BACKGROUND Gamification,the use of game materials in education,has been used in several scientific fields,and studies have demonstrated its effectiveness in student comprehension.AIM To evaluate whether the success of...BACKGROUND Gamification,the use of game materials in education,has been used in several scientific fields,and studies have demonstrated its effectiveness in student comprehension.AIM To evaluate whether the success of 4th-and 5th-grade dentistry students increases when using the gamification applications Kahoot!and Mentimeter.METHODS An experimental design was used to measure the effect of gamification applications on students who were taught using the traditional method.For this purpose,Mentimeter and Kahoot!applications were used in this experimental study.Three groups of 4th-and 5th-grade students were formed:Mentimeter,Kahoot!,and control groups.The effect of gamification applications on achievement was evaluated by administering a post-test to these groups after the instructional process.One-way analysis of variance and t-tests were used for statistical analyses.RESULTS A total of 35.5%of the students were in the Mentimeter group,29.8%in the Kahoot!group,and 34.6%in the control group.Of the total number of students,54.8%were female and 45.2%were male,while 52.2%were 5th-grade students and 47.8%were 4th-grade students.The overall grade point average was found to be 66.65(range,24-100).There was a statistically significant difference in average success scores between students of different groups(P=0.003<0.05)and grades(P=0.036<0.05).The average success score was the highest in the Mentimeter group(71.64)and the lowest in the Kahoot!group(62.15).In the control group,this score was 65.41.The average success score was higher for students in the 5thgrade(68.16)and for female students(68.84).CONCLUSION Gamification has a great potential in the education of dentomaxillofacial radiology students.Mentimeter outperformed both the Kahoot!and the traditional method in terms of average student success rates.展开更多
BACKGROUND Robotic assistance is increasingly used for donor and recipient hepatectomy in liver transplantation,yet existing evidence is fragmented and variably indirect.AIM To evaluate clinical outcomes,surgical perf...BACKGROUND Robotic assistance is increasingly used for donor and recipient hepatectomy in liver transplantation,yet existing evidence is fragmented and variably indirect.AIM To evaluate clinical outcomes,surgical performance,and economic effects of robotic-assisted donor and recipient hepatectomy in the transplant pathway.METHODS Following Preferred Reporting Items for Systematic reviews and Meta-Analyses 2020 and a priori registration,systematic reviews were included with or without meta-analysis.Four databases were searched through July 2025.Methodological quality was appraised with a measurement tool to assess systematic reviews(AMSTAR 2),and certainty was graded with grading of recommendations assessment,development and evaluation(GRADE).Evidence overlap was calculated via a citation-matrix-based corrected covered area(CCA).Effect sizes were prespecified as risk ratios(RR)for dichotomous outcomes and mean differences for continuous outcomes.RESULTS Five reviews met the inclusion criteria,four with meta-analyses and one consensus review used only for context.Donor(direct)findings were more favorable for robotics in terms of estimated blood loss(≈-117 mL)and length of stay(≈-0.6 days),although with longer operative time(≈+105 minutes).Absolute risks for donor complications were not estimable from ratio-only data.Recipient(indirect)meta-analysis indicated robotics to be favorable in terms of conversion(RR≈0.41)and severe morbidity(RR≈0.81),with a trend toward lower overall morbidity(RR≈0.92)and no difference in 30-day mortality.Differences in length of stay and operative time were small and heterogeneous.Economic evidence(indirect,network meta-analysis)suggested higher procedural costs for robotic vs laparoscopic intervention,but lower hospitalization costs vs open intervention,with laparoscopy the least expensive overall.AMSTAR 2 ratings were moderate-to-high across the reviews,GRADE certainty was low for key donor continuous outcomes,and low-to-moderate for recipient and economic outcomes.Overlap was slight(graded-corpus CCA=0.0%;including a contextual non-transplant review increased CCA to≈1.25%).CONCLUSION Robotic donor hepatectomy confers perioperative advantages at the cost of longer operative time.Recipient and economic findings are indirect and considered hypothesis-generating.Transplant-specific,prospective comparisons using a minimum standardized dataset and uniform outcome definitions are needed to resolve remaining uncertainties and to clarify the cost-utility correlation.展开更多
Cone-beam computed tomography(CBCT) was developed and introduced specifically for dento-maxillofacial imaging. CBCT possesses a number of advantages over medical CT in clinical practice, such as lower effective radiat...Cone-beam computed tomography(CBCT) was developed and introduced specifically for dento-maxillofacial imaging. CBCT possesses a number of advantages over medical CT in clinical practice, such as lower effective radiation doses, lower costs, fewer space requirements,easier image acquisition, and interactive display modes such as mutiplanar reconstruction that are applicable to maxillofacial imaging. However, the disadvantages of CBCT include higher doses than two-dimensional imaging; the inability to accurately represent the internal structure of soft tissues and soft-tissue lesions; a limited correlation with Hounsfield Units for standardized quantification of bone density; and the presence of various types of image artifacts, mainly those produced by metal restorations. CBCT is now commonly used for a variety of purposes in oral implantology, dentomaxillofacial surgery, image-guided surgical procedures, endodontics, periodontics and orthodontics. CBCT applications provide obvious benefits in the assessment of dentomaxillofacial region, however; it should be used only in correct indications considering the necessity and the potential hazards of the examination.展开更多
BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors...BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors is their penetration of neighboring tissues,such as lymphatic and blood arteries,due to the tumor cells'capacity to break down the extracellular matrix(ECM).Matrix metalloproteinases(MMPs)constitute a family of proteolytic enzymes that facilitate tissue remodeling and the degradation of the ECM.MMP-9 and MMP-13 belong to the group of extracellular matrix degrading enzymes and their expression has been studied in OSCC because of their specific functions.MMP-13,a collagenase family member,is thought to play an essential role in the MMP activation cascade by breaking down the fibrillar collagens,whereas MMP-9 is thought to accelerate the growth of tumors.Elevated MMP-13 expression has been associated with tumor behavior and patient prognosis in a number of malignant cases.AIM To assess the immunohistochemical expression of MMP-9 and MMP-13 in OSCC.METHODS A total of 40 cases with histologically confirmed OSCC by incisional biopsy were included in this cross-sectional retrospective study.The protocols for both MMP-9 and MMP-13 immunohistochemical staining were performed according to the manufacturer’s recommendations along with the normal gingival epithelium as a positive control.All the observations were recorded and Pearson’sχ²test with Fisher exact test was used for statistical analysis.RESULTS Our study showed no significant correlation between MMP-9 and MMP-13 staining intensity and tumor size.The majority of the patients were in advanced TNM stages(III and IV),and showed intense expression of MMP-9 and MMP-13.CONCLUSION The present study suggests that both MMP-9 and MMP-13 play an important and independent role in OSCC progression and invasiveness.Intense expression of MMP-9 and MMP-13,irrespective of histological grade of OSCC,correlates well with TNM stage.Consequently,it is evident that MMP-9 and MMP-13 are important for the invasiveness and progression of tumors.The findings may facilitate the development of new approaches for evaluating lymph node metastases and interventional therapy techniques,hence enhancing the prognosis of patients diagnosed with OSCC.展开更多
The Ottoman-Turkish medicine and dentistry was born and developed in the lands of Anatolia. The most mature period of the Islamic medicine was in the Ottoman Empire on the lands of Anatolia. While inheriting the Turki...The Ottoman-Turkish medicine and dentistry was born and developed in the lands of Anatolia. The most mature period of the Islamic medicine was in the Ottoman Empire on the lands of Anatolia. While inheriting the Turkish-Islamic science and culture structure and the ancient age and Old Greek science, it was integrated with the medicine philosophy here. In the 13th and 15th centuries, in Ottomans, the development of the dentistry was on the issues of the dental diseases and periodontology. In the 14th and 15th centuries, it was observed in the medicine books of the Turkish physician writers that they were also dealing with the child dentistry as issues. That the dental medicine took place in the works of art written between the 15th and 19th century shows the existence of the surgeons realizing the tooth extraction. With the opening of the first dentist school in the year of 1909, the training of dentistry started. In this study, the issue of the development and training of the dentistry in the Ottomans among the 13th-19th centuries were dealt with the outlines. Also, the first examples and fundamental works of art of the dentistry literature from the Ottoman Empire to the Republican period will be told shortly.展开更多
Epilepsy has direct negative effects on sufferers’ general dental condition and oral health, both of which are further affected by inadequate oral hygiene;poor oral hygiene itself is often also caused by epilepsy-rel...Epilepsy has direct negative effects on sufferers’ general dental condition and oral health, both of which are further affected by inadequate oral hygiene;poor oral hygiene itself is often also caused by epilepsy-related poor health. Consequently, tooth loss, caries and periodontal disease occur increasingly often in epilepsy sufferers and they need more dental treatment. However, in fact the epileptic patients can receive fewer and simpler treatment modalities. The aim of this study was to review and synthesize recent studies on dental treatment in epilepsy patients and to mention potential triggers for seizures in dental practice.展开更多
BACKGROUND In the absence of effective antimicrobials,transplant surgery is not viable,and antirejection immunosuppressants cannot be administered,as resistant infections compromise the life-saving goal of organ trans...BACKGROUND In the absence of effective antimicrobials,transplant surgery is not viable,and antirejection immunosuppressants cannot be administered,as resistant infections compromise the life-saving goal of organ transplantation.AIM To evaluate the efficacy of antimicrobials in preventing resistance in solid organ transplant recipients.METHODS A systematic review was conducted using a search methodology consistent with the preferred reporting items for systematic reviews and meta-analyses.This review included randomized clinical trials that evaluated the efficacy of antimicrobial agents(prophylactic or therapeutic)aimed at preventing antimicrobial resistance.The search strategy involved analyzing multiple databases,including PubMed/MEDLINE,Web of Science,Embase,Scopus,and SciELO,as well as examining gray literature sources on Google Scholar.A comprehensive electronic database search was conducted from the databases’inception until May 2024,with no language restrictions.RESULTS After the final phase of the eligibility assessment,this systematic review ultimate-ly included 7 articles.A total of 2318 patients were studied.The most studied microorganisms were cytomegalovirus,although vancomycinresistant enterococci,Clostridioides difficile,and multidrug-resistant Enterobacterales were also analyzed.The antimicrobials used in the interventions were mainly maribavir,valganciclovir,gancic-lovir,and colistin-neomycin.Of concern,all clinical trials showed significant proportions of resistant microorga-nisms after the interventions,with no statistically significant differences between the groups(mean resistance 13.47%vs 14.39%),except for two studies that demonstrated greater efficacy of maribavir and valganciclovir(mean resistance 22.2%vs 41.1%in the control group;P<0.05).The total reported deaths in three clinical trials were 75,and there were 24 graft rejections in two studies.CONCLUSION All clinical trials reported significant proportions of antimicrobial-resistant microorganisms following interventions.More high-quality randomized clinical trials are needed to corroborate these results.展开更多
Oral and maxillofacial diagnostic imaging is of paramount importance in dental clinical diagnosis,treatment planning,and follow-up procedures.Periapical ra-diographic examination and numerous panoramic systems are use...Oral and maxillofacial diagnostic imaging is of paramount importance in dental clinical diagnosis,treatment planning,and follow-up procedures.Periapical ra-diographic examination and numerous panoramic systems are used in routine clinical dental practice.Cone beam CT is widely used and currently the method of choice in oral and maxillofacial implantology,endodontics,maxillofacial surgery,periodontics,degenerative temporomandibular joint disease,orthodontics,airway studies,sleep disorders,and forensic dentistry.Another innovative laboratory re-search tool that offers three-dimensional(3D)detailed high-resolution images of in vitro teeth and neighboring structures with submicrometric accuracy is micro-computed tomography.Ultra-high radiation doses,long scanning times,and high costs preclude its routine clinical use.In response to the high demand for a te-chnique that could provide real-time images using a cost-effective,rapid,user-friendly,and portable technique without ionizing radiation,some authors pro-posed ultrasound imaging methods as an alternative to X-ray imaging techniques.Ultrasonography can be used in the dentomaxillofacial region for various diagno-stic purposes such as salivary gland and superficial tissue examination.Recently,dedicated dental magnetic resonance imaging with appropriate software,hard-ware,sequences,and field of view tailored to fit dentomaxillofacial anatomy was introduced.Lately,3D printing technologies and their application in dentistry has attracted attention.During 3D printing a given material is added in successive layers to create a 3D object.The application of this technology has the potential to decrease operation time and minimize operator bias and the possibility of proce-dural errors.Another hot topic regarding dentomaxillofacial radiology is artificial intelligence,which is a field related to computer science dedicated to developing systems or machines that can perform tasks traditionally associated with human intelligence.It is obvious that further investigation and research in the field of dentomaxillofacial radiology will make great contributions to diagnostic imaging for various dental specialties.展开更多
Cancer is one of the most important health problems that deeply affects all humanity and will have groundbreaking consequences in human history with its elimination.Gastrointestinal cancers,including colon and rectum,...Cancer is one of the most important health problems that deeply affects all humanity and will have groundbreaking consequences in human history with its elimination.Gastrointestinal cancers,including colon and rectum,stomach,liver,pancreatic,and esophageal,account for 26%of the global cancer incidence and 35%of cancer-related deaths.Unfortunately,it is estimated that today’s high incidence and mortality rates will increase by 58%and 73%by 2040,respectively.Although the treatment process includes novel options such as immunotherapy in addition to classical options with a multidisciplinary approach,surgical treatment under general anesthesia remains the leading option.Considering a long-lasting cancer process,it is quite surprising that a very short-term anesthetic adminis-tration can have various effects on cancer cell behavior.Various anesthetic approaches such as regional blocks used in pain management,the use of ane-sthetic adjuvants such asβ-adrenoceptor antagonists,nonsteroidal anti-inflam-matory drugs,and intravenous lidocaine,and the choice of anesthetic drugs seem to have potential effects on long-term cancer outcomes.Propofol is an intravenous anesthetic drug that is used for both induction and maintenance of general anesthesia.Many in vitro and clinical studies examining the effects of propofol comparatively with other anesthetic agents on tumor recurrence and metastasis revealed possible effects on tumor cell signaling,the immune response,and the modulation of the neuroendocrine stress response.However,the evidence from all these in vitro and clinical studies is different,complicated,and inconsistent.The general effects of propofol on the behavioral patterns,growth,and metastasis of gastrointestinal tumor cells,as well as the clinical features and consequences resulting from these effects,constitute the subject of this review.展开更多
Background: Bisphosphonates are compounds similar to inorganic pyrophosphates, with anti-angiogenic, anti-inflammatory, anti-bone resorption, and antitumor effects. They are used to prevent bone metastases in cancer a...Background: Bisphosphonates are compounds similar to inorganic pyrophosphates, with anti-angiogenic, anti-inflammatory, anti-bone resorption, and antitumor effects. They are used to prevent bone metastases in cancer and treat osteoporosis. However, a major side effect is osteonecrosis of the jaw (ONJ), first reported in 2003, which is the exposure of necrotic bone in the mouth, often accompanied by infection. Prevention and treatment of ONJ remain challenging due to a lack of reliable epidemiological data on its incidence and risk factors. The aim of our work is to estimate the prevalence of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and to analyze its correlations with different risk factors reported in the literature. Methods: A retrospective observational study was conducted in the Oncology, Rheumatology and Maxillofacial Surgery Department of the Mohamed VI University Hospital;Marrakech, based on complete medical records during the period 2017 to 2022, as well as consultations of patients under bisphosphonates in these departments during July and August 2022. Statistical analysis was performed using IBM SPSS version 16.0. Results: Of the 107 patients included in this study, 60.7% were women, and 56.1% were between 40 and 65 years old. 100% of the patients suffered from a general pathology. Regarding treatment with BP, 103 patients were treated with Zoledronate, 4 with Risedronate, and 5 with Ibandronate. 103 patients received this treatment intravenously, while 4 patients received it orally. Regarding Oral health, only 12.1% of patients reported a poor oral health. 99.1% of patients were informed of the need for oral preparation prior to BP treatment, but only 71.02% received dental treatment, mainly tooth extraction. None of the patients were diagnosed with BRONJ. Conclusions: While the global incidence of ONJ ranges from 0.8% to 12%, our finding is zero. Most previous studies are retrospective with limited patient numbers like our study. To accurately assess the prevalence of ONJ, further prospective epidemiological studies with standardized protocols and thorough follow-up over several years are essential.展开更多
OBJECTIVE:To examine the effect of electroacupuncture(EA)application on postoperative pain levels and the amount of analgesic use in patients who underwent periodontal flap surgery.METHODS:In this prospective,randomiz...OBJECTIVE:To examine the effect of electroacupuncture(EA)application on postoperative pain levels and the amount of analgesic use in patients who underwent periodontal flap surgery.METHODS:In this prospective,randomized and controlled study,patients planned for periodontal surgery were divided into two groups[EA group(n=22):patients who received electroacupuncture and control group(n=22):patients who did not receive electroacupuncture].Electroacupuncture was applied bilaterally to the Hegu(LI4),Daying(ST5),and Jiache(ST5)points of the patients in the EA group immediately before and after the flap surgery for 30 min at a frequency of 50 Hz to each point at a current that the patient could tolerate.The patients in the control group underwent flap surgery without applying electroacupuncture.Patients were asked to record their pain levels on the visual analog scale and the number of analgesic tablets they took during the 7 postoperative days on the given form.RESULTS:The total mean score of pain felt in the EA group(16.60±2.78)was found to be significantly lower than that in the control group(31.37±2.78)(P=0.001).No significant difference was found between the groups regarding the amount of analgesics taken(4.77±4.72,5.82±3.11)(P=0.111).CONCLUSIONS:Our study shows that electroacupuncture application is beneficial in reducing pain after periodontal flap surgery.Overall,more research is needed to fully understand the effects of electroacupuncture on postoperative pain.展开更多
BACKGROUND Smoking is a leading cause of carcinogenesis in the head and neck region,representing a critical public health issue.Identifying genotoxic damage in smokers can provide valuable insights for developing prev...BACKGROUND Smoking is a leading cause of carcinogenesis in the head and neck region,representing a critical public health issue.Identifying genotoxic damage in smokers can provide valuable insights for developing preventive interventions.AIM To assess genotoxic damage through the micronucleus assay in exfoliated buccal mucosa cells from users of conventional tobacco,reverse smoking,cannabis,electronic cigarettes,and non-smokers.METHODS A cross-sectional study was conducted with 100 participants divided into five groups:20 conventional tobacco smokers,20 reverse smokers,20 electronic cigarette users,20 cannabis users,and 20 non-smokers.Exfoliated buccal mucosa cells were analyzed using Giemsa and Papanicolaou staining to identify micronuclei(MN)as markers of genotoxic damage.RESULTS MN were present in 86%of the samples.Statistically significant differences were observed in the median micronucleus count between conventional,reverse,and electronic cigarette smokers compared to non-smokers(P<0.001),while no significant difference was found for cannabis smokers(P=0.89).A significant correlation was identified between the presence of oral lesions and micronucleus count(P=0.03).Regression analysis ruled out alcohol as a confounding factor.CONCLUSION This study identified genotoxic damage associated with various smoking habits,except for cannabis use,highlighting the need for public health interventions to reduce smoking and mitigate its genotoxic effects.These findings provide a foundation for future research and the implementation of preventive policies.展开更多
The article by Chauhan et al highlights the transformative potential of magnification tools in improving precision and outcomes across various dental specialties.While the authors discuss the advantages of magnificati...The article by Chauhan et al highlights the transformative potential of magnification tools in improving precision and outcomes across various dental specialties.While the authors discuss the advantages of magnification,they do not address the potential integration of artificial intelligence(AI)with magnification devices to further enhance diagnostic and therapeutic efficiency.This letter explores the synergy of AI with magnification tools,emphasizing its applicability in image-guided diagnostics,workflow optimization,and personalized treatment planning.The integration of AI and magnification also paves the way for personalized,data-driven treatment strategies,marking a significant evolution in dental care.However,it is important to acknowledge the limitations and challenges associated with AI,such as data privacy concerns,algorithmic biases,and the need for robust validation before clinical implementation.This discussion underscores the need for interdisciplinary research to realize this potential.展开更多
Th e perspective article by Yang et al.[1],“Build the virtual cell with artificial intelligence:a perspective for cancer research”,provides a compelling vision for the transformative potential of artificial intellig...Th e perspective article by Yang et al.[1],“Build the virtual cell with artificial intelligence:a perspective for cancer research”,provides a compelling vision for the transformative potential of artificial intelligence virtual cells(AIVCs)in oncology.The authors outline how AIVCs could revolutionize cancer research by enabling in silico experimentation,overcoming multiomics bottlenecks,and accelerating drug development.However,the article presents a comprehensive framework,yet several pivotal questions remain unresolved,which warrant further discussion to advance the fi eld.展开更多
BACKGROUND Minimally invasive lumbar interbody fusion(LIF)procedures have evolved rapidly in recent years,with robot-assisted(RA)techniques increasingly integrated into clinical practice.However,questions remain regar...BACKGROUND Minimally invasive lumbar interbody fusion(LIF)procedures have evolved rapidly in recent years,with robot-assisted(RA)techniques increasingly integrated into clinical practice.However,questions remain regarding the relative advantages of RA over traditional fluoroscopy-guided and navigation-assisted methods in terms of perioperative,radiographic,and clinical outcomes.This systematic review synthesizes current evidence on these comparisons,focusing on the accuracy of screw placement,perioperative efficiency,radiographic and clinical outcomes,and complications.AIM To investigate the comparative effectiveness of RA vs conventional LIF techniques.METHODS A systematic review and meta-analysis was conducted in accordance with PRISMA 2020 and Cochrane guidelines.Databases searched included PubMed,EMBASE,Web of Science,Scopus,and the Cochrane Library(through May 2025).Eligible studies were randomized controlled trials and observational studies comparing RA with fluoroscopy-or navigation-guided LIF(transforaminal lumbar interbody fusion,lateral lumbar interbody fusion,oblique lumbar interbody fusion)in adults.Two reviewers independently extracted data and assessed risk of bias.The Grading of Recommendations Assessment,Development and Evaluation framework was used to evaluate certainty of evidence.Meta-analyses were performed where data were sufficiently homogeneous.RESULTS Twenty-two studies were included,encompassing a total of 2313 patients-1046 who underwent RA-guided procedures and 1267 who received comparator techniques.Meta-analyses showed that RA significantly improved perfect pedicle screw placement[pooled odds ratio=2.93;95%confidence interval(CI):1.40-6.14;I2=78.2%]and reduced intraoperative blood loss(pooled standardized mean difference=-0.28;95%CI:-0.47 to-0.08;I2=0%).Operative time did not significantly differ between groups(pooled standardized mean difference=0.01;95%CI:-0.30 to 0.31;I2=66%).Radiation dose could not be synthesized quantitatively due to heterogeneous definitions and measurement units.Narratively,RA demonstrated consistent advantages in reducing surgical exposure and adjacent segment degeneration.Clinical and radiographic outcomes,fusion success,and complication rates were generally comparable across groups.CONCLUSION RA LIF improves pedicle screw placement accuracy and reduces blood loss and surgeon radiation exposure while maintaining similar clinical outcomes and safety profiles to conventional techniques.These findings support the integration of RA into spine surgery but highlight the need for high-quality multicenter randomized controlled trials and cost-effectiveness studies to guide broader implementation.展开更多
BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves ...BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves mechanical debridement and plaque control,but localized antimicrobial therapy offers site-specific advantages.While antibiotics such as metronidazole and doxycycline are commonly used,green tea extract,which is rich in epigallocatechin gallate,has been proposed as a promising alternative for local drug delivery due to its anti-inflammatory and antimicrobial properties.AIM To compare the clinical efficacy of green tea extract gel and ornidazole gel as adjuncts to scaling and root planing(SRP)in patients with periodontitis.METHODS Teeth with probing pocket depths(PPD)of 4-7 mm were selected.Participants’baseline oral hygiene index-simplified,plaque index,clinical attachment loss,and PPD were recorded.The participants were randomized into two groups:One received green tea extract gel after SRP,and the other received ornidazole gel.Subgingival drug delivery was performed,and participants refrained from brushing or interproximal cleaning for ten days.Their clinical parameters were re-evaluated after one month.RESULTS The PPD decreased significantly from baseline to one month in both groups.However,the green tea extract gel group exhibited superior outcomes to the ornidazole gel group,with a mean difference in PPD of 0.28±0.78 mm at one month(P<0.007).CONCLUSION As an adjunct to SRP,green tea extract gel showed greater efficacy in improving clinical periodontal parameters than ornidazole gel.展开更多
Objective:To assess the burden of Group B Streptococcus(GBS)and analyze the distribution of scrotypes in relation to their source.The review highlights data gaps in transmission dynamics and regional food consumption ...Objective:To assess the burden of Group B Streptococcus(GBS)and analyze the distribution of scrotypes in relation to their source.The review highlights data gaps in transmission dynamics and regional food consumption practices,which are esscntial for designing cffective public health strategies and advancing vaccine development.Methods:Scarches were conducted in Web of Science,MEDLINE,Science Direct,PubMcd,and Scopus databases to find studies related to GBS during 1990-2025.Eligible studies were those that described prevalence,scrotype distribution or scquence type(ST)of GBS in Southeast Asian countries.Random-cffects meta-analysis was used to pool data.Results:A total of 26 studies met the inclusion criteria from cight countries.The pooled estimate of maternal GBS colonization was 15.1%,with scrotypesII,v,ⅡI,V,and I a accounting for the majority of cases(91.24%)in the Southcast Asia studies.Data on ST was limited;however,ST1 was found to be predominant in Malaysia and Thailand,while ST283 was notably linked to the consumption of raw fish.Conclusions:The pooled estimate of the maternal colonization with GBS was 15.1%which is cquivalent to many other primary and review reports worldwide.Distribution of scrotype and ST is needed to be studied in Southcast Asian countries to devise cffective preventive measures.These findings underscore thc importance of surveillance and tailored prevention strategies to combat GBS infections in Southcast Asia.展开更多
BACKGROUND Colorectal cancer is the third most common malignancy globally,with the liver being the predominant site of metastatic disease.AIM To evaluate safety,feasibility,and outcomes of robotic liver resection(RLR)...BACKGROUND Colorectal cancer is the third most common malignancy globally,with the liver being the predominant site of metastatic disease.AIM To evaluate safety,feasibility,and outcomes of robotic liver resection(RLR)versus laparoscopic liver resection(LLR)and open liver resection(OLR)for colorectal metastasis(CRLM).METHODS This study followed Preferred Reporting Items for Systematic reviews and Meta-Analyses guidelines.Systematic searches in PubMed,EMBASE,Scopus,and Cochrane Library identified comparative and noncomparative reviews evaluating RLR versus LLR or OLR for CRLM.Two independent reviewers screened studies using predefined PICO(Population,Intervention,Comparator,Outcome)criteria,with data extraction focusing on conversion rates,operative outcomes,morbidity,mortality,and survival.Methodological quality was assessed via Assessment of Multiple Systematic Reviews 2.Pooled analyses were performed for comparative data;noncomparative studies were narratively synthesized.RESULTS Pooled evidence from two comparative systematic reviews(9792 patients)demonstrated that RLR offers distinct advantages over LLR and OLR,including significantly lower conversion rates(4.7%–6.7%vs 10.4%–12.4%,P<0.001)and reduced intraoperative blood loss(190.8–266.8 mL vs 283.9–294.3 mL,P<0.001)despite longer operating times(mean 304.1 vs 191.8 min).Perioperative safety and oncologic outcomes(R0 resection>82%;5-year overall survival:53.1%–60.8%)were comparable across approaches.Three additional noncomparative reviews(n=274)highlighted the technical practicability of RLR in complex cases(zero conversions in small cohorts,median 399.5 min for simultaneous resections).However,these findings were not included in pooled analyses due to the lack of comparator groups.Noncomparative data(n=274)revealed higher upfront costs for RLR due to prolonged operating times(median 399.5 min)and the need for expensive equipment;however,no formal cost comparisons were available.CONCLUSION RLR is a safe and feasible alternative to LLR and OLR for CRLM,demonstrating superior technical performance and comparable short-term outcomes.展开更多
Earthworms play a vital role in enhancing soil quality and structure in agricultural ecosystems.This study investigated the diversity of earthworm species found in banana and guava orchards in Nakhon Pathom,Thailand,w...Earthworms play a vital role in enhancing soil quality and structure in agricultural ecosystems.This study investigated the diversity of earthworm species found in banana and guava orchards in Nakhon Pathom,Thailand,where 166 samples were collected.The results showed that 56.63%of earthworms were in the adult stage.In the banana orchard,researchers identified two families and four species:from the family Megascolecidae,they found Amynthas alexandri,Metaphire posthuma,and Polypheretima elongata,along with one species from the family Moniligastridae,Drawida sp.In the guava orchard,two families and two species were identified:from the family Megascolecidae,Metaphire posthuma,and from the family Moniligastridae,Drawida sp.Fungi isolated from the intestines of earthworms,precisely the strains EW2,EW3,EW6,EW13,EW16,EW23,EW25,EW26,EW28,EW38,EW39,EW40,EW41,EW43,and EW44,have demonstrated the ability to produce cellulase.Among these,the fungus EW41 exhibited the highest cellulase activity,measuring 32.97 units per milliliter at an optimal temperature of 60℃ and a pH of 5.0.This study high-ranlightsthat most earthworm species in agricultural soils belong to the family Megascolecidae.Furthermore,earthwormsplay a crucial role in enhancing the chemical properties of the soil.The cellulolytic fungi present in earthworm intestinescontribute to the natural decomposition of organic matter,thereby promoting soil health by converting plant residuesinto nutrient-rich compost.These findings suggest promising applications of earthworm-associated fungi in sustainableagriculture and organic waste management.展开更多
This study investigates the diversity of gut microbiota in Metaphire peguana,an earthworm species commonly found in agricultural areas of Thailand.Earthworms play a critical role in soil ecosystems by supporting nutri...This study investigates the diversity of gut microbiota in Metaphire peguana,an earthworm species commonly found in agricultural areas of Thailand.Earthworms play a critical role in soil ecosystems by supporting nutrient cycling and breaking down organic matter.Understanding the microbial diversity in their gut is essential for exploring their ecological contributions.Using Next Generation Sequencing(NGS),we analyzed the mycobiome in the gut of M.peguana.Our findings revealed a high diversity of fungal species,primarily belonging to two major phyla:Ascomycota and Basidiomycota.Ascomycota was the most abundant phylum,comprising 40.1% of the total fungal species identified.A total of 33 distinct fungal species were identified,which underscores the richness of microbial life within the earthworm gut.This study successfully created the first genetic database of the microbial community in M.peguana,providing a foundation for future research in agricultural applications.The microbial species identified,particularly siderophoreproducing fungi,could have significant implications for improving soil fertility and promoting sustainable agricultural practices.The use of NGS technology has enabled comprehensive profiling of microbial communities,allowing for precise identification of fungi that may play essential roles in soil health.Furthermore,the study paves the way for future studies on the potential applications of earthworm gut microbiomes in biotechnology,especially in enhancing soil nutrient availability and plant growth.The findings of this research contribute to the broader understanding of the ecological roles of earthworms and their microbiomes in soil ecosystems.展开更多
文摘BACKGROUND Gamification,the use of game materials in education,has been used in several scientific fields,and studies have demonstrated its effectiveness in student comprehension.AIM To evaluate whether the success of 4th-and 5th-grade dentistry students increases when using the gamification applications Kahoot!and Mentimeter.METHODS An experimental design was used to measure the effect of gamification applications on students who were taught using the traditional method.For this purpose,Mentimeter and Kahoot!applications were used in this experimental study.Three groups of 4th-and 5th-grade students were formed:Mentimeter,Kahoot!,and control groups.The effect of gamification applications on achievement was evaluated by administering a post-test to these groups after the instructional process.One-way analysis of variance and t-tests were used for statistical analyses.RESULTS A total of 35.5%of the students were in the Mentimeter group,29.8%in the Kahoot!group,and 34.6%in the control group.Of the total number of students,54.8%were female and 45.2%were male,while 52.2%were 5th-grade students and 47.8%were 4th-grade students.The overall grade point average was found to be 66.65(range,24-100).There was a statistically significant difference in average success scores between students of different groups(P=0.003<0.05)and grades(P=0.036<0.05).The average success score was the highest in the Mentimeter group(71.64)and the lowest in the Kahoot!group(62.15).In the control group,this score was 65.41.The average success score was higher for students in the 5thgrade(68.16)and for female students(68.84).CONCLUSION Gamification has a great potential in the education of dentomaxillofacial radiology students.Mentimeter outperformed both the Kahoot!and the traditional method in terms of average student success rates.
文摘BACKGROUND Robotic assistance is increasingly used for donor and recipient hepatectomy in liver transplantation,yet existing evidence is fragmented and variably indirect.AIM To evaluate clinical outcomes,surgical performance,and economic effects of robotic-assisted donor and recipient hepatectomy in the transplant pathway.METHODS Following Preferred Reporting Items for Systematic reviews and Meta-Analyses 2020 and a priori registration,systematic reviews were included with or without meta-analysis.Four databases were searched through July 2025.Methodological quality was appraised with a measurement tool to assess systematic reviews(AMSTAR 2),and certainty was graded with grading of recommendations assessment,development and evaluation(GRADE).Evidence overlap was calculated via a citation-matrix-based corrected covered area(CCA).Effect sizes were prespecified as risk ratios(RR)for dichotomous outcomes and mean differences for continuous outcomes.RESULTS Five reviews met the inclusion criteria,four with meta-analyses and one consensus review used only for context.Donor(direct)findings were more favorable for robotics in terms of estimated blood loss(≈-117 mL)and length of stay(≈-0.6 days),although with longer operative time(≈+105 minutes).Absolute risks for donor complications were not estimable from ratio-only data.Recipient(indirect)meta-analysis indicated robotics to be favorable in terms of conversion(RR≈0.41)and severe morbidity(RR≈0.81),with a trend toward lower overall morbidity(RR≈0.92)and no difference in 30-day mortality.Differences in length of stay and operative time were small and heterogeneous.Economic evidence(indirect,network meta-analysis)suggested higher procedural costs for robotic vs laparoscopic intervention,but lower hospitalization costs vs open intervention,with laparoscopy the least expensive overall.AMSTAR 2 ratings were moderate-to-high across the reviews,GRADE certainty was low for key donor continuous outcomes,and low-to-moderate for recipient and economic outcomes.Overlap was slight(graded-corpus CCA=0.0%;including a contextual non-transplant review increased CCA to≈1.25%).CONCLUSION Robotic donor hepatectomy confers perioperative advantages at the cost of longer operative time.Recipient and economic findings are indirect and considered hypothesis-generating.Transplant-specific,prospective comparisons using a minimum standardized dataset and uniform outcome definitions are needed to resolve remaining uncertainties and to clarify the cost-utility correlation.
文摘Cone-beam computed tomography(CBCT) was developed and introduced specifically for dento-maxillofacial imaging. CBCT possesses a number of advantages over medical CT in clinical practice, such as lower effective radiation doses, lower costs, fewer space requirements,easier image acquisition, and interactive display modes such as mutiplanar reconstruction that are applicable to maxillofacial imaging. However, the disadvantages of CBCT include higher doses than two-dimensional imaging; the inability to accurately represent the internal structure of soft tissues and soft-tissue lesions; a limited correlation with Hounsfield Units for standardized quantification of bone density; and the presence of various types of image artifacts, mainly those produced by metal restorations. CBCT is now commonly used for a variety of purposes in oral implantology, dentomaxillofacial surgery, image-guided surgical procedures, endodontics, periodontics and orthodontics. CBCT applications provide obvious benefits in the assessment of dentomaxillofacial region, however; it should be used only in correct indications considering the necessity and the potential hazards of the examination.
文摘BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors is their penetration of neighboring tissues,such as lymphatic and blood arteries,due to the tumor cells'capacity to break down the extracellular matrix(ECM).Matrix metalloproteinases(MMPs)constitute a family of proteolytic enzymes that facilitate tissue remodeling and the degradation of the ECM.MMP-9 and MMP-13 belong to the group of extracellular matrix degrading enzymes and their expression has been studied in OSCC because of their specific functions.MMP-13,a collagenase family member,is thought to play an essential role in the MMP activation cascade by breaking down the fibrillar collagens,whereas MMP-9 is thought to accelerate the growth of tumors.Elevated MMP-13 expression has been associated with tumor behavior and patient prognosis in a number of malignant cases.AIM To assess the immunohistochemical expression of MMP-9 and MMP-13 in OSCC.METHODS A total of 40 cases with histologically confirmed OSCC by incisional biopsy were included in this cross-sectional retrospective study.The protocols for both MMP-9 and MMP-13 immunohistochemical staining were performed according to the manufacturer’s recommendations along with the normal gingival epithelium as a positive control.All the observations were recorded and Pearson’sχ²test with Fisher exact test was used for statistical analysis.RESULTS Our study showed no significant correlation between MMP-9 and MMP-13 staining intensity and tumor size.The majority of the patients were in advanced TNM stages(III and IV),and showed intense expression of MMP-9 and MMP-13.CONCLUSION The present study suggests that both MMP-9 and MMP-13 play an important and independent role in OSCC progression and invasiveness.Intense expression of MMP-9 and MMP-13,irrespective of histological grade of OSCC,correlates well with TNM stage.Consequently,it is evident that MMP-9 and MMP-13 are important for the invasiveness and progression of tumors.The findings may facilitate the development of new approaches for evaluating lymph node metastases and interventional therapy techniques,hence enhancing the prognosis of patients diagnosed with OSCC.
文摘The Ottoman-Turkish medicine and dentistry was born and developed in the lands of Anatolia. The most mature period of the Islamic medicine was in the Ottoman Empire on the lands of Anatolia. While inheriting the Turkish-Islamic science and culture structure and the ancient age and Old Greek science, it was integrated with the medicine philosophy here. In the 13th and 15th centuries, in Ottomans, the development of the dentistry was on the issues of the dental diseases and periodontology. In the 14th and 15th centuries, it was observed in the medicine books of the Turkish physician writers that they were also dealing with the child dentistry as issues. That the dental medicine took place in the works of art written between the 15th and 19th century shows the existence of the surgeons realizing the tooth extraction. With the opening of the first dentist school in the year of 1909, the training of dentistry started. In this study, the issue of the development and training of the dentistry in the Ottomans among the 13th-19th centuries were dealt with the outlines. Also, the first examples and fundamental works of art of the dentistry literature from the Ottoman Empire to the Republican period will be told shortly.
文摘Epilepsy has direct negative effects on sufferers’ general dental condition and oral health, both of which are further affected by inadequate oral hygiene;poor oral hygiene itself is often also caused by epilepsy-related poor health. Consequently, tooth loss, caries and periodontal disease occur increasingly often in epilepsy sufferers and they need more dental treatment. However, in fact the epileptic patients can receive fewer and simpler treatment modalities. The aim of this study was to review and synthesize recent studies on dental treatment in epilepsy patients and to mention potential triggers for seizures in dental practice.
文摘BACKGROUND In the absence of effective antimicrobials,transplant surgery is not viable,and antirejection immunosuppressants cannot be administered,as resistant infections compromise the life-saving goal of organ transplantation.AIM To evaluate the efficacy of antimicrobials in preventing resistance in solid organ transplant recipients.METHODS A systematic review was conducted using a search methodology consistent with the preferred reporting items for systematic reviews and meta-analyses.This review included randomized clinical trials that evaluated the efficacy of antimicrobial agents(prophylactic or therapeutic)aimed at preventing antimicrobial resistance.The search strategy involved analyzing multiple databases,including PubMed/MEDLINE,Web of Science,Embase,Scopus,and SciELO,as well as examining gray literature sources on Google Scholar.A comprehensive electronic database search was conducted from the databases’inception until May 2024,with no language restrictions.RESULTS After the final phase of the eligibility assessment,this systematic review ultimate-ly included 7 articles.A total of 2318 patients were studied.The most studied microorganisms were cytomegalovirus,although vancomycinresistant enterococci,Clostridioides difficile,and multidrug-resistant Enterobacterales were also analyzed.The antimicrobials used in the interventions were mainly maribavir,valganciclovir,gancic-lovir,and colistin-neomycin.Of concern,all clinical trials showed significant proportions of resistant microorga-nisms after the interventions,with no statistically significant differences between the groups(mean resistance 13.47%vs 14.39%),except for two studies that demonstrated greater efficacy of maribavir and valganciclovir(mean resistance 22.2%vs 41.1%in the control group;P<0.05).The total reported deaths in three clinical trials were 75,and there were 24 graft rejections in two studies.CONCLUSION All clinical trials reported significant proportions of antimicrobial-resistant microorganisms following interventions.More high-quality randomized clinical trials are needed to corroborate these results.
文摘Oral and maxillofacial diagnostic imaging is of paramount importance in dental clinical diagnosis,treatment planning,and follow-up procedures.Periapical ra-diographic examination and numerous panoramic systems are used in routine clinical dental practice.Cone beam CT is widely used and currently the method of choice in oral and maxillofacial implantology,endodontics,maxillofacial surgery,periodontics,degenerative temporomandibular joint disease,orthodontics,airway studies,sleep disorders,and forensic dentistry.Another innovative laboratory re-search tool that offers three-dimensional(3D)detailed high-resolution images of in vitro teeth and neighboring structures with submicrometric accuracy is micro-computed tomography.Ultra-high radiation doses,long scanning times,and high costs preclude its routine clinical use.In response to the high demand for a te-chnique that could provide real-time images using a cost-effective,rapid,user-friendly,and portable technique without ionizing radiation,some authors pro-posed ultrasound imaging methods as an alternative to X-ray imaging techniques.Ultrasonography can be used in the dentomaxillofacial region for various diagno-stic purposes such as salivary gland and superficial tissue examination.Recently,dedicated dental magnetic resonance imaging with appropriate software,hard-ware,sequences,and field of view tailored to fit dentomaxillofacial anatomy was introduced.Lately,3D printing technologies and their application in dentistry has attracted attention.During 3D printing a given material is added in successive layers to create a 3D object.The application of this technology has the potential to decrease operation time and minimize operator bias and the possibility of proce-dural errors.Another hot topic regarding dentomaxillofacial radiology is artificial intelligence,which is a field related to computer science dedicated to developing systems or machines that can perform tasks traditionally associated with human intelligence.It is obvious that further investigation and research in the field of dentomaxillofacial radiology will make great contributions to diagnostic imaging for various dental specialties.
文摘Cancer is one of the most important health problems that deeply affects all humanity and will have groundbreaking consequences in human history with its elimination.Gastrointestinal cancers,including colon and rectum,stomach,liver,pancreatic,and esophageal,account for 26%of the global cancer incidence and 35%of cancer-related deaths.Unfortunately,it is estimated that today’s high incidence and mortality rates will increase by 58%and 73%by 2040,respectively.Although the treatment process includes novel options such as immunotherapy in addition to classical options with a multidisciplinary approach,surgical treatment under general anesthesia remains the leading option.Considering a long-lasting cancer process,it is quite surprising that a very short-term anesthetic adminis-tration can have various effects on cancer cell behavior.Various anesthetic approaches such as regional blocks used in pain management,the use of ane-sthetic adjuvants such asβ-adrenoceptor antagonists,nonsteroidal anti-inflam-matory drugs,and intravenous lidocaine,and the choice of anesthetic drugs seem to have potential effects on long-term cancer outcomes.Propofol is an intravenous anesthetic drug that is used for both induction and maintenance of general anesthesia.Many in vitro and clinical studies examining the effects of propofol comparatively with other anesthetic agents on tumor recurrence and metastasis revealed possible effects on tumor cell signaling,the immune response,and the modulation of the neuroendocrine stress response.However,the evidence from all these in vitro and clinical studies is different,complicated,and inconsistent.The general effects of propofol on the behavioral patterns,growth,and metastasis of gastrointestinal tumor cells,as well as the clinical features and consequences resulting from these effects,constitute the subject of this review.
文摘Background: Bisphosphonates are compounds similar to inorganic pyrophosphates, with anti-angiogenic, anti-inflammatory, anti-bone resorption, and antitumor effects. They are used to prevent bone metastases in cancer and treat osteoporosis. However, a major side effect is osteonecrosis of the jaw (ONJ), first reported in 2003, which is the exposure of necrotic bone in the mouth, often accompanied by infection. Prevention and treatment of ONJ remain challenging due to a lack of reliable epidemiological data on its incidence and risk factors. The aim of our work is to estimate the prevalence of bisphosphonate-related osteonecrosis of the jaw (BRONJ) and to analyze its correlations with different risk factors reported in the literature. Methods: A retrospective observational study was conducted in the Oncology, Rheumatology and Maxillofacial Surgery Department of the Mohamed VI University Hospital;Marrakech, based on complete medical records during the period 2017 to 2022, as well as consultations of patients under bisphosphonates in these departments during July and August 2022. Statistical analysis was performed using IBM SPSS version 16.0. Results: Of the 107 patients included in this study, 60.7% were women, and 56.1% were between 40 and 65 years old. 100% of the patients suffered from a general pathology. Regarding treatment with BP, 103 patients were treated with Zoledronate, 4 with Risedronate, and 5 with Ibandronate. 103 patients received this treatment intravenously, while 4 patients received it orally. Regarding Oral health, only 12.1% of patients reported a poor oral health. 99.1% of patients were informed of the need for oral preparation prior to BP treatment, but only 71.02% received dental treatment, mainly tooth extraction. None of the patients were diagnosed with BRONJ. Conclusions: While the global incidence of ONJ ranges from 0.8% to 12%, our finding is zero. Most previous studies are retrospective with limited patient numbers like our study. To accurately assess the prevalence of ONJ, further prospective epidemiological studies with standardized protocols and thorough follow-up over several years are essential.
文摘OBJECTIVE:To examine the effect of electroacupuncture(EA)application on postoperative pain levels and the amount of analgesic use in patients who underwent periodontal flap surgery.METHODS:In this prospective,randomized and controlled study,patients planned for periodontal surgery were divided into two groups[EA group(n=22):patients who received electroacupuncture and control group(n=22):patients who did not receive electroacupuncture].Electroacupuncture was applied bilaterally to the Hegu(LI4),Daying(ST5),and Jiache(ST5)points of the patients in the EA group immediately before and after the flap surgery for 30 min at a frequency of 50 Hz to each point at a current that the patient could tolerate.The patients in the control group underwent flap surgery without applying electroacupuncture.Patients were asked to record their pain levels on the visual analog scale and the number of analgesic tablets they took during the 7 postoperative days on the given form.RESULTS:The total mean score of pain felt in the EA group(16.60±2.78)was found to be significantly lower than that in the control group(31.37±2.78)(P=0.001).No significant difference was found between the groups regarding the amount of analgesics taken(4.77±4.72,5.82±3.11)(P=0.111).CONCLUSIONS:Our study shows that electroacupuncture application is beneficial in reducing pain after periodontal flap surgery.Overall,more research is needed to fully understand the effects of electroacupuncture on postoperative pain.
文摘BACKGROUND Smoking is a leading cause of carcinogenesis in the head and neck region,representing a critical public health issue.Identifying genotoxic damage in smokers can provide valuable insights for developing preventive interventions.AIM To assess genotoxic damage through the micronucleus assay in exfoliated buccal mucosa cells from users of conventional tobacco,reverse smoking,cannabis,electronic cigarettes,and non-smokers.METHODS A cross-sectional study was conducted with 100 participants divided into five groups:20 conventional tobacco smokers,20 reverse smokers,20 electronic cigarette users,20 cannabis users,and 20 non-smokers.Exfoliated buccal mucosa cells were analyzed using Giemsa and Papanicolaou staining to identify micronuclei(MN)as markers of genotoxic damage.RESULTS MN were present in 86%of the samples.Statistically significant differences were observed in the median micronucleus count between conventional,reverse,and electronic cigarette smokers compared to non-smokers(P<0.001),while no significant difference was found for cannabis smokers(P=0.89).A significant correlation was identified between the presence of oral lesions and micronucleus count(P=0.03).Regression analysis ruled out alcohol as a confounding factor.CONCLUSION This study identified genotoxic damage associated with various smoking habits,except for cannabis use,highlighting the need for public health interventions to reduce smoking and mitigate its genotoxic effects.These findings provide a foundation for future research and the implementation of preventive policies.
文摘The article by Chauhan et al highlights the transformative potential of magnification tools in improving precision and outcomes across various dental specialties.While the authors discuss the advantages of magnification,they do not address the potential integration of artificial intelligence(AI)with magnification devices to further enhance diagnostic and therapeutic efficiency.This letter explores the synergy of AI with magnification tools,emphasizing its applicability in image-guided diagnostics,workflow optimization,and personalized treatment planning.The integration of AI and magnification also paves the way for personalized,data-driven treatment strategies,marking a significant evolution in dental care.However,it is important to acknowledge the limitations and challenges associated with AI,such as data privacy concerns,algorithmic biases,and the need for robust validation before clinical implementation.This discussion underscores the need for interdisciplinary research to realize this potential.
文摘Th e perspective article by Yang et al.[1],“Build the virtual cell with artificial intelligence:a perspective for cancer research”,provides a compelling vision for the transformative potential of artificial intelligence virtual cells(AIVCs)in oncology.The authors outline how AIVCs could revolutionize cancer research by enabling in silico experimentation,overcoming multiomics bottlenecks,and accelerating drug development.However,the article presents a comprehensive framework,yet several pivotal questions remain unresolved,which warrant further discussion to advance the fi eld.
文摘BACKGROUND Minimally invasive lumbar interbody fusion(LIF)procedures have evolved rapidly in recent years,with robot-assisted(RA)techniques increasingly integrated into clinical practice.However,questions remain regarding the relative advantages of RA over traditional fluoroscopy-guided and navigation-assisted methods in terms of perioperative,radiographic,and clinical outcomes.This systematic review synthesizes current evidence on these comparisons,focusing on the accuracy of screw placement,perioperative efficiency,radiographic and clinical outcomes,and complications.AIM To investigate the comparative effectiveness of RA vs conventional LIF techniques.METHODS A systematic review and meta-analysis was conducted in accordance with PRISMA 2020 and Cochrane guidelines.Databases searched included PubMed,EMBASE,Web of Science,Scopus,and the Cochrane Library(through May 2025).Eligible studies were randomized controlled trials and observational studies comparing RA with fluoroscopy-or navigation-guided LIF(transforaminal lumbar interbody fusion,lateral lumbar interbody fusion,oblique lumbar interbody fusion)in adults.Two reviewers independently extracted data and assessed risk of bias.The Grading of Recommendations Assessment,Development and Evaluation framework was used to evaluate certainty of evidence.Meta-analyses were performed where data were sufficiently homogeneous.RESULTS Twenty-two studies were included,encompassing a total of 2313 patients-1046 who underwent RA-guided procedures and 1267 who received comparator techniques.Meta-analyses showed that RA significantly improved perfect pedicle screw placement[pooled odds ratio=2.93;95%confidence interval(CI):1.40-6.14;I2=78.2%]and reduced intraoperative blood loss(pooled standardized mean difference=-0.28;95%CI:-0.47 to-0.08;I2=0%).Operative time did not significantly differ between groups(pooled standardized mean difference=0.01;95%CI:-0.30 to 0.31;I2=66%).Radiation dose could not be synthesized quantitatively due to heterogeneous definitions and measurement units.Narratively,RA demonstrated consistent advantages in reducing surgical exposure and adjacent segment degeneration.Clinical and radiographic outcomes,fusion success,and complication rates were generally comparable across groups.CONCLUSION RA LIF improves pedicle screw placement accuracy and reduces blood loss and surgeon radiation exposure while maintaining similar clinical outcomes and safety profiles to conventional techniques.These findings support the integration of RA into spine surgery but highlight the need for high-quality multicenter randomized controlled trials and cost-effectiveness studies to guide broader implementation.
文摘BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves mechanical debridement and plaque control,but localized antimicrobial therapy offers site-specific advantages.While antibiotics such as metronidazole and doxycycline are commonly used,green tea extract,which is rich in epigallocatechin gallate,has been proposed as a promising alternative for local drug delivery due to its anti-inflammatory and antimicrobial properties.AIM To compare the clinical efficacy of green tea extract gel and ornidazole gel as adjuncts to scaling and root planing(SRP)in patients with periodontitis.METHODS Teeth with probing pocket depths(PPD)of 4-7 mm were selected.Participants’baseline oral hygiene index-simplified,plaque index,clinical attachment loss,and PPD were recorded.The participants were randomized into two groups:One received green tea extract gel after SRP,and the other received ornidazole gel.Subgingival drug delivery was performed,and participants refrained from brushing or interproximal cleaning for ten days.Their clinical parameters were re-evaluated after one month.RESULTS The PPD decreased significantly from baseline to one month in both groups.However,the green tea extract gel group exhibited superior outcomes to the ornidazole gel group,with a mean difference in PPD of 0.28±0.78 mm at one month(P<0.007).CONCLUSION As an adjunct to SRP,green tea extract gel showed greater efficacy in improving clinical periodontal parameters than ornidazole gel.
文摘Objective:To assess the burden of Group B Streptococcus(GBS)and analyze the distribution of scrotypes in relation to their source.The review highlights data gaps in transmission dynamics and regional food consumption practices,which are esscntial for designing cffective public health strategies and advancing vaccine development.Methods:Scarches were conducted in Web of Science,MEDLINE,Science Direct,PubMcd,and Scopus databases to find studies related to GBS during 1990-2025.Eligible studies were those that described prevalence,scrotype distribution or scquence type(ST)of GBS in Southeast Asian countries.Random-cffects meta-analysis was used to pool data.Results:A total of 26 studies met the inclusion criteria from cight countries.The pooled estimate of maternal GBS colonization was 15.1%,with scrotypesII,v,ⅡI,V,and I a accounting for the majority of cases(91.24%)in the Southcast Asia studies.Data on ST was limited;however,ST1 was found to be predominant in Malaysia and Thailand,while ST283 was notably linked to the consumption of raw fish.Conclusions:The pooled estimate of the maternal colonization with GBS was 15.1%which is cquivalent to many other primary and review reports worldwide.Distribution of scrotype and ST is needed to be studied in Southcast Asian countries to devise cffective preventive measures.These findings underscore thc importance of surveillance and tailored prevention strategies to combat GBS infections in Southcast Asia.
文摘BACKGROUND Colorectal cancer is the third most common malignancy globally,with the liver being the predominant site of metastatic disease.AIM To evaluate safety,feasibility,and outcomes of robotic liver resection(RLR)versus laparoscopic liver resection(LLR)and open liver resection(OLR)for colorectal metastasis(CRLM).METHODS This study followed Preferred Reporting Items for Systematic reviews and Meta-Analyses guidelines.Systematic searches in PubMed,EMBASE,Scopus,and Cochrane Library identified comparative and noncomparative reviews evaluating RLR versus LLR or OLR for CRLM.Two independent reviewers screened studies using predefined PICO(Population,Intervention,Comparator,Outcome)criteria,with data extraction focusing on conversion rates,operative outcomes,morbidity,mortality,and survival.Methodological quality was assessed via Assessment of Multiple Systematic Reviews 2.Pooled analyses were performed for comparative data;noncomparative studies were narratively synthesized.RESULTS Pooled evidence from two comparative systematic reviews(9792 patients)demonstrated that RLR offers distinct advantages over LLR and OLR,including significantly lower conversion rates(4.7%–6.7%vs 10.4%–12.4%,P<0.001)and reduced intraoperative blood loss(190.8–266.8 mL vs 283.9–294.3 mL,P<0.001)despite longer operating times(mean 304.1 vs 191.8 min).Perioperative safety and oncologic outcomes(R0 resection>82%;5-year overall survival:53.1%–60.8%)were comparable across approaches.Three additional noncomparative reviews(n=274)highlighted the technical practicability of RLR in complex cases(zero conversions in small cohorts,median 399.5 min for simultaneous resections).However,these findings were not included in pooled analyses due to the lack of comparator groups.Noncomparative data(n=274)revealed higher upfront costs for RLR due to prolonged operating times(median 399.5 min)and the need for expensive equipment;however,no formal cost comparisons were available.CONCLUSION RLR is a safe and feasible alternative to LLR and OLR for CRLM,demonstrating superior technical performance and comparable short-term outcomes.
文摘Earthworms play a vital role in enhancing soil quality and structure in agricultural ecosystems.This study investigated the diversity of earthworm species found in banana and guava orchards in Nakhon Pathom,Thailand,where 166 samples were collected.The results showed that 56.63%of earthworms were in the adult stage.In the banana orchard,researchers identified two families and four species:from the family Megascolecidae,they found Amynthas alexandri,Metaphire posthuma,and Polypheretima elongata,along with one species from the family Moniligastridae,Drawida sp.In the guava orchard,two families and two species were identified:from the family Megascolecidae,Metaphire posthuma,and from the family Moniligastridae,Drawida sp.Fungi isolated from the intestines of earthworms,precisely the strains EW2,EW3,EW6,EW13,EW16,EW23,EW25,EW26,EW28,EW38,EW39,EW40,EW41,EW43,and EW44,have demonstrated the ability to produce cellulase.Among these,the fungus EW41 exhibited the highest cellulase activity,measuring 32.97 units per milliliter at an optimal temperature of 60℃ and a pH of 5.0.This study high-ranlightsthat most earthworm species in agricultural soils belong to the family Megascolecidae.Furthermore,earthwormsplay a crucial role in enhancing the chemical properties of the soil.The cellulolytic fungi present in earthworm intestinescontribute to the natural decomposition of organic matter,thereby promoting soil health by converting plant residuesinto nutrient-rich compost.These findings suggest promising applications of earthworm-associated fungi in sustainableagriculture and organic waste management.
文摘This study investigates the diversity of gut microbiota in Metaphire peguana,an earthworm species commonly found in agricultural areas of Thailand.Earthworms play a critical role in soil ecosystems by supporting nutrient cycling and breaking down organic matter.Understanding the microbial diversity in their gut is essential for exploring their ecological contributions.Using Next Generation Sequencing(NGS),we analyzed the mycobiome in the gut of M.peguana.Our findings revealed a high diversity of fungal species,primarily belonging to two major phyla:Ascomycota and Basidiomycota.Ascomycota was the most abundant phylum,comprising 40.1% of the total fungal species identified.A total of 33 distinct fungal species were identified,which underscores the richness of microbial life within the earthworm gut.This study successfully created the first genetic database of the microbial community in M.peguana,providing a foundation for future research in agricultural applications.The microbial species identified,particularly siderophoreproducing fungi,could have significant implications for improving soil fertility and promoting sustainable agricultural practices.The use of NGS technology has enabled comprehensive profiling of microbial communities,allowing for precise identification of fungi that may play essential roles in soil health.Furthermore,the study paves the way for future studies on the potential applications of earthworm gut microbiomes in biotechnology,especially in enhancing soil nutrient availability and plant growth.The findings of this research contribute to the broader understanding of the ecological roles of earthworms and their microbiomes in soil ecosystems.
