BACKGROUND Gastric subepithelial lesions(SELs)are elevated lesions originating from the muscularis mucosa,submucosa,or muscularis propria,and may also include extraluminal lesions.For small SELs(less than 5 cm),comple...BACKGROUND Gastric subepithelial lesions(SELs)are elevated lesions originating from the muscularis mucosa,submucosa,or muscularis propria,and may also include extraluminal lesions.For small SELs(less than 5 cm),complete endoscopic excision is the preferred treatment.Endoscopic full-thickness resection(EFTR)has proven to be an effective approach.AIM To evaluate the efficacy of the interrupted closure technique compared to the traditional closure technique in EFTR for gastric SELs.METHODS This single-center,prospective,randomized controlled trial was conducted at a tertiary hospital from September 2023 to September 2024.A total of 90 patients who underwent EFTR for gastric SELs were randomly allocated to either the interrupted closure group(n=44)or the traditional closure group(n=46).RESULTS All patients had complete resection and wound closure without any severe postoperative complications.The incidence of intraoperative gas-related complications was significantly lower in the interrupted closure group than in the traditional closure group(2.27%vs 26.09%,P=0.001),demonstrating interrupted closure technique can reduce the incidence of gas-related issues.Statistical analysis revealed that the incidence of postoperative infection was significantly lower in the experimental group than in the control group(15.91%vs 41.30%,P=0.008).Additionally,the median duration of antibiotic use was lower in the experimental group(3.5 days vs 5 days,P=0.013).Abdominal pain levels on postoperative days 1 and 4 were also lower in the experimental group compared to the control group(P<0.001).CONCLUSION The interrupted closure technique in EFTR for treating gastric SELs is safe and effective,reducing the incidence of intraoperative gas complications and postoperative infections.展开更多
BACKGROUND Ulcerative colitis(UC)is an inflammatory condition with frequent relapse and recurrence.Evidence suggests the involvement of SLC6A14 in UC pathogenesis,but the central regulator remains unknown.AIM To explo...BACKGROUND Ulcerative colitis(UC)is an inflammatory condition with frequent relapse and recurrence.Evidence suggests the involvement of SLC6A14 in UC pathogenesis,but the central regulator remains unknown.AIM To explore the role of SLC6A14 in UC-associated pyroptosis.METHODS Quantitative real-time polymerase chain reaction(qRT-PCR),immunoblotting,and immunohistochemical were used to assess SLC6A14 in human UC tissues.Lipopolysaccharide(LPS)was used to induce inflammation in FHC and NCM460 cells and model enteritis,and SLC6A14 levels were assessed.Pyroptosis markers were quantified using enzyme-linked immunosorbent assay,Western blotting,and qRT-PCR,and EdU incubation,CCK-8 assays and flow cytometry were used to examine proliferation and apoptosis.Mouse models of UC were used for verification.RESULTS SLC6A14 was increased and correlated with NLRP3 in UC tissues.LPS-induced FHC and NCM460 cells showed increased SLC6A14 levels.Reducing SLC6A14 increased cell proliferation and suppressed apoptosis.Reducing SLC6A14 decreased pyroptosis-associated proteins(ASC,IL-1β,IL-18,NLRP3).NLRP3 overexpression counteracted the effects of sh-SLC6A14 on LPS-induced FHC and NCM460 cell pyroptosis.SLC6A14 improved the mucosa in mice with dextran sulfate sodium-induced colitis.CONCLUSION SLC6A14 promotes UC pyroptosis by regulating NLRP3,suggesting the therapeutic potential of modulating the SLC6A14/NLRP3 axis.展开更多
Mitochondria are well cha racterized by their fundamental functions in regulating cellular homeostasis,including energy and iron metabolism.These functions are essential in neurons with high metabolic demands and elon...Mitochondria are well cha racterized by their fundamental functions in regulating cellular homeostasis,including energy and iron metabolism.These functions are essential in neurons with high metabolic demands and elongated neuronal processes.Mitochondria dynamically change morphology,localization,and activity to match neurons'spatial and temporal demands.Mitochondrial dysfunctions have been associated with many neurological disorders.展开更多
Objective： The aim of the study was to investigate apoptosis of the autonomic neuron in streptozotocin-induced diabetic rats, and observe the effect of intervention with nerve growth factor （NGF） on the apoptosis. ...Objective： The aim of the study was to investigate apoptosis of the autonomic neuron in streptozotocin-induced diabetic rats, and observe the effect of intervention with nerve growth factor （NGF） on the apoptosis. Methods： A total of 29 male Sprague-Dawley rats were divided into three groups, i.e. normal control （NC, n = 12）, untreated diabetic （DM, n = 9） and diabetic treated with NGF daily of 500 μg/kg for 30 days （DM＋NGF, n = 8）. The diabetic rat models were produced by intraperitoneal injection with streptozotocin （65 mg/kg ）. Over 3 months since the diabetes were setup, the superior cervical sympathetic ganglions（SCG） and the celiac ganglions（CG） were removed and fixed with 10% paraformaldchyde. Apoptosis was measured using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling（TUNEL）. Apoptotic index （AI） was calculated by computer image analysis system. Results： The AI of SCG and CG in DM and DM＋NGF group were significant higher than those of NC group （P 〈 0.001） respectively. There was no difference of AI of SCG and CG between DM group and DM＋NGF group （both P 〉 0.05）. Conclusion： Neuron apoptosis may contribute to the pathophysiology of diabetic autonomic neuropathy and NGF can not prevent the apoptosis of autonomic neuron in diabetic rats.展开更多
BACKGROUND After vaccination was mandated worldwide,various adverse effects associated with the coronavirus disease 2019(COVID-19)vaccination,including shoulder pain,have been reported.Here,we report a case of new-ons...BACKGROUND After vaccination was mandated worldwide,various adverse effects associated with the coronavirus disease 2019(COVID-19)vaccination,including shoulder pain,have been reported.Here,we report a case of new-onset shoulder pain after BNT162b2(Comirnaty,Pfizer-BioNTech)mRNA vaccination.CASE SUMMARY A 50-year-old man visited our rehabilitation center with left shoulder range of motion(ROM)limitation that had persisted for more than 5 mo.The history included no specific noteworthy events,except vaccination.The pain in the patient’s left deltoid muscle appeared 1 day after the second BNT162b2 vaccination and intensified to severe pain.The patient self-administered aspirin,with which the pain subsided immediately,whereas ROM limitation persisted.At the first visit,the patient complained of dull pain and ROM restriction of the left shoulder(flexion 130°,abduction 110°,and external rotation 40°).Among the diagnostic studies conducted for the evaluation of the shoulder,magnetic resonance imaging showed a thickened coracohumeral ligament.Nerve conduction studies and needle electromyography showed no electrodiagnostic abnormalities.The patient received comprehensive rehabilitation for 7 mo and had an overall improvement in pain and ROM of the left shoulder.CONCLUSION In this case of severe shoulder pain after COVID-19 vaccination that subsided immediately with aspirin treatment,the exact cause and mechanism of pain are unclear.However,the clinical symptoms and diagnostic workups in our report suggest the possibility that the COVID-19 vaccination triggered an immunochemical response that resulted in shoulder pathology.展开更多
Objective： To investigate the pathological changes following the focal cerebral ischemia and reperfusion in rats and the effect of acupuncture on them. Methods： A model of ischemia and reperfusion was established by...Objective： To investigate the pathological changes following the focal cerebral ischemia and reperfusion in rats and the effect of acupuncture on them. Methods： A model of ischemia and reperfusion was established by thread occlusion, and the brain activating acupuncture was applied, and then the morphological changes of the cerebral cortex on the ischemic side were observed under electron and light microscopes. Results： Cerebral ischemia and reperfusion could lead to the structural injury of neurons, gliacytes and capillary vessels in rat＇s brain, and acupuncture could improve the ultra-structural injury in the area around the cerebral ischemia. It was also found that acupuncture intervention at 3 hours following reperfusion was more ideal than that at any other time points. Conclusion： Acupuncture has a protective effect on the ultra-structural injury of neurons associated with focal cerebral ischemia and reperfusion in rats, and acupuncture given within 3 hours following reperfusion can produce a satisfactory effect.展开更多
基金Supported by the Shenyang Science and Technology,No.22-321-32-15Department of Science and Technology of Liaoning Province,No.2023JH2/101600015.
文摘BACKGROUND Gastric subepithelial lesions(SELs)are elevated lesions originating from the muscularis mucosa,submucosa,or muscularis propria,and may also include extraluminal lesions.For small SELs(less than 5 cm),complete endoscopic excision is the preferred treatment.Endoscopic full-thickness resection(EFTR)has proven to be an effective approach.AIM To evaluate the efficacy of the interrupted closure technique compared to the traditional closure technique in EFTR for gastric SELs.METHODS This single-center,prospective,randomized controlled trial was conducted at a tertiary hospital from September 2023 to September 2024.A total of 90 patients who underwent EFTR for gastric SELs were randomly allocated to either the interrupted closure group(n=44)or the traditional closure group(n=46).RESULTS All patients had complete resection and wound closure without any severe postoperative complications.The incidence of intraoperative gas-related complications was significantly lower in the interrupted closure group than in the traditional closure group(2.27%vs 26.09%,P=0.001),demonstrating interrupted closure technique can reduce the incidence of gas-related issues.Statistical analysis revealed that the incidence of postoperative infection was significantly lower in the experimental group than in the control group(15.91%vs 41.30%,P=0.008).Additionally,the median duration of antibiotic use was lower in the experimental group(3.5 days vs 5 days,P=0.013).Abdominal pain levels on postoperative days 1 and 4 were also lower in the experimental group compared to the control group(P<0.001).CONCLUSION The interrupted closure technique in EFTR for treating gastric SELs is safe and effective,reducing the incidence of intraoperative gas complications and postoperative infections.
基金Supported by The Key Research and Development Projects of Sichuan Science and Technology Department,No.2023YFS0285Natural Science Foundation Project of Sichuan Science and Technology Department,No.2023NSFSC0613.
文摘BACKGROUND Ulcerative colitis(UC)is an inflammatory condition with frequent relapse and recurrence.Evidence suggests the involvement of SLC6A14 in UC pathogenesis,but the central regulator remains unknown.AIM To explore the role of SLC6A14 in UC-associated pyroptosis.METHODS Quantitative real-time polymerase chain reaction(qRT-PCR),immunoblotting,and immunohistochemical were used to assess SLC6A14 in human UC tissues.Lipopolysaccharide(LPS)was used to induce inflammation in FHC and NCM460 cells and model enteritis,and SLC6A14 levels were assessed.Pyroptosis markers were quantified using enzyme-linked immunosorbent assay,Western blotting,and qRT-PCR,and EdU incubation,CCK-8 assays and flow cytometry were used to examine proliferation and apoptosis.Mouse models of UC were used for verification.RESULTS SLC6A14 was increased and correlated with NLRP3 in UC tissues.LPS-induced FHC and NCM460 cells showed increased SLC6A14 levels.Reducing SLC6A14 increased cell proliferation and suppressed apoptosis.Reducing SLC6A14 decreased pyroptosis-associated proteins(ASC,IL-1β,IL-18,NLRP3).NLRP3 overexpression counteracted the effects of sh-SLC6A14 on LPS-induced FHC and NCM460 cell pyroptosis.SLC6A14 improved the mucosa in mice with dextran sulfate sodium-induced colitis.CONCLUSION SLC6A14 promotes UC pyroptosis by regulating NLRP3,suggesting the therapeutic potential of modulating the SLC6A14/NLRP3 axis.
基金supported by grants from the National Institute on Aging(AG063766and AG028740 to RX,AG066654 to SMH,T32AG062728 to TM)the American Cancer Society(RSG-17-171-01-DMC to RX)the American Federation for Aging Research(AGR DT07-2502019 and AGR DTD 09-15-2021 to SMH)。
文摘Mitochondria are well cha racterized by their fundamental functions in regulating cellular homeostasis,including energy and iron metabolism.These functions are essential in neurons with high metabolic demands and elongated neuronal processes.Mitochondria dynamically change morphology,localization,and activity to match neurons'spatial and temporal demands.Mitochondrial dysfunctions have been associated with many neurological disorders.
文摘Objective： The aim of the study was to investigate apoptosis of the autonomic neuron in streptozotocin-induced diabetic rats, and observe the effect of intervention with nerve growth factor （NGF） on the apoptosis. Methods： A total of 29 male Sprague-Dawley rats were divided into three groups, i.e. normal control （NC, n = 12）, untreated diabetic （DM, n = 9） and diabetic treated with NGF daily of 500 μg/kg for 30 days （DM＋NGF, n = 8）. The diabetic rat models were produced by intraperitoneal injection with streptozotocin （65 mg/kg ）. Over 3 months since the diabetes were setup, the superior cervical sympathetic ganglions（SCG） and the celiac ganglions（CG） were removed and fixed with 10% paraformaldchyde. Apoptosis was measured using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling（TUNEL）. Apoptotic index （AI） was calculated by computer image analysis system. Results： The AI of SCG and CG in DM and DM＋NGF group were significant higher than those of NC group （P 〈 0.001） respectively. There was no difference of AI of SCG and CG between DM group and DM＋NGF group （both P 〉 0.05）. Conclusion： Neuron apoptosis may contribute to the pathophysiology of diabetic autonomic neuropathy and NGF can not prevent the apoptosis of autonomic neuron in diabetic rats.
文摘BACKGROUND After vaccination was mandated worldwide,various adverse effects associated with the coronavirus disease 2019(COVID-19)vaccination,including shoulder pain,have been reported.Here,we report a case of new-onset shoulder pain after BNT162b2(Comirnaty,Pfizer-BioNTech)mRNA vaccination.CASE SUMMARY A 50-year-old man visited our rehabilitation center with left shoulder range of motion(ROM)limitation that had persisted for more than 5 mo.The history included no specific noteworthy events,except vaccination.The pain in the patient’s left deltoid muscle appeared 1 day after the second BNT162b2 vaccination and intensified to severe pain.The patient self-administered aspirin,with which the pain subsided immediately,whereas ROM limitation persisted.At the first visit,the patient complained of dull pain and ROM restriction of the left shoulder(flexion 130°,abduction 110°,and external rotation 40°).Among the diagnostic studies conducted for the evaluation of the shoulder,magnetic resonance imaging showed a thickened coracohumeral ligament.Nerve conduction studies and needle electromyography showed no electrodiagnostic abnormalities.The patient received comprehensive rehabilitation for 7 mo and had an overall improvement in pain and ROM of the left shoulder.CONCLUSION In this case of severe shoulder pain after COVID-19 vaccination that subsided immediately with aspirin treatment,the exact cause and mechanism of pain are unclear.However,the clinical symptoms and diagnostic workups in our report suggest the possibility that the COVID-19 vaccination triggered an immunochemical response that resulted in shoulder pathology.
文摘Objective： To investigate the pathological changes following the focal cerebral ischemia and reperfusion in rats and the effect of acupuncture on them. Methods： A model of ischemia and reperfusion was established by thread occlusion, and the brain activating acupuncture was applied, and then the morphological changes of the cerebral cortex on the ischemic side were observed under electron and light microscopes. Results： Cerebral ischemia and reperfusion could lead to the structural injury of neurons, gliacytes and capillary vessels in rat＇s brain, and acupuncture could improve the ultra-structural injury in the area around the cerebral ischemia. It was also found that acupuncture intervention at 3 hours following reperfusion was more ideal than that at any other time points. Conclusion： Acupuncture has a protective effect on the ultra-structural injury of neurons associated with focal cerebral ischemia and reperfusion in rats, and acupuncture given within 3 hours following reperfusion can produce a satisfactory effect.
