The intestinal epithelium undergoes rapid renewal,with the entire epithelial layer replaced within five days.Intestinal stem cells(ISCs),located in the intestinal crypts,generate all differentiated cell types necessar...The intestinal epithelium undergoes rapid renewal,with the entire epithelial layer replaced within five days.Intestinal stem cells(ISCs),located in the intestinal crypts,generate all differentiated cell types necessary for intestinal function.Key signalling pathways involved in stem cell maintenance include Wnt,Notch,Hedgehog,and BMP.Wnt signalling,primarily driven by crypt cells,creates a signalling gradient to maintain homeostasis[1].However,nuclearβ-catenin,the key regulator of Wnt signalling,correlates positively with tumorigenesis.展开更多
Semantic segmentation plays a foundational role in biomedical image analysis, providing precise information about cellular, tissue, and organ structures in both biological and medical imaging modalities. Traditional a...Semantic segmentation plays a foundational role in biomedical image analysis, providing precise information about cellular, tissue, and organ structures in both biological and medical imaging modalities. Traditional approaches often fail in the face of challenges such as low contrast, morphological variability, and densely packed structures. Recent advancements in deep learning have transformed segmentation capabilities through the integration of fine-scale detail preservation, coarse-scale contextual modeling, and multi-scale feature fusion. This work provides a comprehensive analysis of state-of-the-art deep learning models, including U-Net variants, attention-based frameworks, and Transformer-integrated networks, highlighting innovations that improve accuracy, generalizability, and computational efficiency. Key architectural components such as convolution operations, shallow and deep blocks, skip connections, and hybrid encoders are examined for their roles in enhancing spatial representation and semantic consistency. We further discuss the importance of hierarchical and instance-aware segmentation and annotation in interpreting complex biological scenes and multiplexed medical images. By bridging methodological developments with diverse application domains, this paper outlines current trends and future directions for semantic segmentation, emphasizing its critical role in facilitating annotation, diagnosis, and discovery in biomedical research.展开更多
Phase change microcapsules can carry large amounts of heat and be dispersed into other mediums either as a solid composite or as slurry fluids without changes to their appearance or fluidity. These two standout featur...Phase change microcapsules can carry large amounts of heat and be dispersed into other mediums either as a solid composite or as slurry fluids without changes to their appearance or fluidity. These two standout features make phase change microcapsules ideal for use in thermal energy applications to enhance the efficiency of energy utilisation. This review paper includes methods used for the encapsulation of phase change materials, especially the method suitable for large scale productions, the trends of phase change microcapsule development and their use in thermal energy applications in static and dynamic conditions. The effect of phase change microcapsules on convective heat transfer through addition to thermal fluids as slurries is critically reviewed. The review highlighted that so far the phase change microcapsules used mainly have polymeric shells, which has very low thermal conductivities. Their enhancement in convective heat transfer was demonstrated in locations where the phase change material experiences phase change. The phase change results in the slurries having higher apparent local specific heat capacities and thus higher local heat transfer coefficients. Out of the phase change region, no enhancement is observed from the solid microcapsule particles due to the low specific heat capacity and thermal conductivity of the phase change microcapsules compared to that of water, which is normally used as slurry media in the test. To further the research in this area, phase change microcapsules with higher specific heat capacity, higher thermal conductivity and better shape stability need to be applied.展开更多
基金supported by the Medical Research Council(MRC)(grant number G0700763)by the University of Nottingham,Nottingham,UK.
文摘The intestinal epithelium undergoes rapid renewal,with the entire epithelial layer replaced within five days.Intestinal stem cells(ISCs),located in the intestinal crypts,generate all differentiated cell types necessary for intestinal function.Key signalling pathways involved in stem cell maintenance include Wnt,Notch,Hedgehog,and BMP.Wnt signalling,primarily driven by crypt cells,creates a signalling gradient to maintain homeostasis[1].However,nuclearβ-catenin,the key regulator of Wnt signalling,correlates positively with tumorigenesis.
基金Open Access funding provided by the National Institutes of Health(NIH)The funding for this project was provided by NCATS Intramural Fund.
文摘Semantic segmentation plays a foundational role in biomedical image analysis, providing precise information about cellular, tissue, and organ structures in both biological and medical imaging modalities. Traditional approaches often fail in the face of challenges such as low contrast, morphological variability, and densely packed structures. Recent advancements in deep learning have transformed segmentation capabilities through the integration of fine-scale detail preservation, coarse-scale contextual modeling, and multi-scale feature fusion. This work provides a comprehensive analysis of state-of-the-art deep learning models, including U-Net variants, attention-based frameworks, and Transformer-integrated networks, highlighting innovations that improve accuracy, generalizability, and computational efficiency. Key architectural components such as convolution operations, shallow and deep blocks, skip connections, and hybrid encoders are examined for their roles in enhancing spatial representation and semantic consistency. We further discuss the importance of hierarchical and instance-aware segmentation and annotation in interpreting complex biological scenes and multiplexed medical images. By bridging methodological developments with diverse application domains, this paper outlines current trends and future directions for semantic segmentation, emphasizing its critical role in facilitating annotation, diagnosis, and discovery in biomedical research.
文摘Phase change microcapsules can carry large amounts of heat and be dispersed into other mediums either as a solid composite or as slurry fluids without changes to their appearance or fluidity. These two standout features make phase change microcapsules ideal for use in thermal energy applications to enhance the efficiency of energy utilisation. This review paper includes methods used for the encapsulation of phase change materials, especially the method suitable for large scale productions, the trends of phase change microcapsule development and their use in thermal energy applications in static and dynamic conditions. The effect of phase change microcapsules on convective heat transfer through addition to thermal fluids as slurries is critically reviewed. The review highlighted that so far the phase change microcapsules used mainly have polymeric shells, which has very low thermal conductivities. Their enhancement in convective heat transfer was demonstrated in locations where the phase change material experiences phase change. The phase change results in the slurries having higher apparent local specific heat capacities and thus higher local heat transfer coefficients. Out of the phase change region, no enhancement is observed from the solid microcapsule particles due to the low specific heat capacity and thermal conductivity of the phase change microcapsules compared to that of water, which is normally used as slurry media in the test. To further the research in this area, phase change microcapsules with higher specific heat capacity, higher thermal conductivity and better shape stability need to be applied.
