To purify Methamidophos (Met) monoclonal antibodies with two methods and compare immune activity of purified antibodies. Method Caprylic acid ammonium sulphate precipition (CAASP) method and Sepharose protein-A (S...To purify Methamidophos (Met) monoclonal antibodies with two methods and compare immune activity of purified antibodies. Method Caprylic acid ammonium sulphate precipition (CAASP) method and Sepharose protein-A (SPA) affinity chromatography method were used to purify Met monoclonal antibodies, UV spectrum scanning was used to determine protein content and recovery of purified antibodies, sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the purity of purified antibodies, and enzyme-linked immunosorbent assay (ELISA) was used to determine immune activity of purified antibodies. Results Antibody protein content and recovery rate with CAASP method were 7.62 mg/mL and 8.05% respectively, antibody protein content and recovery rate with SPA method were 6.45 mg/mL and 5.52% respectively. Purity of antibodies purified by SPA method was higher than that by CAASP method. The half-maximal inhibition concentration (IC50) of antibodies purified by SPA to Met was 181.26 靏/mL, and the linear working range and the limit of quantification (LOD) were 2.43-3896.01 靏/mL and 1.03 靏/mL, respectively. The IC50 of antibodies purified by CAASP to Met was 352.82 靏/mL, and the linear working range and LOD were 10.91-11412.29 靏/mL and 3.42 靏/mL, respectively. Conclusion Antibodies purified by SPA method are better than those by CAASP method, and Met monoclonal antibodies purified by SPA method can be used to prepare gold-labelled testing paper for analyzing Met residue in vegetable and drink water.展开更多
The effect of applying biological organic fertilizer(BOF)on bacterial wilt incidence of tomato and soil microbial community under continuous cropping was studied. The results showed that all the tomatoes were infected...The effect of applying biological organic fertilizer(BOF)on bacterial wilt incidence of tomato and soil microbial community under continuous cropping was studied. The results showed that all the tomatoes were infected by bacterial wilt in the control. The infection rates of tomatoes in the treatments with un-composted BOF and decomposed BOF were 55 and 50% respectively. Fatty acid methyl esters(FAME)analysis indicated soil microbial community changed sensitively after applying BOF. Soil FAME total content, relative fungi content and ratio of fungi to bacteria were significantly increased in both BOF treatments. The soil odd-number fatty acid proportion changed after applying BOF, aC15 : 0, iC17 : 0 decreased, while cyC17 : 0 increased in soil odd-number fatty acid proportion. BOF application would strengthen soil health and disease suppression. The content of C16 : 1 11c in soil microbial community was obviously increased after decomposed BOF application. It indicated that the growth of AM fungi could be enhanced with decomposed BOF application. FAME microbial biomarkers could be used for an indicator of soil health and disease suppression. Odd-number fatty acid proportion was a sensitive indicator of the effect of applying un-composted and decomposed BOF respectively on soil health regulating.展开更多
基金National Natural Science Foundation of China (39970519) Guangxi Basic Studying Fund (0236053) +1 种基金 Guangdong Province Natural Science Foundation (990683) Core Teacher Project of Ministry of Education and Chinese Postdoctoral Fu
文摘To purify Methamidophos (Met) monoclonal antibodies with two methods and compare immune activity of purified antibodies. Method Caprylic acid ammonium sulphate precipition (CAASP) method and Sepharose protein-A (SPA) affinity chromatography method were used to purify Met monoclonal antibodies, UV spectrum scanning was used to determine protein content and recovery of purified antibodies, sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the purity of purified antibodies, and enzyme-linked immunosorbent assay (ELISA) was used to determine immune activity of purified antibodies. Results Antibody protein content and recovery rate with CAASP method were 7.62 mg/mL and 8.05% respectively, antibody protein content and recovery rate with SPA method were 6.45 mg/mL and 5.52% respectively. Purity of antibodies purified by SPA method was higher than that by CAASP method. The half-maximal inhibition concentration (IC50) of antibodies purified by SPA to Met was 181.26 靏/mL, and the linear working range and the limit of quantification (LOD) were 2.43-3896.01 靏/mL and 1.03 靏/mL, respectively. The IC50 of antibodies purified by CAASP to Met was 352.82 靏/mL, and the linear working range and LOD were 10.91-11412.29 靏/mL and 3.42 靏/mL, respectively. Conclusion Antibodies purified by SPA method are better than those by CAASP method, and Met monoclonal antibodies purified by SPA method can be used to prepare gold-labelled testing paper for analyzing Met residue in vegetable and drink water.
基金supported by Guangxi Zhuang Autonomous Region Youth Fund(00007019)National 863 Program,China(2001AA246023)
文摘The effect of applying biological organic fertilizer(BOF)on bacterial wilt incidence of tomato and soil microbial community under continuous cropping was studied. The results showed that all the tomatoes were infected by bacterial wilt in the control. The infection rates of tomatoes in the treatments with un-composted BOF and decomposed BOF were 55 and 50% respectively. Fatty acid methyl esters(FAME)analysis indicated soil microbial community changed sensitively after applying BOF. Soil FAME total content, relative fungi content and ratio of fungi to bacteria were significantly increased in both BOF treatments. The soil odd-number fatty acid proportion changed after applying BOF, aC15 : 0, iC17 : 0 decreased, while cyC17 : 0 increased in soil odd-number fatty acid proportion. BOF application would strengthen soil health and disease suppression. The content of C16 : 1 11c in soil microbial community was obviously increased after decomposed BOF application. It indicated that the growth of AM fungi could be enhanced with decomposed BOF application. FAME microbial biomarkers could be used for an indicator of soil health and disease suppression. Odd-number fatty acid proportion was a sensitive indicator of the effect of applying un-composted and decomposed BOF respectively on soil health regulating.
