Objective: To investigate the effect of a self-developed balance rhythm dance program on the rehabilitation of motor function and the reduction of fall risk in elderly women with diminished balance function. Methods: ...Objective: To investigate the effect of a self-developed balance rhythm dance program on the rehabilitation of motor function and the reduction of fall risk in elderly women with diminished balance function. Methods: Fifty elderly women with reduced balance function, admitted to the Qingbar Elderly Care Center of Chongqing Medical University from December 2022 to December 2023, were randomly selected and divided into two groups. The aerobic exercise group (25 patients) received traditional treatment and rehabilitation nursing, while the balance rhythm dance intervention group (25 patients) received the balance rhythm dance intervention in addition to traditional treatment and rehabilitation nursing. The Unified Parkinson’s Disease Rating Scale (UPDRS) and Berg Balance Scale (BBS) were used as evaluation indicators to compare the intervention effects between the two groups. Results: The data revealed that the balance rhythm dance intervention significantly improved the motor ability and balance function of elderly women in the intervention group (P < 0.01), with statistically significant differences observed. Conclusion: The balance rhythm dance program plays a critical role in promoting the rehabilitation of motor function and balance ability in elderly women, effectively enhancing their quality of life.展开更多
Proton nuclear(^(1)H)is the observed nucleus on which most magnetic resonance imaging(MRI)applications depend.Most traditional^(1)H MRI can provide structural and functional information about organisms,while various n...Proton nuclear(^(1)H)is the observed nucleus on which most magnetic resonance imaging(MRI)applications depend.Most traditional^(1)H MRI can provide structural and functional information about organisms,while various non-proton nuclei(X-nuclei)MRI can provide more metabolic information.However,due to the relatively poor signal-to-noise ratio(SNR)of X-nuclei MRI,their applications are quite rare compared to^(1)H.Benefit from the rapid developments of MRI hardware and software technologies,X-nuclei MRI has recently attracted increasing interests in biomedical research.This review firstly introduces some current methods to improve the SNR of X-nuclei MRI.Secondly,this review describes biomedical applications of X-nuclei MRI,especially focusing on the current use of X-nuclei(^(13)C,^(17)O,^(19)F,^(23)Na and^(31)P)MRI to study related diseases in different organs,including the brain,liver,kidney,heart and bone.Finally,perspectives studies on X-nuclei imaging and its potential applications are described in biomedical research.展开更多
In analyses of protein families that may serve as drug targets,membrane-associated G-protein-coupled receptors(GPCRs)dominate,followed by ion channels,transporters,and—to a lesser extent—membrane-bound enzymes.Howev...In analyses of protein families that may serve as drug targets,membrane-associated G-protein-coupled receptors(GPCRs)dominate,followed by ion channels,transporters,and—to a lesser extent—membrane-bound enzymes.However,various challenges put such membrane proteins among key groups of underutilized opportunities for the application of therapeutic antibodies.Antibodies hold the promise of exquisite specificity,as they are able to target even specific conformations of a particular membrane protein,as well as adaptability through engineering into various antibody formats.However,the ease of raising and isolating specific,effective antibodies targeting membrane proteins depends on many factors.In particular,the generation of specific antibodies is easier when targeting larger,simpler,extracellular domains with greater uniqueness of amino acid sequence.The rareness of such ideal conditions is illustrated by the limited number of approved biologics for targeting GPCRs and other complex membrane proteins.Challenges in developing antibodies to complex membrane proteins such as GPCRs,ion channels,transporters,and membrane-bound enzymes can be addressed by the design of the antigen,antibody-generation strategies,lead optimization technologies,and antibody modalities.A better understanding of the membrane proteins being targeted would facilitate mechanism-based drug discovery.This review describes the advantages and challenges of targeting complex membrane proteins with antibodies and discusses the preparation of membrane protein antigens and antibody generation,illustrated by select examples of success.展开更多
Objective： The Chinese Quality of Life Instrument （ChQOL） was developed as a valid generic health status instrument based on the well-established theory of health in Chinese medicine. Psychometric properties of the...Objective： The Chinese Quality of Life Instrument （ChQOL） was developed as a valid generic health status instrument based on the well-established theory of health in Chinese medicine. Psychometric properties of the ChQOL were good. In the present study, the responsiveness of the ChQOL in patients with congestive heart failure （CHF） were investigated and compared with two generic questionnaires, the Medical Outcomes Study Short Form 36-item Health Survey （SF-36） and World Health Organization Quality of Life Assessment （WHOQOL-BREF）, as well as one disease-specific questionnaire, the Minnesota Living with Heart Failure Questionnaire （MLHF）. Methods： Thirty-nine in-patients with CHF who had undergone treatment with integrative medicine were recruited. The health status measurements were performed at enrolment and after a 4-week treatment. The following responsiveness indices were used： the effect size （ES） and standardized response mean （SRM）. All patients were classified into those groups with stable measures and those groups with changes after a 4-week treatment, based upon both the doctor＇s global rating of changes in heart function tests, and the patient＇s global rating of changes in overall quality of life. Results： All domains of the ChQOL showed significant improvement. In the comparison of the responsiveness indices, the ChQOL was regarded as more responsive than the WHOQOL-BREF or SF-36 utility, but it was less responsive than the MLHF. Conclusions： The ChQOL was better in sensitivity and responsiveness for assessing congestive heart failure as a generic measure than the SF-36 and WHOQOL-BREF. The ChQOL is considered suitable as an outcome measure for clinical trials in patients with congestive heart failure.展开更多
Objective： The reporting of patient-reported outcomes （PRO） instrument development is vital for both researchers and clinicians to determine its validity, thus, we propose the Preferred Reporting Items for PRO Inst...Objective： The reporting of patient-reported outcomes （PRO） instrument development is vital for both researchers and clinicians to determine its validity, thus, we propose the Preferred Reporting Items for PRO Instrument Development （PRIPROID） to improve the quality of reports. Methods： Abiding by the guidance published by the Enhancing the QUAlity and Transparency Of health Research （EQUATOR） Network, we had performed 6 steps for items development： identified the need for a guideline, performed a literature review, obtained funding for the guideline initiative, identified participants, conducted a Delphi exercise and generated a list of PRIPROID items for consideration at the face-to-face meeting. Results： Twenty three items subheadings under 7 topics were included： title and structured abstract, rationale, objectives, intention, eligibility criteria, conceptual framework, items generation, response options, scoring, times, administrative modes,burden assessment, properties assessment, statistical methods, participants, main results, and additional analysis, summary of evidence, limitations, clinical attentions, and conclusions, item pools or final form, and funding. Conclusions： The PRIPROID contains many elements of the PRO research, and this assists researchers to report their results more accurately and to a certain degree use this instrument to evaluate the quality of the research methods.展开更多
Nanozymes are nanomaterials exhibiting intrinsic enzymatic properties,which suggests that inorganic nanoparticles could be bioactive.It’s in 2007,when we introduced the biological methodologies for natural enzymes to...Nanozymes are nanomaterials exhibiting intrinsic enzymatic properties,which suggests that inorganic nanoparticles could be bioactive.It’s in 2007,when we introduced the biological methodologies for natural enzymes to systematically study the enzymatic properties of iron oxide nanoparticles,we discovered nanozymes.We found that nanozymes,just as natural enzymes,are able to catalyze biochemical reactions under the physiological conditions,following the similar enzymatic kinetics and catalytic mechanisms(Gao et al.,2007).Since then,the developments of nanozymes bloomed,and lots of nanomaterials were found exhibiting various enzymatic activities(Huang et ah,2019).展开更多
基金Chongqing Sports Scientific Research Project“Research and Development of Balance Rhythm Dance and Its Application in Reducing Fall Risk in Older Women”(Project No.D202209)Chongqing Nursing Vocational College College-level Project“Research on the Talent Training Model of Field Engineers in the Intelligent Health Care Sector Based on Rehabilitation Assistive Devices under the‘Integration of Science and Education’Approach”(Project No.Y202307)+1 种基金Chongqing Science and Technology Bureau Research Project“Investigating the Impact of Electro-acupuncture Applied to Antagonist Muscles on Walking Ability in Stroke Patients with Hemiplegia Based on the Principle of Reciprocal Inhibition”(Project No.CSTC2019JXJL130019)Chongqing Traditional Chinese Medicine Hospital Research Project“The Alterations in the Pelvic Floor Muscle Group Following Acupuncture Treatment for Postpartum Stress Urinary Incontinence Were Evaluated Using SWE Technology”(Project No.jxyn2021-2-23)。
文摘Objective: To investigate the effect of a self-developed balance rhythm dance program on the rehabilitation of motor function and the reduction of fall risk in elderly women with diminished balance function. Methods: Fifty elderly women with reduced balance function, admitted to the Qingbar Elderly Care Center of Chongqing Medical University from December 2022 to December 2023, were randomly selected and divided into two groups. The aerobic exercise group (25 patients) received traditional treatment and rehabilitation nursing, while the balance rhythm dance intervention group (25 patients) received the balance rhythm dance intervention in addition to traditional treatment and rehabilitation nursing. The Unified Parkinson’s Disease Rating Scale (UPDRS) and Berg Balance Scale (BBS) were used as evaluation indicators to compare the intervention effects between the two groups. Results: The data revealed that the balance rhythm dance intervention significantly improved the motor ability and balance function of elderly women in the intervention group (P < 0.01), with statistically significant differences observed. Conclusion: The balance rhythm dance program plays a critical role in promoting the rehabilitation of motor function and balance ability in elderly women, effectively enhancing their quality of life.
基金supported by Chinese Academy of Sciences MRI Technology Alliance under Grant 2020GZ1003.
文摘Proton nuclear(^(1)H)is the observed nucleus on which most magnetic resonance imaging(MRI)applications depend.Most traditional^(1)H MRI can provide structural and functional information about organisms,while various non-proton nuclei(X-nuclei)MRI can provide more metabolic information.However,due to the relatively poor signal-to-noise ratio(SNR)of X-nuclei MRI,their applications are quite rare compared to^(1)H.Benefit from the rapid developments of MRI hardware and software technologies,X-nuclei MRI has recently attracted increasing interests in biomedical research.This review firstly introduces some current methods to improve the SNR of X-nuclei MRI.Secondly,this review describes biomedical applications of X-nuclei MRI,especially focusing on the current use of X-nuclei(^(13)C,^(17)O,^(19)F,^(23)Na and^(31)P)MRI to study related diseases in different organs,including the brain,liver,kidney,heart and bone.Finally,perspectives studies on X-nuclei imaging and its potential applications are described in biomedical research.
基金This work was partly supported by the Cancer Prevention and Research Institute of Texas,USA(PR150551 and RP190561)the Welch Foundation(AU-0042-20030616)+1 种基金The work was also supported by the National Natural Science Foundation of China(31700778 and 31320103918)Jiangsu Province’s Key Laboratory of Medicine(XK201135).
文摘In analyses of protein families that may serve as drug targets,membrane-associated G-protein-coupled receptors(GPCRs)dominate,followed by ion channels,transporters,and—to a lesser extent—membrane-bound enzymes.However,various challenges put such membrane proteins among key groups of underutilized opportunities for the application of therapeutic antibodies.Antibodies hold the promise of exquisite specificity,as they are able to target even specific conformations of a particular membrane protein,as well as adaptability through engineering into various antibody formats.However,the ease of raising and isolating specific,effective antibodies targeting membrane proteins depends on many factors.In particular,the generation of specific antibodies is easier when targeting larger,simpler,extracellular domains with greater uniqueness of amino acid sequence.The rareness of such ideal conditions is illustrated by the limited number of approved biologics for targeting GPCRs and other complex membrane proteins.Challenges in developing antibodies to complex membrane proteins such as GPCRs,ion channels,transporters,and membrane-bound enzymes can be addressed by the design of the antigen,antibody-generation strategies,lead optimization technologies,and antibody modalities.A better understanding of the membrane proteins being targeted would facilitate mechanism-based drug discovery.This review describes the advantages and challenges of targeting complex membrane proteins with antibodies and discusses the preparation of membrane protein antigens and antibody generation,illustrated by select examples of success.
基金the National Natural Science Foundation of China(No,30371718)
文摘Objective： The Chinese Quality of Life Instrument （ChQOL） was developed as a valid generic health status instrument based on the well-established theory of health in Chinese medicine. Psychometric properties of the ChQOL were good. In the present study, the responsiveness of the ChQOL in patients with congestive heart failure （CHF） were investigated and compared with two generic questionnaires, the Medical Outcomes Study Short Form 36-item Health Survey （SF-36） and World Health Organization Quality of Life Assessment （WHOQOL-BREF）, as well as one disease-specific questionnaire, the Minnesota Living with Heart Failure Questionnaire （MLHF）. Methods： Thirty-nine in-patients with CHF who had undergone treatment with integrative medicine were recruited. The health status measurements were performed at enrolment and after a 4-week treatment. The following responsiveness indices were used： the effect size （ES） and standardized response mean （SRM）. All patients were classified into those groups with stable measures and those groups with changes after a 4-week treatment, based upon both the doctor＇s global rating of changes in heart function tests, and the patient＇s global rating of changes in overall quality of life. Results： All domains of the ChQOL showed significant improvement. In the comparison of the responsiveness indices, the ChQOL was regarded as more responsive than the WHOQOL-BREF or SF-36 utility, but it was less responsive than the MLHF. Conclusions： The ChQOL was better in sensitivity and responsiveness for assessing congestive heart failure as a generic measure than the SF-36 and WHOQOL-BREF. The ChQOL is considered suitable as an outcome measure for clinical trials in patients with congestive heart failure.
基金Supported by the National Natural Science Foundation of China(No.81073163)Eleventh Five-Year National Key Technology Research and Development Program(No.2006BA104A12)
文摘Objective： The reporting of patient-reported outcomes （PRO） instrument development is vital for both researchers and clinicians to determine its validity, thus, we propose the Preferred Reporting Items for PRO Instrument Development （PRIPROID） to improve the quality of reports. Methods： Abiding by the guidance published by the Enhancing the QUAlity and Transparency Of health Research （EQUATOR） Network, we had performed 6 steps for items development： identified the need for a guideline, performed a literature review, obtained funding for the guideline initiative, identified participants, conducted a Delphi exercise and generated a list of PRIPROID items for consideration at the face-to-face meeting. Results： Twenty three items subheadings under 7 topics were included： title and structured abstract, rationale, objectives, intention, eligibility criteria, conceptual framework, items generation, response options, scoring, times, administrative modes,burden assessment, properties assessment, statistical methods, participants, main results, and additional analysis, summary of evidence, limitations, clinical attentions, and conclusions, item pools or final form, and funding. Conclusions： The PRIPROID contains many elements of the PRO research, and this assists researchers to report their results more accurately and to a certain degree use this instrument to evaluate the quality of the research methods.
基金supported by Sanming Project of Medicine in Shenzhen(SZSM201612031)Chinese Academy of Sciences(YJKYYQ20180048)+5 种基金the National Natural Science Foundation of China(81722024,81772736 and 81571728)the Basic Research Foundation for Shenzhen s Science and Technology(20190726095103499)Project of CAS Laboratory for Nanozyme(KFJ-PTXM-013)the National Key Research and Development Program of China(2017YFA0205501,2017YFA0205503)the Key Research Program of Frontier Sciences of Chinese Academy of Sciences(QYZDY-SSW-SMC013)the Youth Innovation Promotion Association(2014078).
文摘Nanozymes are nanomaterials exhibiting intrinsic enzymatic properties,which suggests that inorganic nanoparticles could be bioactive.It’s in 2007,when we introduced the biological methodologies for natural enzymes to systematically study the enzymatic properties of iron oxide nanoparticles,we discovered nanozymes.We found that nanozymes,just as natural enzymes,are able to catalyze biochemical reactions under the physiological conditions,following the similar enzymatic kinetics and catalytic mechanisms(Gao et al.,2007).Since then,the developments of nanozymes bloomed,and lots of nanomaterials were found exhibiting various enzymatic activities(Huang et ah,2019).
