Cornus wilsoniana W.is a woody oil plant with high oil content and strong hypolipidemic effects,making it a valuable species for medicinal,landscaping,and ecological purposes in China.To advance genetic research on th...Cornus wilsoniana W.is a woody oil plant with high oil content and strong hypolipidemic effects,making it a valuable species for medicinal,landscaping,and ecological purposes in China.To advance genetic research on this species,we employed PacBio together with Hi-C data to create a draft genome assembly for C.wilsoniana.Based on an 11-chromosome anchored chromosome-level assembly,the estimated genome size was determined to be 843.51 Mb.The N50 contig size and N50 scaffold size were calculated to be 4.49 and 78.00 Mb,respectively.Furthermore,30474 protein-coding genes were annotated.Comparative genomics analysis revealed that C.wilsoniana diverged from its closest species∼12.46 million years ago(Mya).Furthermore,the divergence between Cornaceae and Nyssaceae occurred>62.22 Mya.We also found evidence of whole-genome duplication events and whole-genome triplicationγ,occurring at∼44.90 and 115.86 Mya.We further inferred the origins of chromosomes,which sheds light on the complex evolutionary history of the karyotype of C.wilsoniana.Through transcriptional and metabolic analysis,we identified two FAD2 homologous genes that may play a crucial role in controlling the oleic to linoleic acid ratio.We further investigated the correlation between metabolites and genes and identified 33 MADS-TF homologous genes that may affect f lower morphology in C.wilsoniana.Overall,this study lays the groundwork for future research aimed at identifying the genetic basis of crucial traits in C.wilsoniana.展开更多
Article 59.1,of the International Code of Nomenclature for Algae,Fungi,and Plants(ICN;Melbourne Code),which addresses the nomenclature of pleomorphic fungi,became effective from 30 July 2011.Since that date,each funga...Article 59.1,of the International Code of Nomenclature for Algae,Fungi,and Plants(ICN;Melbourne Code),which addresses the nomenclature of pleomorphic fungi,became effective from 30 July 2011.Since that date,each fungal species can have one nomenclaturally correct name in a particular classification.All other previously used names for this species will be considered as synonyms.The older generic epithet takes priority over the younger name.Any widely used younger names proposed for use,must comply with Art.57.2 and their usage should be approved by the Nomenclature Committee for Fungi(NCF).In this paper,we list all genera currently accepted by us in Dothideomycetes(belonging to 23 orders and 110 families),including pleomorphic and nonpleomorphic genera.In the case of pleomorphic genera,we follow the rulings of the current ICN and propose single generic names for future usage.The taxonomic placements of 1261 genera are listed as an outline.Protected names and suppressed names for 34 pleomorphic genera are listed separately.Notes and justifications are provided for possible proposed names after the list of genera.Notes are also provided on recent advances in our understanding of asexual and sexual morph linkages in Dothideomycetes.A phylogenetic tree based on four gene analyses supported 23 orders and 75 families,while 35 families still lack molecular data.展开更多
During a survey on corticolous Dothideomycetes,several collections with ascospores matching the genera Asteromassaria and Stigmatomassaria(Pleomassariaceae,Pleosporales)were revealed from dead corticated twigs of Acer...During a survey on corticolous Dothideomycetes,several collections with ascospores matching the genera Asteromassaria and Stigmatomassaria(Pleomassariaceae,Pleosporales)were revealed from dead corticated twigs of Acer,Carpinus and Tamarix.Closer morphological examination showed that their ascomata were apothecial,with a hamathecium consisting of septate,branched paraphyses,which are apically swollen at maturity.Several collections were cultured and sequenced,and a Blast search of their nuc 28S rDNA sequences revealed dothideomycetous affiliation,but without a close match to a specific family or order.Phylogenetic analyses of a multigene matrix containing a representative selection of Dothideomycetes from four genes(nuc 18S rDNA,nuc 28S rDNA,rpb2 and tef1)revealed placement within Dothideomycetes but without a supported familial or ordinal affiliation.Based on the phylogenetic analyses and morphological investigations,the new genera Asterodiscus and Stigmatodiscus,with the two new species A.tamaricis and S.enigmaticus,are described and illustrated,and placed in the new family Stigmatodiscaceae and new order Stigmatodiscales.展开更多
More than 100 recent collections of Valsaria sensu lato mostly from Europe were used to elucidate the species composition within the genus.Multigene phylogeny based on SSU,LSU,ITS,rpb2 and tef1 sequences revealed a mo...More than 100 recent collections of Valsaria sensu lato mostly from Europe were used to elucidate the species composition within the genus.Multigene phylogeny based on SSU,LSU,ITS,rpb2 and tef1 sequences revealed a monophyletic group of ten species within the Dothideomycetes,belonging to three morphologically similar genera.This group could not be accommodated in any known family and are thus classified in the new family Valsariaceae and the new order Valsariales.The genus Valsaria sensu stricto comprises V.insitiva,V.robiniae,V.rudis,V.spartii,V.lopadostomoides sp.nov.and V.neotropica sp.nov.,which are phylogenetically well-defined,but morphologically nearly indistinguishable species.The new monotypic genus Bambusaria is introduced to accommodate Valsaria bambusae.Munkovalsaria rubra and Valsaria fulvopruinata are combined in Myrmaecium,a genus traditionally treated as a synonym of Valsaria,which comprises three species,with M.rubricosum as its generic type.This work is presented as a basis for additional species to be detected in future.展开更多
基金supported by the National Natural Science Foundation of China(31770767,32070656,32070677 and 32270709)Foundation of State Key Laboratory of Utilization of Woody Oil Resource(GZKF202201)+1 种基金Hunan Province Key Research and Development Program Project(2018NK2044)Jiangsu Collaborative Innovation Center for Modern Crop Production and Collaborative Innovation Center for Modern Crop Production cosponsored by province and ministry.
文摘Cornus wilsoniana W.is a woody oil plant with high oil content and strong hypolipidemic effects,making it a valuable species for medicinal,landscaping,and ecological purposes in China.To advance genetic research on this species,we employed PacBio together with Hi-C data to create a draft genome assembly for C.wilsoniana.Based on an 11-chromosome anchored chromosome-level assembly,the estimated genome size was determined to be 843.51 Mb.The N50 contig size and N50 scaffold size were calculated to be 4.49 and 78.00 Mb,respectively.Furthermore,30474 protein-coding genes were annotated.Comparative genomics analysis revealed that C.wilsoniana diverged from its closest species∼12.46 million years ago(Mya).Furthermore,the divergence between Cornaceae and Nyssaceae occurred>62.22 Mya.We also found evidence of whole-genome duplication events and whole-genome triplicationγ,occurring at∼44.90 and 115.86 Mya.We further inferred the origins of chromosomes,which sheds light on the complex evolutionary history of the karyotype of C.wilsoniana.Through transcriptional and metabolic analysis,we identified two FAD2 homologous genes that may play a crucial role in controlling the oleic to linoleic acid ratio.We further investigated the correlation between metabolites and genes and identified 33 MADS-TF homologous genes that may affect f lower morphology in C.wilsoniana.Overall,this study lays the groundwork for future research aimed at identifying the genetic basis of crucial traits in C.wilsoniana.
基金the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botanya research grant from the Biodiversity Research and Training Program(BRT R253012)+2 种基金The Thailand Research Fund(BRG 5280002)The International Scientific Cooperated Project of Guizhou Province(No[2013]7004)funding from the Spanish Ministerio de Ciencia e Innovación project CGL2011-25003.
文摘Article 59.1,of the International Code of Nomenclature for Algae,Fungi,and Plants(ICN;Melbourne Code),which addresses the nomenclature of pleomorphic fungi,became effective from 30 July 2011.Since that date,each fungal species can have one nomenclaturally correct name in a particular classification.All other previously used names for this species will be considered as synonyms.The older generic epithet takes priority over the younger name.Any widely used younger names proposed for use,must comply with Art.57.2 and their usage should be approved by the Nomenclature Committee for Fungi(NCF).In this paper,we list all genera currently accepted by us in Dothideomycetes(belonging to 23 orders and 110 families),including pleomorphic and nonpleomorphic genera.In the case of pleomorphic genera,we follow the rulings of the current ICN and propose single generic names for future usage.The taxonomic placements of 1261 genera are listed as an outline.Protected names and suppressed names for 34 pleomorphic genera are listed separately.Notes and justifications are provided for possible proposed names after the list of genera.Notes are also provided on recent advances in our understanding of asexual and sexual morph linkages in Dothideomycetes.A phylogenetic tree based on four gene analyses supported 23 orders and 75 families,while 35 families still lack molecular data.
基金The financial support by the Austrian Science Fund(FWF,project P25870-B16)to WJ is gratefully acknowledgedWe thank Conrad Schoch for providing the matrix of Dothideomycetes,Irmgard Greilhuber and Walter Gams for excursion support and especially R.Rousseaux for collection of AG13137.
文摘During a survey on corticolous Dothideomycetes,several collections with ascospores matching the genera Asteromassaria and Stigmatomassaria(Pleomassariaceae,Pleosporales)were revealed from dead corticated twigs of Acer,Carpinus and Tamarix.Closer morphological examination showed that their ascomata were apothecial,with a hamathecium consisting of septate,branched paraphyses,which are apically swollen at maturity.Several collections were cultured and sequenced,and a Blast search of their nuc 28S rDNA sequences revealed dothideomycetous affiliation,but without a close match to a specific family or order.Phylogenetic analyses of a multigene matrix containing a representative selection of Dothideomycetes from four genes(nuc 18S rDNA,nuc 28S rDNA,rpb2 and tef1)revealed placement within Dothideomycetes but without a supported familial or ordinal affiliation.Based on the phylogenetic analyses and morphological investigations,the new genera Asterodiscus and Stigmatodiscus,with the two new species A.tamaricis and S.enigmaticus,are described and illustrated,and placed in the new family Stigmatodiscaceae and new order Stigmatodiscales.
基金We sincerely thank F.Candoussau,G.Friebes,A.Gardiennet,J.-R.Guu,H.-M.Hsieh,P.Karasch,C.Lechat,M.Pélissier,F.Rappaz and J.D.Rogers for providing specimens,Y.-M.Ju for specimens,cultures and helpful comments on the manuscript,W.Gams for excursion support in Italy,F.Balao,J.Herrera and S.Tello for support and help with determination of fabaceous hosts in Spainthe fungarium curators S.Dominick and E.Roark(BPI),P.Salo(H),B.Aguirre-Hudson(K),N.Sol and G.Thijsse(L),E.Bloch(NY),B.Buyck(PC),A.Freire-Fierro and N.Phillips(PH)for loans and information of specimens,W.Till at WU for sending and managing collections+2 种基金T.Merkx(CBS)for managing our cultures,G.Verkley(CBS)for providing cultures and information about strains of M.rubrumand W.Dämon and I.Greilhuber for insertion of specimens into WU.The financial support by the Austrian Science Fund(FWFproject P25870-B16)is gratefully acknowledged.K.D.Hyde and D.Q.Dai thank the Mae Fah Luang University for the grant BTaxonomy and Phylogeny of selected families of Dothideomycetes(Grant number:56101020032).D.Q.Dai is grateful to the Mushroom Research Foundation,Bandoo,Chiang Rai Province,Thailand,for providing postgraduate scholarship support and thanks the Mae Fah Luang University for the GMS grant.
文摘More than 100 recent collections of Valsaria sensu lato mostly from Europe were used to elucidate the species composition within the genus.Multigene phylogeny based on SSU,LSU,ITS,rpb2 and tef1 sequences revealed a monophyletic group of ten species within the Dothideomycetes,belonging to three morphologically similar genera.This group could not be accommodated in any known family and are thus classified in the new family Valsariaceae and the new order Valsariales.The genus Valsaria sensu stricto comprises V.insitiva,V.robiniae,V.rudis,V.spartii,V.lopadostomoides sp.nov.and V.neotropica sp.nov.,which are phylogenetically well-defined,but morphologically nearly indistinguishable species.The new monotypic genus Bambusaria is introduced to accommodate Valsaria bambusae.Munkovalsaria rubra and Valsaria fulvopruinata are combined in Myrmaecium,a genus traditionally treated as a synonym of Valsaria,which comprises three species,with M.rubricosum as its generic type.This work is presented as a basis for additional species to be detected in future.
