This study explored the preventive effects of Orobanche crenata extract(OCE)against myocardial infarction.LCMS/MS profiling revealed the presence of 27 metabolites,including phenolic acids,flavonoids and phenylethanoi...This study explored the preventive effects of Orobanche crenata extract(OCE)against myocardial infarction.LCMS/MS profiling revealed the presence of 27 metabolites,including phenolic acids,flavonoids and phenylethanoid glycosides.In vitro,OCE exhibited significant antioxidant activity via DPPH and ABTS assays,attributed to its noteworthy TPC=48.02 mg GAE/g and TFC=20.83 mg QE/g.In vivo,rats were subjected to isoproterenol injections(29th and 30th days),following pretreatments with OCE(20 and 60 mg/kg)or Pidogrel(Pid 2 mg/kg).Most of the changes were dose-dependent,with a pronounced impact at 60 mg/kg compared to 20 mg/kg.Compared to ISO-treated group,the pretreatment with OCE at(60 mg/kg bw)reduced CK-MB,LDH,AST and cTn-I by 45%,43.5%,48.3%and 67%,respectively(p<0.0001)and normalized the lipid profile,by alleviating LDL-C levels by 59%and enhancing HDL-C levels by 67%(p<0.01).OCE promoted antioxidant enzymes(CAT,SOD and GPX)by 53%,168%and 209%,respectively,reducing lipid peroxidation by 65%(p<0.0001).Additionally,the findings revealed the inhibitory activity of the extract against ACE and the reduction of plasmatic fibrinogen level,indicating an anti-remodeling activity.Moreover,Molecular docking revealed strong binding affinities(-7 and-9.8 kcal/mol)of orobanchoside,isorhamnetin rhamnosyl glucoside,crenatoside,quercetin coumaroyl glucoside and apigenin with cardiotoxicity related proteins:ACE,TGF-β1,PDI,MAPK14 and CaMK II.Overall,OCE represents a potential source of cardioprotective agents,supported by its richness in phenolic compounds,capable of reducing cardiac damage by countering oxidative stress,inflammation,fibrosis,calcium overload,cardiac remodeling.展开更多
Melilotus indicus is a plant known for its remarkable pharmacological properties and has been traditionally used to treat various pathological conditions.This study explored the phytochemical composition and evaluated...Melilotus indicus is a plant known for its remarkable pharmacological properties and has been traditionally used to treat various pathological conditions.This study explored the phytochemical composition and evaluated the therapeutic potential of M.indica seed extract(MIS)in mitigating lithiasis in Wistar rats.Using LC-MS/MS^(2),the detected metabolites were grouped into hydroxybenzoic acids(2),hydroxycinnamic acids(11),flavones(13),flavonols(13),saponins(10),organic acids(7),fatty acids(9),jasmonates(3),and a sugar.The experimental design included the administration of two MIS doses to rats,followed by the administration of ethylene glycol(0.75%w/v)to induce urolithiasis.The results demonstrated that MIS1(20 mg/kg)and MIS^(2)(40 mg/kg)significantly reduced urinary calcium oxalate crystals by 38.5%and 59.2%,respectively,following ethylene glycol-administration.The results suggested the potential of MIS as a natural diuretic agent for helping to remove crystal debris.The administration of MIS1 and MIS^(2)mitigated the renal oxidative stress induced by ethylene glycol,as evidenced by the reduction in malondialdehyde(MDA)levels(by 29%and 45%,respectively)and the enhancement of antioxidant enzyme markers,with increased activities of SOD(by 20.7%and 37.5%,respectively)and CAT(by 19%for MIS^(2)only).The above results were confirmed by the dosage of plasma parameters markers and histopathological evaluations.展开更多
Due to the side effects of chemical drugs,recent studies have targeted new therapeutic approach using natural bioactive molecules.The purpose of the current study was to explore,for the first time,the cardiopreventive...Due to the side effects of chemical drugs,recent studies have targeted new therapeutic approach using natural bioactive molecules.The purpose of the current study was to explore,for the first time,the cardiopreventive capacity of Eucalyptus torquata seeds extract(ETS)against myocardial infarction induced by Isoproterenol(ISO)in rats.The phytochemical profile was also targeted.The LC-MS/MS analysis revealed the presence of 53 metabolites belonging to hydroxybenzoic acids,lignans,flavonoids,phloroglucinols,oxocins,organic acids,fatty acids,amino acids,and sugars.The in vivo findings showed that ETS supplementation for 28 days significantly attenuated the cardiotoxicity in adult rats by ameliorating the electrocardiographic pattern(ECG),and reducing the activity of LDH,AST,CK-MB,and troponin-T content compared to the infracted group.Furthermore,ETS reduced the cardiac oxidative stress generated by ISO injection via inhibiting lipid peroxidation and ameliorating the activities of enzymatic antioxidants(SOD,and CAT).Additionally,ETS supplementation attenuated the DNA fragmentation and improved the ultrastructural changes in cardiac tissue.The amelioration of the lipid level(TG,TC,LDL-C,and HDL-C),highlighted the antihyperlipidemic action of ETS.The positive effect of the studied extract against ISO-induced cardiac damage could be related to the bioactive compounds,known as effective antioxidant molecules.The current study provides a preliminary pharmacological support for a potential medicinal application of ETS against cardiovascular pathology.展开更多
文摘This study explored the preventive effects of Orobanche crenata extract(OCE)against myocardial infarction.LCMS/MS profiling revealed the presence of 27 metabolites,including phenolic acids,flavonoids and phenylethanoid glycosides.In vitro,OCE exhibited significant antioxidant activity via DPPH and ABTS assays,attributed to its noteworthy TPC=48.02 mg GAE/g and TFC=20.83 mg QE/g.In vivo,rats were subjected to isoproterenol injections(29th and 30th days),following pretreatments with OCE(20 and 60 mg/kg)or Pidogrel(Pid 2 mg/kg).Most of the changes were dose-dependent,with a pronounced impact at 60 mg/kg compared to 20 mg/kg.Compared to ISO-treated group,the pretreatment with OCE at(60 mg/kg bw)reduced CK-MB,LDH,AST and cTn-I by 45%,43.5%,48.3%and 67%,respectively(p<0.0001)and normalized the lipid profile,by alleviating LDL-C levels by 59%and enhancing HDL-C levels by 67%(p<0.01).OCE promoted antioxidant enzymes(CAT,SOD and GPX)by 53%,168%and 209%,respectively,reducing lipid peroxidation by 65%(p<0.0001).Additionally,the findings revealed the inhibitory activity of the extract against ACE and the reduction of plasmatic fibrinogen level,indicating an anti-remodeling activity.Moreover,Molecular docking revealed strong binding affinities(-7 and-9.8 kcal/mol)of orobanchoside,isorhamnetin rhamnosyl glucoside,crenatoside,quercetin coumaroyl glucoside and apigenin with cardiotoxicity related proteins:ACE,TGF-β1,PDI,MAPK14 and CaMK II.Overall,OCE represents a potential source of cardioprotective agents,supported by its richness in phenolic compounds,capable of reducing cardiac damage by countering oxidative stress,inflammation,fibrosis,calcium overload,cardiac remodeling.
基金Supporting Project number RSP2025R17,King Saud University,Riyadh,Saudi ArabiaRamon y Cajal Grant,RYC2020-030546-I/AEI/10.13039/501100011033。
文摘Melilotus indicus is a plant known for its remarkable pharmacological properties and has been traditionally used to treat various pathological conditions.This study explored the phytochemical composition and evaluated the therapeutic potential of M.indica seed extract(MIS)in mitigating lithiasis in Wistar rats.Using LC-MS/MS^(2),the detected metabolites were grouped into hydroxybenzoic acids(2),hydroxycinnamic acids(11),flavones(13),flavonols(13),saponins(10),organic acids(7),fatty acids(9),jasmonates(3),and a sugar.The experimental design included the administration of two MIS doses to rats,followed by the administration of ethylene glycol(0.75%w/v)to induce urolithiasis.The results demonstrated that MIS1(20 mg/kg)and MIS^(2)(40 mg/kg)significantly reduced urinary calcium oxalate crystals by 38.5%and 59.2%,respectively,following ethylene glycol-administration.The results suggested the potential of MIS as a natural diuretic agent for helping to remove crystal debris.The administration of MIS1 and MIS^(2)mitigated the renal oxidative stress induced by ethylene glycol,as evidenced by the reduction in malondialdehyde(MDA)levels(by 29%and 45%,respectively)and the enhancement of antioxidant enzyme markers,with increased activities of SOD(by 20.7%and 37.5%,respectively)and CAT(by 19%for MIS^(2)only).The above results were confirmed by the dosage of plasma parameters markers and histopathological evaluations.
基金the Deanship of Scientific Research at King Khalid University for funding this work through Small Groups Project under grant number S.R.G.P./288/44M.d.M.C.thanks the support by the Ministry of Science and Innovation of Spain through the Ramón y Cajal grant RYC2020-030546-I/AEI/10.13039/501100011033the European Social Fund.
文摘Due to the side effects of chemical drugs,recent studies have targeted new therapeutic approach using natural bioactive molecules.The purpose of the current study was to explore,for the first time,the cardiopreventive capacity of Eucalyptus torquata seeds extract(ETS)against myocardial infarction induced by Isoproterenol(ISO)in rats.The phytochemical profile was also targeted.The LC-MS/MS analysis revealed the presence of 53 metabolites belonging to hydroxybenzoic acids,lignans,flavonoids,phloroglucinols,oxocins,organic acids,fatty acids,amino acids,and sugars.The in vivo findings showed that ETS supplementation for 28 days significantly attenuated the cardiotoxicity in adult rats by ameliorating the electrocardiographic pattern(ECG),and reducing the activity of LDH,AST,CK-MB,and troponin-T content compared to the infracted group.Furthermore,ETS reduced the cardiac oxidative stress generated by ISO injection via inhibiting lipid peroxidation and ameliorating the activities of enzymatic antioxidants(SOD,and CAT).Additionally,ETS supplementation attenuated the DNA fragmentation and improved the ultrastructural changes in cardiac tissue.The amelioration of the lipid level(TG,TC,LDL-C,and HDL-C),highlighted the antihyperlipidemic action of ETS.The positive effect of the studied extract against ISO-induced cardiac damage could be related to the bioactive compounds,known as effective antioxidant molecules.The current study provides a preliminary pharmacological support for a potential medicinal application of ETS against cardiovascular pathology.
