Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribun...Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribunda extracts were analyzed,including flavonoids and glucoside derivatives.Osteogenic activity was assessed in MC3T3-E1 preosteoblast cells by measurement of alkaline phosphatase(ALP)activity,alizarin red S staining,and the expression of osteogenic markers(RUNX2,SP7,and ALPL).In vivo effects were evaluated in zebrafish larvae by assessing skeletal development and expression of osteogenic genes(runx2a,sp7,and alpl).The role of mammalian target of rapamycin(mTOR)pathway was examined using rapamycin.Results:W.floribunda extracts significantly enhanced ALP activity,bone mineralization,and the expression of RUNX2,SP7,and ALPL in MC3T3-E1 cells.In zebrafish larvae,W.floribunda extracts improved vertebral mineralization and upregulated osteogenic genes.Mechanistically,the plant extract activated the mTOR pathway,and rapamycin treatment attenuated the extracts-induced ALP activity,mineralization,and vertebral formation in zebrafish,confirming mTOR involvement.Conclusions:W.floribunda extracts promote osteoblast differentiation and bone formation via mTOR pathway activation.These findings provide novel insights into the potential of W.floribunda extracts and support its further investigation as a natural therapeutic candidate for bone degenerative disorders such as osteoporosis.展开更多
基金supported by the 2024 scientific promotion program funded by Jeju National University.
文摘Objective:To investigate the osteogenic effects of polyphenol-rich extracts from Wisteria floribunda(Willd.)DC.(W.floribunda)flowers and elucidate the underlying mechanisms.Methods:Polyphenolic compounds of W.floribunda extracts were analyzed,including flavonoids and glucoside derivatives.Osteogenic activity was assessed in MC3T3-E1 preosteoblast cells by measurement of alkaline phosphatase(ALP)activity,alizarin red S staining,and the expression of osteogenic markers(RUNX2,SP7,and ALPL).In vivo effects were evaluated in zebrafish larvae by assessing skeletal development and expression of osteogenic genes(runx2a,sp7,and alpl).The role of mammalian target of rapamycin(mTOR)pathway was examined using rapamycin.Results:W.floribunda extracts significantly enhanced ALP activity,bone mineralization,and the expression of RUNX2,SP7,and ALPL in MC3T3-E1 cells.In zebrafish larvae,W.floribunda extracts improved vertebral mineralization and upregulated osteogenic genes.Mechanistically,the plant extract activated the mTOR pathway,and rapamycin treatment attenuated the extracts-induced ALP activity,mineralization,and vertebral formation in zebrafish,confirming mTOR involvement.Conclusions:W.floribunda extracts promote osteoblast differentiation and bone formation via mTOR pathway activation.These findings provide novel insights into the potential of W.floribunda extracts and support its further investigation as a natural therapeutic candidate for bone degenerative disorders such as osteoporosis.
