Among the crop production factors,preceding crop and tillage management affect the sustainable use of soil resources and ultimately crop growth and productivity.This study aimed at investigating the impact of precedin...Among the crop production factors,preceding crop and tillage management affect the sustainable use of soil resources and ultimately crop growth and productivity.This study aimed at investigating the impact of preceding winter crops(grass or legume)and different tillage systems on forage yield,quality and nutritive values of three summer grass(Sudan grass,pearl millet and teosinte)and two legume forage crops(cowpea and guar)under arid conditions.The results exhibited that growing forage crops after legumes(as berseem clover)produced the highest fresh and dry forage yields and quality attributes compared with grasses(as wheat)with the exception of crude fiber content,which was decreased.Moreover,tillage practices showed positive impact on forage yields and quality attributes.The maximum forage yields and quality parameters were recorded under conventional tillage(CT)practice compared with reduced tillage(RT)and no-tillage(NT)systems.Among the evaluated crops,the highest yields of fresh forage,dry forage,crude fiber,crude protein and total digestible nutrient were exhibited by grass forage crops(Sudan grass,pearl millet and teosinte),whereas the highest crude protein content and the digestible energy values were produced by legume forage crops(cowpea and guar).The maximum fresh forage,dry forage,crude fiber,crude protein,total digestible nutrient and digestible crude protein yields were produced by pearl millet followed by Sudan grass under CT and RT after berseem clover.The highest net return was recorded by sowing pearl millet after berseem clover and applying CT followed by RT practices,which could be recommended for the commercial production.Moreover,it could be assumed that the combination of growing grass forage crops after legume crops under CT or RT systems could enhance forage crop yield and quality with an improvement in soil properties for sustainable agriculture with low cost and the highest net income.展开更多
A two-year study was carried out in Giza Agricultural Experiments and Research Station, Agricultural Research Center (ARC), Giza, Egypt in the 2020 and 2021 summer seasons to evaluate the relative tolerance of ten soy...A two-year study was carried out in Giza Agricultural Experiments and Research Station, Agricultural Research Center (ARC), Giza, Egypt in the 2020 and 2021 summer seasons to evaluate the relative tolerance of ten soybean genotypes for cotton leafworm infestation under field conditions. Soybean genotypes H<sub>11</sub>L<sub>145</sub>, H<sub>155</sub>, H<sub>113</sub>, H<sub>4</sub>L<sub>4</sub>, H<sub>15</sub>L<sub>17</sub>, H<sub>129</sub>, H<sub>30</sub>, H<sub>19</sub>L<sub>96</sub>, Giza 111, and Crawford were distributed in randomized complete blocks design with three replications. Soybean genotypes differed significantly for cotton leafworm at the 6<sup>th</sup>, 7<sup>th</sup>, and 8<sup>th</sup> week from sowing. Low values of cotton leafworm assemblages were recorded for H<sub>113</sub>, H<sub>4</sub>L<sub>4</sub>, H<sub>15</sub>L<sub>17</sub>, Giza 111, and H<sub>129</sub>. Low percentages of the larval survival number and weight, as well as the number of the survival of pupa were recorded by feeding on leaves of genotypes H<sub>4</sub>F<sub>4</sub>, H<sub>15</sub>L<sub>17</sub>, and Giza 111 under laboratory conditions. There were significant differences among the studied genotypes in most yield attributes in both seasons. Soybean genotypes H<sub>15</sub>L<sub>17</sub>, Giza 111, H<sub>113</sub>, H<sub>129</sub>, H<sub>19</sub>L<sub>96</sub>, and H<sub>4</sub>L<sub>4 </sub>gave higher seed yield per ha, meanwhile soybean genotypes H<sub>155</sub>, H<sub>19</sub>L<sub>96</sub>, H<sub>30</sub>, Giza 111, and H<sub>15</sub>L<sub>17</sub> had higher seed oil content than the other genotypes in both seasons. The number and weight of larvae surviving, as well as the number of pupa survival, were negatively correlated with leaf total phenols and seed oil content. It can be concluded that soybean genotypes H<sub>15</sub>L<sub>17</sub>, H<sub>4</sub>L<sub>4</sub>, and Giza 111 are promising genotypes with desirable seed oil content for tolerating cotton leafworm infestation in breeding programs.展开更多
Direct-seeding rapeseed production at high plant density raises the risk of lodging.We investigated the use of dwarf genes to improve rapeseed plant architecture to balance yield and lodging.Three genotypes with diffe...Direct-seeding rapeseed production at high plant density raises the risk of lodging.We investigated the use of dwarf genes to improve rapeseed plant architecture to balance yield and lodging.Three genotypes with different plant architectures(dwarf sca^(HS5),semi-dwarf+/sca^(HS5),and tall ^(HS5))were evaluated under varying nitrogen rates(N1,N2,and N3:120,240,and 360 kg N ha^(-1))and plant densities(D1,D2,and D3:15,45,and 75 plants m^(-2))from 2019 to 2022.The results showed that increasing N rate positively influenced yield while decreasing lodging resistance in all genotypes.Increasing plant density(D2-D3)enhanced lodging resistance and yield in sca^(HS5) and+/sca^(HS5),but reduced yield in ^(HS5).Compared to the two parents,+/sca^(HS5) exhibited moderate expressions of IAA3,GH3.15,and SAUR30 in stems under N2D3,resulting in reduced plant height and increased compactness.Additionally,+/sca^(HS5) had a thicker silique layer than ^(HS5) by 14.7%,and it had a significant correlation between branch height/angle and yield.Increasing N rate led to increased lignin and pectin contents,while cellulose content decreased.Increasing plant density resulted in greater stem cellulose content and CSLA3/7 expression in sca^(HS5) and+/sca^(HS5),but decreased in ^(HS5).Compared to ^(HS5),+/sca^(HS5) exhibited higher expressions of ARAD1 and GAUT4,along with a 51.1%increase in pectin content,leading to improved lodging resistance under N2D3.Consequently,+/sca^(HS5) showed a 46.4%higher yield and 38.9%lodging resistance than ^(HS5) under N2D3,while sca^(HS5) demonstrated strong lodging resistance but lower yield potential.Overall,this study underscores the potential of utilizing auxin dwarf genes to optimize the trade-off between yield and lodging resistance in rapeseed and the possibility of maximizing yield potential by optimizing the plant architecture of+/sca^(HS5) through nitrogen reduction and dense planting.展开更多
In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain yield.Melatonin deficiency led to transcriptional reprogr...In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain yield.Melatonin deficiency led to transcriptional reprogramming,especially of genes involved in chlorophyll and carbon metabolism,redox regulation,and transcriptional regulation,during dark-induced leaf senescence.Hypomethylation of mCG and mCHG in the melatonin-deficient rice mutants was associated with the expression change of both protein-coding genes and transposable element-related genes.Changes in gene expression and DNA methylation in the melatonin-deficient mutants were compensated by exogenous application of melatonin.A decreased S-adenosyl-L-methionine level may have contributed to the DNA methylation variations in rice mutants of melatonin deficiency under dark conditions.展开更多
Rice(Oryza sativa L.)is one of the most important cereal crops in the world.Bakanae disease is a significant rice disease widely distributed in rice-growing regions worldwide.Therefore,the present investigation aimed ...Rice(Oryza sativa L.)is one of the most important cereal crops in the world.Bakanae disease is a significant rice disease widely distributed in rice-growing regions worldwide.Therefore,the present investigation aimed to assess the optimal concentrations of paclobutrazol(PBZ)as a treatment for rice grains(cv.Sakha 108)to control bakanae disease,also evaluating its impact on grain germination,seedling growth parameters as well as disease index.Paclobutrazol concentrations had no significant impact on seed germination,regardless of whether the seeds were incubated with Fusarium fujikuroi or not.Application of PBZ,either alone or in combination with fungal pathogens,negatively impacted the rice seedlings’height.Paclobutrazol at 25,50 and 100 mg/L,combined with the fungal pathogen positively impacted root length.Paclobutrazol at 3 and 6 mg/L mitigated the adverse impact on chlorophyll pigments content in infected seedlings.The highest proline contents were achieved by 100 mg/L PBZ alone or in combination with fungal pathogens.It has been observed that the application of PBZ,either alone or in combination with a fungal pathogen,leads to the enhancement of catalase,peroxidase,and polyphenol oxidase activities.The median lethal concentration of PBZ was 0.874 mg/L;applying low concentrations of paclobutrazol effectively increased the percentage of fungal growth suppression.Application of PBZ,at higher concentrations(50 and 100 mg/L),decreased infection percentage and disease severity index(DSI)significantly.These findings suggest that PBZ can be an effective treatment for controlling bakanae disease and enhancing resistance in rice plants.展开更多
Twenty-four rice genotypes were examined to assess genetic variability,heritability,and correlations for seven-grain quality traits,eight nutritional elements,and protein.ANOVA revealed significant differences for the ...Twenty-four rice genotypes were examined to assess genetic variability,heritability,and correlations for seven-grain quality traits,eight nutritional elements,and protein.ANOVA revealed significant differences for the quality traits studied.For every trait under study,the phenotypic coefficient of variation was higher than the correspon-dence genotypic coefficient of variation.Heritability in a broad sense varied from 29.75%for grain length to 98.31%for the elongation trait.Hulling percentage recovery had a highly significant positive correlation with milling and head rice percentage.Consequently,milling percentage had a highly positive correlation with head rice percentage.In amylose percentage,all the genotypes belonged to low amylose except the Hassawi-1 variety,which had intermediate amylose content.Mineral nutrition contents of magnesium(Mg),sodium(Na),potas-sium(K),calcium(Ca),copper(Cu),manganese(Mn),zinc(Zn),iron(Fe),or protein percentage gave different variations for 24 rice genotypes under all the nutritional elements.Among the 24 genotypes,ten rice genotypes–HighNutrient-1,HighNutrient-2,HighNutrient-9,HighNutrient-8,HighNutrient-3,Hassawi-2,HighNutrient-7,HighNutrient-6,Hassawi-1,and HighNutrient-4–had the highest heist value for all nutritional and protein con-tents,and could be used as a donor to improving new varieties.There was a positive and significant correlation between magnesium Mg,K,Zn and Fe.Consequently,K had a positive correlation with zinc Zn,Fe,and protein percentage.Clustering analysis was divided into two groups:thefirst group included all genotypes rich in nutri-ents,while the remaining genotypes with low nutritional content were included in the second group.展开更多
Barley production has been constrained by various factors,of which loose smut(Ustilago nuda)is the main biotic factor.Molecular and agronomical screening analyses were evaluated to study the similarity levels and mark...Barley production has been constrained by various factors,of which loose smut(Ustilago nuda)is the main biotic factor.Molecular and agronomical screening analyses were evaluated to study the similarity levels and marker assisted selection associated with resistance to loose smut among eight barley genotypes using sequence-related amplified polymorphism(SRAP).Agronomical parameters during two consecutive 2021 and 2022 seasons were studied to identify their reaction to loose smut.The results exhibited significant differences among all studied genotypes for all studied characters,and the highest mean values for all studied traits were detected in Giza 136 and Giza 137.Six SRAP selected primer combinations were amplified and gave 56 total fragments,where primer combination me1+em3 gave the highest polymorphism(100%)and the highest polymorphic information content of PIC was 0.96.The dendrogram of SRAP markers had clustered all studied genotypes into two main clusters.Cluster I includes all the resistance genotypes Giza 136,Giza 137,Giza 123,Giza 132,Giza 138 and Line 2.However,cluster II includes only Line 1 and Line 3 as susceptible genotypes.Thus,SRAP marker could be efficiently used to assess genetic variation among barley genotypes and useful for barley germplasm management in terms of biodiversity protection and design of new crosses for loose smut breeding programs,and seed dressings are commonly used to prevent infected seed from developing smutted heads.Some effective seed dressings include Triticonazole,Black seed oil and gamma rays 150,200,250 Gy.The tested fungicide was highly effective in controlling the disease and gave more than 99%disease control with high grain yield while nature product as black seed oil and utilizing radiation gamma ray 250 GY was the most effective.Utilizing radiation and natural ingredients to manage loose barley smut is economical.展开更多
Monogenic lines,which carried 23 genes for blast resistance were tested and used donors to transfer resistance genes by crossing method.The results under blast nursery revealed that 9 genes from 23 genes were suscepti...Monogenic lines,which carried 23 genes for blast resistance were tested and used donors to transfer resistance genes by crossing method.The results under blast nursery revealed that 9 genes from 23 genes were susceptible to highly susceptible under the three locations(Sakha,Gemmeza,and Zarzoura in Egypt);Pia,Pik,Pik-p,Piz-t,Pita,Pi b,Pi,Pi 19 and Pi 20.While,the genes Pii,Pik-s,Pik-h,Pi z,Piz-5,Pi sh,Pi 3,Pi 1,Pi 5,Pi 7,Pi 9,Pi 12,Pikm and Pita-2 were highly resistant at the same locations.Clustering analysis confirmed the results,which divided into two groups;the first one included all the susceptible genes,while the second one included the resistance genes.In the greenhouse test,the reaction pattern of five races produced 100%resistance under artificial inoculation with eight genes showing complete resistance to all isolates.The completely resistant genes:Pii,Pik-s,Piz,Piz-5(=bi2)(t),Pita(=Pi4)(t),Pita,Pi b and Pi1 as well as clustering analysis confirmed the results.In the F1 crosses,the results showed all the 25 crosses were resistant for leaf blast disease under field conditions.While,the results in F2 population showed seven crosses with segregation ratio of 15(R):1(S),two cross gave segregated ratio of 3 R:1 S and one gave 13:3.For the identification of blast resistance genes in the parental lines,the marker K3959,linked to Pik-s gene and the variety IRBLKS-F5 carry this gene,which was from the monogenic line.The results showed that four genotypes;Sakha 105,Sakha 103,Sakha 106 and IRBLKS-F5 were carrying Pik-s gene,while was absent in the Sakha 101,Sakha 104,IRBL5-M,IRBL9-W,IRBLTACP1 and IRBL9-W(R)genotypes.As for Pi 5 gene,the results showed that it was present in Sakha 103 and Sakha 104 varieties and absent in the rest of the genotypes.In addition,Pita-Pita-2 gene was found in the three Egyptian genotypes(Sakha 105,Sakha 101 and Sakha 104)plus IRBLTACP1 monogenetic.In F2 generation,six populations were used to study the inheritance of blast resistance and specific primers to confirm the ratio and identify the resistance genes.However,the ratios in molecular markers were the same of the ratio under field evaluation in the most population studies.These findings would facilitate in breeding programs for gene pyramiding and gene accumulation to produce durable resistance for blast using those genotypes.展开更多
Rice(Oryza sativa L.)is an important cereal crop,consumed as a staple by more than 50%of the global population(Gathala et al,2011).In Asian countries,the rice production increased to 695.5 million tones in the last ye...Rice(Oryza sativa L.)is an important cereal crop,consumed as a staple by more than 50%of the global population(Gathala et al,2011).In Asian countries,the rice production increased to 695.5 million tones in the last years,and this increasing was caused by the main source of income and employment in these countries.Breeding high-yielding hybrid rice is a potentially promising solution for addressing food shortages caused by marked increases in the global population.Recent progress in DNA marker technology and the construction of linkage maps has enabled the detection of quantitative trait loci(QTLs)that regulate different agronomic traits applicable to breeding(Parida et al,2006;Zhang et al,2017).展开更多
Wheat was the first crop grown in Egypt, and it remains highly important. Egypt is the largest wheat importer in the world and consumes an extensive amount of bread. It is imperative for wheat scientists to decrease t...Wheat was the first crop grown in Egypt, and it remains highly important. Egypt is the largest wheat importer in the world and consumes an extensive amount of bread. It is imperative for wheat scientists to decrease the large gap between production and consumption. Wheat yields in Egypt increased 5.8-fold(6.7 billion kg) between 1961 and 2017 due to variety improvement and the use of better planting methods such as the raised bed method, ideal sowing date, surge flow irrigation and farm irrigation systems, laser levelling, fertilizers, and intercropping with raised beds. In this paper, the development of wheat production techniques and variety evolution over more than five decades in Egypt have been analyzed. In particular, we have focused on the technologies, cultural practices and causes for per unit area yield increase. The main purpose was to study the issues that have arisen during wheat production and to make recommendations for smart agricultural practices. In 1981, the yield was 3 300 kg ha–1 and through the improvement of varieties, expansion of agricultural land and the adoption of modern agricultural techniques yield reached 6 500 kg ha–1 by 2017. The production growth rate was 4.1% annually, and the total grain yield increased 4.3-fold, from 1.9 billion kg in 1981 to about 8.1 billion kg in 2017. The use of new improved varieties, new cultivation techniques, and modern irrigation techniques contributed to 97.0% of the increase in yield per unit area and 1.5% of the increase in yield was due to planting area expansion. Therefore, the increase in total yield mainly depended on the increase in yield per unit area. Wheat production in Egypt has been improved through the development of breeding and cultivation techniques. The use of these new techniques, the popularization of new high-quality seed varieties, and the use of the raised bed method instead of the old method of planting in basins have made the largest contributions to increased yield. In the future, wheat yield could be further increased by using the tridimensional uniform sowing mode and the development of wheat varieties that are resistant to rusts, deficit irrigation, and abiotic stress, that are highly adaptable to mechanized operation and have high yields. Based on our analysis, we propose the main technical requirements and measures to increase wheat yield in Egypt in the near future.展开更多
Adjustment of planting geometry along with suitable maize cultivar can be a viable tool for maximizing land usage and net return. A two-year study was carried out at Serw Agricultural Experiments and Research Station,...Adjustment of planting geometry along with suitable maize cultivar can be a viable tool for maximizing land usage and net return. A two-year study was carried out at Serw Agricultural Experiments and Research Station, A.R.C., Domiate governorate, Egypt, during 2015 and 2016 seasons to evaluate intercropping cowpea with suitable maize cultivar and its planting geometry for maximizing land usage and net return. The treatments were the combinations between three maize cultivars (SC 30K08, TWC 310 and TWC 352) and three maize plant distributions (one plant/hill distanced at 25, 50 and 75 cm between hills, respectively). Ridge width with maize plant distribution formed maize planting geometry (25 cm × 140 cm, 50 cm × 140 cm and 75 cm × 140 cm). These treatments were compared in a split plot distribution in randomized complete block design with three replications. Maize cultivar SC 30K08 had the highest grain yield and its attributes compared with the other cultivars in both seasons. Also, maize planting geometry 25 cm × 140 cm resulted in the highest grain yield and its attributes compared with the other planting geometries in both seasons. Ear length, ear weight, 100-grain weight and grain yield/fad were affected significantly by maize cultivars × maize planting geometry. Intercropping cowpea with maize cultivar TWC 352 had the highest seed yield and its attributes compared with those intercropped with the other cultivars in both seasons. Also, maize planting geometry 75 cm × 140 cm resulted in the highest seed yield and its attributes through growing two cowpea rows between maize hills compared with the other maize planting geometries in both seasons. Number of pods/plant and seed yield/fad were affected significantly by maize cultivars × maize planting geometry. LER and LEC values of the intercrops were much greater than 1.00 and 0.25, respectively, for all the combinations indicating less land requirements of intercropping system than solid culture of both crops. Growing one row of maize cultivar TWC352 in both sides of bed 140 cm width with cowpea two rows in middle of the bed increased land productivity and net return compared with solid culture of maize.展开更多
Seed priming has proved to be an effective pre-germination seed invigoration technique for different crops to improve seed and seedling performance under different abiotic stresses.In Bangladesh,winter rice is very of...Seed priming has proved to be an effective pre-germination seed invigoration technique for different crops to improve seed and seedling performance under different abiotic stresses.In Bangladesh,winter rice is very often exposed to cold waves just after sowing in the nursery bed resulting in poor seed germination and seedling emergence,yellowish and thin seedlings production,and a very low survival rate.Seed priming may mitigate the cold stress during seed germination and seedling emergence and helps in the quality seedling production of winter rice.To evaluate the efficacy of different seed priming techniques in increasing seedling emergence,growth,vigor and survivability of winter rice cultivars under cold stress,a pot experiment was conducted at the Department of Agronomy,Bangladesh Agricultural University during December 2018 to January 2019.The experiment comprised two factors,(A)Winter rice variety namely,(i)BRRI dhan29 and (ii)BRRI dhan36;(B)Seed priming agent namely (i)Control(no priming),(ii)20000 ppm NaCl,(iii)30000 ppm NaCl,(iv)20000 ppm KCl,(v)30000 ppm KCl,(vi)20000 ppm CaCl2,(vii)30000 ppm CaCl2,(viii)50 ppm CuSO4,(ix)75 ppm CuSO_(4),(x)10000 ppm ZnSO4,(xi)15000 ppm ZnSO4,(xii)2 ppm Na2MoO4,(xiii)3 ppm Na2MoO4,(xiv)100 ppm PEG(Polyethylene glycol 4000)and (xv)150 ppm PEG.Seeds were sown on two different dates viz.,1st December and 1st January so that seedlings are exposed to cold stress at different stages.The experiment was laid out in a completely randomized design(CRD)with three replications.Results indicated that(in most of the cases)seed priming has a positive impact on seedling emergence rate(%),root length,shoot length,root shoot ratio,root dry weight,shoot dry weight,seedling dry weight and survival rate(%).Among the priming agents,KCl and CaCl2 performed best;while priming with NaCl and PEG showed no advantages over no priming for both the sowing dates.In general,BRRI dhan36 performed better than BRRI dhan29 in terms of seedling growth because of its higher tolerance to cold stress.But,both the varieties performed similarly in terms of emergence rate and survival rate.Thus,priming is an effective tool to increase seed germination,better seedling growth,and higher seedling survivability of winter rice under cold stress,and KCl(20000 ppm)or CaCl2(20000 ppm)can be considered as a viable priming agent.展开更多
The study estimated genetic gain for yield and other traits in winter wheat released for irrigated environments in Turkey from 1963 to 2004. Yield trials including 14 varieties were grown in 16 environments from 2008 ...The study estimated genetic gain for yield and other traits in winter wheat released for irrigated environments in Turkey from 1963 to 2004. Yield trials including 14 varieties were grown in 16 environments from 2008 to 2012 in provinces of Konya, Eski?ehir, Ankara, and Edirne. The highest yields were achieved by recent varieties Kinaci-97(5.48 t ha^(-1)),Cetinel-2000(5.39 t ha^(-1)), Alpu-2001(5.44 t ha^(-1)), Ahmetaga(5.35 t ha^(-1)), and Ekiz-2004(5.42 t ha^(-1)) compared to older varieties Yektay-406(4.17 t ha^(-1)) and Bezostaya-1(4.27 t ha^(-1))released in the 1960 s. The progress reached in grain yield in 20 years was 1.16 t ha^(-1)or58 kg ha^(-1)(1.37%) per year. This gain was mainly achieved through shorter plant height and increased harvest index. There was no clear tendency of changes in specific yield components demonstrating that new high-yielding varieties may have different ways to reach their yield potentials. The yield gains were accompanied by improved stripe rust and leaf rust resistances primarily based on adult plant resistance genes. The grain quality of the new varieties did not deteriorate over time although most of them were inferior to the bread-making quality check Bezostaya-1, a feature that may require attention in future breeding.展开更多
Vegetable production in South East Asia often is in rotation with flooded rice. The puddling of the soil with flooded rice production may result in unfavourable soil conditions for the subsequent production of dry lan...Vegetable production in South East Asia often is in rotation with flooded rice. The puddling of the soil with flooded rice production may result in unfavourable soil conditions for the subsequent production of dry land crops. To establish whether permanent vegetable production results in favourable soil conditions for vegetables, the effects of five different permanent vegetable production systems and a system of vegetable production in rotation with flooded rice on soil properties after flooded rice were studied in a 2-year field experiment. Bulk density at 0.05–0.10 m depth layer decreased with permanent vegetable production and vegetable production in rotation with flooded rice. The decrease in bulk density was influenced by the application of organic manure and rice husks, and especially by the number of crops cultivated, suggesting that frequency of soil tillage had a major effect on bulk density. Ploughing with buffalo traction after flooded rice, in combination with construction of raised beds, could reduce or totally eliminate negative effects of puddling on soil structure. Bulk density at 0.15–0.20 m soil depth was not influenced. Soil acidity decreased significantly in all systems. Soil organic carbon increased in all systems, but significant increase was only found in two permanent vegetable production systems. Available phosphorus(P) significantly increased in two permanent vegetable production systems, with a positively correlation to the amount of P applied. The significant decrease in bulk density and increase in p H(H2O), after only 2 years, showed that soil conditions after flooded rice could be improved in a short time under intensive vegetable production.展开更多
Ten Egyptian barley genotypes (2 commercial varieties and 8 breeding lines) were cultivated under normal condition at the Experimental Farm of Sakha Agricultural Research station and exposed to salinity stress conditi...Ten Egyptian barley genotypes (2 commercial varieties and 8 breeding lines) were cultivated under normal condition at the Experimental Farm of Sakha Agricultural Research station and exposed to salinity stress condition at the Experimental Farm of El-hosainia plain Agricultural Research station, Elsharkia Governorate, Egypt, in an attempt to identify the relative salinity tolerant genotypes. A susceptibility index (SI) was used to estimate the relative stress loss because it accounted for variation in yield potential and stress intensity. Giza 123, Line-1, Line-5, Line-6 and Line-8 genotypes were considered as saline tolerant genotypes on the basis of their highly tolerance indices values. Barley genotypes were characterized by seven SSR markers and three SCoT primers in different combinations to discern the extent of genetic variation and develop a fingerprinting key. Normal SCoT reactions amplify single segments of DNA which are 15- to 19-mer long. A new strategy was used to increase SCoT potential in genetic diversity studies by using two and three different primer combinations per reaction. Amplification products scored a polymorphism percentage of 94.44% for Triple-SCoT and 90.91% for SSR, while the average no. of polymorphic fragments/primer was 17 and 7.14 in the two marker systems, respectively. On the other side, Triple-SCoT exhibited the highest average number of positive and negative genotype-specific markers. The cluster analysis of the studied genotypes using these different marker systems revealed four dendrograms varied in their topology. The dendrogram based on Triple-SCoT data exhibited the closest relationships to those illustrated by SSR dendrogram.展开更多
Water deficit is a limiting factor in sesame cultivars yield,thus identification of genetic mechanisms of sesame traits under water stress conditions is essential to development of water stress tolerant genotypes.Henc...Water deficit is a limiting factor in sesame cultivars yield,thus identification of genetic mechanisms of sesame traits under water stress conditions is essential to development of water stress tolerant genotypes.Hence,the triple test cross(TTC)analysis in F_(2) population of the sesame cross(NA_(76)NA_(54))was used.Since,30 TTC families with their 13 respective parents were evaluated during the summer season 2020 under two irrigation treatments.i.e.,normal irrigation with 5952.38 m^(3) hm^(-2) of applied water,and water stress with 2976.19 m^(3) hm^(-2) of applied water.This was implemented at the experimental field of Kafr El Hamam/Sharkia,Agricultural Research Station,Agricultural Research Center(ARC),Egypt.Additive(D)and dominance(H)gene actions as well as epistatic and its two components of additiveadditive,additivedominance plus dominancedominance were involved in the inheritance of all studied traits under both irrigation treatments and their combined analysis.The degree of dominance(H/D)^(0.5) verified the presence of partial dominance in most cases.The correlation coefficient between sums(additive)and differences(dominance)was non-significant,showing that dominant genes between lines were ambidirectional.The moderately water stress tolerant TTC families as the best selected families were found in families 22,25,10,12,and 15.Therefore,postponing selection in these selected families to later segregating generations for all studied traits would be effective to exploit the positive effects of additiveadditive epistasis.展开更多
The response of rice to salt stress(200 mmol/L NaCl)was investigated at the transcription level in Egyptian varieties Giza 177(salt sensitive variety)and Giza 178(salt tolerant variety).We applied a genome-wide RNA-Se...The response of rice to salt stress(200 mmol/L NaCl)was investigated at the transcription level in Egyptian varieties Giza 177(salt sensitive variety)and Giza 178(salt tolerant variety).We applied a genome-wide RNA-Seq transcriptome study at 21-day-old seedlings of both varieties,exposed or not to salt stress for 24 h.展开更多
Differences in floral morphologies affect pollination behaviour in many flowering plants. In the genus Oryza, several differences in the size of floral organs are known. In this study, we focused on the differences in...Differences in floral morphologies affect pollination behaviour in many flowering plants. In the genus Oryza, several differences in the size of floral organs are known. In this study, we focused on the differences in the size of floral organs between common cultivated rice, Oryza sativa L. and its wild ancestor, O. rufipogon. We compared floral morphologies between cultivated rice O. sativa cv. Nipponbare and O. rufipogon W630. We first evaluated temporal changes in filament and anther lengths. W630 had longer filaments with rapid elongation within 15 min after spikelet opening. W630 also had longer anthers than Nipponbare, and size of anther was consistent throughout all time examined. We also analysed other six floral traits, and found that W630 had higher stigma and style length, as well as lemma and palea length, but lower lemma and palea width. Quantitative trait locus (QTL) analysis was performed to identify the loci controlling these floral traits, using backcross recombinant inbred lines derived from a cross between Nipponbare and W630. A total of 11 significant QTLs were identified. Of these, two pairs of QTLs for lemma and palea length and one pair for lemma and palea width overlapped, suggesting that common genetic factors may be the reason for the differences in these traits. In addition, we performed QTL analysis for grain size, and found that QTLs for grain size coincided with those for lemma and palea size, indicating that grain size is partly controlled by glume capacity. The QTLs identified in this study will be informative for understanding genetic changes associated with rice domestication.展开更多
The author regrets that the affiliated institution of Ibthal S.ElDemardash should be“Department of Genetics and Cytology,National Research Centre,Cairo,Egypt”instead of“Department of Genetics and Cytology,National ...The author regrets that the affiliated institution of Ibthal S.ElDemardash should be“Department of Genetics and Cytology,National Research Centre,Cairo,Egypt”instead of“Department of Genetics and Cytology,National Research Center,Cairo,Egypt”.The author would like to apologize for any inconvenience caused.展开更多
A two-year study was carried out at the Agricultural Experiments and Research Station, Faculty of Agriculture, Cairo University, Giza, Egypt, during 2015/2016 and 2016/2017 seasons to evaluate seed yield and quality o...A two-year study was carried out at the Agricultural Experiments and Research Station, Faculty of Agriculture, Cairo University, Giza, Egypt, during 2015/2016 and 2016/2017 seasons to evaluate seed yield and quality of two faba bean cultivars to different rates of amino acids compounds under narrow and wide ridges. Two faba bean cultivars Sakha 1 and Masr 3 were grown in two ridge widths 60 and 120 cm and received five rates of amino acids compounds (Power mix at rates 1.5 cm⋅L−1 “Pm1” and 3.0 cm⋅L−1 “Pm2”, Super mix at rates of 3.5 cm⋅L−1 “Sm1” and 7.0 cm⋅L−1 “Sm2”, as well as, water only “control treatment”). The treatments laid out in a split split plot distribution in randomized complete blocks design in three replications were used. Ridge width was randomly assigned to the main plots, faba bean cultivars were allocated in sub-plots and amino acids compounds were allocated in sub sub-plots. Shoot chemical composition and photosynthesis pigments, as well as, chemical composition in seed traits of faba bean were tested in the laboratories of Plant Physiology Section, Agricultural Botany Department, Faculty of Agriculture, Cairo University, Giza, Egypt. Wide ridge had higher nitrogen (N), phosphorus (P), potassium (K), total sugars and total free amino acids concentrations in faba bean shoot and seeds in addition to chlorophyll a (Chl a) and chlorophyll b (Chl b) in faba bean shoot, as well as, most seed yield attributes than those of the narrow one. Faba bean cultivar Sakha 1 had higher values of seed yield, yield attributes and quality than Masr 3 that achieved higher seed antioxidants. Also, amino acids compounds affected significantly all studied traits of faba bean plants. Pm2 recorded the highest values of chemical composition in faba bean shoot and seeds. Moreover, Pm2 gave the highest seed yield and yield attributes compared to others. The interaction between ridge width and faba bean cultivar was significant for total sugars and carotenoids concentrations in faba bean shoot and 100-seed weight, as well as, seed N content. Also, the interaction between ridge width and amino acids compound had significant effects on shoot and seed N, P, K concentrations, number of pods plant−1 and seed yield ha−1, as well as, total soluble phenols and total free amino acids concentrations. Moreover, the interaction between faba bean cultivar and amino acids compound had significant effects on shoot Chl a and b concentrations, number of seeds pod−1 and seed yield plant−1, as well as, seed N, total soluble phenols and total free amino acids concentrations. Wide ridge x faba bean cultivar x amino acids compound interaction had significant effects on photosynthesis pigments, number of seeds pod−1 and seed yield ha−1, as well as, seed N, total sugars, total soluble phenols and total free amino acids concentrations. High seed yield and quality were recorded by growing faba bean cultivar Sakha 1 in wide ridge with foliar spraying at rate of 3.0 cm⋅L−1 of power mix. On the other hand, growing faba bean cultivar Masr 3 in narrow ridge with foliar spraying at rate of 3.5 cm⋅L−1 of super mix had good antioxidants in faba bean seeds.展开更多
基金the Agricultural Research Center,Giza,Egypt,and the Taif University Researchers Supporting Project(TURSP-2020/111),Taif University,Taif,Saudi Arabia for the technical and financial support to this research。
文摘Among the crop production factors,preceding crop and tillage management affect the sustainable use of soil resources and ultimately crop growth and productivity.This study aimed at investigating the impact of preceding winter crops(grass or legume)and different tillage systems on forage yield,quality and nutritive values of three summer grass(Sudan grass,pearl millet and teosinte)and two legume forage crops(cowpea and guar)under arid conditions.The results exhibited that growing forage crops after legumes(as berseem clover)produced the highest fresh and dry forage yields and quality attributes compared with grasses(as wheat)with the exception of crude fiber content,which was decreased.Moreover,tillage practices showed positive impact on forage yields and quality attributes.The maximum forage yields and quality parameters were recorded under conventional tillage(CT)practice compared with reduced tillage(RT)and no-tillage(NT)systems.Among the evaluated crops,the highest yields of fresh forage,dry forage,crude fiber,crude protein and total digestible nutrient were exhibited by grass forage crops(Sudan grass,pearl millet and teosinte),whereas the highest crude protein content and the digestible energy values were produced by legume forage crops(cowpea and guar).The maximum fresh forage,dry forage,crude fiber,crude protein,total digestible nutrient and digestible crude protein yields were produced by pearl millet followed by Sudan grass under CT and RT after berseem clover.The highest net return was recorded by sowing pearl millet after berseem clover and applying CT followed by RT practices,which could be recommended for the commercial production.Moreover,it could be assumed that the combination of growing grass forage crops after legume crops under CT or RT systems could enhance forage crop yield and quality with an improvement in soil properties for sustainable agriculture with low cost and the highest net income.
文摘A two-year study was carried out in Giza Agricultural Experiments and Research Station, Agricultural Research Center (ARC), Giza, Egypt in the 2020 and 2021 summer seasons to evaluate the relative tolerance of ten soybean genotypes for cotton leafworm infestation under field conditions. Soybean genotypes H<sub>11</sub>L<sub>145</sub>, H<sub>155</sub>, H<sub>113</sub>, H<sub>4</sub>L<sub>4</sub>, H<sub>15</sub>L<sub>17</sub>, H<sub>129</sub>, H<sub>30</sub>, H<sub>19</sub>L<sub>96</sub>, Giza 111, and Crawford were distributed in randomized complete blocks design with three replications. Soybean genotypes differed significantly for cotton leafworm at the 6<sup>th</sup>, 7<sup>th</sup>, and 8<sup>th</sup> week from sowing. Low values of cotton leafworm assemblages were recorded for H<sub>113</sub>, H<sub>4</sub>L<sub>4</sub>, H<sub>15</sub>L<sub>17</sub>, Giza 111, and H<sub>129</sub>. Low percentages of the larval survival number and weight, as well as the number of the survival of pupa were recorded by feeding on leaves of genotypes H<sub>4</sub>F<sub>4</sub>, H<sub>15</sub>L<sub>17</sub>, and Giza 111 under laboratory conditions. There were significant differences among the studied genotypes in most yield attributes in both seasons. Soybean genotypes H<sub>15</sub>L<sub>17</sub>, Giza 111, H<sub>113</sub>, H<sub>129</sub>, H<sub>19</sub>L<sub>96</sub>, and H<sub>4</sub>L<sub>4 </sub>gave higher seed yield per ha, meanwhile soybean genotypes H<sub>155</sub>, H<sub>19</sub>L<sub>96</sub>, H<sub>30</sub>, Giza 111, and H<sub>15</sub>L<sub>17</sub> had higher seed oil content than the other genotypes in both seasons. The number and weight of larvae surviving, as well as the number of pupa survival, were negatively correlated with leaf total phenols and seed oil content. It can be concluded that soybean genotypes H<sub>15</sub>L<sub>17</sub>, H<sub>4</sub>L<sub>4</sub>, and Giza 111 are promising genotypes with desirable seed oil content for tolerating cotton leafworm infestation in breeding programs.
基金supported by the National Key Research and Development Program of China (2021YFD1901200)
文摘Direct-seeding rapeseed production at high plant density raises the risk of lodging.We investigated the use of dwarf genes to improve rapeseed plant architecture to balance yield and lodging.Three genotypes with different plant architectures(dwarf sca^(HS5),semi-dwarf+/sca^(HS5),and tall ^(HS5))were evaluated under varying nitrogen rates(N1,N2,and N3:120,240,and 360 kg N ha^(-1))and plant densities(D1,D2,and D3:15,45,and 75 plants m^(-2))from 2019 to 2022.The results showed that increasing N rate positively influenced yield while decreasing lodging resistance in all genotypes.Increasing plant density(D2-D3)enhanced lodging resistance and yield in sca^(HS5) and+/sca^(HS5),but reduced yield in ^(HS5).Compared to the two parents,+/sca^(HS5) exhibited moderate expressions of IAA3,GH3.15,and SAUR30 in stems under N2D3,resulting in reduced plant height and increased compactness.Additionally,+/sca^(HS5) had a thicker silique layer than ^(HS5) by 14.7%,and it had a significant correlation between branch height/angle and yield.Increasing N rate led to increased lignin and pectin contents,while cellulose content decreased.Increasing plant density resulted in greater stem cellulose content and CSLA3/7 expression in sca^(HS5) and+/sca^(HS5),but decreased in ^(HS5).Compared to ^(HS5),+/sca^(HS5) exhibited higher expressions of ARAD1 and GAUT4,along with a 51.1%increase in pectin content,leading to improved lodging resistance under N2D3.Consequently,+/sca^(HS5) showed a 46.4%higher yield and 38.9%lodging resistance than ^(HS5) under N2D3,while sca^(HS5) demonstrated strong lodging resistance but lower yield potential.Overall,this study underscores the potential of utilizing auxin dwarf genes to optimize the trade-off between yield and lodging resistance in rapeseed and the possibility of maximizing yield potential by optimizing the plant architecture of+/sca^(HS5) through nitrogen reduction and dense planting.
基金supported by the National Natural Science Foundation of China(32100448,32070558,32061143030,32170636)Natural Science Foundation of Jiangsu Province(BK20210799)+2 种基金Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),the Seed Industry Revitalization Project of Jiangsu Province(JBGS[2021]009)the Shanghai Science and Technology Agriculture Project([2022]No.1–6)the Project of Zhongshan Biological Breeding Laboratory(BM2022008-029)。
文摘In a study of DNA methylation changes in melatonin-deficient rice mutants,mutant plants showed premature leaf senescence during grain-filling and reduced grain yield.Melatonin deficiency led to transcriptional reprogramming,especially of genes involved in chlorophyll and carbon metabolism,redox regulation,and transcriptional regulation,during dark-induced leaf senescence.Hypomethylation of mCG and mCHG in the melatonin-deficient rice mutants was associated with the expression change of both protein-coding genes and transposable element-related genes.Changes in gene expression and DNA methylation in the melatonin-deficient mutants were compensated by exogenous application of melatonin.A decreased S-adenosyl-L-methionine level may have contributed to the DNA methylation variations in rice mutants of melatonin deficiency under dark conditions.
基金supported and funded by Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia(KFU241897).
文摘Rice(Oryza sativa L.)is one of the most important cereal crops in the world.Bakanae disease is a significant rice disease widely distributed in rice-growing regions worldwide.Therefore,the present investigation aimed to assess the optimal concentrations of paclobutrazol(PBZ)as a treatment for rice grains(cv.Sakha 108)to control bakanae disease,also evaluating its impact on grain germination,seedling growth parameters as well as disease index.Paclobutrazol concentrations had no significant impact on seed germination,regardless of whether the seeds were incubated with Fusarium fujikuroi or not.Application of PBZ,either alone or in combination with fungal pathogens,negatively impacted the rice seedlings’height.Paclobutrazol at 25,50 and 100 mg/L,combined with the fungal pathogen positively impacted root length.Paclobutrazol at 3 and 6 mg/L mitigated the adverse impact on chlorophyll pigments content in infected seedlings.The highest proline contents were achieved by 100 mg/L PBZ alone or in combination with fungal pathogens.It has been observed that the application of PBZ,either alone or in combination with a fungal pathogen,leads to the enhancement of catalase,peroxidase,and polyphenol oxidase activities.The median lethal concentration of PBZ was 0.874 mg/L;applying low concentrations of paclobutrazol effectively increased the percentage of fungal growth suppression.Application of PBZ,at higher concentrations(50 and 100 mg/L),decreased infection percentage and disease severity index(DSI)significantly.These findings suggest that PBZ can be an effective treatment for controlling bakanae disease and enhancing resistance in rice plants.
基金supported and funded by Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia,grant number(Grant A410).
文摘Twenty-four rice genotypes were examined to assess genetic variability,heritability,and correlations for seven-grain quality traits,eight nutritional elements,and protein.ANOVA revealed significant differences for the quality traits studied.For every trait under study,the phenotypic coefficient of variation was higher than the correspon-dence genotypic coefficient of variation.Heritability in a broad sense varied from 29.75%for grain length to 98.31%for the elongation trait.Hulling percentage recovery had a highly significant positive correlation with milling and head rice percentage.Consequently,milling percentage had a highly positive correlation with head rice percentage.In amylose percentage,all the genotypes belonged to low amylose except the Hassawi-1 variety,which had intermediate amylose content.Mineral nutrition contents of magnesium(Mg),sodium(Na),potas-sium(K),calcium(Ca),copper(Cu),manganese(Mn),zinc(Zn),iron(Fe),or protein percentage gave different variations for 24 rice genotypes under all the nutritional elements.Among the 24 genotypes,ten rice genotypes–HighNutrient-1,HighNutrient-2,HighNutrient-9,HighNutrient-8,HighNutrient-3,Hassawi-2,HighNutrient-7,HighNutrient-6,Hassawi-1,and HighNutrient-4–had the highest heist value for all nutritional and protein con-tents,and could be used as a donor to improving new varieties.There was a positive and significant correlation between magnesium Mg,K,Zn and Fe.Consequently,K had a positive correlation with zinc Zn,Fe,and protein percentage.Clustering analysis was divided into two groups:thefirst group included all genotypes rich in nutri-ents,while the remaining genotypes with low nutritional content were included in the second group.
文摘Barley production has been constrained by various factors,of which loose smut(Ustilago nuda)is the main biotic factor.Molecular and agronomical screening analyses were evaluated to study the similarity levels and marker assisted selection associated with resistance to loose smut among eight barley genotypes using sequence-related amplified polymorphism(SRAP).Agronomical parameters during two consecutive 2021 and 2022 seasons were studied to identify their reaction to loose smut.The results exhibited significant differences among all studied genotypes for all studied characters,and the highest mean values for all studied traits were detected in Giza 136 and Giza 137.Six SRAP selected primer combinations were amplified and gave 56 total fragments,where primer combination me1+em3 gave the highest polymorphism(100%)and the highest polymorphic information content of PIC was 0.96.The dendrogram of SRAP markers had clustered all studied genotypes into two main clusters.Cluster I includes all the resistance genotypes Giza 136,Giza 137,Giza 123,Giza 132,Giza 138 and Line 2.However,cluster II includes only Line 1 and Line 3 as susceptible genotypes.Thus,SRAP marker could be efficiently used to assess genetic variation among barley genotypes and useful for barley germplasm management in terms of biodiversity protection and design of new crosses for loose smut breeding programs,and seed dressings are commonly used to prevent infected seed from developing smutted heads.Some effective seed dressings include Triticonazole,Black seed oil and gamma rays 150,200,250 Gy.The tested fungicide was highly effective in controlling the disease and gave more than 99%disease control with high grain yield while nature product as black seed oil and utilizing radiation gamma ray 250 GY was the most effective.Utilizing radiation and natural ingredients to manage loose barley smut is economical.
基金Authors extend their appreciation to Deanship of Scientific Research,King Faisal University,Saudi Arabia,for supporting this research(NA000112).
文摘Monogenic lines,which carried 23 genes for blast resistance were tested and used donors to transfer resistance genes by crossing method.The results under blast nursery revealed that 9 genes from 23 genes were susceptible to highly susceptible under the three locations(Sakha,Gemmeza,and Zarzoura in Egypt);Pia,Pik,Pik-p,Piz-t,Pita,Pi b,Pi,Pi 19 and Pi 20.While,the genes Pii,Pik-s,Pik-h,Pi z,Piz-5,Pi sh,Pi 3,Pi 1,Pi 5,Pi 7,Pi 9,Pi 12,Pikm and Pita-2 were highly resistant at the same locations.Clustering analysis confirmed the results,which divided into two groups;the first one included all the susceptible genes,while the second one included the resistance genes.In the greenhouse test,the reaction pattern of five races produced 100%resistance under artificial inoculation with eight genes showing complete resistance to all isolates.The completely resistant genes:Pii,Pik-s,Piz,Piz-5(=bi2)(t),Pita(=Pi4)(t),Pita,Pi b and Pi1 as well as clustering analysis confirmed the results.In the F1 crosses,the results showed all the 25 crosses were resistant for leaf blast disease under field conditions.While,the results in F2 population showed seven crosses with segregation ratio of 15(R):1(S),two cross gave segregated ratio of 3 R:1 S and one gave 13:3.For the identification of blast resistance genes in the parental lines,the marker K3959,linked to Pik-s gene and the variety IRBLKS-F5 carry this gene,which was from the monogenic line.The results showed that four genotypes;Sakha 105,Sakha 103,Sakha 106 and IRBLKS-F5 were carrying Pik-s gene,while was absent in the Sakha 101,Sakha 104,IRBL5-M,IRBL9-W,IRBLTACP1 and IRBL9-W(R)genotypes.As for Pi 5 gene,the results showed that it was present in Sakha 103 and Sakha 104 varieties and absent in the rest of the genotypes.In addition,Pita-Pita-2 gene was found in the three Egyptian genotypes(Sakha 105,Sakha 101 and Sakha 104)plus IRBLTACP1 monogenetic.In F2 generation,six populations were used to study the inheritance of blast resistance and specific primers to confirm the ratio and identify the resistance genes.However,the ratios in molecular markers were the same of the ratio under field evaluation in the most population studies.These findings would facilitate in breeding programs for gene pyramiding and gene accumulation to produce durable resistance for blast using those genotypes.
文摘Rice(Oryza sativa L.)is an important cereal crop,consumed as a staple by more than 50%of the global population(Gathala et al,2011).In Asian countries,the rice production increased to 695.5 million tones in the last years,and this increasing was caused by the main source of income and employment in these countries.Breeding high-yielding hybrid rice is a potentially promising solution for addressing food shortages caused by marked increases in the global population.Recent progress in DNA marker technology and the construction of linkage maps has enabled the detection of quantitative trait loci(QTLs)that regulate different agronomic traits applicable to breeding(Parida et al,2006;Zhang et al,2017).
基金supported by the National Key Research and Development Program of China (2016YFD0300407)the earmarked fund for the China Agriculture Research System (CARS-03)the Talented Young Scientist Program (TYSP) in China
文摘Wheat was the first crop grown in Egypt, and it remains highly important. Egypt is the largest wheat importer in the world and consumes an extensive amount of bread. It is imperative for wheat scientists to decrease the large gap between production and consumption. Wheat yields in Egypt increased 5.8-fold(6.7 billion kg) between 1961 and 2017 due to variety improvement and the use of better planting methods such as the raised bed method, ideal sowing date, surge flow irrigation and farm irrigation systems, laser levelling, fertilizers, and intercropping with raised beds. In this paper, the development of wheat production techniques and variety evolution over more than five decades in Egypt have been analyzed. In particular, we have focused on the technologies, cultural practices and causes for per unit area yield increase. The main purpose was to study the issues that have arisen during wheat production and to make recommendations for smart agricultural practices. In 1981, the yield was 3 300 kg ha–1 and through the improvement of varieties, expansion of agricultural land and the adoption of modern agricultural techniques yield reached 6 500 kg ha–1 by 2017. The production growth rate was 4.1% annually, and the total grain yield increased 4.3-fold, from 1.9 billion kg in 1981 to about 8.1 billion kg in 2017. The use of new improved varieties, new cultivation techniques, and modern irrigation techniques contributed to 97.0% of the increase in yield per unit area and 1.5% of the increase in yield was due to planting area expansion. Therefore, the increase in total yield mainly depended on the increase in yield per unit area. Wheat production in Egypt has been improved through the development of breeding and cultivation techniques. The use of these new techniques, the popularization of new high-quality seed varieties, and the use of the raised bed method instead of the old method of planting in basins have made the largest contributions to increased yield. In the future, wheat yield could be further increased by using the tridimensional uniform sowing mode and the development of wheat varieties that are resistant to rusts, deficit irrigation, and abiotic stress, that are highly adaptable to mechanized operation and have high yields. Based on our analysis, we propose the main technical requirements and measures to increase wheat yield in Egypt in the near future.
文摘Adjustment of planting geometry along with suitable maize cultivar can be a viable tool for maximizing land usage and net return. A two-year study was carried out at Serw Agricultural Experiments and Research Station, A.R.C., Domiate governorate, Egypt, during 2015 and 2016 seasons to evaluate intercropping cowpea with suitable maize cultivar and its planting geometry for maximizing land usage and net return. The treatments were the combinations between three maize cultivars (SC 30K08, TWC 310 and TWC 352) and three maize plant distributions (one plant/hill distanced at 25, 50 and 75 cm between hills, respectively). Ridge width with maize plant distribution formed maize planting geometry (25 cm × 140 cm, 50 cm × 140 cm and 75 cm × 140 cm). These treatments were compared in a split plot distribution in randomized complete block design with three replications. Maize cultivar SC 30K08 had the highest grain yield and its attributes compared with the other cultivars in both seasons. Also, maize planting geometry 25 cm × 140 cm resulted in the highest grain yield and its attributes compared with the other planting geometries in both seasons. Ear length, ear weight, 100-grain weight and grain yield/fad were affected significantly by maize cultivars × maize planting geometry. Intercropping cowpea with maize cultivar TWC 352 had the highest seed yield and its attributes compared with those intercropped with the other cultivars in both seasons. Also, maize planting geometry 75 cm × 140 cm resulted in the highest seed yield and its attributes through growing two cowpea rows between maize hills compared with the other maize planting geometries in both seasons. Number of pods/plant and seed yield/fad were affected significantly by maize cultivars × maize planting geometry. LER and LEC values of the intercrops were much greater than 1.00 and 0.25, respectively, for all the combinations indicating less land requirements of intercropping system than solid culture of both crops. Growing one row of maize cultivar TWC352 in both sides of bed 140 cm width with cowpea two rows in middle of the bed increased land productivity and net return compared with solid culture of maize.
基金funded by Bangladesh Agricultural University Research System(BAURES,Bangladesh Agricultural University,Mymensingh through the research project number:2018/597/BAU.Taif University Researchers Supporting Project number(TURSP-2020/120)Taif University,Taif,Saudi Arabia provided the APC of this publication.
文摘Seed priming has proved to be an effective pre-germination seed invigoration technique for different crops to improve seed and seedling performance under different abiotic stresses.In Bangladesh,winter rice is very often exposed to cold waves just after sowing in the nursery bed resulting in poor seed germination and seedling emergence,yellowish and thin seedlings production,and a very low survival rate.Seed priming may mitigate the cold stress during seed germination and seedling emergence and helps in the quality seedling production of winter rice.To evaluate the efficacy of different seed priming techniques in increasing seedling emergence,growth,vigor and survivability of winter rice cultivars under cold stress,a pot experiment was conducted at the Department of Agronomy,Bangladesh Agricultural University during December 2018 to January 2019.The experiment comprised two factors,(A)Winter rice variety namely,(i)BRRI dhan29 and (ii)BRRI dhan36;(B)Seed priming agent namely (i)Control(no priming),(ii)20000 ppm NaCl,(iii)30000 ppm NaCl,(iv)20000 ppm KCl,(v)30000 ppm KCl,(vi)20000 ppm CaCl2,(vii)30000 ppm CaCl2,(viii)50 ppm CuSO4,(ix)75 ppm CuSO_(4),(x)10000 ppm ZnSO4,(xi)15000 ppm ZnSO4,(xii)2 ppm Na2MoO4,(xiii)3 ppm Na2MoO4,(xiv)100 ppm PEG(Polyethylene glycol 4000)and (xv)150 ppm PEG.Seeds were sown on two different dates viz.,1st December and 1st January so that seedlings are exposed to cold stress at different stages.The experiment was laid out in a completely randomized design(CRD)with three replications.Results indicated that(in most of the cases)seed priming has a positive impact on seedling emergence rate(%),root length,shoot length,root shoot ratio,root dry weight,shoot dry weight,seedling dry weight and survival rate(%).Among the priming agents,KCl and CaCl2 performed best;while priming with NaCl and PEG showed no advantages over no priming for both the sowing dates.In general,BRRI dhan36 performed better than BRRI dhan29 in terms of seedling growth because of its higher tolerance to cold stress.But,both the varieties performed similarly in terms of emergence rate and survival rate.Thus,priming is an effective tool to increase seed germination,better seedling growth,and higher seedling survivability of winter rice under cold stress,and KCl(20000 ppm)or CaCl2(20000 ppm)can be considered as a viable priming agent.
基金The International Winter Wheat Improvement Program is supported by CRP WHEAT and Ministry of Food,Agriculture and Livestock of Turkey
文摘The study estimated genetic gain for yield and other traits in winter wheat released for irrigated environments in Turkey from 1963 to 2004. Yield trials including 14 varieties were grown in 16 environments from 2008 to 2012 in provinces of Konya, Eski?ehir, Ankara, and Edirne. The highest yields were achieved by recent varieties Kinaci-97(5.48 t ha^(-1)),Cetinel-2000(5.39 t ha^(-1)), Alpu-2001(5.44 t ha^(-1)), Ahmetaga(5.35 t ha^(-1)), and Ekiz-2004(5.42 t ha^(-1)) compared to older varieties Yektay-406(4.17 t ha^(-1)) and Bezostaya-1(4.27 t ha^(-1))released in the 1960 s. The progress reached in grain yield in 20 years was 1.16 t ha^(-1)or58 kg ha^(-1)(1.37%) per year. This gain was mainly achieved through shorter plant height and increased harvest index. There was no clear tendency of changes in specific yield components demonstrating that new high-yielding varieties may have different ways to reach their yield potentials. The yield gains were accompanied by improved stripe rust and leaf rust resistances primarily based on adult plant resistance genes. The grain quality of the new varieties did not deteriorate over time although most of them were inferior to the bread-making quality check Bezostaya-1, a feature that may require attention in future breeding.
基金carried out in the framework of the‘Perm Veg’project(2007-2013)part of the strategic research program KB1"Global Food Security:Scarcity and Transition"which was funded by the Dutch Ministry of Economic Affairs,and carried out by Wageningen University and Research Centre
文摘Vegetable production in South East Asia often is in rotation with flooded rice. The puddling of the soil with flooded rice production may result in unfavourable soil conditions for the subsequent production of dry land crops. To establish whether permanent vegetable production results in favourable soil conditions for vegetables, the effects of five different permanent vegetable production systems and a system of vegetable production in rotation with flooded rice on soil properties after flooded rice were studied in a 2-year field experiment. Bulk density at 0.05–0.10 m depth layer decreased with permanent vegetable production and vegetable production in rotation with flooded rice. The decrease in bulk density was influenced by the application of organic manure and rice husks, and especially by the number of crops cultivated, suggesting that frequency of soil tillage had a major effect on bulk density. Ploughing with buffalo traction after flooded rice, in combination with construction of raised beds, could reduce or totally eliminate negative effects of puddling on soil structure. Bulk density at 0.15–0.20 m soil depth was not influenced. Soil acidity decreased significantly in all systems. Soil organic carbon increased in all systems, but significant increase was only found in two permanent vegetable production systems. Available phosphorus(P) significantly increased in two permanent vegetable production systems, with a positively correlation to the amount of P applied. The significant decrease in bulk density and increase in p H(H2O), after only 2 years, showed that soil conditions after flooded rice could be improved in a short time under intensive vegetable production.
文摘Ten Egyptian barley genotypes (2 commercial varieties and 8 breeding lines) were cultivated under normal condition at the Experimental Farm of Sakha Agricultural Research station and exposed to salinity stress condition at the Experimental Farm of El-hosainia plain Agricultural Research station, Elsharkia Governorate, Egypt, in an attempt to identify the relative salinity tolerant genotypes. A susceptibility index (SI) was used to estimate the relative stress loss because it accounted for variation in yield potential and stress intensity. Giza 123, Line-1, Line-5, Line-6 and Line-8 genotypes were considered as saline tolerant genotypes on the basis of their highly tolerance indices values. Barley genotypes were characterized by seven SSR markers and three SCoT primers in different combinations to discern the extent of genetic variation and develop a fingerprinting key. Normal SCoT reactions amplify single segments of DNA which are 15- to 19-mer long. A new strategy was used to increase SCoT potential in genetic diversity studies by using two and three different primer combinations per reaction. Amplification products scored a polymorphism percentage of 94.44% for Triple-SCoT and 90.91% for SSR, while the average no. of polymorphic fragments/primer was 17 and 7.14 in the two marker systems, respectively. On the other side, Triple-SCoT exhibited the highest average number of positive and negative genotype-specific markers. The cluster analysis of the studied genotypes using these different marker systems revealed four dendrograms varied in their topology. The dendrogram based on Triple-SCoT data exhibited the closest relationships to those illustrated by SSR dendrogram.
文摘Water deficit is a limiting factor in sesame cultivars yield,thus identification of genetic mechanisms of sesame traits under water stress conditions is essential to development of water stress tolerant genotypes.Hence,the triple test cross(TTC)analysis in F_(2) population of the sesame cross(NA_(76)NA_(54))was used.Since,30 TTC families with their 13 respective parents were evaluated during the summer season 2020 under two irrigation treatments.i.e.,normal irrigation with 5952.38 m^(3) hm^(-2) of applied water,and water stress with 2976.19 m^(3) hm^(-2) of applied water.This was implemented at the experimental field of Kafr El Hamam/Sharkia,Agricultural Research Station,Agricultural Research Center(ARC),Egypt.Additive(D)and dominance(H)gene actions as well as epistatic and its two components of additiveadditive,additivedominance plus dominancedominance were involved in the inheritance of all studied traits under both irrigation treatments and their combined analysis.The degree of dominance(H/D)^(0.5) verified the presence of partial dominance in most cases.The correlation coefficient between sums(additive)and differences(dominance)was non-significant,showing that dominant genes between lines were ambidirectional.The moderately water stress tolerant TTC families as the best selected families were found in families 22,25,10,12,and 15.Therefore,postponing selection in these selected families to later segregating generations for all studied traits would be effective to exploit the positive effects of additiveadditive epistasis.
基金supported by the Ministry of Foreign Affairs, Directorate General for Development Cooperation, Italy and National Research Council, Rome, Italy (Grant No. 1654)
文摘The response of rice to salt stress(200 mmol/L NaCl)was investigated at the transcription level in Egyptian varieties Giza 177(salt sensitive variety)and Giza 178(salt tolerant variety).We applied a genome-wide RNA-Seq transcriptome study at 21-day-old seedlings of both varieties,exposed or not to salt stress for 24 h.
文摘Differences in floral morphologies affect pollination behaviour in many flowering plants. In the genus Oryza, several differences in the size of floral organs are known. In this study, we focused on the differences in the size of floral organs between common cultivated rice, Oryza sativa L. and its wild ancestor, O. rufipogon. We compared floral morphologies between cultivated rice O. sativa cv. Nipponbare and O. rufipogon W630. We first evaluated temporal changes in filament and anther lengths. W630 had longer filaments with rapid elongation within 15 min after spikelet opening. W630 also had longer anthers than Nipponbare, and size of anther was consistent throughout all time examined. We also analysed other six floral traits, and found that W630 had higher stigma and style length, as well as lemma and palea length, but lower lemma and palea width. Quantitative trait locus (QTL) analysis was performed to identify the loci controlling these floral traits, using backcross recombinant inbred lines derived from a cross between Nipponbare and W630. A total of 11 significant QTLs were identified. Of these, two pairs of QTLs for lemma and palea length and one pair for lemma and palea width overlapped, suggesting that common genetic factors may be the reason for the differences in these traits. In addition, we performed QTL analysis for grain size, and found that QTLs for grain size coincided with those for lemma and palea size, indicating that grain size is partly controlled by glume capacity. The QTLs identified in this study will be informative for understanding genetic changes associated with rice domestication.
文摘The author regrets that the affiliated institution of Ibthal S.ElDemardash should be“Department of Genetics and Cytology,National Research Centre,Cairo,Egypt”instead of“Department of Genetics and Cytology,National Research Center,Cairo,Egypt”.The author would like to apologize for any inconvenience caused.
文摘A two-year study was carried out at the Agricultural Experiments and Research Station, Faculty of Agriculture, Cairo University, Giza, Egypt, during 2015/2016 and 2016/2017 seasons to evaluate seed yield and quality of two faba bean cultivars to different rates of amino acids compounds under narrow and wide ridges. Two faba bean cultivars Sakha 1 and Masr 3 were grown in two ridge widths 60 and 120 cm and received five rates of amino acids compounds (Power mix at rates 1.5 cm⋅L−1 “Pm1” and 3.0 cm⋅L−1 “Pm2”, Super mix at rates of 3.5 cm⋅L−1 “Sm1” and 7.0 cm⋅L−1 “Sm2”, as well as, water only “control treatment”). The treatments laid out in a split split plot distribution in randomized complete blocks design in three replications were used. Ridge width was randomly assigned to the main plots, faba bean cultivars were allocated in sub-plots and amino acids compounds were allocated in sub sub-plots. Shoot chemical composition and photosynthesis pigments, as well as, chemical composition in seed traits of faba bean were tested in the laboratories of Plant Physiology Section, Agricultural Botany Department, Faculty of Agriculture, Cairo University, Giza, Egypt. Wide ridge had higher nitrogen (N), phosphorus (P), potassium (K), total sugars and total free amino acids concentrations in faba bean shoot and seeds in addition to chlorophyll a (Chl a) and chlorophyll b (Chl b) in faba bean shoot, as well as, most seed yield attributes than those of the narrow one. Faba bean cultivar Sakha 1 had higher values of seed yield, yield attributes and quality than Masr 3 that achieved higher seed antioxidants. Also, amino acids compounds affected significantly all studied traits of faba bean plants. Pm2 recorded the highest values of chemical composition in faba bean shoot and seeds. Moreover, Pm2 gave the highest seed yield and yield attributes compared to others. The interaction between ridge width and faba bean cultivar was significant for total sugars and carotenoids concentrations in faba bean shoot and 100-seed weight, as well as, seed N content. Also, the interaction between ridge width and amino acids compound had significant effects on shoot and seed N, P, K concentrations, number of pods plant−1 and seed yield ha−1, as well as, total soluble phenols and total free amino acids concentrations. Moreover, the interaction between faba bean cultivar and amino acids compound had significant effects on shoot Chl a and b concentrations, number of seeds pod−1 and seed yield plant−1, as well as, seed N, total soluble phenols and total free amino acids concentrations. Wide ridge x faba bean cultivar x amino acids compound interaction had significant effects on photosynthesis pigments, number of seeds pod−1 and seed yield ha−1, as well as, seed N, total sugars, total soluble phenols and total free amino acids concentrations. High seed yield and quality were recorded by growing faba bean cultivar Sakha 1 in wide ridge with foliar spraying at rate of 3.0 cm⋅L−1 of power mix. On the other hand, growing faba bean cultivar Masr 3 in narrow ridge with foliar spraying at rate of 3.5 cm⋅L−1 of super mix had good antioxidants in faba bean seeds.
