Immunotherapies have demonstrated notable clinical benefits in the treatment of cervical cancer(CC).However,the development of therapeutic resistance and diverse adverse effects in immunotherapy stem from complex inte...Immunotherapies have demonstrated notable clinical benefits in the treatment of cervical cancer(CC).However,the development of therapeutic resistance and diverse adverse effects in immunotherapy stem from complex interactions among biological processes and factors within the tumor immune microenvironment(TIME).Advanced omic technologies offer novel insights into a more expansive and thorough layer of the TIME.Furthermore,integrating multidimensional omics within the frameworks of systems biology and computational methodologies facilitates the generation of interpretable data outputs to characterize the clinical and biological trajectories of tumor behavior.In this review,we present advanced omics technologies that utilize various clinical samples to address scientific inquiries related to immunotherapies for CC,highlighting their utility in identifying metastasis dissemination,recurrence risk,and therapeutic resistance in patients treated with immunotherapeutic approaches.This review elaborates on the strategy for integrating multi-omics data through artificial intelligence algorithms.Additionally,an analysis of the obstacles encountered in the multi-omics analysis process and potential avenues for future research in this domain are presented.展开更多
Non-right-handedness(NRH),encompassing left-handedness and mixed-handedness,has been frequently reported at elevated rates in individuals with various psychiatric disorders.The consistency of this association across m...Non-right-handedness(NRH),encompassing left-handedness and mixed-handedness,has been frequently reported at elevated rates in individuals with various psychiatric disorders.The consistency of this association across multiple conditions and its underlying mechanisms is the subject of ongoing investigation.This review synthesized current evidence to explore the association between NRH and psychiatric disorders from epidemiological,genetic,and neurobiological perspectives.We systematically identified and appraised relevant literature investigating NRH prevalence in psychiatric populations and potential explanatory mechanisms.Epidemiological evidence indicates an elevated prevalence of NRH,particularly within neurodevelopmental disorders.Potential contributing mechanisms identified include early developmental disruptions,shared genetic predispositions,and atypical patterns of brain lateralization.While the association between NRH and psychiatric conditions,especially neurodevelopmental disorders,is evident,the causal pathways and relative contributions of identified mechanisms are complex and debated.This review highlighted key areas requiring further research to elucidate these relationships.展开更多
BACKGROUND The liver represents a common site of distant metastasis in patients with esophageal cancer(EC).Conventional chemotherapy(CMT)presents limited efficacy for EC,and EC patients with liver metastases typically...BACKGROUND The liver represents a common site of distant metastasis in patients with esophageal cancer(EC).Conventional chemotherapy(CMT)presents limited efficacy for EC,and EC patients with liver metastases typically experience a poor prognosis,highlighting an urgent need to explore novel treatment approaches.This study evaluated the overall efficacy and safety of CMT vs CMT combined with immune checkpoint inhibitors(ICIs)in the treatment of EC patients with liver metastases.Furthermore,prognostic factors influencing outcomes in this patient population were identified.AIM To evaluate the efficacy and safety of first-line chemoimmunotherapy for EC patients with liver metastases and to analyze prognostic factors.METHODS This retrospective study included 126 EC patients with liver metastases at Zhejiang Cancer Hospital between 2014 and 2024.Patients receiving CMT were compared with those receiving CMT+ICI.Analyzed variables included clinicopathological features,treatment history,characteristics of metastasis,systemic and local treatments,overall survival(OS),and treatment-related adverse events(TRAEs).Prognostic factors were evaluated using univariate and multivariate Cox proportional-hazards regression models.Finally,efficacy outcomes and TRAE profiles were compared between the two groups.RESULTS A significant difference in median OS was identified between the two groups(10.8 months in the CMT group vs 20.8 months in the CMT+ICI group,P=0.004).The CMT+ICI group also demonstrated a significantly longer median progression-free survival of 11.7 months(P<0.001).Patients receiving combination therapy exhibited significantly improved systemic objective response rate and disease control rate.Multivariate analysis identified key factors significantly influencing OS in EC patients with liver metastases:Karnofsky Performance Status score≥70,receipt of local therapy for liver metastases,and the number of cycles of CMT and immunotherapy received.Furthermore,the incidence of TRAEs did not significantly differ between the CMT+ICI and CMT groups.CONCLUSION For EC patients with liver metastases,the combination of CMT and ICIs demonstrates significantly superior efficacy compared with CMT alone,while maintaining manageable TRAEs.展开更多
Dear Editor,Esophageal cancer(EC)is a malignant tumor originating from esophageal epithelium and remains a leading cause of cancer incidence and mortality worldwide1,2.According to the 2020 World Health Organization s...Dear Editor,Esophageal cancer(EC)is a malignant tumor originating from esophageal epithelium and remains a leading cause of cancer incidence and mortality worldwide1,2.According to the 2020 World Health Organization statistics,there were approx-imately 604,000 new EC cases and 544,000 EC-related deaths globally with China reporting approximately 320,000 new cases and 300,000 deaths,mainly from esophageal squamous cell carcinoma(ESCC)3.Although there has been progress in the treatment of EC,the long-term prognosis of patients with R0 resection and lymph node-positive disease contin-ues to be suboptimal4.A retrospective analysis performed by our center suggested that the median overall survival(OS)of lymph node-positive patients with EC who received postoper-ative adjuvant radiotherapy(PART)was 29 months compared to 21 months for surgery alone with 3-year survival rates of 43%and 36%,respectively,indicating a potential survival ben-efit of PART5.展开更多
Gastric cancer(GC)remains a major global health challenge,because of its poor prognosis and limited treatment options in advanced stages1,2.Recent advancements in immunotherapy,highlighted by the findings of the CHECK...Gastric cancer(GC)remains a major global health challenge,because of its poor prognosis and limited treatment options in advanced stages1,2.Recent advancements in immunotherapy,highlighted by the findings of the CHECKMATE-649,ORIENT-16,and KEYNOTE-859 trials,have markedly transformed the treatment paradigm for advanced gastric cancer(AGC)3-5.展开更多
Repairing the endothelial barrier is essential for maintaining pulmonary fuid balance and regulating leukocyte infiltration during sepsis[1].Tissue kallikrein-related peptidases(KLKs)are secreted serine proteases invo...Repairing the endothelial barrier is essential for maintaining pulmonary fuid balance and regulating leukocyte infiltration during sepsis[1].Tissue kallikrein-related peptidases(KLKs)are secreted serine proteases involved in angiogenesis[2].However,their involvement in regulating endothelial regeneration remains largely unknown.展开更多
Objective: Tumor-associated macrophages(TAMs) exhibit heterogeneous properties including anti-tumorigenic and protumorigenic phenotypes. The rate-limiting enzyme in de novo serine biosynthesis, 3-phosphoglycerate dehy...Objective: Tumor-associated macrophages(TAMs) exhibit heterogeneous properties including anti-tumorigenic and protumorigenic phenotypes. The rate-limiting enzyme in de novo serine biosynthesis, 3-phosphoglycerate dehydrogenase(PHGDH), has a well-established role in cellular metabolism, yet its specific role in macrophages remains unknown.Methods: Metabolomics assays were conducted to assess metabolite composition and dynamics in macrophages. Changes in polarization and immunosuppressive markers were validated with q RT-PCR. Bioinformatics was used to analyze immune cell subsets and associated metabolic pathways. Finally, Ch IP-q PCR and co-immunoprecipitation assays were performed to elucidate the downstream regulatory mechanisms of PHGDH.Results: Serine metabolism was found to be downregulated in TAMs in breast cancer. Functional studies revealed that PHGDH inhibition promotes an M2-like phenotype and immunosuppressive functions in macrophages. Furthermore, PHGDH was found to undergo nuclear translocation during macrophage polarization. Mechanistically, nuclear PHGDH was found to regulate GLUD1 and GLS2 transcription via interaction with the transcription factor STAT3. Rescue experiments demonstrated that glutamine supplementation and STAT3 inhibition reversed the effects of PHGDH on macrophage function.Conclusions: Our findings reveal a previously unrecognized non-canonical metabolic function of PHGDH, thus providing potential therapeutic targets in the tumor microenvironment for reversing malignant progression.展开更多
Stem cell-or tissue-derived three-dimensional organ-like formations,known as organoids,are emerging as effective tools in biomedicine.Since they may be useful in developing customized therapeutic solutions and efficie...Stem cell-or tissue-derived three-dimensional organ-like formations,known as organoids,are emerging as effective tools in biomedicine.Since they may be useful in developing customized therapeutic solutions and efficient drug screening protocols,organoids can deepen our understanding of novel disease mechanisms.In doing so,they can facilitate advancements in drug discovery platforms,pharmacological safety,and clinical trials.This review explores various biomedical applications of organoids,including drug development and disease modeling,and highlights specific tools and analytical techniques that can be employed to investigate organoids and their microenvironments.Finally,we review recent clinical trials and patents related to organoids that show great promise for future clinical translation.展开更多
Objective:The key molecular events signifying the Helicobacter pylori-induced gastric carcinogenesis process are largely unknown.Methods:Bulk tissue-proteomics profiling were leveraged across multi-stage gastric lesio...Objective:The key molecular events signifying the Helicobacter pylori-induced gastric carcinogenesis process are largely unknown.Methods:Bulk tissue-proteomics profiling were leveraged across multi-stage gastric lesions from Linqu(n=166)and Beijing sets(n=99)and single-cell transcriptomic profiling(n=18)to decipher key molecular signatures of H.pylori-related gastric lesion progression and gastric cancer(GC)development.The association of key proteins association with gastric lesion progression and GC development were prospectively studied building on follow-up of the Linqu set and UK Biobank(n=48,529).Results:Concordant proteomics signatures associated with H.pylori infection and gastric carcinogenesis(ρ=0.784,correlation P=1.80×10^(−36))were identified.RNA expression of genes encoding 13 up-and 15 down-regulated key proteins displayed trending alterations in the transition from normal gastric epithelium to intestinal metaplasia,then to malignant cells.A 15-tissue protein panel integrating these signatures demonstrated potential for targeting individuals at high risk for progressing to gastric neoplasia(OR=7.22,95%CI:1.31-39.72 for the high-score group).A 4-circulating protein panel may be used as non-invasive markers predicting the risk of GC development(hazard ratio=3.73,95%confidence interval:1.63-8.54,high-risk vs.low-risk populations,area under the curve=0.75).Conclusions:Concordant proteomics signatures associated with H.pylori infection and gastric carcinogenesis were unveiled with potential as biomarkers for targeted prevention strategies.展开更多
Malignant tumors are a major threat to human health with the immune responses critically influenced by major histocompatibility complex(MHC)class I and Ⅱ molecules.While MHC-I has been extensively studied for its rol...Malignant tumors are a major threat to human health with the immune responses critically influenced by major histocompatibility complex(MHC)class I and Ⅱ molecules.While MHC-I has been extensively studied for its role in tumor immunity,research on MHC-Ⅱ,particularly MHC-Ⅱ function within the tumor microenvironment,has lagged behind research on MHC-Ⅰ.The expression and regulation of tumor-specific MHC-Ⅱ(tsMHC-Ⅱ)in tumor cells not only reflect the immunogenic landscape of the tumor microenvironment but also actively shape antitumor immune responses by modulating CD4^(+)T cell recognition and activation.Expression of tsMHC-Ⅱ is tightly controlled by intrinsic oncogenic signaling and extrinsic cytokine stimulation,positioning tsMHC-Ⅱ as a key determinant of response to immunotherapy,including immune checkpoint blockade.Accordingly,tsMHC-Ⅱ may serve as a predictive biomarker and a potential therapeutic target in tumor immunotherapy.This review highlights recent advances in the structure and function of MHC-Ⅱ,the MHC-Ⅱ regulatory mechanisms in tumors,and the emerging significance of MHC-Ⅱ in guiding future immunotherapeutic strategies.展开更多
Recently,stimuli-responsive nanocarriers capable of precision drug release have garnered significant attention in the field of drug delivery.Here,an in-situ dynamic covalent self-assembled(DCS)strategy was utilized to...Recently,stimuli-responsive nanocarriers capable of precision drug release have garnered significant attention in the field of drug delivery.Here,an in-situ dynamic covalent self-assembled(DCS)strategy was utilized to develop a co-delivery system.This assembly was based on a thiol-disulfide-exchange reaction,producing disulfide macrocycles in an oxidizing aerial environment.These macrocycles encapsulated the anti-cancer drug(paclitaxel,PTX)on the surface of gold nanoparticles,which served as photothermal therapy agents during the self-assembly.In the DCS process,the kinetic control over the concentration of each building unit within the reaction system led to the formation of a stable co-delivery nanosystem with optimal drug-loading efficiency.Notably,the high glutathione(GSH)concentrations in tumor cells caused the disulfide macrocycles in nanostructures to break,resulting in drug release.The stimuli-responsive performances of the prepared nanosystems were determined by observing the molecular structures and drug release.The results revealed that the self-assembled nanosystem exhibited GSH-triggered drug release and good photothermal conversion capability under near-infrared light.Moreover,the in vitro and in vivo results revealed that conjugating the targeting molecule of cRGD with co-delivery nanosystem enhanced its biocompatibility,chemo-photothermal anti-cancer effect.Overall,our findings indicated that in-situ DCS strategy enhanced the control over drug loading during the construction of the co-delivery system,paving a way for the development of more functional carriers in nanomedicine.展开更多
Molecular medicine,which delves into the intricacies of biomolecular structure,function,and role,is pivotal for advancing precise diagnostics and personalized treatment.Nucleic acids,a class of star functional molecul...Molecular medicine,which delves into the intricacies of biomolecular structure,function,and role,is pivotal for advancing precise diagnostics and personalized treatment.Nucleic acids,a class of star functional molecules,are notable for their versatile applications in molecular diagnostics,gene therapy,and drug development.Therefore,in this study,we review the extensive use of nucleic acid aptamers in medicinal practice.Furthermore,the expanding field of molecular medicine has catalyzed advancements in traditional Chinese medicine(TCM),as evidenced by scientific endeavors to integrate modern technologies.Therefore,TCM has experienced rapid modernization by leveraging artificial intelligence,nucleic acid molecular medicine,and bioelectronic medicine.展开更多
Diabetes and insulinoma represent opposing alterations in pancreatic β-cell mass,with diabetes resulting from irreversible β-cells damage and insulinoma arising from abnormal proliferation.Early diagnosis of both co...Diabetes and insulinoma represent opposing alterations in pancreatic β-cell mass,with diabetes resulting from irreversible β-cells damage and insulinoma arising from abnormal proliferation.Early diagnosis of both conditions necessitates effectiveβ-cell mass detection.Current detection methods are limited in diagnosing each condition individually or lacking timely and accurate detection.Diabetes is typically identified only after significantβ-cell loss,while insulinoma can evade conventional imaging due to their small size.Positron emission tomography/computed tomography(PET/CT)imaging,combining anatomical and functional data,enhances diagnostic accuracy but faces challenges in specificity.This study employed two RNA aptamers(m12–3773 and 1–717)modified to enhance RNase resistance and conjugated with68Ga to create ^(68)Ga-NOTA-Ap.^(68)Ga-NOTA-Ap was administered to rats with pancreaticβ-cell damage and mice with insulinoma to evaluate its ability to image islets,detect changes in pancreatic β-cell mass(BCM),and identify insulinoma.Modified with methoxy and fluoro,RNA aptamers exhibited enhanced stability and RNases resistance while retaining their dissociation constants(K_(d)).Furthermore,^(68)Ga-NOTA-Ap effectively detected changes of BCM in rats with pancreatic β-cell damage and imaged insulinoma in mice through recognition of abnormalβ-cell proliferation by recognizing clusterin and transmembrane p24 trafficking protein 6(TMED6)on pancreaticβ-cell.The developed ^(68)Ga-NOTA-Ap shows promise for early screening of diabetes and insulinoma due to its high sensitivity,specificity,and non-invasive nature.It has potential clinical applications for monitoring pancreatic β-cell function and diagnosing insulinoma.展开更多
Objective:Plasma cell-free DNA(cfDNA)methylation has shown potential in the detection and prognostic testing of multiple cancers.Here,we comprehensively investigate the performance of cfDNA methylation for gastric can...Objective:Plasma cell-free DNA(cfDNA)methylation has shown potential in the detection and prognostic testing of multiple cancers.Here,we comprehensively investigate the performance of cfDNA methylation for gastric cancer(GC)detection and prognosis.Methods:GC-specific differentially methylated regions(DMRs)were identified by sequencing 56 GC tissues and 59 normal adjacent tissues(NATs).We then performed targeted bisulfite sequencing of cfDNA from 294 GC and 446 non-gastric cancer(NGC)plasma samples,identifying 179 DMRs that overlapped with those in tissue samples.The efficacy of plasma cfDNA methylation markers for GC detection and prognosis was evaluated.Results:Based on the 179 DMRs overlapping with those in tissue samples,the random forest(RF)model using28 DMRs achieved an area under the curve(AUC)of 0.998 in the training cohort,whereas further refinement to the top 6 DMRs resulted in an AUC of 0.985.Consistent results were obtained in the validation cohort(28 DMR AUC:0.985;6 DMR AUC:0.988).Support vector machine(SVM)and logistic regression(LR)models also demonstrated robust performance.Additionally,an 11-DMR signature was developed for prognostic prediction,successfully identifying high-risk GC patients with significantly shorter overall survival.Conclusions:Our study highlights the potential utility of cfDNA methylation markers for both the detection and prognostication of GC.展开更多
BACKGROUND The log odds of positive lymph nodes(LODDS)are correlated with survival outcomes in gastric cancer(GC)patients.However,the prognostic value across different tumor differentiation levels remains unclear.AIM ...BACKGROUND The log odds of positive lymph nodes(LODDS)are correlated with survival outcomes in gastric cancer(GC)patients.However,the prognostic value across different tumor differentiation levels remains unclear.AIM To evaluate the independent prognostic value of LODDS and the stratified predictive efficacy in GC patients with different histologic differentiations.METHODS We conducted a retrospective analysis of 2103 GC patients who underwent radical gastrectomy at Zhejiang Cancer Hospital.The prognostic value of LODDS was compared with that of other lymph node-based metrics,including the pathologic N stage,number of positive lymph nodes,number of total lymph nodes,and lymph node ratio,stratified by tumor differentiation.RESULTS LODDS was identified as an independent prognostic factor for overall survival in moderately to poorly differentiated GC patients.LODDS demonstrated superior predictive accuracy over other lymph node metrics.A nomogram incorporating LODDS,age,carbohydrate antigen(CA)125,carcinoembryonic antigen,and tumor differentiation showed good predictive accuracy(C-index=0.703).A higher LODDS was significantly associated with an increased risk of recurrence or metastasis,poorly differentiated tumors,advanced cancer,mucinous gastric adenocarcinoma,nerve invasion,and vascular tumor thrombus.Additionally,LODDS was positively correlated with the tumor markers CA19-9,CA72-4,CA125,and CA242(all P<0.05).CONCLUSION LODDS is an independent prognostic indicator for patients with moderately and poorly differentiated GC,and its predictive performance is superior to that of other models.展开更多
Hemoptysis is defined as bleeding originating from the respiratory tract distal to the larynx and is associated with a wide spectrum of underlying conditions,including bronchiectasis,pulmonary malignancies,tuberculosi...Hemoptysis is defined as bleeding originating from the respiratory tract distal to the larynx and is associated with a wide spectrum of underlying conditions,including bronchiectasis,pulmonary malignancies,tuberculosis,aspergillosis,and vascular malformations.^([1-3]) A metaanalysis involving patients with massive hemoptysis reported a mortality rate of 3.5%.^([4])This underscores the critical importance of prompt and eff ective embolization of the responsible artery to improve outcomes,particularly in patients presenting with life-threatening hemoptysis.展开更多
BACKGROUND Esophageal cancer patients had the highest intensive care unit(ICU)admitted rate in cancer patients.But their prognosis and evaluation methods were rarely studied.AIM To depict the short-term mortality outc...BACKGROUND Esophageal cancer patients had the highest intensive care unit(ICU)admitted rate in cancer patients.But their prognosis and evaluation methods were rarely studied.AIM To depict the short-term mortality outcome and identify the potential prognostic factors of esophageal cancer patients admitted into ICU.METHODS A multicenter cross-sectional study was performed from May 10,2021 to July 10,2021 at ICU departments of 37 cancer specialized hospitals in China.Patients aged≥14 years with ICU duration≥24 hours were included.Clinical records of patients with primary esophageal cancer diagnosis were reviewed.Patients were separated into groups according to the 90 days survival.Characteristics between groups were compared.Single and multi-variate regression tests were applied to analyze the correlated factors of ICU outcomes.Predictive values of disease severity scores were assessed using receiver operating characteristic curve analysis.RESULTS Total 180 esophageal cancer patients were included.The 90 days mortality was 22.2%.Patients with mortality outcome showed differences from those survived mostly in disease severity and unplanned transfer from clinical ward.The current evaluation tools,including Sequential Organ Failure Assessment and Acute Physiology and Chronic Health Evaluation II scores had low accuracy in prediction of short-term death.ICU admitted esophageal cancer patients have poor prognosis,especially those with acute illness.CONCLUSION The prognostic tools for these patients need to be further optimized.展开更多
BACKGROUND Colorectal cancer(CRC)is one of the most common cancers and CRC patients are among the most common intensive care unit(ICU)admitted cancer patients.However,their prognosis and evaluation methods are rarely ...BACKGROUND Colorectal cancer(CRC)is one of the most common cancers and CRC patients are among the most common intensive care unit(ICU)admitted cancer patients.However,their prognosis and evaluation methods are rarely studied.AIM To determine the short-term mortality outcome and identify the potential prognostic factors of CRC cancer patients admitted to the ICU.METHODS A multicenter cross-sectional study was performed from May 10,2021 to July 10,2021 at the ICU departments of 37 cancer specialized hospitals in China,and included patients aged≥14 years with ICU duration≥24 hours.Clinical records of patients with a primary CRC diagnosis were reviewed.Patients were separated into groups according to 90-day survival.Characteristics between groups were compared.Univariate and multivariate regression tests were used to analyze the correlated factors of ICU outcomes.Predictive values of disease severity scores were assessed using receiver operating characteristic curve analysis.RESULTS In total,189 CRC patients were included in the study.The 90-day mortality was 12.2%.Patients who died showed differences compared to patients who survived mostly in terms of disease severity and ICU complications.It appears that patients admitted to the ICU from a clinical ward due to emergencies may have a higher risk of mortality while surgical management was associated with better survival.In multivariate analysis,only chemotherapy,elective surgery and conventional oxygen therapy were identified as independently correlated with 90-day mortality.Sequential organ failure assessment and acute physiology and chronic health evaluation II scores had moderate accuracy in predicting short-term mortality.CONCLUSION ICU admitted CRC patients appear to have low short-term mortality which requires further confirmation in prospective studies.The prognostic tools for these patients need further optimization.展开更多
Breast cancer is a malignant tumor that seriously endangers women's lives. The prognosis of breast cancer patients differs among molecular types. Compared with other subtypes, triple-negative breast cancer(TNBC) h...Breast cancer is a malignant tumor that seriously endangers women's lives. The prognosis of breast cancer patients differs among molecular types. Compared with other subtypes, triple-negative breast cancer(TNBC) has been a research hotspot in recent years because of its high degree of malignancy, strong invasiveness, rapid progression, easy of recurrence,distant metastasis, poor prognosis, and high mortality. Many studies have found that long non-coding RNA(lncRNA) plays an important role in the occurrence, proliferation, migration, recurrence, chemotherapy resistance, and other characteristics of TNBC. Some lncRNAs are expected to become biomarkers in the diagnosis and prognosis of TNBC, and even new targets for its treatment. Based on a PubMed literature search, this review summarizes the progress in research on lncRNAs in TNBC and discusses their roles in TNBC diagnosis, prognosis, and chemotherapy with the hope of providing help for future research.展开更多
基金supported by the Zhejiang Province Traditional Chinese Medicine Science and Technology Project(GZY-ZJ-KJ-24063)the Natural Science Foundation of Zhejiang Province(Q24H290031)the Key Laboratory for Molecular Medicine and Chinese Medicine Preparations(No.GZY-ZJ-SY-2303).
文摘Immunotherapies have demonstrated notable clinical benefits in the treatment of cervical cancer(CC).However,the development of therapeutic resistance and diverse adverse effects in immunotherapy stem from complex interactions among biological processes and factors within the tumor immune microenvironment(TIME).Advanced omic technologies offer novel insights into a more expansive and thorough layer of the TIME.Furthermore,integrating multidimensional omics within the frameworks of systems biology and computational methodologies facilitates the generation of interpretable data outputs to characterize the clinical and biological trajectories of tumor behavior.In this review,we present advanced omics technologies that utilize various clinical samples to address scientific inquiries related to immunotherapies for CC,highlighting their utility in identifying metastasis dissemination,recurrence risk,and therapeutic resistance in patients treated with immunotherapeutic approaches.This review elaborates on the strategy for integrating multi-omics data through artificial intelligence algorithms.Additionally,an analysis of the obstacles encountered in the multi-omics analysis process and potential avenues for future research in this domain are presented.
文摘Non-right-handedness(NRH),encompassing left-handedness and mixed-handedness,has been frequently reported at elevated rates in individuals with various psychiatric disorders.The consistency of this association across multiple conditions and its underlying mechanisms is the subject of ongoing investigation.This review synthesized current evidence to explore the association between NRH and psychiatric disorders from epidemiological,genetic,and neurobiological perspectives.We systematically identified and appraised relevant literature investigating NRH prevalence in psychiatric populations and potential explanatory mechanisms.Epidemiological evidence indicates an elevated prevalence of NRH,particularly within neurodevelopmental disorders.Potential contributing mechanisms identified include early developmental disruptions,shared genetic predispositions,and atypical patterns of brain lateralization.While the association between NRH and psychiatric conditions,especially neurodevelopmental disorders,is evident,the causal pathways and relative contributions of identified mechanisms are complex and debated.This review highlighted key areas requiring further research to elucidate these relationships.
基金Supported by National Natural Science Foundation of China,No.82303672Zhejiang Provincial Health Commission and Zhejiang Provincial Administration of Traditional Chinese Medicine through the Targeted Project for Medical and Health Research,No.2025ZL017and China Primary Health Care Foundation,No.ZLMY20240311001ZJ.
文摘BACKGROUND The liver represents a common site of distant metastasis in patients with esophageal cancer(EC).Conventional chemotherapy(CMT)presents limited efficacy for EC,and EC patients with liver metastases typically experience a poor prognosis,highlighting an urgent need to explore novel treatment approaches.This study evaluated the overall efficacy and safety of CMT vs CMT combined with immune checkpoint inhibitors(ICIs)in the treatment of EC patients with liver metastases.Furthermore,prognostic factors influencing outcomes in this patient population were identified.AIM To evaluate the efficacy and safety of first-line chemoimmunotherapy for EC patients with liver metastases and to analyze prognostic factors.METHODS This retrospective study included 126 EC patients with liver metastases at Zhejiang Cancer Hospital between 2014 and 2024.Patients receiving CMT were compared with those receiving CMT+ICI.Analyzed variables included clinicopathological features,treatment history,characteristics of metastasis,systemic and local treatments,overall survival(OS),and treatment-related adverse events(TRAEs).Prognostic factors were evaluated using univariate and multivariate Cox proportional-hazards regression models.Finally,efficacy outcomes and TRAE profiles were compared between the two groups.RESULTS A significant difference in median OS was identified between the two groups(10.8 months in the CMT group vs 20.8 months in the CMT+ICI group,P=0.004).The CMT+ICI group also demonstrated a significantly longer median progression-free survival of 11.7 months(P<0.001).Patients receiving combination therapy exhibited significantly improved systemic objective response rate and disease control rate.Multivariate analysis identified key factors significantly influencing OS in EC patients with liver metastases:Karnofsky Performance Status score≥70,receipt of local therapy for liver metastases,and the number of cycles of CMT and immunotherapy received.Furthermore,the incidence of TRAEs did not significantly differ between the CMT+ICI and CMT groups.CONCLUSION For EC patients with liver metastases,the combination of CMT and ICIs demonstrates significantly superior efficacy compared with CMT alone,while maintaining manageable TRAEs.
基金supported by the National Natural Science Foundation of China(Grant No.82172567)the Key R&D Plan of Jiangxi Province(Grant No.2021BBG71006)the Key Project of Science and Technology Innovation of Health Commission of Jiangxi Province(Grant Nos.2023ZD005 and 2024ZD008).
文摘Dear Editor,Esophageal cancer(EC)is a malignant tumor originating from esophageal epithelium and remains a leading cause of cancer incidence and mortality worldwide1,2.According to the 2020 World Health Organization statistics,there were approx-imately 604,000 new EC cases and 544,000 EC-related deaths globally with China reporting approximately 320,000 new cases and 300,000 deaths,mainly from esophageal squamous cell carcinoma(ESCC)3.Although there has been progress in the treatment of EC,the long-term prognosis of patients with R0 resection and lymph node-positive disease contin-ues to be suboptimal4.A retrospective analysis performed by our center suggested that the median overall survival(OS)of lymph node-positive patients with EC who received postoper-ative adjuvant radiotherapy(PART)was 29 months compared to 21 months for surgery alone with 3-year survival rates of 43%and 36%,respectively,indicating a potential survival ben-efit of PART5.
基金supported by The National Key Research and Development Program of China(Grant no.2021YFA0910100)Healthy Zhejiang One Million People Cohort(Grant no.K-20230085)+5 种基金Post-doctoral Innovative Talent Support Program(Grant no.BX2023375)Lingyan Project of Zhejiang Provincial Department of Science and Technology(Grant no.2025C02059)the National Natural Science Foundation of China(Grant nos.82304946,82473489,and 82403546)Natural Science Foundation of Zhejiang Province(Grant nos.LR21H280001,LGF22H160056,ZCLQN25H1602,and LMS25H160006)Medicine and Health Science Fund of Zhejiang Province Health Commission(Grant nos.2025KY047 and 2022KY658)Traditional Chinese Medicine Science and Technology Project of Zhejiang Provincial Health Commission(Grant no.2022ZA023).
文摘Gastric cancer(GC)remains a major global health challenge,because of its poor prognosis and limited treatment options in advanced stages1,2.Recent advancements in immunotherapy,highlighted by the findings of the CHECKMATE-649,ORIENT-16,and KEYNOTE-859 trials,have markedly transformed the treatment paradigm for advanced gastric cancer(AGC)3-5.
基金supported by the National Natural Science Foundation of China(Grant Nos.:32171124,31871156,31971101,32271180,82272229,and 81471852)Hunan Provincial Natural Science Foundation of China(Grant No.:2021JJ31058).
文摘Repairing the endothelial barrier is essential for maintaining pulmonary fuid balance and regulating leukocyte infiltration during sepsis[1].Tissue kallikrein-related peptidases(KLKs)are secreted serine proteases involved in angiogenesis[2].However,their involvement in regulating endothelial regeneration remains largely unknown.
基金supported by grants from the National Key R&D Program of China (Grant No. 2022YFC3401001)National Natural Science Foundation of China (Grant Nos. 82025026 and 82230091 to H.H.)+1 种基金Key R&D Program of Zhejiang (Grant No. 2024C03160)Guang Dong Basic and Applied Basic Research Foundation (Grant Nos. 2023A1515012412 and 2023A1515011214)。
文摘Objective: Tumor-associated macrophages(TAMs) exhibit heterogeneous properties including anti-tumorigenic and protumorigenic phenotypes. The rate-limiting enzyme in de novo serine biosynthesis, 3-phosphoglycerate dehydrogenase(PHGDH), has a well-established role in cellular metabolism, yet its specific role in macrophages remains unknown.Methods: Metabolomics assays were conducted to assess metabolite composition and dynamics in macrophages. Changes in polarization and immunosuppressive markers were validated with q RT-PCR. Bioinformatics was used to analyze immune cell subsets and associated metabolic pathways. Finally, Ch IP-q PCR and co-immunoprecipitation assays were performed to elucidate the downstream regulatory mechanisms of PHGDH.Results: Serine metabolism was found to be downregulated in TAMs in breast cancer. Functional studies revealed that PHGDH inhibition promotes an M2-like phenotype and immunosuppressive functions in macrophages. Furthermore, PHGDH was found to undergo nuclear translocation during macrophage polarization. Mechanistically, nuclear PHGDH was found to regulate GLUD1 and GLS2 transcription via interaction with the transcription factor STAT3. Rescue experiments demonstrated that glutamine supplementation and STAT3 inhibition reversed the effects of PHGDH on macrophage function.Conclusions: Our findings reveal a previously unrecognized non-canonical metabolic function of PHGDH, thus providing potential therapeutic targets in the tumor microenvironment for reversing malignant progression.
基金supported by the National Natural Science Foundation of China(No.82172567)Key R&D Plan of Jiangxi Province(No.2021BBG71006)the Key Project of Science and Technology Innovation of Health Commission of Jiangxi Province(Nos.2023ZD005 and 2024ZD008)。
文摘Stem cell-or tissue-derived three-dimensional organ-like formations,known as organoids,are emerging as effective tools in biomedicine.Since they may be useful in developing customized therapeutic solutions and efficient drug screening protocols,organoids can deepen our understanding of novel disease mechanisms.In doing so,they can facilitate advancements in drug discovery platforms,pharmacological safety,and clinical trials.This review explores various biomedical applications of organoids,including drug development and disease modeling,and highlights specific tools and analytical techniques that can be employed to investigate organoids and their microenvironments.Finally,we review recent clinical trials and patents related to organoids that show great promise for future clinical translation.
基金funded by the National Natural Science Foundation of China(Grant No.82273704)Noncommunicable Chronic Diseases-National Science and Technology Major Project(Grant Nos.2023ZD0501400-2023ZD0501402)+3 种基金Beijing Hospitals Authority’s Ascent Plan(Grant No.DFL20241102)Beijing Hospitals Authority Clinical Medicine Development of Special Funding Support(Grant No.ZLRK202325)Peking University Medicine Fund for World’s Leading Discipline or Discipline Cluster Development(Grant No.BMU2022XKQ004)the Science Foundation of Peking University Cancer Hospital(Grant Nos.BJCH2024BJ02,XKFZ2410,and 2022-27).
文摘Objective:The key molecular events signifying the Helicobacter pylori-induced gastric carcinogenesis process are largely unknown.Methods:Bulk tissue-proteomics profiling were leveraged across multi-stage gastric lesions from Linqu(n=166)and Beijing sets(n=99)and single-cell transcriptomic profiling(n=18)to decipher key molecular signatures of H.pylori-related gastric lesion progression and gastric cancer(GC)development.The association of key proteins association with gastric lesion progression and GC development were prospectively studied building on follow-up of the Linqu set and UK Biobank(n=48,529).Results:Concordant proteomics signatures associated with H.pylori infection and gastric carcinogenesis(ρ=0.784,correlation P=1.80×10^(−36))were identified.RNA expression of genes encoding 13 up-and 15 down-regulated key proteins displayed trending alterations in the transition from normal gastric epithelium to intestinal metaplasia,then to malignant cells.A 15-tissue protein panel integrating these signatures demonstrated potential for targeting individuals at high risk for progressing to gastric neoplasia(OR=7.22,95%CI:1.31-39.72 for the high-score group).A 4-circulating protein panel may be used as non-invasive markers predicting the risk of GC development(hazard ratio=3.73,95%confidence interval:1.63-8.54,high-risk vs.low-risk populations,area under the curve=0.75).Conclusions:Concordant proteomics signatures associated with H.pylori infection and gastric carcinogenesis were unveiled with potential as biomarkers for targeted prevention strategies.
基金supported by The National Key Research and Development Program of China(2021YFA0910100)Healthy Zhejiang One Million People Cohort(K-20230085)+4 种基金National Natural Science Foundation of China(82304946,82473489,and 82403546)Post-doctoral Innovative Talent Support Program(BX2023375)Natural Science Foundation of Zhejiang Province(LR21H280001)China Postdoctoral Science Foundation(2023M743560)The Medicine and Health Science Fund of Zhejiang Province Health Commission(2025KY047).
文摘Malignant tumors are a major threat to human health with the immune responses critically influenced by major histocompatibility complex(MHC)class I and Ⅱ molecules.While MHC-I has been extensively studied for its role in tumor immunity,research on MHC-Ⅱ,particularly MHC-Ⅱ function within the tumor microenvironment,has lagged behind research on MHC-Ⅰ.The expression and regulation of tumor-specific MHC-Ⅱ(tsMHC-Ⅱ)in tumor cells not only reflect the immunogenic landscape of the tumor microenvironment but also actively shape antitumor immune responses by modulating CD4^(+)T cell recognition and activation.Expression of tsMHC-Ⅱ is tightly controlled by intrinsic oncogenic signaling and extrinsic cytokine stimulation,positioning tsMHC-Ⅱ as a key determinant of response to immunotherapy,including immune checkpoint blockade.Accordingly,tsMHC-Ⅱ may serve as a predictive biomarker and a potential therapeutic target in tumor immunotherapy.This review highlights recent advances in the structure and function of MHC-Ⅱ,the MHC-Ⅱ regulatory mechanisms in tumors,and the emerging significance of MHC-Ⅱ in guiding future immunotherapeutic strategies.
基金supported by the National Natural Science Foundation of China(Nos.82202274,82072032,22161016,32025021,12374390,52002380 and 31971292),the National Science and Technology Major Project(No.2023ZD0500902)the Fellowship of China Postdoctoral Science Foundation(No.2023M743559)+2 种基金the Member of Youth Innovation Promotion Association Foundation of CAS,China(No.2023310)the Key Scientific and Technological Special Project of Ningbo City(No.2023Z209)the Natural Science Foundation of Zhejiang Province(No.LQ23H180003)。
文摘Recently,stimuli-responsive nanocarriers capable of precision drug release have garnered significant attention in the field of drug delivery.Here,an in-situ dynamic covalent self-assembled(DCS)strategy was utilized to develop a co-delivery system.This assembly was based on a thiol-disulfide-exchange reaction,producing disulfide macrocycles in an oxidizing aerial environment.These macrocycles encapsulated the anti-cancer drug(paclitaxel,PTX)on the surface of gold nanoparticles,which served as photothermal therapy agents during the self-assembly.In the DCS process,the kinetic control over the concentration of each building unit within the reaction system led to the formation of a stable co-delivery nanosystem with optimal drug-loading efficiency.Notably,the high glutathione(GSH)concentrations in tumor cells caused the disulfide macrocycles in nanostructures to break,resulting in drug release.The stimuli-responsive performances of the prepared nanosystems were determined by observing the molecular structures and drug release.The results revealed that the self-assembled nanosystem exhibited GSH-triggered drug release and good photothermal conversion capability under near-infrared light.Moreover,the in vitro and in vivo results revealed that conjugating the targeting molecule of cRGD with co-delivery nanosystem enhanced its biocompatibility,chemo-photothermal anti-cancer effect.Overall,our findings indicated that in-situ DCS strategy enhanced the control over drug loading during the construction of the co-delivery system,paving a way for the development of more functional carriers in nanomedicine.
基金supported by the National Natural Science Foundation of China(T2188102)Hangzhou Institute of Medicine(2024ZZBS02,Hangzhou,China).
文摘Molecular medicine,which delves into the intricacies of biomolecular structure,function,and role,is pivotal for advancing precise diagnostics and personalized treatment.Nucleic acids,a class of star functional molecules,are notable for their versatile applications in molecular diagnostics,gene therapy,and drug development.Therefore,in this study,we review the extensive use of nucleic acid aptamers in medicinal practice.Furthermore,the expanding field of molecular medicine has catalyzed advancements in traditional Chinese medicine(TCM),as evidenced by scientific endeavors to integrate modern technologies.Therefore,TCM has experienced rapid modernization by leveraging artificial intelligence,nucleic acid molecular medicine,and bioelectronic medicine.
基金supported by National Natural Science Foundation of China(No.82002241)National Key Research and Development Program of China(No.2020YFA0909000)“Clinic Plus”Outstanding Project(No.2024ZY012)from Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine。
文摘Diabetes and insulinoma represent opposing alterations in pancreatic β-cell mass,with diabetes resulting from irreversible β-cells damage and insulinoma arising from abnormal proliferation.Early diagnosis of both conditions necessitates effectiveβ-cell mass detection.Current detection methods are limited in diagnosing each condition individually or lacking timely and accurate detection.Diabetes is typically identified only after significantβ-cell loss,while insulinoma can evade conventional imaging due to their small size.Positron emission tomography/computed tomography(PET/CT)imaging,combining anatomical and functional data,enhances diagnostic accuracy but faces challenges in specificity.This study employed two RNA aptamers(m12–3773 and 1–717)modified to enhance RNase resistance and conjugated with68Ga to create ^(68)Ga-NOTA-Ap.^(68)Ga-NOTA-Ap was administered to rats with pancreaticβ-cell damage and mice with insulinoma to evaluate its ability to image islets,detect changes in pancreatic β-cell mass(BCM),and identify insulinoma.Modified with methoxy and fluoro,RNA aptamers exhibited enhanced stability and RNases resistance while retaining their dissociation constants(K_(d)).Furthermore,^(68)Ga-NOTA-Ap effectively detected changes of BCM in rats with pancreatic β-cell damage and imaged insulinoma in mice through recognition of abnormalβ-cell proliferation by recognizing clusterin and transmembrane p24 trafficking protein 6(TMED6)on pancreaticβ-cell.The developed ^(68)Ga-NOTA-Ap shows promise for early screening of diabetes and insulinoma due to its high sensitivity,specificity,and non-invasive nature.It has potential clinical applications for monitoring pancreatic β-cell function and diagnosing insulinoma.
基金supported by National Key R&D Program of China(No.2021YFA0910100)National Natural Science Foundation of China(No.82374544,82204828,82422078)+2 种基金Healthy Zhejiang One Million People Cohort(No.K-20230085)Program of Zhejiang Provincial TCM Sci-tech Plan(No.GZY-ZJ-KJ-230003,No.GZY-ZJ-KJ23048)Natural Science Foundation of Zhejiang Province(No.LHDMY22H160008)。
文摘Objective:Plasma cell-free DNA(cfDNA)methylation has shown potential in the detection and prognostic testing of multiple cancers.Here,we comprehensively investigate the performance of cfDNA methylation for gastric cancer(GC)detection and prognosis.Methods:GC-specific differentially methylated regions(DMRs)were identified by sequencing 56 GC tissues and 59 normal adjacent tissues(NATs).We then performed targeted bisulfite sequencing of cfDNA from 294 GC and 446 non-gastric cancer(NGC)plasma samples,identifying 179 DMRs that overlapped with those in tissue samples.The efficacy of plasma cfDNA methylation markers for GC detection and prognosis was evaluated.Results:Based on the 179 DMRs overlapping with those in tissue samples,the random forest(RF)model using28 DMRs achieved an area under the curve(AUC)of 0.998 in the training cohort,whereas further refinement to the top 6 DMRs resulted in an AUC of 0.985.Consistent results were obtained in the validation cohort(28 DMR AUC:0.985;6 DMR AUC:0.988).Support vector machine(SVM)and logistic regression(LR)models also demonstrated robust performance.Additionally,an 11-DMR signature was developed for prognostic prediction,successfully identifying high-risk GC patients with significantly shorter overall survival.Conclusions:Our study highlights the potential utility of cfDNA methylation markers for both the detection and prognostication of GC.
基金Supported by the National Natural Science Foundation of China,No.82473195 and No.32370797the Natural Science Foundation of Zhejiang Province,No.LTGY23H160018+3 种基金the Zhejiang Medical and Health Science and Technology Program,No.2024KY789 and No.2023KY615the National Research Center for Translational Medicine at Shanghai Program,No.NRCTM(SH)-2025-07the Beijing Science and Technology Innovation Medical Development Foundation,No.KC2023-JX-0270-07the Key Laboratory of Prevention,Diagnosis and Therapy of Upper Gastrointestinal Cancer of Zhejiang Province,No.2022E10021.
文摘BACKGROUND The log odds of positive lymph nodes(LODDS)are correlated with survival outcomes in gastric cancer(GC)patients.However,the prognostic value across different tumor differentiation levels remains unclear.AIM To evaluate the independent prognostic value of LODDS and the stratified predictive efficacy in GC patients with different histologic differentiations.METHODS We conducted a retrospective analysis of 2103 GC patients who underwent radical gastrectomy at Zhejiang Cancer Hospital.The prognostic value of LODDS was compared with that of other lymph node-based metrics,including the pathologic N stage,number of positive lymph nodes,number of total lymph nodes,and lymph node ratio,stratified by tumor differentiation.RESULTS LODDS was identified as an independent prognostic factor for overall survival in moderately to poorly differentiated GC patients.LODDS demonstrated superior predictive accuracy over other lymph node metrics.A nomogram incorporating LODDS,age,carbohydrate antigen(CA)125,carcinoembryonic antigen,and tumor differentiation showed good predictive accuracy(C-index=0.703).A higher LODDS was significantly associated with an increased risk of recurrence or metastasis,poorly differentiated tumors,advanced cancer,mucinous gastric adenocarcinoma,nerve invasion,and vascular tumor thrombus.Additionally,LODDS was positively correlated with the tumor markers CA19-9,CA72-4,CA125,and CA242(all P<0.05).CONCLUSION LODDS is an independent prognostic indicator for patients with moderately and poorly differentiated GC,and its predictive performance is superior to that of other models.
文摘Hemoptysis is defined as bleeding originating from the respiratory tract distal to the larynx and is associated with a wide spectrum of underlying conditions,including bronchiectasis,pulmonary malignancies,tuberculosis,aspergillosis,and vascular malformations.^([1-3]) A metaanalysis involving patients with massive hemoptysis reported a mortality rate of 3.5%.^([4])This underscores the critical importance of prompt and eff ective embolization of the responsible artery to improve outcomes,particularly in patients presenting with life-threatening hemoptysis.
文摘BACKGROUND Esophageal cancer patients had the highest intensive care unit(ICU)admitted rate in cancer patients.But their prognosis and evaluation methods were rarely studied.AIM To depict the short-term mortality outcome and identify the potential prognostic factors of esophageal cancer patients admitted into ICU.METHODS A multicenter cross-sectional study was performed from May 10,2021 to July 10,2021 at ICU departments of 37 cancer specialized hospitals in China.Patients aged≥14 years with ICU duration≥24 hours were included.Clinical records of patients with primary esophageal cancer diagnosis were reviewed.Patients were separated into groups according to the 90 days survival.Characteristics between groups were compared.Single and multi-variate regression tests were applied to analyze the correlated factors of ICU outcomes.Predictive values of disease severity scores were assessed using receiver operating characteristic curve analysis.RESULTS Total 180 esophageal cancer patients were included.The 90 days mortality was 22.2%.Patients with mortality outcome showed differences from those survived mostly in disease severity and unplanned transfer from clinical ward.The current evaluation tools,including Sequential Organ Failure Assessment and Acute Physiology and Chronic Health Evaluation II scores had low accuracy in prediction of short-term death.ICU admitted esophageal cancer patients have poor prognosis,especially those with acute illness.CONCLUSION The prognostic tools for these patients need to be further optimized.
文摘BACKGROUND Colorectal cancer(CRC)is one of the most common cancers and CRC patients are among the most common intensive care unit(ICU)admitted cancer patients.However,their prognosis and evaluation methods are rarely studied.AIM To determine the short-term mortality outcome and identify the potential prognostic factors of CRC cancer patients admitted to the ICU.METHODS A multicenter cross-sectional study was performed from May 10,2021 to July 10,2021 at the ICU departments of 37 cancer specialized hospitals in China,and included patients aged≥14 years with ICU duration≥24 hours.Clinical records of patients with a primary CRC diagnosis were reviewed.Patients were separated into groups according to 90-day survival.Characteristics between groups were compared.Univariate and multivariate regression tests were used to analyze the correlated factors of ICU outcomes.Predictive values of disease severity scores were assessed using receiver operating characteristic curve analysis.RESULTS In total,189 CRC patients were included in the study.The 90-day mortality was 12.2%.Patients who died showed differences compared to patients who survived mostly in terms of disease severity and ICU complications.It appears that patients admitted to the ICU from a clinical ward due to emergencies may have a higher risk of mortality while surgical management was associated with better survival.In multivariate analysis,only chemotherapy,elective surgery and conventional oxygen therapy were identified as independently correlated with 90-day mortality.Sequential organ failure assessment and acute physiology and chronic health evaluation II scores had moderate accuracy in predicting short-term mortality.CONCLUSION ICU admitted CRC patients appear to have low short-term mortality which requires further confirmation in prospective studies.The prognostic tools for these patients need further optimization.
基金the funding information for the Zhejiang Provincial Natural Science Foundation of China(No.LBY21H160001)was wrongThe correct funding should be the Zhejiang Health Science and Technology Project(No.2022KY682),China.
基金supported by the Zhejiang Provincial Natural Science Foundation of China(No.LBY21H160001).
文摘Breast cancer is a malignant tumor that seriously endangers women's lives. The prognosis of breast cancer patients differs among molecular types. Compared with other subtypes, triple-negative breast cancer(TNBC) has been a research hotspot in recent years because of its high degree of malignancy, strong invasiveness, rapid progression, easy of recurrence,distant metastasis, poor prognosis, and high mortality. Many studies have found that long non-coding RNA(lncRNA) plays an important role in the occurrence, proliferation, migration, recurrence, chemotherapy resistance, and other characteristics of TNBC. Some lncRNAs are expected to become biomarkers in the diagnosis and prognosis of TNBC, and even new targets for its treatment. Based on a PubMed literature search, this review summarizes the progress in research on lncRNAs in TNBC and discusses their roles in TNBC diagnosis, prognosis, and chemotherapy with the hope of providing help for future research.
