Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferatio...Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferation of vascular and proliferation of cultured smooth muscle cells(vascular smooth muscle cells, VSMCs)were observed by the means of computerised image analysis and MTT method respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. Results: One week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they was more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control).TEA(blocker of Ca2+ activated potassium channels)increased MTT A490 nm value of VSMCs from femoral vein in a dose dependent manner(P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts (1-4) week after transplantation(P<0.05, n=5).Conclusion: KCa is inhibited in autogenous vein graft, which account for vasospasm and intimal proliferation.展开更多
We present a case of stent graft collapse after performing thoracic endovascular aortic repair with a custom-made fenestrated stent graft. The patient was a 70-year-old woman with an asymptomatic aneurysm of the dista...We present a case of stent graft collapse after performing thoracic endovascular aortic repair with a custom-made fenestrated stent graft. The patient was a 70-year-old woman with an asymptomatic aneurysm of the distal aortic arch, and thoracic endovascular aortic repair was performed. The patient showed a blood pressure difference between the left arm and the right arm on postoperative day (POD) 17 prompting the performance of a chest computed tomography scan which revealed stent graft collapse. She then underwent staged debranching of thoracic endovascular aortic repair. Stent graft collapse is a rare but well-described complication of thoracic endovascular repair. Therefore, patients who undergo such a procedure should be carefully monitored for signs and symptoms, which suggest the possibility of stent collapse.展开更多
Objective: To study the cause and mechanism of transplantation vasculopathy which characterized by accelerated graft arteriosclerosis (AGA), we established a mouse aorta graft model. Methods: A segment of thoracic aor...Objective: To study the cause and mechanism of transplantation vasculopathy which characterized by accelerated graft arteriosclerosis (AGA), we established a mouse aorta graft model. Methods: A segment of thoracic aortas of B10.A (2R) mice were transplanted to C57BL/10 mice abdominal aorta by end to side anastomoses. The different time point collected grafts were analyzed by morphological, histochemical and electro microscopic methods. Results: Rejection was manifested as a concentric progressive destruction of the smooth muscle cells. In contrast, the endothelial inflammation and subsequent neointimal proliferation characteristic of AGA was localized to the regions of turbulent flow, i.e. the junction of the graft with the recipient aorta. Conclusion: This model separates the processes of rejection and neointimal formation which usually manifested together in the lesion of AGA, elucidate that different mechanisms control vascular rejection and neointimal formation in chronic rejection.展开更多
Ⅰ. INTRODUCTION Recently, grafted polymers used as a non-thrombogenic material have been prepared by graft copolymerization of hydrophilic monomers, such as acrylamide (AAM) or 2-hydroxyethyl methacrylate onto the ba...Ⅰ. INTRODUCTION Recently, grafted polymers used as a non-thrombogenic material have been prepared by graft copolymerization of hydrophilic monomers, such as acrylamide (AAM) or 2-hydroxyethyl methacrylate onto the backbone of polyether urethane or polyvinyl alcohol, in which some ceric salt has been extensively used as an effective initiator in the heterogeneous phase graft copolymerization at room temperature.展开更多
We have studied the polymerization of vinyl monomers such as acrylamide (AAM)and methyl methacrylate (MMA) initiated with tetrahydrofuran-2-hydroperoxide (THFHP) and N, N-dimethyl-p-toluidine (DMT) redox system at 35...We have studied the polymerization of vinyl monomers such as acrylamide (AAM)and methyl methacrylate (MMA) initiated with tetrahydrofuran-2-hydroperoxide (THFHP) and N, N-dimethyl-p-toluidine (DMT) redox system at 35℃—55℃. In this paper, we wish to report a new method of graft copolymerization of展开更多
After organ transplantation,rapid repair of injured vascular endothelial cell(VEC)is a key to prevent graft chronic dysfunction besides control of immunological rejection.Many studies have confirmed that vascular endo...After organ transplantation,rapid repair of injured vascular endothelial cell(VEC)is a key to prevent graft chronic dysfunction besides control of immunological rejection.Many studies have confirmed that vascular endothelial growth factor 165(VEGF165)could accelerate the repair of VEC injury,decrease thrombosis and thrombotic occlusion,and inhibit hyperplasia of the intima.This study was designed to construct eukaryotic expression plasmid pBudCE4.1/VEGF165,and observe its effect on the prolife ration of VEC.METHODS:The VEGF165 gene cloned from human heart tissue by RT-PCR was cloned into eukaryotic expression plasmid pBudCE4.1.The recombinant expression plasmid pBudCE4.1/VEGF165 was identified by restriction enzyme(Hind III and BamH I)digestion analysis,and was sequenced.The pBudCE4.1/VEGF165 was introduced into VEC through lipofection transfection.The VEGF165 mRNA expression by Northern blot and VEGF165 protein expression was detected by immunocytochemical staining.The effect of expression protein on VEC proliferation was detected by flow cytometry.RESULTS:The RT-PCR product of the VEGF165 gene was about 576bp.Sequencing analysis revealed that the sequence of the amplified VEGF165 gene was identical with that in GenBank.Restrictive enzyme digestion analysis showed that recombinant expression plasmid pBudCE4.1/tVEGF165 had been constructed successfully.The expression of VEGF165 at mRNA and protein levels in the transformed VSMCs had been demonstrated by Northern blot and immunocytochemical staining respectively.The expressed product of VEGF165 could notably accelerate the proliferation of VECs.CONCLUSIONS:pBudCE4.1/VEGF165 is successfully cons-tructed and is expressed in VECs.Expressed VEGF165 can accelerate the VEC proliferation.The present study has laid a foundation for potential use of VEGF165 gene transfection to prevent and treat vascular stenosis in the transplanted organ.展开更多
AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model...AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model and we evaluated HIF-1αin steatotic and non-steatotic livers preserved for 24 h at 4℃in University of Wisconsin and IGL-1 solutions,and then subjected to 2 h of normothermic reperfusion.After normoxic reperfusion,liver enzymes,bile production,bromosulfophthalein clearance,as well as HIF-1αand NO[endothelial NO synthase(eNOS)activity and nitrites/nitrates]were also measured.Other factors associated with the higher susceptibility of steatotic livers to IRI,such as mitochondrial damage and vascular resistance were evaluated. RESULTS:A significant increase in HIF-1αwas found in steatotic and non-steatotic livers preserved in IGL-1 after cold storage.Livers preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function parameters.These benefits were enhanced by the addition of trimetazidine(an antiischemic drug),which induces NO and eNOS activation, to IGL-1 solution.In normoxic reperfusion,the presence of NO favors HIF-1αaccumulation,promoting also the activation of other cytoprotective genes,such as hemeoxygenase-1. CONCLUSION:We found evidence for the role of the HIF-1α/NO system in fatty liver preservation,especially when IGL-1 solution is used.展开更多
Recent studies have shown that fibroblast transplantation can modify the activity of basal ganglia networks in models of Parkinson＇s disease. To determine its effects on parkinsonian motor symptoms, we performed auto...Recent studies have shown that fibroblast transplantation can modify the activity of basal ganglia networks in models of Parkinson＇s disease. To determine its effects on parkinsonian motor symptoms, we performed autologous dermal fibroblast transplantation into the internal pallidum （GPi） in two parkinsonian rhesus monkeys with stable levodopa- induced dyskinesias （LIDs）. Levodopa responses were assessed every week after transplantation for three months. A reduction of between 58% and 64% in total LIDs on the contralateral side was observed in both animals. No clear LID changes were observed on the ipsilateral side. These effects lasted the entire 3-month period in one monkey, but declined after 6-8 weeks in the other. The antiparkinsonian effects of levodopa did not diminish, The results of this pilot study indicate that fibroblast transplantation into the GPi may have beneficial effects on LIDs and warrant further investigation for potential therapeutic use.展开更多
Objective: To establish a stable reduced-size hepatic transplantation model in rats. Methods: Liver transplantation was performed in accordance with Kamada techniques. Many modifications were made including: Surgical ...Objective: To establish a stable reduced-size hepatic transplantation model in rats. Methods: Liver transplantation was performed in accordance with Kamada techniques. Many modifications were made including: Surgical manipulative innovation, adjustment of pre-operative drug administration and removed liver volume. Results: Forty-two rats underwent reduced-size hepatic transplantation; of them 33(84.6%) survived more than 1 week. The causes of postoperative death were peritonitis, inferior vena cava thrombus and unknown complications. Conclusion: Manipulative innovation and proper drug administration can improve the survival rate of rats apparently. Grafts regeneration can be triggered by the removal of left lateral segment and caudate lobe.展开更多
Autoimmunity is believed to develop when genetically predisposed individuals undergo epigenetic modifcations in response to environmental factors. Recent advances in the understanding of epigenetic mechanisms suggest,...Autoimmunity is believed to develop when genetically predisposed individuals undergo epigenetic modifcations in response to environmental factors. Recent advances in the understanding of epigenetic mechanisms suggest, in autoimmune diseases, a multi-step process involving environmental factors （e.g. , drugs, stress） and endogenous factors （e.g. , cytokines, gender）, both leading to the deregulation of the epigenetic machinery （DNA methylation, histone modifications, miRNA）, that in turn specifically affects the immune system and/or the target organ（s）. Such effect is reinforced in those patients with risk variants mapping to epigenetically-controlled regulators of immune cells. As a consequence, autoreactive lymphocytes and autoantibodies are produced leading to the development of the autoimmune disease. Potential new therapeutic strategies and biomarkers are also addressed.展开更多
文摘Objective: To investigate changes of Ca2+ activated potassium channels (KCa) in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferation of vascular and proliferation of cultured smooth muscle cells(vascular smooth muscle cells, VSMCs)were observed by the means of computerised image analysis and MTT method respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. Results: One week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P<0.05, n=8 vs control), and they was more enhanced 4 weeks after vein transplantation (P<0.01, n=8 vs control).TEA(blocker of Ca2+ activated potassium channels)increased MTT A490 nm value of VSMCs from femoral vein in a dose dependent manner(P<0.05, n=8). KCa current density was significantly attenuated in VSMCs from autogenous vein grafts (1-4) week after transplantation(P<0.05, n=5).Conclusion: KCa is inhibited in autogenous vein graft, which account for vasospasm and intimal proliferation.
文摘We present a case of stent graft collapse after performing thoracic endovascular aortic repair with a custom-made fenestrated stent graft. The patient was a 70-year-old woman with an asymptomatic aneurysm of the distal aortic arch, and thoracic endovascular aortic repair was performed. The patient showed a blood pressure difference between the left arm and the right arm on postoperative day (POD) 17 prompting the performance of a chest computed tomography scan which revealed stent graft collapse. She then underwent staged debranching of thoracic endovascular aortic repair. Stent graft collapse is a rare but well-described complication of thoracic endovascular repair. Therefore, patients who undergo such a procedure should be carefully monitored for signs and symptoms, which suggest the possibility of stent collapse.
文摘Objective: To study the cause and mechanism of transplantation vasculopathy which characterized by accelerated graft arteriosclerosis (AGA), we established a mouse aorta graft model. Methods: A segment of thoracic aortas of B10.A (2R) mice were transplanted to C57BL/10 mice abdominal aorta by end to side anastomoses. The different time point collected grafts were analyzed by morphological, histochemical and electro microscopic methods. Results: Rejection was manifested as a concentric progressive destruction of the smooth muscle cells. In contrast, the endothelial inflammation and subsequent neointimal proliferation characteristic of AGA was localized to the regions of turbulent flow, i.e. the junction of the graft with the recipient aorta. Conclusion: This model separates the processes of rejection and neointimal formation which usually manifested together in the lesion of AGA, elucidate that different mechanisms control vascular rejection and neointimal formation in chronic rejection.
文摘Ⅰ. INTRODUCTION Recently, grafted polymers used as a non-thrombogenic material have been prepared by graft copolymerization of hydrophilic monomers, such as acrylamide (AAM) or 2-hydroxyethyl methacrylate onto the backbone of polyether urethane or polyvinyl alcohol, in which some ceric salt has been extensively used as an effective initiator in the heterogeneous phase graft copolymerization at room temperature.
文摘We have studied the polymerization of vinyl monomers such as acrylamide (AAM)and methyl methacrylate (MMA) initiated with tetrahydrofuran-2-hydroperoxide (THFHP) and N, N-dimethyl-p-toluidine (DMT) redox system at 35℃—55℃. In this paper, we wish to report a new method of graft copolymerization of
文摘After organ transplantation,rapid repair of injured vascular endothelial cell(VEC)is a key to prevent graft chronic dysfunction besides control of immunological rejection.Many studies have confirmed that vascular endothelial growth factor 165(VEGF165)could accelerate the repair of VEC injury,decrease thrombosis and thrombotic occlusion,and inhibit hyperplasia of the intima.This study was designed to construct eukaryotic expression plasmid pBudCE4.1/VEGF165,and observe its effect on the prolife ration of VEC.METHODS:The VEGF165 gene cloned from human heart tissue by RT-PCR was cloned into eukaryotic expression plasmid pBudCE4.1.The recombinant expression plasmid pBudCE4.1/VEGF165 was identified by restriction enzyme(Hind III and BamH I)digestion analysis,and was sequenced.The pBudCE4.1/VEGF165 was introduced into VEC through lipofection transfection.The VEGF165 mRNA expression by Northern blot and VEGF165 protein expression was detected by immunocytochemical staining.The effect of expression protein on VEC proliferation was detected by flow cytometry.RESULTS:The RT-PCR product of the VEGF165 gene was about 576bp.Sequencing analysis revealed that the sequence of the amplified VEGF165 gene was identical with that in GenBank.Restrictive enzyme digestion analysis showed that recombinant expression plasmid pBudCE4.1/tVEGF165 had been constructed successfully.The expression of VEGF165 at mRNA and protein levels in the transformed VSMCs had been demonstrated by Northern blot and immunocytochemical staining respectively.The expressed product of VEGF165 could notably accelerate the proliferation of VECs.CONCLUSIONS:pBudCE4.1/VEGF165 is successfully cons-tructed and is expressed in VECs.Expressed VEGF165 can accelerate the VEC proliferation.The present study has laid a foundation for potential use of VEGF165 gene transfection to prevent and treat vascular stenosis in the transplanted organ.
基金Supported by The Ministerio de de Sanidad y Consumo(PI081988)CIBER-EHD,Instituto Carlos Ⅲ,Madrid and Ministerio de Asuntos Exteriores y de Cooperación Internacionales(A/020255/08 and A/02987/09),Madrid
文摘AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model and we evaluated HIF-1αin steatotic and non-steatotic livers preserved for 24 h at 4℃in University of Wisconsin and IGL-1 solutions,and then subjected to 2 h of normothermic reperfusion.After normoxic reperfusion,liver enzymes,bile production,bromosulfophthalein clearance,as well as HIF-1αand NO[endothelial NO synthase(eNOS)activity and nitrites/nitrates]were also measured.Other factors associated with the higher susceptibility of steatotic livers to IRI,such as mitochondrial damage and vascular resistance were evaluated. RESULTS:A significant increase in HIF-1αwas found in steatotic and non-steatotic livers preserved in IGL-1 after cold storage.Livers preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function parameters.These benefits were enhanced by the addition of trimetazidine(an antiischemic drug),which induces NO and eNOS activation, to IGL-1 solution.In normoxic reperfusion,the presence of NO favors HIF-1αaccumulation,promoting also the activation of other cytoprotective genes,such as hemeoxygenase-1. CONCLUSION:We found evidence for the role of the HIF-1α/NO system in fatty liver preservation,especially when IGL-1 solution is used.
基金supported by grants from the National Institutes of Health (NS045962, NS073994, NCRR RR000165 and ORIP/OD OD011132)Forum Pharmaceuticals, Inc.Gene Graft, Ltd
文摘Recent studies have shown that fibroblast transplantation can modify the activity of basal ganglia networks in models of Parkinson＇s disease. To determine its effects on parkinsonian motor symptoms, we performed autologous dermal fibroblast transplantation into the internal pallidum （GPi） in two parkinsonian rhesus monkeys with stable levodopa- induced dyskinesias （LIDs）. Levodopa responses were assessed every week after transplantation for three months. A reduction of between 58% and 64% in total LIDs on the contralateral side was observed in both animals. No clear LID changes were observed on the ipsilateral side. These effects lasted the entire 3-month period in one monkey, but declined after 6-8 weeks in the other. The antiparkinsonian effects of levodopa did not diminish, The results of this pilot study indicate that fibroblast transplantation into the GPi may have beneficial effects on LIDs and warrant further investigation for potential therapeutic use.
文摘Objective: To establish a stable reduced-size hepatic transplantation model in rats. Methods: Liver transplantation was performed in accordance with Kamada techniques. Many modifications were made including: Surgical manipulative innovation, adjustment of pre-operative drug administration and removed liver volume. Results: Forty-two rats underwent reduced-size hepatic transplantation; of them 33(84.6%) survived more than 1 week. The causes of postoperative death were peritonitis, inferior vena cava thrombus and unknown complications. Conclusion: Manipulative innovation and proper drug administration can improve the survival rate of rats apparently. Grafts regeneration can be triggered by the removal of left lateral segment and caudate lobe.
基金Supported by The"Région Bretagne",the"Association Franaise Gougerot-Sjgren et des Syndromes Secs"the"Institut Franais pour la Recherche Odontologique"+1 种基金the Innovative Medicines Initiative Joint Undertaking under grant agreement n°115565,resources of which are composed of financial contribution from the European Union’s Seventh Framework Programme(FP7/2007-2013)EFPIA companies’ in-kind contribution
文摘Autoimmunity is believed to develop when genetically predisposed individuals undergo epigenetic modifcations in response to environmental factors. Recent advances in the understanding of epigenetic mechanisms suggest, in autoimmune diseases, a multi-step process involving environmental factors （e.g. , drugs, stress） and endogenous factors （e.g. , cytokines, gender）, both leading to the deregulation of the epigenetic machinery （DNA methylation, histone modifications, miRNA）, that in turn specifically affects the immune system and/or the target organ（s）. Such effect is reinforced in those patients with risk variants mapping to epigenetically-controlled regulators of immune cells. As a consequence, autoreactive lymphocytes and autoantibodies are produced leading to the development of the autoimmune disease. Potential new therapeutic strategies and biomarkers are also addressed.
