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甲烷氧化细菌(Methylosinus methanica)81Z中与甲醇氧化有关基因的克隆
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作者 张玉英 赵树杰 R.S.Hanson 《微生物学报》 CAS CSCD 北大核心 1991年第1期12-18,共7页
以大肠杆菌(E.coli)DH-1菌株和考斯质粒pLA 2917为宿主-载体系统,建立了甲烷氧化细菌(Methylosinus methanica)81Z染色体基因文库。甲醇利用菌(Methylobacterium organophilum)XX的甲醇脱氢酶(MDH)结构基因片段(2.5kb)经α-^(32)P标记... 以大肠杆菌(E.coli)DH-1菌株和考斯质粒pLA 2917为宿主-载体系统,建立了甲烷氧化细菌(Methylosinus methanica)81Z染色体基因文库。甲醇利用菌(Methylobacterium organophilum)XX的甲醇脱氢酶(MDH)结构基因片段(2.5kb)经α-^(32)P标记后作为探针对该文库进行筛选,得到两个杂交阳性重组质粒,即pG5和pH11,其中pG5的插入片段为25.4kb,互补分析表明:pG5的插入片段能使包括MDH和细胞色素C(Cyt.c)基因突变株在内的5株甲醇利用菌(Methylobacterium sp.)AM1的甲醇氧化阻滞突变株恢复在甲醇上的生长能力,并且能使3株甲醇利用菌XX的甲醇氧化阻滞突变株恢复野生型生长。至少有4个与甲醇氧化有关的基因位于该片段上。Southern blot杂交进一步证实:该插入片段与探针同源的区域在其4.8kb Sal Ⅰ片段内。 展开更多
关键词 甲烷氧化细菌 甲醇氧化 基因克隆
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Evaluation of Offshore Wastewater Outfall and Diffuser for Onondaga Lake, NY
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作者 Emmet M. Owens Steven W. Effler +1 位作者 David A. Matthews Anthony R. Prestigiacomo 《Journal of Water Resource and Protection》 2013年第9期1-15,共15页
Outfall alternatives are evaluated for a municipal wastewater treatment facility that discharges effluent at the shoreline of an urban lake. Occurrence of plumes of poorly diluted effluent in adjoining portions of the... Outfall alternatives are evaluated for a municipal wastewater treatment facility that discharges effluent at the shoreline of an urban lake. Occurrence of plumes of poorly diluted effluent in adjoining portions of the lake is described. Alternatives considered include outfalls over a range of depth and various diffuser designs. Benefits and impacts on lake stratification and dissolved oxygen are evaluated for an array of design alternatives with a model which links a far field hydrothermal and transport submodel with a near field buoyant plume submodel. Outfall design features are described that: 1) reduce shoreline discharge of bypass flow of partially treated wastewater during major runoff events;2) eliminate plumes of poorly diluted effluent;and 3) reduce loading of the effluent to the upper waters. A deep (10 to 14 m) outfall with a multiport diffuser would reduce the loading of the facility’s effluent to the upper waters by approximately 40%, without noteworthy impact on stratification or dissolved oxygen. 展开更多
关键词 Mathematical Model OUTFALL DIFFUSER Near Field DILUTION EUTROPHICATION
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Mask R-CNN aided fruit surface temperature monitoring algorithm with edge compute enabled internet of things system for automated apple heat stress management
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作者 Basavaraj R.Amogi Rakesh Ranjan Lav R.Khot 《Information Processing in Agriculture》 CSCD 2024年第4期603-611,共9页
Our prior study focused on development of internet of things(IoT)and edge-compute enabled crop physiology sensing system(CPSS)for apple sunburn monitoring.Edge compute algorithm on CPSS estimated sunburn susceptibilit... Our prior study focused on development of internet of things(IoT)and edge-compute enabled crop physiology sensing system(CPSS)for apple sunburn monitoring.Edge compute algorithm on CPSS estimated sunburn susceptibility as fruit surface temperature(FST)through pixel-by-pixel multiplication of captured thermal infrared images with segmented fruits binary mask.The segmentation was performed using color-based K means clustering approach.This limited CPSS applicability to monitor sunburn of red colored cultivars only and when fruits develop color,typically late growing season.This is a key research gap as recent weather patterns have shown that sunburn can occur during early growing season when fruits are green to yellow.Therefore,aim of this study was to develop and field evaluate cultivar and color independent mask region-convolution neural network(R-CNN)aided fruit segmentation model and edge compute compatible FST estimation algorithm.Season long field data were collected in 2021 using eight CPSS nodes(three in cv.WA38[Cosmic crisp]and five in cv.Honeycrisp).Collected data were used to develop and validate mask R-CNN based fruit segmentation model.Developed mask R-CNN based model was able to segment fruits of two apple cultivars and of varying colors with 91.4%average precision.In orchard evaluations(2022 season),the resulting algorithm ported on CPSS was able to accurately segment(dice similarity coefficient=0.89)and estimate apple FST with<0.5℃error compared to ground truth data.With compute time of about 37 s,data processing time was reduced by 22%over previous algorithm.High ambient temperature(>35℃)on a warmer day resulted in multiple throttling errors caused by excessive CPU temperature;however,the CPSS performance was uncompromised in FST estimation.Ambient air temperature did not affect RAM utilization and CPU clock frequency.Overall,developed FST algorithm can potentially be used as input to actuate water-based cooling system. 展开更多
关键词 Internet-of-Things Edge compute Deep learning Apple sunburn In-field sensing Fruit segmentation Fruit surface temperature
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Comparing the size selectivity of a novel T90 mesh codend to two conventional codends in the northern shrimp(Pandalus borealis)trawl fishery
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作者 Haraldur Arnar Einarsson Zhaohai Cheng +2 位作者 Shannon M.Bayse Bent Herrmann Paul DWinger 《Aquaculture and Fisheries》 2021年第4期382-392,共11页
The size selectivity and usability of three codends were quantified and compared for the first time in the inshore Northern shrimp(Pandalus borealis)trawl fishery of Iceland using the covered codend method:a conventio... The size selectivity and usability of three codends were quantified and compared for the first time in the inshore Northern shrimp(Pandalus borealis)trawl fishery of Iceland using the covered codend method:a conventional diamond-mesh codend(T0),conventional square-mesh codend(T45),and a 90◦turned mesh codend(T90)constructed of four panels and with shortened lastridge ropes.Fishers,wanting to increase the average-individual size of captured shrimp,had requested the T90 codend to be compared with conventional codends for consideration in the fishery.Results showed that,on average,the T45 and T90 codends had better size selectivity than the T0 codend in terms of releasing individuals smaller than 13 mm carapace length(Minimum References Size;MRS).The T90 codend retained significantly less Northern shrimps between 9 and 19 mm than the T0 codend and between 15 and 19 mm than the T45 codend.No significant difference of size selectivity between T45 and T0 codends was observed.All three codends presented high retention ratios of Northern shrimps above MRS(>63%)for the population encountered.However,the T0 codend was not effective at sorting out small Northern shrimps;at least 86%of Northern shrimps smaller than 13 mm were retained in the T0 codend if encountered.Catches from T45 and T90 codends had a lower proportion of shrimp below MRS.Since discarding of undersized Northern shrimps is prohibited in Iceland and fishers wanted to catch on average larger shrimp,using the novel T90 codend would enable fishers to use their quotas more efficiently. 展开更多
关键词 Size selectivity Codend usability Pandalus borealis Diamond-mesh T45 T90 Iceland Lastridge rope
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