Discrimination among grapevine varieties based on quantitative traits,such as flowering,veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs.These trai...Discrimination among grapevine varieties based on quantitative traits,such as flowering,veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs.These traits are under complex genetic control for which 6 linked SSR loci(VVS2,VVIn16,VMC7G3,VrZAG29,VMC5G7,and VVIB23)have been identified.Using these markers in HRM-PCR analysis,we assessed genetic diversity among a large collection of 192 grapevine varieties.The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties,3 prominent foreign varieties and 11 varieties of Palestinian origin.The SSR markers used were highly polymorphic,displaying unique melting curves for unusually higher number of samples than generally observed in SSR analysis.This prompted us to examine sequence composition for selected samples and found that variation present as SNPs in the flanking sequences of SSR motifs was responsible for the observed polymorphism.Hence,HRM-PCR proved to be a tool of higher analytical power to distinguish genotypes surpassing the discrimination power of conventional gel-based SSR analysis.The study provides a better understanding of genetic variation of SSR marker loci associated to phenological traits in grapevine varieties,signifying an analytical methodology that may be of higher discrimination power in detection of polymorphism for utilization in breeding programs.展开更多
Soil is an integral component of the global environmental system which supports the quality and diversity of terrestrial life on Earth.Therefore,it is vital to consider the processes and impacts of soil degradation on...Soil is an integral component of the global environmental system which supports the quality and diversity of terrestrial life on Earth.Therefore,it is vital to consider the processes and impacts of soil degradation on society,especially on the provision of environmental goods and services,including food security and climate change mitigation and adaptation.Scientific societies devoted to soil science play significant roles in reducing soil degradation and promoting soil conservation by advancing scientificknowledge,education and environmental sustainability.The ESSC was founded on 4 November 1988,with the aims to:1.Support research on soil degradation,soil protection and soil and water conservation.2.Provide a network for the exchange of knowledge about soil degradation processes and soil conservation research and practises.3.Produce publications on major issues relating to soil degradation and soil and water conservation.4.Advise regulators and policy-makers on soil issues,especially soil degradation,protection and conservation.The societal challenges that can be addressed through better soil protection,advancing knowledge and scientific approaches to soil protection and sustainable management,mean the ESSC embraces the on-going development,application,review and constructive criticism of highly innovative scientific soil conservation methods,In this context,the ESSC analyses and publicizes the roles and functions of soil in natural and human-modified systems and the functional optimization of soils to ensure sustainable environmental protection.'The thin layer of soil that forms a patchy covering over the continents controls our awn existence and that of every other animal of the land' (Rachel Carson (1962) in'Silent Spring).展开更多
文摘Discrimination among grapevine varieties based on quantitative traits,such as flowering,veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs.These traits are under complex genetic control for which 6 linked SSR loci(VVS2,VVIn16,VMC7G3,VrZAG29,VMC5G7,and VVIB23)have been identified.Using these markers in HRM-PCR analysis,we assessed genetic diversity among a large collection of 192 grapevine varieties.The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties,3 prominent foreign varieties and 11 varieties of Palestinian origin.The SSR markers used were highly polymorphic,displaying unique melting curves for unusually higher number of samples than generally observed in SSR analysis.This prompted us to examine sequence composition for selected samples and found that variation present as SNPs in the flanking sequences of SSR motifs was responsible for the observed polymorphism.Hence,HRM-PCR proved to be a tool of higher analytical power to distinguish genotypes surpassing the discrimination power of conventional gel-based SSR analysis.The study provides a better understanding of genetic variation of SSR marker loci associated to phenological traits in grapevine varieties,signifying an analytical methodology that may be of higher discrimination power in detection of polymorphism for utilization in breeding programs.
文摘Soil is an integral component of the global environmental system which supports the quality and diversity of terrestrial life on Earth.Therefore,it is vital to consider the processes and impacts of soil degradation on society,especially on the provision of environmental goods and services,including food security and climate change mitigation and adaptation.Scientific societies devoted to soil science play significant roles in reducing soil degradation and promoting soil conservation by advancing scientificknowledge,education and environmental sustainability.The ESSC was founded on 4 November 1988,with the aims to:1.Support research on soil degradation,soil protection and soil and water conservation.2.Provide a network for the exchange of knowledge about soil degradation processes and soil conservation research and practises.3.Produce publications on major issues relating to soil degradation and soil and water conservation.4.Advise regulators and policy-makers on soil issues,especially soil degradation,protection and conservation.The societal challenges that can be addressed through better soil protection,advancing knowledge and scientific approaches to soil protection and sustainable management,mean the ESSC embraces the on-going development,application,review and constructive criticism of highly innovative scientific soil conservation methods,In this context,the ESSC analyses and publicizes the roles and functions of soil in natural and human-modified systems and the functional optimization of soils to ensure sustainable environmental protection.'The thin layer of soil that forms a patchy covering over the continents controls our awn existence and that of every other animal of the land' (Rachel Carson (1962) in'Silent Spring).
