Fusarium ear rot(FER)caused by Fusarium species severely reduces grain yield and quality of maize.Genome prediction(GP),a promising tool for quantitative trait breeding in plants and animals,uses molecular markers for...Fusarium ear rot(FER)caused by Fusarium species severely reduces grain yield and quality of maize.Genome prediction(GP),a promising tool for quantitative trait breeding in plants and animals,uses molecular markers for capturing quantitative trait loci and predicting the genetic value of candidates for selection.In the present study,different subsets of markers and statistical methods for GP accuracy were tested in diverse inbred populations for FER resistance using a five-fold cross-validation approach.The prediction accuracy increased with an increase in the number of random markers;however,an increase in number beyond 10K did not increase the prediction accuracy.The prediction accuracy of selected markers was higher than that of random markers,and 500–1000 selected markers had the highest prediction accuracy,beyond which it slowly decreased.Although there was no difference among statistical methods when using selected markers at high prediction accuracy,significant differences were observed when using random markers.On this basis,a liquid chip named FER0.4K(liquid chip for genomic prediction of FER)containing 381 SNPs was developed for low-cost,high-throughput genotyping,with a prediction of approximately 0.82.The statistical method of genome prediction was compiled into a web-based,easy-to-use statistical analysis software using the‘‘shiny”package in R.In summary,this study provides a foundation for FER resistance breeding in maize and offers new insights into the genetic improvement of other complex quantitative traits in plants.展开更多
Chemical communication in plant–microbiome and intra-microbiome interactions weaves a complex network,critically shaping ecosystem stability and agricultural productivity.This non-contact interaction is driven by sma...Chemical communication in plant–microbiome and intra-microbiome interactions weaves a complex network,critically shaping ecosystem stability and agricultural productivity.This non-contact interaction is driven by small-molecule signals that orchestrate crosstalk dynamics and beneficial association.Plants leverage these signals to distinguish between pathogens and beneficial microbes,dynamically modulate immune responses,and secrete exudates to recruit a beneficial microbiome,while microbes in turn influence plant nutrient acquisition and stress resilience.Such bidirectional chemical dialogues underpin nutrient cycling,co-evolution,microbiome assembly,and plant resistance.However,knowledge gaps persist regarding validating the key molecules involved in plant–microbe interactions.Interpreting chemical communication requires multi-omics integration to predict key information,genome editing and click chemistry to verify the function of biomolecules,and artificial intelligence(AI)models to improve resolution and accuracy.This review helps advance the understanding of chemical communication and provides theoretical support for agriculture to cope with food insecurity and climate challenges.展开更多
1 The need to extend and expand the maternal,neonatal and child health (MNCH) continuum to include preconception health The provision of care along the MNCH continuum is currently the core strategy for reducing m...1 The need to extend and expand the maternal,neonatal and child health (MNCH) continuum to include preconception health The provision of care along the MNCH continuum is currently the core strategy for reducing maternal,neonatal and infant mortality [1-5]. Yet,gaps in the MNCH continuum persist. Reductions in maternal mortality have stagnated in many parts of the world. With the lowering of infant and child mortality,approximately 40% of infant deaths now occur in the neonatal period [6]. Strong scientific evidence indicates that improving women's health before pregnancy would result in better maternal and neonatal health outcomes-specifically,fewer pregnancy complications and a reduction in rates of birth defects,fetal loss,low birth weight and preterm delivery [7]. The WHO estimates that 19% of all maternal deaths are due indirectly to preexisting medical conditions [8]. Furthermore,early prenatal care is too late to prevent many adverse birth outcomes,as organogenesis is completed during the critical first weeks of pregnancy,often before a woman knows that she is pregnant [9]. There is a clear need to strengthen preconception health services.展开更多
AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cel...AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cell line(Raw 264.7),mouse primary antigen-presenting cells(APCs,MHCII+) and CD11c+dendritic cells to analyze the effects of cinnamon extract on APC function.The mechanisms of action of cinnamon extract on APCs were investigated by analyzing cytokine production,and expression of MHC antigens and co-stimulatory molecules by quantitative real-time PCR and flow cytometry.In addition,the effect of cinnamon extract on antigen presentation capacity and APC-dependent T-cell differentiation were analyzed by [H3]-thymidine incorporation and cytokine analysis,respectively.To confirm the anti-inflammatory effects of cinnamon extract in vivo,cinnamon or PBS was orally administered to mice for 20 d followed by induction of experimental colitis with 2,4,6 trinitrobenzenesulfonic acid.The protective effects of cinnamon extract against experimental colitis were measured by checking clinical symptoms,histological analysis and cytokine expression prof iles in inflamed tissue.RESULTS:Treatment with cinnamon extract inhibited maturation of MHCII+ APCs or CD11c+ dendritic cells(DCs) by suppressing expression of co-stimulatory molecules(B7.1,B7.2,ICOS-L),MHCII and cyclooxygenase(COX)-2.Cinnamon extract induced regulatory DCs(rDCs) that produce low levels of pro-inflammatory cytokines [interleukin(IL)-1β,IL-6,IL-12,interferon(IFN)-γ and tumor necrosis factor(TNF)-α] while expressing high levels of immunoregulatory cytokines(IL-10 and transforming growth factor-β).In addition,rDCs generated by cinnamon extract inhibited APC-dependent T-cell proliferation,and converted CD4+ T cells into IL-10high CD4+ T cells.Furthermore,oral administration of cinnamon extract inhibited development and progression of intestinal colitis by inhibiting expression of COX-2 and pro-inflammatory cytokines(IL-1β,IFN-γ and TNF-α),while enhancing IL-10 levels.CONCLUSION:Our study suggests the potential of cinnamon extract as an anti-inflammatory agent by targeting the generation of regulatory APCs and IL-10+ regulatory T cells.展开更多
Oxidative stress is one of the major causes of male infertility; it damages spermatogenic cells, the spermatogenic process and sperm function. Recent advances in redox biology have revealed the signalling role of reac...Oxidative stress is one of the major causes of male infertility; it damages spermatogenic cells, the spermatogenic process and sperm function. Recent advances in redox biology have revealed the signalling role of reactive oxygen species （ROS） that are generated by cells. While highly reactive oxidants, such as the hydroxyl radical, exert largely deleterious effects, hydrogen peroxide can feasibly serve as a signal mediator because it is moderately reactive and membrane permeable and because it can oxidize only limited numbers of functional groups of biological molecules. The amino acid side chain most sensitive to oxidation is cysteine sulphydryl, which is commonly involved in the catalysis of some enzymes. Although the reactivity of cysteine sulphhydryl is not very high in ordinary proteins, some phosphatases possess a highly reactive sulphydryl group at their catalytic centre and are thereby oxidatively inactivated by transiently elevated hydrogen peroxide levels after extracellular stimuli and under certain environmental conditions. Peroxiredoxins, in turn, show moderate hydrogen peroxide-reducing activity, and their role in the modulation of ROS-mediated signal transduction in ordinary cells, mediated by protecting phosphatases from oxidative inactivation, has attracted much attention. Although knowledge of the signalling role of ROS in the male reproductive system is limited at present, its significance is becoming a focal issue. Here, we present a review of the emerging signalling role of hydrogen peroxide in testes.展开更多
Background: Edge effects cause changes in bird community richness, abundance, and/or distribution within a landscape, but the avian guilds most influenced can vary among regions. Although Southeast Asia has the highes...Background: Edge effects cause changes in bird community richness, abundance, and/or distribution within a landscape, but the avian guilds most influenced can vary among regions. Although Southeast Asia has the highest rates of deforestation and projected species loss, and is currently undergoing an explosive growth in road infrastructure, there have been few studies of the effects of forest edges on avian communities in this region.Methods: We examined avian community structure in a dry evergreen forest in northeastern Thailand adjacent to a five-lane highway. We evaluated the richness and abundance of birds in 11 guilds at 24 survey points on three parallel transects perpendicular to the edge. At each point, 10-min surveys were conducted during February-August 2014 and March-August 2015. Vegetation measurements were conducted at 16 of the bird survey points and ambient noise was measured at all 24 survey points.Results: We found a strongly negative response to the forest edge for bark-gleaning, sallying, terrestrial, and understory insectivores and a weakly negative response for arboreal frugivore-insectivores, foliage gleaning insectivores, and raptors. Densities of trees and the percentage canopy cover were higher in the interior, and the ambient noise was lower. In contrast, arboreal nectarivore-insectivores responded positively to the forest edge, where there was a higher vegetation cover in the ground layer, a lower tree density, and a higher level of ambient noise.Conclusion: Planners should avoid road development in forests of high conservation value to reduce impacts on biodiversity. Where avoidance is impossible, a number of potential mitigation methods are available, but more detailed assessments of these are needed before they are applied in this region.展开更多
Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well understood.In this study,GWAS performed by MLM and 3VmrMLM identi...Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well understood.In this study,GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs,associated with seedling blight resistance.These methods identified 49 and 36 genes,respectively.Functional verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with F.verticillioides,compared with the WT.The length of the stem rot lesion caused by F.verticillioides increased by 86%in mutant seedlings,and the relative length of the adult plant stalk rot increased by 35%in mutant plants compared to the wild type after inoculation with Fusarium graminearum.Transcriptome analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type,synthesis of secondary metabolites associated with resistance was reduced,and the immune response triggered by PAMP decreased,resulting in decreased resistance of mutant maize seedlings.Candidate gene association analysis showed that most maize inbred lines carried the susceptible haplotype.A functional PCR marker was developed.The results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.展开更多
Groundwater is the main source of water in the semi-arid Calera watershed, located in the State of Zacatecas, Mexico. Due to increasing population, rapid industrial growth, and increased irrigation to meet growing foo...Groundwater is the main source of water in the semi-arid Calera watershed, located in the State of Zacatecas, Mexico. Due to increasing population, rapid industrial growth, and increased irrigation to meet growing food demand, groundwater extraction in the Calera watershed are exceeding recharge rates. Therefore, development and evaluation of alter-native water management strategies are needed for sustainable development of the region. The Soil and Water Assessment Tool (SWAT) model was selected for this purpose as it has been used to simulate a wide range of agricultural production, the extensive testing and application in diverse watersheds worldwide, and the potential for future linkage of this model to groundwater models. However, crucial flow data which are commonly used for calibrating hydrologic models are not available in this watershed. This paper describes a novel calibration methodology that uses biomass and water balance approach which has potential for calibration of hydrologic models in ungauged or data-scarce watersheds, which are prevalent in many parts of the world. Estimated long-term annual average actual evapotranspiration (AET), and deep aquifer recharge rates and plant biomass values based on the expert knowledge of researchers and managers in the watershed were used as targets for calibration. The model performance was assessed using the Nash-Sutcliffe effi-ciency coefficient (NSE), coefficient of determination (R2), and percent bias (PBIAS, %) statistics. On average, the calibrated SWAT model yielded annual Nash-Sutcliffe efficiency coefficient values of 0.95, 0.99, and 0.85 for AET, recharge, and biomass, respectively. The coefficient of determination, values for AET, recharge, and biomass were 0.95, 0.94, and 0.99 respectively. The percent bias values of ±2.21%, ±0.18%, and ±0.96% for AET, recharge, and biomass, respectively, indicated that the model reproduced the calibration target values of the three water budget variables within an acceptable value of ± 10.0%. Therefore, it is concluded that the calibrated SWAT model can be used in evaluating alternative water management scenarios for the Calera watershed without further validation. Considering the relative ease in developing calibration data and excellent performance statistics, the calibration methodology proposed in this study may have the potential to be used for ungauged or data-scare agricultural watersheds that are prevalent in many parts of the world.展开更多
基金supported by National Natural Science Foundation of Henan(212300410046)the Henan Innovative Talent Project(22HASTIT040)Key Project for Research and Practice on Higher Education Teaching Reform at Henan Agricultural University(2024XJGLX031)。
文摘Fusarium ear rot(FER)caused by Fusarium species severely reduces grain yield and quality of maize.Genome prediction(GP),a promising tool for quantitative trait breeding in plants and animals,uses molecular markers for capturing quantitative trait loci and predicting the genetic value of candidates for selection.In the present study,different subsets of markers and statistical methods for GP accuracy were tested in diverse inbred populations for FER resistance using a five-fold cross-validation approach.The prediction accuracy increased with an increase in the number of random markers;however,an increase in number beyond 10K did not increase the prediction accuracy.The prediction accuracy of selected markers was higher than that of random markers,and 500–1000 selected markers had the highest prediction accuracy,beyond which it slowly decreased.Although there was no difference among statistical methods when using selected markers at high prediction accuracy,significant differences were observed when using random markers.On this basis,a liquid chip named FER0.4K(liquid chip for genomic prediction of FER)containing 381 SNPs was developed for low-cost,high-throughput genotyping,with a prediction of approximately 0.82.The statistical method of genome prediction was compiled into a web-based,easy-to-use statistical analysis software using the‘‘shiny”package in R.In summary,this study provides a foundation for FER resistance breeding in maize and offers new insights into the genetic improvement of other complex quantitative traits in plants.
基金supported by the National Key R&D Program of China(No.2025YFE0104500)the Zhejiang Provincial Natural Science Foundation of China(No.LD25C140002),the Natural Science Foundation of Hangzhou(No.2024SZRZDC 130001)+1 种基金the National Natural Science Foundation of China(Nos.U21A20219 and 32122074)the Zhejiang University Global Partnership Fund,China.
文摘Chemical communication in plant–microbiome and intra-microbiome interactions weaves a complex network,critically shaping ecosystem stability and agricultural productivity.This non-contact interaction is driven by small-molecule signals that orchestrate crosstalk dynamics and beneficial association.Plants leverage these signals to distinguish between pathogens and beneficial microbes,dynamically modulate immune responses,and secrete exudates to recruit a beneficial microbiome,while microbes in turn influence plant nutrient acquisition and stress resilience.Such bidirectional chemical dialogues underpin nutrient cycling,co-evolution,microbiome assembly,and plant resistance.However,knowledge gaps persist regarding validating the key molecules involved in plant–microbe interactions.Interpreting chemical communication requires multi-omics integration to predict key information,genome editing and click chemistry to verify the function of biomolecules,and artificial intelligence(AI)models to improve resolution and accuracy.This review helps advance the understanding of chemical communication and provides theoretical support for agriculture to cope with food insecurity and climate challenges.
基金北京林业大学研究生自选课题基金资助项目(06jj038)中美碳联盟USCCC国际合作资助项目美国宇航局项目“Effects of Land Use Change on The Energy and Water Balance of The Semi-Arid Region of Inner Mongolia”
文摘1 The need to extend and expand the maternal,neonatal and child health (MNCH) continuum to include preconception health The provision of care along the MNCH continuum is currently the core strategy for reducing maternal,neonatal and infant mortality [1-5]. Yet,gaps in the MNCH continuum persist. Reductions in maternal mortality have stagnated in many parts of the world. With the lowering of infant and child mortality,approximately 40% of infant deaths now occur in the neonatal period [6]. Strong scientific evidence indicates that improving women's health before pregnancy would result in better maternal and neonatal health outcomes-specifically,fewer pregnancy complications and a reduction in rates of birth defects,fetal loss,low birth weight and preterm delivery [7]. The WHO estimates that 19% of all maternal deaths are due indirectly to preexisting medical conditions [8]. Furthermore,early prenatal care is too late to prevent many adverse birth outcomes,as organogenesis is completed during the critical first weeks of pregnancy,often before a woman knows that she is pregnant [9]. There is a clear need to strengthen preconception health services.
基金Supported by Grants from the BioGreen 21 Program, Rural Development Administration (PJ007054)Regional Technology Innovation Program of the MOCIE (RTI05-01-01)Korea Healthcare Technology R&D Project, Ministry of Health and Welfare (A080588-20)
文摘AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting cells.METHODS:Cinnamon extract was used to treat murine macrophage cell line(Raw 264.7),mouse primary antigen-presenting cells(APCs,MHCII+) and CD11c+dendritic cells to analyze the effects of cinnamon extract on APC function.The mechanisms of action of cinnamon extract on APCs were investigated by analyzing cytokine production,and expression of MHC antigens and co-stimulatory molecules by quantitative real-time PCR and flow cytometry.In addition,the effect of cinnamon extract on antigen presentation capacity and APC-dependent T-cell differentiation were analyzed by [H3]-thymidine incorporation and cytokine analysis,respectively.To confirm the anti-inflammatory effects of cinnamon extract in vivo,cinnamon or PBS was orally administered to mice for 20 d followed by induction of experimental colitis with 2,4,6 trinitrobenzenesulfonic acid.The protective effects of cinnamon extract against experimental colitis were measured by checking clinical symptoms,histological analysis and cytokine expression prof iles in inflamed tissue.RESULTS:Treatment with cinnamon extract inhibited maturation of MHCII+ APCs or CD11c+ dendritic cells(DCs) by suppressing expression of co-stimulatory molecules(B7.1,B7.2,ICOS-L),MHCII and cyclooxygenase(COX)-2.Cinnamon extract induced regulatory DCs(rDCs) that produce low levels of pro-inflammatory cytokines [interleukin(IL)-1β,IL-6,IL-12,interferon(IFN)-γ and tumor necrosis factor(TNF)-α] while expressing high levels of immunoregulatory cytokines(IL-10 and transforming growth factor-β).In addition,rDCs generated by cinnamon extract inhibited APC-dependent T-cell proliferation,and converted CD4+ T cells into IL-10high CD4+ T cells.Furthermore,oral administration of cinnamon extract inhibited development and progression of intestinal colitis by inhibiting expression of COX-2 and pro-inflammatory cytokines(IL-1β,IFN-γ and TNF-α),while enhancing IL-10 levels.CONCLUSION:Our study suggests the potential of cinnamon extract as an anti-inflammatory agent by targeting the generation of regulatory APCs and IL-10+ regulatory T cells.
文摘Oxidative stress is one of the major causes of male infertility; it damages spermatogenic cells, the spermatogenic process and sperm function. Recent advances in redox biology have revealed the signalling role of reactive oxygen species （ROS） that are generated by cells. While highly reactive oxidants, such as the hydroxyl radical, exert largely deleterious effects, hydrogen peroxide can feasibly serve as a signal mediator because it is moderately reactive and membrane permeable and because it can oxidize only limited numbers of functional groups of biological molecules. The amino acid side chain most sensitive to oxidation is cysteine sulphydryl, which is commonly involved in the catalysis of some enzymes. Although the reactivity of cysteine sulphhydryl is not very high in ordinary proteins, some phosphatases possess a highly reactive sulphydryl group at their catalytic centre and are thereby oxidatively inactivated by transiently elevated hydrogen peroxide levels after extracellular stimuli and under certain environmental conditions. Peroxiredoxins, in turn, show moderate hydrogen peroxide-reducing activity, and their role in the modulation of ROS-mediated signal transduction in ordinary cells, mediated by protecting phosphatases from oxidative inactivation, has attracted much attention. Although knowledge of the signalling role of ROS in the male reproductive system is limited at present, its significance is becoming a focal issue. Here, we present a review of the emerging signalling role of hydrogen peroxide in testes.
基金supported by King Mongkut’s University of Technology Thonburi(Thailand)the National Science and Technology Development Agency(CPMO P-14-51347)supported by the Royal Golden Jubilee Ph.D.Program,Thailand(PHD/0036/2556)
文摘Background: Edge effects cause changes in bird community richness, abundance, and/or distribution within a landscape, but the avian guilds most influenced can vary among regions. Although Southeast Asia has the highest rates of deforestation and projected species loss, and is currently undergoing an explosive growth in road infrastructure, there have been few studies of the effects of forest edges on avian communities in this region.Methods: We examined avian community structure in a dry evergreen forest in northeastern Thailand adjacent to a five-lane highway. We evaluated the richness and abundance of birds in 11 guilds at 24 survey points on three parallel transects perpendicular to the edge. At each point, 10-min surveys were conducted during February-August 2014 and March-August 2015. Vegetation measurements were conducted at 16 of the bird survey points and ambient noise was measured at all 24 survey points.Results: We found a strongly negative response to the forest edge for bark-gleaning, sallying, terrestrial, and understory insectivores and a weakly negative response for arboreal frugivore-insectivores, foliage gleaning insectivores, and raptors. Densities of trees and the percentage canopy cover were higher in the interior, and the ambient noise was lower. In contrast, arboreal nectarivore-insectivores responded positively to the forest edge, where there was a higher vegetation cover in the ground layer, a lower tree density, and a higher level of ambient noise.Conclusion: Planners should avoid road development in forests of high conservation value to reduce impacts on biodiversity. Where avoidance is impossible, a number of potential mitigation methods are available, but more detailed assessments of these are needed before they are applied in this region.
基金supported by grants from the National Key Research and Development Program Project of China(2022YFD1201803)Research on Resistance Genetics of Maize Root Rot Disease,State Key Laboratory of Agronomy College,Henan Agricultural University,China(39990073/111)。
文摘Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well understood.In this study,GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs,associated with seedling blight resistance.These methods identified 49 and 36 genes,respectively.Functional verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with F.verticillioides,compared with the WT.The length of the stem rot lesion caused by F.verticillioides increased by 86%in mutant seedlings,and the relative length of the adult plant stalk rot increased by 35%in mutant plants compared to the wild type after inoculation with Fusarium graminearum.Transcriptome analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type,synthesis of secondary metabolites associated with resistance was reduced,and the immune response triggered by PAMP decreased,resulting in decreased resistance of mutant maize seedlings.Candidate gene association analysis showed that most maize inbred lines carried the susceptible haplotype.A functional PCR marker was developed.The results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.
文摘Groundwater is the main source of water in the semi-arid Calera watershed, located in the State of Zacatecas, Mexico. Due to increasing population, rapid industrial growth, and increased irrigation to meet growing food demand, groundwater extraction in the Calera watershed are exceeding recharge rates. Therefore, development and evaluation of alter-native water management strategies are needed for sustainable development of the region. The Soil and Water Assessment Tool (SWAT) model was selected for this purpose as it has been used to simulate a wide range of agricultural production, the extensive testing and application in diverse watersheds worldwide, and the potential for future linkage of this model to groundwater models. However, crucial flow data which are commonly used for calibrating hydrologic models are not available in this watershed. This paper describes a novel calibration methodology that uses biomass and water balance approach which has potential for calibration of hydrologic models in ungauged or data-scarce watersheds, which are prevalent in many parts of the world. Estimated long-term annual average actual evapotranspiration (AET), and deep aquifer recharge rates and plant biomass values based on the expert knowledge of researchers and managers in the watershed were used as targets for calibration. The model performance was assessed using the Nash-Sutcliffe effi-ciency coefficient (NSE), coefficient of determination (R2), and percent bias (PBIAS, %) statistics. On average, the calibrated SWAT model yielded annual Nash-Sutcliffe efficiency coefficient values of 0.95, 0.99, and 0.85 for AET, recharge, and biomass, respectively. The coefficient of determination, values for AET, recharge, and biomass were 0.95, 0.94, and 0.99 respectively. The percent bias values of ±2.21%, ±0.18%, and ±0.96% for AET, recharge, and biomass, respectively, indicated that the model reproduced the calibration target values of the three water budget variables within an acceptable value of ± 10.0%. Therefore, it is concluded that the calibrated SWAT model can be used in evaluating alternative water management scenarios for the Calera watershed without further validation. Considering the relative ease in developing calibration data and excellent performance statistics, the calibration methodology proposed in this study may have the potential to be used for ungauged or data-scare agricultural watersheds that are prevalent in many parts of the world.
