Objective:To explore factors influencing decision regret among colorectal cancer patients undergoing intestinal ostomy.Methods:A questionnaire survey was conducted among 102 colorectal cancer patients who underwent in...Objective:To explore factors influencing decision regret among colorectal cancer patients undergoing intestinal ostomy.Methods:A questionnaire survey was conducted among 102 colorectal cancer patients who underwent intestinal ostomy surgery and visited the ostomy clinic at a tertiary hospital in Baoding from July to September 2025.The Chinese version of the Ostomy Adaptation Inventory(OAI-20),Decision Regret Scale(DRS),Decision Conflict Scale(DCS),and Functional Assessment of Cancer Therapy-Colorectal(FACT-C)were used to measure patients’adaptation to stoma,decision regret,decision conflict,and quality of life.The Shared Decision-Making Questionnaire(SDM-Q-9)assessed patient involvement in ostomy surgery decisions,while the SSUK-8 evaluated social support.Additional items explored perceptions related to decision-making,participation,and outcomes.Results:Among 134 eligible patients attending the clinic,120 participated in the questionnaire,with 102 completing all items.Stoma patients reported an average decision regret score of 60.83(SD 28.43),an average coping ability score of 54.26(SD 26.69),an average decision conflict score of 62.55(SD 25.95),and a quality of life score of 56.93(SD 27.46).In the multiple regression analysis,decision regret was associated with decision conflict,poor patient coping ability,low quality of life,and low social support.Conclusion:Decision regret is prevalent among Chinese CRC patients following ostomy surgery.Compared with similar studies in other regions,Chinese CRC patients exhibit a higher rate of regret.This may be related to lower patient involvement in decision-making,generally poorer quality of life,and heavier economic burdens.展开更多
The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5（RGC-5） necroptosis following oxygen glucose deprivation（OGD）. RGC-5 cells were cultured in Dulbecco＇s-mo...The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5（RGC-5） necroptosis following oxygen glucose deprivation（OGD）. RGC-5 cells were cultured in Dulbecco＇s-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal（ALLN） pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor（t AIF） in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and t AIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule t AIF.展开更多
文摘Objective:To explore factors influencing decision regret among colorectal cancer patients undergoing intestinal ostomy.Methods:A questionnaire survey was conducted among 102 colorectal cancer patients who underwent intestinal ostomy surgery and visited the ostomy clinic at a tertiary hospital in Baoding from July to September 2025.The Chinese version of the Ostomy Adaptation Inventory(OAI-20),Decision Regret Scale(DRS),Decision Conflict Scale(DCS),and Functional Assessment of Cancer Therapy-Colorectal(FACT-C)were used to measure patients’adaptation to stoma,decision regret,decision conflict,and quality of life.The Shared Decision-Making Questionnaire(SDM-Q-9)assessed patient involvement in ostomy surgery decisions,while the SSUK-8 evaluated social support.Additional items explored perceptions related to decision-making,participation,and outcomes.Results:Among 134 eligible patients attending the clinic,120 participated in the questionnaire,with 102 completing all items.Stoma patients reported an average decision regret score of 60.83(SD 28.43),an average coping ability score of 54.26(SD 26.69),an average decision conflict score of 62.55(SD 25.95),and a quality of life score of 56.93(SD 27.46).In the multiple regression analysis,decision regret was associated with decision conflict,poor patient coping ability,low quality of life,and low social support.Conclusion:Decision regret is prevalent among Chinese CRC patients following ostomy surgery.Compared with similar studies in other regions,Chinese CRC patients exhibit a higher rate of regret.This may be related to lower patient involvement in decision-making,generally poorer quality of life,and heavier economic burdens.
基金supported by grants from National Natural Science Foundation of China(No.81371011,and No.81571939)Wu Jie-Ping Medical Foundation of the Minister of Health of China(No.320.6750.14118)+3 种基金Natural Science Foundation of Hunan Province(No.2015JJ2187)Teacher Research Foundation of Central South University(No.2014JSJJ026)Science Foundation for the Youth Scholars of Central South University(No.120973)Project of Innovation-driven Plan of Central South University(No.2015CXS022)
文摘The purpose of this study was to investigate the effect of inhibition of calpain on retinal ganglion cell-5（RGC-5） necroptosis following oxygen glucose deprivation（OGD）. RGC-5 cells were cultured in Dulbecco＇s-modified essential medium and necroptosis was induced by 8-h OGD. PI staining and flow cytometry were performed to detect RGC-5 necrosis. The calpain expression was detected by Western blotting and immunofluorescence staining. The calpain activity was tested by activity detection kit. Flow cytometry was used to detect the effect of calpain on RGC-5 necroptosis following OGD with or without N-acetyl-leucyl-leucyl-norleucinal（ALLN） pre-treatment. Western blot was used to detect the protein level of truncated apoptosis inducing factor（t AIF） in RGC-5 cells following OGD. The results showed that there was an up-regulation of the calpain expression and activity following OGD. Upon adding ALLN, the calpain activity was inhibited and t AIF was reduced following OGD along with the decreased number of RGC-5 necroptosis. In conclusion, calpain was involved in OGD-induced RGC-5 necroptosis with the increased expression of its downstream molecule t AIF.
