AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a grow...AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a growing number of tumor-derived cell lines, as well as tumor specimens from human cancers. The purpose of this study was to investigate the expression of p-Stat3, activated form of Stat3, and its downstream mediators including cyclin D1 and Bcl-XL in colorectal carcinoma (CRC), and to explore the possible mechanism of Stat3 signaling pathway in the tumorigenesis of colorectal carcinoma. METHODS: Tissue samples from 45 patients of primary colorectal carcinoma were selected for studying Stat3 signaling pathway protein expression. Western blot analysis was used to measure the expression of p-Stat3, cyclin D1, and Bcl-xu proteins in colorectal carcinomas. Furthermore, the expression patterns of these proteins were analyzed for their distribution at the cellular level by immunohistochemical staining of the tissues. RESULTS: Protein levels of p-Stat3, cyclin D1, and Bcl-XL were increased in colorectal carcinomas compared with adjacent normal mucosae (P<0.05). Elevated levels of pStat3 were correlated with the nodal metastasis and the stage (P<0.05). Overexpression of cyclin D1 was associated with the nodal metastasis (P<0.05). There was also a significant correlation between the expressions of p-Stat3 and cyclin D1 (r=0.382, P<0.05). CONCLUSION: Constitutive activation of Stat3 may play an important role in the tumorigenesis of colorectal carcinoma, and the detailed mechanism of Stat3 signaling pathway in CRC deserves further investigation.展开更多
We presented a 20-year-old patient with Crohn's disease (CD).Colonoscopy revealed longitudinal ulceration in the terminal ileum and rectal aphtoid ulcers.After treatment with mesalamine and total parenteral nutrit...We presented a 20-year-old patient with Crohn's disease (CD).Colonoscopy revealed longitudinal ulceration in the terminal ileum and rectal aphtoid ulcers.After treatment with mesalamine and total parenteral nutrition,repeat colonoscopy revealed a granular elevated area in the terminal ileum,which appeared as an irregular dome-like elevation with irregularly arranged villi on magnifying endoscopy.Biopsy specimens taken from the region showed microgranulomas and lymphoid hyperplasia,Scanning electron microscopy revealed the presence of M cells,confirming that the area corresponded to Peyer's patches.Peyer's patches by magnifying endoscopy and electron microscopy may provide insights into the pathogenesis of CD.展开更多
Background&Aims: The MDR1 gene encodes P-glycoprotein 170, an efflux transpo rter that is highly expressed in intestinal epithelial cells. The MDR1 exonic si ngle nucleotide polymorphisms(SNPs) C3435T and G2677T h...Background&Aims: The MDR1 gene encodes P-glycoprotein 170, an efflux transpo rter that is highly expressed in intestinal epithelial cells. The MDR1 exonic si ngle nucleotide polymorphisms(SNPs) C3435T and G2677T have been shown to correla te with activity/expression of P-glycoprotein 170.Methods: This was a case-con trol analysis of MDR1 C3435T and G2677T SNPs in a large well-characterized Scot tish white cohort (335 with ulcerative colitis [UC], 268 with Crohn’s disease[C D], and 370 healthy controls). We conducted 2-locus haplotype and detailed univ ariate and multivariate genotypicphenotypic analyses. Results: The MDR1 3435 TT genotype(34.6%vs 26.5%; P =. 04; odds ratio [OR], 1.60; 95%confidence interva l [95%CI], 1.04-2.44) and T-allelic frequencies(58.2%vs 52.8%; P =. 02; OR, 1.28; 95%CI, 1.03-1.58) were significantly higher in patients with UC compare d with controls.No associationwas seen with CD. The association was strongest wi th extensive UC (TT genotype: 42.4%vs 26.5%; P =. 003;OR, 2.64; 95%CI, 1.34- 4.99; and T allele: 63.9%vs 52.8%; P=. 009; OR, 1.70; 95%CI, 1.24-2.29), and this was also confirmed on multivariate analysis (P =. 007). The G2677T SNP was not associated with UC or CD. These 2 SNPs lie in linkage disequilibrium in our population (D′,. 8-.9; r2,. 7-.8). Two-locus haplotypes showed both positiv e (3435T/G2677 haplotype:P =. 03; OR, 1.44) and negative (C3435/2677T haplotype: P=. 002; OR,. 35) associations with UC. Homozygotes for the haplotype 3435T/G26 77 were significantly increased in UC (P=. 017; OR, 8.88; 95%CI, 1.10-71.45). Conclusions: Allelic variations of the MDR1 gene determine disease extent as wel l as susceptibility to UC in the Scottish population. The present data strongly implicate the C3435T SNP, although the 2-locus haplotype data underline the nee d for further detailed haplotypic studies.展开更多
基金Supported by the National Natural Science Foundation of China,No.30271269
文摘AIM: Signal transducers and activators of transcription (STATs) are a family of transcription factors activated in response to cytokines and growth factors. Constitutive activation of Stat3 has been observed in a growing number of tumor-derived cell lines, as well as tumor specimens from human cancers. The purpose of this study was to investigate the expression of p-Stat3, activated form of Stat3, and its downstream mediators including cyclin D1 and Bcl-XL in colorectal carcinoma (CRC), and to explore the possible mechanism of Stat3 signaling pathway in the tumorigenesis of colorectal carcinoma. METHODS: Tissue samples from 45 patients of primary colorectal carcinoma were selected for studying Stat3 signaling pathway protein expression. Western blot analysis was used to measure the expression of p-Stat3, cyclin D1, and Bcl-xu proteins in colorectal carcinomas. Furthermore, the expression patterns of these proteins were analyzed for their distribution at the cellular level by immunohistochemical staining of the tissues. RESULTS: Protein levels of p-Stat3, cyclin D1, and Bcl-XL were increased in colorectal carcinomas compared with adjacent normal mucosae (P<0.05). Elevated levels of pStat3 were correlated with the nodal metastasis and the stage (P<0.05). Overexpression of cyclin D1 was associated with the nodal metastasis (P<0.05). There was also a significant correlation between the expressions of p-Stat3 and cyclin D1 (r=0.382, P<0.05). CONCLUSION: Constitutive activation of Stat3 may play an important role in the tumorigenesis of colorectal carcinoma, and the detailed mechanism of Stat3 signaling pathway in CRC deserves further investigation.
文摘We presented a 20-year-old patient with Crohn's disease (CD).Colonoscopy revealed longitudinal ulceration in the terminal ileum and rectal aphtoid ulcers.After treatment with mesalamine and total parenteral nutrition,repeat colonoscopy revealed a granular elevated area in the terminal ileum,which appeared as an irregular dome-like elevation with irregularly arranged villi on magnifying endoscopy.Biopsy specimens taken from the region showed microgranulomas and lymphoid hyperplasia,Scanning electron microscopy revealed the presence of M cells,confirming that the area corresponded to Peyer's patches.Peyer's patches by magnifying endoscopy and electron microscopy may provide insights into the pathogenesis of CD.
文摘Background&Aims: The MDR1 gene encodes P-glycoprotein 170, an efflux transpo rter that is highly expressed in intestinal epithelial cells. The MDR1 exonic si ngle nucleotide polymorphisms(SNPs) C3435T and G2677T have been shown to correla te with activity/expression of P-glycoprotein 170.Methods: This was a case-con trol analysis of MDR1 C3435T and G2677T SNPs in a large well-characterized Scot tish white cohort (335 with ulcerative colitis [UC], 268 with Crohn’s disease[C D], and 370 healthy controls). We conducted 2-locus haplotype and detailed univ ariate and multivariate genotypicphenotypic analyses. Results: The MDR1 3435 TT genotype(34.6%vs 26.5%; P =. 04; odds ratio [OR], 1.60; 95%confidence interva l [95%CI], 1.04-2.44) and T-allelic frequencies(58.2%vs 52.8%; P =. 02; OR, 1.28; 95%CI, 1.03-1.58) were significantly higher in patients with UC compare d with controls.No associationwas seen with CD. The association was strongest wi th extensive UC (TT genotype: 42.4%vs 26.5%; P =. 003;OR, 2.64; 95%CI, 1.34- 4.99; and T allele: 63.9%vs 52.8%; P=. 009; OR, 1.70; 95%CI, 1.24-2.29), and this was also confirmed on multivariate analysis (P =. 007). The G2677T SNP was not associated with UC or CD. These 2 SNPs lie in linkage disequilibrium in our population (D′,. 8-.9; r2,. 7-.8). Two-locus haplotypes showed both positiv e (3435T/G2677 haplotype:P =. 03; OR, 1.44) and negative (C3435/2677T haplotype: P=. 002; OR,. 35) associations with UC. Homozygotes for the haplotype 3435T/G26 77 were significantly increased in UC (P=. 017; OR, 8.88; 95%CI, 1.10-71.45). Conclusions: Allelic variations of the MDR1 gene determine disease extent as wel l as susceptibility to UC in the Scottish population. The present data strongly implicate the C3435T SNP, although the 2-locus haplotype data underline the nee d for further detailed haplotypic studies.
