Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluor...Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.展开更多
为开发新型高品质的蜂蜜茶酒,利用酿酒酵母和非酿酒酵母混菌发酵制备蜂蜜茶酒,采用电子鼻技术结合顶空固相微萃取-气相色谱-质谱(Headspace Solid-Phase Micro Extraction and Gas Chromatography Mass Spectrometry,HS-SPME-GC-MS),并...为开发新型高品质的蜂蜜茶酒,利用酿酒酵母和非酿酒酵母混菌发酵制备蜂蜜茶酒,采用电子鼻技术结合顶空固相微萃取-气相色谱-质谱(Headspace Solid-Phase Micro Extraction and Gas Chromatography Mass Spectrometry,HS-SPME-GC-MS),并对发酵蜂蜜茶酒的基本理化、有机酸和多酚含量进行检测,以酿酒酵母单菌发酵(SF)和非酿酒酵母单菌发酵(HF)产品作为对比分析,综合评价混菌发酵(MF)对蜂蜜茶酒品质和风味的影响。结果表明:相较于SF和HF,MF提高了蜂蜜茶酒总酚和总黄酮含量。发酵后样品的有机酸含量上升,多酚含量下降。HF电子鼻传感器响应值低,且MF大部分传感器响应值大于SF。HS-SPME-GC-MS共鉴定出75种香气成分,混菌发酵使蜂蜜茶酒中的挥发性化合物种类和含量显著增加(P<0.05)。PLS-DA分析共筛选出10种差异化合物(VIP>1),其中有6种为酯类风味物质。OAV>1的关键香气成分共有12种,相较于HF和SF,对MF香气贡献率较高的为具有花香的苯乙醇、蜂蜜甜香的乙酸苯乙酯、水果香气的甲酸戊酯和醋酸正丙酯等。综上,混菌发酵蜂蜜茶酒相较于单菌发酵更具优势,总体风味更丰富浓郁。本文为非酿酒酵母在酒类中的应用及茶叶副产物深加工利用提供了理论依据。展开更多
Pt(Ⅱ)-salophen complexes(S-1~S-4) and 9,10-diphenylanthracene(DPA) tethering pillar[5]arene derivatives(A-1 and A-2) were synthesized to act as sensitizers and annihilators for triplet-triplet annihilation upconversi...Pt(Ⅱ)-salophen complexes(S-1~S-4) and 9,10-diphenylanthracene(DPA) tethering pillar[5]arene derivatives(A-1 and A-2) were synthesized to act as sensitizers and annihilators for triplet-triplet annihilation upconversion(TTA-UC), respectively. It turned out that the pyridine cation served as a mask for the excited state of the sensitizer, the triplet states of S-2 and S-3 were significantly quenched by photo-induced electron transfer(PET) with phosphorescence quantum yield quenched from 24.4% for S-4 to 9.3% for S-3,and therefore, both S-2 and S-3 led to negligible UC emissions when traditional annihilator DPA was used as the annihilator. Delightfully, when supramolecular annihilator A-1 and A-2 were employed to include the pyridine cation, PET was significantly inhibited and the triplet states of the sensitizers were activated,TTA-UC emission was therefore boosted. The UC quantum yield of A-2/S-3 system was up to 130 times higher than that of DPA/S-3 system, and the UC emission was switchable by the addition of competitive vips.展开更多
Depression is a prevalent mental disorder with limited effective treatments,posing a significant global issue.This study explored L-theanine and geniposide,key components in“food-medicine homology”materials,to deter...Depression is a prevalent mental disorder with limited effective treatments,posing a significant global issue.This study explored L-theanine and geniposide,key components in“food-medicine homology”materials,to determine if their combination(TG)could alleviate depression-like behaviors and hippocampal neuronal damage in a chronic unpredictable mild stress(CUMS)mouse model.Male C57BL/6J mice were divided into control,CUMS model,and CUMS+TG groups with varying doses.The CUMS group displayed depressionlike behaviors,including reduced activity and sucrose preference.TG treatment partially reversed these changes,significantly increasing antioxidant enzyme activities,decreasing pro-inflammatory cytokine levels,and improving neuromodulator levels.RNA-seq analysis identified the transthyretin(TTR)gene,upregulated in the model group but downregulated after TG treatment.TG treatment modulated intestinal microbiota composition compared to the CUMS group,including increased Firmicutes,reduced Bacteroidetes and Prevotella,and variable changes in Bifidobacterium abundance.In conclusion,our study indicates that CUMS exposure upregulates stress hormones and TTR expression,associated with neuroinflammation,oxidative stress,monoamine depletion,depression-like behaviors,and intestinal microbiota dysbiosis.TG treatment alleviates these effects and modulates intestinal microbiota,suggesting L-theanine and geniposide's potential as a novel depression therapy.展开更多
The impacts of drought stress on crop yield and quality are substantial. Drought priming during the early growth stage of plants has been shown to improve tolerance to drought stress during the reproductive stage, alt...The impacts of drought stress on crop yield and quality are substantial. Drought priming during the early growth stage of plants has been shown to improve tolerance to drought stress during the reproductive stage, although its effects on grain quality remain elusive. This study investigated the influence of drought priming on starch and protein levels in grains under drought stress during grain filling. Our results revealed that drought stress leads to a reduction in the contents of starch and its constituents, while simultaneously increasing glutenin macropolymers and protein fractions. Notably, drought primed plants under drought stress(PD) exhibited mitigated declines in the contents of starch and its components, leading to improvements in starch swelling power and pasting properties. In addition, PD resulted in a slight increase in the protein fractions, limiting the overall rise in total protein content compared to drought stress alone. The results of our study underscore the efficacy of drought priming as a strategy to counteract the negative effects of drought stress on grain quality, particularly by minimizing starch losses and restraining protein content elevation.展开更多
基金financially supported by National Natural Science Foundation of China(22074100)the Young Elite Scientist Sponsorship Program by CAST(YESS20200036)+3 种基金the Researchers Supporting Project Number RSP-2021/138King Saud University,Riyadh,Saudi ArabiaTechnological Innovation R&D Project of Chengdu City(2019-YF05-31702266-SN)Sichuan University-Panzhihua City joint Project(2020CDPZH-5)。
文摘Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.
文摘为开发新型高品质的蜂蜜茶酒,利用酿酒酵母和非酿酒酵母混菌发酵制备蜂蜜茶酒,采用电子鼻技术结合顶空固相微萃取-气相色谱-质谱(Headspace Solid-Phase Micro Extraction and Gas Chromatography Mass Spectrometry,HS-SPME-GC-MS),并对发酵蜂蜜茶酒的基本理化、有机酸和多酚含量进行检测,以酿酒酵母单菌发酵(SF)和非酿酒酵母单菌发酵(HF)产品作为对比分析,综合评价混菌发酵(MF)对蜂蜜茶酒品质和风味的影响。结果表明:相较于SF和HF,MF提高了蜂蜜茶酒总酚和总黄酮含量。发酵后样品的有机酸含量上升,多酚含量下降。HF电子鼻传感器响应值低,且MF大部分传感器响应值大于SF。HS-SPME-GC-MS共鉴定出75种香气成分,混菌发酵使蜂蜜茶酒中的挥发性化合物种类和含量显著增加(P<0.05)。PLS-DA分析共筛选出10种差异化合物(VIP>1),其中有6种为酯类风味物质。OAV>1的关键香气成分共有12种,相较于HF和SF,对MF香气贡献率较高的为具有花香的苯乙醇、蜂蜜甜香的乙酸苯乙酯、水果香气的甲酸戊酯和醋酸正丙酯等。综上,混菌发酵蜂蜜茶酒相较于单菌发酵更具优势,总体风味更丰富浓郁。本文为非酿酒酵母在酒类中的应用及茶叶副产物深加工利用提供了理论依据。
基金supported by the National Natural Science Foundation of China (Nos. 22171194, 21971169, 92056116 and 21871194)the Fundamental Research Funds for the Central Universities (No. 20826041D4117)the Science & Technology Department of Sichuan Province (Nos. 2022YFH0095 and 2021ZYD0052)。
文摘Pt(Ⅱ)-salophen complexes(S-1~S-4) and 9,10-diphenylanthracene(DPA) tethering pillar[5]arene derivatives(A-1 and A-2) were synthesized to act as sensitizers and annihilators for triplet-triplet annihilation upconversion(TTA-UC), respectively. It turned out that the pyridine cation served as a mask for the excited state of the sensitizer, the triplet states of S-2 and S-3 were significantly quenched by photo-induced electron transfer(PET) with phosphorescence quantum yield quenched from 24.4% for S-4 to 9.3% for S-3,and therefore, both S-2 and S-3 led to negligible UC emissions when traditional annihilator DPA was used as the annihilator. Delightfully, when supramolecular annihilator A-1 and A-2 were employed to include the pyridine cation, PET was significantly inhibited and the triplet states of the sensitizers were activated,TTA-UC emission was therefore boosted. The UC quantum yield of A-2/S-3 system was up to 130 times higher than that of DPA/S-3 system, and the UC emission was switchable by the addition of competitive vips.
基金funded by Innovation Project for Chinese Academy of Agricultural Sciences(CAAS-ASTIP-TRI)China Agriculture Research System of MOF and MARA(CARS-19)。
文摘Depression is a prevalent mental disorder with limited effective treatments,posing a significant global issue.This study explored L-theanine and geniposide,key components in“food-medicine homology”materials,to determine if their combination(TG)could alleviate depression-like behaviors and hippocampal neuronal damage in a chronic unpredictable mild stress(CUMS)mouse model.Male C57BL/6J mice were divided into control,CUMS model,and CUMS+TG groups with varying doses.The CUMS group displayed depressionlike behaviors,including reduced activity and sucrose preference.TG treatment partially reversed these changes,significantly increasing antioxidant enzyme activities,decreasing pro-inflammatory cytokine levels,and improving neuromodulator levels.RNA-seq analysis identified the transthyretin(TTR)gene,upregulated in the model group but downregulated after TG treatment.TG treatment modulated intestinal microbiota composition compared to the CUMS group,including increased Firmicutes,reduced Bacteroidetes and Prevotella,and variable changes in Bifidobacterium abundance.In conclusion,our study indicates that CUMS exposure upregulates stress hormones and TTR expression,associated with neuroinflammation,oxidative stress,monoamine depletion,depression-like behaviors,and intestinal microbiota dysbiosis.TG treatment alleviates these effects and modulates intestinal microbiota,suggesting L-theanine and geniposide's potential as a novel depression therapy.
基金supported by the projects of the National Key Research and Development Program of China (2023YFD2300202)the National Natural Science Foundation of China (32272213 and 31771693)+1 种基金the China Agriculture Research System (CARS-03)the Jiangsu Collaborative Innovation Center for Modern Crop Production, China (JCIC-MCP)。
文摘The impacts of drought stress on crop yield and quality are substantial. Drought priming during the early growth stage of plants has been shown to improve tolerance to drought stress during the reproductive stage, although its effects on grain quality remain elusive. This study investigated the influence of drought priming on starch and protein levels in grains under drought stress during grain filling. Our results revealed that drought stress leads to a reduction in the contents of starch and its constituents, while simultaneously increasing glutenin macropolymers and protein fractions. Notably, drought primed plants under drought stress(PD) exhibited mitigated declines in the contents of starch and its components, leading to improvements in starch swelling power and pasting properties. In addition, PD resulted in a slight increase in the protein fractions, limiting the overall rise in total protein content compared to drought stress alone. The results of our study underscore the efficacy of drought priming as a strategy to counteract the negative effects of drought stress on grain quality, particularly by minimizing starch losses and restraining protein content elevation.
