Red bayberry(Morella rubra)is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published.We updated the linkage map of the species by adding 118 SSR markers and the females...Red bayberry(Morella rubra)is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published.We updated the linkage map of the species by adding 118 SSR markers and the femalespecific marker MrFT2_BD-SEX.The integrated map included eight linkage groups and spanned 491 cM.Eleven sexassociated markers were identified,six of which were located in linkage group 8,in agreement with the previously reported location of the sex-determining region.The MrFT2_BD-SEX marker was genotyped in 203 cultivated accessions.Among the females of the accessions,we found two female-specific alleles,designated W-b(151 bp)and W-d(129 bp).We previously found that‘Dongkui’,a female cultivar,could produce viable pollen(we refer to such plants‘Dongkui-male’)and serve as the paternal parent in crosses.The genotypes of the MrFT2_BD-SEX marker were W-b/Z in‘Biqi’and W-d/Z in‘Dongkui-male’.The progeny of a cross between these parents produced a 3:1 female(W-)to male(ZZ)ratio and the expected 1:1:1:1 ratio of W-b/W-d:W-b/Z:W-d/Z:Z/Z.In addition,the flowering and fruiting phenotypes of all the F1 progeny fit their genotypes.Our results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus(W)in a small genomic region(59 kb and less than 3.3 cM).Furthermore,we have produced a homozygous“super female”(WW)that should produce all-female offspring in the F2 generation,providing a foundation for commercial use and presenting great potential for use in modern breeding programs.展开更多
A series of chemotherapeutic drugs that induce DNA damage,such as cisplatin(DDP),are standard clinical treatments for ovarian cancer,testicular cancer,and other diseases that lack effective targeted drug therapy.Drug ...A series of chemotherapeutic drugs that induce DNA damage,such as cisplatin(DDP),are standard clinical treatments for ovarian cancer,testicular cancer,and other diseases that lack effective targeted drug therapy.Drug resistance is one of the main factors limiting their application.Sensitizers can overcome the drug resistance of tumor cells,thereby enhancing the antitumor activity of chemotherapeutic drugs.In this study,we aimed to identify marketable drugs that could be potential chemotherapy sensitizers and explore the underlying mechanisms.We found that the alcohol withdrawal drug disulfiram(DSF)could significantly enhance the antitumor activity of DDP.JC-1 staining,propidium iodide(PI)staining,and western blotting confirmed that the combination of DSF and DDP could enhance the apoptosis of tumor cells.Subsequent RNA sequencing combined with Gene Set Enrichment Analysis(GSEA)pathway enrichment analysis and cell biology studies such as immunofluorescence suggested an underlying mechanism:DSF makes cells more vulnerable to DNA damage by inhibiting the Fanconi anemia(FA)repair pathway,exerting a sensitizing effect to DNA damaging agents including platinum chemotherapy drugs.Thus,our study illustrated the potential mechanism of action of DSF in enhancing the antitumor effect of DDP.This might provide an effective and safe solution for combating DDP resistance in clinical treatment.展开更多
Oral ulcers,a common mucosal affliction,severely diminish patients’quality of life due to pain and discomfort.Despite existing treatments,the frequent recurrence of oral ulcers underscores the need for more effective...Oral ulcers,a common mucosal affliction,severely diminish patients’quality of life due to pain and discomfort.Despite existing treatments,the frequent recurrence of oral ulcers underscores the need for more effective therapeutic options.This study aimed to investigate the inhibitory effects of Ponkan(Citrus reticulata‘Ponkan’)extracts and their flavonoid components on oral ulcers,exploring their potential as a natural therapy.Twenty different Ponkan cultivars were selected for flavonoid extraction and analysis.Ultra-performance liquid chromatography(UPLC)and high-resolution mass spectrometry(HRMS)were utilized for substance identification,leading to the discovery of 16 flavonoid compounds.Using an oral ulcer model established in Wistar rats,Ponkan extracts were found to effectively reduce ulcer area and pain sensitivity.The study revealed that four polymethoxyflavones(PMFs)were particularly effective in treating oral ulcers,while hesperidin did not show significant improvement.Mechanistic studies indicated that PMFs could inhibit the phosphorylation of Jak2 and Stat3,whereas hesperidin lacked this effect.This research supports the potential use of Ponkan and its flavonoids as a natural and effective alternative therapy for oral ulcers,with a focus on the role of PMFs in modulating the Jak2-Stat3 pathway,highlighting the possibilities for targeted natural treatments.展开更多
To enhance the utilization of pesticides and reduce environmental risks,we constructed the magnetic recyclable and dual stimulus-responsive microspheres to achieve on-demand pesticide release.Magnetic multi-shell holl...To enhance the utilization of pesticides and reduce environmental risks,we constructed the magnetic recyclable and dual stimulus-responsive microspheres to achieve on-demand pesticide release.Magnetic multi-shell hollow mesoporous organosilicon nanoparticles(mMSN)were prepared by one-step hydrothermal method and loaded with pesticide avermectin(A@mMSN),afterward A@mMSN was coated with gelatin through emulsification and chemical cross-linking to prepare A@mMSN@G microspheres(21.5±9.7μm).After being absorbed by the pests,the gelatin layer was hydrolyzed with the neutral protease,and the disulfide bonds within mMSN framework were decomposed by glutathione(GSH),endowing A@mMSN@G microspheres with enzyme and GSH responsiveness to achieve sustained avermectin release till 7 days(about 3.5 times that of the commercial avermectin emulsion).Importantly,the A@mMSN@G microspheres containing Fe3O4 nanoparticles could be easily magnetically collected from soil with a recovery ratio of 63.7%,to reduce the environmental risks.With excellent biosafety,A@mMSN@G microspheres showed outstanding pest control effects till two weeks and the growth of cabbage was not affected by it.Therefore,based on the recyclability and dual stimulus-responsive controllable release,the fabricated A@mMSN@G microspheres have broad application potential in pesticide delivery.展开更多
基金support from the Natural Science Foundation of China(31972364)Yuyao Forestry Technology Extension Center.H.M.J.was supported by the National Natural Science Foundation of China(31901985).Z.S.G.,Y.J.,C.Y.C.were funded by a Ningbo Science and Technology Research Grant(contract no 2016C10021).
文摘Red bayberry(Morella rubra)is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published.We updated the linkage map of the species by adding 118 SSR markers and the femalespecific marker MrFT2_BD-SEX.The integrated map included eight linkage groups and spanned 491 cM.Eleven sexassociated markers were identified,six of which were located in linkage group 8,in agreement with the previously reported location of the sex-determining region.The MrFT2_BD-SEX marker was genotyped in 203 cultivated accessions.Among the females of the accessions,we found two female-specific alleles,designated W-b(151 bp)and W-d(129 bp).We previously found that‘Dongkui’,a female cultivar,could produce viable pollen(we refer to such plants‘Dongkui-male’)and serve as the paternal parent in crosses.The genotypes of the MrFT2_BD-SEX marker were W-b/Z in‘Biqi’and W-d/Z in‘Dongkui-male’.The progeny of a cross between these parents produced a 3:1 female(W-)to male(ZZ)ratio and the expected 1:1:1:1 ratio of W-b/W-d:W-b/Z:W-d/Z:Z/Z.In addition,the flowering and fruiting phenotypes of all the F1 progeny fit their genotypes.Our results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus(W)in a small genomic region(59 kb and less than 3.3 cM).Furthermore,we have produced a homozygous“super female”(WW)that should produce all-female offspring in the F2 generation,providing a foundation for commercial use and presenting great potential for use in modern breeding programs.
基金supported by the National Natural Science Foundation of China(No.82104192)the Zhejiang Provincial Natural Science Foundation(No.LR22H310002)+1 种基金the Scientific Research Fund of Zhejiang University(No.XY2021044)the Zhejiang University K.P.Chao’s High Technology Development Foundation.
文摘A series of chemotherapeutic drugs that induce DNA damage,such as cisplatin(DDP),are standard clinical treatments for ovarian cancer,testicular cancer,and other diseases that lack effective targeted drug therapy.Drug resistance is one of the main factors limiting their application.Sensitizers can overcome the drug resistance of tumor cells,thereby enhancing the antitumor activity of chemotherapeutic drugs.In this study,we aimed to identify marketable drugs that could be potential chemotherapy sensitizers and explore the underlying mechanisms.We found that the alcohol withdrawal drug disulfiram(DSF)could significantly enhance the antitumor activity of DDP.JC-1 staining,propidium iodide(PI)staining,and western blotting confirmed that the combination of DSF and DDP could enhance the apoptosis of tumor cells.Subsequent RNA sequencing combined with Gene Set Enrichment Analysis(GSEA)pathway enrichment analysis and cell biology studies such as immunofluorescence suggested an underlying mechanism:DSF makes cells more vulnerable to DNA damage by inhibiting the Fanconi anemia(FA)repair pathway,exerting a sensitizing effect to DNA damaging agents including platinum chemotherapy drugs.Thus,our study illustrated the potential mechanism of action of DSF in enhancing the antitumor effect of DDP.This might provide an effective and safe solution for combating DDP resistance in clinical treatment.
基金supported by the Zhejiang Province Natural Science Foundation(LTGN24C020002)the National Natural Science Foundation of China(32101932,32072132)+3 种基金the Zhejiang Province Natural Science Foundation Joint Fund Project(LHZSD24C150001)the National Key R&D Program of China(2023YFD2300604)the Fundamental Research Funds for the Central Universities(226-2022-00215)Quzhou Characteristic Citrus Fruit Quality Detection Project(2023-KYY-516103-0008).
文摘Oral ulcers,a common mucosal affliction,severely diminish patients’quality of life due to pain and discomfort.Despite existing treatments,the frequent recurrence of oral ulcers underscores the need for more effective therapeutic options.This study aimed to investigate the inhibitory effects of Ponkan(Citrus reticulata‘Ponkan’)extracts and their flavonoid components on oral ulcers,exploring their potential as a natural therapy.Twenty different Ponkan cultivars were selected for flavonoid extraction and analysis.Ultra-performance liquid chromatography(UPLC)and high-resolution mass spectrometry(HRMS)were utilized for substance identification,leading to the discovery of 16 flavonoid compounds.Using an oral ulcer model established in Wistar rats,Ponkan extracts were found to effectively reduce ulcer area and pain sensitivity.The study revealed that four polymethoxyflavones(PMFs)were particularly effective in treating oral ulcers,while hesperidin did not show significant improvement.Mechanistic studies indicated that PMFs could inhibit the phosphorylation of Jak2 and Stat3,whereas hesperidin lacked this effect.This research supports the potential use of Ponkan and its flavonoids as a natural and effective alternative therapy for oral ulcers,with a focus on the role of PMFs in modulating the Jak2-Stat3 pathway,highlighting the possibilities for targeted natural treatments.
基金supported by the Guangzhou Science and Technology Program Project(No.202206010187 and 2023B03J1332)the National Natural Science Foundation of China(No.22208115)+1 种基金the Fundamental Research Funds for the Central Universities(No.2023ZYGXZR041)the Young Elite Scientists Sponsorship Program by CAST(No.2022QNRC001).
文摘To enhance the utilization of pesticides and reduce environmental risks,we constructed the magnetic recyclable and dual stimulus-responsive microspheres to achieve on-demand pesticide release.Magnetic multi-shell hollow mesoporous organosilicon nanoparticles(mMSN)were prepared by one-step hydrothermal method and loaded with pesticide avermectin(A@mMSN),afterward A@mMSN was coated with gelatin through emulsification and chemical cross-linking to prepare A@mMSN@G microspheres(21.5±9.7μm).After being absorbed by the pests,the gelatin layer was hydrolyzed with the neutral protease,and the disulfide bonds within mMSN framework were decomposed by glutathione(GSH),endowing A@mMSN@G microspheres with enzyme and GSH responsiveness to achieve sustained avermectin release till 7 days(about 3.5 times that of the commercial avermectin emulsion).Importantly,the A@mMSN@G microspheres containing Fe3O4 nanoparticles could be easily magnetically collected from soil with a recovery ratio of 63.7%,to reduce the environmental risks.With excellent biosafety,A@mMSN@G microspheres showed outstanding pest control effects till two weeks and the growth of cabbage was not affected by it.Therefore,based on the recyclability and dual stimulus-responsive controllable release,the fabricated A@mMSN@G microspheres have broad application potential in pesticide delivery.
