Employing the orthogonal design,the optimal extraction conditions for flavonoid in leaves of black currant were determined by colorimetric estimation,which were extraction agent of 75% EtOH-H 2O,the material ratio(ra...Employing the orthogonal design,the optimal extraction conditions for flavonoid in leaves of black currant were determined by colorimetric estimation,which were extraction agent of 75% EtOH-H 2O,the material ratio(ratio of weight of fresh leaves to volume of the solvent)of 1:16,the temperature of 35℃,the time of 1 h and times of three.A total extraction rate of over 97% and the flavonoid contents of 179 33 mg/100 g were obtained under the optimal extraction conditions.展开更多
文摘Employing the orthogonal design,the optimal extraction conditions for flavonoid in leaves of black currant were determined by colorimetric estimation,which were extraction agent of 75% EtOH-H 2O,the material ratio(ratio of weight of fresh leaves to volume of the solvent)of 1:16,the temperature of 35℃,the time of 1 h and times of three.A total extraction rate of over 97% and the flavonoid contents of 179 33 mg/100 g were obtained under the optimal extraction conditions.
