria treatment guidelines in the treatment and prevention of malaria in pregnancy in achieving desired proper case-management practices of malaria in pregnancy at all levels of health care service. 展开更多
关键词 Malaria In Pregnancy In-Service Training Healthcare Workers National Malaria Guideline Jowhar District SOMALIA
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Construction of 3D flowers-like O-doped g-C_(3)N_(4)-[N-doped Nb2O5/C]heterostructure with direct S-scheme charge transport and highly improved visible-light-driven photocatalytic efficiency 被引量:5
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作者 Fahim A.Qaraah Samah A.Mahyoub +3 位作者 Abdo Hezam Amjad Qaraah Qasem A.Drmosh Guangli Xiu 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 2022年第10期2637-2651,共15页
Constructing a suitable heterojunction photocatalytic system from two photocatalytic materials is an efficient approach for designing extremely efficient photocatalysts for a broader range of environmental,medical,and... Constructing a suitable heterojunction photocatalytic system from two photocatalytic materials is an efficient approach for designing extremely efficient photocatalysts for a broader range of environmental,medical,and energy applications.Recently,the construction of a step-scheme heterostructure system(hereafter called the S-scheme)has received widespread attention in the photocatalytic field due to its ability to achieve efficient photogenerated carrier separation and obtain strong photo-redox ability.Herein,a novel S-scheme heterojunction system consisting of 2D O-doped g-C_(3)N_(4)(OCN)nanosheets and 3D N-doped Nb_(2)O_(5)/C(N-NBO/C)nanoflowers is constructed via ultrasonication and vigorous agitation technique followed by heat treatment for the photocatalytic degradation of Rhodamine B(RhB).Detailed characterization and decomposition behaviour of RhB showed that the fabricated material shows excellent photocatalytic efficiency and stability towards RhB photodegradation under visible-light illumination.The enhanced performance could be attributed to the following factors:fast charge transfer,highly-efficient charge separation,extended lifetime of photoinduced charge carriers,and the high redox capability of the photoinduced charges in the S-scheme system.Various trapping experiment conditions and electron paramagnetic resonance provide clear evidence of the S-scheme photogenerated charge transfer path,meanwhile,the RhB mineralization degradation pathway was also investigated using LC-MS.This study presents an approach to constructing Nb_(2)O_(5)-based S-scheme heterojunctions for photocatalytic applications. 展开更多
关键词 2D/3D nanostructure S-scheme heterojunction g-C_(3)N_(4) Nb_(2)O_(5) Photocatalytic degradation
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2015 ATA指南超声模式在Bethesda Ⅲ类甲状腺结节中的应用价值 被引量:3
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作者 张利英 李月华 +3 位作者 温德惠 李晓娟 刘翔宇 薛刚 《现代肿瘤医学》 CAS 2018年第17期2689-2693,共5页
目的:探讨2015ATA指南超声模式对意义不明确的非典型病变(atypia of undetermined significance,AUS)和滤泡性病变(follicular lesion of undetermined significance,FLUS)的诊断价值。方法:选取最初经细针穿刺细胞学(fine needle aspir... 目的:探讨2015ATA指南超声模式对意义不明确的非典型病变(atypia of undetermined significance,AUS)和滤泡性病变(follicular lesion of undetermined significance,FLUS)的诊断价值。方法:选取最初经细针穿刺细胞学(fine needle aspiration,FNA)诊断为AUS/FLUS的204例甲状腺结节作为研究对象,分析并比较AUS及FLUS良恶性结节的临床资料及二维超声特征,对所有结节的二维超声图像行2015ATA指南超声模式分级标准进行分级,计算得出极低度、低度、中度及高度可疑恶性结节的恶性风险,使用受试者工作特征(ROC)曲线计算得出2015ATA超声模型鉴别AUS良恶性的最佳诊断界点,并根据诊断界点得出诊断效能等指标。结果:在AUS/FLUS良恶性结节之间,患者的年龄、性别及结节的大小差异无统计学意义(P>0.05);144例AUS中,恶性甲状腺结节多表现为不规则边界和微钙化且具有统计学意义(P=0.007,P=0.005);2015ATA指南超声模式对AUS类结节的良恶性评估有统计学意义(P=0.001),其极低度、低度、中度、高度可疑恶性结节的恶性率依次为0%、7.0%、64.3%、77.1%,恶性率随着分级的增高而增高,而在FLUS类结节良恶性鉴别诊断中差异无统计学意义;2015ATA对AUS诊断的敏感性、特异性、准确性、阳性预测值、阴性预测值及曲线下面积分别为87.8%、87.4%、87.5%、94.7%、73.5%、0.90。结论:2015ATA指南有助于鉴别AUS的良恶性,将Bethesda Ⅲ类甲状腺结节细分为AUS和FLUS两类,在临床工作中,有助于对Bethesda Ⅲ类甲状腺结节更好的管理及治疗。 展开更多
关键词 甲状腺细针穿刺细胞学 2015年美国甲状腺学会指南 意义不明确的非典型病变或滤泡性病变
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HER2阳性胃癌研究进展 被引量:8
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作者 孙克然 吕慧芳 +1 位作者 王赛琪 陈小兵 《中国医药导报》 CAS 2020年第30期33-36,58,共5页
目前为止,胃癌依然是全球第五大恶性肿瘤,也是全球第二大癌症相关死亡原因。前期的研究发现,表皮生长因子受体2(HER2)阳性胃癌是一种特殊类型的胃癌,预后更差,这种胃癌在发生早期的上皮内瘤变组织中即存在HER2的高表达,且随着癌前病变... 目前为止,胃癌依然是全球第五大恶性肿瘤,也是全球第二大癌症相关死亡原因。前期的研究发现,表皮生长因子受体2(HER2)阳性胃癌是一种特殊类型的胃癌,预后更差,这种胃癌在发生早期的上皮内瘤变组织中即存在HER2的高表达,且随着癌前病变去分化程度的升高,HER2的过表达率也显著增加,随着赫赛汀等靶向药物的出现HER2阳性胃癌的治疗问题出现了转机,但仅仅的赫赛汀治疗远远不够。HER2阳性胃癌的检查与治疗都还处于初级水平,近年来出现的液体活检技术以及多种治疗药物正在一步一步揭开HER2阳性胃癌神秘的面纱。其中液体活检技术不仅在效率上远超传统的免疫组化技术,而且可以实时动态监测曲妥珠单抗的疗效。本文综述了HER2阳性胃癌近阶段的研究进展,包括对于HER2阳性胃癌的治疗、耐药以及与免疫状态的关系,从而为临床实践提供一定的参考。 展开更多
关键词 胃癌 表皮生长因子受体2 曲妥珠单抗 程序性死亡配体1 吡咯替尼
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新辅助化疗联合中间性肿瘤细胞减灭术治疗晚期上皮性卵巢癌的疗效及对HE4、VEGF、CA125水平的影响 被引量:25
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作者 侯晓茹 郭田田 秦峰 《癌症进展》 2018年第6期722-725,共4页
目的探讨新辅助化疗联合中间性肿瘤细胞减灭术治疗晚期上皮性卵巢癌的疗效及其对血清人附睾蛋白4(HE4)、血管内皮细胞生长因子(VEGF)、糖类抗原125(CA125)水平的影响。方法回顾性分析68例晚期上皮性卵巢癌患者的病历资料,根据治疗方法... 目的探讨新辅助化疗联合中间性肿瘤细胞减灭术治疗晚期上皮性卵巢癌的疗效及其对血清人附睾蛋白4(HE4)、血管内皮细胞生长因子(VEGF)、糖类抗原125(CA125)水平的影响。方法回顾性分析68例晚期上皮性卵巢癌患者的病历资料,根据治疗方法不同将患者分为联合组与对照组,每组34例。联合组患者采用新辅助化疗联合中间性肿瘤细胞减灭术治疗,对照组患者仅接受卵巢肿瘤细胞减灭术治疗。观察并比较两组患者的近期疗效,手术相关指标,血清HE4、VEGF、CA125水平及预后情况。结果联合组患者的治疗总有效率为79.4%,高于对照组的52.9%(P﹤0.05);联合组患者的手术时间明显短于对照组(P﹤0.01);联合组患者的术中失血量、腹腔积液量均明显低于对照组(P﹤0.01);治疗后,联合组患者的血清HE4、VEGF、CA125水平均明显低于对照组(P﹤0.01);治疗后,两组患者的1年、2年、3年生存率比较,差异均无统计学意义(P﹥0.05)。联合组和对照组患者的中位生存时间分别为32.0个月(95%CI:25.3~35.6)和28.0个月(95%CI:22.1~33.9),两组患者的生存情况比较,差异无统计学意义(χ2=1.96,P﹥0.05)。结论新辅助化疗联合中间性肿瘤细胞减灭术治疗晚期上皮性卵巢癌可以缩短手术时间,减少术中出血量,降低血清肿瘤相关标志物水平,但该方法对改善患者的远期生存意义不大。 展开更多
关键词 新辅助化疗 中间性肿瘤细胞减灭术 人附睾蛋白4 血管内皮细胞生长因子 糖类抗原125 上皮性卵巢癌
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Development of signal analysis method for the motional Stark effect diagnostic on EAST 被引量:2
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作者 Jia FU Bo LYU +7 位作者 Haiqing LIU Yingying LI Dongmei LIU Yongqing WEI Chao FAN Yuejiang SHI Zhenwei WU Baonian WAN 《Plasma Science and Technology》 SCIE EI CAS CSCD 2017年第10期1-4,共4页
A pilot single-channel Motional Stark Effect(MSE) diagnostic has been developed on EAST since 2015. The dual photo-elastic modulators(PEM) were employed to encode the polarization angle into a time-varying signal.... A pilot single-channel Motional Stark Effect(MSE) diagnostic has been developed on EAST since 2015. The dual photo-elastic modulators(PEM) were employed to encode the polarization angle into a time-varying signal. The pitch angle was related to the ratio of modulation amplitude at the second harmonic frequency. A digital harmonic analyzer(DHA) technique was developed for extracting the second harmonic amplitude. The results were validated with a hardware phase lock-in amplifier, and is also consistent with the software dual phase-locking algorithm. 展开更多
关键词 motional Stark effect fast Fourier transform
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MySQL 中CASE-WHEN 的应用
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作者 刘磊 林丽丹 《电脑知识与技术》 2020年第35期228-229,共2页
实际研发工作中经常需要写各种SQL来统计线上的各种业务数据或者需要以查询的字段作为条件进行数据过滤操作,使用CASE-WHEN能让统计事半功倍,用好CASE-WHEN,解决的问题更广泛,逻辑上更为紧凑。文章首先介绍了业务需求,接着给出了实现方... 实际研发工作中经常需要写各种SQL来统计线上的各种业务数据或者需要以查询的字段作为条件进行数据过滤操作,使用CASE-WHEN能让统计事半功倍,用好CASE-WHEN,解决的问题更广泛,逻辑上更为紧凑。文章首先介绍了业务需求,接着给出了实现方案,然后提供了实例代码,最后总结了应用场景。 展开更多
关键词 MYSQL CASE 问卷调查 应用场景
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The Epidemiology of Induction of Labor among Women Aged 15 - 49 Who Delivered at Shaafi Hospital in Hodon District, Mogadishu Somalia 2020
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作者 Abdullahi Muse Mohamoud Sahra Mire Mohamed +4 位作者 Ahmed Mohamud Hussein Nafisa Ali Hassan Ruweyda Abdi Hassan Juweyriya Osman Abdullahi Naima Abdi Hashi 《Health》 CAS 2022年第4期418-431,共14页
Background: Induction of labor is an artificial termination utilized to decrease both maternal and neonatal morbidity and mortality. The study was aimed to assess the epidemiology of induction of labor among women age... Background: Induction of labor is an artificial termination utilized to decrease both maternal and neonatal morbidity and mortality. The study was aimed to assess the epidemiology of induction of labor among women aged 15 - 49 who delivered at Shaafi Hospital in Hodon District, Mogadishu Somalia 2020. Methodology: A cross-sectional and retrospective hospital-based study was conducted among 30 women aged 15 - 49 who had undergone induction of labor who were delivered at Shaafi Hospital Mogadishu during the period of the study from May-July 2020. Data were collected by interview method using structured questionnaire. Data analyzed was performed using Statistical Package for Social Science version 20. Results: The study showed that the majority of respondents 12 (40%) had undergone induction once time previously, followed by 8 (27%) had undergone induction previously two times, while 6 (20%) had undergone previously three times, 4 (13%) more than four times. 21 (70%) were delivered Spontaneous Vagina delivery (SVD), 6 (20%) were Cesarean section (CS) and 3 (10%) instrumental vaginal delivery. A total of (60%) were delivered in public hospitals, followed by (23%) were delivered in private hospitals and 5 (17%) home delivery. In addition the reasons of induction of labor a total of 27 (90%) were post term, followed by 2 (7%) were Intrauterine fetal death (IUFD), while few proportion of 1 (3%) were Pregnancy induced hypertension (PIH). Finally, the study revealed that the socio-demographic and obstetrical determinants such as age and daily meal intake as well as antenatal care visit and reasons for induction of labor e.g. Post term (Post-mature), Intrauterine fetal death (IUFD) and Pregnancy induced hypertension (PIH) showed significant association with their the induced labor (P Conclusion and Recommendations: The study recommended that ministry of health especially maternal and reproductive health unit should ensure that every pregnant woman has access to skilled maternal counseling and improve quality of antenatal, develop a national guideline of induction labor and continue training health workers at health facilities and monitor its implementations across the country. 展开更多
关键词 INDUCTION LABOR Shafi Hospital Mogadishu Somalia
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Design and Availability Research of a Flammable and Explosive Volatiles Monitoring and Early Warning System (FEVMEW) for the Bus Crowded Places
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作者 Cen Yu Weibin Guo +2 位作者 Ruyi Li Qiang Chen Tianping Xu 《Journal of Computer and Communications》 2020年第12期83-90,共8页
<div style="text-align:justify;"> In order to reduce the arson or accidental fire losses, we developed a gas sensitive detector used for the rapid detection and early warning of flammables in crowded p... <div style="text-align:justify;"> In order to reduce the arson or accidental fire losses, we developed a gas sensitive detector used for the rapid detection and early warning of flammables in crowded places such as buses. A MEMS (Micro-Electro-Mechanical System) based thin film semiconductor was fabricated as the gas sensor. To obtain the target gas selective response, the surface of the sensitive film was modified with highly active metal catalytic nano-particles. Thus the anti-interference ability was improved and the false alarm rate was effectively reduced. Furthermore, the modular embedded system for information acquisition and transmission was developed. Supported by the Airflow Precision control system (APs), the rapid warning of volatile gas of flammable substances was realized. Experiments showed that RAs has satisfied selectivity to volatiles of usual flammable liquid, such as the output voltage reaches 3 V (0 - 3.3 V). With simulation about the actual installation state in bus, MWs sounds an alarm at 2 minutes after splashing 50 mL 92# petrol to the floor. For the last two years, FEVMEW has been integrated into more than 4000 buses in Hefei. This design has been proved feasible according to the actual operation. </div> 展开更多
关键词 Flammable and Explosive Volatiles Monitoring and Early Warning Bus Crowded Places Gas Sensitive MEMS Chip Airflow Regulating
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