Cronobacter sakazakii(C.sakazakii)is a foodborne opportunistic pathogen that can cause life-threatening invasive diseases,such as necrotizing enterocolitis,meningitis,and sepsis in infants.The potential risk of C.saka...Cronobacter sakazakii(C.sakazakii)is a foodborne opportunistic pathogen that can cause life-threatening invasive diseases,such as necrotizing enterocolitis,meningitis,and sepsis in infants.The potential risk of C.sakazakii contamination of powdered infant formula(PIF)is an issue that has attracted considerable attention from manufacturers,regulators,and consumers.C.sakazakii biofilms on the surfaces of equipment and in diverse food-production environments constitute a mode of cell growth that protects the pathogen from hostile environments,and are an important source of persistent contamination of food products.Bacterial biofilms are difficult to remove due to their resistant properties.Conventional cleaning and sterilizing procedures may be insufficient for biofilm control,and may lead to further biofilm development and dispersal.Consequently,novel control strategies are being developed,such as nanotechnology-based delivery systems,interspecies interactions,antimicrobial molecules of microbial origin,natural extracts,and phages.This review focuses on describing the mechanisms underlying the biofilm formation and behavior of C.sakazakii and discussing potential control strategies.展开更多
Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in ...Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in Cronobacter has increased significantly in recent years due to its high virulence in children.In this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food types.We discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its pathogenicity.We emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments.展开更多
Biofilms containing foodborne pathogens can adhere to food ingredients or food processing equipment.Due to their enhanced resilience to cleaning and chance of cross-contamination,there is an increased risk of food poi...Biofilms containing foodborne pathogens can adhere to food ingredients or food processing equipment.Due to their enhanced resilience to cleaning and chance of cross-contamination,there is an increased risk of food poisoning to consumers.The life course of biofilms is generally thought to be regulated by a quorum sensing(QS)system by secreted autoinducing peptides(AIPs).Recent studies have revealed that small non-coding RNA(sRNA)also plays an important role in regulating biofilm formation at the post-transcriptional level.Moreover,sRNA can act as an intermediate regulating hub between the quorum sensing system and biofilm.This review summarizes the latest findings on sRNA in biofilms of common foodborne pathogens and their potential impact.We also highlight the different mechanism of cascade regulation of sRNA and QS system in biofilm formation in several typical foodborne pathogens.In this process,sRNA responds to dynamic external environmental signals finely and accurately,and can feedback information on the bacterial cell environment to modify the QS system.These findings will deepen our understanding of biofilm formation and provide new perspectives for eliminating biofilm of foodborne pathogens based on the cascade regulation of small RNA and QS for developing biofilm-free food-processing systems.展开更多
基金financial support of National Key Research and Development Program of China(2017YFC1601200)the Science and Technology Planning Project of Guangdong Province(2017A070702018)+1 种基金the Science and Technology Planning Project of Guangzhou(201604020003)Guangdong Academy of Sciences Special Project of Science and Technology Development(2017GDASCX-0201).
文摘Cronobacter sakazakii(C.sakazakii)is a foodborne opportunistic pathogen that can cause life-threatening invasive diseases,such as necrotizing enterocolitis,meningitis,and sepsis in infants.The potential risk of C.sakazakii contamination of powdered infant formula(PIF)is an issue that has attracted considerable attention from manufacturers,regulators,and consumers.C.sakazakii biofilms on the surfaces of equipment and in diverse food-production environments constitute a mode of cell growth that protects the pathogen from hostile environments,and are an important source of persistent contamination of food products.Bacterial biofilms are difficult to remove due to their resistant properties.Conventional cleaning and sterilizing procedures may be insufficient for biofilm control,and may lead to further biofilm development and dispersal.Consequently,novel control strategies are being developed,such as nanotechnology-based delivery systems,interspecies interactions,antimicrobial molecules of microbial origin,natural extracts,and phages.This review focuses on describing the mechanisms underlying the biofilm formation and behavior of C.sakazakii and discussing potential control strategies.
基金the financial support of National Key Research and Development program, China (2017YFC1601202 and 2017YFC1601200)China Postdoctoral Science Foundation (2021M690854)+2 种基金National Natural Science Foundation of China (31671951 and 31972175)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (2017BT01S174)Fundamental Research Funds for the Central Universities, China (JZ2020HGQA0151)
文摘Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in Cronobacter has increased significantly in recent years due to its high virulence in children.In this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food types.We discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its pathogenicity.We emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments.
基金This work was supported by National Natural Science Foundation of China(32272424)Guangdong Major Project of Basic and Applied Basic Research(2020B0301030005)+1 种基金Guangdong Provincial Key Laboratory(2020B121201009)Guangdong Academy of Sciences Special Project for Capacity Building of Innovation Driven Development(2020GDASYL-20200401002).
文摘Biofilms containing foodborne pathogens can adhere to food ingredients or food processing equipment.Due to their enhanced resilience to cleaning and chance of cross-contamination,there is an increased risk of food poisoning to consumers.The life course of biofilms is generally thought to be regulated by a quorum sensing(QS)system by secreted autoinducing peptides(AIPs).Recent studies have revealed that small non-coding RNA(sRNA)also plays an important role in regulating biofilm formation at the post-transcriptional level.Moreover,sRNA can act as an intermediate regulating hub between the quorum sensing system and biofilm.This review summarizes the latest findings on sRNA in biofilms of common foodborne pathogens and their potential impact.We also highlight the different mechanism of cascade regulation of sRNA and QS system in biofilm formation in several typical foodborne pathogens.In this process,sRNA responds to dynamic external environmental signals finely and accurately,and can feedback information on the bacterial cell environment to modify the QS system.These findings will deepen our understanding of biofilm formation and provide new perspectives for eliminating biofilm of foodborne pathogens based on the cascade regulation of small RNA and QS for developing biofilm-free food-processing systems.
