Recently, approaches utilizing spatial-temporal features to form Bag-of-Words （BoWs） models have achieved great success due to their simplicity and effectiveness. But they still have difficulties when distinguishing...Recently, approaches utilizing spatial-temporal features to form Bag-of-Words （BoWs） models have achieved great success due to their simplicity and effectiveness. But they still have difficulties when distinguishing between actions with high inter-ambiguity. The main reason is that they describe actions by orderless bag of features, and ignore the spatial and temporal structure information of visual words. In order to improve classification performance, we present a novel approach called sequential Bag-of-Words. It captures temporal sequential structure by segmenting the entire action into sub-actions. Meanwhile, we pay more attention to the distinguishing parts of an action by classifying sub- actions separately, which is then employed to vote for the final result. Extensive experiments are conducted on challenging datasets and real scenes to evaluate our method. Concretely, we compare our results to some state-of-the-art classification approaches and confirm the advantages of our approach to distinguish similar actions. Results show that our approach is robust and outperforms most existing BoWs based classification approaches, especially on complex datasets with interactive activities, cluttered backgrounds and inter-class action ambiguities.展开更多
Objective To investigate the effects and underlying mechanisms of Panax quinquefolium saponin(PQS)on energy deficiency in hypoxia-reperfusion(H/R)induced cardiomyocytes.Methods The H/R injury involved hypoxia for 3 h ...Objective To investigate the effects and underlying mechanisms of Panax quinquefolium saponin(PQS)on energy deficiency in hypoxia-reperfusion(H/R)induced cardiomyocytes.Methods The H/R injury involved hypoxia for 3 h and then reperfusion for 2 h.Cardiomyocytes recruited from neonatal rat ventricular myocytes(NRVMs)were randomly divided into control,H/R,H/R+compound C(C.C),H/R+PQS,and H/R+C.C+PQS groups.BrdU assay,lactase dehydrogenase(LDH)leakage and early apoptosis rate were evaluated to assess cell damages.Contents of high energy phosphate compounds were conducted to detect the energy production.Protein expression levels of adenosine monophosphate-activated protein kinase a(AMPKα),glucose transporter 4(GLUT4),phosphate fructose kinase 2(PFK2),fatty acid translocase/cluster of differentiation 36(FAT/CD36),and acetyl CoA carboxylase 2(ACC2)in the regulatory pathways were measured by Western blotting.Immunofluorescence staining of GLUT4 and FAT/CD36 was used to observe the mobilization of metabolic transporters.Results PQS(50 mg/L)pretreatment significantly alleviated H/R-induced inhibition of NRVMs viability,up-regulation of LDH leakage,acceleration of early apoptosis,and reduction of energy production(P<0.05).Compared with the H/R group,up-regulated expression of AMPKα,GLUT4,PFK2,FAT/CD36 and ACC2 were observed,and more GLUT4 and FAT/CD36 expressions were detected on the membrane in the H/R+PQS group(P<0.05).These effects of PQS on H/R-induced NRVMs were eliminated in the H/R+C.C+PQS group(P<0.05).Conclusion PQS has prominent advantages in protecting NRVMs from H/R-induced cell damages and energy metabolic disorders,by activation of AMPKα-mediated GLUT4-PFK2 and FAT/CD36-ACC2 pathways.展开更多
文摘Recently, approaches utilizing spatial-temporal features to form Bag-of-Words （BoWs） models have achieved great success due to their simplicity and effectiveness. But they still have difficulties when distinguishing between actions with high inter-ambiguity. The main reason is that they describe actions by orderless bag of features, and ignore the spatial and temporal structure information of visual words. In order to improve classification performance, we present a novel approach called sequential Bag-of-Words. It captures temporal sequential structure by segmenting the entire action into sub-actions. Meanwhile, we pay more attention to the distinguishing parts of an action by classifying sub- actions separately, which is then employed to vote for the final result. Extensive experiments are conducted on challenging datasets and real scenes to evaluate our method. Concretely, we compare our results to some state-of-the-art classification approaches and confirm the advantages of our approach to distinguish similar actions. Results show that our approach is robust and outperforms most existing BoWs based classification approaches, especially on complex datasets with interactive activities, cluttered backgrounds and inter-class action ambiguities.
基金Supported by the National Natural Science Foundation of China(No.81273934 and No.81874410)。
文摘Objective To investigate the effects and underlying mechanisms of Panax quinquefolium saponin(PQS)on energy deficiency in hypoxia-reperfusion(H/R)induced cardiomyocytes.Methods The H/R injury involved hypoxia for 3 h and then reperfusion for 2 h.Cardiomyocytes recruited from neonatal rat ventricular myocytes(NRVMs)were randomly divided into control,H/R,H/R+compound C(C.C),H/R+PQS,and H/R+C.C+PQS groups.BrdU assay,lactase dehydrogenase(LDH)leakage and early apoptosis rate were evaluated to assess cell damages.Contents of high energy phosphate compounds were conducted to detect the energy production.Protein expression levels of adenosine monophosphate-activated protein kinase a(AMPKα),glucose transporter 4(GLUT4),phosphate fructose kinase 2(PFK2),fatty acid translocase/cluster of differentiation 36(FAT/CD36),and acetyl CoA carboxylase 2(ACC2)in the regulatory pathways were measured by Western blotting.Immunofluorescence staining of GLUT4 and FAT/CD36 was used to observe the mobilization of metabolic transporters.Results PQS(50 mg/L)pretreatment significantly alleviated H/R-induced inhibition of NRVMs viability,up-regulation of LDH leakage,acceleration of early apoptosis,and reduction of energy production(P<0.05).Compared with the H/R group,up-regulated expression of AMPKα,GLUT4,PFK2,FAT/CD36 and ACC2 were observed,and more GLUT4 and FAT/CD36 expressions were detected on the membrane in the H/R+PQS group(P<0.05).These effects of PQS on H/R-induced NRVMs were eliminated in the H/R+C.C+PQS group(P<0.05).Conclusion PQS has prominent advantages in protecting NRVMs from H/R-induced cell damages and energy metabolic disorders,by activation of AMPKα-mediated GLUT4-PFK2 and FAT/CD36-ACC2 pathways.
