Invasive-squamous-cell-cancer(ISCC)of the anal canal is an uncommon disease.Human papillomavirus(HPV)is the etiological agent of most of types of ISCC.The incidence of ISCC has been increasing in HIV-infected individu...Invasive-squamous-cell-cancer(ISCC)of the anal canal is an uncommon disease.Human papillomavirus(HPV)is the etiological agent of most of types of ISCC.The incidence of ISCC has been increasing in HIV-infected individuals,even after the introduction of highly active antiretroviral therapy.The aim of this study was to analyze biopsy specimens from patients diagnosed with ISCC at a tertiary hospital from 1983 to 2012 in order to detect HPV-DNA.Methods:Formaldehyde-fixed,paraffin-embedded specimens from patients with ISCC underwent HPV-DNA genotyping using multiplex PCR assay.Results:A total of 31 cases were collected;10 were HIV-infected(9 men,1 woman)and 21 non-HIV-infected(11 men,10 women).HPV infection was detected in 87.5%(7/8)of the HIV-infected patients(DNA from 2 biopsies was degraded)and 76.2%(16/21)of non-HIV-infected individuals.Multiple-type infections were only found in 28.6%(2/7)of the HIV-infected patients(no multiple-type infections in non-HIV-infected individuals).The most prevalent type was HPV-16:50%(4/8)in the HIV-infected group(57%[4/7]of the HPV-positive samples)and 66.7%(14/21)in the non-HIV-infected group(87.5%(14/16)of the HPV-positive samples).Remarkably,37.5%(3/8)of the HIV-infected group had high-risk HPV types not included in the vaccines(HPV-33,51,52,and 66)compared with 4.8%in the non-HIV-infected group(1/21,HPV-52).All cases of anal ISCC in HIV-infected patients were recorded in the highly active antiretroviral therapy era.Conclusion:HIV-infected patients presented anal ISCC with a higher proportion of high-risk HPV types not covered by the conventional vaccines than non-HIV-infected individuals.展开更多
基金from Red de Investigacion en SIDA(RIS)ART AIDS Foundation+2 种基金Gilead SciencesObra Social Caixa SabadellGala contra la SIDA Barcelona 2011
文摘Invasive-squamous-cell-cancer(ISCC)of the anal canal is an uncommon disease.Human papillomavirus(HPV)is the etiological agent of most of types of ISCC.The incidence of ISCC has been increasing in HIV-infected individuals,even after the introduction of highly active antiretroviral therapy.The aim of this study was to analyze biopsy specimens from patients diagnosed with ISCC at a tertiary hospital from 1983 to 2012 in order to detect HPV-DNA.Methods:Formaldehyde-fixed,paraffin-embedded specimens from patients with ISCC underwent HPV-DNA genotyping using multiplex PCR assay.Results:A total of 31 cases were collected;10 were HIV-infected(9 men,1 woman)and 21 non-HIV-infected(11 men,10 women).HPV infection was detected in 87.5%(7/8)of the HIV-infected patients(DNA from 2 biopsies was degraded)and 76.2%(16/21)of non-HIV-infected individuals.Multiple-type infections were only found in 28.6%(2/7)of the HIV-infected patients(no multiple-type infections in non-HIV-infected individuals).The most prevalent type was HPV-16:50%(4/8)in the HIV-infected group(57%[4/7]of the HPV-positive samples)and 66.7%(14/21)in the non-HIV-infected group(87.5%(14/16)of the HPV-positive samples).Remarkably,37.5%(3/8)of the HIV-infected group had high-risk HPV types not included in the vaccines(HPV-33,51,52,and 66)compared with 4.8%in the non-HIV-infected group(1/21,HPV-52).All cases of anal ISCC in HIV-infected patients were recorded in the highly active antiretroviral therapy era.Conclusion:HIV-infected patients presented anal ISCC with a higher proportion of high-risk HPV types not covered by the conventional vaccines than non-HIV-infected individuals.
